Rroxscaffold_5G00333020

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011327
Physical Location & Seq
Forward (+)
473511 .. 481694
8184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333020.1

Sequence Viewer

Length: 2361 bp
ATGGAGGCAGCGCCTCAGCTCGTCTCCACTGGAATCGAATCCCTTCGCCGGAGCTCCAAGCATTCATTCCCGACCACAACCTCCAGTAGAGTTCGAGTTCCGAAACGATTTAACCGGGTTCTTGCGGTTGCCACCAAGTCCGGCTCATCACCATCAAAACCCAAATCTCCATCGCCAAACTCTGTCAATGGCTCCTCCTCGAGGTCCCCGCCTATCAATGGAGTTTCCACGAGAATTGGAGATGTTTCAAAGGAAATCAAAAGGGTGCGAGCACAGATGGAAGAAAACGAAGACTTGGCAATACTCATGAAAGGTCTTCGCGGACAAAATTTGAAGGATTCACAGTTTGCTGAGGATGATGTTCAGCTTCGTCTTGTTGAGGTAGATGAAAGCAGTGAATTTTTGCCTGTGGTATATGATCCTGCTAGCATCTCTGCTTATTGGGGAAAACGGCCTCGTGCTGTTGTGACACGTGTAATTCAATTACTCTCTGTAACTGGGGGTTTCCTTTCACGTCTTGCAGGGGATTTTATAAACAAGAAACTCAAAGAGAATGAAGTTGCTAGGGCCATTGAAATAAGGGAAATTGTGACCTCTTTGGGTCCCGCATATATCAAACTTGGGCAAGCATTGAGCATTCGACCAGATATACTGTCACCCGCTGCAATGACTGAGCTGCAAAAGCTTTGTGATAAGGTTCCTTCATTTCCGGATGATATAGCTATGGCTCTCATTGAGGAGGAGCTTGGTCAGCCATGGCAAAACATCTACTCTGAGCTCTCTTCCTCTCCAATTGCTGCTGCATCTCTTGGGCAAGTATATAAGGGCCGGCTAAAAGAAAATGGAGATCTCGTGGCTGTTAAAGTGCAGAGACCTTTTGTTCTTGAAACAGTAACTGTTGATTTGTTTATCATACGGAACTTGGGTTTGGTTCTCCGGAGGTTTCCTCAGATCTCTGTAGATGTGGTTGGATTAGTTGATGAATGGGCAGCTCGTTTCTTTGAAGAGTTGGATTATATTAATGAGGGTGAGAATGGACTACTCTTTGCTGAAATGATGAGAAAAGATCTTCCACAGGTGGTTGTACCAAAAACCTACCCAAAATATACATCAAGAAAGGTTCTTACTACATCATGGTTAGAAGGAGAGAAGCTATCACAAAGTACTGAAAGTGATGTTGGAGAACTGGTCAATGTTGGAGTCATATGCTACCTGAAGCAGTTGCTTGACACTGGGTTATTTCATGCTGATCCTCATCCTGGGAATTTGATTCGCACTCCAGATGGCAAGCTTGCCATACTTGATTTTGGTCTGGTCACCAAGTTAACTGATGATCAAAAGTATGGAATGATCGAGGCAATTGCTCACCTTATTCATCGTGATTATGGAGCCATAGTCAAAGACTTTGTCAAGCTTGGTTTTATTGACGAGGGGGTGAATCTAGAACCTATTTTGCCTGTTCTGGCCAAGGTTTTTGATCAGGCTCTTGAAGGTGGAGGAGCAAAAAATATCAACTTCCAAGACTTGGCAGCAGATTTGGCACAAATCACATTTGACTATCCATTTAAGATACCGCCTTATTTCGCTCTTATAATTAGAGCAATTGGGGTCCTGGAAGGCATAGCTTTAGTGGGAAATCCTGATTTTGCTATTGTGGATGAGGCCTATCCATATATTGCCCAGAGGCTACTGACAGATGAATCCCCACGGTTAAGGAATGCTTTGCGTTACACAATTTATGGGAAATCTGGGGTTTTTGATGCGGAAAGATTTATTGATGTCATGCAAGCCTTTGAGAGTTTCATAACTGCTGCTAAAAGTGGAGGTGGAGAGGAAATGAATGGCGATATGGCTGAACTTGGACTTTTGCAAAGTCGAACAGAGTATACGCTTCCTGGATTTGCATCAAATGTGCAACCAGTTCAAACAAGGGCGGCCTTAGCATTTGTACTGTCTGATAAAGGAAACTTTTTCAGAGAATTTCTTCTGGATGAGATTGTGAAAGGCATTGATGCAGTTACAAGAGAACAATTGGTTCGTGCAATGGCACTTCTTGGATTCGGGAATGCTCTTCCAGTTTTCAGCATGGCTCCTAGTTTTGGGCCTTTCAAGCCTGCAGGACTTCTACCAACAATAACTGAGGAGGATAGAGTTATATTGAACAATGTTCAAAGAGTAGTCGAGTTCCTGGCTGCAGGGAGTTCGATATCAAGAACATCCAATCAGGAATTAAATGTGTCTCAGGTTATCCAAGATTTTCTTCCTGTTATGCCGAGTATCTCTTCCAATGTGCTCCCTGAAGTGCTCAGCCGATTATCTTCACGGGTAATAGCACGTGTGATTAGAGATACAATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH

Protein Analysis

786

Amino Acids

86.48

Weight (kDa)

5.4

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 229 - 467 5.2e-63 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 533, 1592
AbsI CCTCGAGG 1 cut(s) 199
AccB7I CCANNNNNTGG 1 cut(s) 1525
AccI GTMKAC 1 cut(s) 1886
AccII CGCG 1 cut(s) 321
AccIII TCCGGA 2 cut(s) 709, 936
AciI CCGC 8 cut(s) 125, 209, 321, 606, 660, 1574, 1763, 1934
AclWI GGATC 2 cut(s) 413, 1244
AcoI YGGCCR 1 cut(s) 1464
AcsI RAATTY 4 cut(s) 328, 398, 1264, 1979
AcuI CTGAAG 2 cut(s) 1235, 2319
AcvI CACGTG 2 cut(s) 473, 2336
AfaI GTAC 3 cut(s) 1086, 1165, 1950
AfiI CCNNNNNNNGG 6 cut(s) 48, 201, 218, 1259, 1525, 2099
AflIII ACRYGT 3 cut(s) 470, 472, 2335
AjiI CACGTC 1 cut(s) 515
AjnI CCWGG 4 cut(s) 1258, 1611, 1894, 2187
AjuI GAANNNNNNNTTGG 8 cut(s) 50, 82, 911, 943, 1161, 1193, 2244, 2276
Alw21I GWGCWC 5 cut(s) 56, 274, 780, 2295, 2307
Alw26I GTCTC 3 cut(s) 28, 865, 2244
AlwI GGATC 2 cut(s) 413, 1244
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 1 cut(s) 199
Aor13HI TCCGGA 2 cut(s) 709, 936
AoxI GGCC 7 cut(s) 452, 567, 826, 1464, 1662, 1935, 2102
ApeKI GCWGC 9 cut(s) 8, 662, 676, 797, 800, 989, 1529, 1811, 2192
ApoI RAATTY 4 cut(s) 328, 398, 1264, 1979
ArsI GACNNNNNNTTYG 2 cut(s) 1392, 1424
AseI ATTAAT 1 cut(s) 1020
Asp700I GAANNNNTTC 2 cut(s) 42, 1983
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 6 cut(s) 204, 567, 602, 826, 1609, 2102
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 6 cut(s) 141, 648, 1040, 1309, 1358, 1447
AsuNHI GCTAGC 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 199
AvaII GGWCC 3 cut(s) 204, 602, 1609
BalI TGGCCA 1 cut(s) 1466
BanII GRGCYC 2 cut(s) 56, 780
BauI CACGAG 3 cut(s) 229, 456, 851
BbrPI CACGTG 2 cut(s) 473, 2336
BbsI GAAGAC 2 cut(s) 297, 308
Bbv12I GWGCWC 5 cut(s) 56, 274, 780, 2295, 2307
BbvCI CCTCAGC 2 cut(s) 15, 351
BbvI GCAGC 9 cut(s) 20, 649, 663, 784, 787, 1001, 1541, 1798, 2179
BccI CCATC 4 cut(s) 160, 178, 271, 1277
BceAI ACGGC 1 cut(s) 467
BcgI CGANNNNNNTGC 4 cut(s) 1343, 1377, 2184, 2218
BciT130I CCWGG 4 cut(s) 1260, 1613, 1896, 2189
BclI TGATCA 2 cut(s) 1333, 1477
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 3 cut(s) 28, 865, 2244
BfaI CTAG 4 cut(s) 426, 564, 1442, 2094
BfmI CTRYAG 3 cut(s) 957, 2115, 2193
BfoI RGCGCY 1 cut(s) 14
BglII AGATCT 3 cut(s) 847, 951, 1066
BlpI GCTNAGC 1 cut(s) 2306
BmcAI AGTACT 1 cut(s) 1165
Bme1390I CCNGG 5 cut(s) 116, 1260, 1613, 1896, 2189
Bme18I GGWCC 3 cut(s) 204, 602, 1609
BmeT110I CYCGRG 1 cut(s) 199
BmgBI CACGTC 1 cut(s) 515
BmgT120I GGNCC 6 cut(s) 204, 567, 602, 826, 1609, 2102
BmiI GGNNCC 8 cut(s) 193, 206, 603, 604, 699, 1390, 1610, 2091
BmrFI CCNGG 5 cut(s) 116, 1260, 1613, 1896, 2189
BmrI ACTGGG 2 cut(s) 507, 1242
BmsI GCATC 5 cut(s) 438, 812, 1750, 1913, 2002
BmtI GCTAGC 1 cut(s) 429
BmuI ACTGGG 2 cut(s) 507, 1242
BpiI GAAGAC 2 cut(s) 297, 308
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 2 cut(s) 67, 1263
Bpu10I CCTNAGC 3 cut(s) 15, 351, 1939
Bpu1102I GCTNAGC 1 cut(s) 2306
BpuMI CCSGG 1 cut(s) 116
BsaAI YACGTR 2 cut(s) 473, 2336
BsaBI GATNNNNATC 2 cut(s) 1254, 1903
BsaI GGTCTC 1 cut(s) 865
BsaJI CCNNGG 4 cut(s) 755, 1259, 1467, 1706
BsaWI WCCGGW 2 cut(s) 709, 936
BsaXI ACNNNNNCTCC 6 cut(s) 837, 867, 1173, 1203, 1380, 1410
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 201, 218, 1259, 1525, 2099
Bse118I RCCGGY 1 cut(s) 828
Bse1I ACTGG 7 cut(s) 34, 84, 502, 1191, 1237, 1919, 2075
Bse3DI GCAATG 2 cut(s) 672, 2049
Bse8I GATNNNNATC 2 cut(s) 1254, 1903
BseAI TCCGGA 2 cut(s) 709, 936
BseBI CCWGG 4 cut(s) 1260, 1613, 1896, 2189
BseDI CCNNGG 4 cut(s) 755, 1259, 1467, 1706
BseGI GGATG 6 cut(s) 361, 718, 1255, 1663, 1996, 2216
BseJI GATNNNNATC 2 cut(s) 1254, 1903
BseLI CCNNNNNNNGG 6 cut(s) 48, 201, 218, 1259, 1525, 2099
BseMI GCAATG 2 cut(s) 672, 2049
BseMII CTCAG 8 cut(s) 29, 342, 663, 765, 962, 2130, 2255, 2320
BseNI ACTGG 7 cut(s) 34, 84, 502, 1191, 1237, 1919, 2075
BseRI GAGGAG 6 cut(s) 184, 187, 752, 755, 1512, 2156
BseXI GCAGC 9 cut(s) 20, 649, 663, 784, 787, 1001, 1541, 1798, 2179
BsgI GTGCAG 1 cut(s) 887
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 7 cut(s) 454, 569, 828, 1466, 1664, 1937, 2104
BsiHKAI GWGCWC 5 cut(s) 56, 274, 780, 2295, 2307
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 6 cut(s) 49, 115, 141, 710, 829, 937
BslFI GGGAC 2 cut(s) 190, 588
BslI CCNNNNNNNGG 6 cut(s) 48, 201, 218, 1259, 1525, 2099
BsmAI GTCTC 3 cut(s) 28, 865, 2244
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 2 cut(s) 190, 588
BsmI GAATGC 4 cut(s) 61, 636, 1723, 2071
BsnI GGCC 7 cut(s) 454, 569, 828, 1466, 1664, 1937, 2104
Bso31I GGTCTC 1 cut(s) 865
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 5 cut(s) 56, 274, 780, 2295, 2307
Bsp13I TCCGGA 2 cut(s) 709, 936
Bsp143I GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
Bsp1720I GCTNAGC 1 cut(s) 2306
Bsp19I CCATGG 1 cut(s) 755
BspACI CCGC 8 cut(s) 125, 209, 321, 606, 660, 1574, 1763, 1934
BspANI GGCC 7 cut(s) 454, 569, 828, 1466, 1664, 1937, 2104
BspCNI CTCAG 8 cut(s) 28, 343, 664, 766, 961, 2131, 2254, 2319
BspEI TCCGGA 2 cut(s) 709, 936
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 8 cut(s) 193, 206, 603, 604, 699, 1390, 1610, 2091
BspMAI CTGCAG 2 cut(s) 2119, 2197
BspOI GCTAGC 1 cut(s) 429
BspPI GGATC 2 cut(s) 413, 1244
BspQI GCTCTTC 1 cut(s) 2076
BspTNI GGTCTC 1 cut(s) 865
BsrDI GCAATG 2 cut(s) 672, 2049
BsrFI RCCGGY 1 cut(s) 828
BsrI ACTGG 7 cut(s) 34, 84, 502, 1191, 1237, 1919, 2075
BssAI RCCGGY 1 cut(s) 828
BssECI CCNNGG 4 cut(s) 755, 1259, 1467, 1706
BssMI GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
BssNAI GTATAC 1 cut(s) 1887
BssSI CACGAG 3 cut(s) 229, 456, 851
BssT1I CCWWGG 2 cut(s) 755, 1467
Bst1107I GTATAC 1 cut(s) 1887
Bst2BI CACGAG 3 cut(s) 229, 456, 851
Bst2UI CCWGG 4 cut(s) 1260, 1613, 1896, 2189
Bst4CI ACNGT 6 cut(s) 345, 654, 892, 898, 1710, 1953
Bst6I CTCTTC 4 cut(s) 787, 999, 2076, 2287
BstBAI YACGTR 2 cut(s) 473, 2336
BstC8I GCNNGC 8 cut(s) 270, 427, 627, 830, 1289, 1293, 1788, 2115
BstDEI CTNAG 9 cut(s) 15, 351, 672, 774, 948, 1939, 2139, 2241, 2306
BstDSI CCRYGG 2 cut(s) 755, 1706
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 1 cut(s) 199
BstF5I GGATG 6 cut(s) 361, 718, 1255, 1663, 1996, 2216
BstFNI CGCG 1 cut(s) 321
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 8 cut(s) 421, 850, 954, 1069, 1252, 1336, 1353, 1480
BstMAI GTCTC 3 cut(s) 28, 865, 2244
BstMBI GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
BstMWI GCNNNNNNNGC 5 cut(s) 682, 751, 1538, 1940, 2110
BstNI CCWGG 4 cut(s) 1260, 1613, 1896, 2189
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 5 cut(s) 114, 1258, 1611, 1894, 2187
BstSFI CTRYAG 3 cut(s) 957, 2115, 2193
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 9 cut(s) 20, 649, 663, 784, 787, 1001, 1541, 1798, 2179
BstV2I GAAGAC 2 cut(s) 297, 308
BstX2I RGATCY 3 cut(s) 847, 951, 1066
BstYI RGATCY 3 cut(s) 847, 951, 1066
BstZ17I GTATAC 1 cut(s) 1887
BsuRI GGCC 7 cut(s) 454, 569, 828, 1466, 1664, 1937, 2104
BtgI CCRYGG 2 cut(s) 755, 1706
BtgZI GCGATG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 515
BtsCI GGATG 6 cut(s) 361, 718, 1255, 1663, 1996, 2216
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 3 cut(s) 27, 400, 1230
Cac8I GCNNGC 8 cut(s) 270, 427, 627, 830, 1289, 1293, 1788, 2115
CaiI CAGNNNCTG 1 cut(s) 896
CciI TCATGA 1 cut(s) 306
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 828
Cfr13I GGNCC 6 cut(s) 204, 567, 602, 826, 1609, 2102
Csp6I GTAC 3 cut(s) 1085, 1164, 1949
CspCI CAANNNNNGTGG 2 cut(s) 217, 252
CviAII CATG 6 cut(s) 307, 756, 1134, 1244, 1783, 2086
CviQI GTAC 3 cut(s) 1085, 1164, 1949
DdeI CTNAG 9 cut(s) 15, 351, 672, 774, 948, 1939, 2139, 2241, 2306
DpnI GATC 8 cut(s) 420, 849, 953, 1068, 1251, 1335, 1352, 1479
DpnII GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
EaeI YGGCCR 1 cut(s) 1464
Eam1104I CTCTTC 4 cut(s) 787, 999, 2076, 2287
EarI CTCTTC 4 cut(s) 787, 999, 2076, 2287
Ecl136II GAGCTC 2 cut(s) 54, 778
Eco130I CCWWGG 2 cut(s) 755, 1467
Eco147I AGGCCT 1 cut(s) 1664
Eco24I GRGCYC 2 cut(s) 56, 780
Eco31I GGTCTC 1 cut(s) 865
Eco32I GATATC 1 cut(s) 2208
Eco47I GGWCC 3 cut(s) 204, 602, 1609
Eco53kI GAGCTC 2 cut(s) 54, 778
Eco57I CTGAAG 2 cut(s) 1235, 2319
Eco72I CACGTG 2 cut(s) 473, 2336
Eco88I CYCGRG 1 cut(s) 199
Eco91I GGTNACC 1 cut(s) 1315
EcoICRI GAGCTC 2 cut(s) 54, 778
EcoNI CCTNNNNNAGG 1 cut(s) 199
EcoO109I RGGNCCY 3 cut(s) 204, 602, 1609
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 4 cut(s) 1258, 1611, 1894, 2187
EcoRV GATATC 1 cut(s) 2208
EcoT14I CCWWGG 2 cut(s) 755, 1467
EcoT38I GRGCYC 2 cut(s) 56, 780
ErhI CCWWGG 2 cut(s) 755, 1467
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 6 cut(s) 310, 759, 1137, 1247, 1786, 2089
FalI AAGNNNNNCTT 4 cut(s) 1705, 1737, 2244, 2276
FaqI GGGAC 2 cut(s) 190, 588
FatI CATG 6 cut(s) 306, 755, 1133, 1243, 1782, 2085
FauI CCCGC 3 cut(s) 216, 613, 667
FauNDI CATATG 1 cut(s) 1205
FbaI TGATCA 2 cut(s) 1333, 1477
FblI GTMKAC 1 cut(s) 1886
FokI GGATG 6 cut(s) 368, 725, 1242, 1670, 2003, 2203
FriOI GRGCYC 2 cut(s) 56, 780
FspBI CTAG 4 cut(s) 426, 564, 1442, 2094
GlaI GCGC 1 cut(s) 12
GsuI CTGGAG 2 cut(s) 67, 1263
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 7 cut(s) 454, 569, 828, 1466, 1664, 1937, 2104
HapII CCGG 6 cut(s) 49, 115, 141, 710, 829, 937
HhaI GCGC 1 cut(s) 13
Hin1II CATG 6 cut(s) 310, 759, 1137, 1247, 1786, 2089
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 1326
HindII GTYRAC 1 cut(s) 1326
HindIII AAGCTT 3 cut(s) 683, 1289, 1412
HinfI GANTC 8 cut(s) 33, 38, 338, 1200, 1270, 1438, 1700, 2058
HpaI GTTAAC 1 cut(s) 1326
HpaII CCGG 6 cut(s) 49, 115, 141, 710, 829, 937
HphI GGTGA 6 cut(s) 141, 648, 1040, 1309, 1358, 1447
Hpy166II GTNNAC 2 cut(s) 1326, 1887
Hpy188I TCNGA 5 cut(s) 102, 775, 951, 1957, 1976
Hpy8I GTNNAC 2 cut(s) 1326, 1887
HpyAV CCTTC 6 cut(s) 53, 328, 711, 1136, 1484, 1610
HpyCH4III ACNGT 6 cut(s) 345, 654, 892, 898, 1710, 1953
HpyCH4IV ACGT 3 cut(s) 472, 514, 2335
HpyF10VI GCNNNNNNNGC 5 cut(s) 682, 751, 1538, 1940, 2110
HpyF3I CTNAG 9 cut(s) 15, 351, 672, 774, 948, 1939, 2139, 2241, 2306
HpySE526I ACGT 3 cut(s) 472, 514, 2335
Hsp92II CATG 6 cut(s) 310, 759, 1137, 1247, 1786, 2089
HspAI GCGC 1 cut(s) 11
KflI GGGWCCC 1 cut(s) 602
Kpn2I TCCGGA 2 cut(s) 709, 936
KroI GCCGGC 1 cut(s) 828
KroNI GCCGGC 1 cut(s) 830
Ksp22I TGATCA 2 cut(s) 1333, 1477
KspAI GTTAAC 1 cut(s) 1326
Kzo9I GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
LguI GCTCTTC 1 cut(s) 2076
LmnI GCTCC 8 cut(s) 51, 59, 197, 742, 1388, 1499, 2095, 2298
Lsp1109I GCAGC 9 cut(s) 20, 649, 663, 784, 787, 1001, 1541, 1798, 2179
LweI GCATC 5 cut(s) 438, 812, 1750, 1913, 2002
MaeI CTAG 4 cut(s) 426, 564, 1442, 2094
MaeII ACGT 3 cut(s) 472, 514, 2335
MaeIII GTNAC 8 cut(s) 466, 493, 589, 654, 892, 1315, 1727, 2017
MalI GATC 8 cut(s) 420, 849, 953, 1068, 1251, 1335, 1352, 1479
MboI GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
MfeI CAATTG 4 cut(s) 792, 1359, 1602, 2030
MflI RGATCY 3 cut(s) 847, 951, 1066
MhlI GDGCHC 5 cut(s) 56, 274, 780, 2295, 2307
MlsI TGGCCA 1 cut(s) 1466
MluNI TGGCCA 1 cut(s) 1466
MlyI GAGTC 1 cut(s) 1209
MmeI TCCRAC 4 cut(s) 949, 990, 1159, 1177
Mox20I TGGCCA 1 cut(s) 1466
MroI TCCGGA 2 cut(s) 709, 936
MroNI GCCGGC 1 cut(s) 828
MroXI GAANNNNTTC 2 cut(s) 42, 1983
MscI TGGCCA 1 cut(s) 1466
MseI TTAA 7 cut(s) 111, 861, 1020, 1325, 1566, 1712, 2231
Msp20I TGGCCA 1 cut(s) 1466
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 6 cut(s) 49, 115, 141, 710, 829, 937
MspR9I CCNGG 5 cut(s) 116, 1260, 1613, 1896, 2189
MunI CAATTG 4 cut(s) 792, 1359, 1602, 2030
Mva1269I GAATGC 4 cut(s) 61, 636, 1723, 2071
MvaI CCWGG 4 cut(s) 1260, 1613, 1896, 2189
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 5 cut(s) 682, 751, 1538, 1940, 2110
NaeI GCCGGC 1 cut(s) 830
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 755
NdeI CATATG 1 cut(s) 1205
NdeII GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
NgoMIV GCCGGC 1 cut(s) 828
NheI GCTAGC 1 cut(s) 425
NlaIII CATG 6 cut(s) 310, 759, 1137, 1247, 1786, 2089
NlaIV GGNNCC 8 cut(s) 193, 206, 603, 604, 699, 1390, 1610, 2091
NmeAIII GCCGAG 1 cut(s) 2298
NmuCI GTSAC 4 cut(s) 466, 589, 654, 1315
PaeR7I CTCGAG 1 cut(s) 199
PagI TCATGA 1 cut(s) 306
PceI AGGCCT 1 cut(s) 1664
PciSI GCTCTTC 1 cut(s) 2076
PctI GAATGC 4 cut(s) 61, 636, 1723, 2071
PdiI GCCGGC 1 cut(s) 830
PdmI GAANNNNTTC 2 cut(s) 42, 1983
PfeI GAWTC 7 cut(s) 33, 38, 338, 1270, 1438, 1700, 2058
PflFI GACNNNGTC 1 cut(s) 1406
PflMI CCANNNNNTGG 1 cut(s) 1525
PfoI TCCNGGA 2 cut(s) 1611, 1894
PleI GAGTC 1 cut(s) 1208
PmaCI CACGTG 2 cut(s) 473, 2336
PmlI CACGTG 2 cut(s) 473, 2336
PpsI GAGTC 1 cut(s) 1208
Ppu21I YACGTR 2 cut(s) 473, 2336
PpuMI RGGWCCY 3 cut(s) 204, 602, 1609
PshBI ATTAAT 1 cut(s) 1020
PsiI TTATAA 2 cut(s) 533, 1592
Psp124BI GAGCTC 2 cut(s) 56, 780
Psp5II RGGWCCY 3 cut(s) 204, 602, 1609
Psp6I CCWGG 4 cut(s) 1258, 1611, 1894, 2187
PspCI CACGTG 2 cut(s) 473, 2336
PspEI GGTNACC 1 cut(s) 1315
PspGI CCWGG 4 cut(s) 1258, 1611, 1894, 2187
PspN4I GGNNCC 8 cut(s) 193, 206, 603, 604, 699, 1390, 1610, 2091
PspPI GGNCC 6 cut(s) 204, 567, 602, 826, 1609, 2102
PspPPI RGGWCCY 3 cut(s) 204, 602, 1609
PspXI VCTCGAGB 1 cut(s) 199
PsrI GAACNNNNNNTAC 2 cut(s) 1870, 1902
PstI CTGCAG 2 cut(s) 2119, 2197
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 3 cut(s) 847, 951, 1066
PsyI GACNNNGTC 1 cut(s) 1406
RsaI GTAC 3 cut(s) 1086, 1165, 1950
RsaNI GTAC 3 cut(s) 1085, 1164, 1949
SacI GAGCTC 2 cut(s) 56, 780
SapI GCTCTTC 1 cut(s) 2076
SaqAI TTAA 7 cut(s) 111, 861, 1020, 1325, 1566, 1712, 2231
Sau3AI GATC 8 cut(s) 418, 847, 951, 1066, 1249, 1333, 1350, 1477
Sau96I GGNCC 6 cut(s) 204, 567, 602, 826, 1609, 2102
SbfI CCTGCAGG 1 cut(s) 2119
ScaI AGTACT 1 cut(s) 1165
SchI GAGTC 1 cut(s) 1209
ScrFI CCNGG 5 cut(s) 116, 1260, 1613, 1896, 2189
SdaI CCTGCAGG 1 cut(s) 2119
SduI GDGCHC 5 cut(s) 56, 274, 780, 2295, 2307
SfaNI GCATC 5 cut(s) 438, 812, 1750, 1913, 2002
SfcI CTRYAG 3 cut(s) 957, 2115, 2193
Sfr274I CTCGAG 1 cut(s) 199
SinI GGWCC 3 cut(s) 204, 602, 1609
SlaI CTCGAG 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse8387I CCTGCAGG 1 cut(s) 2119
SseBI AGGCCT 1 cut(s) 1664
SsiI CCGC 8 cut(s) 125, 209, 321, 606, 660, 1574, 1763, 1934
SspMI CTAG 4 cut(s) 426, 564, 1442, 2094
SstI GAGCTC 2 cut(s) 56, 780
StuI AGGCCT 1 cut(s) 1664
StyD4I CCNGG 5 cut(s) 114, 1258, 1611, 1894, 2187
StyI CCWWGG 2 cut(s) 755, 1467
TaaI ACNGT 6 cut(s) 345, 654, 892, 898, 1710, 1953
TaiI ACGT 3 cut(s) 475, 517, 2338
TaqI TCGA 8 cut(s) 36, 94, 200, 640, 1353, 1876, 2181, 2204
TatI WGTACW 2 cut(s) 1163, 1948
TauI GCSGC 1 cut(s) 1937
TfiI GAWTC 7 cut(s) 33, 38, 338, 1270, 1438, 1700, 2058
Tru1I TTAA 7 cut(s) 111, 861, 1020, 1325, 1566, 1712, 2231
Tru9I TTAA 7 cut(s) 111, 861, 1020, 1325, 1566, 1712, 2231
TscAI CASTG 3 cut(s) 34, 400, 1237
TseFI GTSAC 4 cut(s) 466, 589, 654, 1315
TseI GCWGC 9 cut(s) 8, 662, 676, 797, 800, 989, 1529, 1811, 2192
Tsp45I GTSAC 4 cut(s) 466, 589, 654, 1315
TspGWI ACGGA 1 cut(s) 931
TspRI CASTG 3 cut(s) 34, 400, 1237
Tth111I GACNNNGTC 1 cut(s) 1406
Van91I CCANNNNNTGG 1 cut(s) 1525
VpaK11BI GGWCC 3 cut(s) 204, 602, 1609
VspI ATTAAT 1 cut(s) 1020
XagI CCTNNNNNAGG 1 cut(s) 199
XapI RAATTY 4 cut(s) 328, 398, 1264, 1979
XbaI TCTAGA 1 cut(s) 1441
XhoI CTCGAG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 1886
XmnI GAANNNNTTC 2 cut(s) 42, 1983
XspI CTAG 4 cut(s) 426, 564, 1442, 2094
ZrmI AGTACT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.