MD04G1007300.v1.1

Protein farnesyltransferase geranylgeranyltransferase type-1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012140
Physical Location & Seq
Forward (+)
850524 .. 853424
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1007300.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAGAGAGAGAGAAATGGACTCGGACGAAGAACAACTGTGGGTCCCATTCAAGAGTAGGCCGGAATGGTCGGACGTGATTCCGGTCGAGCAAGACGACGGTCCGAACCCGGTCGTCCCCATAGCCTACAAGGAACAGTTCACAGAGACCATGAACTACTTCCGAGCCGTCTTCAAGGCCGACGAGCGATCGCCTCGCGCCCTTCAGCTCACCACCGAAGCCATCGGATTGAATTCAGGAAACTACACTATATGGCATTACAGGCGCTTAATACTGGAGACCCTTAAAGCTGATTTACAGAATGAACTTGATTTCACCGATCAGATTGCGCAGATTAACTCCAAAAATTATCAGTTGTGGCATCATAGGCGGTGGGTTGCTGAGATATTGGGAACTAATGCTACAAGTGCGGAACTTGAGTTTACAAAGAAGATACTCTTGGGTGATGCTAAAAACTATCATGCTTGGTCTCATAGGCAGTGGGTTCTCAAGGAACTTGGAGGGTGGGAAAATGAACTTGACTATTGTCAGGAGCTCCTTGAAGAAGACGTTTTAAACAATTCTGCTTGGAATCAGAGATATTTCGTCATTACAAGATCTCCACTCCTGGGAGGCCTAACAGCTATGAGAGAGTCTGAAGTGTCTTATACTCTTAAAGCCATTGTAGCACACCCAGAGAATGAGAGTTCATGGAGATACCTACGAGGGCTTTACAAAAACGACAACTGGTCTTGGGCAAACGACCCTCAAGTTGCTTCGGTATGCTTGAAGGTTTTAAGTGCCAACAACGACTGTATCTTTGCTTTGAGCACACTTTTGGATCTCATCCGCCATGGTTTCCAAGCAAGCGAAGAGCTCAGAGATGCTGTGGATGCTATAAGAAACTCAAACTTGGTAGAAGCAGAATCGGACTTGACAAAAACTATTTGCTCGCTCTTGGAAAAGCTTGATCCGATAAGAGTTAACTATTGGAAGTGGCGTAAGAGCATCCTCTGCGAAGGTGCTAAATAG

Protein Analysis

337

Amino Acids

39.12

Weight (kDa)

5.13

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPTA PF01239 66 - 94 1.8e-07 Protein prenyltransferase alpha subunit repeat
PPTA PF01239 99 - 128 9.2e-11 Protein prenyltransferase alpha subunit repeat
PPTA PF01239 137 - 165 6.4e-10 Protein prenyltransferase alpha subunit repeat
PPTA PF01239 169 - 199 4.8e-07 Protein prenyltransferase alpha subunit repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59380
fragaria_vesca FvH4_4g10680 FvH4_4g10680 FvH4_4g10680 FvH4_4g10680 FvH4_4g10680
malus_domestica MD04G1007300.v1.1
prunus_persica Prupe.1G191500_v2.0.a1
pyrus_communis pycom04g03430
rosa_chinensis RchiOBHm_Chr4g0407031
rosa_laevigata RLG00000008742
rosa_multiflora Rmu_sc0000600.1_g000009
rosa_roxburghii Rroxscaffold_5G00351730
rosa_rugosa Rorug04G0077000
rosa_samantha Rh4AG142200 Rh4BG139400 Rh4CG148800 Rh4DG136400
rosa_wichuraiana Rw4G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 330
AccII CGCG 1 cut(s) 198
AciI CCGC 3 cut(s) 370, 410, 829
AclWI GGATC 2 cut(s) 828, 944
AcsI RAATTY 1 cut(s) 232
AcuI CTGAAG 2 cut(s) 188, 657
AfiI CCNNNNNNNGG 1 cut(s) 608
AgsI TTSAA 5 cut(s) 52, 175, 232, 542, 769
AhdI GACNNNNNGTC 1 cut(s) 727
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 606
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 6 cut(s) 208, 290, 535, 623, 856, 946
AluI AGCT 6 cut(s) 208, 290, 535, 623, 856, 946
Alw21I GWGCWC 3 cut(s) 537, 812, 858
Alw26I GTCTC 3 cut(s) 140, 272, 474
AlwI GGATC 2 cut(s) 828, 944
AoxI GGCC 3 cut(s) 59, 177, 613
ApoI RAATTY 1 cut(s) 232
ArsI GACNNNNNNTTYG 2 cut(s) 782, 814
AsiSI GCGATCGC 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 3 cut(s) 200, 267, 331
AspS9I GGNCC 2 cut(s) 43, 101
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 3 cut(s) 202, 307, 455
AvaII GGWCC 2 cut(s) 43, 101
BaeI ACNNNNGTAYC 2 cut(s) 778, 811
BanII GRGCYC 2 cut(s) 537, 858
BbsI GAAGAC 2 cut(s) 163, 552
Bbv12I GWGCWC 3 cut(s) 537, 812, 858
BccI CCATC 1 cut(s) 230
BceAI ACGGC 1 cut(s) 152
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 608
BcnI CCSGG 1 cut(s) 110
BcoDI GTCTC 3 cut(s) 140, 272, 474
BfoI RGCGCY 1 cut(s) 268
BglII AGATCT 1 cut(s) 596
Bme1390I CCNGG 2 cut(s) 110, 608
Bme18I GGWCC 2 cut(s) 43, 101
BmeRI GACNNNNNGTC 1 cut(s) 727
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 43, 101
BmiI GGNNCC 2 cut(s) 44, 45
BmrFI CCNGG 2 cut(s) 110, 608
BmsI GCATC 5 cut(s) 370, 436, 853, 862, 996
BoxI GACNNNNGTC 2 cut(s) 99, 525
BpiI GAAGAC 2 cut(s) 163, 552
BpmI CTGGAG 1 cut(s) 296
BpuEI CTTGAG 3 cut(s) 437, 473, 732
BpuMI CCSGG 1 cut(s) 110
BsaI GGTCTC 3 cut(s) 140, 272, 474
BsaJI CCNNGG 2 cut(s) 607, 832
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 583, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse1I ACTGG 2 cut(s) 279, 731
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 2 cut(s) 607, 832
BseGI GGATG 3 cut(s) 825, 877, 987
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMII CTCAG 2 cut(s) 372, 871
BseNI ACTGG 2 cut(s) 279, 731
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 3 cut(s) 87, 114, 191
BshFI GGCC 3 cut(s) 61, 179, 615
BsiEI CGRYCG 3 cut(s) 87, 114, 191
BsiHKAI GWGCWC 3 cut(s) 537, 812, 858
BsiSI CCGG 3 cut(s) 62, 83, 110
BslFI GGGAC 2 cut(s) 29, 101
BslI CCNNNNNNNGG 1 cut(s) 608
BsmAI GTCTC 3 cut(s) 140, 272, 474
BsmFI GGGAC 2 cut(s) 29, 101
BsnI GGCC 3 cut(s) 61, 179, 615
Bso31I GGTCTC 3 cut(s) 140, 272, 474
Bsp1286I GDGCHC 3 cut(s) 537, 812, 858
Bsp143I GATC 5 cut(s) 188, 319, 596, 820, 949
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 3 cut(s) 370, 410, 829
BspANI GGCC 3 cut(s) 61, 179, 615
BspCNI CTCAG 2 cut(s) 373, 870
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 2 cut(s) 44, 45
BspPI GGATC 2 cut(s) 828, 944
BspQI GCTCTTC 1 cut(s) 846
BspTNI GGTCTC 3 cut(s) 140, 272, 474
BsrI ACTGG 2 cut(s) 279, 731
BssECI CCNNGG 2 cut(s) 607, 832
BssMI GATC 5 cut(s) 188, 319, 596, 820, 949
BssT1I CCWWGG 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 4 cut(s) 39, 101, 138, 794
Bst6I CTCTTC 1 cut(s) 846
BstAPI GCANNNNNTGC 1 cut(s) 993
BstC8I GCNNGC 2 cut(s) 847, 932
BstDEI CTNAG 2 cut(s) 381, 857
BstDSI CCRYGG 1 cut(s) 832
BstF5I GGATG 3 cut(s) 825, 877, 987
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 3 cut(s) 200, 267, 331
BstKTI GATC 5 cut(s) 191, 322, 599, 823, 952
BstMAI GTCTC 3 cut(s) 140, 272, 474
BstMBI GATC 5 cut(s) 188, 319, 596, 820, 949
BstMCI CGRYCG 3 cut(s) 87, 114, 191
BstMWI GCNNNNNNNGC 6 cut(s) 262, 367, 407, 665, 872, 993
BstNI CCWGG 1 cut(s) 608
BstPAI GACNNNNGTC 2 cut(s) 99, 525
BstSCI CCNGG 2 cut(s) 108, 606
BstUI CGCG 1 cut(s) 198
BstV2I GAAGAC 2 cut(s) 163, 552
BstX2I RGATCY 2 cut(s) 596, 820
BstYI RGATCY 2 cut(s) 596, 820
BsuRI GGCC 3 cut(s) 61, 179, 615
BtgI CCRYGG 1 cut(s) 832
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 3 cut(s) 825, 877, 987
BtsI GCAGTG 1 cut(s) 485
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 847, 932
CfoI GCGC 3 cut(s) 200, 267, 331
Cfr13I GGNCC 2 cut(s) 43, 101
CpoI CGGWCCG 1 cut(s) 101
CspI CGGWCCG 1 cut(s) 101
CviAII CATG 4 cut(s) 151, 461, 690, 833
DdeI CTNAG 2 cut(s) 381, 857
DpnI GATC 5 cut(s) 190, 321, 598, 822, 951
DpnII GATC 5 cut(s) 188, 319, 596, 820, 949
DraI TTTAAA 1 cut(s) 555
DriI GACNNNNNGTC 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 846
Eam1105I GACNNNNNGTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 846
EciI GGCGGA 1 cut(s) 818
Ecl136II GAGCTC 2 cut(s) 535, 856
Eco130I CCWWGG 1 cut(s) 832
Eco147I AGGCCT 1 cut(s) 615
Eco24I GRGCYC 2 cut(s) 537, 858
Eco31I GGTCTC 3 cut(s) 140, 272, 474
Eco47I GGWCC 2 cut(s) 43, 101
Eco53kI GAGCTC 2 cut(s) 535, 856
Eco57I CTGAAG 2 cut(s) 188, 657
EcoICRI GAGCTC 2 cut(s) 535, 856
EcoO109I RGGNCCY 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 832
EcoT38I GRGCYC 2 cut(s) 537, 858
ErhI CCWWGG 1 cut(s) 832
FaeI CATG 4 cut(s) 154, 464, 693, 836
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 2 cut(s) 29, 101
FatI CATG 4 cut(s) 150, 460, 689, 832
FokI GGATG 3 cut(s) 812, 884, 974
FriOI GRGCYC 2 cut(s) 537, 858
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 3 cut(s) 199, 266, 330
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 3 cut(s) 61, 179, 615
HapII CCGG 3 cut(s) 62, 83, 110
HhaI GCGC 3 cut(s) 200, 267, 331
Hin1II CATG 4 cut(s) 154, 464, 693, 836
Hin6I GCGC 3 cut(s) 198, 265, 329
HinP1I GCGC 3 cut(s) 198, 265, 329
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HindIII AAGCTT 1 cut(s) 944
HinfI GANTC 5 cut(s) 20, 79, 571, 632, 905
HpaI GTTAAC 1 cut(s) 964
HpaII CCGG 3 cut(s) 62, 83, 110
HphI GGTGA 3 cut(s) 202, 307, 455
Hpy166II GTNNAC 3 cut(s) 141, 423, 964
Hpy188III TCNNGA 3 cut(s) 52, 237, 530
Hpy8I GTNNAC 3 cut(s) 141, 423, 964
Hpy99I CGWCG 2 cut(s) 101, 185
HpyAV CCTTC 3 cut(s) 212, 763, 992
HpyCH4III ACNGT 4 cut(s) 39, 101, 138, 794
HpyCH4IV ACGT 2 cut(s) 75, 549
HpyF10VI GCNNNNNNNGC 6 cut(s) 262, 367, 407, 665, 872, 993
HpyF3I CTNAG 2 cut(s) 381, 857
HpySE526I ACGT 2 cut(s) 75, 549
Hsp92II CATG 4 cut(s) 154, 464, 693, 836
HspAI GCGC 3 cut(s) 198, 265, 329
KflI GGGWCCC 1 cut(s) 43
KspAI GTTAAC 1 cut(s) 964
Kzo9I GATC 5 cut(s) 188, 319, 596, 820, 949
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 2 cut(s) 532, 540
LweI GCATC 5 cut(s) 370, 436, 853, 862, 996
MaeII ACGT 2 cut(s) 75, 549
MalI GATC 5 cut(s) 190, 321, 598, 822, 951
MboI GATC 5 cut(s) 188, 319, 596, 820, 949
MboII GAAGA 6 cut(s) 41, 163, 442, 554, 557, 863
MflI RGATCY 2 cut(s) 596, 820
MhlI GDGCHC 3 cut(s) 537, 812, 858
MluCI AATT 3 cut(s) 232, 346, 559
MlyI GAGTC 2 cut(s) 14, 641
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 6 cut(s) 204, 494, 605, 698, 756, 1001
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 7 cut(s) 269, 285, 336, 554, 654, 776, 963
MspI CCGG 3 cut(s) 62, 83, 110
MspR9I CCNGG 2 cut(s) 110, 608
MvaI CCWGG 1 cut(s) 608
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 6 cut(s) 262, 367, 407, 665, 872, 993
NciI CCSGG 1 cut(s) 110
NcoI CCATGG 1 cut(s) 832
NdeII GATC 5 cut(s) 188, 319, 596, 820, 949
NlaIII CATG 4 cut(s) 154, 464, 693, 836
NlaIV GGNNCC 2 cut(s) 44, 45
NsbI TGCGCA 1 cut(s) 330
PceI AGGCCT 1 cut(s) 615
PciSI GCTCTTC 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 3 cut(s) 79, 571, 905
Ple19I CGATCG 1 cut(s) 191
PleI GAGTC 2 cut(s) 14, 640
PpsI GAGTC 2 cut(s) 14, 640
PpuMI RGGWCCY 1 cut(s) 43
PshAI GACNNNNGTC 2 cut(s) 99, 525
Psp124BI GAGCTC 2 cut(s) 537, 858
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 2 cut(s) 44, 45
PspPI GGNCC 2 cut(s) 43, 101
PspPPI RGGWCCY 1 cut(s) 43
PsrI GAACNNNNNNTAC 2 cut(s) 385, 417
PsuI RGATCY 2 cut(s) 596, 820
PvuI CGATCG 1 cut(s) 191
RgaI GCGATCGC 1 cut(s) 191
Rsr2I CGGWCCG 1 cut(s) 101
RsrII CGGWCCG 1 cut(s) 101
SacI GAGCTC 2 cut(s) 537, 858
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 7 cut(s) 269, 285, 336, 554, 654, 776, 963
Sau3AI GATC 5 cut(s) 188, 319, 596, 820, 949
Sau96I GGNCC 2 cut(s) 43, 101
SchI GAGTC 2 cut(s) 14, 641
ScrFI CCNGG 2 cut(s) 110, 608
SduI GDGCHC 3 cut(s) 537, 812, 858
SfaAI GCGATCGC 1 cut(s) 191
SfaNI GCATC 5 cut(s) 370, 436, 853, 862, 996
SgfI GCGATCGC 1 cut(s) 191
SinI GGWCC 2 cut(s) 43, 101
SmlI CTYRAG 3 cut(s) 416, 488, 747
SmoI CTYRAG 3 cut(s) 416, 488, 747
Sse9I AATT 3 cut(s) 232, 346, 559
SseBI AGGCCT 1 cut(s) 615
SsiI CCGC 3 cut(s) 370, 410, 829
SstI GAGCTC 2 cut(s) 537, 858
StuI AGGCCT 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 108, 606
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 4 cut(s) 39, 101, 138, 794
TaiI ACGT 2 cut(s) 78, 552
TaqI TCGA 1 cut(s) 87
TasI AATT 3 cut(s) 232, 346, 559
TfiI GAWTC 3 cut(s) 79, 571, 905
Tru1I TTAA 7 cut(s) 269, 285, 336, 554, 654, 776, 963
Tru9I TTAA 7 cut(s) 269, 285, 336, 554, 654, 776, 963
TscAI CASTG 1 cut(s) 485
TspDTI ATGAA 4 cut(s) 167, 318, 528, 678
TspRI CASTG 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 43, 101
XapI RAATTY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.