MD04G1026300.v1.1

Voltage-gated hydrogen channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012015
Physical Location & Seq
Forward (+)
3050326 .. 3051081
756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1026300.v1.1.491

Sequence Viewer

Length: 756 bp
ATGAAAAACAAAGGGTCCTCATCATCTCAGATTCAACAACTACCTGGCCCTTTCACAACCACCACCTCATCATTCTCCATTGAATCCATTGAATCCTCAATCCAACGTCTCACAAGGAGCTGGCACAGAAGGCAACAATGGCAGAAGTTTCTCTGCAACAACTCCACACAGTTTCAAGACCTTAATGTTAATAGAGCACCATGGAGGACTCACTTGGCCAATTTCCTCGAGTCCAAACCAGTTCATACAATTTGTATTTCGTTGCTGATTACTGATCTCATTCTCACCATCCTCGAACTCTCCTCATCTTTATTTACCAAGTGCAACACAGCACCACCAAACAAGAGCAGTAGGACACAAGAAATCTGGTACCATTGGGTGGGGATTGCTATTCTCGCCCTGCTTTCGGCGAAGACGGTGGCTCAGGCTGTTGCGCTCGGCATGTCTACGTTTTTCAGGCGTCCCGGGTACATGGTCGACGGGGTTGTTTTGATGGGTGCGTTGGTGTTAGAAGAGTTTCTTGAGAGAAAAGGTGGCGGGCTGCTTGTTGTGGTCAGTTTGTGGCGTGTTGTTAGGGTGGTTGAGAGTGCTTTTGAGTTGAGTGATGAGGCCATTGAAGCACAGATTGCTAGTGTGGTGTGTCAGTTTGAGGTGCTGAGGGAGGAGAATAGGAGACTTGCTGAGACCATTGCTGAGAAGGACATGATTATCCAGAAGCTCCAGGAAGAATTAGAGCAATATGGTACTAGTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

28.36

Weight (kDa)

6.91

Isoelectric Point (pI)

56.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10800 AT1G10800
fragaria_vesca FvH4_4g12460
malus_domestica MD04G1026300.v1.1
prunus_persica Prupe.1G176000_v2.0.a1 Prupe.2G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0410771
rosa_laevigata RLG00000008397
rosa_multiflora Rmu_sc0009499.1_g000005
rosa_roxburghii Rroxscaffold_5G00355520
rosa_rugosa Rorug04G0110800 Rorug04G0110900 Rorug04G0110900 Rorug04G0111000
rosa_samantha Rh4AG163000 Rh4BG162200 Rh4CG175900 Rh4DG158700
rosa_wichuraiana Rw4G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 369
AccB7I CCANNNNNTGG 1 cut(s) 379
AccI GTMKAC 2 cut(s) 446, 477
AciI CCGC 1 cut(s) 537
AcoI YGGCCR 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 460
AfaI GTAC 3 cut(s) 371, 470, 745
AfiI CCNNNNNNNGG 2 cut(s) 379, 406
AgsI TTSAA 5 cut(s) 35, 83, 92, 176, 617
AhlI ACTAGT 1 cut(s) 746
AjnI CCWGG 2 cut(s) 43, 720
AluBI AGCT 2 cut(s) 120, 718
AluI AGCT 2 cut(s) 120, 718
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 3 cut(s) 113, 667, 677
Ama87I CYCGRG 2 cut(s) 227, 464
AoxI GGCC 3 cut(s) 46, 216, 609
ApeKI GCWGC 1 cut(s) 541
Asp700I GAANNNNTTC 1 cut(s) 516
Asp718I GGTACC 1 cut(s) 369
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 2 cut(s) 15, 47
AsuC2I CCSGG 2 cut(s) 465, 466
AsuHPI GGTGA 1 cut(s) 277
AvaI CYCGRG 2 cut(s) 227, 464
AvaII GGWCC 1 cut(s) 15
BalI TGGCCA 1 cut(s) 218
BanI GGYRCC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 199
BbvCI CCTCAGC 1 cut(s) 656
BbvI GCAGC 1 cut(s) 528
BccI CCATC 2 cut(s) 296, 487
BciT130I CCWGG 2 cut(s) 45, 722
BcnI CCSGG 2 cut(s) 465, 466
BcoDI GTCTC 3 cut(s) 113, 667, 677
BcuI ACTAGT 1 cut(s) 746
BfaI CTAG 2 cut(s) 630, 747
BisI GCNGC 1 cut(s) 542
BlsI GCNGC 1 cut(s) 543
Bme1390I CCNGG 4 cut(s) 45, 465, 466, 722
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 2 cut(s) 227, 464
BmgT120I GGNCC 2 cut(s) 15, 47
BmiI GGNNCC 2 cut(s) 16, 371
BmrFI CCNGG 4 cut(s) 45, 465, 466, 722
BpiI GAAGAC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 2 cut(s) 423, 656
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 2 cut(s) 465, 466
BsaHI GRCGYC 1 cut(s) 460
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 2 cut(s) 200, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 406
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 2 cut(s) 45, 722
BseDI CCNNGG 2 cut(s) 200, 464
BseGI GGATG 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 379, 406
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 5 cut(s) 41, 437, 647, 672, 684
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 2 cut(s) 292, 677
BseXI GCAGC 1 cut(s) 528
BshFI GGCC 3 cut(s) 48, 218, 611
BshNI GGYRCC 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 199
BsiHKCI CYCGRG 2 cut(s) 227, 464
BsiSI CCGG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 2 cut(s) 379, 406
BsmAI GTCTC 3 cut(s) 113, 667, 677
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 447
BsnI GGCC 3 cut(s) 48, 218, 611
Bso31I GGTCTC 1 cut(s) 677
BsoBI CYCGRG 2 cut(s) 227, 464
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 1 cut(s) 274
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 1 cut(s) 537
BspANI GGCC 3 cut(s) 48, 218, 611
BspCNI CTCAG 5 cut(s) 40, 436, 648, 673, 685
BspLI GGNNCC 2 cut(s) 16, 371
BspT107I GGYRCC 1 cut(s) 369
BspTNI GGTCTC 1 cut(s) 677
BsrDI GCAATG 1 cut(s) 687
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 200, 464
BssMI GATC 1 cut(s) 274
BssNI GRCGYC 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 45, 722
Bst4CI ACNGT 2 cut(s) 171, 418
Bst6I CTCTTC 1 cut(s) 507
BstACI GRCGYC 1 cut(s) 460
BstAPI GCANNNNNTGC 1 cut(s) 626
BstC8I GCNNGC 2 cut(s) 122, 539
BstDEI CTNAG 5 cut(s) 27, 423, 656, 681, 693
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 3 cut(s) 113, 667, 677
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 5 cut(s) 130, 139, 395, 617, 626
BstNI CCWGG 2 cut(s) 45, 722
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 4 cut(s) 43, 463, 464, 720
BstV1I GCAGC 1 cut(s) 528
BstV2I GAAGAC 1 cut(s) 419
BsuRI GGCC 3 cut(s) 48, 218, 611
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 288
Cac8I GCNNGC 2 cut(s) 122, 539
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 15, 47
Cfr9I CCCGGG 1 cut(s) 464
CseI GACGC 1 cut(s) 449
Csp6I GTAC 3 cut(s) 370, 469, 744
CviAII CATG 4 cut(s) 201, 442, 472, 703
CviJI RGCY 8 cut(s) 48, 120, 218, 422, 428, 541, 611, 718
CviKI_1 RGCY 8 cut(s) 48, 120, 218, 422, 428, 541, 611, 718
CviQI GTAC 3 cut(s) 370, 469, 744
DdeI CTNAG 5 cut(s) 27, 423, 656, 681, 693
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 200
Eco31I GGTCTC 1 cut(s) 677
Eco47I GGWCC 1 cut(s) 15
Eco88I CYCGRG 2 cut(s) 227, 464
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 2 cut(s) 43, 720
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 4 cut(s) 204, 445, 475, 706
FaiI YATR 6 cut(s) 202, 246, 443, 473, 704, 741
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FaqI GGGAC 1 cut(s) 447
FatI CATG 4 cut(s) 200, 441, 471, 702
FauI CCCGC 1 cut(s) 530
FblI GTMKAC 2 cut(s) 446, 477
Fnu4HI GCNGC 1 cut(s) 542
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 542
FspBI CTAG 2 cut(s) 630, 747
GlaI GCGC 1 cut(s) 435
GluI GCNGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 3 cut(s) 48, 218, 611
HapII CCGG 1 cut(s) 465
HgaI GACGC 1 cut(s) 449
HhaI GCGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 460
Hin1II CATG 4 cut(s) 204, 445, 475, 706
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 5 cut(s) 31, 83, 92, 208, 230
HpaII CCGG 1 cut(s) 465
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 2 cut(s) 447, 478
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 3 cut(s) 176, 521, 712
Hpy8I GTNNAC 2 cut(s) 447, 478
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 2 cut(s) 123, 691
HpyCH4III ACNGT 2 cut(s) 171, 418
HpyCH4IV ACGT 2 cut(s) 106, 449
HpyCH4V TGCA 2 cut(s) 156, 324
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 139, 395, 617, 626
HpyF3I CTNAG 5 cut(s) 27, 423, 656, 681, 693
HpySE526I ACGT 2 cut(s) 106, 449
Hsp92I GRCGYC 1 cut(s) 460
Hsp92II CATG 4 cut(s) 204, 445, 475, 706
HspAI GCGC 1 cut(s) 434
KpnI GGTACC 1 cut(s) 373
Kzo9I GATC 1 cut(s) 274
LmnI GCTCC 2 cut(s) 117, 723
Lsp1109I GCAGC 1 cut(s) 528
MaeI CTAG 2 cut(s) 630, 747
MaeII ACGT 2 cut(s) 106, 449
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 3 cut(s) 424, 524, 737
MhlI GDGCHC 1 cut(s) 199
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 3 cut(s) 220, 249, 728
MluNI TGGCCA 1 cut(s) 218
MlyI GAGTC 2 cut(s) 202, 239
MmeI TCCRAC 1 cut(s) 127
Mox20I TGGCCA 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 516
MscI TGGCCA 1 cut(s) 218
MseI TTAA 2 cut(s) 183, 189
Msp20I TGGCCA 1 cut(s) 218
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 4 cut(s) 45, 465, 466, 722
MvaI CCWGG 2 cut(s) 45, 722
MwoI GCNNNNNNNGC 5 cut(s) 130, 139, 395, 617, 626
NciI CCSGG 2 cut(s) 465, 466
NcoI CCATGG 1 cut(s) 200
NdeII GATC 1 cut(s) 274
NlaIII CATG 4 cut(s) 204, 445, 475, 706
NlaIV GGNNCC 2 cut(s) 16, 371
NmeAIII GCCGAG 1 cut(s) 417
NspI RCATGY 1 cut(s) 445
PaeR7I CTCGAG 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 3 cut(s) 31, 83, 92
PflMI CCANNNNNTGG 1 cut(s) 379
PfoI TCCNGGA 1 cut(s) 720
PkrI GCNGC 1 cut(s) 543
PleI GAGTC 2 cut(s) 202, 238
PpsI GAGTC 2 cut(s) 202, 238
PpuMI RGGWCCY 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 43, 720
PspGI CCWGG 2 cut(s) 43, 720
PspN4I GGNNCC 2 cut(s) 16, 371
PspPI GGNCC 2 cut(s) 15, 47
PspPPI RGGWCCY 1 cut(s) 15
PspXI VCTCGAGB 1 cut(s) 227
RsaI GTAC 3 cut(s) 371, 470, 745
RsaNI GTAC 3 cut(s) 370, 469, 744
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 183, 189
SatI GCNGC 1 cut(s) 542
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 2 cut(s) 15, 47
SchI GAGTC 2 cut(s) 202, 239
ScrFI CCNGG 4 cut(s) 45, 465, 466, 722
SduI GDGCHC 1 cut(s) 199
SetI ASST 9 cut(s) 46, 68, 109, 122, 183, 452, 535, 654, 720
Sfr274I CTCGAG 1 cut(s) 227
SinI GGWCC 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 227
SmaI CCCGGG 1 cut(s) 466
SmlI CTYRAG 2 cut(s) 227, 521
SmoI CTYRAG 2 cut(s) 227, 521
SpeI ACTAGT 1 cut(s) 746
Sse9I AATT 3 cut(s) 220, 249, 728
SsiI CCGC 1 cut(s) 537
SspMI CTAG 2 cut(s) 630, 747
StyD4I CCNGG 4 cut(s) 43, 463, 464, 720
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 2 cut(s) 171, 418
TaiI ACGT 2 cut(s) 109, 452
TaqI TCGA 3 cut(s) 228, 294, 477
TasI AATT 3 cut(s) 220, 249, 728
TfiI GAWTC 3 cut(s) 31, 83, 92
Tru1I TTAA 2 cut(s) 183, 189
Tru9I TTAA 2 cut(s) 183, 189
TseI GCWGC 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 17, 233
TspMI CCCGGG 1 cut(s) 464
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 15
XceI RCATGY 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 227
XmaI CCCGGG 1 cut(s) 464
XmiI GTMKAC 2 cut(s) 446, 477
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 2 cut(s) 630, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.