Rh4DG158700

Voltage-gated hydrogen channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012015
Physical Location & Seq
Reverse (-)
31629598 .. 31635559
5962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG158700.1

Sequence Viewer

Length: 813 bp
ATGGCATTCATCGTGACCCCAAAGCATAAGGACGTCAAGCTTGCACTTCACGTATCAATATCATTCACCAAGAATGGGTCCTCCTCTCAAATCCAGCAACAGAGACCTTCCACAGCAAACTCATTCTCTATAGAGTCTGTCGAATCCTCGATACGAAGTCTGTTAAGGAGCTGGTACAGACGGCAGAAATGGCAAGTTTTATTCAACTCCACACCACAACAAGAACTTGATAGAGCTCCATGGAGAACCCATCTGGCCAGTTTCCTTGAATCCACACCAGTTCATGCAGTTTCTCTCGGTCTCCTCATTATCGATCTCATCTTCACCATCCTTGAACTCTCCTCATCTTTACTTGCTACCAGTTGCAGGCCAAACGAGAACCACAAAGCTACAGAAGAAATCTGGTACCACTGGTTTGGCGTTGCTATTCTTACTCTGCTTTCGGCAAAGACTATTGCCCTTGCTGTGGGGCTCGGCACCACATTCTTTAGGCGTCCGGGTTATGTCATTGATGGGATCGTTTTGATGGGCGCGTTGGGGTTAGAAGTGTTTTTAGACAAGATAGGGGGTGGGTTGCTTGTTGTGGTCAGTTTGTGGCGAGTTGTTAGGGTAGTAGAAAGTGCATTTGAGCTGAGTGATGAGGCCATTGAAGCACAGATTGAAGGGCTGGTGTGCCAGTTTGAGATGTTGAGGGAAGAGAATAGGAGACTGTTAGAAACCATTGGTGAGAAAGATCAGATTATCCAGAAGCTCCAGGAAGATTCAGATCAATGTACATGTTGCTCCAGTCAAAAATCTTTATCTTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

30.34

Weight (kDa)

5.92

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10800 AT1G10800
fragaria_vesca FvH4_4g12460
malus_domestica MD04G1026300.v1.1
prunus_persica Prupe.1G176000_v2.0.a1 Prupe.2G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0410771
rosa_laevigata RLG00000008397
rosa_multiflora Rmu_sc0009499.1_g000005
rosa_roxburghii Rroxscaffold_5G00355520
rosa_rugosa Rorug04G0110800 Rorug04G0110900 Rorug04G0110900 Rorug04G0111000
rosa_samantha Rh4AG163000 Rh4BG162200 Rh4CG175900 Rh4DG158700
rosa_wichuraiana Rw4G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 2 cut(s) 405, 476
AccII CGCG 1 cut(s) 533
AclWI GGATC 1 cut(s) 524
AcoI YGGCCR 1 cut(s) 255
AcyI GRCGYC 2 cut(s) 33, 493
AfaI GTAC 3 cut(s) 176, 407, 775
AfiI CCNNNNNNNGG 3 cut(s) 75, 366, 466
AflIII ACRYGT 1 cut(s) 776
AgsI TTSAA 5 cut(s) 205, 269, 335, 650, 662
AjnI CCWGG 1 cut(s) 753
AluBI AGCT 6 cut(s) 40, 171, 236, 389, 631, 751
AluI AGCT 6 cut(s) 40, 171, 236, 389, 631, 751
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 3 cut(s) 97, 305, 700
AlwI GGATC 1 cut(s) 524
AoxI GGCC 3 cut(s) 255, 368, 642
Asp718I GGTACC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 3 cut(s) 58, 316, 737
AvaII GGWCC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 2 cut(s) 405, 476
BanII GRGCYC 2 cut(s) 238, 474
Bbv12I GWGCWC 1 cut(s) 238
BccI CCATC 4 cut(s) 258, 335, 506, 520
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 1 cut(s) 755
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 3 cut(s) 97, 305, 700
BfmI CTRYAG 2 cut(s) 129, 390
Bme1390I CCNGG 2 cut(s) 498, 755
Bme18I GGWCC 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 3 cut(s) 79, 407, 478
BmrFI CCNGG 2 cut(s) 498, 755
BpmI CTGGAG 2 cut(s) 737, 769
BpuMI CCSGG 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 2 cut(s) 33, 493
BsaI GGTCTC 2 cut(s) 97, 305
BsaJI CCNNGG 1 cut(s) 239
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 366, 466
Bse1I ACTGG 6 cut(s) 258, 278, 360, 416, 676, 786
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 1 cut(s) 755
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 239
BseGI GGATG 1 cut(s) 327
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 3 cut(s) 75, 366, 466
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 6 cut(s) 258, 278, 360, 416, 676, 786
BseRI GAGGAG 3 cut(s) 73, 293, 331
Bsh1236I CGCG 1 cut(s) 533
BshFI GGCC 3 cut(s) 257, 370, 644
BshNI GGYRCC 2 cut(s) 405, 476
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 75, 366, 466
BsmAI GTCTC 3 cut(s) 97, 305, 700
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 3 cut(s) 257, 370, 644
Bso31I GGTCTC 2 cut(s) 97, 305
Bsp1286I GDGCHC 2 cut(s) 238, 474
Bsp1407I TGTACA 1 cut(s) 773
Bsp143I GATC 4 cut(s) 313, 516, 733, 766
Bsp19I CCATGG 1 cut(s) 239
BspANI GGCC 3 cut(s) 257, 370, 644
BspCNI CTCAG 1 cut(s) 624
BspDI ATCGAT 1 cut(s) 312
BspFNI CGCG 1 cut(s) 533
BspLI GGNNCC 3 cut(s) 79, 407, 478
BspPI GGATC 1 cut(s) 524
BspT107I GGYRCC 2 cut(s) 405, 476
BspTNI GGTCTC 2 cut(s) 97, 305
BsrGI TGTACA 1 cut(s) 773
BsrI ACTGG 6 cut(s) 258, 278, 360, 416, 676, 786
BssECI CCNNGG 1 cut(s) 239
BssMI GATC 4 cut(s) 313, 516, 733, 766
BssNI GRCGYC 2 cut(s) 33, 493
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 1 cut(s) 711
Bst6I CTCTTC 1 cut(s) 690
BstACI GRCGYC 2 cut(s) 33, 493
BstAUI TGTACA 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 2 cut(s) 42, 368
BstDEI CTNAG 1 cut(s) 632
BstDSI CCRYGG 1 cut(s) 239
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 533
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 4 cut(s) 316, 519, 736, 769
BstMAI GTCTC 3 cut(s) 97, 305, 700
BstMBI GATC 4 cut(s) 313, 516, 733, 766
BstMWI GCNNNNNNNGC 2 cut(s) 190, 650
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 496, 753
BstSFI CTRYAG 2 cut(s) 129, 390
BstUI CGCG 1 cut(s) 533
BstXI CCANNNNNNTGG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 3 cut(s) 257, 370, 644
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 239
BtsCI GGATG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 42, 368
CfoI GCGC 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 312
CseI GACGC 1 cut(s) 482
Csp6I GTAC 3 cut(s) 175, 406, 774
CviAII CATG 3 cut(s) 240, 284, 777
CviQI GTAC 3 cut(s) 175, 406, 774
DdeI CTNAG 1 cut(s) 632
DpnI GATC 4 cut(s) 315, 518, 735, 768
DpnII GATC 4 cut(s) 313, 516, 733, 766
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 239
Eco24I GRGCYC 2 cut(s) 238, 474
Eco31I GGTCTC 2 cut(s) 97, 305
Eco47I GGWCC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 1 cut(s) 239
EcoT38I GRGCYC 2 cut(s) 238, 474
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 3 cut(s) 243, 287, 780
FaiI YATR 7 cut(s) 27, 131, 241, 285, 504, 778, 807
FatI CATG 3 cut(s) 239, 283, 776
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 2 cut(s) 238, 474
GlaI GCGC 1 cut(s) 532
GsuI CTGGAG 2 cut(s) 737, 769
HaeIII GGCC 3 cut(s) 257, 370, 644
HapII CCGG 1 cut(s) 497
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 2 cut(s) 33, 493
Hin1II CATG 3 cut(s) 243, 287, 780
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 4 cut(s) 134, 143, 269, 761
HpaII CCGG 1 cut(s) 497
HphI GGTGA 3 cut(s) 58, 316, 737
Hpy188I TCNGA 2 cut(s) 738, 766
Hpy188III TCNNGA 2 cut(s) 13, 745
HpyAV CCTTC 2 cut(s) 117, 656
HpyCH4III ACNGT 1 cut(s) 711
HpyCH4IV ACGT 2 cut(s) 33, 51
HpyCH4V TGCA 4 cut(s) 44, 287, 366, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 650
HpyF3I CTNAG 1 cut(s) 632
HpySE526I ACGT 2 cut(s) 33, 51
Hsp92I GRCGYC 2 cut(s) 33, 493
Hsp92II CATG 3 cut(s) 243, 287, 780
HspAI GCGC 1 cut(s) 531
KpnI GGTACC 1 cut(s) 409
Kzo9I GATC 4 cut(s) 313, 516, 733, 766
LmnI GCTCC 4 cut(s) 168, 241, 756, 788
MaeII ACGT 2 cut(s) 33, 51
MaeIII GTNAC 1 cut(s) 13
MalI GATC 4 cut(s) 315, 518, 735, 768
MboI GATC 4 cut(s) 313, 516, 733, 766
MboII GAAGA 4 cut(s) 313, 407, 707, 770
MhlI GDGCHC 2 cut(s) 238, 474
MlsI TGGCCA 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 7 cut(s) 91, 94, 157, 314, 352, 634, 684
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 2 cut(s) 498, 755
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 755
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 2 cut(s) 190, 650
NciI CCSGG 1 cut(s) 498
NcoI CCATGG 1 cut(s) 239
NdeII GATC 4 cut(s) 313, 516, 733, 766
NlaIII CATG 3 cut(s) 243, 287, 780
NlaIV GGNNCC 3 cut(s) 79, 407, 478
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 780
PciI ACATGT 1 cut(s) 776
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 3 cut(s) 143, 269, 761
PfoI TCCNGGA 1 cut(s) 753
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 78
PscI ACATGT 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 753
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 3 cut(s) 79, 407, 478
PspPI GGNCC 1 cut(s) 78
PspPPI RGGWCCY 1 cut(s) 78
RsaI GTAC 3 cut(s) 176, 407, 775
RsaNI GTAC 3 cut(s) 175, 406, 774
SacI GAGCTC 1 cut(s) 238
SaqAI TTAA 1 cut(s) 164
Sau3AI GATC 4 cut(s) 313, 516, 733, 766
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 498, 755
SduI GDGCHC 2 cut(s) 238, 474
SetI ASST 9 cut(s) 36, 42, 54, 109, 173, 238, 391, 633, 753
SfcI CTRYAG 2 cut(s) 129, 390
SinI GGWCC 1 cut(s) 78
SstI GAGCTC 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 496, 753
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 711
TaiI ACGT 2 cut(s) 36, 54
TaqI TCGA 3 cut(s) 141, 149, 312
TaqII GACCGA 1 cut(s) 287
TatI WGTACW 1 cut(s) 773
TfiI GAWTC 3 cut(s) 143, 269, 761
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 1 cut(s) 416
TseFI GTSAC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 1 cut(s) 272
TspRI CASTG 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 78
XceI RCATGY 1 cut(s) 780
ZraI GACGTC 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.