Prupe.2G050500_v2.0.a1

Voltage-gated hydrogen channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012015
Physical Location & Seq
Reverse (-)
5857492 .. 5858256
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G050500.1

Sequence Viewer

Length: 765 bp
ATGAAAATTGCCTCATCTCAGATTCAAATTCAACAACCTGGGCCTCCCACAACCACCACCACCACCACCACCAACTCATTCTCTGTTGAATCCACTGAAGCCTCAATCCAGAGCCTGACAAGGAGCTGGCATAGAAGGCAACATTGGCACAGGTTTTTTTGGTTTGATAATAATAATTCCACACAATTTCAAGACCTAAATTTAACACCATGGAGAACCCACTTGGCCAATTTTCTAGAGTCTGCACCAATCCATGTGATTTCTATCTCCCTCCTCATTGTAGATCTCATCCTCACCATCCTTGAACTCTCCTCATCTTTGCTAACCAAGTGCAACAACTCACCAAACAAGAACAAAAAAACCACACCAGAAATTTGGTACCATTGGGTGGGCATTGCTATCCTCGCCCTGCTTTCGGCAAAGACGATGGCTCAAGCTGTGGGGCTTGGCATGCGTACGTTTCTCCGGCGGCGTCCCGGTTACGTCATTGACGGCGTCGTTTTGATGGGTGCGCTGGTTTTAGAAGCTTTTTTCGAGAGGAAGGGAGGGGGGCTGCTTGTTGTGGTGAGTTTGTGGCGTGTTGTTAGGGTTGTAGAGAGTGCTTTTGAGTTGAGCGATGAGGCCATTGAAGCGCAGATTCAAAGTGTTGTTTGCCAGTTTGAGATGCTGAAGGAAGAGAACAGGAGACTCGCAGAGACCATTAGTGAGCAGGACAAGATTATCGAGAAGCTCCAGGAAGATTTAGATCAATATTCTACTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.92

Weight (kDa)

6.6

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10800 AT1G10800
fragaria_vesca FvH4_4g12460
malus_domestica MD04G1026300.v1.1
prunus_persica Prupe.1G176000_v2.0.a1 Prupe.2G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0410771
rosa_laevigata RLG00000008397
rosa_multiflora Rmu_sc0009499.1_g000005
rosa_roxburghii Rroxscaffold_5G00355520
rosa_rugosa Rorug04G0110800 Rorug04G0110900 Rorug04G0110900 Rorug04G0111000
rosa_samantha Rh4AG163000 Rh4BG162200 Rh4CG175900 Rh4DG158700
rosa_wichuraiana Rw4G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 378
AccB1I GGYRCC 1 cut(s) 378
AccB7I CCANNNNNTGG 1 cut(s) 388
AciI CCGC 1 cut(s) 469
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 3 cut(s) 27, 199, 372
AcuI CTGAAG 2 cut(s) 117, 689
AcyI GRCGYC 2 cut(s) 472, 495
AfaI GTAC 2 cut(s) 380, 457
AfiI CCNNNNNNNGG 2 cut(s) 388, 415
AgsI TTSAA 7 cut(s) 26, 32, 89, 191, 305, 629, 641
AjnI CCWGG 2 cut(s) 37, 732
AjuI GAANNNNNNNTTGG 2 cut(s) 127, 159
AluBI AGCT 4 cut(s) 126, 437, 527, 730
AluI AGCT 4 cut(s) 126, 437, 527, 730
Alw26I GTCTC 2 cut(s) 679, 689
AlwNI CAGNNNCTG 1 cut(s) 115
AoxI GGCC 3 cut(s) 41, 225, 621
ApeKI GCWGC 1 cut(s) 553
ApoI RAATTY 3 cut(s) 27, 199, 372
Asp718I GGTACC 1 cut(s) 378
AspLEI GCGC 2 cut(s) 514, 634
AspS9I GGNCC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 3 cut(s) 286, 333, 577
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 540
BccI CCATC 3 cut(s) 305, 421, 499
BceAI ACGGC 1 cut(s) 508
BciT130I CCWGG 2 cut(s) 39, 734
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 2 cut(s) 679, 689
BfaI CTAG 2 cut(s) 236, 763
BglII AGATCT 1 cut(s) 283
BisI GCNGC 2 cut(s) 470, 554
BlsI GCNGC 2 cut(s) 471, 555
Bme1390I CCNGG 3 cut(s) 39, 477, 734
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 3 cut(s) 39, 477, 734
BmsI GCATC 1 cut(s) 654
BpmI CTGGAG 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 477
BsaBI GATNNNNATC 2 cut(s) 263, 744
BsaHI GRCGYC 2 cut(s) 472, 495
BsaI GGTCTC 1 cut(s) 689
BsaJI CCNNGG 2 cut(s) 38, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 415
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 393
Bse8I GATNNNNATC 2 cut(s) 263, 744
BseBI CCWGG 2 cut(s) 39, 734
BseDI CCNNGG 2 cut(s) 38, 209
BseGI GGATG 2 cut(s) 288, 297
BseJI GATNNNNATC 2 cut(s) 263, 744
BseLI CCNNNNNNNGG 2 cut(s) 388, 415
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 655
BseRI GAGGAG 2 cut(s) 263, 301
BseXI GCAGC 1 cut(s) 540
BsgI GTGCAG 1 cut(s) 228
BshFI GGCC 3 cut(s) 43, 227, 623
BshNI GGYRCC 1 cut(s) 378
BsiSI CCGG 2 cut(s) 466, 477
BsiWI CGTACG 1 cut(s) 455
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 2 cut(s) 388, 415
BsmAI GTCTC 2 cut(s) 679, 689
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 3 cut(s) 43, 227, 623
Bso31I GGTCTC 1 cut(s) 689
Bsp143I GATC 2 cut(s) 283, 745
Bsp19I CCATGG 1 cut(s) 209
BspACI CCGC 1 cut(s) 469
BspANI GGCC 3 cut(s) 43, 227, 623
BspCNI CTCAG 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 393
BsrI ACTGG 1 cut(s) 655
BssECI CCNNGG 2 cut(s) 38, 209
BssMI GATC 2 cut(s) 283, 745
BssNI GRCGYC 2 cut(s) 472, 495
BssT1I CCWWGG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 39, 734
Bst6I CTCTTC 1 cut(s) 669
BstACI GRCGYC 2 cut(s) 472, 495
BstC8I GCNNGC 2 cut(s) 128, 452
BstDEI CTNAG 1 cut(s) 18
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 288, 297
BstHHI GCGC 2 cut(s) 514, 634
BstKTI GATC 2 cut(s) 286, 748
BstMAI GTCTC 2 cut(s) 679, 689
BstMBI GATC 2 cut(s) 283, 745
BstMWI GCNNNNNNNGC 5 cut(s) 136, 145, 404, 451, 629
BstNI CCWGG 2 cut(s) 39, 734
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 3 cut(s) 37, 475, 732
BstV1I GCAGC 1 cut(s) 540
BstX2I RGATCY 1 cut(s) 283
BstXI CCANNNNNNTGG 1 cut(s) 375
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 3 cut(s) 43, 227, 623
BtgI CCRYGG 1 cut(s) 209
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 2 cut(s) 288, 297
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 2 cut(s) 128, 452
CaiI CAGNNNCTG 1 cut(s) 115
CfoI GCGC 2 cut(s) 514, 634
Cfr13I GGNCC 1 cut(s) 41
CseI GACGC 2 cut(s) 461, 484
Csp6I GTAC 2 cut(s) 379, 456
CviAII CATG 3 cut(s) 210, 254, 451
CviQI GTAC 2 cut(s) 379, 456
DdeI CTNAG 1 cut(s) 18
DpnI GATC 2 cut(s) 285, 747
DpnII GATC 2 cut(s) 283, 745
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 669
EarI CTCTTC 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 689
Eco57I CTGAAG 2 cut(s) 117, 689
EcoRII CCWGG 2 cut(s) 37, 732
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 3 cut(s) 213, 257, 454
FaiI YATR 4 cut(s) 132, 211, 255, 452
FaqI GGGAC 1 cut(s) 459
FatI CATG 3 cut(s) 209, 253, 450
Fnu4HI GCNGC 2 cut(s) 470, 554
FokI GGATG 2 cut(s) 275, 284
Fsp4HI GCNGC 2 cut(s) 470, 554
FspBI CTAG 2 cut(s) 236, 763
GlaI GCGC 2 cut(s) 513, 633
GluI GCNGC 2 cut(s) 470, 554
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 3 cut(s) 43, 227, 623
HapII CCGG 2 cut(s) 466, 477
HgaI GACGC 2 cut(s) 461, 484
HhaI GCGC 2 cut(s) 514, 634
Hin1I GRCGYC 2 cut(s) 472, 495
Hin1II CATG 3 cut(s) 213, 257, 454
Hin6I GCGC 2 cut(s) 512, 632
HinP1I GCGC 2 cut(s) 512, 632
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 5 cut(s) 22, 89, 239, 637, 687
HpaII CCGG 2 cut(s) 466, 477
HphI GGTGA 3 cut(s) 286, 333, 577
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 5 cut(s) 109, 191, 236, 535, 724
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 3 cut(s) 129, 535, 664
HpyCH4IV ACGT 2 cut(s) 458, 483
HpyCH4V TGCA 2 cut(s) 245, 333
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 145, 404, 451, 629
HpyF3I CTNAG 1 cut(s) 18
HpySE526I ACGT 2 cut(s) 458, 483
Hsp92I GRCGYC 2 cut(s) 472, 495
Hsp92II CATG 3 cut(s) 213, 257, 454
HspAI GCGC 2 cut(s) 512, 632
KpnI GGTACC 1 cut(s) 382
Kzo9I GATC 2 cut(s) 283, 745
LmnI GCTCC 2 cut(s) 123, 735
Lsp1109I GCAGC 1 cut(s) 540
LweI GCATC 1 cut(s) 654
MaeI CTAG 2 cut(s) 236, 763
MaeII ACGT 2 cut(s) 458, 483
MaeIII GTNAC 1 cut(s) 479
MalI GATC 2 cut(s) 285, 747
MboI GATC 2 cut(s) 283, 745
MboII GAAGA 2 cut(s) 686, 749
MflI RGATCY 1 cut(s) 283
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 7 cut(s) 6, 27, 175, 185, 199, 229, 372
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 2 cut(s) 248, 681
Mox20I TGGCCA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 227
MseI TTAA 1 cut(s) 203
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 2 cut(s) 466, 477
MspR9I CCNGG 3 cut(s) 39, 477, 734
MvaI CCWGG 2 cut(s) 39, 734
MwoI GCNNNNNNNGC 5 cut(s) 136, 145, 404, 451, 629
NciI CCSGG 1 cut(s) 477
NcoI CCATGG 1 cut(s) 209
NdeII GATC 2 cut(s) 283, 745
NlaIII CATG 3 cut(s) 213, 257, 454
NlaIV GGNNCC 1 cut(s) 380
NspI RCATGY 1 cut(s) 454
PaeI GCATGC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PfeI GAWTC 3 cut(s) 22, 89, 637
Pfl23II CGTACG 1 cut(s) 455
PflFI GACNNNGTC 1 cut(s) 494
PflMI CCANNNNNTGG 1 cut(s) 388
PfoI TCCNGGA 1 cut(s) 732
PkrI GCNGC 2 cut(s) 471, 555
PleI GAGTC 2 cut(s) 247, 681
PpsI GAGTC 2 cut(s) 247, 681
Psp6I CCWGG 2 cut(s) 37, 732
PspGI CCWGG 2 cut(s) 37, 732
PspLI CGTACG 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 1 cut(s) 283
PsyI GACNNNGTC 1 cut(s) 494
RsaI GTAC 2 cut(s) 380, 457
RsaNI GTAC 2 cut(s) 379, 456
SaqAI TTAA 1 cut(s) 203
SatI GCNGC 2 cut(s) 470, 554
Sau3AI GATC 2 cut(s) 283, 745
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 2 cut(s) 248, 681
ScrFI CCNGG 3 cut(s) 39, 477, 734
SetI ASST 9 cut(s) 40, 128, 155, 198, 439, 461, 486, 529, 732
SfaNI GCATC 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 432
SmoI CTYRAG 1 cut(s) 432
SphI GCATGC 1 cut(s) 454
Sse9I AATT 7 cut(s) 6, 27, 175, 185, 199, 229, 372
SsiI CCGC 1 cut(s) 469
SspI AATATT 1 cut(s) 752
SspMI CTAG 2 cut(s) 236, 763
StyD4I CCNGG 3 cut(s) 37, 475, 732
StyI CCWWGG 1 cut(s) 209
TaiI ACGT 2 cut(s) 461, 486
TaqI TCGA 2 cut(s) 534, 723
TasI AATT 7 cut(s) 6, 27, 175, 185, 199, 229, 372
TauI GCSGC 1 cut(s) 472
TfiI GAWTC 3 cut(s) 22, 89, 637
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TscAI CASTG 1 cut(s) 100
TseI GCWGC 1 cut(s) 553
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 100
Tth111I GACNNNGTC 1 cut(s) 494
Van91I CCANNNNNTGG 1 cut(s) 388
XapI RAATTY 3 cut(s) 27, 199, 372
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 1 cut(s) 454
XspI CTAG 2 cut(s) 236, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.