Rh4BG162200

Voltage-gated hydrogen channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012015
Physical Location & Seq
Reverse (-)
29187604 .. 29188499
896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG162200.1

Sequence Viewer

Length: 810 bp
ATGGCATTCAACGTGACCCCAAAGCATAAGAACGTCAAGCTTGCACTTCACGTATCAATATCATTCACCAAGAATGGGTCCTCCTCTCAAATCCAGCAACAGAGACCTTCCACAGCAAACTGCTCTATAGAGTCTGTCGAATCCTCGATACGAAGTCTGTTAAGGAGCTGGTACAGACGGCAGAAATGGCAAGTTTTATTCAACTCCACACCACCACAAGAACTTGATAGAGCTCCATGGAGAACCCATCTGGCCAGTTTCCTTGAATCCACACCAGTTCATGCAGTTTCTCTCGGTCTCCTCATTATCGATCTCATCTTCACCATCCTTGAACTCTCCTCATCTTTACTTGCTACCAGTTGCAGGCCAAACGAGAACCACAAAGCTACAGAAGAAATCTGGTACCACTGGGTTGGCGTTGCTATTCTTACTCTGCTTTCAGCAAAGACTATTGCCCTTGCTGTGGGGCTCGGCACCACATTCTTTAGGCGTCCGGGTTATGTCATTGATGGGATCGTTTTGATGGGCGCGTTGGGGTTAGAAGTGTTTTTAGACAAGATAGGGGGTGGGTTGCTTGTTGTGGTCAGTTTGTGGCGAGTTGTTAGGGTAGTAGAAAGTGCATTTGAGCTGAGTGATGAGGCCATTGAAGCACAGATTGAAGGGCTTGTGTGCCAGTTTGAGATGTTGAGGGAAGAGAATAGGAGACTGTTAGAAATCATTGCTGAGAAAGATCAGATTATCCAGAAGCTGCAGGAAGATTCAGATCAATGTACATGTGGCTCCAGTCGAAAATCTTTACCTTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

269

Amino Acids

30.15

Weight (kDa)

6.33

Isoelectric Point (pI)

53.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10800 AT1G10800
fragaria_vesca FvH4_4g12460
malus_domestica MD04G1026300.v1.1
prunus_persica Prupe.1G176000_v2.0.a1 Prupe.2G050500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0410771
rosa_laevigata RLG00000008397
rosa_multiflora Rmu_sc0009499.1_g000005
rosa_roxburghii Rroxscaffold_5G00355520
rosa_rugosa Rorug04G0110800 Rorug04G0110900 Rorug04G0110900 Rorug04G0111000
rosa_samantha Rh4AG163000 Rh4BG162200 Rh4CG175900 Rh4DG158700
rosa_wichuraiana Rw4G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 2 cut(s) 402, 473
AccII CGCG 1 cut(s) 530
AclWI GGATC 1 cut(s) 521
AcoI YGGCCR 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 490
AfaI GTAC 3 cut(s) 173, 404, 772
AfiI CCNNNNNNNGG 3 cut(s) 75, 363, 463
AflIII ACRYGT 1 cut(s) 773
AgsI TTSAA 6 cut(s) 10, 202, 266, 332, 647, 659
AluBI AGCT 6 cut(s) 40, 168, 233, 386, 628, 748
AluI AGCT 6 cut(s) 40, 168, 233, 386, 628, 748
Alw21I GWGCWC 1 cut(s) 235
Alw26I GTCTC 3 cut(s) 97, 302, 697
AlwI GGATC 1 cut(s) 521
AlwNI CAGNNNCTG 1 cut(s) 748
AoxI GGCC 3 cut(s) 252, 365, 639
ApeKI GCWGC 1 cut(s) 748
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 58, 313
AvaII GGWCC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 2 cut(s) 402, 473
BanII GRGCYC 2 cut(s) 235, 471
Bbv12I GWGCWC 1 cut(s) 235
BbvI GCAGC 1 cut(s) 735
BccI CCATC 4 cut(s) 255, 332, 503, 517
BceAI ACGGC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 3 cut(s) 97, 302, 697
BfmI CTRYAG 3 cut(s) 126, 387, 749
BisI GCNGC 1 cut(s) 749
BlsI GCNGC 1 cut(s) 750
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 4 cut(s) 79, 404, 475, 781
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 766
BpuMI CCSGG 1 cut(s) 495
Bsa29I ATCGAT 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 490
BsaI GGTCTC 2 cut(s) 97, 302
BsaJI CCNNGG 1 cut(s) 236
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 363, 463
Bse1I ACTGG 6 cut(s) 255, 275, 357, 413, 673, 783
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 762
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 1 cut(s) 324
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 3 cut(s) 75, 363, 463
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 620, 714
BseNI ACTGG 6 cut(s) 255, 275, 357, 413, 673, 783
BseRI GAGGAG 3 cut(s) 73, 290, 328
BseXI GCAGC 1 cut(s) 735
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 3 cut(s) 254, 367, 641
BshNI GGYRCC 2 cut(s) 402, 473
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 235
BsiSI CCGG 1 cut(s) 494
BslI CCNNNNNNNGG 3 cut(s) 75, 363, 463
BsmAI GTCTC 3 cut(s) 97, 302, 697
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 3 cut(s) 254, 367, 641
Bso31I GGTCTC 2 cut(s) 97, 302
Bsp1286I GDGCHC 2 cut(s) 235, 471
Bsp1407I TGTACA 1 cut(s) 770
Bsp143I GATC 4 cut(s) 310, 513, 730, 763
Bsp19I CCATGG 1 cut(s) 236
BspANI GGCC 3 cut(s) 254, 367, 641
BspCNI CTCAG 2 cut(s) 621, 715
BspDI ATCGAT 1 cut(s) 309
BspFNI CGCG 1 cut(s) 530
BspLI GGNNCC 4 cut(s) 79, 404, 475, 781
BspMAI CTGCAG 1 cut(s) 753
BspPI GGATC 1 cut(s) 521
BspT107I GGYRCC 2 cut(s) 402, 473
BspTNI GGTCTC 2 cut(s) 97, 302
BsrDI GCAATG 1 cut(s) 717
BsrGI TGTACA 1 cut(s) 770
BsrI ACTGG 6 cut(s) 255, 275, 357, 413, 673, 783
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 4 cut(s) 310, 513, 730, 763
BssNI GRCGYC 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 708
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 490
BstAUI TGTACA 1 cut(s) 770
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 2 cut(s) 42, 365
BstDEI CTNAG 2 cut(s) 629, 723
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 530
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 4 cut(s) 313, 516, 733, 766
BstMAI GTCTC 3 cut(s) 97, 302, 697
BstMBI GATC 4 cut(s) 310, 513, 730, 763
BstMWI GCNNNNNNNGC 2 cut(s) 187, 647
BstNSI RCATGY 1 cut(s) 777
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 3 cut(s) 126, 387, 749
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 1 cut(s) 735
BstXI CCANNNNNNTGG 1 cut(s) 413
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 3 cut(s) 254, 367, 641
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 236
BtsCI GGATG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 42, 365
CaiI CAGNNNCTG 1 cut(s) 748
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 309
CseI GACGC 1 cut(s) 479
Csp6I GTAC 3 cut(s) 172, 403, 771
CviAII CATG 3 cut(s) 237, 281, 774
CviQI GTAC 3 cut(s) 172, 403, 771
DdeI CTNAG 2 cut(s) 629, 723
DpnI GATC 4 cut(s) 312, 515, 732, 765
DpnII GATC 4 cut(s) 310, 513, 730, 763
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 2 cut(s) 235, 471
Eco31I GGTCTC 2 cut(s) 97, 302
Eco47I GGWCC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 2 cut(s) 235, 471
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 3 cut(s) 240, 284, 777
FaiI YATR 7 cut(s) 27, 128, 238, 282, 501, 775, 804
FatI CATG 3 cut(s) 236, 280, 773
Fnu4HI GCNGC 1 cut(s) 749
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 2 cut(s) 235, 471
Fsp4HI GCNGC 1 cut(s) 749
GlaI GCGC 1 cut(s) 529
GluI GCNGC 1 cut(s) 749
GsuI CTGGAG 1 cut(s) 766
HaeIII GGCC 3 cut(s) 254, 367, 641
HapII CCGG 1 cut(s) 494
HgaI GACGC 1 cut(s) 479
HhaI GCGC 1 cut(s) 530
Hin1I GRCGYC 1 cut(s) 490
Hin1II CATG 3 cut(s) 240, 284, 777
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 4 cut(s) 131, 140, 266, 758
HpaII CCGG 1 cut(s) 494
HphI GGTGA 2 cut(s) 58, 313
Hpy188I TCNGA 2 cut(s) 735, 763
Hpy188III TCNNGA 1 cut(s) 742
HpyAV CCTTC 2 cut(s) 117, 653
HpyCH4III ACNGT 1 cut(s) 708
HpyCH4IV ACGT 3 cut(s) 12, 33, 51
HpyCH4V TGCA 5 cut(s) 44, 284, 363, 620, 751
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 647
HpyF3I CTNAG 2 cut(s) 629, 723
HpySE526I ACGT 3 cut(s) 12, 33, 51
Hsp92I GRCGYC 1 cut(s) 490
Hsp92II CATG 3 cut(s) 240, 284, 777
HspAI GCGC 1 cut(s) 528
KpnI GGTACC 1 cut(s) 406
Kzo9I GATC 4 cut(s) 310, 513, 730, 763
LmnI GCTCC 3 cut(s) 165, 238, 785
Lsp1109I GCAGC 1 cut(s) 735
MaeII ACGT 3 cut(s) 12, 33, 51
MaeIII GTNAC 1 cut(s) 13
MalI GATC 4 cut(s) 312, 515, 732, 765
MboI GATC 4 cut(s) 310, 513, 730, 763
MboII GAAGA 4 cut(s) 310, 404, 704, 767
MhlI GDGCHC 2 cut(s) 235, 471
MlsI TGGCCA 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 7 cut(s) 91, 94, 154, 311, 349, 631, 681
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 161
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 1 cut(s) 494
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 5
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 2 cut(s) 187, 647
NciI CCSGG 1 cut(s) 495
NcoI CCATGG 1 cut(s) 236
NdeII GATC 4 cut(s) 310, 513, 730, 763
NlaIII CATG 3 cut(s) 240, 284, 777
NlaIV GGNNCC 4 cut(s) 79, 404, 475, 781
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 777
PciI ACATGT 1 cut(s) 773
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 3 cut(s) 140, 266, 758
PkrI GCNGC 1 cut(s) 750
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 78
PscI ACATGT 1 cut(s) 773
Psp124BI GAGCTC 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 78
PspN4I GGNNCC 4 cut(s) 79, 404, 475, 781
PspPI GGNCC 1 cut(s) 78
PspPPI RGGWCCY 1 cut(s) 78
PstI CTGCAG 1 cut(s) 753
PstNI CAGNNNCTG 1 cut(s) 748
RsaI GTAC 3 cut(s) 173, 404, 772
RsaNI GTAC 3 cut(s) 172, 403, 771
SacI GAGCTC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 1 cut(s) 749
Sau3AI GATC 4 cut(s) 310, 513, 730, 763
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 2 cut(s) 235, 471
SfcI CTRYAG 3 cut(s) 126, 387, 749
SinI GGWCC 1 cut(s) 78
SstI GAGCTC 1 cut(s) 235
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 1 cut(s) 708
TaiI ACGT 3 cut(s) 15, 36, 54
TaqI TCGA 4 cut(s) 138, 146, 309, 787
TaqII GACCGA 1 cut(s) 284
TatI WGTACW 1 cut(s) 770
TfiI GAWTC 3 cut(s) 140, 266, 758
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 413
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 1 cut(s) 748
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 1 cut(s) 269
TspRI CASTG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 78
XceI RCATGY 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.