Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005790

Physical Location & Seq

Genomic Coordinates Forward (+)

5044283 .. 5059198

Gene Structure 14916 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1042900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGATCAGGCTCATAACAAAATCCAAATCTTTCAGGAAACCAAGCTCCTCATCCTCATTTCTATCTTCAATCTCTCCCAGTCCATCTAACCCATTTTCCTCTTCGCCAAAAACAACCCATATACCAAATGGGGGCTATCTCTACGTTCAGCTTCTGGGTCGAAATTTTGCTTCTTTACGATCCCAGATTGGTTCCCGGCCGGAAGTCTCATCTGGGTATTGCCAAAATTGGGTAAATGTTCGGGCAAATGGGACTTTGGGGTCTCAGACCAGGAGTTTGTGCAGTGAACCGGACAGAGAGTCTATTCAATATGATGTTGTCATTGTTGGGGCTGGACCTGCCGGTCTATCGGCAGCGATAAGGATGAAGCAGCTGTGCCGCGAGAAGGATGTTGATTTGTCAGTGTGCGTTGTGGAGAAAGGAGCTGAAGTTGGTGCTCACATTATTTCTGGTAATGTTTTTGAGCCCCGGGCACTGAATGAACTTCTCCCACAATGGAAAGATGAAGAGTCACCAATCACTGTACCTGTTACTTCTGACAAGTTTTGGCTTCTTACAAAGGATCGTGCACTTTCACTGCCATCTCCTTTTGATAATAGGGGAAACTATGTTATAAGTTTAAGTGAGTTGGTACGATGGATGGGAGTGAAGGCAGAAGAGTTGGGAGTAGAAATATATCCTGGCTTTGCTGCTAGTGAGATATTATATGATACAAACGACACGGTTGTTGGCATTGGAACTAATGATATGGGAATTGCCAAAGATGGTTCAAAGAAGGAAAATTTCCAGCGGCGAGTAACACTTCTAGCTGAAGGGTGTCGAGGATCATTATCAGAGGTTTGGGAAATTGATGAAGGAAAGCATGAGCCCGGTGCTGTGTTGCACACTCTGGGTTGGCCCCTGGATCCAAAGACATACGGCGGTTCATTTTTATACCACATGAAAGATAGACAGATTTCTATTGGCTTTGTGGTTGCTTTGAATTATCATAACCCTTTCTTGAACCCTTACGAAGAATTCCAGAAATTCAAACATCATCCTGCAATCAAAACACTGCTGGAAGGTGGGACTGTTCTTCAATATGGGGCTCGCACCTTGAATGAGGGTGGTTTTCAGTCTATCCCGTATCCAGTTTTCCCTGGAGGAGCACTTATTGGATGTTCAGCTGGCTTTTTAAATGTACCTAAAATAAAAGGAACTCATACTGCAATGAAATCAGGCATGCTAGCTGCAGAAGCTACATTCAGTCTACTTCATGAAGGCTCACGCATGGAAAAATATTGGGATGCTTTAAGGAATTCTTGGATATGGGAAGAACTGTACCGAGCTCGGAACTTCCGACCAGCATTCGAATATGGACTTATTCCTGGCCTGGCCTTAAGTGCTTTAGAACATTACATAATGAAAGGAAGATCTCCCTGGACGCTGAAGCATGGAAAACCAGACCATGAAGCAACAAATGTTGCACAGTTCCACTCTCCAATTCAATATCCAAAGCCAGATGGGGTTTTATCTTTCGATATTCCAACCTCCTTACACAGGAGCAACACAAATCATGAACATGACCAACCACCTCACCTTCGGTTGAGAGACCCCAACATTCCTGAACTTGTAAATTTGCCAGAGTATGCTGGCCCCGAGTCACGATACTGTCCTGCACGTGTATACGAGTATGTCCCTGATGAAGAGGGTCAGCCGAAGTTGCAGATCAATGCTCAGAATTGCCTACATTGCAAGGCATGCGATATCAAAGACCCGAAGCAAAACATCAACTGGACTCCACCGGAAGGAGGTGGCGGTCCAGGCTACTCCATAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004174 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005759 GO:0006082 GO:0006091 GO:0006520 GO:0006551 GO:0006552 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009055 GO:0009056 GO:0009062 GO:0009063 GO:0009081 GO:0009083 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009646 GO:0009987 GO:0015980 GO:0016020 GO:0016021 GO:0016042 GO:0016054 GO:0016491 GO:0016645 GO:0016649 GO:0016722 GO:0017133 GO:0019395 GO:0019752 GO:0019866 GO:0022900 GO:0022904 GO:0030258 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0032592 GO:0032787 GO:0032991 GO:0033539 GO:0034440 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043783 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045251 GO:0045333 GO:0046395 GO:0048037 GO:0048038 GO:0048039 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0055114 GO:0070013 GO:0071704 GO:0072329 GO:0097159 GO:0098573 GO:0098798 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ETF_QO FAD_binding_2 NAD_binding_8

Protein Analysis

607

Amino Acids

67.21

Weight (kDa)

6.69

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETFQO_UQ-bd	PF21162	277 - 366	7.2e-39	ETF-QO, ubiquinone-binding
Fer4_ETF_QO	PF05187	502 - 604	1.1e-45	Electron transfer flavoprotein-ubiquinone oxidoreductase, 4Fe-4S

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005790)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43400 AT2G43400
fragaria_vesca	FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960
malus_domestica	MD04G1042900.v1.1
prunus_persica	Prupe.1G197500_v2.0.a1
pyrus_communis	pycom04g03770
rosa_chinensis	RchiOBHm_Chr4g0406421
rosa_laevigata	RLG00000008773
rosa_multiflora	Rmu_ssc0000186.1_g000005
rosa_roxburghii	Rroxscaffold_1G00028480 Rroxscaffold_5G00351230
rosa_rugosa	Rorug04G0069200
rosa_samantha	Rh4AG138000 Rh4BG135300 Rh4CG145700 Rh4DG132700
rosa_wichuraiana	Rw4G011250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	614
AasI	GACNNNNNNGTC	2 cut(s)	259, 342
Acc36I	ACCTGC	1 cut(s)	346
AccI	GTMKAC	2 cut(s)	1249, 1665
AccII	CGCG	1 cut(s)	381
AciI	CCGC	4 cut(s)	379, 790, 921, 1797
AclWI	GGATC	5 cut(s)	174, 570, 832, 899, 912
AcoI	YGGCCR	1 cut(s)	197
AcsI	RAATTY	6 cut(s)	163, 781, 1016, 1025, 1297, 1615
AcuI	CTGAAG	3 cut(s)	447, 831, 1448
AcvI	CACGTG	1 cut(s)	1661
AfaI	GTAC	4 cut(s)	525, 633, 1182, 1322
AfiI	CCNNNNNNNGG	6 cut(s)	131, 229, 385, 1539, 1787, 1790
AflII	CTTAAG	1 cut(s)	1378
AflIII	ACRYGT	1 cut(s)	1660
AgsI	TTSAA	9 cut(s)	69, 308, 771, 982, 1003, 1030, 1079, 1099, 1487
AhdI	GACNNNNNGTC	1 cut(s)	298
AjnI	CCWGG	8 cut(s)	269, 679, 900, 1138, 1366, 1371, 1418, 1801
AluBI	AGCT	9 cut(s)	45, 151, 373, 425, 809, 1166, 1229, 1238, 1328
AluI	AGCT	9 cut(s)	45, 151, 373, 425, 809, 1166, 1229, 1238, 1328
Alw21I	GWGCWC	4 cut(s)	439, 571, 1150, 1330
Alw26I	GTCTC	3 cut(s)	211, 267, 1584
Alw44I	GTGCAC	1 cut(s)	567
AlwI	GGATC	5 cut(s)	174, 570, 832, 899, 912
AlwNI	CAGNNNCTG	1 cut(s)	154
Ama87I	CYCGRG	2 cut(s)	468, 1637
AoxI	GGCC	5 cut(s)	197, 896, 1369, 1374, 1633
ApaLI	GTGCAC	1 cut(s)	567
ApeKI	GCWGC	4 cut(s)	353, 370, 689, 1229
ApoI	RAATTY	6 cut(s)	163, 781, 1016, 1025, 1297, 1615
ArsI	GACNNNNNNTTYG	2 cut(s)	259, 291
AspS9I	GGNCC	4 cut(s)	335, 897, 1634, 1799
AsuC2I	CCSGG	4 cut(s)	196, 469, 470, 870
AsuHPI	GGTGA	2 cut(s)	504, 1568
AsuII	TTCGAA	1 cut(s)	1350
AsuNHI	GCTAGC	1 cut(s)	1225
AvaI	CYCGRG	2 cut(s)	468, 1637
AvaII	GGWCC	2 cut(s)	335, 1799
BaeGI	GKGCMC	2 cut(s)	475, 571
BamHI	GGATCC	1 cut(s)	904
BanII	GRGCYC	4 cut(s)	468, 870, 1090, 1330
BbrPI	CACGTG	1 cut(s)	1661
Bbv12I	GWGCWC	4 cut(s)	439, 571, 1150, 1330
BbvI	GCAGC	4 cut(s)	365, 382, 676, 1216
BccI	CCATC	6 cut(s)	91, 589, 630, 634, 758, 1496
BceAI	ACGGC	1 cut(s)	934
BciT130I	CCWGG	8 cut(s)	271, 681, 902, 1140, 1368, 1373, 1420, 1803
BciVI	GTATCC	1 cut(s)	1137
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	4 cut(s)	196, 469, 470, 870
BcoDI	GTCTC	3 cut(s)	211, 267, 1584
BfaI	CTAG	3 cut(s)	693, 806, 1226
BfmI	CTRYAG	1 cut(s)	1230
BfrI	CTTAAG	1 cut(s)	1378
BfuAI	ACCTGC	1 cut(s)	346
BfuI	GTATCC	1 cut(s)	1137
BglII	AGATCT	1 cut(s)	1412
BisI	GCNGC	6 cut(s)	354, 371, 379, 690, 791, 1230
BlsI	GCNGC	6 cut(s)	355, 372, 380, 691, 792, 1231
Bme18I	GGWCC	2 cut(s)	335, 1799
BmeRI	GACNNNNNGTC	1 cut(s)	298
BmeT110I	CYCGRG	2 cut(s)	468, 1637
BmgT120I	GGNCC	4 cut(s)	335, 897, 1634, 1799
BmiI	GGNNCC	4 cut(s)	193, 899, 906, 1636
BmrI	ACTGGG	1 cut(s)	72
BmsI	GCATC	1 cut(s)	1276
BmtI	GCTAGC	1 cut(s)	1229
BmuI	ACTGGG	1 cut(s)	72
BpmI	CTGGAG	1 cut(s)	1161
Bpu14I	TTCGAA	1 cut(s)	1350
BpuMI	CCSGG	4 cut(s)	196, 469, 470, 870
BsaAI	YACGTR	1 cut(s)	1661
BsaBI	GATNNNNATC	1 cut(s)	829
BsaI	GGTCTC	2 cut(s)	267, 1584
BsaJI	CCNNGG	5 cut(s)	467, 468, 900, 1138, 1418
BsaWI	WCCGGW	2 cut(s)	289, 1783
BsaXI	ACNNNNNCTCC	2 cut(s)	414, 444
Bsc4I	CCNNNNNNNGG	6 cut(s)	131, 229, 385, 1539, 1787, 1790
Bse118I	RCCGGY	1 cut(s)	341
Bse1I	ACTGG	3 cut(s)	78, 1130, 1778
Bse3DI	GCAATG	2 cut(s)	1215, 1729
Bse8I	GATNNNNATC	1 cut(s)	829
BseBI	CCWGG	8 cut(s)	271, 681, 902, 1140, 1368, 1373, 1420, 1803
BseDI	CCNNGG	5 cut(s)	467, 468, 900, 1138, 1418
BseGI	GGATG	7 cut(s)	50, 369, 394, 645, 1036, 1163, 1291
BseJI	GATNNNNATC	1 cut(s)	829
BseLI	CCNNNNNNNGG	6 cut(s)	131, 229, 385, 1539, 1787, 1790
BseMI	GCAATG	2 cut(s)	1215, 1729
BseMII	CTCAG	2 cut(s)	278, 1730
BseNI	ACTGG	3 cut(s)	78, 1130, 1778
BseRI	GAGGAG	2 cut(s)	37, 1158
BseSI	GKGCMC	2 cut(s)	475, 571
BseX3I	CGGCCG	1 cut(s)	197
BseXI	GCAGC	4 cut(s)	365, 382, 676, 1216
BsgI	GTGCAG	2 cut(s)	301, 1641
Bsh1236I	CGCG	1 cut(s)	381
Bsh1285I	CGRYCG	1 cut(s)	200
BshFI	GGCC	5 cut(s)	199, 898, 1371, 1376, 1635
BsiEI	CGRYCG	1 cut(s)	200
BsiHKAI	GWGCWC	4 cut(s)	439, 571, 1150, 1330
BsiHKCI	CYCGRG	2 cut(s)	468, 1637
BsiSI	CCGG	7 cut(s)	196, 200, 290, 342, 469, 870, 1784
BslFI	GGGAC	3 cut(s)	265, 1081, 1661
BslI	CCNNNNNNNGG	6 cut(s)	131, 229, 385, 1539, 1787, 1790
BsmAI	GTCTC	3 cut(s)	211, 267, 1584
BsmFI	GGGAC	3 cut(s)	265, 1081, 1661
BsmI	GAATGC	1 cut(s)	1346
BsnI	GGCC	5 cut(s)	199, 898, 1371, 1376, 1635
Bso31I	GGTCTC	2 cut(s)	267, 1584
BsoBI	CYCGRG	2 cut(s)	468, 1637
Bsp119I	TTCGAA	1 cut(s)	1350
Bsp1286I	GDGCHC	8 cut(s)	439, 468, 475, 571, 870, 1090, 1150, 1330
Bsp143I	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
BspACI	CCGC	4 cut(s)	379, 790, 921, 1797
BspANI	GGCC	5 cut(s)	199, 898, 1371, 1376, 1635
BspCNI	CTCAG	2 cut(s)	277, 1729
BspFNI	CGCG	1 cut(s)	381
BspHI	TCATGA	2 cut(s)	1255, 1555
BspLI	GGNNCC	4 cut(s)	193, 899, 906, 1636
BspMAI	CTGCAG	1 cut(s)	1234
BspMI	ACCTGC	1 cut(s)	346
BspOI	GCTAGC	1 cut(s)	1229
BspPI	GGATC	5 cut(s)	174, 570, 832, 899, 912
BspT104I	TTCGAA	1 cut(s)	1350
BspTI	CTTAAG	1 cut(s)	1378
BspTNI	GGTCTC	2 cut(s)	267, 1584
BsrDI	GCAATG	2 cut(s)	1215, 1729
BsrFI	RCCGGY	1 cut(s)	341
BsrI	ACTGG	3 cut(s)	78, 1130, 1778
BssAI	RCCGGY	1 cut(s)	341
BssECI	CCNNGG	5 cut(s)	467, 468, 900, 1138, 1418
BssMI	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
BssNAI	GTATAC	1 cut(s)	1666
Bst1107I	GTATAC	1 cut(s)	1666
Bst2UI	CCWGG	8 cut(s)	271, 681, 902, 1140, 1368, 1373, 1420, 1803
Bst4CI	ACNGT	6 cut(s)	523, 724, 1072, 1320, 1470, 1652
Bst6I	CTCTTC	4 cut(s)	106, 501, 651, 1680
BstAFI	CTTAAG	1 cut(s)	1378
BstAPI	GCANNNNNTGC	1 cut(s)	1740
BstBAI	YACGTR	1 cut(s)	1661
BstBI	TTCGAA	1 cut(s)	1350
BstC8I	GCNNGC	6 cut(s)	1090, 1168, 1223, 1227, 1633, 1741
BstDEI	CTNAG	2 cut(s)	264, 1716
BstENI	CCTNNNNNAGG	1 cut(s)	1537
BstF5I	GGATG	7 cut(s)	50, 369, 394, 645, 1036, 1163, 1291
BstFNI	CGCG	1 cut(s)	381
BstKTI	GATC	7 cut(s)	6, 182, 565, 827, 907, 1415, 1710
BstMAI	GTCTC	3 cut(s)	211, 267, 1584
BstMBI	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
BstMCI	CGRYCG	1 cut(s)	200
BstMWI	GCNNNNNNNGC	7 cut(s)	338, 1235, 1382, 1702, 1731, 1740, 1803
BstNI	CCWGG	8 cut(s)	271, 681, 902, 1140, 1368, 1373, 1420, 1803
BstNSI	RCATGY	2 cut(s)	1225, 1743
BstSFI	CTRYAG	1 cut(s)	1230
BstSLI	GKGCMC	2 cut(s)	475, 571
BstUI	CGCG	1 cut(s)	381
BstV1I	GCAGC	4 cut(s)	365, 382, 676, 1216
BstX2I	RGATCY	2 cut(s)	904, 1412
BstYI	RGATCY	2 cut(s)	904, 1412
BstZ17I	GTATAC	1 cut(s)	1666
BstZI	CGGCCG	1 cut(s)	197
BsuI	GTATCC	1 cut(s)	1137
BsuRI	GGCC	5 cut(s)	199, 898, 1371, 1376, 1635
BtsCI	GGATG	7 cut(s)	50, 369, 394, 645, 1036, 1163, 1291
BtsI	GCAGTG	3 cut(s)	289, 575, 1052
BtsIMutI	CAGTG	6 cut(s)	289, 408, 473, 519, 575, 1052
BveI	ACCTGC	1 cut(s)	346
Cac8I	GCNNGC	6 cut(s)	1090, 1168, 1223, 1227, 1633, 1741
CaiI	CAGNNNCTG	1 cut(s)	154
CciI	TCATGA	2 cut(s)	1255, 1555
Cfr10I	RCCGGY	1 cut(s)	341
Cfr13I	GGNCC	4 cut(s)	335, 897, 1634, 1799
Cfr9I	CCCGGG	1 cut(s)	468
CseI	GACGC	1 cut(s)	1432
Csp6I	GTAC	4 cut(s)	524, 632, 1181, 1321
CviQI	GTAC	4 cut(s)	524, 632, 1181, 1321
DdeI	CTNAG	2 cut(s)	264, 1716
DpnI	GATC	7 cut(s)	5, 181, 564, 826, 906, 1414, 1709
DpnII	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
DraI	TTTAAA	1 cut(s)	1176
DrdI	GACNNNNNNGTC	2 cut(s)	259, 342
DriI	GACNNNNNGTC	1 cut(s)	298
DseDI	GACNNNNNNGTC	2 cut(s)	259, 342
EaeI	YGGCCR	1 cut(s)	197
EagI	CGGCCG	1 cut(s)	197
Eam1104I	CTCTTC	4 cut(s)	106, 501, 651, 1680
Eam1105I	GACNNNNNGTC	1 cut(s)	298
EarI	CTCTTC	4 cut(s)	106, 501, 651, 1680
Ecl136II	GAGCTC	1 cut(s)	1328
EclXI	CGGCCG	1 cut(s)	197
Eco24I	GRGCYC	4 cut(s)	468, 870, 1090, 1330
Eco31I	GGTCTC	2 cut(s)	267, 1584
Eco32I	GATATC	1 cut(s)	1747
Eco47I	GGWCC	2 cut(s)	335, 1799
Eco52I	CGGCCG	1 cut(s)	197
Eco53kI	GAGCTC	1 cut(s)	1328
Eco57I	CTGAAG	3 cut(s)	447, 831, 1448
Eco72I	CACGTG	1 cut(s)	1661
Eco88I	CYCGRG	2 cut(s)	468, 1637
EcoICRI	GAGCTC	1 cut(s)	1328
EcoNI	CCTNNNNNAGG	1 cut(s)	1537
EcoRI	GAATTC	2 cut(s)	1016, 1297
EcoRII	CCWGG	8 cut(s)	269, 679, 900, 1138, 1366, 1371, 1418, 1801
EcoRV	GATATC	1 cut(s)	1747
EcoT38I	GRGCYC	4 cut(s)	468, 870, 1090, 1330
FalI	AAGNNNNNCTT	2 cut(s)	1285, 1317
FaqI	GGGAC	3 cut(s)	265, 1081, 1661
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	2 cut(s)	1249, 1665
Fnu4HI	GCNGC	6 cut(s)	354, 371, 379, 690, 791, 1230
FokI	GGATG	7 cut(s)	37, 376, 401, 652, 1023, 1170, 1298
FriOI	GRGCYC	4 cut(s)	468, 870, 1090, 1330
Fsp4HI	GCNGC	6 cut(s)	354, 371, 379, 690, 791, 1230
FspBI	CTAG	3 cut(s)	693, 806, 1226
GluI	GCNGC	6 cut(s)	354, 371, 379, 690, 791, 1230
GsuI	CTGGAG	1 cut(s)	1161
HaeIII	GGCC	5 cut(s)	199, 898, 1371, 1376, 1635
HapII	CCGG	7 cut(s)	196, 200, 290, 342, 469, 870, 1784
HgaI	GACGC	1 cut(s)	1432
HinfI	GANTC	4 cut(s)	299, 509, 1640, 1777
HpaII	CCGG	7 cut(s)	196, 200, 290, 342, 469, 870, 1784
HphI	GGTGA	2 cut(s)	504, 1568
Hpy166II	GTNNAC	4 cut(s)	287, 569, 1250, 1666
Hpy188I	TCNGA	6 cut(s)	267, 538, 835, 1332, 1340, 1719
Hpy188III	TCNNGA	7 cut(s)	34, 1000, 1021, 1256, 1556, 1604, 1644
Hpy8I	GTNNAC	4 cut(s)	287, 569, 1250, 1666
HpyAV	CCTTC	9 cut(s)	379, 643, 769, 806, 848, 1055, 1253, 1589, 1781
HpyCH4III	ACNGT	6 cut(s)	523, 724, 1072, 1320, 1470, 1652
HpyCH4IV	ACGT	2 cut(s)	144, 1660
HpyF10VI	GCNNNNNNNGC	7 cut(s)	338, 1235, 1382, 1702, 1731, 1740, 1803
HpyF3I	CTNAG	2 cut(s)	264, 1716
HpySE526I	ACGT	2 cut(s)	144, 1660
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
LmnI	GCTCC	4 cut(s)	50, 422, 1145, 1542
Lsp1109I	GCAGC	4 cut(s)	365, 382, 676, 1216
LweI	GCATC	1 cut(s)	1276
MaeI	CTAG	3 cut(s)	693, 806, 1226
MaeII	ACGT	2 cut(s)	144, 1660
MaeIII	GTNAC	4 cut(s)	510, 529, 796, 1641
MalI	GATC	7 cut(s)	5, 181, 564, 826, 906, 1414, 1709
MboI	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
MboII	GAAGA	9 cut(s)	57, 93, 518, 668, 1025, 1067, 1325, 1422, 1697
MflI	RGATCY	2 cut(s)	904, 1412
MhlI	GDGCHC	8 cut(s)	439, 468, 475, 571, 870, 1090, 1150, 1330
MlyI	GAGTC	4 cut(s)	308, 518, 1649, 1771
MmeI	TCCRAC	2 cut(s)	1363, 1550
MseI	TTAA	4 cut(s)	620, 1175, 1292, 1379
MslI	CAYNNNNRTG	1 cut(s)	1560
MspA1I	CMGCKG	3 cut(s)	373, 790, 1166
MspCI	CTTAAG	1 cut(s)	1378
MspI	CCGG	7 cut(s)	196, 200, 290, 342, 469, 870, 1784
Mva1269I	GAATGC	1 cut(s)	1346
MvaI	CCWGG	8 cut(s)	271, 681, 902, 1140, 1368, 1373, 1420, 1803
MvnI	CGCG	1 cut(s)	381
MwoI	GCNNNNNNNGC	7 cut(s)	338, 1235, 1382, 1702, 1731, 1740, 1803
NciI	CCSGG	4 cut(s)	196, 469, 470, 870
NdeII	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
NheI	GCTAGC	1 cut(s)	1225
NlaIV	GGNNCC	4 cut(s)	193, 899, 906, 1636
NmuCI	GTSAC	2 cut(s)	510, 1641
NspI	RCATGY	2 cut(s)	1225, 1743
NspV	TTCGAA	1 cut(s)	1350
PaeI	GCATGC	2 cut(s)	1225, 1743
PagI	TCATGA	2 cut(s)	1255, 1555
PcsI	WCGNNNNNNNCGW	1 cut(s)	1336
PctI	GAATGC	1 cut(s)	1346
PkrI	GCNGC	6 cut(s)	355, 372, 380, 691, 792, 1231
PleI	GAGTC	4 cut(s)	307, 517, 1648, 1771
PmaCI	CACGTG	1 cut(s)	1661
PmlI	CACGTG	1 cut(s)	1661
PpsI	GAGTC	4 cut(s)	307, 517, 1648, 1771
Ppu21I	YACGTR	1 cut(s)	1661
PsiI	TTATAA	1 cut(s)	614
Psp124BI	GAGCTC	1 cut(s)	1330
Psp6I	CCWGG	8 cut(s)	269, 679, 900, 1138, 1366, 1371, 1418, 1801
PspCI	CACGTG	1 cut(s)	1661
PspGI	CCWGG	8 cut(s)	269, 679, 900, 1138, 1366, 1371, 1418, 1801
PspN4I	GGNNCC	4 cut(s)	193, 899, 906, 1636
PspPI	GGNCC	4 cut(s)	335, 897, 1634, 1799
PstI	CTGCAG	1 cut(s)	1234
PstNI	CAGNNNCTG	1 cut(s)	154
PsuI	RGATCY	2 cut(s)	904, 1412
PvuII	CAGCTG	2 cut(s)	373, 1166
RsaI	GTAC	4 cut(s)	525, 633, 1182, 1322
RsaNI	GTAC	4 cut(s)	524, 632, 1181, 1321
RseI	CAYNNNNRTG	1 cut(s)	1560
SacI	GAGCTC	1 cut(s)	1330
SaqAI	TTAA	4 cut(s)	620, 1175, 1292, 1379
SatI	GCNGC	6 cut(s)	354, 371, 379, 690, 791, 1230
Sau3AI	GATC	7 cut(s)	3, 179, 562, 824, 904, 1412, 1707
Sau96I	GGNCC	4 cut(s)	335, 897, 1634, 1799
SchI	GAGTC	4 cut(s)	308, 518, 1649, 1771
SduI	GDGCHC	8 cut(s)	439, 468, 475, 571, 870, 1090, 1150, 1330
SfaNI	GCATC	1 cut(s)	1276
SfcI	CTRYAG	1 cut(s)	1230
SfuI	TTCGAA	1 cut(s)	1350
SinI	GGWCC	2 cut(s)	335, 1799
SmaI	CCCGGG	1 cut(s)	470
SmiMI	CAYNNNNRTG	1 cut(s)	1560
SmlI	CTYRAG	1 cut(s)	1378
SmoI	CTYRAG	1 cut(s)	1378
SphI	GCATGC	2 cut(s)	1225, 1743
SsiI	CCGC	4 cut(s)	379, 790, 921, 1797
SspI	AATATT	1 cut(s)	1280
SspMI	CTAG	3 cut(s)	693, 806, 1226
SstI	GAGCTC	1 cut(s)	1330
TaaI	ACNGT	6 cut(s)	523, 724, 1072, 1320, 1470, 1652
TaiI	ACGT	2 cut(s)	147, 1663
TaqI	TCGA	4 cut(s)	160, 820, 1350, 1518
TauI	GCSGC	2 cut(s)	381, 793
Tru1I	TTAA	4 cut(s)	620, 1175, 1292, 1379
Tru9I	TTAA	4 cut(s)	620, 1175, 1292, 1379
TscAI	CASTG	6 cut(s)	289, 408, 480, 526, 582, 1059
TseFI	GTSAC	2 cut(s)	510, 1641
TseI	GCWGC	4 cut(s)	353, 370, 689, 1229
Tsp45I	GTSAC	2 cut(s)	510, 1641
TspMI	CCCGGG	1 cut(s)	468
TspRI	CASTG	6 cut(s)	289, 408, 480, 526, 582, 1059
Vha464I	CTTAAG	1 cut(s)	1378
VneI	GTGCAC	1 cut(s)	567
VpaK11BI	GGWCC	2 cut(s)	335, 1799
XagI	CCTNNNNNAGG	1 cut(s)	1537
XapI	RAATTY	6 cut(s)	163, 781, 1016, 1025, 1297, 1615
XceI	RCATGY	2 cut(s)	1225, 1743
XcmI	CCANNNNNNNNNTGG	1 cut(s)	125
XmaI	CCCGGG	1 cut(s)	468
XmiI	GTMKAC	2 cut(s)	1249, 1665
XspI	CTAG	3 cut(s)	693, 806, 1226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.