Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0005790

Physical Location & Seq

Genomic Coordinates Reverse (-)

23945674 .. 23959155

Gene Structure 13482 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG135300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGTCGATCATGCTGAGGCATATCCTGAAACGAAATTCGAATTCGTACATTGGGCGGCTGGCTTCCGCTTCCGCGTCTGGACGGCTATTCTCGTGGAGGAGGAGGAGGACTTTCTGCAGTGAACCGCAGAGAGAGTCTATTCAATACGACGTCGTGATCGTTGGGGCGGGACCGGCTGGTTTATCGGCGGCGATTAGGTTCAAACAATTGTGCCGTGAAAATGATACTGATTTATCAGTCTGCGTTGTGGAGAAAGGAGCTGAAGTCGGTGCTCACATTATTTCTGGTAATGTCTTTGAACCCCGGGCTCTGGATGAACTTCTCCCGCAATGGAGACAGGAAGAGTCACCCATCACCGTCCCTGTTTCTTCTGACAAGTTTTTGTTACTTACAAAGAATCATGCATTTTCGCTGCCTTGTCCTTTTAATAACAGAGGAAACTATGTCATAAGTTTAAGTCAGTTAGTACGATGGATGGGAGTGAAGGCAGAAGAATTGGGAGTAGAAATATATCCAGGCTTTGCTGCTAGTGAGATATTATATGATTCAAATGACACTGTTGTTGGCATTCGAACTAATGATATGGGAATATCCAAAGATGGTTCAAAGAAGGAAAATTTTCAACCTGGCATAGAATTGAAAGGGCGGGTCACACTTCTAGCTGAAGGGTGTCGAGGATCATTGTCAGAGAAAATAATGGATAAATACAACTTGAGAGAGAAGGGGCATGCTCAGCATCAGACTTATGCTTTGGGAATCAAAGAGGTTTGGGAAATTGATGAGAGAAAACACAAACCAGGTGCTGTGATTCACACGCTGGGTTGGCCCTTGGATCAGAAGACATATGGAGGTTCATTTCTGTACCACATGAAAGATAGACAGATTTCTATTGGCTTTGTGGTTGCCTTGAATTATCAAAACCCTTTCTTAAGTCCATATGAAGAATTCCAGAAATTTAAGCATCATCCTGCAATCAAGCCACTGCTAGAGGGTGGGACTGTTCTTCAATATGGCGCTCGCACCTTGAATGAAGGTGGCTTTCAGTCTATCCCATATCCAGCTTTCCCTGGAGGAGCAGTTATTGGATGCTCTGCTGGCTTTTTAAATGTACCAAAAATTAAGGGAACACATACTGCAATGAAATCAGGCATGCTAGCTGCGGAAGCATCGTTTGGTGTTCTTCATAAAGGCTCAAGTATGGAAAAATACTGGGATGCTTTAAGGAATTCGTGGATATGGGAAGAATTATATAGAGCACGGAACTTCCGACCGGCATTTGAGTATGGACTTATTCCTGGCTTGGCCATAAGTGCGTTAGAACACTACATAATGAAAGGGAGAGCTGCCTTAACGCTGAAGCATGGAAAACCTGATAATGAAGCAACAAATGTTGCAGAGTTCCACTGTCCAATTAAATATCCAAAGCCAGATGGGGTTTTATCTTTTGATATTCCAACCTCCCTACACAGGAGCAATACAAATCATGAGCATGACCAACCAGCTCACCTTCGGTTGAAGGACCCCAAGAAGCCTGAACTTGTCAACTTGCCAGTGTATGCTGGTCCTGAGTCACGATATTGTCCTGCACGTGTTTACGAGTATAACCCTGATGAAACGGGTCAGCTGAAGTTGCAGATCAACGCTCAGAATTGCTTACATTGCAAGGCTTGCGATGTCAAAGACCCTAAGCAAAACATAGAGTGGACTGCGCCAGAAGGAGGTGGTGGTCCAGGCTATTCAATAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004174 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005759 GO:0006082 GO:0006091 GO:0006520 GO:0006551 GO:0006552 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009055 GO:0009056 GO:0009062 GO:0009063 GO:0009081 GO:0009083 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009646 GO:0009987 GO:0015980 GO:0016020 GO:0016021 GO:0016042 GO:0016054 GO:0016491 GO:0016645 GO:0016649 GO:0016722 GO:0017133 GO:0019395 GO:0019752 GO:0019866 GO:0022900 GO:0022904 GO:0030258 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0032592 GO:0032787 GO:0032991 GO:0033539 GO:0034440 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043783 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045251 GO:0045333 GO:0046395 GO:0048037 GO:0048038 GO:0048039 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0055114 GO:0070013 GO:0071704 GO:0072329 GO:0097159 GO:0098573 GO:0098798 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ETF_QO FAD_binding_2 NAD_binding_8

Protein Analysis

582

Amino Acids

64.93

Weight (kDa)

8.65

Isoelectric Point (pI)

38.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETFQO_UQ-bd	PF21162	249 - 342	1e-43	ETF-QO, ubiquinone-binding
Fer4_ETF_QO	PF05187	478 - 580	9.6e-45	Electron transfer flavoprotein-ubiquinone oxidoreductase, 4Fe-4S

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005790)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43400 AT2G43400
fragaria_vesca	FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960 FvH4_4g10960
malus_domestica	MD04G1042900.v1.1
prunus_persica	Prupe.1G197500_v2.0.a1
pyrus_communis	pycom04g03770
rosa_chinensis	RchiOBHm_Chr4g0406421
rosa_laevigata	RLG00000008773
rosa_multiflora	Rmu_ssc0000186.1_g000005
rosa_roxburghii	Rroxscaffold_1G00028480 Rroxscaffold_5G00351230
rosa_rugosa	Rorug04G0069200
rosa_samantha	Rh4AG138000 Rh4BG135300 Rh4CG145700 Rh4DG132700
rosa_wichuraiana	Rw4G011250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	153
AccII	CGCG	1 cut(s)	74
AciI	CCGC	9 cut(s)	55, 66, 72, 125, 167, 188, 326, 646, 1160
AclWI	GGATC	2 cut(s)	685, 840
AcoI	YGGCCR	1 cut(s)	1302
AcsI	RAATTY	6 cut(s)	34, 40, 616, 944, 953, 1225
AcuI	CTGAAG	4 cut(s)	282, 684, 1376, 1646
AcvI	CACGTG	1 cut(s)	1589
AcyI	GRCGYC	1 cut(s)	150
AfaI	GTAC	4 cut(s)	47, 468, 863, 1110
AfiI	CCNNNNNNNGG	3 cut(s)	310, 1467, 1718
AflII	CTTAAG	1 cut(s)	928
AflIII	ACRYGT	1 cut(s)	1588
AjnI	CCWGG	6 cut(s)	514, 625, 796, 1066, 1294, 1729
AjuI	GAANNNNNNNTTGG	2 cut(s)	1155, 1187
AluBI	AGCT	7 cut(s)	260, 662, 1061, 1157, 1343, 1502, 1624
AluI	AGCT	7 cut(s)	260, 662, 1061, 1157, 1343, 1502, 1624
Alw21I	GWGCWC	2 cut(s)	274, 1258
Alw26I	GTCTC	1 cut(s)	328
AlwI	GGATC	2 cut(s)	685, 840
AlwNI	CAGNNNCTG	1 cut(s)	803
Ama87I	CYCGRG	1 cut(s)	303
AoxI	GGCC	2 cut(s)	824, 1302
ApeKI	GCWGC	4 cut(s)	412, 524, 1157, 1343
ApoI	RAATTY	6 cut(s)	34, 40, 616, 944, 953, 1225
ArsI	GACNNNNNNTTYG	2 cut(s)	733, 765
Asp700I	GAANNNNTTC	1 cut(s)	618
AspLEI	GCGC	2 cut(s)	1016, 1711
AspS9I	GGNCC	5 cut(s)	170, 825, 1519, 1562, 1727
AsuC2I	CCSGG	2 cut(s)	304, 305
AsuHPI	GGTGA	3 cut(s)	339, 346, 1496
AsuII	TTCGAA	2 cut(s)	38, 571
AsuNHI	GCTAGC	1 cut(s)	1153
AvaI	CYCGRG	1 cut(s)	303
AvaII	GGWCC	4 cut(s)	170, 1519, 1562, 1727
BalI	TGGCCA	1 cut(s)	1304
BanII	GRGCYC	1 cut(s)	310
BauI	CACGAG	1 cut(s)	91
BbrPI	CACGTG	1 cut(s)	1589
BbsI	GAAGAC	1 cut(s)	845
Bbv12I	GWGCWC	2 cut(s)	274, 1258
BbvCI	CCTCAGC	1 cut(s)	14
BbvI	GCAGC	4 cut(s)	399, 511, 1144, 1330
BccI	CCATC	5 cut(s)	359, 465, 469, 593, 1424
BceAI	ACGGC	2 cut(s)	98, 198
BciT130I	CCWGG	6 cut(s)	516, 627, 798, 1068, 1296, 1731
BcnI	CCSGG	2 cut(s)	304, 305
BcoDI	GTCTC	1 cut(s)	328
BfaI	CTAG	4 cut(s)	528, 659, 986, 1154
BfmI	CTRYAG	1 cut(s)	115
BfoI	RGCGCY	1 cut(s)	1017
BfrI	CTTAAG	1 cut(s)	928
BisI	GCNGC	6 cut(s)	56, 189, 413, 525, 1158, 1344
BlpI	GCTNAGC	1 cut(s)	732
BlsI	GCNGC	6 cut(s)	57, 190, 414, 526, 1159, 1345
Bme1390I	CCNGG	8 cut(s)	304, 305, 516, 627, 798, 1068, 1296, 1731
Bme18I	GGWCC	4 cut(s)	170, 1519, 1562, 1727
BmeT110I	CYCGRG	1 cut(s)	303
BmgT120I	GGNCC	5 cut(s)	170, 825, 1519, 1562, 1727
BmiI	GGNNCC	2 cut(s)	171, 1521
BmrFI	CCNGG	8 cut(s)	304, 305, 516, 627, 798, 1068, 1296, 1731
BmrI	ACTGGG	1 cut(s)	1219
BmsI	GCATC	5 cut(s)	745, 970, 1076, 1175, 1204
BmtI	GCTAGC	1 cut(s)	1157
BmuI	ACTGGG	1 cut(s)	1219
BpiI	GAAGAC	1 cut(s)	845
BpmI	CTGGAG	1 cut(s)	1089
Bpu10I	CCTNAGC	2 cut(s)	14, 1686
Bpu1102I	GCTNAGC	1 cut(s)	732
Bpu14I	TTCGAA	2 cut(s)	38, 571
BpuEI	CTTGAG	2 cut(s)	733, 1177
BpuMI	CCSGG	2 cut(s)	304, 305
BsaAI	YACGTR	1 cut(s)	1589
BsaHI	GRCGYC	1 cut(s)	150
BsaJI	CCNNGG	4 cut(s)	302, 303, 828, 1066
BsaXI	ACNNNNNCTCC	4 cut(s)	249, 279, 1062, 1092
Bsc4I	CCNNNNNNNGG	3 cut(s)	310, 1467, 1718
Bse118I	RCCGGY	2 cut(s)	172, 1270
Bse1I	ACTGG	2 cut(s)	1214, 1550
Bse3DI	GCAATG	3 cut(s)	335, 1143, 1657
BseBI	CCWGG	6 cut(s)	516, 627, 798, 1068, 1296, 1731
BseDI	CCNNGG	4 cut(s)	302, 303, 828, 1066
BseGI	GGATG	5 cut(s)	319, 480, 964, 1091, 1219
BseLI	CCNNNNNNNGG	3 cut(s)	310, 1467, 1718
BseMI	GCAATG	3 cut(s)	335, 1143, 1657
BseMII	CTCAG	4 cut(s)	5, 746, 1557, 1658
BseNI	ACTGG	2 cut(s)	1214, 1550
BseRI	GAGGAG	4 cut(s)	112, 115, 118, 1086
BseXI	GCAGC	4 cut(s)	399, 511, 1144, 1330
BseYI	CCCAGC	1 cut(s)	817
BsgI	GTGCAG	1 cut(s)	1569
Bsh1236I	CGCG	1 cut(s)	74
Bsh1285I	CGRYCG	1 cut(s)	1271
BshFI	GGCC	2 cut(s)	826, 1304
BsiEI	CGRYCG	1 cut(s)	1271
BsiHKAI	GWGCWC	2 cut(s)	274, 1258
BsiHKCI	CYCGRG	1 cut(s)	303
BsiSI	CCGG	3 cut(s)	173, 304, 1271
BslFI	GGGAC	3 cut(s)	183, 344, 1009
BslI	CCNNNNNNNGG	3 cut(s)	310, 1467, 1718
BsmAI	GTCTC	1 cut(s)	328
BsmFI	GGGAC	3 cut(s)	183, 344, 1009
BsmI	GAATGC	1 cut(s)	567
BsnI	GGCC	2 cut(s)	826, 1304
BsoBI	CYCGRG	1 cut(s)	303
Bsp119I	TTCGAA	2 cut(s)	38, 571
Bsp1286I	GDGCHC	3 cut(s)	274, 310, 1258
Bsp143I	GATC	5 cut(s)	6, 156, 677, 832, 1635
Bsp1720I	GCTNAGC	1 cut(s)	732
BspACI	CCGC	9 cut(s)	55, 66, 72, 125, 167, 188, 326, 646, 1160
BspANI	GGCC	2 cut(s)	826, 1304
BspCNI	CTCAG	4 cut(s)	6, 745, 1558, 1657
BspFNI	CGCG	1 cut(s)	74
BspHI	TCATGA	1 cut(s)	1483
BspLI	GGNNCC	2 cut(s)	171, 1521
BspMAI	CTGCAG	1 cut(s)	119
BspOI	GCTAGC	1 cut(s)	1157
BspPI	GGATC	2 cut(s)	685, 840
BspT104I	TTCGAA	2 cut(s)	38, 571
BspTI	CTTAAG	1 cut(s)	928
BsrDI	GCAATG	3 cut(s)	335, 1143, 1657
BsrFI	RCCGGY	2 cut(s)	172, 1270
BsrI	ACTGG	2 cut(s)	1214, 1550
BssAI	RCCGGY	2 cut(s)	172, 1270
BssECI	CCNNGG	4 cut(s)	302, 303, 828, 1066
BssMI	GATC	5 cut(s)	6, 156, 677, 832, 1635
BssNI	GRCGYC	1 cut(s)	150
BssSI	CACGAG	1 cut(s)	91
BssT1I	CCWWGG	1 cut(s)	828
Bst2BI	CACGAG	1 cut(s)	91
Bst2UI	CCWGG	6 cut(s)	516, 627, 798, 1068, 1296, 1731
Bst4CI	ACNGT	4 cut(s)	358, 559, 1000, 1406
Bst6I	CTCTTC	1 cut(s)	336
BstACI	GRCGYC	1 cut(s)	150
BstAFI	CTTAAG	1 cut(s)	928
BstAPI	GCANNNNNTGC	1 cut(s)	1668
BstBAI	YACGTR	1 cut(s)	1589
BstBI	TTCGAA	2 cut(s)	38, 571
BstC8I	GCNNGC	7 cut(s)	60, 729, 1018, 1096, 1151, 1155, 1669
BstDEI	CTNAG	5 cut(s)	14, 732, 1566, 1644, 1686
BstF5I	GGATG	5 cut(s)	319, 480, 964, 1091, 1219
BstFNI	CGCG	1 cut(s)	74
BstH2I	RGCGCY	1 cut(s)	1017
BstHHI	GCGC	2 cut(s)	1016, 1711
BstKTI	GATC	5 cut(s)	9, 159, 680, 835, 1638
BstMAI	GTCTC	1 cut(s)	328
BstMBI	GATC	5 cut(s)	6, 156, 677, 832, 1635
BstMCI	CGRYCG	1 cut(s)	1271
BstMWI	GCNNNNNNNGC	9 cut(s)	173, 733, 823, 1095, 1163, 1310, 1630, 1659, 1668
BstNI	CCWGG	6 cut(s)	516, 627, 798, 1068, 1296, 1731
BstNSI	RCATGY	2 cut(s)	731, 1153
BstSCI	CCNGG	8 cut(s)	302, 303, 514, 625, 796, 1066, 1294, 1729
BstSFI	CTRYAG	1 cut(s)	115
BstUI	CGCG	1 cut(s)	74
BstV1I	GCAGC	4 cut(s)	399, 511, 1144, 1330
BstV2I	GAAGAC	1 cut(s)	845
BsuRI	GGCC	2 cut(s)	826, 1304
BtgZI	GCGATG	1 cut(s)	1686
BtsCI	GGATG	5 cut(s)	319, 480, 964, 1091, 1219
BtsI	GCAGTG	2 cut(s)	124, 980
BtsIMutI	CAGTG	5 cut(s)	124, 555, 980, 1402, 1557
Cac8I	GCNNGC	7 cut(s)	60, 729, 1018, 1096, 1151, 1155, 1669
CaiI	CAGNNNCTG	1 cut(s)	803
CciI	TCATGA	1 cut(s)	1483
CfoI	GCGC	2 cut(s)	1016, 1711
Cfr10I	RCCGGY	2 cut(s)	172, 1270
Cfr13I	GGNCC	5 cut(s)	170, 825, 1519, 1562, 1727
Cfr9I	CCCGGG	1 cut(s)	303
CseI	GACGC	1 cut(s)	63
CsiI	ACCWGGT	1 cut(s)	796
Csp6I	GTAC	4 cut(s)	46, 467, 862, 1109
CviAII	CATG	8 cut(s)	10, 401, 728, 868, 1150, 1361, 1484, 1490
CviQI	GTAC	4 cut(s)	46, 467, 862, 1109
DdeI	CTNAG	5 cut(s)	14, 732, 1566, 1644, 1686
DpnI	GATC	5 cut(s)	8, 158, 679, 834, 1637
DpnII	GATC	5 cut(s)	6, 156, 677, 832, 1635
DraI	TTTAAA	1 cut(s)	1104
EaeI	YGGCCR	1 cut(s)	1302
Eam1104I	CTCTTC	1 cut(s)	336
EarI	CTCTTC	1 cut(s)	336
Eco130I	CCWWGG	1 cut(s)	828
Eco24I	GRGCYC	1 cut(s)	310
Eco47I	GGWCC	4 cut(s)	170, 1519, 1562, 1727
Eco57I	CTGAAG	4 cut(s)	282, 684, 1376, 1646
Eco72I	CACGTG	1 cut(s)	1589
Eco88I	CYCGRG	1 cut(s)	303
EcoO109I	RGGNCCY	1 cut(s)	1519
EcoRI	GAATTC	3 cut(s)	40, 944, 1225
EcoRII	CCWGG	6 cut(s)	514, 625, 796, 1066, 1294, 1729
EcoT14I	CCWWGG	1 cut(s)	828
EcoT22I	ATGCAT	1 cut(s)	406
EcoT38I	GRGCYC	1 cut(s)	310
ErhI	CCWWGG	1 cut(s)	828
FaeI	CATG	8 cut(s)	13, 404, 731, 871, 1153, 1364, 1487, 1493
FaqI	GGGAC	3 cut(s)	183, 344, 1009
FatI	CATG	8 cut(s)	9, 400, 727, 867, 1149, 1360, 1483, 1489
FauI	CCCGC	3 cut(s)	160, 333, 639
FauNDI	CATATG	2 cut(s)	844, 937
Fnu4HI	GCNGC	6 cut(s)	56, 189, 413, 525, 1158, 1344
FokI	GGATG	5 cut(s)	326, 487, 951, 1098, 1226
FriOI	GRGCYC	1 cut(s)	310
Fsp4HI	GCNGC	6 cut(s)	56, 189, 413, 525, 1158, 1344
FspBI	CTAG	4 cut(s)	528, 659, 986, 1154
GlaI	GCGC	2 cut(s)	1015, 1710
GluI	GCNGC	6 cut(s)	56, 189, 413, 525, 1158, 1344
GsaI	CCCAGC	1 cut(s)	821
GsuI	CTGGAG	1 cut(s)	1089
HaeII	RGCGCY	1 cut(s)	1017
HaeIII	GGCC	2 cut(s)	826, 1304
HapII	CCGG	3 cut(s)	173, 304, 1271
HgaI	GACGC	1 cut(s)	63
HhaI	GCGC	2 cut(s)	1016, 1711
Hin1I	GRCGYC	1 cut(s)	150
Hin1II	CATG	8 cut(s)	13, 404, 731, 871, 1153, 1364, 1487, 1493
Hin6I	GCGC	2 cut(s)	1014, 1709
HinP1I	GCGC	2 cut(s)	1014, 1709
HincII	GTYRAC	1 cut(s)	1543
HindII	GTYRAC	1 cut(s)	1543
HinfI	GANTC	7 cut(s)	134, 344, 397, 545, 756, 808, 1568
HpaII	CCGG	3 cut(s)	173, 304, 1271
HphI	GGTGA	3 cut(s)	339, 346, 1496
Hpy166II	GTNNAC	4 cut(s)	122, 1543, 1594, 1704
Hpy188I	TCNGA	6 cut(s)	373, 688, 741, 837, 1268, 1647
Hpy188III	TCNNGA	8 cut(s)	25, 78, 154, 311, 949, 1484, 1565, 1572
Hpy8I	GTNNAC	4 cut(s)	122, 1543, 1594, 1704
Hpy99I	CGWCG	2 cut(s)	152, 155
HpyAV	CCTTC	8 cut(s)	478, 604, 659, 715, 1025, 1510, 1517, 1709
HpyCH4III	ACNGT	4 cut(s)	358, 559, 1000, 1406
HpyCH4IV	ACGT	2 cut(s)	150, 1588
HpyCH4V	TGCA	8 cut(s)	117, 404, 971, 1136, 1394, 1586, 1633, 1662
HpyF10VI	GCNNNNNNNGC	9 cut(s)	173, 733, 823, 1095, 1163, 1310, 1630, 1659, 1668
HpyF3I	CTNAG	5 cut(s)	14, 732, 1566, 1644, 1686
HpySE526I	ACGT	2 cut(s)	150, 1588
Hsp92I	GRCGYC	1 cut(s)	150
Hsp92II	CATG	8 cut(s)	13, 404, 731, 871, 1153, 1364, 1487, 1493
HspAI	GCGC	2 cut(s)	1014, 1709
Kzo9I	GATC	5 cut(s)	6, 156, 677, 832, 1635
LmnI	GCTCC	3 cut(s)	257, 1073, 1470
Lsp1109I	GCAGC	4 cut(s)	399, 511, 1144, 1330
LweI	GCATC	5 cut(s)	745, 970, 1076, 1175, 1204
MabI	ACCWGGT	1 cut(s)	796
MaeI	CTAG	4 cut(s)	528, 659, 986, 1154
MaeII	ACGT	2 cut(s)	150, 1588
MaeIII	GTNAC	4 cut(s)	345, 384, 649, 1569
MalI	GATC	5 cut(s)	8, 158, 679, 834, 1637
MboI	GATC	5 cut(s)	6, 156, 677, 832, 1635
MboII	GAAGA	8 cut(s)	353, 360, 503, 850, 953, 995, 1172, 1253
MfeI	CAATTG	1 cut(s)	206
MhlI	GDGCHC	3 cut(s)	274, 310, 1258
MlsI	TGGCCA	1 cut(s)	1304
MluNI	TGGCCA	1 cut(s)	1304
MlyI	GAGTC	3 cut(s)	143, 353, 1577
MmeI	TCCRAC	2 cut(s)	1291, 1478
Mox20I	TGGCCA	1 cut(s)	1304
Mph1103I	ATGCAT	1 cut(s)	406
MroXI	GAANNNNTTC	1 cut(s)	618
MscI	TGGCCA	1 cut(s)	1304
MseI	TTAA	9 cut(s)	426, 455, 929, 957, 1103, 1119, 1220, 1349, 1413
MslI	CAYNNNNRTG	1 cut(s)	1488
Msp20I	TGGCCA	1 cut(s)	1304
MspA1I	CMGCKG	1 cut(s)	1624
MspCI	CTTAAG	1 cut(s)	928
MspI	CCGG	3 cut(s)	173, 304, 1271
MspR9I	CCNGG	8 cut(s)	304, 305, 516, 627, 798, 1068, 1296, 1731
MunI	CAATTG	1 cut(s)	206
Mva1269I	GAATGC	1 cut(s)	567
MvaI	CCWGG	6 cut(s)	516, 627, 798, 1068, 1296, 1731
MvnI	CGCG	1 cut(s)	74
MwoI	GCNNNNNNNGC	9 cut(s)	173, 733, 823, 1095, 1163, 1310, 1630, 1659, 1668
NciI	CCSGG	2 cut(s)	304, 305
NdeI	CATATG	2 cut(s)	844, 937
NdeII	GATC	5 cut(s)	6, 156, 677, 832, 1635
NheI	GCTAGC	1 cut(s)	1153
NlaIII	CATG	8 cut(s)	13, 404, 731, 871, 1153, 1364, 1487, 1493
NlaIV	GGNNCC	2 cut(s)	171, 1521
NmuCI	GTSAC	3 cut(s)	345, 649, 1569
NsiI	ATGCAT	1 cut(s)	406
NspI	RCATGY	2 cut(s)	731, 1153
NspV	TTCGAA	2 cut(s)	38, 571
PaeI	GCATGC	2 cut(s)	731, 1153
PagI	TCATGA	1 cut(s)	1483
PcsI	WCGNNNNNNNCGW	2 cut(s)	156, 1264
PctI	GAATGC	1 cut(s)	567
PdmI	GAANNNNTTC	1 cut(s)	618
PfeI	GAWTC	4 cut(s)	397, 545, 756, 808
PkrI	GCNGC	6 cut(s)	57, 190, 414, 526, 1159, 1345
PleI	GAGTC	3 cut(s)	142, 352, 1576
PmaCI	CACGTG	1 cut(s)	1589
PmlI	CACGTG	1 cut(s)	1589
PpsI	GAGTC	3 cut(s)	142, 352, 1576
Ppu21I	YACGTR	1 cut(s)	1589
PpuMI	RGGWCCY	1 cut(s)	1519
Psp5II	RGGWCCY	1 cut(s)	1519
Psp6I	CCWGG	6 cut(s)	514, 625, 796, 1066, 1294, 1729
PspCI	CACGTG	1 cut(s)	1589
PspFI	CCCAGC	1 cut(s)	817
PspGI	CCWGG	6 cut(s)	514, 625, 796, 1066, 1294, 1729
PspN4I	GGNNCC	2 cut(s)	171, 1521
PspPI	GGNCC	5 cut(s)	170, 825, 1519, 1562, 1727
PspPPI	RGGWCCY	1 cut(s)	1519
PstI	CTGCAG	1 cut(s)	119
PstNI	CAGNNNCTG	1 cut(s)	803
PvuII	CAGCTG	1 cut(s)	1624
RsaI	GTAC	4 cut(s)	47, 468, 863, 1110
RsaNI	GTAC	4 cut(s)	46, 467, 862, 1109
RseI	CAYNNNNRTG	1 cut(s)	1488
SaqAI	TTAA	9 cut(s)	426, 455, 929, 957, 1103, 1119, 1220, 1349, 1413
SatI	GCNGC	6 cut(s)	56, 189, 413, 525, 1158, 1344
Sau3AI	GATC	5 cut(s)	6, 156, 677, 832, 1635
Sau96I	GGNCC	5 cut(s)	170, 825, 1519, 1562, 1727
SchI	GAGTC	3 cut(s)	143, 353, 1577
ScrFI	CCNGG	8 cut(s)	304, 305, 516, 627, 798, 1068, 1296, 1731
SduI	GDGCHC	3 cut(s)	274, 310, 1258
SexAI	ACCWGGT	1 cut(s)	796
SfaNI	GCATC	5 cut(s)	745, 970, 1076, 1175, 1204
SfcI	CTRYAG	1 cut(s)	115
SfuI	TTCGAA	2 cut(s)	38, 571
SinI	GGWCC	4 cut(s)	170, 1519, 1562, 1727
SmaI	CCCGGG	1 cut(s)	305
SmiMI	CAYNNNNRTG	1 cut(s)	1488
SmlI	CTYRAG	3 cut(s)	712, 928, 1192
SmoI	CTYRAG	3 cut(s)	712, 928, 1192
SphI	GCATGC	2 cut(s)	731, 1153
SsiI	CCGC	9 cut(s)	55, 66, 72, 125, 167, 188, 326, 646, 1160
SspMI	CTAG	4 cut(s)	528, 659, 986, 1154
StyD4I	CCNGG	8 cut(s)	302, 303, 514, 625, 796, 1066, 1294, 1729
StyI	CCWWGG	1 cut(s)	828
TaaI	ACNGT	4 cut(s)	358, 559, 1000, 1406
TaiI	ACGT	2 cut(s)	153, 1591
TaqI	TCGA	4 cut(s)	5, 38, 571, 673
TauI	GCSGC	2 cut(s)	58, 191
TfiI	GAWTC	4 cut(s)	397, 545, 756, 808
Tru1I	TTAA	9 cut(s)	426, 455, 929, 957, 1103, 1119, 1220, 1349, 1413
Tru9I	TTAA	9 cut(s)	426, 455, 929, 957, 1103, 1119, 1220, 1349, 1413
TscAI	CASTG	5 cut(s)	124, 562, 987, 1409, 1557
TseFI	GTSAC	3 cut(s)	345, 649, 1569
TseI	GCWGC	4 cut(s)	412, 524, 1157, 1343
Tsp45I	GTSAC	3 cut(s)	345, 649, 1569
TspGWI	ACGGA	1 cut(s)	1273
TspMI	CCCGGG	1 cut(s)	303
TspRI	CASTG	5 cut(s)	124, 562, 987, 1409, 1557
Vha464I	CTTAAG	1 cut(s)	928
VpaK11BI	GGWCC	4 cut(s)	170, 1519, 1562, 1727
XapI	RAATTY	6 cut(s)	34, 40, 616, 944, 953, 1225
XceI	RCATGY	2 cut(s)	731, 1153
XmaI	CCCGGG	1 cut(s)	303
XmnI	GAANNNNTTC	1 cut(s)	618
XspI	CTAG	4 cut(s)	528, 659, 986, 1154
ZraI	GACGTC	1 cut(s)	151
Zsp2I	ATGCAT	1 cut(s)	406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.