Rroxscaffold_5G00351230

Electron transfer flavoprotein-ubiquinone oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005790
Physical Location & Seq
Forward (+)
27263263 .. 27271993
8731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351230.1

Sequence Viewer

Length: 1242 bp
ATGTCGATCATGCTGAGGCATATCCTGAAACGAAATTCGAATTCGTACATTGGGCGGCTGGCTTCTGCTTCCGCGTCTGGACGGCTATTCTCGTGGAGGAGGAGGAGGAGGACTTTCTGCAGTGAACCGCAGAGAGAGTCTATTCAATACGACGTCGTGATCGTTGGGGCGGGACCGGCTGGTTTATCGGCGGCGATTAGGTTCAAACAATTGTGCCGTGAAAATGATACTGATTTATCGGTCTGCGTTGTGGAGAAAGGAGCTGAAGTCGGTGCTCACATTATTTCTGGTAATGTCTTTGAACCCCGGGCTCTGGATGAACTTCTCCCACAATGGAGACAGGAAGAGTCACCCATCACCGTCCCTGTTTCTTCTGACAAGTTTTTGTTACTTACAAAGAATCATGCATTTTCGCTTCCTTGTCCTTTTAATAACAGAGGAAACTATGTCATAAGTTTAAGTCAGTTAGTACGATGGATGGGAGTGAAGGCAGAAGAATTGGGAGTAGAAATATATCCAGGCTTTGCTGCTAGTGAGAAAATAATGGATAAATACAACTTGAGAGAGAAGGGGCATGCTCAGCATCAGACTTATGCTTTGGGAATCAAAGAGGTTTGGGAAATTGATGAGAGAGAAAACACAAACCAGATTTCTATTGGCTTTGTGGTTGCTTTGAATTATCAAAACCCTTTCTTAAGTCCATATGAAGAATTCCAGAAATTTAAGCATCATCCTGCAATCAAGCCACTGCTAGAGGGTGGGACTGTTCTTCAATATGGCGCTCGCACCTTGAATGAAGGTGGCTTTCAGATGAAGCATCTAGCTGCAAGGGATTTGGAAGAGATGTTCTTGGAATTGGAGAAACTAAATAAAGCTGGAAGGTGCTTGTTTGTTTCACATGAGGGGATAGGCATGCTAGCTGCGGAAGCATCGTTTGGTGTTCTTCATAAAGGCTCAAGTATGGAAAAATACTGGGATGCTTTAAGGAATTCGTGGATATGGGAAGAATTATATAGAGCACGGAACTTCCGACCGGCATTTGAGTATGGACTTATTCCTGGCTTGGCCATAAGTGCGTTAGAACACTACATAATGAAAGGGAGAGCTGCCTTAACGCTGAAGCATGGAAAACCTGATAATGAAGCAACAAATGTTGCAGAGTTCCACTGTCCAATTAAATATCCAAAGCCAGATGGGGTTTTATCTTTTGATATTCCGACCTCCCTACACAGGTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004174 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005759 GO:0006082 GO:0006091 GO:0006520 GO:0006551 GO:0006552 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009055 GO:0009056 GO:0009062 GO:0009063 GO:0009081 GO:0009083 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009646 GO:0009987 GO:0015980 GO:0016020 GO:0016021 GO:0016042 GO:0016054 GO:0016491 GO:0016645 GO:0016649 GO:0016722 GO:0017133 GO:0019395 GO:0019752 GO:0019866 GO:0022900 GO:0022904 GO:0030258 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0032592 GO:0032787 GO:0032991 GO:0033539 GO:0034440 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043783 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045251 GO:0045333 GO:0046395 GO:0048037 GO:0048038 GO:0048039 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0055114 GO:0070013 GO:0071704 GO:0072329 GO:0097159 GO:0098573 GO:0098798 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ETF_QO FAD_binding_2 NAD_binding_8

Protein Analysis

413

Amino Acids

46.75

Weight (kDa)

8.4

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETFQO_UQ-bd PF21162 212 - 264 2.7e-19 ETF-QO, ubiquinone-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 156
AccII CGCG 1 cut(s) 74
AciI CCGC 6 cut(s) 55, 72, 128, 170, 191, 923
AcoI YGGCCR 1 cut(s) 1065
AcsI RAATTY 5 cut(s) 34, 40, 710, 719, 988
AcuI CTGAAG 2 cut(s) 285, 1139
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 2 cut(s) 47, 471
AfiI CCNNNNNNNGG 2 cut(s) 313, 1230
AflII CTTAAG 1 cut(s) 694
AgsI TTSAA 6 cut(s) 146, 205, 302, 676, 773, 793
AjnI CCWGG 2 cut(s) 517, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AloI GAACNNNNNNTCC 2 cut(s) 830, 862
AluBI AGCT 5 cut(s) 263, 824, 875, 920, 1106
AluI AGCT 5 cut(s) 263, 824, 875, 920, 1106
Alw21I GWGCWC 2 cut(s) 277, 1021
Alw26I GTCTC 1 cut(s) 331
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 1 cut(s) 1065
ApeKI GCWGC 4 cut(s) 527, 824, 920, 1106
ApoI RAATTY 5 cut(s) 34, 40, 710, 719, 988
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 2 cut(s) 307, 308
AsuHPI GGTGA 2 cut(s) 342, 349
AsuII TTCGAA 1 cut(s) 38
AsuNHI GCTAGC 1 cut(s) 916
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 1 cut(s) 173
BalI TGGCCA 1 cut(s) 1067
BanII GRGCYC 1 cut(s) 313
BauI CACGAG 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 277, 1021
BbvCI CCTCAGC 1 cut(s) 14
BbvI GCAGC 4 cut(s) 514, 811, 907, 1093
BccI CCATC 4 cut(s) 362, 468, 472, 1187
BceAI ACGGC 2 cut(s) 98, 201
BciT130I CCWGG 2 cut(s) 519, 1059
BcnI CCSGG 2 cut(s) 307, 308
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 4 cut(s) 531, 752, 821, 917
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 783
BfrI CTTAAG 1 cut(s) 694
BisI GCNGC 6 cut(s) 56, 192, 528, 825, 921, 1107
BlpI GCTNAGC 1 cut(s) 579
BlsI GCNGC 6 cut(s) 57, 193, 529, 826, 922, 1108
Bme1390I CCNGG 4 cut(s) 307, 308, 519, 1059
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 4 cut(s) 307, 308, 519, 1059
BmrI ACTGGG 1 cut(s) 982
BmsI GCATC 5 cut(s) 592, 736, 826, 938, 967
BmtI GCTAGC 1 cut(s) 920
BmuI ACTGGG 1 cut(s) 982
Bpu10I CCTNAGC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 2 cut(s) 580, 940
BpuMI CCSGG 2 cut(s) 307, 308
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 305, 306
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 1230
Bse118I RCCGGY 2 cut(s) 175, 1033
Bse1I ACTGG 1 cut(s) 977
BseBI CCWGG 2 cut(s) 519, 1059
BseDI CCNNGG 2 cut(s) 305, 306
BseGI GGATG 4 cut(s) 322, 483, 730, 982
BseLI CCNNNNNNNGG 2 cut(s) 313, 1230
BseMII CTCAG 2 cut(s) 5, 593
BseNI ACTGG 1 cut(s) 977
BseRI GAGGAG 4 cut(s) 112, 115, 118, 121
BseXI GCAGC 4 cut(s) 514, 811, 907, 1093
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 1034
BshFI GGCC 1 cut(s) 1067
BsiEI CGRYCG 1 cut(s) 1034
BsiHKAI GWGCWC 2 cut(s) 277, 1021
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 3 cut(s) 176, 307, 1034
BslFI GGGAC 3 cut(s) 186, 347, 775
BslI CCNNNNNNNGG 2 cut(s) 313, 1230
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 3 cut(s) 186, 347, 775
BsnI GGCC 1 cut(s) 1067
BsoBI CYCGRG 1 cut(s) 306
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 3 cut(s) 277, 313, 1021
Bsp143I GATC 2 cut(s) 6, 159
Bsp1720I GCTNAGC 1 cut(s) 579
BspACI CCGC 6 cut(s) 55, 72, 128, 170, 191, 923
BspANI GGCC 1 cut(s) 1067
BspCNI CTCAG 2 cut(s) 6, 592
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 920
BspT104I TTCGAA 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 694
BsrFI RCCGGY 2 cut(s) 175, 1033
BsrI ACTGG 1 cut(s) 977
BssAI RCCGGY 2 cut(s) 175, 1033
BssECI CCNNGG 2 cut(s) 305, 306
BssMI GATC 2 cut(s) 6, 159
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 91
Bst2BI CACGAG 1 cut(s) 91
Bst2UI CCWGG 2 cut(s) 519, 1059
Bst4CI ACNGT 3 cut(s) 361, 766, 1169
Bst6I CTCTTC 2 cut(s) 339, 834
BstACI GRCGYC 1 cut(s) 153
BstAFI CTTAAG 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 5 cut(s) 60, 576, 784, 914, 918
BstDEI CTNAG 2 cut(s) 14, 579
BstF5I GGATG 4 cut(s) 322, 483, 730, 982
BstFNI CGCG 1 cut(s) 74
BstH2I RGCGCY 1 cut(s) 783
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 2 cut(s) 9, 162
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 2 cut(s) 6, 159
BstMCI CGRYCG 1 cut(s) 1034
BstMWI GCNNNNNNNGC 4 cut(s) 176, 580, 926, 1073
BstNI CCWGG 2 cut(s) 519, 1059
BstNSI RCATGY 2 cut(s) 578, 916
BstSCI CCNGG 4 cut(s) 305, 306, 517, 1057
BstSFI CTRYAG 1 cut(s) 118
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 4 cut(s) 514, 811, 907, 1093
BsuRI GGCC 1 cut(s) 1067
BtsCI GGATG 4 cut(s) 322, 483, 730, 982
BtsI GCAGTG 2 cut(s) 127, 746
BtsIMutI CAGTG 3 cut(s) 127, 746, 1165
Cac8I GCNNGC 5 cut(s) 60, 576, 784, 914, 918
CfoI GCGC 1 cut(s) 782
Cfr10I RCCGGY 2 cut(s) 175, 1033
Cfr13I GGNCC 1 cut(s) 173
Cfr9I CCCGGG 1 cut(s) 306
CseI GACGC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 46, 470
CviAII CATG 6 cut(s) 10, 404, 575, 899, 913, 1124
CviQI GTAC 2 cut(s) 46, 470
DdeI CTNAG 2 cut(s) 14, 579
DpnI GATC 2 cut(s) 8, 161
DpnII GATC 2 cut(s) 6, 159
EaeI YGGCCR 1 cut(s) 1065
Eam1104I CTCTTC 2 cut(s) 339, 834
EarI CTCTTC 2 cut(s) 339, 834
Eco24I GRGCYC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 285, 1139
Eco88I CYCGRG 1 cut(s) 306
EcoRI GAATTC 3 cut(s) 40, 710, 988
EcoRII CCWGG 2 cut(s) 517, 1057
EcoT22I ATGCAT 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 313
FaeI CATG 6 cut(s) 13, 407, 578, 902, 916, 1127
FaqI GGGAC 3 cut(s) 186, 347, 775
FatI CATG 6 cut(s) 9, 403, 574, 898, 912, 1123
FauI CCCGC 1 cut(s) 163
FauNDI CATATG 1 cut(s) 703
Fnu4HI GCNGC 6 cut(s) 56, 192, 528, 825, 921, 1107
FokI GGATG 4 cut(s) 329, 490, 717, 989
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 6 cut(s) 56, 192, 528, 825, 921, 1107
FspBI CTAG 4 cut(s) 531, 752, 821, 917
GlaI GCGC 1 cut(s) 781
GluI GCNGC 6 cut(s) 56, 192, 528, 825, 921, 1107
HaeII RGCGCY 1 cut(s) 783
HaeIII GGCC 1 cut(s) 1067
HapII CCGG 3 cut(s) 176, 307, 1034
HgaI GACGC 1 cut(s) 63
HhaI GCGC 1 cut(s) 782
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 6 cut(s) 13, 407, 578, 902, 916, 1127
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HinfI GANTC 4 cut(s) 137, 347, 400, 603
HpaII CCGG 3 cut(s) 176, 307, 1034
HphI GGTGA 2 cut(s) 342, 349
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 5 cut(s) 376, 588, 810, 1031, 1218
Hpy188III TCNNGA 5 cut(s) 25, 78, 157, 314, 715
Hpy8I GTNNAC 1 cut(s) 125
Hpy99I CGWCG 2 cut(s) 155, 158
HpyAV CCTTC 4 cut(s) 481, 562, 791, 873
HpyCH4III ACNGT 3 cut(s) 361, 766, 1169
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 5 cut(s) 120, 407, 737, 827, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 580, 926, 1073
HpyF3I CTNAG 2 cut(s) 14, 579
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 6 cut(s) 13, 407, 578, 902, 916, 1127
HspAI GCGC 1 cut(s) 780
Kzo9I GATC 2 cut(s) 6, 159
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 4 cut(s) 514, 811, 907, 1093
LweI GCATC 5 cut(s) 592, 736, 826, 938, 967
MaeI CTAG 4 cut(s) 531, 752, 821, 917
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 2 cut(s) 348, 387
MalI GATC 2 cut(s) 8, 161
MboI GATC 2 cut(s) 6, 159
MboII GAAGA 8 cut(s) 356, 363, 506, 719, 761, 851, 935, 1016
MfeI CAATTG 1 cut(s) 209
MhlI GDGCHC 3 cut(s) 277, 313, 1021
MlsI TGGCCA 1 cut(s) 1067
MluNI TGGCCA 1 cut(s) 1067
MlyI GAGTC 2 cut(s) 146, 356
MmeI TCCRAC 2 cut(s) 1054, 1241
Mox20I TGGCCA 1 cut(s) 1067
Mph1103I ATGCAT 1 cut(s) 409
MscI TGGCCA 1 cut(s) 1067
MseI TTAA 7 cut(s) 429, 458, 695, 723, 983, 1112, 1176
Msp20I TGGCCA 1 cut(s) 1067
MspCI CTTAAG 1 cut(s) 694
MspI CCGG 3 cut(s) 176, 307, 1034
MspR9I CCNGG 4 cut(s) 307, 308, 519, 1059
MunI CAATTG 1 cut(s) 209
MvaI CCWGG 2 cut(s) 519, 1059
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 4 cut(s) 176, 580, 926, 1073
NciI CCSGG 2 cut(s) 307, 308
NdeI CATATG 1 cut(s) 703
NdeII GATC 2 cut(s) 6, 159
NheI GCTAGC 1 cut(s) 916
NlaIII CATG 6 cut(s) 13, 407, 578, 902, 916, 1127
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 348
NsiI ATGCAT 1 cut(s) 409
NspI RCATGY 2 cut(s) 578, 916
NspV TTCGAA 1 cut(s) 38
PaeI GCATGC 2 cut(s) 578, 916
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 1027
PfeI GAWTC 2 cut(s) 400, 603
PkrI GCNGC 6 cut(s) 57, 193, 529, 826, 922, 1108
PleI GAGTC 2 cut(s) 145, 355
PpsI GAGTC 2 cut(s) 145, 355
Psp6I CCWGG 2 cut(s) 517, 1057
PspGI CCWGG 2 cut(s) 517, 1057
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 173
PstI CTGCAG 1 cut(s) 122
RsaI GTAC 2 cut(s) 47, 471
RsaNI GTAC 2 cut(s) 46, 470
SaqAI TTAA 7 cut(s) 429, 458, 695, 723, 983, 1112, 1176
SatI GCNGC 6 cut(s) 56, 192, 528, 825, 921, 1107
Sau3AI GATC 2 cut(s) 6, 159
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 2 cut(s) 146, 356
ScrFI CCNGG 4 cut(s) 307, 308, 519, 1059
SduI GDGCHC 3 cut(s) 277, 313, 1021
SfaNI GCATC 5 cut(s) 592, 736, 826, 938, 967
SfcI CTRYAG 1 cut(s) 118
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 173
SmaI CCCGGG 1 cut(s) 308
SmlI CTYRAG 3 cut(s) 559, 694, 955
SmoI CTYRAG 3 cut(s) 559, 694, 955
SphI GCATGC 2 cut(s) 578, 916
SsiI CCGC 6 cut(s) 55, 72, 128, 170, 191, 923
SspMI CTAG 4 cut(s) 531, 752, 821, 917
StyD4I CCNGG 4 cut(s) 305, 306, 517, 1057
TaaI ACNGT 3 cut(s) 361, 766, 1169
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 5, 38
TaqII GACCGA 1 cut(s) 229
TauI GCSGC 2 cut(s) 58, 194
TfiI GAWTC 2 cut(s) 400, 603
Tru1I TTAA 7 cut(s) 429, 458, 695, 723, 983, 1112, 1176
Tru9I TTAA 7 cut(s) 429, 458, 695, 723, 983, 1112, 1176
TscAI CASTG 3 cut(s) 127, 753, 1172
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 4 cut(s) 527, 824, 920, 1106
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 7 cut(s) 333, 720, 810, 827, 935, 1109, 1155
TspGWI ACGGA 1 cut(s) 1036
TspMI CCCGGG 1 cut(s) 306
TspRI CASTG 3 cut(s) 127, 753, 1172
Vha464I CTTAAG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 5 cut(s) 34, 40, 710, 719, 988
XceI RCATGY 2 cut(s) 578, 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 653
XmaI CCCGGG 1 cut(s) 306
XspI CTAG 4 cut(s) 531, 752, 821, 917
ZraI GACGTC 1 cut(s) 154
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.