pycom04g03770

Electron transfer flavoprotein-ubiquinone oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005790
Physical Location & Seq
Forward (+)
3370016 .. 3378483
8468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03770.17

Sequence Viewer

Length: 1914 bp
ATGATCAGGCTCATAACAAAATCCAAATCTTTCAGGAAACCAAGCTGCTCATCCTCATTTCTATCTTCAATCTCTCCCAGTCCATCTAACCCAGTTTCCTCTTCGCCAAAAACAACCCATATACCAAATGGCGGCTATCTCTACGTTCAGCTTCTGGGTCGAAATTTTGCTTCTTTACGATCCCAGATTGGTTCCCGGCCGGAAGTCTCATCTGGGTATTGCCAAAATTGGGTAAATGGTCCGGCAAATGGGACTTTGGGGTCTCAGACCAGGAGTTTGTGCAGTGAACCGGACAGAGAGTCTATTCAATATGATGTTGTCATTGTTGGGGCTGGACCTGCCGGTCTATCGGCGGCGATAAGGATGAAGCAGTTGTGCCGCGAGAAGGATGTTGATTTGTCAGTGTGTGTTGTGGAGAAAGGAGCTGAAGTTGGTGCTCACATTATTTCTGGTAATGTTTTTGAGCCCCGGGCACTGAATGAACTTCTCCCACAATGGAAAGATGAAGAGTCACCAATCACTGTACCTGTTACTTCTGACAAGTTTTGGCTTCTTACAAAGGATCGTGCACTTTCACTGCCATCTCCTTTTGATAATAGGGGAAACTATGTTATAAGTTTAAGTGAGTTGGTACGATGGATGGGAGTGAAGGCAGAAGATTTGGGAGTAGAAATATATCCTGGCTTTGCTGCTAGTGAGATATTATATGATACAAACGACACGGTTGTTGGCATTGGAACTAATGATATGGGAATTGCCAAAGATGGTTCAAAGAAGGAAAATTTCCAGCGTGGCATAGAATTGAAAGGGCGAGTAACACTTCTAGCTGAAGGGTGTCGAGGATCATTATCAGAGCAAATAATGAAAAAGTACAACTTGAAAGAGAAGGGGAATTTGCAACATCAGACCTATGCCTTGGGAATTAAAGAGGTTTGGGAAATTGATGAAGGAAAGCATGAGCCCGGTGCTGTGTTGCACACTCTGGGTTGGCCCCTGGATCCAAAGACATACGGGGGTTCATTTTTATACCACATGAAAGATAGACAGATTTCTATTGGCTTTGTGGTTGCTTTGAATTATCATAACCCTTTCTTGAACCCTTATGAAGAATTCCAGAAATTCAAGCATCATCCTGCAATCAAAACACTGCTGGAAGGTGGGACTGTTCTTCAATATGGGGCTCGCACCTTGAATGAGGGTGGTTTTCAGTCTATCCCATATCCAGTTTTCCCTGGAGGAGCACTTATTGGATGTTCAGCTGGCTTTTTAAATGTACCCAAAATAAAAGGAACTCATACTGCAATGAAATCAGGCATGCTAGCTGCAGAAGCTACATTCAGTCTACTTCATGAAGGCTTAAGCATGGAAAAATATTGGGATGCTTTAAGGAATTCTTGGATATGGGAAGAACTGTACAGAGCTCGGAACTTCCGACCAGCATTCGAATATGGACTTATTCCTGGCATGGCCTTAAGTGCTCTAGAACATTACATAATGAAAGGAAGATCTCCCTGGACGCTGAAGCATGGAAAACCAGACCATGAAGCAACAAATGTTGCACAGTTCCACTCTCCAATTCAATATCCAAAGCCAGATGGGGTTTTATCTTTCGATATTCTAACCTCCTTACACAGGAGCAACACAAATCATGAACATGACCAACCACCTCACCTTCGGTTGAGAGACCCCAAGATTCCTGAACTTGTAAATTTGCCAGAGTATGCTGGCCCTGAGTCACGATACTGTCCTGCACGTGTATACGAGTATGTCCCTGATGAAGAGGGTCAGCCGAAGTTGCAGATCAATGCTCAGAATTGCCTACATTGCAAGGCATGCGATATCAAAGACCCGAAGCAAAACATTAACTGGACTCCACCGGAAGGAGGTGGCGGTCCAGGCTACTCCATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004174 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005759 GO:0006082 GO:0006091 GO:0006520 GO:0006551 GO:0006552 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009055 GO:0009056 GO:0009062 GO:0009063 GO:0009081 GO:0009083 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009646 GO:0009987 GO:0015980 GO:0016020 GO:0016021 GO:0016042 GO:0016054 GO:0016491 GO:0016645 GO:0016649 GO:0016722 GO:0017133 GO:0019395 GO:0019752 GO:0019866 GO:0022900 GO:0022904 GO:0030258 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0032592 GO:0032787 GO:0032991 GO:0033539 GO:0034440 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043783 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045251 GO:0045333 GO:0046395 GO:0048037 GO:0048038 GO:0048039 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051539 GO:0051540 GO:0055114 GO:0070013 GO:0071704 GO:0072329 GO:0097159 GO:0098573 GO:0098798 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ETF_QO FAD_binding_2 NAD_binding_8

Protein Analysis

638

Amino Acids

70.62

Weight (kDa)

7.05

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 614
AasI GACNNNNNNGTC 2 cut(s) 259, 342
Acc36I ACCTGC 1 cut(s) 346
AccI GTMKAC 2 cut(s) 1342, 1758
AccII CGCG 1 cut(s) 381
AciI CCGC 4 cut(s) 132, 353, 379, 1890
AclWI GGATC 5 cut(s) 174, 570, 850, 992, 1005
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 7 cut(s) 163, 781, 892, 1109, 1118, 1390, 1708
AcuI CTGAAG 3 cut(s) 447, 849, 1541
AcvI CACGTG 1 cut(s) 1754
AfaI GTAC 5 cut(s) 525, 633, 872, 1275, 1415
AfiI CCNNNNNNNGG 7 cut(s) 131, 229, 248, 385, 1632, 1880, 1883
AflII CTTAAG 2 cut(s) 1357, 1471
AflIII ACRYGT 1 cut(s) 1753
AhdI GACNNNNNGTC 1 cut(s) 298
AjnI CCWGG 7 cut(s) 269, 679, 993, 1231, 1459, 1511, 1894
AluBI AGCT 8 cut(s) 45, 151, 425, 827, 1259, 1322, 1331, 1421
AluI AGCT 8 cut(s) 45, 151, 425, 827, 1259, 1322, 1331, 1421
Alw21I GWGCWC 5 cut(s) 439, 571, 1243, 1423, 1480
Alw26I GTCTC 3 cut(s) 211, 267, 1677
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 5 cut(s) 174, 570, 850, 992, 1005
AlwNI CAGNNNCTG 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 4 cut(s) 197, 989, 1467, 1726
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 3 cut(s) 45, 689, 1322
ApoI RAATTY 7 cut(s) 163, 781, 892, 1109, 1118, 1390, 1708
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AspS9I GGNCC 5 cut(s) 239, 335, 990, 1727, 1892
AsuC2I CCSGG 4 cut(s) 196, 469, 470, 963
AsuHPI GGTGA 2 cut(s) 504, 1661
AsuII TTCGAA 1 cut(s) 1443
AsuNHI GCTAGC 1 cut(s) 1318
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 3 cut(s) 239, 335, 1892
BaeGI GKGCMC 2 cut(s) 475, 571
BamHI GGATCC 1 cut(s) 997
BanII GRGCYC 4 cut(s) 468, 963, 1183, 1423
BbrPI CACGTG 1 cut(s) 1754
Bbv12I GWGCWC 5 cut(s) 439, 571, 1243, 1423, 1480
BbvI GCAGC 3 cut(s) 32, 676, 1309
BccI CCATC 6 cut(s) 91, 589, 630, 634, 758, 1589
BciT130I CCWGG 7 cut(s) 271, 681, 995, 1233, 1461, 1513, 1896
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 196, 469, 470, 963
BcoDI GTCTC 3 cut(s) 211, 267, 1677
BfaI CTAG 4 cut(s) 693, 824, 1319, 1481
BfmI CTRYAG 1 cut(s) 1323
BfrI CTTAAG 2 cut(s) 1357, 1471
BfuAI ACCTGC 1 cut(s) 346
BglII AGATCT 1 cut(s) 1505
BisI GCNGC 6 cut(s) 46, 133, 354, 379, 690, 1323
BlsI GCNGC 6 cut(s) 47, 134, 355, 380, 691, 1324
Bme18I GGWCC 3 cut(s) 239, 335, 1892
BmeRI GACNNNNNGTC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 468
BmgT120I GGNCC 5 cut(s) 239, 335, 990, 1727, 1892
BmiI GGNNCC 3 cut(s) 193, 992, 999
BmrI ACTGGG 2 cut(s) 72, 86
BmsI GCATC 2 cut(s) 1135, 1369
BmtI GCTAGC 1 cut(s) 1322
BmuI ACTGGG 2 cut(s) 72, 86
BpmI CTGGAG 1 cut(s) 1254
Bpu14I TTCGAA 1 cut(s) 1443
BpuMI CCSGG 4 cut(s) 196, 469, 470, 963
BsaAI YACGTR 1 cut(s) 1754
BsaBI GATNNNNATC 1 cut(s) 847
BsaI GGTCTC 2 cut(s) 267, 1677
BsaJI CCNNGG 6 cut(s) 467, 468, 915, 993, 1231, 1511
BsaWI WCCGGW 2 cut(s) 289, 1876
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 229, 248, 385, 1632, 1880, 1883
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 4 cut(s) 78, 92, 1223, 1871
Bse3DI GCAATG 2 cut(s) 1308, 1822
Bse8I GATNNNNATC 1 cut(s) 847
BseBI CCWGG 7 cut(s) 271, 681, 995, 1233, 1461, 1513, 1896
BseDI CCNNGG 6 cut(s) 467, 468, 915, 993, 1231, 1511
BseGI GGATG 7 cut(s) 50, 369, 394, 645, 1129, 1256, 1384
BseJI GATNNNNATC 1 cut(s) 847
BseLI CCNNNNNNNGG 7 cut(s) 131, 229, 248, 385, 1632, 1880, 1883
BseMI GCAATG 2 cut(s) 1308, 1822
BseMII CTCAG 3 cut(s) 278, 1722, 1823
BseNI ACTGG 4 cut(s) 78, 92, 1223, 1871
BseRI GAGGAG 1 cut(s) 1251
BseSI GKGCMC 2 cut(s) 475, 571
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 3 cut(s) 32, 676, 1309
BsgI GTGCAG 2 cut(s) 301, 1734
Bsh1236I CGCG 1 cut(s) 381
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 4 cut(s) 199, 991, 1469, 1728
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 5 cut(s) 439, 571, 1243, 1423, 1480
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 8 cut(s) 196, 200, 242, 290, 342, 469, 963, 1877
BslFI GGGAC 3 cut(s) 265, 1174, 1754
BslI CCNNNNNNNGG 7 cut(s) 131, 229, 248, 385, 1632, 1880, 1883
BsmAI GTCTC 3 cut(s) 211, 267, 1677
BsmFI GGGAC 3 cut(s) 265, 1174, 1754
BsmI GAATGC 1 cut(s) 1439
BsnI GGCC 4 cut(s) 199, 991, 1469, 1728
Bso31I GGTCTC 2 cut(s) 267, 1677
BsoBI CYCGRG 1 cut(s) 468
Bsp119I TTCGAA 1 cut(s) 1443
Bsp1286I GDGCHC 9 cut(s) 439, 468, 475, 571, 963, 1183, 1243, 1423, 1480
Bsp1407I TGTACA 1 cut(s) 1413
Bsp143I GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
BspACI CCGC 4 cut(s) 132, 353, 379, 1890
BspANI GGCC 4 cut(s) 199, 991, 1469, 1728
BspCNI CTCAG 3 cut(s) 277, 1723, 1822
BspFNI CGCG 1 cut(s) 381
BspHI TCATGA 2 cut(s) 1348, 1648
BspLI GGNNCC 3 cut(s) 193, 992, 999
BspMAI CTGCAG 1 cut(s) 1327
BspMI ACCTGC 1 cut(s) 346
BspOI GCTAGC 1 cut(s) 1322
BspPI GGATC 5 cut(s) 174, 570, 850, 992, 1005
BspT104I TTCGAA 1 cut(s) 1443
BspTI CTTAAG 2 cut(s) 1357, 1471
BspTNI GGTCTC 2 cut(s) 267, 1677
BsrDI GCAATG 2 cut(s) 1308, 1822
BsrFI RCCGGY 1 cut(s) 341
BsrGI TGTACA 1 cut(s) 1413
BsrI ACTGG 4 cut(s) 78, 92, 1223, 1871
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 6 cut(s) 467, 468, 915, 993, 1231, 1511
BssMI GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
BssNAI GTATAC 1 cut(s) 1759
BssT1I CCWWGG 1 cut(s) 915
Bst1107I GTATAC 1 cut(s) 1759
Bst2UI CCWGG 7 cut(s) 271, 681, 995, 1233, 1461, 1513, 1896
Bst4CI ACNGT 6 cut(s) 523, 724, 1165, 1413, 1563, 1745
Bst6I CTCTTC 3 cut(s) 106, 501, 1773
BstAFI CTTAAG 2 cut(s) 1357, 1471
BstAPI GCANNNNNTGC 1 cut(s) 1833
BstAUI TGTACA 1 cut(s) 1413
BstBAI YACGTR 1 cut(s) 1754
BstBI TTCGAA 1 cut(s) 1443
BstC8I GCNNGC 6 cut(s) 1183, 1261, 1316, 1320, 1726, 1834
BstDEI CTNAG 3 cut(s) 264, 1731, 1809
BstENI CCTNNNNNAGG 1 cut(s) 1630
BstF5I GGATG 7 cut(s) 50, 369, 394, 645, 1129, 1256, 1384
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 7 cut(s) 6, 182, 565, 845, 1000, 1508, 1803
BstMAI GTCTC 3 cut(s) 211, 267, 1677
BstMBI GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 7 cut(s) 338, 1328, 1475, 1795, 1824, 1833, 1896
BstNI CCWGG 7 cut(s) 271, 681, 995, 1233, 1461, 1513, 1896
BstNSI RCATGY 2 cut(s) 1318, 1836
BstSFI CTRYAG 1 cut(s) 1323
BstSLI GKGCMC 2 cut(s) 475, 571
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 32, 676, 1309
BstX2I RGATCY 2 cut(s) 997, 1505
BstYI RGATCY 2 cut(s) 997, 1505
BstZ17I GTATAC 1 cut(s) 1759
BstZI CGGCCG 1 cut(s) 197
BsuRI GGCC 4 cut(s) 199, 991, 1469, 1728
BtsCI GGATG 7 cut(s) 50, 369, 394, 645, 1129, 1256, 1384
BtsI GCAGTG 3 cut(s) 289, 575, 1145
BtsIMutI CAGTG 6 cut(s) 289, 408, 473, 519, 575, 1145
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 6 cut(s) 1183, 1261, 1316, 1320, 1726, 1834
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 2 cut(s) 1348, 1648
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 5 cut(s) 239, 335, 990, 1727, 1892
Cfr9I CCCGGG 1 cut(s) 468
CseI GACGC 1 cut(s) 1525
Csp6I GTAC 5 cut(s) 524, 632, 871, 1274, 1414
CviQI GTAC 5 cut(s) 524, 632, 871, 1274, 1414
DdeI CTNAG 3 cut(s) 264, 1731, 1809
DpnI GATC 7 cut(s) 5, 181, 564, 844, 999, 1507, 1802
DpnII GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
DraI TTTAAA 1 cut(s) 1269
DrdI GACNNNNNNGTC 2 cut(s) 259, 342
DriI GACNNNNNGTC 1 cut(s) 298
DseDI GACNNNNNNGTC 2 cut(s) 259, 342
EaeI YGGCCR 1 cut(s) 197
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 106, 501, 1773
Eam1105I GACNNNNNGTC 1 cut(s) 298
EarI CTCTTC 3 cut(s) 106, 501, 1773
Ecl136II GAGCTC 1 cut(s) 1421
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 4 cut(s) 468, 963, 1183, 1423
Eco31I GGTCTC 2 cut(s) 267, 1677
Eco32I GATATC 1 cut(s) 1840
Eco47I GGWCC 3 cut(s) 239, 335, 1892
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 1421
Eco57I CTGAAG 3 cut(s) 447, 849, 1541
Eco72I CACGTG 1 cut(s) 1754
Eco88I CYCGRG 1 cut(s) 468
EcoICRI GAGCTC 1 cut(s) 1421
EcoNI CCTNNNNNAGG 1 cut(s) 1630
EcoRI GAATTC 2 cut(s) 1109, 1390
EcoRII CCWGG 7 cut(s) 269, 679, 993, 1231, 1459, 1511, 1894
EcoRV GATATC 1 cut(s) 1840
EcoT14I CCWWGG 1 cut(s) 915
EcoT38I GRGCYC 4 cut(s) 468, 963, 1183, 1423
ErhI CCWWGG 1 cut(s) 915
FalI AAGNNNNNCTT 4 cut(s) 860, 892, 1378, 1410
FaqI GGGAC 3 cut(s) 265, 1174, 1754
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 1342, 1758
Fnu4HI GCNGC 6 cut(s) 46, 133, 354, 379, 690, 1323
FokI GGATG 7 cut(s) 37, 376, 401, 652, 1116, 1263, 1391
FriOI GRGCYC 4 cut(s) 468, 963, 1183, 1423
Fsp4HI GCNGC 6 cut(s) 46, 133, 354, 379, 690, 1323
FspBI CTAG 4 cut(s) 693, 824, 1319, 1481
GluI GCNGC 6 cut(s) 46, 133, 354, 379, 690, 1323
GsuI CTGGAG 1 cut(s) 1254
HaeIII GGCC 4 cut(s) 199, 991, 1469, 1728
HapII CCGG 8 cut(s) 196, 200, 242, 290, 342, 469, 963, 1877
HgaI GACGC 1 cut(s) 1525
HinfI GANTC 5 cut(s) 299, 509, 1693, 1733, 1870
HpaII CCGG 8 cut(s) 196, 200, 242, 290, 342, 469, 963, 1877
HphI GGTGA 2 cut(s) 504, 1661
Hpy166II GTNNAC 4 cut(s) 287, 569, 1343, 1759
Hpy188I TCNGA 7 cut(s) 267, 538, 853, 906, 1425, 1433, 1812
Hpy188III TCNNGA 8 cut(s) 34, 1093, 1114, 1349, 1481, 1649, 1697, 1737
Hpy8I GTNNAC 4 cut(s) 287, 569, 1343, 1759
HpyCH4III ACNGT 6 cut(s) 523, 724, 1165, 1413, 1563, 1745
HpyCH4IV ACGT 2 cut(s) 144, 1753
HpyF10VI GCNNNNNNNGC 7 cut(s) 338, 1328, 1475, 1795, 1824, 1833, 1896
HpyF3I CTNAG 3 cut(s) 264, 1731, 1809
HpySE526I ACGT 2 cut(s) 144, 1753
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
LmnI GCTCC 3 cut(s) 422, 1238, 1635
Lsp1109I GCAGC 3 cut(s) 32, 676, 1309
LweI GCATC 2 cut(s) 1135, 1369
MaeI CTAG 4 cut(s) 693, 824, 1319, 1481
MaeII ACGT 2 cut(s) 144, 1753
MaeIII GTNAC 4 cut(s) 510, 529, 814, 1734
MalI GATC 7 cut(s) 5, 181, 564, 844, 999, 1507, 1802
MboI GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
MboII GAAGA 9 cut(s) 57, 93, 518, 668, 1118, 1160, 1418, 1515, 1790
MflI RGATCY 2 cut(s) 997, 1505
MhlI GDGCHC 9 cut(s) 439, 468, 475, 571, 963, 1183, 1243, 1423, 1480
MlyI GAGTC 4 cut(s) 308, 518, 1742, 1864
MmeI TCCRAC 1 cut(s) 1456
MseI TTAA 7 cut(s) 620, 924, 1268, 1358, 1385, 1472, 1863
MslI CAYNNNNRTG 1 cut(s) 1653
MspA1I CMGCKG 1 cut(s) 1259
MspCI CTTAAG 2 cut(s) 1357, 1471
MspI CCGG 8 cut(s) 196, 200, 242, 290, 342, 469, 963, 1877
Mva1269I GAATGC 1 cut(s) 1439
MvaI CCWGG 7 cut(s) 271, 681, 995, 1233, 1461, 1513, 1896
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 7 cut(s) 338, 1328, 1475, 1795, 1824, 1833, 1896
NciI CCSGG 4 cut(s) 196, 469, 470, 963
NdeII GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
NheI GCTAGC 1 cut(s) 1318
NlaIV GGNNCC 3 cut(s) 193, 992, 999
NmuCI GTSAC 2 cut(s) 510, 1734
NspI RCATGY 2 cut(s) 1318, 1836
NspV TTCGAA 1 cut(s) 1443
PaeI GCATGC 2 cut(s) 1318, 1836
PagI TCATGA 2 cut(s) 1348, 1648
PcsI WCGNNNNNNNCGW 1 cut(s) 1429
PctI GAATGC 1 cut(s) 1439
PfeI GAWTC 1 cut(s) 1693
PkrI GCNGC 6 cut(s) 47, 134, 355, 380, 691, 1324
PleI GAGTC 4 cut(s) 307, 517, 1741, 1864
PmaCI CACGTG 1 cut(s) 1754
PmlI CACGTG 1 cut(s) 1754
PpsI GAGTC 4 cut(s) 307, 517, 1741, 1864
Ppu21I YACGTR 1 cut(s) 1754
PsiI TTATAA 1 cut(s) 614
Psp124BI GAGCTC 1 cut(s) 1423
Psp6I CCWGG 7 cut(s) 269, 679, 993, 1231, 1459, 1511, 1894
PspCI CACGTG 1 cut(s) 1754
PspGI CCWGG 7 cut(s) 269, 679, 993, 1231, 1459, 1511, 1894
PspN4I GGNNCC 3 cut(s) 193, 992, 999
PspPI GGNCC 5 cut(s) 239, 335, 990, 1727, 1892
PstI CTGCAG 1 cut(s) 1327
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 2 cut(s) 997, 1505
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 5 cut(s) 525, 633, 872, 1275, 1415
RsaNI GTAC 5 cut(s) 524, 632, 871, 1274, 1414
RseI CAYNNNNRTG 1 cut(s) 1653
SacI GAGCTC 1 cut(s) 1423
SaqAI TTAA 7 cut(s) 620, 924, 1268, 1358, 1385, 1472, 1863
SatI GCNGC 6 cut(s) 46, 133, 354, 379, 690, 1323
Sau3AI GATC 7 cut(s) 3, 179, 562, 842, 997, 1505, 1800
Sau96I GGNCC 5 cut(s) 239, 335, 990, 1727, 1892
SchI GAGTC 4 cut(s) 308, 518, 1742, 1864
SduI GDGCHC 9 cut(s) 439, 468, 475, 571, 963, 1183, 1243, 1423, 1480
SfaNI GCATC 2 cut(s) 1135, 1369
SfcI CTRYAG 1 cut(s) 1323
SfuI TTCGAA 1 cut(s) 1443
SinI GGWCC 3 cut(s) 239, 335, 1892
SmaI CCCGGG 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 1653
SmlI CTYRAG 2 cut(s) 1357, 1471
SmoI CTYRAG 2 cut(s) 1357, 1471
SphI GCATGC 2 cut(s) 1318, 1836
SsiI CCGC 4 cut(s) 132, 353, 379, 1890
SspI AATATT 1 cut(s) 1373
SspMI CTAG 4 cut(s) 693, 824, 1319, 1481
SstI GAGCTC 1 cut(s) 1423
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 6 cut(s) 523, 724, 1165, 1413, 1563, 1745
TaiI ACGT 2 cut(s) 147, 1756
TaqI TCGA 4 cut(s) 160, 838, 1443, 1611
TatI WGTACW 2 cut(s) 870, 1413
TauI GCSGC 3 cut(s) 135, 356, 381
TfiI GAWTC 1 cut(s) 1693
Tru1I TTAA 7 cut(s) 620, 924, 1268, 1358, 1385, 1472, 1863
Tru9I TTAA 7 cut(s) 620, 924, 1268, 1358, 1385, 1472, 1863
TscAI CASTG 6 cut(s) 289, 408, 480, 526, 582, 1152
TseFI GTSAC 2 cut(s) 510, 1734
TseI GCWGC 3 cut(s) 45, 689, 1322
Tsp45I GTSAC 2 cut(s) 510, 1734
TspMI CCCGGG 1 cut(s) 468
TspRI CASTG 6 cut(s) 289, 408, 480, 526, 582, 1152
Vha464I CTTAAG 2 cut(s) 1357, 1471
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 3 cut(s) 239, 335, 1892
XagI CCTNNNNNAGG 1 cut(s) 1630
XapI RAATTY 7 cut(s) 163, 781, 892, 1109, 1118, 1390, 1708
XbaI TCTAGA 1 cut(s) 1480
XceI RCATGY 2 cut(s) 1318, 1836
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmaI CCCGGG 1 cut(s) 468
XmiI GTMKAC 2 cut(s) 1342, 1758
XspI CTAG 4 cut(s) 693, 824, 1319, 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.