MD04G1050800.v1.1

taxadien-5-alpha-ol O-acetyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014513
Physical Location & Seq
Forward (+)
5973156 .. 5974779
1624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1050800.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGAAATGTCTAAAGGCGCCAATGATCTCCTCCCAGAAATTCACATTCAGTCTGTGCAGACCGTGACACCAACGAAGGTAACCGATCCACGGCAAGTGCGCCAAGTGTCAGCGGTAGATCCTATAGGTTTTGGAATATTCCGGAAGTGTCTTCACATCCTTCTTTACCACGCAAAGGCAACCGAGGACGGTGCTCGTTGGTTTCACGTTGGATGGCTCAGGGAGTCTCTAGCAAGGGCCTTGCTGGAGCAACCGTTAATAGCAGGGCGATTTCGACAAGCTTTTGATCGTCAGAATGAGGAAGGAAATCAACTGGAGATTGTGTCAAATGATAGTGGCGTTAGACTTTTTCAGACAAGCATCCCGATAACATTGTCTGAGTTTCTTGATTTGAAGGGCAGCAGAGACGGAGAGGATGCGGAAGCTAAGCTTGTGTTTTGGAAAGATATTGATGAACAAAATCCTCAGTACTCTCCACTTGTTTATGTTCAGGTGACCAAATTCCAATGTGGAGGATGCTCAGTTGGAATTAGTTGCAGCATTCTTCTGGCAGATATTTTGTTGAAGGAAAACTTCTTAAAGAAATGGGCTAAAATTCACAACAATCTGCTTTCAAAAGATGATCTACCTGCAGCACCAATGTATTATATACCTGATATTATGAAATCTACTGGTTGCTCCCCCACCAGCATATTTAGCTCTAATCCTAGCAACAAATGTGGCCAAACTGTTATTTTCACTATCTCTGCGGAGACTGTAAATCCATTGAAGGATGATGCATTGCAGCTTTGTGTTGAAGAAGCAGAGAACAAATACGGTAACAATATGGCTCCCAACTTTCCCATGTTTTTGAAGACCAACTACCGCAATGCAATCAAGGTTGAGAAGTTTCCAAAAGATGGACTTATTAAGCCGAAACGGCTAGGTAGTCGGAGTCAGCTTTCTAGAGCAGTGTGGGATGATTTTGGAGCAAATGAGATCGAATTTCGCGAAGGGAATAAGCCGGAATATGTTTCATATTGGGTTGGATTCGTTTCTGGTGGACTTGTCATGGCAATGCCATCGGCTGATCGAAGGGGCTCTCTCAATGAAGTGAAAGTAGTGATTGTCACAGTTCCTATTGAGAAGGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

379

Amino Acids

42.34

Weight (kDa)

6.27

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 14 - 200 5.4e-18 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02890
fragaria_vesca FvH4_5g20090
malus_domestica MD04G1050800.v1.1
prunus_persica Prupe.5G059300_v2.0.a1
pyrus_communis pycom04g04470
rosa_chinensis RchiOBHm_Chr7g0204981
rosa_laevigata RLG00000003418
rosa_multiflora Rmu_sc0005112.1_g000025 Rmu_sc0024226.1_g000001
rosa_roxburghii Rroxscaffold_3G00252630
rosa_rugosa Rorug07G0086800
rosa_samantha Rh7AG217800 Rh7BG214700 Rh7CG230800 Rh7CG230900 Rh7DG225100
rosa_wichuraiana Rw7G018870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 17
AccB7I CCANNNNNTGG 1 cut(s) 899
AccII CGCG 1 cut(s) 990
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 4 cut(s) 113, 419, 749, 865
AclWI GGATC 2 cut(s) 80, 113
AcoI YGGCCR 1 cut(s) 721
AcsI RAATTY 4 cut(s) 39, 500, 594, 983
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 3 cut(s) 90, 175, 899
AgsI TTSAA 6 cut(s) 394, 565, 615, 769, 797, 853
AluBI AGCT 6 cut(s) 281, 425, 430, 699, 787, 940
AluI AGCT 6 cut(s) 281, 425, 430, 699, 787, 940
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 3 cut(s) 231, 399, 746
AlwI GGATC 2 cut(s) 80, 113
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 2 cut(s) 237, 721
ApeKI GCWGC 4 cut(s) 399, 537, 632, 784
ApoI RAATTY 4 cut(s) 39, 500, 594, 983
AspLEI GCGC 2 cut(s) 20, 102
AspS9I GGNCC 1 cut(s) 237
AsuHPI GGTGA 1 cut(s) 505
BalI TGGCCA 1 cut(s) 723
BanI GGYRCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 1082
BarI GAAGNNNNNNTAC 2 cut(s) 845, 877
BbsI GAAGAC 2 cut(s) 143, 860
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 4 cut(s) 411, 549, 644, 796
BccI CCATC 3 cut(s) 207, 893, 1069
BceAI ACGGC 2 cut(s) 107, 935
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 1044, 1078
BcoDI GTCTC 3 cut(s) 231, 399, 746
BfaI CTAG 4 cut(s) 230, 708, 923, 945
BfmI CTRYAG 2 cut(s) 123, 630
BfoI RGCGCY 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 637
BglI GCCNNNNNGGC 1 cut(s) 919
BisI GCNGC 4 cut(s) 400, 538, 633, 785
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 4 cut(s) 401, 539, 634, 786
BmcAI AGTACT 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 237
BmiI GGNNCC 2 cut(s) 19, 831
BmsI GCATC 4 cut(s) 369, 406, 506, 766
BpiI GAAGAC 2 cut(s) 143, 860
BpmI CTGGAG 2 cut(s) 266, 335
Bpu10I CCTNAGC 1 cut(s) 218
Bpu1102I GCTNAGC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 89, 183
BsaWI WCCGGW 1 cut(s) 141
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 308, 338
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 175, 899
Bse1I ACTGG 2 cut(s) 318, 676
Bse3DI GCAATG 3 cut(s) 779, 874, 1062
BseAI TCCGGA 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 89, 183
BseGI GGATG 7 cut(s) 156, 218, 360, 421, 521, 778, 964
BseLI CCNNNNNNNGG 3 cut(s) 90, 175, 899
BseMI GCAATG 3 cut(s) 779, 874, 1062
BseMII CTCAG 4 cut(s) 232, 369, 479, 534
BseNI ACTGG 2 cut(s) 318, 676
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 411, 549, 644, 796
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 2 cut(s) 239, 723
BshNI GGYRCC 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 196
BsiSI CCGG 2 cut(s) 142, 1004
BslI CCNNNNNNNGG 3 cut(s) 90, 175, 899
BsmAI GTCTC 3 cut(s) 231, 399, 746
BsmBI CGTCTC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 2 cut(s) 239, 723
Bsp1286I GDGCHC 2 cut(s) 196, 1082
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
Bsp1720I GCTNAGC 1 cut(s) 426
Bsp68I TCGCGA 1 cut(s) 990
BspACI CCGC 4 cut(s) 113, 419, 749, 865
BspANI GGCC 2 cut(s) 239, 723
BspCNI CTCAG 4 cut(s) 231, 370, 478, 533
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 1 cut(s) 990
BspLI GGNNCC 2 cut(s) 19, 831
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 80, 113
BspT107I GGYRCC 1 cut(s) 17
BsrDI GCAATG 3 cut(s) 779, 874, 1062
BsrI ACTGG 2 cut(s) 318, 676
BssECI CCNNGG 2 cut(s) 89, 183
BssMI GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
BssNI GRCGYC 1 cut(s) 18
Bst4CI ACNGT 7 cut(s) 64, 191, 255, 730, 757, 818, 1114
BstACI GRCGYC 1 cut(s) 18
BstDEI CTNAG 5 cut(s) 218, 378, 426, 465, 520
BstDSI CCRYGG 1 cut(s) 89
BstEII GGTNACC 2 cut(s) 79, 493
BstF5I GGATG 7 cut(s) 156, 218, 360, 421, 521, 778, 964
BstFNI CGCG 1 cut(s) 990
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 2 cut(s) 20, 102
BstKTI GATC 7 cut(s) 28, 88, 121, 289, 625, 981, 1072
BstMAI GTCTC 3 cut(s) 231, 399, 746
BstMBI GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
BstMWI GCNNNNNNNGC 2 cut(s) 696, 919
BstPI GGTNACC 2 cut(s) 79, 493
BstSFI CTRYAG 2 cut(s) 123, 630
BstUI CGCG 1 cut(s) 990
BstV1I GCAGC 4 cut(s) 411, 549, 644, 796
BstV2I GAAGAC 2 cut(s) 143, 860
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 2 cut(s) 239, 723
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 7 cut(s) 156, 218, 360, 421, 521, 778, 964
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 1 cut(s) 957
BtuMI TCGCGA 1 cut(s) 990
BveI ACCTGC 1 cut(s) 637
CfoI GCGC 2 cut(s) 20, 102
Cfr13I GGNCC 1 cut(s) 237
Csp6I GTAC 1 cut(s) 469
CspCI CAANNNNNGTGG 2 cut(s) 700, 735
CviAII CATG 2 cut(s) 844, 1051
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 5 cut(s) 218, 378, 426, 465, 520
DinI GGCGCC 1 cut(s) 19
DpnI GATC 7 cut(s) 27, 87, 120, 288, 624, 980, 1071
DpnII GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
EaeI YGGCCR 1 cut(s) 721
Eco24I GRGCYC 1 cut(s) 1082
Eco91I GGTNACC 2 cut(s) 79, 493
EcoO109I RGGNCCY 1 cut(s) 237
EcoO65I GGTNACC 2 cut(s) 79, 493
EcoT22I ATGCAT 1 cut(s) 781
EcoT38I GRGCYC 1 cut(s) 1082
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 2 cut(s) 847, 1054
FalI AAGNNNNNCTT 6 cut(s) 414, 446, 557, 589, 888, 920
FatI CATG 2 cut(s) 843, 1050
Fnu4HI GCNGC 4 cut(s) 400, 538, 633, 785
FokI GGATG 7 cut(s) 143, 225, 347, 428, 528, 785, 971
FriOI GRGCYC 1 cut(s) 1082
Fsp4HI GCNGC 4 cut(s) 400, 538, 633, 785
FspBI CTAG 4 cut(s) 230, 708, 923, 945
GlaI GCGC 2 cut(s) 19, 101
GluI GCNGC 4 cut(s) 400, 538, 633, 785
GsuI CTGGAG 2 cut(s) 266, 335
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 2 cut(s) 239, 723
HapII CCGG 2 cut(s) 142, 1004
HhaI GCGC 2 cut(s) 20, 102
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 2 cut(s) 847, 1054
Hin6I GCGC 2 cut(s) 18, 100
HinP1I GCGC 2 cut(s) 18, 100
HindIII AAGCTT 2 cut(s) 279, 428
HinfI GANTC 3 cut(s) 224, 934, 1029
HpaII CCGG 2 cut(s) 142, 1004
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 1 cut(s) 1043
Hpy188I TCNGA 4 cut(s) 294, 354, 379, 933
Hpy188III TCNNGA 5 cut(s) 142, 364, 386, 945, 989
Hpy8I GTNNAC 1 cut(s) 1043
HpyAV CCTTC 9 cut(s) 70, 170, 296, 388, 559, 763, 986, 1068, 1120
HpyCH4III ACNGT 7 cut(s) 64, 191, 255, 730, 757, 818, 1114
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 6 cut(s) 58, 537, 632, 779, 784, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 696, 919
HpyF3I CTNAG 5 cut(s) 218, 378, 426, 465, 520
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 2 cut(s) 847, 1054
HspAI GCGC 2 cut(s) 18, 100
KasI GGCGCC 1 cut(s) 17
Kpn2I TCCGGA 1 cut(s) 141
Kzo9I GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
LmnI GCTCC 4 cut(s) 247, 683, 835, 968
Lsp1109I GCAGC 4 cut(s) 411, 549, 644, 796
LweI GCATC 4 cut(s) 369, 406, 506, 766
MaeI CTAG 4 cut(s) 230, 708, 923, 945
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 5 cut(s) 64, 79, 493, 818, 1108
MalI GATC 7 cut(s) 27, 87, 120, 288, 624, 980, 1071
MboI GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
MboII GAAGA 4 cut(s) 143, 536, 809, 865
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 2 cut(s) 196, 1082
MlsI TGGCCA 1 cut(s) 723
MluCI AATT 5 cut(s) 39, 500, 528, 594, 983
MluNI TGGCCA 1 cut(s) 723
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 2 cut(s) 233, 943
MmeI TCCRAC 4 cut(s) 190, 505, 911, 1006
MnlI CCTC 6 cut(s) 41, 178, 292, 406, 474, 506
Mox20I TGGCCA 1 cut(s) 723
Mph1103I ATGCAT 1 cut(s) 781
MroI TCCGGA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 723
MseI TTAA 3 cut(s) 257, 578, 909
MslI CAYNNNNRTG 1 cut(s) 1055
Msp20I TGGCCA 1 cut(s) 723
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 2 cut(s) 142, 1004
Mva1269I GAATGC 1 cut(s) 540
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 2 cut(s) 696, 919
NarI GGCGCC 1 cut(s) 18
NdeII GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
NlaIII CATG 2 cut(s) 847, 1054
NlaIV GGNNCC 2 cut(s) 19, 831
NmuCI GTSAC 3 cut(s) 64, 493, 1108
NruI TCGCGA 1 cut(s) 990
NsiI ATGCAT 1 cut(s) 781
PcsI WCGNNNNNNNCGW 1 cut(s) 987
PctI GAATGC 1 cut(s) 540
PfeI GAWTC 1 cut(s) 1029
PflMI CCANNNNNTGG 1 cut(s) 899
PkrI GCNGC 4 cut(s) 401, 539, 634, 786
PleI GAGTC 2 cut(s) 232, 942
PluTI GGCGCC 1 cut(s) 21
PpsI GAGTC 2 cut(s) 232, 942
PspEI GGTNACC 2 cut(s) 79, 493
PspN4I GGNNCC 2 cut(s) 19, 831
PspPI GGNCC 1 cut(s) 237
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 118
RruI TCGCGA 1 cut(s) 990
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 1055
SaqAI TTAA 3 cut(s) 257, 578, 909
SatI GCNGC 4 cut(s) 400, 538, 633, 785
Sau3AI GATC 7 cut(s) 25, 85, 118, 286, 622, 978, 1069
Sau96I GGNCC 1 cut(s) 237
ScaI AGTACT 1 cut(s) 470
SchI GAGTC 2 cut(s) 233, 943
SduI GDGCHC 2 cut(s) 196, 1082
SfaNI GCATC 4 cut(s) 369, 406, 506, 766
SfcI CTRYAG 2 cut(s) 123, 630
SfoI GGCGCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 1055
Sse9I AATT 5 cut(s) 39, 500, 528, 594, 983
SsiI CCGC 4 cut(s) 113, 419, 749, 865
SspDI GGCGCC 1 cut(s) 17
SspI AATATT 1 cut(s) 138
SspMI CTAG 4 cut(s) 230, 708, 923, 945
TaaI ACNGT 7 cut(s) 64, 191, 255, 730, 757, 818, 1114
TaiI ACGT 1 cut(s) 210
TaqI TCGA 3 cut(s) 274, 981, 1072
TasI AATT 5 cut(s) 39, 500, 528, 594, 983
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 1 cut(s) 1029
Tru1I TTAA 3 cut(s) 257, 578, 909
Tru9I TTAA 3 cut(s) 257, 578, 909
TscAI CASTG 1 cut(s) 957
TseFI GTSAC 3 cut(s) 64, 493, 1108
TseI GCWGC 4 cut(s) 399, 537, 632, 784
Tsp45I GTSAC 3 cut(s) 64, 493, 1108
TspDTI ATGAA 4 cut(s) 468, 677, 1005, 1104
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 1 cut(s) 957
Van91I CCANNNNNTGG 1 cut(s) 899
XapI RAATTY 4 cut(s) 39, 500, 594, 983
XbaI TCTAGA 1 cut(s) 944
XspI CTAG 4 cut(s) 230, 708, 923, 945
ZrmI AGTACT 1 cut(s) 470
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.