RLG00000003418

taxadien-5-alpha-ol O-acetyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014513
Physical Location & Seq
Reverse (-)
48222555 .. 48223676
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003418

Sequence Viewer

Length: 1041 bp
ATGCTCCTCCCAGAAGCACACATTGAATCCGTCCAGTCTGTAATGCCCTTCAAGATCACTGATCCGCGGCCGGCACGCCAAGTGTCGGCCGCAGACCCCGTCGGTTTGGGAATATTCCAACAGTGCCTGCACATACTTCTCTACTACACAAAAGCAACCCAAGAGGACTCACGTTGGCTTCATGCTGGTTGGATCAAAGAATCACTTTCGAGGGCGTTGCTGGAGCAACCGCTATTTGCTGGCCGTTTACGCATGAGTAATGGGGGAGAGTTGGAGATTGTGGCAAATGACAGCGGCATTAGACTCGTCGAAACTCGTACTCCGATGACCATGGCTGAGTTTCTGTCTTACAAGGACGGCACAGAGGATACAGAAGATAAACTTGTGTTTTGGAAAACTATTGATGAACGAAGTCCTCAGTACTCTCCTCTCTTTTATGTTCAGGTGACATATTTTCAGTGTGGAGGGAGATCAGTAGGAATTAGCTGCAGTGTTCTTCTAGCAGATCTTTTGTTCAAAGAAAATTTTCTCAAGAAATGGGCTAACATCCACCACAATCTGCTTTCAGACGAGTGTCTACCTGCGACACCGTTTTATAACTTCCCACATGTTGAAAAAAGTGGAAATTCCTGCAAGAATATATTTGTCTCTATTCCCAGCAAAAAATGTGGCAAGACAATGATCTTCAATGTTGCAGCTGAGAAGGCGGACTCGGAGAACGATGATGCATTGCAACTTTGTGTTGAAGAAGCAGAGAGCAAGTTTGGTTTTGGGAAATTGTTTGTGAAGATCAACATCTCATCCTGCAGTGCAATCAAGGTTGAGAATTTTCAAAAAGAAGGACTTGCTAAGCCGAAACTGAGCCTAATTGCTGAAGCGGATTGGGATTATTTCGGGGCAAAGGAGGTCGCGTTTCGTGAAGGGAATAAGCCGGCTACTGTTTCGTATTGGTTTGGATTCATTTCTGGTGGACTTGTTATGGCAGTGCCATCATCAGCTGATCAGGAGGGTGCTTCAGTGATTGTCACAGTTCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

347

Amino Acids

38.46

Weight (kDa)

5.52

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 44 - 186 1.1e-21 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02890
fragaria_vesca FvH4_5g20090
malus_domestica MD04G1050800.v1.1
prunus_persica Prupe.5G059300_v2.0.a1
pyrus_communis pycom04g04470
rosa_chinensis RchiOBHm_Chr7g0204981
rosa_laevigata RLG00000003418
rosa_multiflora Rmu_sc0005112.1_g000025 Rmu_sc0024226.1_g000001
rosa_roxburghii Rroxscaffold_3G00252630
rosa_rugosa Rorug07G0086800
rosa_samantha Rh7AG217800 Rh7BG214700 Rh7CG230800 Rh7CG230900 Rh7DG225100
rosa_wichuraiana Rw7G018870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
Acc36I ACCTGC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 577
AccII CGCG 2 cut(s) 67, 911
AciI CCGC 7 cut(s) 65, 67, 90, 230, 294, 707, 878
AclWI GGATC 2 cut(s) 56, 200
AcoI YGGCCR 3 cut(s) 68, 87, 241
AcsI RAATTY 3 cut(s) 523, 625, 826
AcuI CTGAAG 2 cut(s) 894, 999
AfaI GTAC 2 cut(s) 319, 422
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 7 cut(s) 26, 52, 517, 614, 688, 746, 833
AluBI AGCT 3 cut(s) 486, 698, 998
AluI AGCT 3 cut(s) 486, 698, 998
Alw26I GTCTC 1 cut(s) 652
AlwI GGATC 2 cut(s) 56, 200
AlwNI CAGNNNCTG 1 cut(s) 127
AoxI GGCC 3 cut(s) 68, 87, 241
ApeKI GCWGC 2 cut(s) 486, 695
ApoI RAATTY 3 cut(s) 523, 625, 826
Asp700I GAANNNNTTC 1 cut(s) 525
AsuHPI GGTGA 1 cut(s) 457
BbvI GCAGC 2 cut(s) 473, 707
BccI CCATC 1 cut(s) 997
BceAI ACGGC 2 cut(s) 228, 373
BcgI CGANNNNNNTGC 4 cut(s) 199, 233, 286, 320
BciVI GTATCC 1 cut(s) 361
BclI TGATCA 1 cut(s) 1000
BcoDI GTCTC 1 cut(s) 652
BfaI CTAG 1 cut(s) 500
BfmI CTRYAG 2 cut(s) 487, 805
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 1 cut(s) 361
BglII AGATCT 1 cut(s) 505
BisI GCNGC 5 cut(s) 68, 90, 295, 487, 696
BlpI GCTNAGC 1 cut(s) 849
BlsI GCNGC 5 cut(s) 69, 91, 296, 488, 697
BmcAI AGTACT 1 cut(s) 422
BmsI GCATC 1 cut(s) 715
BoxI GACNNNNGTC 1 cut(s) 573
BpmI CTGGAG 1 cut(s) 242
Bpu1102I GCTNAGC 1 cut(s) 849
BpuEI CTTGAG 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 794
BsaJI CCNNGG 2 cut(s) 65, 330
BsaXI ACNNNNNCTCC 4 cut(s) 304, 334, 896, 926
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 2 cut(s) 70, 931
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 728
Bse8I GATNNNNATC 1 cut(s) 794
BseDI CCNNGG 2 cut(s) 65, 330
BseGI GGATG 2 cut(s) 546, 800
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 4 cut(s) 327, 431, 690, 851
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 417
BseX3I CGGCCG 2 cut(s) 68, 87
BseXI GCAGC 2 cut(s) 473, 707
BseYI CCCAGC 1 cut(s) 656
BsgI GTGCAG 1 cut(s) 113
Bsh1236I CGCG 2 cut(s) 67, 911
Bsh1285I CGRYCG 2 cut(s) 71, 90
BshFI GGCC 3 cut(s) 70, 89, 243
BsiEI CGRYCG 2 cut(s) 71, 90
BsiSI CCGG 2 cut(s) 71, 932
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 652
BsnI GGCC 3 cut(s) 70, 89, 243
Bsp143I GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
Bsp1720I GCTNAGC 1 cut(s) 849
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 7 cut(s) 65, 67, 90, 230, 294, 707, 878
BspANI GGCC 3 cut(s) 70, 89, 243
BspCNI CTCAG 4 cut(s) 328, 430, 691, 852
BspFNI CGCG 2 cut(s) 67, 911
BspMAI CTGCAG 2 cut(s) 491, 809
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 2 cut(s) 56, 200
BsrDI GCAATG 1 cut(s) 728
BsrFI RCCGGY 2 cut(s) 70, 931
BsrI ACTGG 1 cut(s) 34
BssAI RCCGGY 2 cut(s) 70, 931
BssECI CCNNGG 2 cut(s) 65, 330
BssMI GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 4 cut(s) 123, 591, 940, 1030
BstC8I GCNNGC 5 cut(s) 72, 76, 128, 241, 933
BstDEI CTNAG 5 cut(s) 336, 417, 699, 849, 860
BstDSI CCRYGG 2 cut(s) 65, 330
BstF5I GGATG 2 cut(s) 546, 800
BstFNI CGCG 2 cut(s) 67, 911
BstKTI GATC 8 cut(s) 57, 64, 195, 473, 508, 684, 792, 1003
BstMAI GTCTC 1 cut(s) 652
BstMBI GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
BstMCI CGRYCG 2 cut(s) 71, 90
BstMWI GCNNNNNNNGC 2 cut(s) 249, 704
BstNSI RCATGY 1 cut(s) 611
BstPAI GACNNNNGTC 1 cut(s) 573
BstSFI CTRYAG 2 cut(s) 487, 805
BstUI CGCG 2 cut(s) 67, 911
BstV1I GCAGC 2 cut(s) 473, 707
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BstZI CGGCCG 2 cut(s) 68, 87
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 3 cut(s) 70, 89, 243
BtgI CCRYGG 2 cut(s) 65, 330
BtsCI GGATG 2 cut(s) 546, 800
BtsI GCAGTG 3 cut(s) 496, 814, 990
BtsIMutI CAGTG 7 cut(s) 57, 128, 464, 496, 814, 990, 1023
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 5 cut(s) 72, 76, 128, 241, 933
CaiI CAGNNNCTG 1 cut(s) 127
Cfr10I RCCGGY 2 cut(s) 70, 931
Cfr42I CCGCGG 1 cut(s) 68
Csp6I GTAC 2 cut(s) 318, 421
CspCI CAANNNNNGTGG 2 cut(s) 649, 684
CviAII CATG 4 cut(s) 182, 253, 331, 608
CviQI GTAC 2 cut(s) 318, 421
DdeI CTNAG 5 cut(s) 336, 417, 699, 849, 860
DpnI GATC 8 cut(s) 56, 63, 194, 472, 507, 683, 791, 1002
DpnII GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
EaeI YGGCCR 3 cut(s) 68, 87, 241
EagI CGGCCG 2 cut(s) 68, 87
EciI GGCGGA 1 cut(s) 722
EclXI CGGCCG 2 cut(s) 68, 87
Eco130I CCWWGG 1 cut(s) 330
Eco52I CGGCCG 2 cut(s) 68, 87
Eco57I CTGAAG 2 cut(s) 894, 999
EcoT14I CCWWGG 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 4 cut(s) 185, 256, 334, 611
FalI AAGNNNNNCTT 6 cut(s) 189, 221, 366, 398, 828, 860
FatI CATG 4 cut(s) 181, 252, 330, 607
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 1 cut(s) 577
Fnu4HI GCNGC 5 cut(s) 68, 90, 295, 487, 696
FokI GGATG 2 cut(s) 533, 787
Fsp4HI GCNGC 5 cut(s) 68, 90, 295, 487, 696
FspBI CTAG 1 cut(s) 500
GluI GCNGC 5 cut(s) 68, 90, 295, 487, 696
GsaI CCCAGC 1 cut(s) 660
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 3 cut(s) 70, 89, 243
HapII CCGG 2 cut(s) 71, 932
Hin1II CATG 4 cut(s) 185, 256, 334, 611
HinfI GANTC 6 cut(s) 26, 167, 200, 303, 710, 957
HpaII CCGG 2 cut(s) 71, 932
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 3 cut(s) 248, 578, 971
Hpy188I TCNGA 3 cut(s) 324, 568, 715
Hpy188III TCNNGA 4 cut(s) 52, 532, 917, 1004
Hpy8I GTNNAC 3 cut(s) 248, 578, 971
Hpy99I CGWCG 2 cut(s) 104, 311
HpyAV CCTTC 4 cut(s) 58, 697, 833, 914
HpyCH4III ACNGT 4 cut(s) 123, 591, 940, 1030
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 8 cut(s) 130, 489, 633, 695, 728, 733, 807, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 704
HpyF3I CTNAG 5 cut(s) 336, 417, 699, 849, 860
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 4 cut(s) 185, 256, 334, 611
KroI GCCGGC 2 cut(s) 70, 931
KroNI GCCGGC 2 cut(s) 72, 933
Ksp22I TGATCA 1 cut(s) 1000
KspI CCGCGG 1 cut(s) 68
Kzo9I GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
LmnI GCTCC 2 cut(s) 9, 223
Lsp1109I GCAGC 2 cut(s) 473, 707
LweI GCATC 1 cut(s) 715
MaeI CTAG 1 cut(s) 500
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 2 cut(s) 445, 1024
MalI GATC 8 cut(s) 56, 63, 194, 472, 507, 683, 791, 1002
MboI GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
MboII GAAGA 5 cut(s) 386, 488, 676, 758, 799
MflI RGATCY 1 cut(s) 505
MluCI AATT 7 cut(s) 480, 523, 625, 776, 826, 867, 1036
MlyI GAGTC 3 cut(s) 161, 297, 704
MmeI TCCRAC 3 cut(s) 142, 170, 252
MnlI CCTC 9 cut(s) 17, 157, 204, 358, 426, 438, 458, 898, 1000
Mph1103I ATGCAT 1 cut(s) 730
MroNI GCCGGC 2 cut(s) 70, 931
MroXI GAANNNNTTC 1 cut(s) 525
MspA1I CMGCKG 4 cut(s) 67, 294, 698, 998
MspI CCGG 2 cut(s) 71, 932
MvnI CGCG 2 cut(s) 67, 911
MwoI GCNNNNNNNGC 2 cut(s) 249, 704
NaeI GCCGGC 2 cut(s) 72, 933
NcoI CCATGG 1 cut(s) 330
NdeII GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
NgoMIV GCCGGC 2 cut(s) 70, 931
NlaIII CATG 4 cut(s) 185, 256, 334, 611
NmuCI GTSAC 2 cut(s) 445, 1024
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 1 cut(s) 611
PciI ACATGT 1 cut(s) 607
PdiI GCCGGC 2 cut(s) 72, 933
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 3 cut(s) 26, 200, 957
PflFI GACNNNGTC 1 cut(s) 98
PkrI GCNGC 5 cut(s) 69, 91, 296, 488, 697
PleI GAGTC 3 cut(s) 161, 297, 704
PpsI GAGTC 3 cut(s) 161, 297, 704
PscI ACATGT 1 cut(s) 607
PshAI GACNNNNGTC 1 cut(s) 573
PsiI TTATAA 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 656
PstI CTGCAG 2 cut(s) 491, 809
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 98
PvuII CAGCTG 2 cut(s) 698, 998
RsaI GTAC 2 cut(s) 319, 422
RsaNI GTAC 2 cut(s) 318, 421
SacII CCGCGG 1 cut(s) 68
SatI GCNGC 5 cut(s) 68, 90, 295, 487, 696
Sau3AI GATC 8 cut(s) 54, 61, 192, 470, 505, 681, 789, 1000
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 3 cut(s) 161, 297, 704
SetI ASST 8 cut(s) 175, 447, 488, 583, 700, 822, 909, 1000
SfaNI GCATC 1 cut(s) 715
SfcI CTRYAG 2 cut(s) 487, 805
Sfr303I CCGCGG 1 cut(s) 68
SgrBI CCGCGG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 7 cut(s) 480, 523, 625, 776, 826, 867, 1036
SsiI CCGC 7 cut(s) 65, 67, 90, 230, 294, 707, 878
SspI AATATT 1 cut(s) 114
SspMI CTAG 1 cut(s) 500
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 4 cut(s) 123, 591, 940, 1030
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 209, 309
TasI AATT 7 cut(s) 480, 523, 625, 776, 826, 867, 1036
TatI WGTACW 1 cut(s) 420
TauI GCSGC 3 cut(s) 70, 92, 297
TfiI GAWTC 3 cut(s) 26, 200, 957
TscAI CASTG 7 cut(s) 64, 128, 464, 496, 814, 990, 1023
TseFI GTSAC 2 cut(s) 445, 1024
TseI GCWGC 2 cut(s) 486, 695
Tsp45I GTSAC 2 cut(s) 445, 1024
TspDTI ATGAA 3 cut(s) 170, 420, 949
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 7 cut(s) 64, 128, 464, 496, 814, 990, 1023
Tth111I GACNNNGTC 1 cut(s) 98
XapI RAATTY 3 cut(s) 523, 625, 826
XceI RCATGY 1 cut(s) 611
XmiI GTMKAC 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 1 cut(s) 500
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.