Rroxscaffold_3G00252630

taxadien-5-alpha-ol O-acetyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014513
Physical Location & Seq
Reverse (-)
46670787 .. 46673091
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252630.1

Sequence Viewer

Length: 1098 bp
ATGGAATCTCCTTCATCAACAGTTCCTCTCAAGATGACATTGTCCAATGGTGTCACCATGCTCCTCCCAGAAGCACACATTGAATCCGTCCAGTCTGTAATGCCCTTCAAGATCACCGATCCGCGGCCGGCACGCCAAGTGTCGGCCACAGACCCCGTCGGTTTGGGAATATTCCAACAGTGCCTGCACATAGTTCTCTACTACACAAAAGCAACCGAAGAGGACTCACGTTGGCTCCATGCTGGTTGGATCAAGGAATCACTTTCGAGGGCTTTGCTGGAGCAACCGCTATTTGCTGGCCGTTTACGCACGAGTGATGGGGGAGAGTTGGAGATTGTGGCAAATGACAGCGGCATTAGACTCGTCGAAACTCGTACTCCGATGACCATGGCTGAGTTTCTGTCTTACAAGGACAGCACGGAGGATACAGAAGATAAACTTGTGTTTTGGAAAACTATTGATGAACGAAGTCCTCAGTACTCTCCTCTCTTTTATGTTCAGGTGACATATTTTCAGTGTGGAGGGAGATCAGTAGGAATTAGCTGCAGTGTTCTTCTAGCAGATCTTTTGTTCAAAGAAAATTTTCTCAAGAAATGGGCTAACATCCACCACAATCTGCTTTCAGACGAGACTCTACCTGCGACACCCTTTTATAACTTCCCACATGTTGAAAAAAGTGGAAATTCCTGCAAGAATATATTTGTCCCTATTCTCAGCAAAAAATGTGGCAAGACAATGATCTTCAATGTTGCAGCTGAGAAGGCGGACTCGGAGAACGATGATGCATTGCAACTTTGTGTTGAAGAAGCAAAGAGCAAGTTTGGTTTTGGGAAATTGTTTGTGAAGATCAACATCTCATCCTGCAGTGCAATCAAGGTTGAGAATTTTCAAAAAGAAGGACTTGCTAAGCCGAAACTGAGCCTAATTGCTGAAGCGGATTGGGATTATTTCGGGGCAAAGGAGGTCGAGTTTCGTGAAGGGAATAAGCCGGTTACTGTTTCGTATTGGTTTGGATTCATTTCTGGTGGACTTGTTATGGCAGTGCCATCATCAGCTGATCATGAGGGTGCTTCAGTGATTGTCACAGTTCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

365

Amino Acids

40.56

Weight (kDa)

5.51

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 63 - 205 1.8e-22 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02890
fragaria_vesca FvH4_5g20090
malus_domestica MD04G1050800.v1.1
prunus_persica Prupe.5G059300_v2.0.a1
pyrus_communis pycom04g04470
rosa_chinensis RchiOBHm_Chr7g0204981
rosa_laevigata RLG00000003418
rosa_multiflora Rmu_sc0005112.1_g000025 Rmu_sc0024226.1_g000001
rosa_roxburghii Rroxscaffold_3G00252630
rosa_rugosa Rorug07G0086800
rosa_samantha Rh7AG217800 Rh7BG214700 Rh7CG230800 Rh7CG230900 Rh7DG225100
rosa_wichuraiana Rw7G018870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
Acc36I ACCTGC 1 cut(s) 646
AccII CGCG 1 cut(s) 124
AciI CCGC 6 cut(s) 122, 124, 287, 351, 764, 935
AclWI GGATC 2 cut(s) 113, 257
AcoI YGGCCR 3 cut(s) 125, 144, 298
AcsI RAATTY 3 cut(s) 580, 682, 883
AcuI CTGAAG 2 cut(s) 951, 1056
AfaI GTAC 2 cut(s) 376, 479
AfiI CCNNNNNNNGG 2 cut(s) 123, 142
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 7 cut(s) 83, 109, 574, 671, 745, 803, 890
AluBI AGCT 3 cut(s) 543, 755, 1055
AluI AGCT 3 cut(s) 543, 755, 1055
Alw26I GTCTC 1 cut(s) 623
AlwI GGATC 2 cut(s) 113, 257
AlwNI CAGNNNCTG 1 cut(s) 184
AoxI GGCC 3 cut(s) 125, 144, 298
ApeKI GCWGC 2 cut(s) 543, 752
ApoI RAATTY 3 cut(s) 580, 682, 883
Asp700I GAANNNNTTC 1 cut(s) 582
AsuHPI GGTGA 3 cut(s) 46, 106, 514
BauI CACGAG 1 cut(s) 310
BbvI GCAGC 2 cut(s) 530, 764
BccI CCATC 2 cut(s) 311, 1054
BceAI ACGGC 1 cut(s) 285
BcgI CGANNNNNNTGC 4 cut(s) 256, 290, 343, 377
BciVI GTATCC 1 cut(s) 418
BclI TGATCA 1 cut(s) 1057
BcoDI GTCTC 1 cut(s) 623
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 2 cut(s) 544, 862
BfuAI ACCTGC 1 cut(s) 646
BfuI GTATCC 1 cut(s) 418
BglII AGATCT 1 cut(s) 562
BisI GCNGC 4 cut(s) 125, 352, 544, 753
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 4 cut(s) 126, 353, 545, 754
BmcAI AGTACT 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 236
BmsI GCATC 1 cut(s) 772
BpmI CTGGAG 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 906
BpuEI CTTGAG 2 cut(s) 14, 572
BsaBI GATNNNNATC 1 cut(s) 851
BsaJI CCNNGG 2 cut(s) 122, 387
BsaXI ACNNNNNCTCC 6 cut(s) 219, 249, 361, 391, 953, 983
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 142
Bse118I RCCGGY 2 cut(s) 127, 988
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 785
Bse8I GATNNNNATC 1 cut(s) 851
BseDI CCNNGG 2 cut(s) 122, 387
BseGI GGATG 2 cut(s) 603, 857
BseJI GATNNNNATC 1 cut(s) 851
BseLI CCNNNNNNNGG 2 cut(s) 123, 142
BseMI GCAATG 1 cut(s) 785
BseMII CTCAG 5 cut(s) 384, 488, 727, 747, 908
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 2 cut(s) 53, 474
BseX3I CGGCCG 1 cut(s) 125
BseXI GCAGC 2 cut(s) 530, 764
BsgI GTGCAG 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 124
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 3 cut(s) 127, 146, 300
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 128, 989
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 123, 142
BsmAI GTCTC 1 cut(s) 623
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 3 cut(s) 127, 146, 300
Bsp143I GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
Bsp1720I GCTNAGC 1 cut(s) 906
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 6 cut(s) 122, 124, 287, 351, 764, 935
BspANI GGCC 3 cut(s) 127, 146, 300
BspCNI CTCAG 5 cut(s) 385, 487, 726, 748, 909
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 1 cut(s) 236
BspMAI CTGCAG 2 cut(s) 548, 866
BspMI ACCTGC 1 cut(s) 646
BspPI GGATC 2 cut(s) 113, 257
BsrDI GCAATG 1 cut(s) 785
BsrFI RCCGGY 2 cut(s) 127, 988
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 2 cut(s) 127, 988
BssECI CCNNGG 2 cut(s) 122, 387
BssMI GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
BssSI CACGAG 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 310
Bst4CI ACNGT 4 cut(s) 22, 180, 997, 1087
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 129, 133, 185, 298
BstDEI CTNAG 6 cut(s) 393, 474, 713, 756, 906, 917
BstDSI CCRYGG 2 cut(s) 122, 387
BstF5I GGATG 2 cut(s) 603, 857
BstFNI CGCG 1 cut(s) 124
BstKTI GATC 8 cut(s) 114, 121, 252, 530, 565, 741, 849, 1060
BstMAI GTCTC 1 cut(s) 623
BstMBI GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 2 cut(s) 306, 761
BstNSI RCATGY 1 cut(s) 668
BstSFI CTRYAG 2 cut(s) 544, 862
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 530, 764
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BstZI CGGCCG 1 cut(s) 125
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 3 cut(s) 127, 146, 300
BtgI CCRYGG 2 cut(s) 122, 387
BtsCI GGATG 2 cut(s) 603, 857
BtsI GCAGTG 3 cut(s) 553, 871, 1047
BtsIMutI CAGTG 6 cut(s) 185, 521, 553, 871, 1047, 1080
BveI ACCTGC 1 cut(s) 646
Cac8I GCNNGC 4 cut(s) 129, 133, 185, 298
CaiI CAGNNNCTG 1 cut(s) 184
CciI TCATGA 1 cut(s) 1060
Cfr10I RCCGGY 2 cut(s) 127, 988
Cfr42I CCGCGG 1 cut(s) 125
Csp6I GTAC 2 cut(s) 375, 478
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviAII CATG 5 cut(s) 58, 239, 388, 665, 1061
CviQI GTAC 2 cut(s) 375, 478
DdeI CTNAG 6 cut(s) 393, 474, 713, 756, 906, 917
DpnI GATC 8 cut(s) 113, 120, 251, 529, 564, 740, 848, 1059
DpnII GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
EaeI YGGCCR 3 cut(s) 125, 144, 298
EagI CGGCCG 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 1 cut(s) 779
EclXI CGGCCG 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 387
Eco52I CGGCCG 1 cut(s) 125
Eco57I CTGAAG 2 cut(s) 951, 1056
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 787
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 61, 242, 391, 668, 1064
FalI AAGNNNNNCTT 4 cut(s) 423, 455, 885, 917
FaqI GGGAC 1 cut(s) 689
FatI CATG 5 cut(s) 57, 238, 387, 664, 1060
FbaI TGATCA 1 cut(s) 1057
Fnu4HI GCNGC 4 cut(s) 125, 352, 544, 753
FokI GGATG 2 cut(s) 590, 844
Fsp4HI GCNGC 4 cut(s) 125, 352, 544, 753
FspBI CTAG 1 cut(s) 557
GluI GCNGC 4 cut(s) 125, 352, 544, 753
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 3 cut(s) 127, 146, 300
HapII CCGG 2 cut(s) 128, 989
Hin1II CATG 5 cut(s) 61, 242, 391, 668, 1064
HinfI GANTC 8 cut(s) 5, 83, 224, 257, 360, 631, 767, 1014
HpaII CCGG 2 cut(s) 128, 989
HphI GGTGA 3 cut(s) 46, 106, 514
Hpy166II GTNNAC 2 cut(s) 305, 1028
Hpy188I TCNGA 3 cut(s) 381, 625, 772
Hpy188III TCNNGA 5 cut(s) 31, 109, 589, 974, 1061
Hpy8I GTNNAC 2 cut(s) 305, 1028
Hpy99I CGWCG 2 cut(s) 161, 368
HpyAV CCTTC 5 cut(s) 21, 115, 754, 890, 971
HpyCH4III ACNGT 4 cut(s) 22, 180, 997, 1087
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 8 cut(s) 187, 546, 690, 752, 785, 790, 864, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 761
HpyF3I CTNAG 6 cut(s) 393, 474, 713, 756, 906, 917
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 5 cut(s) 61, 242, 391, 668, 1064
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 1057
KspI CCGCGG 1 cut(s) 125
Kzo9I GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
LmnI GCTCC 3 cut(s) 66, 240, 280
Lsp1109I GCAGC 2 cut(s) 530, 764
LweI GCATC 1 cut(s) 772
MaeI CTAG 1 cut(s) 557
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 4 cut(s) 52, 502, 991, 1081
MalI GATC 8 cut(s) 113, 120, 251, 529, 564, 740, 848, 1059
MboI GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
MboII GAAGA 6 cut(s) 230, 443, 545, 733, 815, 856
MflI RGATCY 1 cut(s) 562
MluCI AATT 7 cut(s) 537, 580, 682, 833, 883, 924, 1093
MlyI GAGTC 4 cut(s) 218, 354, 625, 761
MmeI TCCRAC 3 cut(s) 199, 227, 309
Mph1103I ATGCAT 1 cut(s) 787
MroNI GCCGGC 1 cut(s) 127
MroXI GAANNNNTTC 1 cut(s) 582
MslI CAYNNNNRTG 1 cut(s) 1065
MspA1I CMGCKG 4 cut(s) 124, 351, 755, 1055
MspI CCGG 2 cut(s) 128, 989
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 306, 761
NaeI GCCGGC 1 cut(s) 129
NcoI CCATGG 1 cut(s) 387
NdeII GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
NgoMIV GCCGGC 1 cut(s) 127
NlaIII CATG 5 cut(s) 61, 242, 391, 668, 1064
NlaIV GGNNCC 1 cut(s) 236
NmuCI GTSAC 3 cut(s) 52, 502, 1081
NsiI ATGCAT 1 cut(s) 787
NspI RCATGY 1 cut(s) 668
PagI TCATGA 1 cut(s) 1060
PciI ACATGT 1 cut(s) 664
PdiI GCCGGC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 4 cut(s) 5, 83, 257, 1014
PflFI GACNNNGTC 2 cut(s) 40, 155
PkrI GCNGC 4 cut(s) 126, 353, 545, 754
PleI GAGTC 4 cut(s) 218, 354, 625, 761
PpsI GAGTC 4 cut(s) 218, 354, 625, 761
PscI ACATGT 1 cut(s) 664
PsiI TTATAA 1 cut(s) 654
PspN4I GGNNCC 1 cut(s) 236
PstI CTGCAG 2 cut(s) 548, 866
PstNI CAGNNNCTG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 562
PsyI GACNNNGTC 2 cut(s) 40, 155
PvuII CAGCTG 2 cut(s) 755, 1055
RsaI GTAC 2 cut(s) 376, 479
RsaNI GTAC 2 cut(s) 375, 478
RseI CAYNNNNRTG 1 cut(s) 1065
SacII CCGCGG 1 cut(s) 125
SatI GCNGC 4 cut(s) 125, 352, 544, 753
Sau3AI GATC 8 cut(s) 111, 118, 249, 527, 562, 738, 846, 1057
ScaI AGTACT 1 cut(s) 479
SchI GAGTC 4 cut(s) 218, 354, 625, 761
SetI ASST 8 cut(s) 232, 504, 545, 640, 757, 879, 966, 1057
SfaNI GCATC 1 cut(s) 772
SfcI CTRYAG 2 cut(s) 544, 862
Sfr303I CCGCGG 1 cut(s) 125
SgrBI CCGCGG 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 1065
SmlI CTYRAG 2 cut(s) 29, 587
SmoI CTYRAG 2 cut(s) 29, 587
Sse9I AATT 7 cut(s) 537, 580, 682, 833, 883, 924, 1093
SsiI CCGC 6 cut(s) 122, 124, 287, 351, 764, 935
SspI AATATT 1 cut(s) 171
SspMI CTAG 1 cut(s) 557
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 4 cut(s) 22, 180, 997, 1087
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 266, 366, 966
TasI AATT 7 cut(s) 537, 580, 682, 833, 883, 924, 1093
TatI WGTACW 1 cut(s) 477
TauI GCSGC 2 cut(s) 127, 354
TfiI GAWTC 4 cut(s) 5, 83, 257, 1014
TscAI CASTG 6 cut(s) 185, 521, 553, 871, 1047, 1080
TseFI GTSAC 3 cut(s) 52, 502, 1081
TseI GCWGC 2 cut(s) 543, 752
Tsp45I GTSAC 3 cut(s) 52, 502, 1081
TspDTI ATGAA 2 cut(s) 477, 1006
TspGWI ACGGA 2 cut(s) 76, 434
TspRI CASTG 6 cut(s) 185, 521, 553, 871, 1047, 1080
Tth111I GACNNNGTC 2 cut(s) 40, 155
XapI RAATTY 3 cut(s) 580, 682, 883
XceI RCATGY 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 1 cut(s) 557
ZrmI AGTACT 1 cut(s) 479
Zsp2I ATGCAT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.