Rh7DG225100

taxadien-5-alpha-ol O-acetyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0014513
Physical Location & Seq
Forward (+)
22011170 .. 22012311
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG225100.1

Sequence Viewer

Length: 1062 bp
ATGACATTGTCCAATGGTGTCACCATGCTCCTCCCAGAAGCACACATTGAATCCGTCCAGTCTGTAATGCCCTTCAAGATCACCGATCCGCGGCCGGCACGCCAAGTGTCGGCCGCAGACCCCGTCGGTTTGGGAATATTCCAACAGTGCCTGCACATAGTTCTCTACTACACAAAAGCAACCGAAGAGGACTCACGTTGGCTCCATGCTGGTTGGATCAAGGAATCACTTTCGAGGGCGTTGCTGGAGCAACCGCTATTTGCTGGCCGTTTACGCACGAGTGATGGGGGAGAGTTGGAGATTGTGGCAAATGACAGCGGCGTTAGACTCATCGAAACTCGTACTCCGATGACCATGGCTGAGTTTCTGTCTTACAAGGACAGCACGGAGGATACAGAAGATAAACTTGTGTTTTGGAAAACTATTGATGAACGAAGTCCTCAGTACTCTCCTCTCTTTTATGTTCAGGTGACATATTTTCAGTGTGGAGGGAGATCAGTGGGAATTAGCTGCAGTGTTCTTCTAGCAGATCTTTTGTTCAAAGAAAATTTTCTCAAGAAATGGGCTAACATCCACCACAATCTGCTTTCAGACGAGAGTCTACCTGCGACACCCTTTTATAACTTCCCACATGTTGAAAAAAGTGGAAATCCCTGCAAGAATATATTTGTCTCTATTCCGAGCAAAAAATGTGGCAAGACAATGATCTTCAATGTTGCAGCTGAGAAGGCGGACTCGGAGAACGATGAAGCATTGCAACTTTGTGTTGAAGAAGCAGAGAGCAAGTTTGGTTTTGGGAAATTGTTTGTGAAGATCAACATCTCATCCTGCAGTGCAATCAAGGTTGAGAATTTTCAAAAAGGACTTGCTAAGCCGAAACTGAGCCTAATTGCTGAAGCGGATTGGGATTATTTCGGGGCAAAGGAGGTCGAGTTTCGTGAAGGGAATAAGCCGGCTACTGTTTCGTATTGGTTTGGATTCATTTCTGGTGGACTTGTTATGGCAGTGCCATCATCAGCTGATCAGGAGGGTGCTTCAGTGATTGTCACAGTTCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

353

Amino Acids

39.19

Weight (kDa)

5.33

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 52 - 194 7.2e-23 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02890
fragaria_vesca FvH4_5g20090
malus_domestica MD04G1050800.v1.1
prunus_persica Prupe.5G059300_v2.0.a1
pyrus_communis pycom04g04470
rosa_chinensis RchiOBHm_Chr7g0204981
rosa_laevigata RLG00000003418
rosa_multiflora Rmu_sc0005112.1_g000025 Rmu_sc0024226.1_g000001
rosa_roxburghii Rroxscaffold_3G00252630
rosa_rugosa Rorug07G0086800
rosa_samantha Rh7AG217800 Rh7BG214700 Rh7CG230800 Rh7CG230900 Rh7DG225100
rosa_wichuraiana Rw7G018870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 613
AccI GTMKAC 1 cut(s) 601
AccII CGCG 1 cut(s) 91
AciI CCGC 7 cut(s) 89, 91, 114, 254, 318, 731, 899
AclWI GGATC 2 cut(s) 80, 224
AcoI YGGCCR 3 cut(s) 92, 111, 265
AcsI RAATTY 2 cut(s) 547, 850
AcuI CTGAAG 2 cut(s) 915, 1020
AfaI GTAC 2 cut(s) 343, 446
AfiI CCNNNNNNNGG 2 cut(s) 90, 109
AflIII ACRYGT 1 cut(s) 631
AgsI TTSAA 7 cut(s) 50, 76, 541, 638, 712, 770, 857
AluBI AGCT 3 cut(s) 510, 722, 1019
AluI AGCT 3 cut(s) 510, 722, 1019
Alw26I GTCTC 1 cut(s) 676
AlwI GGATC 2 cut(s) 80, 224
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 3 cut(s) 92, 111, 265
ApeKI GCWGC 2 cut(s) 510, 719
ApoI RAATTY 2 cut(s) 547, 850
Asp700I GAANNNNTTC 1 cut(s) 549
AsuHPI GGTGA 3 cut(s) 13, 73, 481
BauI CACGAG 1 cut(s) 277
BbvI GCAGC 2 cut(s) 497, 731
BccI CCATC 2 cut(s) 278, 1018
BceAI ACGGC 1 cut(s) 252
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 1 cut(s) 1021
BcoDI GTCTC 1 cut(s) 676
BfaI CTAG 1 cut(s) 524
BfmI CTRYAG 2 cut(s) 511, 829
BfuAI ACCTGC 1 cut(s) 613
BfuI GTATCC 1 cut(s) 385
BglII AGATCT 1 cut(s) 529
BisI GCNGC 5 cut(s) 92, 114, 319, 511, 720
BlpI GCTNAGC 1 cut(s) 870
BlsI GCNGC 5 cut(s) 93, 115, 320, 512, 721
BmcAI AGTACT 1 cut(s) 446
BmiI GGNNCC 1 cut(s) 203
BoxI GACNNNNGTC 1 cut(s) 597
BpmI CTGGAG 1 cut(s) 266
Bpu1102I GCTNAGC 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 818
BsaJI CCNNGG 2 cut(s) 89, 354
BsaXI ACNNNNNCTCC 6 cut(s) 186, 216, 328, 358, 917, 947
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 109
Bse118I RCCGGY 2 cut(s) 94, 952
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 752
Bse8I GATNNNNATC 1 cut(s) 818
BseDI CCNNGG 2 cut(s) 89, 354
BseGI GGATG 2 cut(s) 570, 824
BseJI GATNNNNATC 1 cut(s) 818
BseLI CCNNNNNNNGG 2 cut(s) 90, 109
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 4 cut(s) 351, 455, 714, 872
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 20, 441
BseX3I CGGCCG 2 cut(s) 92, 111
BseXI GCAGC 2 cut(s) 497, 731
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 2 cut(s) 95, 114
BshFI GGCC 3 cut(s) 94, 113, 267
BsiEI CGRYCG 2 cut(s) 95, 114
BsiSI CCGG 2 cut(s) 95, 953
BslI CCNNNNNNNGG 2 cut(s) 90, 109
BsmAI GTCTC 1 cut(s) 676
BsnI GGCC 3 cut(s) 94, 113, 267
Bsp143I GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
Bsp1720I GCTNAGC 1 cut(s) 870
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 7 cut(s) 89, 91, 114, 254, 318, 731, 899
BspANI GGCC 3 cut(s) 94, 113, 267
BspCNI CTCAG 4 cut(s) 352, 454, 715, 873
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 203
BspMAI CTGCAG 2 cut(s) 515, 833
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 2 cut(s) 80, 224
BsrDI GCAATG 1 cut(s) 752
BsrFI RCCGGY 2 cut(s) 94, 952
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 2 cut(s) 94, 952
BssECI CCNNGG 2 cut(s) 89, 354
BssMI GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 1 cut(s) 354
Bst2BI CACGAG 1 cut(s) 277
Bst4CI ACNGT 3 cut(s) 147, 961, 1051
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 5 cut(s) 96, 100, 152, 265, 954
BstDEI CTNAG 5 cut(s) 360, 441, 723, 870, 881
BstDSI CCRYGG 2 cut(s) 89, 354
BstF5I GGATG 2 cut(s) 570, 824
BstFNI CGCG 1 cut(s) 91
BstKTI GATC 8 cut(s) 81, 88, 219, 497, 532, 708, 816, 1024
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
BstMCI CGRYCG 2 cut(s) 95, 114
BstMWI GCNNNNNNNGC 2 cut(s) 273, 728
BstNSI RCATGY 1 cut(s) 635
BstPAI GACNNNNGTC 1 cut(s) 597
BstSFI CTRYAG 2 cut(s) 511, 829
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 2 cut(s) 497, 731
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BstZI CGGCCG 2 cut(s) 92, 111
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 3 cut(s) 94, 113, 267
BtgI CCRYGG 2 cut(s) 89, 354
BtsCI GGATG 2 cut(s) 570, 824
BtsI GCAGTG 3 cut(s) 520, 838, 1011
BtsIMutI CAGTG 7 cut(s) 152, 488, 504, 520, 838, 1011, 1044
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 5 cut(s) 96, 100, 152, 265, 954
CaiI CAGNNNCTG 1 cut(s) 151
Cfr10I RCCGGY 2 cut(s) 94, 952
Cfr42I CCGCGG 1 cut(s) 92
Csp6I GTAC 2 cut(s) 342, 445
CspCI CAANNNNNGTGG 2 cut(s) 673, 708
CviAII CATG 4 cut(s) 25, 206, 355, 632
CviQI GTAC 2 cut(s) 342, 445
DdeI CTNAG 5 cut(s) 360, 441, 723, 870, 881
DpnI GATC 8 cut(s) 80, 87, 218, 496, 531, 707, 815, 1023
DpnII GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
EaeI YGGCCR 3 cut(s) 92, 111, 265
EagI CGGCCG 2 cut(s) 92, 111
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 746
EclXI CGGCCG 2 cut(s) 92, 111
Eco130I CCWWGG 1 cut(s) 354
Eco52I CGGCCG 2 cut(s) 92, 111
Eco57I CTGAAG 2 cut(s) 915, 1020
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 4 cut(s) 28, 209, 358, 635
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 4 cut(s) 24, 205, 354, 631
FbaI TGATCA 1 cut(s) 1021
FblI GTMKAC 1 cut(s) 601
Fnu4HI GCNGC 5 cut(s) 92, 114, 319, 511, 720
FokI GGATG 2 cut(s) 557, 811
Fsp4HI GCNGC 5 cut(s) 92, 114, 319, 511, 720
FspBI CTAG 1 cut(s) 524
GluI GCNGC 5 cut(s) 92, 114, 319, 511, 720
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 3 cut(s) 94, 113, 267
HapII CCGG 2 cut(s) 95, 953
Hin1II CATG 4 cut(s) 28, 209, 358, 635
HinfI GANTC 7 cut(s) 50, 191, 224, 327, 598, 734, 978
HpaII CCGG 2 cut(s) 95, 953
HphI GGTGA 3 cut(s) 13, 73, 481
Hpy166II GTNNAC 3 cut(s) 272, 602, 992
Hpy188I TCNGA 4 cut(s) 348, 592, 681, 739
Hpy188III TCNNGA 4 cut(s) 76, 556, 938, 1025
Hpy8I GTNNAC 3 cut(s) 272, 602, 992
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 82, 721, 935
HpyCH4III ACNGT 3 cut(s) 147, 961, 1051
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 7 cut(s) 154, 513, 657, 719, 757, 831, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 728
HpyF3I CTNAG 5 cut(s) 360, 441, 723, 870, 881
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 4 cut(s) 28, 209, 358, 635
KroI GCCGGC 2 cut(s) 94, 952
KroNI GCCGGC 2 cut(s) 96, 954
Ksp22I TGATCA 1 cut(s) 1021
KspI CCGCGG 1 cut(s) 92
Kzo9I GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
LmnI GCTCC 3 cut(s) 33, 207, 247
Lsp1109I GCAGC 2 cut(s) 497, 731
MaeI CTAG 1 cut(s) 524
MaeII ACGT 1 cut(s) 196
MaeIII GTNAC 3 cut(s) 19, 469, 1045
MalI GATC 8 cut(s) 80, 87, 218, 496, 531, 707, 815, 1023
MboI GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
MboII GAAGA 6 cut(s) 197, 410, 512, 700, 782, 823
MflI RGATCY 1 cut(s) 529
MluCI AATT 6 cut(s) 504, 547, 800, 850, 888, 1057
MlyI GAGTC 4 cut(s) 185, 321, 607, 728
MmeI TCCRAC 3 cut(s) 166, 194, 276
MnlI CCTC 9 cut(s) 41, 181, 228, 382, 450, 462, 482, 919, 1021
MroNI GCCGGC 2 cut(s) 94, 952
MroXI GAANNNNTTC 1 cut(s) 549
MspA1I CMGCKG 4 cut(s) 91, 318, 722, 1019
MspI CCGG 2 cut(s) 95, 953
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 2 cut(s) 273, 728
NaeI GCCGGC 2 cut(s) 96, 954
NcoI CCATGG 1 cut(s) 354
NdeII GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
NgoMIV GCCGGC 2 cut(s) 94, 952
NlaIII CATG 4 cut(s) 28, 209, 358, 635
NlaIV GGNNCC 1 cut(s) 203
NmuCI GTSAC 3 cut(s) 19, 469, 1045
NspI RCATGY 1 cut(s) 635
PciI ACATGT 1 cut(s) 631
PdiI GCCGGC 2 cut(s) 96, 954
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 3 cut(s) 50, 224, 978
PflFI GACNNNGTC 2 cut(s) 7, 122
PkrI GCNGC 5 cut(s) 93, 115, 320, 512, 721
PleI GAGTC 4 cut(s) 185, 321, 606, 728
PpsI GAGTC 4 cut(s) 185, 321, 606, 728
PscI ACATGT 1 cut(s) 631
PshAI GACNNNNGTC 1 cut(s) 597
PsiI TTATAA 1 cut(s) 621
PspN4I GGNNCC 1 cut(s) 203
PstI CTGCAG 2 cut(s) 515, 833
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 529
PsyI GACNNNGTC 2 cut(s) 7, 122
PvuII CAGCTG 2 cut(s) 722, 1019
RsaI GTAC 2 cut(s) 343, 446
RsaNI GTAC 2 cut(s) 342, 445
SacII CCGCGG 1 cut(s) 92
SatI GCNGC 5 cut(s) 92, 114, 319, 511, 720
Sau3AI GATC 8 cut(s) 78, 85, 216, 494, 529, 705, 813, 1021
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 4 cut(s) 185, 321, 607, 728
SetI ASST 8 cut(s) 199, 471, 512, 607, 724, 846, 930, 1021
SfcI CTRYAG 2 cut(s) 511, 829
Sfr303I CCGCGG 1 cut(s) 92
SgrBI CCGCGG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 6 cut(s) 504, 547, 800, 850, 888, 1057
SsiI CCGC 7 cut(s) 89, 91, 114, 254, 318, 731, 899
SspI AATATT 1 cut(s) 138
SspMI CTAG 1 cut(s) 524
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 147, 961, 1051
TaiI ACGT 1 cut(s) 199
TaqI TCGA 3 cut(s) 233, 333, 930
TasI AATT 6 cut(s) 504, 547, 800, 850, 888, 1057
TatI WGTACW 1 cut(s) 444
TauI GCSGC 3 cut(s) 94, 116, 321
TfiI GAWTC 3 cut(s) 50, 224, 978
TscAI CASTG 7 cut(s) 152, 488, 504, 520, 838, 1011, 1044
TseFI GTSAC 3 cut(s) 19, 469, 1045
TseI GCWGC 2 cut(s) 510, 719
Tsp45I GTSAC 3 cut(s) 19, 469, 1045
TspDTI ATGAA 3 cut(s) 444, 762, 970
TspGWI ACGGA 2 cut(s) 43, 401
TspRI CASTG 7 cut(s) 152, 488, 504, 520, 838, 1011, 1044
Tth111I GACNNNGTC 2 cut(s) 7, 122
XapI RAATTY 2 cut(s) 547, 850
XceI RCATGY 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 1 cut(s) 524
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.