Rw7G018870

taxadien-5-alpha-ol O-acetyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014513
Physical Location & Seq
Forward (+)
20592408 .. 20593543
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018870.1

Sequence Viewer

Length: 1056 bp
ATGACATTGTCCAATGGTGTCACCATGCTCCTCCCAGAAGCACACATTGAATCCGTCCAGTCTGTAATGCCCTTCAAGATCACCGATCCGCGCCCAGCACGCCAAGTTTCGGCCGCAGGCCCCGTCGGTTTGGGAATATTCCAACAGTGCCTGCACATAGTTCTCTACTACACAAAAGCAACCGAAGAGGACTCACGTTGGCTCCATGCTGGTTGGATCAAGGAATCACTTTCGAGGGCGTTGCTGGAGCAACCACTATTTGCTGGCCGTTTACGCACGAGTGATGGGGGAGAGTTGGAGATTGTGTCAAATGACAGCGGCGTTAGACTCGTCGAAACTCGTACTCCGATGACCATGGCTGAGTTTCTGTCTTACAAGGAGGATACAGAAGATAAACTTGTGTTTTGGAAAACTATTGATGAACGAAGTCCTCAGTACTCTCCTCTCTTTTATGTTCAGGTGACATATTTTCAGTGTGGAGGGAGATCAGTGGGAATTAGCTGCAGTGTTCTTCTAGCAGATCTTTTGTTCAAAGAAAATTTTCTCAAAAAATGGGCTAACATCCACCACAATCTGCTTTCAGACGAGAGTCTACCTGCGACACCCTTTTATAACTTCCCACATGTTGAAAAAAGTGGAAATCCCTGCAAGAATATATTTGTCTCTGTTCCGAGCAAAAAATGTGGCAAGACAATGATCTTCAATGTTGCAGCTGAGAAGGCGGACTCGGAGAACGATGATGCATTGCAACTTTGTGTTGAAGAAGCAGAGAGCAAGTTTGGTTTTGGGAAATTGTTTGTGAAGATCAACATCTCATCCTGCAGTGCAATCAAGGTTGAGAATTTTCAAAAAGAAGGACTTGCTAAGCCGAAACTGAGCCTAATTGCTGAAGCGGATTGGGATTATTTCGGGGCAAAGGAGGTCGAGTTTCGTGAAGGGAATAAGCCGGCTACTGTTTCGTATTGGTTTGGATTCATTTCTGGTGGACTTGTTATGGCAGTGCCATCATCAGCTGATCAGGAGGGTGCTTCAGTGATTGTCACAGTTCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

351

Amino Acids

38.93

Weight (kDa)

5.42

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 54 - 191 9.7e-23 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02890
fragaria_vesca FvH4_5g20090
malus_domestica MD04G1050800.v1.1
prunus_persica Prupe.5G059300_v2.0.a1
pyrus_communis pycom04g04470
rosa_chinensis RchiOBHm_Chr7g0204981
rosa_laevigata RLG00000003418
rosa_multiflora Rmu_sc0005112.1_g000025 Rmu_sc0024226.1_g000001
rosa_roxburghii Rroxscaffold_3G00252630
rosa_rugosa Rorug07G0086800
rosa_samantha Rh7AG217800 Rh7BG214700 Rh7CG230800 Rh7CG230900 Rh7DG225100
rosa_wichuraiana Rw7G018870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 592
AccII CGCG 1 cut(s) 91
AciI CCGC 5 cut(s) 89, 114, 318, 722, 893
AclWI GGATC 2 cut(s) 80, 224
AcoI YGGCCR 2 cut(s) 111, 265
AcsI RAATTY 2 cut(s) 538, 841
AcuI CTGAAG 2 cut(s) 909, 1014
AfaI GTAC 2 cut(s) 343, 437
AfiI CCNNNNNNNGG 1 cut(s) 109
AflIII ACRYGT 1 cut(s) 622
AgsI TTSAA 7 cut(s) 50, 76, 532, 629, 703, 761, 848
AluBI AGCT 3 cut(s) 501, 713, 1013
AluI AGCT 3 cut(s) 501, 713, 1013
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 2 cut(s) 80, 224
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 3 cut(s) 111, 118, 265
ApeKI GCWGC 2 cut(s) 501, 710
ApoI RAATTY 2 cut(s) 538, 841
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 13, 73, 472
BauI CACGAG 1 cut(s) 277
BbvI GCAGC 2 cut(s) 488, 722
BccI CCATC 2 cut(s) 278, 1012
BceAI ACGGC 1 cut(s) 252
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 1 cut(s) 1015
BcoDI GTCTC 1 cut(s) 667
BfaI CTAG 1 cut(s) 515
BfmI CTRYAG 2 cut(s) 502, 820
BfuAI ACCTGC 1 cut(s) 604
BfuI GTATCC 1 cut(s) 376
BglII AGATCT 1 cut(s) 520
BisI GCNGC 4 cut(s) 114, 319, 502, 711
BlpI GCTNAGC 1 cut(s) 864
BlsI GCNGC 4 cut(s) 115, 320, 503, 712
BmcAI AGTACT 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 121, 203
BmsI GCATC 1 cut(s) 730
BoxI GACNNNNGTC 1 cut(s) 588
BpmI CTGGAG 1 cut(s) 266
Bpu1102I GCTNAGC 1 cut(s) 864
BsaBI GATNNNNATC 1 cut(s) 809
BsaJI CCNNGG 1 cut(s) 354
BsaXI ACNNNNNCTCC 8 cut(s) 186, 216, 290, 320, 328, 358, 911, 941
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 1 cut(s) 946
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 743
Bse8I GATNNNNATC 1 cut(s) 809
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 2 cut(s) 561, 815
BseJI GATNNNNATC 1 cut(s) 809
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMI GCAATG 1 cut(s) 743
BseMII CTCAG 4 cut(s) 351, 446, 705, 866
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 20, 432
BseX3I CGGCCG 1 cut(s) 111
BseXI GCAGC 2 cut(s) 488, 722
BseYI CCCAGC 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 113, 120, 267
BsiEI CGRYCG 1 cut(s) 114
BsiSI CCGG 1 cut(s) 947
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 667
BsnI GGCC 3 cut(s) 113, 120, 267
Bsp143I GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
Bsp1720I GCTNAGC 1 cut(s) 864
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 5 cut(s) 89, 114, 318, 722, 893
BspANI GGCC 3 cut(s) 113, 120, 267
BspCNI CTCAG 4 cut(s) 352, 445, 706, 867
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 121, 203
BspMAI CTGCAG 2 cut(s) 506, 824
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 2 cut(s) 80, 224
BsrDI GCAATG 1 cut(s) 743
BsrFI RCCGGY 1 cut(s) 946
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 946
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 1 cut(s) 354
Bst2BI CACGAG 1 cut(s) 277
Bst4CI ACNGT 3 cut(s) 147, 955, 1045
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 5 cut(s) 100, 118, 152, 265, 948
BstDEI CTNAG 5 cut(s) 360, 432, 714, 864, 875
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 561, 815
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 8 cut(s) 81, 88, 219, 488, 523, 699, 807, 1018
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 3 cut(s) 99, 273, 719
BstNSI RCATGY 1 cut(s) 626
BstPAI GACNNNNGTC 1 cut(s) 588
BstSFI CTRYAG 2 cut(s) 502, 820
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 2 cut(s) 488, 722
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BstZI CGGCCG 1 cut(s) 111
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 3 cut(s) 113, 120, 267
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 2 cut(s) 561, 815
BtsI GCAGTG 3 cut(s) 511, 829, 1005
BtsIMutI CAGTG 7 cut(s) 152, 479, 495, 511, 829, 1005, 1038
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 5 cut(s) 100, 118, 152, 265, 948
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 946
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 342, 436
CspCI CAANNNNNGTGG 2 cut(s) 664, 699
CviAII CATG 4 cut(s) 25, 206, 355, 623
CviQI GTAC 2 cut(s) 342, 436
DdeI CTNAG 5 cut(s) 360, 432, 714, 864, 875
DpnI GATC 8 cut(s) 80, 87, 218, 487, 522, 698, 806, 1017
DpnII GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
EaeI YGGCCR 2 cut(s) 111, 265
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 737
EclXI CGGCCG 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 354
Eco52I CGGCCG 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 909, 1014
EcoO109I RGGNCCY 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 745
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 4 cut(s) 28, 209, 358, 626
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 843, 875
FatI CATG 4 cut(s) 24, 205, 354, 622
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 1 cut(s) 592
Fnu4HI GCNGC 4 cut(s) 114, 319, 502, 711
FokI GGATG 2 cut(s) 548, 802
Fsp4HI GCNGC 4 cut(s) 114, 319, 502, 711
FspBI CTAG 1 cut(s) 515
GlaI GCGC 1 cut(s) 92
GluI GCNGC 4 cut(s) 114, 319, 502, 711
GsaI CCCAGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 3 cut(s) 113, 120, 267
HapII CCGG 1 cut(s) 947
HhaI GCGC 1 cut(s) 93
Hin1II CATG 4 cut(s) 28, 209, 358, 626
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 7 cut(s) 50, 191, 224, 327, 589, 725, 972
HpaII CCGG 1 cut(s) 947
HphI GGTGA 3 cut(s) 13, 73, 472
Hpy166II GTNNAC 3 cut(s) 272, 593, 986
Hpy188I TCNGA 4 cut(s) 348, 583, 672, 730
Hpy188III TCNNGA 3 cut(s) 76, 932, 1019
Hpy8I GTNNAC 3 cut(s) 272, 593, 986
Hpy99I CGWCG 2 cut(s) 128, 335
HpyAV CCTTC 4 cut(s) 82, 712, 848, 929
HpyCH4III ACNGT 3 cut(s) 147, 955, 1045
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 8 cut(s) 154, 504, 648, 710, 743, 748, 822, 827
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 273, 719
HpyF3I CTNAG 5 cut(s) 360, 432, 714, 864, 875
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 4 cut(s) 28, 209, 358, 626
HspAI GCGC 1 cut(s) 91
KroI GCCGGC 1 cut(s) 946
KroNI GCCGGC 1 cut(s) 948
Ksp22I TGATCA 1 cut(s) 1015
Kzo9I GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
LmnI GCTCC 3 cut(s) 33, 207, 247
Lsp1109I GCAGC 2 cut(s) 488, 722
LweI GCATC 1 cut(s) 730
MaeI CTAG 1 cut(s) 515
MaeII ACGT 1 cut(s) 196
MaeIII GTNAC 3 cut(s) 19, 460, 1039
MalI GATC 8 cut(s) 80, 87, 218, 487, 522, 698, 806, 1017
MboI GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
MboII GAAGA 6 cut(s) 197, 401, 503, 691, 773, 814
MflI RGATCY 1 cut(s) 520
MluCI AATT 6 cut(s) 495, 538, 791, 841, 882, 1051
MlyI GAGTC 4 cut(s) 185, 321, 598, 719
MmeI TCCRAC 3 cut(s) 166, 194, 276
MnlI CCTC 9 cut(s) 41, 181, 228, 373, 441, 453, 473, 913, 1015
Mph1103I ATGCAT 1 cut(s) 745
MroNI GCCGGC 1 cut(s) 946
MroXI GAANNNNTTC 1 cut(s) 540
MspA1I CMGCKG 3 cut(s) 318, 713, 1013
MspI CCGG 1 cut(s) 947
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 3 cut(s) 99, 273, 719
NaeI GCCGGC 1 cut(s) 948
NcoI CCATGG 1 cut(s) 354
NdeII GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
NgoMIV GCCGGC 1 cut(s) 946
NlaIII CATG 4 cut(s) 28, 209, 358, 626
NlaIV GGNNCC 2 cut(s) 121, 203
NmuCI GTSAC 3 cut(s) 19, 460, 1039
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 1 cut(s) 626
PciI ACATGT 1 cut(s) 622
PdiI GCCGGC 1 cut(s) 948
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 50, 224, 972
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 115, 320, 503, 712
PleI GAGTC 4 cut(s) 185, 321, 597, 719
PpsI GAGTC 4 cut(s) 185, 321, 597, 719
PscI ACATGT 1 cut(s) 622
PshAI GACNNNNGTC 1 cut(s) 588
PsiI TTATAA 1 cut(s) 612
PspFI CCCAGC 1 cut(s) 94
PspN4I GGNNCC 2 cut(s) 121, 203
PspPI GGNCC 1 cut(s) 119
PstI CTGCAG 2 cut(s) 506, 824
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 2 cut(s) 713, 1013
RsaI GTAC 2 cut(s) 343, 437
RsaNI GTAC 2 cut(s) 342, 436
SatI GCNGC 4 cut(s) 114, 319, 502, 711
Sau3AI GATC 8 cut(s) 78, 85, 216, 485, 520, 696, 804, 1015
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 1 cut(s) 437
SchI GAGTC 4 cut(s) 185, 321, 598, 719
SetI ASST 8 cut(s) 199, 462, 503, 598, 715, 837, 924, 1015
SfaNI GCATC 1 cut(s) 730
SfcI CTRYAG 2 cut(s) 502, 820
Sse9I AATT 6 cut(s) 495, 538, 791, 841, 882, 1051
SsiI CCGC 5 cut(s) 89, 114, 318, 722, 893
SspI AATATT 1 cut(s) 138
SspMI CTAG 1 cut(s) 515
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 147, 955, 1045
TaiI ACGT 1 cut(s) 199
TaqI TCGA 3 cut(s) 233, 333, 924
TasI AATT 6 cut(s) 495, 538, 791, 841, 882, 1051
TatI WGTACW 1 cut(s) 435
TauI GCSGC 2 cut(s) 116, 321
TfiI GAWTC 3 cut(s) 50, 224, 972
TscAI CASTG 7 cut(s) 152, 479, 495, 511, 829, 1005, 1038
TseFI GTSAC 3 cut(s) 19, 460, 1039
TseI GCWGC 2 cut(s) 501, 710
Tsp45I GTSAC 3 cut(s) 19, 460, 1039
TspDTI ATGAA 2 cut(s) 435, 964
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 7 cut(s) 152, 479, 495, 511, 829, 1005, 1038
Tth111I GACNNNGTC 1 cut(s) 7
XapI RAATTY 2 cut(s) 538, 841
XceI RCATGY 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 1 cut(s) 515
ZrmI AGTACT 1 cut(s) 437
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.