MD04G1052200.v1.1

Belongs to the bacterial ribosomal protein bL35 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013010
Physical Location & Seq
Reverse (-)
6122910 .. 6124978
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1052200.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCTTGTGTAGGTGTAACCATGACCATGGCGGCATCGTTTGGTCTGAGATACTACCCATCGTGCTCTTACCCAACTCAACTCAATCACGGCTCGGTTCAACTCGGTAGGTTCAAGAGGCCCAAACCCTTGAGACTCAGTTCCTCGCAATCGCATAGCATTTCTGGGTTGGACTCGCTTCTTCCCCGCAAGCTCTGCGCTATCAATTTCACCCCTCCTCGCCGCCTTAGCTCTCTTACCATCGTCTCCGCCAAAGGCTACAAAATGAAGACCCACAAGGCTTCGGCGAAGCGCTTCAGGGTGACGGGTAGAGGAAAAATAGTTCGGAGGAGAGCTGGAAAGCAACATTTACTTGTAAAGAAGAATGCCAAGAGGAGAACTCGACTCTCCAAAATGCATGCAGTTAGTCGAAGTGACTATGACAACGTGATTGGAGCCTTGCCATATCTGAAAGTAAATAGGAAGGCAGAGTAA

Protein Analysis

158

Amino Acids

17.6

Weight (kDa)

11.54

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 87 - 143 1.1e-19 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24090
fragaria_vesca FvH4_4g09690 FvH4_4g09690
malus_domestica MD04G1052200.v1.1
prunus_persica Prupe.1G102100_v2.0.a1
pyrus_communis pycom04g04590
rosa_chinensis RchiOBHm_Chr4g0405501
rosa_laevigata RLG00000008838
rosa_multiflora Rmu_sc0003981.1_g000024 Rmu_sc0005023.1_g000021
rosa_roxburghii Rroxscaffold_5G00350000
rosa_rugosa Rorug04G0060600
rosa_samantha Rh4AG132300 Rh4BG127200 Rh4CG139800 Rh4DG126600
rosa_wichuraiana Rw0G018140 Rw4G010710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 32, 187, 223, 249
AcuI CTGAAG 1 cut(s) 280
AfeI AGCGCT 1 cut(s) 293
AgsI TTSAA 2 cut(s) 101, 115
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 3 cut(s) 193, 231, 335
AluI AGCT 3 cut(s) 193, 231, 335
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 2 cut(s) 127, 250
Aor51HI AGCGCT 1 cut(s) 293
AoxI GGCC 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 2 cut(s) 200, 294
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 202, 313
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 68
BccI CCATC 2 cut(s) 67, 248
BceAI ACGGC 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 127, 250
BfoI RGCGCY 1 cut(s) 295
BisI GCNGC 2 cut(s) 33, 223
BlsI GCNGC 2 cut(s) 34, 224
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 436
BmsI GCATC 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 364, 396
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 27
BseMII CTCAG 2 cut(s) 38, 151
BseRI GAGGAG 3 cut(s) 207, 343, 388
BshFI GGCC 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 68
BsmAI GTCTC 2 cut(s) 127, 250
BsmBI CGTCTC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 68
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 4 cut(s) 32, 187, 223, 249
BspANI GGCC 1 cut(s) 121
BspCNI CTCAG 2 cut(s) 39, 150
BspLI GGNNCC 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 27
BstAPI GCANNNNNTGC 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 191, 399
BstDEI CTNAG 3 cut(s) 47, 137, 227
BstDSI CCRYGG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 200, 294
BstMAI GTCTC 2 cut(s) 127, 250
BstMWI GCNNNNNNNGC 2 cut(s) 195, 228
BstNSI RCATGY 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 275
BstXI CCANNNNNNTGG 1 cut(s) 28
BsuRI GGCC 1 cut(s) 121
BtgI CCRYGG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 191, 399
CfoI GCGC 2 cut(s) 200, 294
Cfr13I GGNCC 1 cut(s) 120
CviAII CATG 3 cut(s) 22, 28, 398
CviJI RGCY 9 cut(s) 5, 93, 121, 193, 231, 258, 281, 335, 437
CviKI_1 RGCY 9 cut(s) 5, 93, 121, 193, 231, 258, 281, 335, 437
DdeI CTNAG 3 cut(s) 47, 137, 227
EciI GGCGGA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 27
Eco47III AGCGCT 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 250
FaeI CATG 3 cut(s) 25, 31, 401
FaiI YATR 6 cut(s) 23, 29, 156, 399, 420, 445
FatI CATG 3 cut(s) 21, 27, 397
FauI CCCGC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 33, 223
Fsp4HI GCNGC 2 cut(s) 33, 223
GlaI GCGC 2 cut(s) 199, 293
GluI GCNGC 2 cut(s) 33, 223
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 1 cut(s) 121
HhaI GCGC 2 cut(s) 200, 294
Hin1II CATG 3 cut(s) 25, 31, 401
Hin6I GCGC 2 cut(s) 198, 292
HinP1I GCGC 2 cut(s) 198, 292
HinfI GANTC 3 cut(s) 135, 173, 384
HphI GGTGA 2 cut(s) 202, 313
Hpy188I TCNGA 3 cut(s) 48, 327, 450
Hpy188III TCNNGA 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 457
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 2 cut(s) 397, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 228
HpyF3I CTNAG 3 cut(s) 47, 137, 227
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 3 cut(s) 25, 31, 401
HspAI GCGC 2 cut(s) 198, 292
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 3 cut(s) 150, 283, 321
LweI GCATC 1 cut(s) 44
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 3 cut(s) 16, 301, 413
MboII GAAGA 3 cut(s) 173, 280, 373
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 1 cut(s) 205
MlyI GAGTC 3 cut(s) 129, 167, 378
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 7 cut(s) 111, 154, 225, 228, 305, 321, 366
Mph1103I ATGCAT 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 370
MwoI GCNNNNNNNGC 2 cut(s) 195, 228
NcoI CCATGG 1 cut(s) 27
NlaIII CATG 3 cut(s) 25, 31, 401
NlaIV GGNNCC 1 cut(s) 436
NmuCI GTSAC 2 cut(s) 301, 413
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 1 cut(s) 401
PaeI GCATGC 1 cut(s) 401
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 293
PkrI GCNGC 2 cut(s) 34, 224
PleI GAGTC 3 cut(s) 129, 167, 378
PpsI GAGTC 3 cut(s) 129, 167, 378
PspN4I GGNNCC 1 cut(s) 436
PspPI GGNCC 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 26
SatI GCNGC 2 cut(s) 33, 223
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 3 cut(s) 129, 167, 378
SduI GDGCHC 1 cut(s) 68
SetI ASST 6 cut(s) 16, 113, 195, 233, 337, 429
SfaNI GCATC 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 130
SmoI CTYRAG 1 cut(s) 130
SphI GCATGC 1 cut(s) 401
Sse9I AATT 1 cut(s) 205
SsiI CCGC 4 cut(s) 32, 187, 223, 249
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 429
TaqI TCGA 2 cut(s) 382, 409
TasI AATT 1 cut(s) 205
TauI GCSGC 2 cut(s) 35, 225
TseFI GTSAC 2 cut(s) 301, 413
Tsp45I GTSAC 2 cut(s) 301, 413
TspDTI ATGAA 1 cut(s) 281
XceI RCATGY 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.