Rh4BG127200

Belongs to the bacterial ribosomal protein bL35 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013010
Physical Location & Seq
Forward (+)
21253876 .. 21256354
2479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG127200.1

Sequence Viewer

Length: 414 bp
ATGGCTTCCGCGACTGTGACCATGACGGTGCCGTTTGGTCTGAGATATTCCCCATTATGCTCTTGCCGGGTGGGTCAAGCCCCGGTTCAGCTCCCTCAACTCAACCGGCCCAACTCGTTGAGACTCAGTTCCTCACACACCATTTCTGGGTTCGGCTCGCTTCTTCCCCTCAGGCTTTGCACTATCACTTCCACACCTCTTAGGCTTCGCTCCCTCACCATTGTGGCACATAAAGGCTACAAAATGAAGACCCACAAGGCTTCGTCAAAGCGGTTCAGGGTGACAGGTAGAGGGAAAATAGTTCGGAGGAGAGCTCGAAAGCAACATTTACTTGTGAAGAAGAATGCCAAGAGGAGAAACCGGCTCTCCAAAATGGTGTGTTTCTTAAACATTGTTCCAAGTTTAGTTATATAA

Protein Analysis

137

Amino Acids

15.36

Weight (kDa)

12.0

Isoelectric Point (pI)

69.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 80 - 126 4.9e-16 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24090
fragaria_vesca FvH4_4g09690 FvH4_4g09690
malus_domestica MD04G1052200.v1.1
prunus_persica Prupe.1G102100_v2.0.a1
pyrus_communis pycom04g04590
rosa_chinensis RchiOBHm_Chr4g0405501
rosa_laevigata RLG00000008838
rosa_multiflora Rmu_sc0003981.1_g000024 Rmu_sc0005023.1_g000021
rosa_roxburghii Rroxscaffold_5G00350000
rosa_rugosa Rorug04G0060600
rosa_samantha Rh4AG132300 Rh4BG127200 Rh4CG139800 Rh4DG126600
rosa_wichuraiana Rw0G018140 Rw4G010710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 28
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 9, 271
AfiI CCNNNNNNNGG 1 cut(s) 147
AleI CACNNNNGTG 1 cut(s) 221
AluBI AGCT 2 cut(s) 91, 314
AluI AGCT 2 cut(s) 91, 314
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 115
AoxI GGCC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 2 cut(s) 68, 83
AsuHPI GGTGA 2 cut(s) 208, 292
AxyI CCTNAGG 1 cut(s) 170
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 316
BceAI ACGGC 1 cut(s) 16
BcnI CCSGG 2 cut(s) 68, 83
BcoDI GTCTC 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 68, 83
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 68, 83
BpiI GAAGAC 1 cut(s) 254
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpuMI CCSGG 2 cut(s) 68, 83
BsaJI CCNNGG 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse118I RCCGGY 2 cut(s) 105, 360
Bse21I CCTNAGG 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 3 cut(s) 32, 139, 184
BseRI GAGGAG 2 cut(s) 322, 367
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 109
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 4 cut(s) 67, 83, 106, 361
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 115
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 316
BspACI CCGC 2 cut(s) 9, 271
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 3 cut(s) 33, 138, 183
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 28
BsrFI RCCGGY 2 cut(s) 105, 360
BssAI RCCGGY 2 cut(s) 105, 360
BssECI CCNNGG 1 cut(s) 81
Bst4CI ACNGT 2 cut(s) 16, 28
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 4 cut(s) 41, 125, 170, 200
BstFNI CGCG 1 cut(s) 11
BstMAI GTCTC 1 cut(s) 115
BstSCI CCNGG 2 cut(s) 66, 81
BstUI CGCG 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 254
Bsu36I CCTNAGG 1 cut(s) 170
BsuRI GGCC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 158
Cfr10I RCCGGY 2 cut(s) 105, 360
Cfr13I GGNCC 1 cut(s) 108
CviAII CATG 1 cut(s) 22
DdeI CTNAG 4 cut(s) 41, 125, 170, 200
Ecl136II GAGCTC 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 314
Eco81I CCTNAGG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 316
FaeI CATG 1 cut(s) 25
FaiI YATR 5 cut(s) 23, 58, 231, 410, 412
FatI CATG 1 cut(s) 21
FriOI GRGCYC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 109
HapII CCGG 4 cut(s) 67, 83, 106, 361
Hin1II CATG 1 cut(s) 25
HinfI GANTC 1 cut(s) 123
HpaII CCGG 4 cut(s) 67, 83, 106, 361
HphI GGTGA 2 cut(s) 208, 292
Hpy188I TCNGA 2 cut(s) 42, 306
HpyCH4III ACNGT 2 cut(s) 16, 28
HpyCH4V TGCA 1 cut(s) 180
HpyF3I CTNAG 4 cut(s) 41, 125, 170, 200
Hsp92II CATG 1 cut(s) 25
LmnI GCTCC 2 cut(s) 96, 215
LpnPI CCDG 8 cut(s) 80, 96, 119, 132, 157, 262, 270, 374
MaeIII GTNAC 2 cut(s) 16, 280
MboII GAAGA 4 cut(s) 155, 259, 349, 352
MhlI GDGCHC 1 cut(s) 316
MlyI GAGTC 1 cut(s) 117
MnlI CCTC 8 cut(s) 105, 142, 179, 207, 224, 284, 300, 345
MseI TTAA 1 cut(s) 386
MslI CAYNNNNRTG 2 cut(s) 26, 221
MspI CCGG 4 cut(s) 67, 83, 106, 361
MspR9I CCNGG 2 cut(s) 68, 83
Mva1269I GAATGC 1 cut(s) 349
MvnI CGCG 1 cut(s) 11
NciI CCSGG 2 cut(s) 68, 83
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 16, 280
OliI CACNNNNGTG 1 cut(s) 221
PctI GAATGC 1 cut(s) 349
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 108
RseI CAYNNNNRTG 2 cut(s) 26, 221
SacI GAGCTC 1 cut(s) 316
SaqAI TTAA 1 cut(s) 386
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 1 cut(s) 117
ScrFI CCNGG 2 cut(s) 68, 83
SduI GDGCHC 1 cut(s) 316
SetI ASST 4 cut(s) 93, 199, 289, 316
SmiMI CAYNNNNRTG 2 cut(s) 26, 221
SsiI CCGC 2 cut(s) 9, 271
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 2 cut(s) 66, 81
TaaI ACNGT 2 cut(s) 16, 28
TaqI TCGA 1 cut(s) 316
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TseFI GTSAC 2 cut(s) 16, 280
Tsp45I GTSAC 2 cut(s) 16, 280
TspDTI ATGAA 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.