Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006450

Physical Location & Seq

Genomic Coordinates Forward (+)

8771410 .. 8774548

Gene Structure 3139 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1065500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGCTGATCCAGAACGAGGACCGCCAACAAAACCCAGACCCACCAATTACAATTACAACTCCAACCAGTACTACGTGGGAACATCCGAAACCCAATGGACATCGTGGCTGATTCCTATGTTCGTGGTGGCCAATGTTGTTGTCTTCATCGTCGCCATGTCCATCAACAACTGCCCAAAACACAATCTTGGTTTCGCCGACAAGTGCGTGGCCAAGTTTCTGGGTCGGTTTTCGTTCGAGCCTCTCAGGGAGAACCCTCTGTTCGGTCCTTCTTCTGATACATTGGAAAAATTGGGAGCTCTTGAATGGTTCAAGGTTGTACATGGGCATCAAGGATGGAGGCTTTTCACTTGTATGTGGTTGCATGCTGGTGTCATCCATTTGCTTGCAAACATGCTCAGCTTGGTCTTTATTGGCATCCGTCTTGAGCAGCAGTTTGGGTTTGTACGCGTGGGGATAATCTACCTGTTGTCTGGATTTGGTGGGAGCATATTATCTGCCCTTTTCATTCAGAACAATATTTCTGTTGGTGCTTCTGGCGCTTTGTTTGGACTTCTCGGAGCTATGTTATCAGAGCTTATTACTAACTGGAGTATATACACAAATAAGGCCGCCGCACTATTGACGCTTATAGTCATTATCGCCATTAACTTAGCTGTTGGGATTCTTCCTCATGTTGACAACTTCGCCCACATTGGAGGATTCTTGAGTGGCTTTCTCCTTGGCTTTATTTTTCTACTTCGTCCCCAGTTTGGATGGACAGAGTCTCGGAATCTTGCAGCCAATGCTCGTGTCAAATCTAAGCATAAGCCTTACCAATATGTTCTTGTGTTGGTTGCCTCTGCCTTGCTGATTGCCGGATTAACTACAGGATTGGTGTTACTGTTCAAAGGAGAGAACTTAAACAAGCACTGCAGTTGGTGCCATTACCTAAGCTGCGTGCCAACGTCAAAATGGAAATGCGGGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0002791 GO:0002792 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005911 GO:0006508 GO:0006807 GO:0006928 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008283 GO:0009506 GO:0009894 GO:0009966 GO:0009987 GO:0010646 GO:0012505 GO:0016020 GO:0016477 GO:0016787 GO:0017171 GO:0019222 GO:0019538 GO:0023051 GO:0030054 GO:0030162 GO:0031090 GO:0031323 GO:0031329 GO:0031984 GO:0032268 GO:0032879 GO:0032880 GO:0040011 GO:0042058 GO:0042175 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048870 GO:0050708 GO:0050709 GO:0050789 GO:0050794 GO:0051046 GO:0051048 GO:0051049 GO:0051051 GO:0051171 GO:0051179 GO:0051223 GO:0051224 GO:0051246 GO:0051674 GO:0055044 GO:0060255 GO:0061136 GO:0065007 GO:0070011 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0098588 GO:0098791 GO:0098827 GO:0140096 GO:1901184 GO:1901564 GO:1903050 GO:1903362 GO:1903530 GO:1903531 GO:1904950

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhomboid

Protein Analysis

324

Amino Acids

35.71

Weight (kDa)

9.34

Isoelectric Point (pI)

22.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhomboid	PF01694	110 - 246	1.9e-43	Rhomboid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12750 AT1G12750 AT1G12750 AT1G12750 AT1G12750 AT1G12750 AT1G12750 AT1G63120 AT4G23070
fragaria_vesca	FvH4_5g01700 FvH4_5g01700 FvH4_5g01700
malus_domestica	MD04G1065500.v1.1
prunus_persica	Prupe.5G092700_v2.0.a1
pyrus_communis	pycom04g05900 pycom06g07360
rosa_chinensis	RchiOBHm_Chr7g0199021
rosa_laevigata	RLG00000003823
rosa_multiflora	Rmu_sc0006266.1_g000029
rosa_roxburghii	Rroxscaffold_3G00257210
rosa_rugosa	Rorug07G0052100
rosa_samantha	Rh7AG175200 Rh7BG178600 Rh7CG184400 Rh7DG178000
rosa_wichuraiana	Rw7G015370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	921
AccII	CGCG	1 cut(s)	450
AciI	CCGC	4 cut(s)	23, 612, 615, 963
AclWI	GGATC	1 cut(s)	2
AcoI	YGGCCR	2 cut(s)	129, 210
AfaI	GTAC	3 cut(s)	71, 321, 447
AfiI	CCNNNNNNNGG	3 cut(s)	17, 263, 752
AflIII	ACRYGT	1 cut(s)	448
AgsI	TTSAA	3 cut(s)	305, 313, 889
AluBI	AGCT	6 cut(s)	299, 402, 563, 577, 656, 936
AluI	AGCT	6 cut(s)	299, 402, 563, 577, 656, 936
Alw21I	GWGCWC	1 cut(s)	301
Alw26I	GTCTC	1 cut(s)	771
AlwI	GGATC	1 cut(s)	2
AoxI	GGCC	3 cut(s)	129, 210, 609
ApeKI	GCWGC	3 cut(s)	430, 779, 936
AspLEI	GCGC	1 cut(s)	542
AspS9I	GGNCC	2 cut(s)	20, 266
AvaII	GGWCC	2 cut(s)	20, 266
BalI	TGGCCA	2 cut(s)	131, 212
BanI	GGYRCC	1 cut(s)	921
BanII	GRGCYC	1 cut(s)	301
BauI	CACGAG	1 cut(s)	789
BbsI	GAAGAC	1 cut(s)	136
Bbv12I	GWGCWC	1 cut(s)	301
BbvI	GCAGC	3 cut(s)	442, 791, 923
BccI	CCATC	3 cut(s)	170, 330, 750
BcoDI	GTCTC	1 cut(s)	771
BfmI	CTRYAG	2 cut(s)	867, 913
BfoI	RGCGCY	1 cut(s)	543
BisI	GCNGC	5 cut(s)	431, 612, 615, 780, 937
BlpI	GCTNAGC	1 cut(s)	398
BlsI	GCNGC	5 cut(s)	432, 613, 616, 781, 938
BmcAI	AGTACT	1 cut(s)	71
Bme18I	GGWCC	2 cut(s)	20, 266
BmgT120I	GGNCC	2 cut(s)	20, 266
BmiI	GGNNCC	1 cut(s)	923
BmrI	ACTGGG	1 cut(s)	742
BmsI	GCATC	2 cut(s)	337, 426
BmuI	ACTGGG	1 cut(s)	742
BpiI	GAAGAC	1 cut(s)	136
BpmI	CTGGAG	1 cut(s)	610
Bpu10I	CCTNAGC	1 cut(s)	932
Bpu1102I	GCTNAGC	1 cut(s)	398
BpuEI	CTTGAG	2 cut(s)	446, 727
BsaAI	YACGTR	1 cut(s)	76
BsaJI	CCNNGG	1 cut(s)	721
Bsc4I	CCNNNNNNNGG	3 cut(s)	17, 263, 752
Bse1I	ACTGG	3 cut(s)	67, 593, 748
BseDI	CCNNGG	1 cut(s)	721
BseGI	GGATG	5 cut(s)	83, 341, 375, 417, 761
BseLI	CCNNNNNNNGG	3 cut(s)	17, 263, 752
BseMII	CTCAG	2 cut(s)	259, 412
BseNI	ACTGG	3 cut(s)	67, 593, 748
BseXI	GCAGC	3 cut(s)	442, 791, 923
Bsh1236I	CGCG	1 cut(s)	450
BshFI	GGCC	3 cut(s)	131, 212, 611
BshNI	GGYRCC	1 cut(s)	921
BsiHKAI	GWGCWC	1 cut(s)	301
BsiSI	CCGG	1 cut(s)	858
BslFI	GGGAC	1 cut(s)	729
BslI	CCNNNNNNNGG	3 cut(s)	17, 263, 752
BsmAI	GTCTC	1 cut(s)	771
BsmFI	GGGAC	1 cut(s)	729
BsnI	GGCC	3 cut(s)	131, 212, 611
Bsp1286I	GDGCHC	1 cut(s)	301
Bsp1407I	TGTACA	1 cut(s)	319
Bsp143I	GATC	1 cut(s)	7
Bsp1720I	GCTNAGC	1 cut(s)	398
BspACI	CCGC	4 cut(s)	23, 612, 615, 963
BspANI	GGCC	3 cut(s)	131, 212, 611
BspCNI	CTCAG	2 cut(s)	258, 411
BspFNI	CGCG	1 cut(s)	450
BspLI	GGNNCC	1 cut(s)	923
BspMAI	CTGCAG	1 cut(s)	917
BspPI	GGATC	1 cut(s)	2
BspT107I	GGYRCC	1 cut(s)	921
BsrGI	TGTACA	1 cut(s)	319
BsrI	ACTGG	3 cut(s)	67, 593, 748
BssECI	CCNNGG	1 cut(s)	721
BssMI	GATC	1 cut(s)	7
BssSI	CACGAG	1 cut(s)	789
BssT1I	CCWWGG	1 cut(s)	721
Bst2BI	CACGAG	1 cut(s)	789
Bst4CI	ACNGT	1 cut(s)	885
BstAPI	GCANNNNNTGC	2 cut(s)	785, 921
BstAUI	TGTACA	1 cut(s)	319
BstBAI	YACGTR	1 cut(s)	76
BstC8I	GCNNGC	3 cut(s)	366, 387, 941
BstDEI	CTNAG	5 cut(s)	245, 398, 652, 801, 932
BstF5I	GGATG	5 cut(s)	83, 341, 375, 417, 761
BstFNI	CGCG	1 cut(s)	450
BstH2I	RGCGCY	1 cut(s)	543
BstHHI	GCGC	1 cut(s)	542
BstKTI	GATC	1 cut(s)	10
BstMAI	GTCTC	1 cut(s)	771
BstMBI	GATC	1 cut(s)	7
BstMWI	GCNNNNNNNGC	3 cut(s)	539, 785, 921
BstNSI	RCATGY	2 cut(s)	368, 397
BstSFI	CTRYAG	2 cut(s)	867, 913
BstUI	CGCG	1 cut(s)	450
BstV1I	GCAGC	3 cut(s)	442, 791, 923
BstV2I	GAAGAC	1 cut(s)	136
BstXI	CCANNNNNNTGG	1 cut(s)	220
BsuRI	GGCC	3 cut(s)	131, 212, 611
BtsCI	GGATG	5 cut(s)	83, 341, 375, 417, 761
BtsI	GCAGTG	1 cut(s)	910
BtsIMutI	CAGTG	1 cut(s)	910
Cac8I	GCNNGC	3 cut(s)	366, 387, 941
CfoI	GCGC	1 cut(s)	542
Cfr13I	GGNCC	2 cut(s)	20, 266
CseI	GACGC	1 cut(s)	634
Csp6I	GTAC	3 cut(s)	70, 320, 446
CviAII	CATG	5 cut(s)	157, 323, 365, 394, 674
CviQI	GTAC	3 cut(s)	70, 320, 446
DdeI	CTNAG	5 cut(s)	245, 398, 652, 801, 932
DpnI	GATC	1 cut(s)	9
DpnII	GATC	1 cut(s)	7
EaeI	YGGCCR	2 cut(s)	129, 210
Ecl136II	GAGCTC	1 cut(s)	299
Eco130I	CCWWGG	1 cut(s)	721
Eco24I	GRGCYC	1 cut(s)	301
Eco47I	GGWCC	2 cut(s)	20, 266
Eco53kI	GAGCTC	1 cut(s)	299
EcoICRI	GAGCTC	1 cut(s)	299
EcoT14I	CCWWGG	1 cut(s)	721
EcoT38I	GRGCYC	1 cut(s)	301
ErhI	CCWWGG	1 cut(s)	721
FaeI	CATG	5 cut(s)	160, 326, 368, 397, 677
FaqI	GGGAC	1 cut(s)	729
FatI	CATG	5 cut(s)	156, 322, 364, 393, 673
FauI	CCCGC	1 cut(s)	956
Fnu4HI	GCNGC	5 cut(s)	431, 612, 615, 780, 937
FokI	GGATG	5 cut(s)	70, 348, 362, 404, 768
FriOI	GRGCYC	1 cut(s)	301
Fsp4HI	GCNGC	5 cut(s)	431, 612, 615, 780, 937
GlaI	GCGC	1 cut(s)	541
GluI	GCNGC	5 cut(s)	431, 612, 615, 780, 937
GsuI	CTGGAG	1 cut(s)	610
HaeII	RGCGCY	1 cut(s)	543
HaeIII	GGCC	3 cut(s)	131, 212, 611
HapII	CCGG	1 cut(s)	858
HgaI	GACGC	1 cut(s)	634
HhaI	GCGC	1 cut(s)	542
Hin1II	CATG	5 cut(s)	160, 326, 368, 397, 677
Hin6I	GCGC	1 cut(s)	540
HinP1I	GCGC	1 cut(s)	540
HincII	GTYRAC	1 cut(s)	679
HindII	GTYRAC	1 cut(s)	679
HinfI	GANTC	5 cut(s)	112, 664, 702, 764, 772
HpaII	CCGG	1 cut(s)	858
Hpy166II	GTNNAC	1 cut(s)	679
Hpy188I	TCNGA	6 cut(s)	88, 277, 513, 560, 574, 771
Hpy188III	TCNNGA	5 cut(s)	11, 302, 425, 474, 706
Hpy8I	GTNNAC	1 cut(s)	679
Hpy99I	CGWCG	1 cut(s)	155
HpyAV	CCTTC	1 cut(s)	279
HpyCH4III	ACNGT	1 cut(s)	885
HpyCH4IV	ACGT	2 cut(s)	75, 947
HpyCH4V	TGCA	4 cut(s)	364, 389, 779, 915
HpyF10VI	GCNNNNNNNGC	3 cut(s)	539, 785, 921
HpyF3I	CTNAG	5 cut(s)	245, 398, 652, 801, 932
HpySE526I	ACGT	2 cut(s)	75, 947
Hsp92II	CATG	5 cut(s)	160, 326, 368, 397, 677
HspAI	GCGC	1 cut(s)	540
Kzo9I	GATC	1 cut(s)	7
LmnI	GCTCC	3 cut(s)	296, 486, 560
Lsp1109I	GCAGC	3 cut(s)	442, 791, 923
LweI	GCATC	2 cut(s)	337, 426
MaeII	ACGT	2 cut(s)	75, 947
MaeIII	GTNAC	1 cut(s)	879
MalI	GATC	1 cut(s)	9
MboI	GATC	1 cut(s)	7
MboII	GAAGA	3 cut(s)	136, 264, 659
MhlI	GDGCHC	1 cut(s)	301
MlsI	TGGCCA	2 cut(s)	131, 212
MluCI	AATT	3 cut(s)	46, 52, 290
MluI	ACGCGT	1 cut(s)	448
MluNI	TGGCCA	2 cut(s)	131, 212
MlyI	GAGTC	1 cut(s)	773
MmeI	TCCRAC	1 cut(s)	87
MnlI	CCTC	7 cut(s)	11, 252, 267, 333, 681, 692, 850
Mox20I	TGGCCA	2 cut(s)	131, 212
MscI	TGGCCA	2 cut(s)	131, 212
MseI	TTAA	3 cut(s)	648, 863, 902
MslI	CAYNNNNRTG	2 cut(s)	353, 369
Msp20I	TGGCCA	2 cut(s)	131, 212
MspI	CCGG	1 cut(s)	858
MvnI	CGCG	1 cut(s)	450
MwoI	GCNNNNNNNGC	3 cut(s)	539, 785, 921
NdeII	GATC	1 cut(s)	7
NlaIII	CATG	5 cut(s)	160, 326, 368, 397, 677
NlaIV	GGNNCC	1 cut(s)	923
NspI	RCATGY	2 cut(s)	368, 397
PaeI	GCATGC	1 cut(s)	368
PfeI	GAWTC	4 cut(s)	112, 664, 702, 772
PflFI	GACNNNGTC	1 cut(s)	763
PkrI	GCNGC	5 cut(s)	432, 613, 616, 781, 938
PleI	GAGTC	1 cut(s)	772
PpsI	GAGTC	1 cut(s)	772
Ppu21I	YACGTR	1 cut(s)	76
Psp124BI	GAGCTC	1 cut(s)	301
PspN4I	GGNNCC	1 cut(s)	923
PspPI	GGNCC	2 cut(s)	20, 266
PstI	CTGCAG	1 cut(s)	917
PsyI	GACNNNGTC	1 cut(s)	763
RsaI	GTAC	3 cut(s)	71, 321, 447
RsaNI	GTAC	3 cut(s)	70, 320, 446
RseI	CAYNNNNRTG	2 cut(s)	353, 369
SacI	GAGCTC	1 cut(s)	301
SaqAI	TTAA	3 cut(s)	648, 863, 902
SatI	GCNGC	5 cut(s)	431, 612, 615, 780, 937
Sau3AI	GATC	1 cut(s)	7
Sau96I	GGNCC	2 cut(s)	20, 266
ScaI	AGTACT	1 cut(s)	71
SchI	GAGTC	1 cut(s)	773
SduI	GDGCHC	1 cut(s)	301
SfaNI	GCATC	2 cut(s)	337, 426
SfcI	CTRYAG	2 cut(s)	867, 913
SinI	GGWCC	2 cut(s)	20, 266
SmiMI	CAYNNNNRTG	2 cut(s)	353, 369
SmlI	CTYRAG	2 cut(s)	425, 706
SmoI	CTYRAG	2 cut(s)	425, 706
SphI	GCATGC	1 cut(s)	368
Sse9I	AATT	3 cut(s)	46, 52, 290
SsiI	CCGC	4 cut(s)	23, 612, 615, 963
SspI	AATATT	1 cut(s)	520
SstI	GAGCTC	1 cut(s)	301
StyI	CCWWGG	1 cut(s)	721
TaaI	ACNGT	1 cut(s)	885
TaiI	ACGT	2 cut(s)	78, 950
TaqI	TCGA	1 cut(s)	237
TaqII	GACCGA	1 cut(s)	254
TasI	AATT	3 cut(s)	46, 52, 290
TatI	WGTACW	2 cut(s)	69, 319
TauI	GCSGC	2 cut(s)	614, 617
TfiI	GAWTC	4 cut(s)	112, 664, 702, 772
Tru1I	TTAA	3 cut(s)	648, 863, 902
Tru9I	TTAA	3 cut(s)	648, 863, 902
TscAI	CASTG	1 cut(s)	917
TseI	GCWGC	3 cut(s)	430, 779, 936
TspDTI	ATGAA	2 cut(s)	136, 496
TspGWI	ACGGA	1 cut(s)	410
TspRI	CASTG	1 cut(s)	917
Tth111I	GACNNNGTC	1 cut(s)	763
VpaK11BI	GGWCC	2 cut(s)	20, 266
XceI	RCATGY	2 cut(s)	368, 397
XcmI	CCANNNNNNNNNTGG	1 cut(s)	951
ZrmI	AGTACT	1 cut(s)	71

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.