MD04G1069900.v1.1

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007133
Physical Location & Seq
Reverse (-)
9574387 .. 9577603
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1069900.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCGTCGTCGTGGTCGACCGAAGAATCGAATCCCGATCAGCAGCAGCCACCACAACCGCCGCCGCAGCAGCAACAACAATCAGTGAAAGAGTGTGTCCACAAGACCAAGCTCATCCAATTTTTGGGACGCTCCACTCCTATCGTCCTCCAGAACGACAATGGTCCCTGCCCGCTCCTCGCTATCTGTAACGTTCTCTCGCTGAGGAACAGCTTGAATTTGAGTCCAGACACGTCAGAAGTCTCACAGGAGAAACTACTTTCACTCATTGCTGAAAGACTGATTGATTCTAACAGTAATATCGATAATAAAGATGCTGGATATGTAGAAAACCAACAGCAGAACATTGCTGATGCTATTGATCTTCTCCCTCGACTTGCAACGGGGATTGATGTAAATATAAAATTCAGAAGGATAGATGATTTTGAGTTTACTCCAGAGTGTGCCATATTTGACCTGTTAGACATTCCACTATATCATGGTTGGATCGTTGATCCGCAGGTTTGCTTCTTTAGTTCTCAATCCTTATGTAGCATGAGTAGAGTTATTCAAGCACTAATTTTGTTTATTGCAAAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

194

Amino Acids

21.61

Weight (kDa)

4.58

Isoelectric Point (pI)

64.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY_DUB PF04424 33 - 167 2.5e-48 MINDY deubiquitinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 124
AccBSI CCGCTC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 17
AciI CCGC 5 cut(s) 59, 62, 65, 173, 497
AclI AACGTT 1 cut(s) 192
AclWI GGATC 2 cut(s) 488, 494
AcsI RAATTY 2 cut(s) 217, 404
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 2 cut(s) 217, 551
AjiI CACGTC 1 cut(s) 234
AluBI AGCT 2 cut(s) 112, 213
AluI AGCT 2 cut(s) 112, 213
Alw26I GTCTC 1 cut(s) 247
AlwI GGATC 2 cut(s) 488, 494
ApeKI GCWGC 4 cut(s) 43, 46, 67, 70
ApoI RAATTY 2 cut(s) 217, 404
AspS9I GGNCC 1 cut(s) 164
AvaII GGWCC 1 cut(s) 164
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 4 cut(s) 55, 58, 79, 82
BcoDI GTCTC 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 6 cut(s) 44, 47, 62, 65, 68, 71
BlsI GCNGC 6 cut(s) 45, 48, 63, 66, 69, 72
Bme18I GGWCC 1 cut(s) 164
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 166
BmsI GCATC 2 cut(s) 304, 343
BoxI GACNNNNGTC 1 cut(s) 162
BpmI CTGGAG 2 cut(s) 134, 420
Bpu10I CCTNAGC 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse3DI GCAATG 2 cut(s) 267, 345
BseCI ATCGAT 1 cut(s) 303
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 2 cut(s) 267, 345
BseMII CTCAG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 4 cut(s) 55, 58, 79, 82
Bsh1285I CGRYCG 1 cut(s) 21
BshVI ATCGAT 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 141, 150
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 247
BsmFI GGGAC 2 cut(s) 141, 150
Bsp143I GATC 4 cut(s) 37, 361, 486, 493
BspACI CCGC 5 cut(s) 59, 62, 65, 173, 497
BspCNI CTCAG 1 cut(s) 195
BspDI ATCGAT 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 2 cut(s) 488, 494
BsrBI CCGCTC 1 cut(s) 175
BsrDI GCAATG 2 cut(s) 267, 345
BssMI GATC 4 cut(s) 37, 361, 486, 493
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 1 cut(s) 296
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 114
BstKTI GATC 4 cut(s) 40, 364, 489, 496
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 4 cut(s) 37, 361, 486, 493
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 67, 70
BstPAI GACNNNNGTC 1 cut(s) 162
BstV1I GCAGC 4 cut(s) 55, 58, 79, 82
Bsu15I ATCGAT 1 cut(s) 303
BsuTUI ATCGAT 1 cut(s) 303
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 90
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 164
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 1 cut(s) 138
CviAII CATG 2 cut(s) 479, 535
CviJI RGCY 3 cut(s) 49, 112, 213
CviKI_1 RGCY 3 cut(s) 49, 112, 213
DdeI CTNAG 1 cut(s) 203
DpnI GATC 4 cut(s) 39, 363, 488, 495
DpnII GATC 4 cut(s) 37, 361, 486, 493
Eco47I GGWCC 1 cut(s) 164
FaeI CATG 2 cut(s) 482, 538
FaiI YATR 7 cut(s) 324, 401, 449, 475, 480, 529, 536
FaqI GGGAC 2 cut(s) 141, 150
FatI CATG 2 cut(s) 478, 534
FauI CCCGC 1 cut(s) 180
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 6 cut(s) 44, 47, 62, 65, 68, 71
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 6 cut(s) 44, 47, 62, 65, 68, 71
GluI GCNGC 6 cut(s) 44, 47, 62, 65, 68, 71
GsuI CTGGAG 2 cut(s) 134, 420
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 482, 538
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 4 cut(s) 26, 31, 223, 287
Hpy166II GTNNAC 3 cut(s) 18, 100, 432
Hpy188I TCNGA 3 cut(s) 238, 410, 581
Hpy188III TCNNGA 4 cut(s) 35, 151, 227, 437
Hpy8I GTNNAC 3 cut(s) 18, 100, 432
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 2 cut(s) 192, 233
HpyCH4V TGCA 2 cut(s) 380, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 70
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 2 cut(s) 192, 233
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 482, 538
Kzo9I GATC 4 cut(s) 37, 361, 486, 493
LmnI GCTCC 2 cut(s) 137, 180
LpnPI CCDG 8 cut(s) 164, 181, 233, 240, 303, 450, 470, 485
Lsp1109I GCAGC 4 cut(s) 55, 58, 79, 82
LweI GCATC 2 cut(s) 304, 343
MaeII ACGT 2 cut(s) 192, 233
MaeIII GTNAC 1 cut(s) 188
MalI GATC 4 cut(s) 39, 363, 488, 495
MbiI CCGCTC 1 cut(s) 175
MboI GATC 4 cut(s) 37, 361, 486, 493
MboII GAAGA 2 cut(s) 35, 356
MluCI AATT 4 cut(s) 119, 217, 404, 558
MlyI GAGTC 1 cut(s) 232
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 4 cut(s) 158, 188, 198, 381
MwoI GCNNNNNNNGC 2 cut(s) 67, 70
NdeII GATC 4 cut(s) 37, 361, 486, 493
NlaIII CATG 2 cut(s) 482, 538
NlaIV GGNNCC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 3 cut(s) 26, 31, 287
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 6 cut(s) 45, 48, 63, 66, 69, 72
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 162
Psp1406I AACGTT 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 164
SalI GTCGAC 1 cut(s) 16
SatI GCNGC 6 cut(s) 44, 47, 62, 65, 68, 71
Sau3AI GATC 4 cut(s) 37, 361, 486, 493
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 1 cut(s) 232
SetI ASST 6 cut(s) 114, 195, 215, 236, 459, 504
SfaNI GCATC 2 cut(s) 304, 343
SinI GGWCC 1 cut(s) 164
Sse9I AATT 4 cut(s) 119, 217, 404, 558
SsiI CCGC 5 cut(s) 59, 62, 65, 173, 497
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 2 cut(s) 195, 236
TaqI TCGA 4 cut(s) 17, 29, 303, 373
TaqII GACCGA 1 cut(s) 35
TasI AATT 4 cut(s) 119, 217, 404, 558
TauI GCSGC 2 cut(s) 64, 67
TfiI GAWTC 3 cut(s) 26, 31, 287
TscAI CASTG 1 cut(s) 90
TseI GCWGC 4 cut(s) 43, 46, 67, 70
TspRI CASTG 1 cut(s) 90
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 217, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmiI GTMKAC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.