MD06G1071200.v1.1

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007133
Physical Location & Seq
Reverse (-)
17260791 .. 17265902
5112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1071200.v1.1.491

Sequence Viewer

Length: 1728 bp
ATGCCATCTGATATTTACTGTATGGCCTTTGTTTTCGTAATCAATTTTCTAGTGTATGTTGACCAGGATGATAACACTGCCAATGCAATTGGGTCAAAGTCTTATAATGCTCTCATGGGGGAGCTTGTTGCATTGGAAACCCAGAATATGGAGAGTGAGTGTAAGAATACTCCAGAAGAAGATTGCGTTGATTTTGCTGCTGCAACAACTGCAACCCTGGGAGTCCCTTCACCATGCCTTTCTACAACTAGATCTTTCAACAAGTCTCCACATTCAGCTTCTGATGAACCAAGGGCAAGAAAAGGTGACCGTGAAGAAGAAGAGGAGTTAATGAGGGCTTTACAAATGTCTGAGGCCGAATTGACAACCTTAGTTGGTGATCCATGTGCAGTTGTCAGTACTAACAGGGCCCCTCTGTTGATTAGTTCAGATGCAAGTACACTTCCTGAGAAGGTTGTGCCAATAGATTCTCAAGATGAATCAGAGAATCATGCTGCTGTATACAATAATGTCCATCAGTCAGAACTGTCTGTACCAGAAGATTACAATACCTCCAGGAATGAGAGTAGTAATGTGATATCTTTAAGTACTACTCCAGAGCAAACAGCATGTTCCTCGTTGTCAGAGACTGTTAAGGCAGAAAATACTGATCAGCCAACTGATATGAAATCAGATGAGCGTCTTTCCTCTGTTCTGGAAGTGAAAAGCACTACAAATGAATCAGTCAAGATTGAAAGTGGAATTTCTTTTTCTCCGGGGAAAGATACTGAGTCATTGGAGGAGAACTGTACAGATGTGTCAATAGGGGATGAAAAGTTTGAGAGTCAGGCCAAGCTTACACCTGATGCTCATGGACATATAGATAAGCAAAATGAGAGCAACGCGACAGAGGGATGTTGCTCATCTGGTCCAAATGTGGGTTTGAATTCATCTGCTGGCAGAATGCAGCAAAATGAGGCATCAGAAACTTTAGCATCAAGTGGTGAAGGCAGTGAGCCCATATATGAAGGAGAAGAGCGCATTCAGGATTCAGGAACTCCAGTTGTTGAAGACAGAGAGCCTGTATATGAAGGTGAGGTGGTACTCGCAAAACAAGTTGACAAAAGCCCCTTAGATGCAAGGCCCAAGGGTGAAATCACTCCACAACAAGGTGAACTGATCAGGACTTTTTTGAAGAACAATGCTAGTCAGTTGACTTTTTATGGCCTTTTCTGCTTACAAGAGGGTCTTAAAGAGCGTGAGCTATGTGTTTTCTTCCGAAATAATCACTTCAGCACCTTGTTTAAGTTCAATAGTGAACTTTATCTTTTGGCTACTGATCAGGGTTACATAAATCAGCCTGATTTGGTGTGGGAAAAGCTAAATGAGGTCAATGGGAATACATTGTTTATGACAGGCAACTTCAAAGAATTTAAGGTAGAAAGTCATACAAATGACACATGGGATGAAAACAATGCTGTCACCAGCACTGCGGACTATCTTGCTAGCATCGATAGTGCAGCACAAGCAGGATTCGATTTGAATTCTGATATGCAGTTGGCAATTGCTCTGCAGCAGCAGGAGTTTGAACAACCACCGCAGCAGCGTCAAAACTCGCAGCAACCATCTAATAGTGGCAATTCAAGGATGGTTGTAGGTCCCCAGGGGCCAAGAAACAATGCCAGAACTTCATCGGCATCCGCATCTGCAAGACCTGAAGCAAAAAAGGAGAAGTGTACTTTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

576

Amino Acids

62.91

Weight (kDa)

4.42

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY_DUB PF04424 22 - 468 9.1e-36 MINDY deubiquitinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
AccB7I CCANNNNNTGG 1 cut(s) 148
AccI GTMKAC 1 cut(s) 501
AccII CGCG 1 cut(s) 884
AciI CCGC 3 cut(s) 1472, 1577, 1680
AclWI GGATC 1 cut(s) 374
AcsI RAATTY 4 cut(s) 741, 925, 1409, 1522
AcuI CTGAAG 2 cut(s) 1255, 1716
AfaI GTAC 7 cut(s) 400, 439, 534, 589, 790, 1083, 1717
AfiI CCNNNNNNNGG 3 cut(s) 148, 917, 1148
AjnI CCWGG 4 cut(s) 63, 216, 554, 1641
AluBI AGCT 5 cut(s) 124, 278, 835, 1243, 1360
AluI AGCT 5 cut(s) 124, 278, 835, 1243, 1360
Alw26I GTCTC 2 cut(s) 270, 620
AlwI GGATC 1 cut(s) 374
AlwNI CAGNNNCTG 2 cut(s) 281, 629
AoxI GGCC 7 cut(s) 24, 354, 408, 828, 1121, 1204, 1646
ApaI GGGCCC 1 cut(s) 412
ApoI RAATTY 4 cut(s) 741, 925, 1409, 1522
AspLEI GCGC 1 cut(s) 1020
AspS9I GGNCC 6 cut(s) 408, 409, 908, 1122, 1637, 1646
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 8 cut(s) 222, 317, 389, 995, 1085, 1142, 1163, 1453
AsuNHI GCTAGC 1 cut(s) 1484
AvaII GGWCC 2 cut(s) 908, 1637
BaeGI GKGCMC 1 cut(s) 412
BanII GRGCYC 2 cut(s) 412, 999
BbsI GAAGAC 1 cut(s) 1056
BccI CCATC 4 cut(s) 13, 522, 1612, 1621
BcgI CGANNNNNNTGC 2 cut(s) 597, 631
BciT130I CCWGG 4 cut(s) 65, 218, 556, 1643
BclI TGATCA 3 cut(s) 649, 1158, 1318
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 2 cut(s) 270, 620
BfaI CTAG 4 cut(s) 50, 249, 1185, 1485
BfmI CTRYAG 1 cut(s) 1550
BglII AGATCT 1 cut(s) 251
BmcAI AGTACT 2 cut(s) 400, 589
Bme1390I CCNGG 5 cut(s) 65, 218, 556, 756, 1643
Bme18I GGWCC 2 cut(s) 908, 1637
BmgT120I GGNCC 6 cut(s) 408, 409, 908, 1122, 1637, 1646
BmiI GGNNCC 4 cut(s) 410, 411, 1639, 1647
BmrFI CCNGG 5 cut(s) 65, 218, 556, 756, 1643
BmsI GCATC 8 cut(s) 421, 835, 968, 983, 1105, 1497, 1685, 1691
BmtI GCTAGC 1 cut(s) 1488
BpiI GAAGAC 1 cut(s) 1056
BpmI CTGGAG 4 cut(s) 156, 538, 579, 1023
BpuEI CTTGAG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 756
Bsa29I ATCGAT 1 cut(s) 1491
BsaJI CCNNGG 7 cut(s) 216, 217, 290, 755, 1125, 1641, 1642
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 536, 566
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 917, 1148
Bse1I ACTGG 1 cut(s) 1040
BseBI CCWGG 4 cut(s) 65, 218, 556, 1643
BseCI ATCGAT 1 cut(s) 1491
BseDI CCNNGG 7 cut(s) 216, 217, 290, 755, 1125, 1641, 1642
BseGI GGATG 6 cut(s) 73, 814, 899, 1450, 1632, 1676
BseLI CCNNNNNNNGG 3 cut(s) 148, 917, 1148
BseMII CTCAG 3 cut(s) 342, 438, 759
BseNI ACTGG 1 cut(s) 1040
BseRI GAGGAG 2 cut(s) 338, 794
BseSI GKGCMC 1 cut(s) 412
BsgI GTGCAG 2 cut(s) 408, 1518
Bsh1236I CGCG 1 cut(s) 884
BshFI GGCC 7 cut(s) 26, 356, 410, 830, 1123, 1206, 1648
BshVI ATCGAT 1 cut(s) 1491
BsiSI CCGG 1 cut(s) 755
BslFI GGGAC 2 cut(s) 209, 1623
BslI CCNNNNNNNGG 3 cut(s) 148, 917, 1148
BsmAI GTCTC 2 cut(s) 270, 620
BsmFI GGGAC 2 cut(s) 209, 1623
BsmI GAATGC 2 cut(s) 948, 1020
BsnI GGCC 7 cut(s) 26, 356, 410, 830, 1123, 1206, 1648
Bsp120I GGGCCC 1 cut(s) 408
Bsp1286I GDGCHC 2 cut(s) 412, 999
Bsp1407I TGTACA 1 cut(s) 788
Bsp143I GATC 5 cut(s) 251, 379, 649, 1158, 1318
BspACI CCGC 3 cut(s) 1472, 1577, 1680
BspANI GGCC 7 cut(s) 26, 356, 410, 830, 1123, 1206, 1648
BspCNI CTCAG 3 cut(s) 343, 439, 760
BspDI ATCGAT 1 cut(s) 1491
BspFNI CGCG 1 cut(s) 884
BspLI GGNNCC 4 cut(s) 410, 411, 1639, 1647
BspMAI CTGCAG 1 cut(s) 1554
BspOI GCTAGC 1 cut(s) 1488
BspPI GGATC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 1008
BsrGI TGTACA 1 cut(s) 788
BsrI ACTGG 1 cut(s) 1040
BssECI CCNNGG 7 cut(s) 216, 217, 290, 755, 1125, 1641, 1642
BssMI GATC 5 cut(s) 251, 379, 649, 1158, 1318
BssNAI GTATAC 1 cut(s) 502
BssT1I CCWWGG 2 cut(s) 290, 1125
Bst1107I GTATAC 1 cut(s) 502
Bst2UI CCWGG 4 cut(s) 65, 218, 556, 1643
Bst4CI ACNGT 5 cut(s) 20, 311, 528, 631, 788
Bst6I CTCTTC 2 cut(s) 315, 1008
BstAPI GCANNNNNTGC 1 cut(s) 209
BstAUI TGTACA 1 cut(s) 788
BstC8I GCNNGC 2 cut(s) 937, 1486
BstDEI CTNAG 5 cut(s) 351, 370, 447, 768, 1111
BstEII GGTNACC 1 cut(s) 305
BstF5I GGATG 6 cut(s) 73, 814, 899, 1450, 1632, 1676
BstFNI CGCG 1 cut(s) 884
BstHHI GCGC 1 cut(s) 1020
BstKTI GATC 5 cut(s) 254, 382, 652, 1161, 1321
BstMAI GTCTC 2 cut(s) 270, 620
BstMBI GATC 5 cut(s) 251, 379, 649, 1158, 1318
BstMWI GCNNNNNNNGC 3 cut(s) 209, 1212, 1505
BstNI CCWGG 4 cut(s) 65, 218, 556, 1643
BstNSI RCATGY 1 cut(s) 612
BstPI GGTNACC 1 cut(s) 305
BstSCI CCNGG 5 cut(s) 63, 216, 554, 754, 1641
BstSFI CTRYAG 1 cut(s) 1550
BstSLI GKGCMC 1 cut(s) 412
BstUI CGCG 1 cut(s) 884
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BstZ17I GTATAC 1 cut(s) 502
Bsu15I ATCGAT 1 cut(s) 1491
BsuRI GGCC 7 cut(s) 26, 356, 410, 830, 1123, 1206, 1648
BsuTUI ATCGAT 1 cut(s) 1491
BtsCI GGATG 6 cut(s) 73, 814, 899, 1450, 1632, 1676
BtsI GCAGTG 3 cut(s) 75, 997, 1467
BtsIMutI CAGTG 3 cut(s) 75, 997, 1467
Cac8I GCNNGC 2 cut(s) 937, 1486
CaiI CAGNNNCTG 2 cut(s) 281, 629
CfoI GCGC 1 cut(s) 1020
Cfr13I GGNCC 6 cut(s) 408, 409, 908, 1122, 1637, 1646
ClaI ATCGAT 1 cut(s) 1491
CseI GACGC 2 cut(s) 668, 1574
Csp6I GTAC 7 cut(s) 399, 438, 533, 588, 789, 1082, 1716
CviAII CATG 7 cut(s) 115, 234, 384, 491, 609, 851, 1440
CviQI GTAC 7 cut(s) 399, 438, 533, 588, 789, 1082, 1716
DdeI CTNAG 5 cut(s) 351, 370, 447, 768, 1111
DpnI GATC 5 cut(s) 253, 381, 651, 1160, 1320
DpnII GATC 5 cut(s) 251, 379, 649, 1158, 1318
Eam1104I CTCTTC 2 cut(s) 315, 1008
EarI CTCTTC 2 cut(s) 315, 1008
Eco130I CCWWGG 2 cut(s) 290, 1125
Eco24I GRGCYC 2 cut(s) 412, 999
Eco32I GATATC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 908, 1637
Eco57I CTGAAG 2 cut(s) 1255, 1716
Eco91I GGTNACC 1 cut(s) 305
EcoO109I RGGNCCY 3 cut(s) 408, 409, 1637
EcoO65I GGTNACC 1 cut(s) 305
EcoRI GAATTC 2 cut(s) 925, 1522
EcoRII CCWGG 4 cut(s) 63, 216, 554, 1641
EcoRV GATATC 1 cut(s) 579
EcoT14I CCWWGG 2 cut(s) 290, 1125
EcoT38I GRGCYC 2 cut(s) 412, 999
ErhI CCWWGG 2 cut(s) 290, 1125
FaeI CATG 7 cut(s) 118, 237, 387, 494, 612, 854, 1443
FalI AAGNNNNNCTT 2 cut(s) 1212, 1244
FaqI GGGAC 2 cut(s) 209, 1623
FatI CATG 7 cut(s) 114, 233, 383, 490, 608, 850, 1439
FbaI TGATCA 3 cut(s) 649, 1158, 1318
FblI GTMKAC 1 cut(s) 501
FokI GGATG 6 cut(s) 80, 821, 906, 1457, 1639, 1663
FriOI GRGCYC 2 cut(s) 412, 999
FspBI CTAG 4 cut(s) 50, 249, 1185, 1485
GlaI GCGC 1 cut(s) 1019
GsuI CTGGAG 4 cut(s) 156, 538, 579, 1023
HaeIII GGCC 7 cut(s) 26, 356, 410, 830, 1123, 1206, 1648
HapII CCGG 1 cut(s) 755
HgaI GACGC 2 cut(s) 668, 1574
HhaI GCGC 1 cut(s) 1020
Hin1II CATG 7 cut(s) 118, 237, 387, 494, 612, 854, 1443
Hin6I GCGC 1 cut(s) 1018
HinP1I GCGC 1 cut(s) 1018
HincII GTYRAC 3 cut(s) 61, 1099, 1194
HindII GTYRAC 3 cut(s) 61, 1099, 1194
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 9 cut(s) 222, 467, 479, 487, 719, 770, 823, 1028, 1512
HpaII CCGG 1 cut(s) 755
HphI GGTGA 8 cut(s) 222, 317, 389, 995, 1085, 1142, 1163, 1453
Hpy166II GTNNAC 8 cut(s) 61, 440, 502, 1099, 1154, 1194, 1298, 1716
Hpy188III TCNNGA 9 cut(s) 173, 446, 473, 596, 695, 727, 1025, 1032, 1162
Hpy8I GTNNAC 8 cut(s) 61, 440, 502, 1099, 1154, 1194, 1298, 1716
HpyAV CCTTC 5 cut(s) 237, 445, 980, 1001, 1064
HpyCH4III ACNGT 5 cut(s) 20, 311, 528, 631, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 1212, 1505
HpyF3I CTNAG 5 cut(s) 351, 370, 447, 768, 1111
Hsp92II CATG 7 cut(s) 118, 237, 387, 494, 612, 854, 1443
HspAI GCGC 1 cut(s) 1018
Ksp22I TGATCA 3 cut(s) 649, 1158, 1318
Kzo9I GATC 5 cut(s) 251, 379, 649, 1158, 1318
LguI GCTCTTC 1 cut(s) 1008
LmnI GCTCC 1 cut(s) 121
LweI GCATC 8 cut(s) 421, 835, 968, 983, 1105, 1497, 1685, 1691
MaeI CTAG 4 cut(s) 50, 249, 1185, 1485
MaeIII GTNAC 3 cut(s) 305, 1325, 1459
MalI GATC 5 cut(s) 253, 381, 651, 1160, 1320
MboI GATC 5 cut(s) 251, 379, 649, 1158, 1318
MfeI CAATTG 2 cut(s) 87, 1542
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 412, 999
MluCI AATT 9 cut(s) 43, 87, 359, 741, 925, 1409, 1522, 1542, 1618
MlyI GAGTC 3 cut(s) 231, 779, 832
MseI TTAA 6 cut(s) 329, 584, 633, 1230, 1284, 1413
MslI CAYNNNNRTG 1 cut(s) 1431
MspI CCGG 1 cut(s) 755
MspR9I CCNGG 5 cut(s) 65, 218, 556, 756, 1643
MunI CAATTG 2 cut(s) 87, 1542
Mva1269I GAATGC 2 cut(s) 948, 1020
MvaI CCWGG 4 cut(s) 65, 218, 556, 1643
MvnI CGCG 1 cut(s) 884
MwoI GCNNNNNNNGC 3 cut(s) 209, 1212, 1505
NciI CCSGG 1 cut(s) 756
NdeII GATC 5 cut(s) 251, 379, 649, 1158, 1318
NheI GCTAGC 1 cut(s) 1484
NlaIII CATG 7 cut(s) 118, 237, 387, 494, 612, 854, 1443
NlaIV GGNNCC 4 cut(s) 410, 411, 1639, 1647
NmuCI GTSAC 2 cut(s) 305, 1459
NspI RCATGY 1 cut(s) 612
PasI CCCWGGG 2 cut(s) 217, 1642
PciSI GCTCTTC 1 cut(s) 1008
PctI GAATGC 2 cut(s) 948, 1020
PfeI GAWTC 6 cut(s) 467, 479, 487, 719, 1028, 1512
PflMI CCANNNNNTGG 1 cut(s) 148
PfoI TCCNGGA 1 cut(s) 554
PleI GAGTC 3 cut(s) 230, 778, 831
PpsI GAGTC 3 cut(s) 230, 778, 831
PpuMI RGGWCCY 1 cut(s) 1637
PsiI TTATAA 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 1637
Psp6I CCWGG 4 cut(s) 63, 216, 554, 1641
PspEI GGTNACC 1 cut(s) 305
PspGI CCWGG 4 cut(s) 63, 216, 554, 1641
PspN4I GGNNCC 4 cut(s) 410, 411, 1639, 1647
PspOMI GGGCCC 1 cut(s) 408
PspPI GGNCC 6 cut(s) 408, 409, 908, 1122, 1637, 1646
PspPPI RGGWCCY 1 cut(s) 1637
PsrI GAACNNNNNNTAC 2 cut(s) 516, 548
PstI CTGCAG 1 cut(s) 1554
PstNI CAGNNNCTG 2 cut(s) 281, 629
PsuI RGATCY 1 cut(s) 251
RsaI GTAC 7 cut(s) 400, 439, 534, 589, 790, 1083, 1717
RsaNI GTAC 7 cut(s) 399, 438, 533, 588, 789, 1082, 1716
RseI CAYNNNNRTG 1 cut(s) 1431
SapI GCTCTTC 1 cut(s) 1008
SaqAI TTAA 6 cut(s) 329, 584, 633, 1230, 1284, 1413
Sau3AI GATC 5 cut(s) 251, 379, 649, 1158, 1318
Sau96I GGNCC 6 cut(s) 408, 409, 908, 1122, 1637, 1646
ScaI AGTACT 2 cut(s) 400, 589
SchI GAGTC 3 cut(s) 231, 779, 832
ScrFI CCNGG 5 cut(s) 65, 218, 556, 756, 1643
SduI GDGCHC 2 cut(s) 412, 999
SfaNI GCATC 8 cut(s) 421, 835, 968, 983, 1105, 1497, 1685, 1691
SfcI CTRYAG 1 cut(s) 1550
SinI GGWCC 2 cut(s) 908, 1637
SmiMI CAYNNNNRTG 1 cut(s) 1431
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 9 cut(s) 43, 87, 359, 741, 925, 1409, 1522, 1542, 1618
SsiI CCGC 3 cut(s) 1472, 1577, 1680
SspMI CTAG 4 cut(s) 50, 249, 1185, 1485
StyD4I CCNGG 5 cut(s) 63, 216, 554, 754, 1641
StyI CCWWGG 2 cut(s) 290, 1125
TaaI ACNGT 5 cut(s) 20, 311, 528, 631, 788
TaqI TCGA 2 cut(s) 1491, 1515
TasI AATT 9 cut(s) 43, 87, 359, 741, 925, 1409, 1522, 1542, 1618
TatI WGTACW 5 cut(s) 398, 437, 587, 788, 1715
TfiI GAWTC 6 cut(s) 467, 479, 487, 719, 1028, 1512
Tru1I TTAA 6 cut(s) 329, 584, 633, 1230, 1284, 1413
Tru9I TTAA 6 cut(s) 329, 584, 633, 1230, 1284, 1413
TscAI CASTG 3 cut(s) 82, 997, 1474
TseFI GTSAC 2 cut(s) 305, 1459
Tsp45I GTSAC 2 cut(s) 305, 1459
TspRI CASTG 3 cut(s) 82, 997, 1474
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 908, 1637
XapI RAATTY 4 cut(s) 741, 925, 1409, 1522
XceI RCATGY 1 cut(s) 612
XmiI GTMKAC 1 cut(s) 501
XspI CTAG 4 cut(s) 50, 249, 1185, 1485
ZrmI AGTACT 2 cut(s) 400, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.