Rorug07G0056300

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0007133
Physical Location & Seq
Forward (+)
4006423 .. 4012009
5587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0056300.1

Sequence Viewer

Length: 816 bp
ATGGGGAGGGGAAGGGTTCAGTTGAAGCGGATAGAAAACAAGATCAATAGGCAGGTGACTTTCTCGAAGAGAAGGTCTGGGCTGCTCAAGAAGGCGCATGAGATTTCTGTGCTCTGCGATGCTGAGGTCGCTTTGATAGTCTTCTCCACCAAAGGCAAGCTCTTCGAGTACTCGTCTGATTCTTGCATGGAAAGAATCCTGGAAAGGTATGAAAGATACTCATACGCAGAGCGGCAGCTTCTTGGAAATAATCACGATCACGACCACGATCAAGATCAATCAAATGGAAACTGGACTCTAGAACATGCAAAGCTCAAAGCTAGGGTGGAGGTTTTACAGAGAAATCAAAGTCATTTTATGGGGGAAGACCTACAATCCTTAAGTTTGAAAGAGCTTCAGAATTTGGAGCAGCAACTTGATTCTGCTCTGAAGCACATAAGGTCTAGAAAGAACCAACTTATGTACGAATCCATTTCGGTGCTCCAGAAGAAGGATAAGGTGTTGCAGGAGAAAAATAATTTGCTGACAAAAATGGTCAAGGAGAAGGAGAAGGAGAGAGCAGTAGCCGGCAGTGCCCCACAAGCACAGGCACAGGCACAATGGGAGCAGATGCAGAGGCATAGCTTGGACTCCTCCTCCAGCAGTGCCTTTCTACCTCAGGCTTTGCAGTCCTTGAATTTCAGAGGCTCATCTGGAGACTATGATCATGATGAAGAAATAACCCCACCTCCACCTCCGCCTCAACATCTAGCTGCCGCTGCTGCCAACAGTAATACACTCCTGCCGCCTTGGATGCTCCGCCACCTTAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

271

Amino Acids

31.09

Weight (kDa)

8.73

Isoelectric Point (pI)

60.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 9.7e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 91 - 179 3.7e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AccBSI CCGCTC 1 cut(s) 232
AciI CCGC 6 cut(s) 28, 232, 737, 756, 785, 799
AcsI RAATTY 2 cut(s) 400, 676
AcuI CTGAAG 2 cut(s) 380, 449
AfaI GTAC 2 cut(s) 170, 464
AfiI CCNNNNNNNGG 1 cut(s) 490
AflII CTTAAG 1 cut(s) 379
AgsI TTSAA 3 cut(s) 25, 388, 676
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 7 cut(s) 160, 238, 313, 320, 394, 624, 752
AluI AGCT 7 cut(s) 160, 238, 313, 320, 394, 624, 752
Alw21I GWGCWC 2 cut(s) 114, 483
Alw26I GTCTC 1 cut(s) 690
ApeKI GCWGC 6 cut(s) 82, 235, 409, 752, 758, 761
ApoI RAATTY 2 cut(s) 400, 676
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 577
BbsI GAAGAC 2 cut(s) 133, 372
Bbv12I GWGCWC 2 cut(s) 114, 483
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 6 cut(s) 69, 247, 421, 739, 745, 748
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 703
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 4 cut(s) 299, 321, 444, 749
BfrI CTTAAG 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 9 cut(s) 83, 233, 236, 410, 753, 756, 759, 762, 785
BlsI GCNGC 9 cut(s) 84, 234, 237, 411, 754, 757, 760, 763, 786
BmcAI AGTACT 1 cut(s) 170
Bme1390I CCNGG 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 3 cut(s) 109, 600, 783
BpiI GAAGAC 2 cut(s) 133, 372
BpmI CTGGAG 3 cut(s) 467, 622, 714
Bpu10I CCTNAGC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 788
BsaXI ACNNNNNCTCC 4 cut(s) 620, 650, 712, 742
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 1 cut(s) 296
Bse21I CCTNAGG 1 cut(s) 657
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 788
BseGI GGATG 1 cut(s) 798
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 114, 671
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 2 cut(s) 622, 625
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 6 cut(s) 69, 247, 421, 739, 745, 748
BsiHKAI GWGCWC 2 cut(s) 114, 483
BsiSI CCGG 1 cut(s) 567
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 1 cut(s) 690
Bsp1286I GDGCHC 3 cut(s) 114, 483, 577
Bsp143I GATC 5 cut(s) 42, 256, 268, 274, 703
BspACI CCGC 6 cut(s) 28, 232, 737, 756, 785, 799
BspCNI CTCAG 2 cut(s) 115, 670
BspHI TCATGA 1 cut(s) 706
BspMI ACCTGC 1 cut(s) 43
BspQI GCTCTTC 1 cut(s) 167
BspTI CTTAAG 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 1 cut(s) 788
BssMI GATC 5 cut(s) 42, 256, 268, 274, 703
BssT1I CCWWGG 1 cut(s) 788
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 770
Bst6I CTCTTC 2 cut(s) 62, 167
BstAFI CTTAAG 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 158, 568
BstDEI CTNAG 2 cut(s) 123, 657
BstF5I GGATG 1 cut(s) 798
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 5 cut(s) 45, 259, 271, 277, 706
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 5 cut(s) 42, 256, 268, 274, 703
BstMWI GCNNNNNNNGC 6 cut(s) 128, 572, 581, 758, 761, 793
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 6 cut(s) 69, 247, 421, 739, 745, 748
BstV2I GAAGAC 2 cut(s) 133, 372
Bsu36I CCTNAGG 1 cut(s) 657
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 1 cut(s) 798
BtsI GCAGTG 2 cut(s) 577, 649
BtsIMutI CAGTG 2 cut(s) 577, 649
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 2 cut(s) 158, 568
CciI TCATGA 1 cut(s) 706
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 566
Csp6I GTAC 2 cut(s) 169, 463
CviAII CATG 4 cut(s) 98, 187, 305, 707
CviQI GTAC 2 cut(s) 169, 463
DdeI CTNAG 2 cut(s) 123, 657
DpnI GATC 5 cut(s) 44, 258, 270, 276, 705
DpnII GATC 5 cut(s) 42, 256, 268, 274, 703
Eam1104I CTCTTC 2 cut(s) 62, 167
EarI CTCTTC 2 cut(s) 62, 167
EciI GGCGGA 2 cut(s) 726, 788
Eco130I CCWWGG 1 cut(s) 788
Eco57I CTGAAG 2 cut(s) 380, 449
Eco81I CCTNAGG 1 cut(s) 657
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 788
ErhI CCWWGG 1 cut(s) 788
FaeI CATG 4 cut(s) 101, 190, 308, 710
FatI CATG 4 cut(s) 97, 186, 304, 706
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 9 cut(s) 83, 233, 236, 410, 753, 756, 759, 762, 785
FokI GGATG 1 cut(s) 805
Fsp4HI GCNGC 9 cut(s) 83, 233, 236, 410, 753, 756, 759, 762, 785
FspBI CTAG 4 cut(s) 299, 321, 444, 749
GlaI GCGC 1 cut(s) 96
GluI GCNGC 9 cut(s) 83, 233, 236, 410, 753, 756, 759, 762, 785
GsuI CTGGAG 3 cut(s) 467, 622, 714
HapII CCGG 1 cut(s) 567
HhaI GCGC 1 cut(s) 97
Hin1II CATG 4 cut(s) 101, 190, 308, 710
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 6 cut(s) 179, 195, 295, 419, 467, 629
HpaII CCGG 1 cut(s) 567
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 4 cut(s) 178, 399, 429, 683
HpyAV CCTTC 6 cut(s) 6, 66, 85, 484, 538, 544
HpyCH4III ACNGT 1 cut(s) 770
HpyCH4V TGCA 5 cut(s) 186, 308, 505, 613, 667
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 572, 581, 758, 761, 793
HpyF3I CTNAG 2 cut(s) 123, 657
Hsp92II CATG 4 cut(s) 101, 190, 308, 710
HspAI GCGC 1 cut(s) 95
KroI GCCGGC 1 cut(s) 566
KroNI GCCGGC 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 5 cut(s) 42, 256, 268, 274, 703
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 4 cut(s) 406, 486, 604, 801
Lsp1109I GCAGC 6 cut(s) 69, 247, 421, 739, 745, 748
LweI GCATC 3 cut(s) 109, 600, 783
MaeI CTAG 4 cut(s) 299, 321, 444, 749
MaeIII GTNAC 1 cut(s) 55
MalI GATC 5 cut(s) 44, 258, 270, 276, 705
MbiI CCGCTC 1 cut(s) 232
MboI GATC 5 cut(s) 42, 256, 268, 274, 703
MboII GAAGA 6 cut(s) 79, 133, 154, 377, 499, 725
MhlI GDGCHC 3 cut(s) 114, 483, 577
MluCI AATT 3 cut(s) 400, 517, 676
MlyI GAGTC 2 cut(s) 289, 623
MroNI GCCGGC 1 cut(s) 566
MseI TTAA 2 cut(s) 380, 807
MslI CAYNNNNRTG 2 cut(s) 476, 807
MspA1I CMGCKG 1 cut(s) 758
MspCI CTTAAG 1 cut(s) 379
MspI CCGG 1 cut(s) 567
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 6 cut(s) 128, 572, 581, 758, 761, 793
NaeI GCCGGC 1 cut(s) 568
NdeII GATC 5 cut(s) 42, 256, 268, 274, 703
NgoMIV GCCGGC 1 cut(s) 566
NlaIII CATG 4 cut(s) 101, 190, 308, 710
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 308
PagI TCATGA 1 cut(s) 706
PaqCI CACCTGC 1 cut(s) 43
PciSI GCTCTTC 1 cut(s) 167
PdiI GCCGGC 1 cut(s) 568
PfeI GAWTC 4 cut(s) 179, 195, 419, 467
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 9 cut(s) 84, 234, 237, 411, 754, 757, 760, 763, 786
PleI GAGTC 2 cut(s) 289, 623
PpsI GAGTC 2 cut(s) 289, 623
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
RsaI GTAC 2 cut(s) 170, 464
RsaNI GTAC 2 cut(s) 169, 463
RseI CAYNNNNRTG 2 cut(s) 476, 807
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 380, 807
SatI GCNGC 9 cut(s) 83, 233, 236, 410, 753, 756, 759, 762, 785
Sau3AI GATC 5 cut(s) 42, 256, 268, 274, 703
ScaI AGTACT 1 cut(s) 170
SchI GAGTC 2 cut(s) 289, 623
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 3 cut(s) 114, 483, 577
SfaNI GCATC 3 cut(s) 109, 600, 783
SmiMI CAYNNNNRTG 2 cut(s) 476, 807
SmlI CTYRAG 2 cut(s) 86, 379
SmoI CTYRAG 2 cut(s) 86, 379
Sse9I AATT 3 cut(s) 400, 517, 676
SsiI CCGC 6 cut(s) 28, 232, 737, 756, 785, 799
SspMI CTAG 4 cut(s) 299, 321, 444, 749
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 788
TaaI ACNGT 1 cut(s) 770
TaqI TCGA 2 cut(s) 65, 165
TasI AATT 3 cut(s) 400, 517, 676
TatI WGTACW 1 cut(s) 168
TauI GCSGC 3 cut(s) 235, 758, 787
TfiI GAWTC 4 cut(s) 179, 195, 419, 467
Tru1I TTAA 2 cut(s) 380, 807
Tru9I TTAA 2 cut(s) 380, 807
TscAI CASTG 2 cut(s) 577, 649
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 6 cut(s) 82, 235, 409, 752, 758, 761
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 225, 726
TspRI CASTG 2 cut(s) 577, 649
Vha464I CTTAAG 1 cut(s) 379
XapI RAATTY 2 cut(s) 400, 676
XbaI TCTAGA 2 cut(s) 298, 443
XceI RCATGY 1 cut(s) 308
XspI CTAG 4 cut(s) 299, 321, 444, 749
ZrmI AGTACT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.