Rh7DG184400

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007133
Physical Location & Seq
Reverse (-)
17147621 .. 17154898
7278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG184400.1

Sequence Viewer

Length: 1944 bp
ATGGCTTCGTCGGAGGAGGAACCACCGCAGCAGAGCCAAGAACCACCGCAGCAGCCACAGTCAGTGAAAGACTGTCTTCACAAGACCAAGCTCATTCAATTCTTGGGCCGCACCGCCCCTATCGTCCTCCAGAACGACAATGGTCCCTGCCCTCTCCTCGCCATCTGTAATGTTCTCTCCCTGAGAAACAACCTGAATTTGAGTGCAGACACTACAGAAGTCTCTCAAGAAAAGTTACTCTCACTCGTTGCTGAAAGATTAATTGATTCTAACAGTAATATTGATAATAAAGATGCTGGCTATGTAGAAAACCAACAGCAGAATATAGCTGATGCTATCGATCTACTCCCTCGACTTGCAACCGGAATCGATGTTAACATAAAATTCAGAAGGATAAGTGATTTTGAGTTTACTCCAGAATGTGCAATATTTGATCTGTTAGACATCCCACTATATCATGGCTGGATAGTTGATCCCCAGGACCATGAGGCTGCTAATGCAATTGGGTCGAAGTCCTATAACACTCTTACGGGGGAGCTTGTTGCACTGGAAACACAAAATATGGAGAGTGAGTCTAAGGGGAAGAATATTCCTCAAGAAGATTGTGTTGATTTTGTTGCTGCAACAACTGCAACACTTGGTGTACCTTCCCCAAGCCTTTCTAAAACTAGATCTTTTGATGAGTCTCCACATTCAGCTTCTGATGAACCAAAGGAAAGGAAAGGAGACCGTGAAGAAGAAGCAGAGTTGCTGATGGCTTTACAAATGTCTGAGGCTGAGCTTCCGACTGCAGTGGGTGATCCATTTGCAGCAAGTATTGACACACCGCCTCTGTTAGTTAGTTCAGATGAAAGTACTCATCTTGAGAAGGTTATGCTTGTAGGTTCTGCAGATAAATCCGAAAAACATGATGCTGAATGCAGTAATTCTCATCAGTCAGAACTGTCTGTACCAGATAACATTGCCCCCAGGAACGAAAGTAGTGATCTGATATCTTTTGAAACTGCTTCAGGGCAAACAGCATGTTCATCCCGGTTGGAGACGACTGAGGTGAACAATAATGGTCAGCGAACTTCTGTGAAATCAGAAGATCTTCCTAAGGATCTGGTTACAAAAAGCACAGGAAACGAATCAGACCAGATTAAAGGTGCAATTTCTTTTAGTCCTGAGAGAAATAATGAATCCATAGATGAGAACCATACAGATGTTTCAAAAGGGGGTGAAAAATTTGAGGATCAGGTCAAGCTTGCGACTAATGCCGATGGAATTGTAGAAATAAATAAGCAAATTGGGAGCAATATGACAGAGGTTTCTTGCTTATCTGCTTCAAATGTGGGTTCAGATTCATCTAGTGGCAGAATGCTGGAAATTGATGAATCGGAAACTTTGACATCAAGTGGTGAAGGCAGTGAGCCCATCTATGAAGGAGAGGAACGTATTTTGGACTCAGGCACAATTGTTTCTGAACAAAGAGAGCCTGTATATGAAGGTGAGGCAGTGCTTGCAAAGCAAGCAGACAAAGGGATGCTAGATGCAAGGTCAAAGGATGAAATCACTCCACAAGAAGGGGAATTGATCAGGAATTTTTTGAAGAACAATGCTAGTCAATTGACTTTCTACGGCCTTTTCTGCTTACAAGAGGGCCTTAAAGAGCGGGAGCTCTGTGTTTTCTTTCGAAACAACCACTTCAGTACCATGTTTAAGATCAATGGAGAGCTTTATCTTTTGGCTACTGATCAGGGTTACCTGAATCAGCATGATTTGGTGTGGGAAAAGCTTAATGAGGTGAATGGAGATACATTGTTCATGACTGGCAACTTCAAGGAATTTAAAGTTGAAAGCCAATCAAATGAAAATTGGGATGAAAATAATGCTGTTACCAGCACTGCGGACTACCTTGCGAGCATTGATAGTGCAGCTTCAGCAGGCTTCGATCTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

647

Amino Acids

70.89

Weight (kDa)

4.38

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY_DUB PF04424 27 - 200 1.2e-54 MINDY deubiquitinase
MINDY_DUB PF04424 502 - 607 1.8e-37 MINDY deubiquitinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1654
AciI CCGC 7 cut(s) 26, 47, 109, 114, 827, 1654, 1889
AclWI GGATC 4 cut(s) 467, 794, 1110, 1242
AcsI RAATTY 5 cut(s) 196, 383, 1226, 1582, 1826
AcuI CTGAAG 3 cut(s) 993, 1672, 1905
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 4 cut(s) 645, 856, 951, 1693
AfiI CCNNNNNNNGG 1 cut(s) 1565
AgsI TTSAA 7 cut(s) 98, 1001, 1212, 1329, 1591, 1822, 1838
AjnI CCWGG 2 cut(s) 477, 968
Alw21I GWGCWC 1 cut(s) 1662
Alw26I GTCTC 4 cut(s) 226, 690, 720, 1034
AlwI GGATC 4 cut(s) 467, 794, 1110, 1242
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 3 cut(s) 106, 1621, 1642
ApeKI GCWGC 7 cut(s) 28, 49, 52, 491, 620, 809, 1916
ApoI RAATTY 5 cut(s) 196, 383, 1226, 1582, 1826
AseI ATTAAT 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 1092
AspS9I GGNCC 4 cut(s) 106, 143, 481, 1642
AsuC2I CCSGG 1 cut(s) 1033
AsuHPI GGTGA 6 cut(s) 809, 1063, 1232, 1412, 1502, 1798
AsuII TTCGAA 1 cut(s) 1675
AvaII GGWCC 2 cut(s) 143, 481
AxyI CCTNAGG 1 cut(s) 1098
BaeI ACNNNNGTAYC 2 cut(s) 1675, 1708
BanII GRGCYC 2 cut(s) 1416, 1662
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 1662
BbvI GCAGC 7 cut(s) 40, 61, 64, 478, 607, 821, 1928
BccI CCATC 4 cut(s) 170, 748, 1256, 1424
BceAI ACGGC 1 cut(s) 1636
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciT130I CCWGG 2 cut(s) 479, 970
BclI TGATCA 2 cut(s) 1575, 1735
BcnI CCSGG 1 cut(s) 1033
BcoDI GTCTC 4 cut(s) 226, 690, 720, 1034
BfaI CTAG 4 cut(s) 669, 1350, 1529, 1602
BfmI CTRYAG 3 cut(s) 213, 789, 888
BglII AGATCT 2 cut(s) 671, 1090
BisI GCNGC 8 cut(s) 29, 50, 53, 109, 492, 621, 810, 1917
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 8 cut(s) 30, 51, 54, 110, 493, 622, 811, 1918
BmcAI AGTACT 1 cut(s) 856
Bme1390I CCNGG 3 cut(s) 479, 970, 1033
Bme18I GGWCC 2 cut(s) 143, 481
BmgT120I GGNCC 4 cut(s) 106, 143, 481, 1642
BmiI GGNNCC 2 cut(s) 21, 145
BmrFI CCNGG 3 cut(s) 479, 970, 1033
BmsI GCATC 5 cut(s) 283, 322, 901, 1515, 1522
BoxI GACNNNNGTC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 2 cut(s) 113, 399
Bpu1102I GCTNAGC 1 cut(s) 777
Bpu14I TTCGAA 1 cut(s) 1675
BpuEI CTTGAG 3 cut(s) 210, 579, 884
BpuMI CCSGG 1 cut(s) 1033
Bsa29I ATCGAT 2 cut(s) 339, 369
BsaI GGTCTC 1 cut(s) 720
BsaJI CCNNGG 2 cut(s) 477, 968
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 2 cut(s) 557, 587
Bsc4I CCNNNNNNNGG 1 cut(s) 1565
Bse1I ACTGG 2 cut(s) 552, 1816
Bse21I CCTNAGG 1 cut(s) 1098
Bse3DI GCAATG 1 cut(s) 960
BseBI CCWGG 2 cut(s) 479, 970
BseCI ATCGAT 2 cut(s) 339, 369
BseDI CCNNGG 2 cut(s) 477, 968
BseGI GGATG 5 cut(s) 444, 1028, 1530, 1552, 1867
BseLI CCNNNNNNNGG 1 cut(s) 1565
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 6 cut(s) 173, 762, 768, 1038, 1158, 1461
BseNI ACTGG 2 cut(s) 552, 1816
BseRI GAGGAG 2 cut(s) 29, 146
BseXI GCAGC 7 cut(s) 40, 61, 64, 478, 607, 821, 1928
BsgI GTGCAG 2 cut(s) 225, 1935
BshFI GGCC 3 cut(s) 108, 1623, 1644
BshVI ATCGAT 2 cut(s) 339, 369
BsiHKAI GWGCWC 1 cut(s) 1662
BsiSI CCGG 2 cut(s) 363, 1033
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 1 cut(s) 1565
BsmAI GTCTC 4 cut(s) 226, 690, 720, 1034
BsmBI CGTCTC 1 cut(s) 1034
BsmFI GGGAC 1 cut(s) 129
BsmI GAATGC 2 cut(s) 923, 1365
BsnI GGCC 3 cut(s) 108, 1623, 1644
Bso31I GGTCTC 1 cut(s) 720
Bsp119I TTCGAA 1 cut(s) 1675
Bsp1286I GDGCHC 2 cut(s) 1416, 1662
Bsp1720I GCTNAGC 1 cut(s) 777
BspACI CCGC 7 cut(s) 26, 47, 109, 114, 827, 1654, 1889
BspANI GGCC 3 cut(s) 108, 1623, 1644
BspCNI CTCAG 6 cut(s) 174, 763, 769, 1039, 1159, 1460
BspDI ATCGAT 2 cut(s) 339, 369
BspHI TCATGA 1 cut(s) 1806
BspLI GGNNCC 2 cut(s) 21, 145
BspMAI CTGCAG 2 cut(s) 793, 892
BspPI GGATC 4 cut(s) 467, 794, 1110, 1242
BspT104I TTCGAA 1 cut(s) 1675
BspTNI GGTCTC 1 cut(s) 720
BsrBI CCGCTC 1 cut(s) 1654
BsrDI GCAATG 1 cut(s) 960
BsrI ACTGG 2 cut(s) 552, 1816
BssECI CCNNGG 2 cut(s) 477, 968
Bst2UI CCWGG 2 cut(s) 479, 970
Bst4CI ACNGT 5 cut(s) 60, 74, 275, 731, 945
BstAPI GCANNNNNTGC 2 cut(s) 629, 1502
BstBI TTCGAA 1 cut(s) 1675
BstC8I GCNNGC 6 cut(s) 298, 1248, 1503, 1512, 1903, 1927
BstDEI CTNAG 8 cut(s) 182, 576, 771, 777, 1047, 1098, 1167, 1447
BstEII GGTNACC 1 cut(s) 1742
BstF5I GGATG 5 cut(s) 444, 1028, 1530, 1552, 1867
BstMAI GTCTC 4 cut(s) 226, 690, 720, 1034
BstMWI GCNNNNNNNGC 8 cut(s) 497, 629, 1256, 1502, 1507, 1511, 1629, 1922
BstNI CCWGG 2 cut(s) 479, 970
BstNSI RCATGY 1 cut(s) 1026
BstPAI GACNNNNGTC 1 cut(s) 141
BstPI GGTNACC 1 cut(s) 1742
BstSCI CCNGG 3 cut(s) 477, 968, 1031
BstSFI CTRYAG 3 cut(s) 213, 789, 888
BstV1I GCAGC 7 cut(s) 40, 61, 64, 478, 607, 821, 1928
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 3 cut(s) 671, 1090, 1102
BstYI RGATCY 3 cut(s) 671, 1090, 1102
Bsu15I ATCGAT 2 cut(s) 339, 369
Bsu36I CCTNAGG 1 cut(s) 1098
BsuRI GGCC 3 cut(s) 108, 1623, 1644
BsuTUI ATCGAT 2 cut(s) 339, 369
BtsCI GGATG 5 cut(s) 444, 1028, 1530, 1552, 1867
BtsI GCAGTG 4 cut(s) 798, 1414, 1503, 1884
BtsIMutI CAGTG 6 cut(s) 69, 545, 798, 1414, 1503, 1884
Cac8I GCNNGC 6 cut(s) 298, 1248, 1503, 1512, 1903, 1927
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 1806
Cfr13I GGNCC 4 cut(s) 106, 143, 481, 1642
ClaI ATCGAT 2 cut(s) 339, 369
Csp6I GTAC 4 cut(s) 644, 855, 950, 1692
CviAII CATG 7 cut(s) 458, 485, 908, 1023, 1696, 1757, 1807
CviQI GTAC 4 cut(s) 644, 855, 950, 1692
DdeI CTNAG 8 cut(s) 182, 576, 771, 777, 1047, 1098, 1167, 1447
DraI TTTAAA 1 cut(s) 1831
DraIII CACNNNGTG 1 cut(s) 641
Ecl136II GAGCTC 1 cut(s) 1660
Eco24I GRGCYC 2 cut(s) 1416, 1662
Eco31I GGTCTC 1 cut(s) 720
Eco32I GATATC 1 cut(s) 993
Eco47I GGWCC 2 cut(s) 143, 481
Eco53kI GAGCTC 1 cut(s) 1660
Eco57I CTGAAG 3 cut(s) 993, 1672, 1905
Eco81I CCTNAGG 1 cut(s) 1098
Eco91I GGTNACC 1 cut(s) 1742
EcoICRI GAGCTC 1 cut(s) 1660
EcoO109I RGGNCCY 1 cut(s) 1642
EcoO65I GGTNACC 1 cut(s) 1742
EcoRII CCWGG 2 cut(s) 477, 968
EcoRV GATATC 1 cut(s) 993
EcoT38I GRGCYC 2 cut(s) 1416, 1662
Esp3I CGTCTC 1 cut(s) 1034
FaeI CATG 7 cut(s) 461, 488, 911, 1026, 1699, 1760, 1810
FalI AAGNNNNNCTT 4 cut(s) 60, 92, 1629, 1661
FaqI GGGAC 1 cut(s) 129
FatI CATG 7 cut(s) 457, 484, 907, 1022, 1695, 1756, 1806
FauI CCCGC 1 cut(s) 1647
FbaI TGATCA 2 cut(s) 1575, 1735
Fnu4HI GCNGC 8 cut(s) 29, 50, 53, 109, 492, 621, 810, 1917
FokI GGATG 5 cut(s) 431, 1015, 1537, 1559, 1874
FriOI GRGCYC 2 cut(s) 1416, 1662
Fsp4HI GCNGC 8 cut(s) 29, 50, 53, 109, 492, 621, 810, 1917
FspBI CTAG 4 cut(s) 669, 1350, 1529, 1602
GluI GCNGC 8 cut(s) 29, 50, 53, 109, 492, 621, 810, 1917
GsuI CTGGAG 2 cut(s) 113, 399
HaeIII GGCC 3 cut(s) 108, 1623, 1644
HapII CCGG 2 cut(s) 363, 1033
Hin1II CATG 7 cut(s) 461, 488, 911, 1026, 1699, 1760, 1810
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 1244, 1775
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 2 cut(s) 363, 1033
HphI GGTGA 6 cut(s) 809, 1063, 1232, 1412, 1502, 1798
Hpy166II GTNNAC 4 cut(s) 376, 411, 644, 1054
Hpy188III TCNNGA 8 cut(s) 130, 227, 416, 596, 863, 1166, 1579, 1807
Hpy8I GTNNAC 4 cut(s) 376, 411, 644, 1054
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 7 cut(s) 384, 657, 862, 1397, 1418, 1481, 1559
HpyCH4III ACNGT 5 cut(s) 60, 74, 275, 731, 945
HpyCH4IV ACGT 1 cut(s) 1435
HpyF10VI GCNNNNNNNGC 8 cut(s) 497, 629, 1256, 1502, 1507, 1511, 1629, 1922
HpyF3I CTNAG 8 cut(s) 182, 576, 771, 777, 1047, 1098, 1167, 1447
HpySE526I ACGT 1 cut(s) 1435
Hsp92II CATG 7 cut(s) 461, 488, 911, 1026, 1699, 1760, 1810
Ksp22I TGATCA 2 cut(s) 1575, 1735
KspAI GTTAAC 1 cut(s) 376
LmnI GCTCC 3 cut(s) 535, 1293, 1657
Lsp1109I GCAGC 7 cut(s) 40, 61, 64, 478, 607, 821, 1928
LweI GCATC 5 cut(s) 283, 322, 901, 1515, 1522
MaeI CTAG 4 cut(s) 669, 1350, 1529, 1602
MaeII ACGT 1 cut(s) 1435
MaeIII GTNAC 4 cut(s) 234, 1108, 1742, 1876
MbiI CCGCTC 1 cut(s) 1654
MboII GAAGA 8 cut(s) 68, 595, 611, 746, 749, 1085, 1100, 1603
MfeI CAATTG 3 cut(s) 501, 1455, 1607
MflI RGATCY 3 cut(s) 671, 1090, 1102
MhlI GDGCHC 2 cut(s) 1416, 1662
MlyI GAGTC 3 cut(s) 581, 692, 1439
MmeI TCCRAC 2 cut(s) 809, 1017
MroXI GAANNNNTTC 1 cut(s) 1092
MseI TTAA 7 cut(s) 260, 375, 1143, 1647, 1701, 1779, 1830
MslI CAYNNNNRTG 1 cut(s) 1203
MspI CCGG 2 cut(s) 363, 1033
MspR9I CCNGG 3 cut(s) 479, 970, 1033
MunI CAATTG 3 cut(s) 501, 1455, 1607
Mva1269I GAATGC 2 cut(s) 923, 1365
MvaI CCWGG 2 cut(s) 479, 970
MwoI GCNNNNNNNGC 8 cut(s) 497, 629, 1256, 1502, 1507, 1511, 1629, 1922
NciI CCSGG 1 cut(s) 1033
NlaIII CATG 7 cut(s) 461, 488, 911, 1026, 1699, 1760, 1810
NlaIV GGNNCC 2 cut(s) 21, 145
NspI RCATGY 1 cut(s) 1026
NspV TTCGAA 1 cut(s) 1675
PagI TCATGA 1 cut(s) 1806
PctI GAATGC 2 cut(s) 923, 1365
PdmI GAANNNNTTC 1 cut(s) 1092
PfeI GAWTC 7 cut(s) 266, 366, 1130, 1181, 1343, 1376, 1750
PkrI GCNGC 8 cut(s) 30, 51, 54, 110, 493, 622, 811, 1918
PleI GAGTC 3 cut(s) 580, 691, 1439
PpsI GAGTC 3 cut(s) 580, 691, 1439
PshAI GACNNNNGTC 1 cut(s) 141
PshBI ATTAAT 1 cut(s) 260
Psp124BI GAGCTC 1 cut(s) 1662
Psp6I CCWGG 2 cut(s) 477, 968
PspEI GGTNACC 1 cut(s) 1742
PspGI CCWGG 2 cut(s) 477, 968
PspN4I GGNNCC 2 cut(s) 21, 145
PspPI GGNCC 4 cut(s) 106, 143, 481, 1642
PsrI GAACNNNNNNTAC 2 cut(s) 933, 965
PstI CTGCAG 2 cut(s) 793, 892
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 3 cut(s) 671, 1090, 1102
RsaI GTAC 4 cut(s) 645, 856, 951, 1693
RsaNI GTAC 4 cut(s) 644, 855, 950, 1692
RseI CAYNNNNRTG 1 cut(s) 1203
SacI GAGCTC 1 cut(s) 1662
SaqAI TTAA 7 cut(s) 260, 375, 1143, 1647, 1701, 1779, 1830
SatI GCNGC 8 cut(s) 29, 50, 53, 109, 492, 621, 810, 1917
Sau96I GGNCC 4 cut(s) 106, 143, 481, 1642
ScaI AGTACT 1 cut(s) 856
SchI GAGTC 3 cut(s) 581, 692, 1439
ScrFI CCNGG 3 cut(s) 479, 970, 1033
SduI GDGCHC 2 cut(s) 1416, 1662
SfaNI GCATC 5 cut(s) 283, 322, 901, 1515, 1522
SfcI CTRYAG 3 cut(s) 213, 789, 888
SfuI TTCGAA 1 cut(s) 1675
SinI GGWCC 2 cut(s) 143, 481
SmiMI CAYNNNNRTG 1 cut(s) 1203
SmlI CTYRAG 3 cut(s) 225, 594, 863
SmoI CTYRAG 3 cut(s) 225, 594, 863
SsiI CCGC 7 cut(s) 26, 47, 109, 114, 827, 1654, 1889
SspI AATATT 3 cut(s) 280, 429, 589
SspMI CTAG 4 cut(s) 669, 1350, 1529, 1602
SstI GAGCTC 1 cut(s) 1662
StyD4I CCNGG 3 cut(s) 477, 968, 1031
TaaI ACNGT 5 cut(s) 60, 74, 275, 731, 945
TaiI ACGT 1 cut(s) 1438
TaqI TCGA 6 cut(s) 339, 352, 369, 509, 1675, 1932
TatI WGTACW 1 cut(s) 854
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 7 cut(s) 266, 366, 1130, 1181, 1343, 1376, 1750
Tru1I TTAA 7 cut(s) 260, 375, 1143, 1647, 1701, 1779, 1830
Tru9I TTAA 7 cut(s) 260, 375, 1143, 1647, 1701, 1779, 1830
TscAI CASTG 6 cut(s) 69, 552, 798, 1414, 1503, 1891
TseI GCWGC 7 cut(s) 28, 49, 52, 491, 620, 809, 1916
TspRI CASTG 6 cut(s) 69, 552, 798, 1414, 1503, 1891
VpaK11BI GGWCC 2 cut(s) 143, 481
VspI ATTAAT 1 cut(s) 260
XapI RAATTY 5 cut(s) 196, 383, 1226, 1582, 1826
XceI RCATGY 1 cut(s) 1026
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 4 cut(s) 669, 1350, 1529, 1602
ZrmI AGTACT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.