MD04G1110300.v1.1

Protein ALUMINUM SENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013110
Physical Location & Seq
Reverse (-)
19489712 .. 19491675
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1110300.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCATCGCTCCTCTCCACCACCGCACAAAACCACACAGACATTGCACCGTCCAATTTCATACAAGGCCTATTTGCTGCCCAAGTTGTTCTTCCCTTACCTGAGTTTGTGGGCAACATGGACTTGTCATGGATGGTGGACTTTCTCAAGGGCATGGTGAAGCCGCTGGCGGCGACGGTGGTGGTGCTCATGGCGGTGGCCTTGTCGTACATGCAGAAACTGGGATTGGGTAGAGAAATGATTTACTCCATTGCTAGGGCCTTCATTCAGCTCTCGATTATCGGGTTTGTTCTTCAGTTCATCTTCACTCGGGACAATGCCGTTTGGATCGTTCTGGCTTACCTTTTCATGGTGACTGTTGCTGGTTATACAGCAGGGCAAAGGGCTAAGCATGTTCCTAGAGGGAAGTATGTTGCTGGGGCTTCTATATTGGCTGGAACTTCAGTCACAATGTTGCTACTTGTGCTGCTGAGCGTGTTCCCCTTCACTCCAAGATATATTATCCCTGTTGCGGGAATGATGGTCGGAAATTCAATGACGGTAACTGGGGTTACGATGAAAAGACTTCGAGATGATATTCGAAGTCAAATGAACCTGGTAGAGACAGCGTTGGCTCTCGGTGCAACACCGCGCCAAGCAACAATGCAGCAGGTGAAGAGAGCTCTGGTGATTTCTCTGTCTCCAGTTCTGGACAATGCGAAAACCGTCGGCCTCATCTCACTTCCTGGCGCAATGACTGGTCTCATAATGGGAGGAGCTTCTCCTTTGGAGGCCATTCATCTGCAGATTGTTGTGATGAACTTTCTGATTGGTGCATCTACAGTTAGCAGCATTTTTTCAACATATCTGTGTTGGCCTGCTTTCTTTACCAATGCTTATCAGTTAGAGACCAAGGTTTTCCATTCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0014

Protein Analysis

304

Amino Acids

32.8

Weight (kDa)

9.64

Isoelectric Point (pI)

22.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0014 PF03649 56 - 289 8.3e-68 Uncharacterised protein family (UPF0014)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37330
fragaria_vesca FvH4_6g14820 FvH4_6g14820
malus_domestica MD04G1110300.v1.1 MD12G1131100.v1.1
prunus_persica Prupe.6G245600_v2.0.a1
pyrus_communis pycom04g10580 pycom12g12690
rosa_chinensis RchiOBHm_Chr3g0468051
rosa_laevigata RLG00000024456
rosa_multiflora Rmu_sc0012651.1_g000001
rosa_roxburghii Rroxscaffold_6G00413150
rosa_rugosa Rorug03G0091900
rosa_samantha Rh3AG140400 Rh3BG161700 Rh3CG161700 Rh3DG162200
rosa_wichuraiana Rw3G013260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 640
Acc36I ACCTGC 1 cut(s) 640
AccII CGCG 1 cut(s) 631
AciI CCGC 6 cut(s) 24, 164, 170, 194, 512, 629
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 529
AcuI CTGAAG 2 cut(s) 278, 426
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 4 cut(s) 255, 349, 511, 512
AgsI TTSAA 2 cut(s) 534, 840
AjnI CCWGG 2 cut(s) 594, 724
AjuI GAANNNNNNNTTGG 4 cut(s) 75, 107, 209, 241
AluBI AGCT 3 cut(s) 271, 662, 758
AluI AGCT 3 cut(s) 271, 662, 758
Alw21I GWGCWC 2 cut(s) 189, 664
Alw26I GTCTC 4 cut(s) 596, 684, 746, 881
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 6 cut(s) 67, 198, 258, 709, 771, 854
ApeKI GCWGC 4 cut(s) 77, 466, 646, 828
ApoI RAATTY 1 cut(s) 529
AspLEI GCGC 2 cut(s) 633, 731
AspS9I GGNCC 1 cut(s) 258
AsuHPI GGTGA 4 cut(s) 169, 364, 664, 679
AsuII TTCGAA 1 cut(s) 580
AvaI CYCGRG 1 cut(s) 309
BanII GRGCYC 1 cut(s) 664
Bbv12I GWGCWC 2 cut(s) 189, 664
BbvI GCAGC 4 cut(s) 64, 453, 658, 840
BccI CCATC 2 cut(s) 127, 514
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 2 cut(s) 596, 726
BcoDI GTCTC 4 cut(s) 596, 684, 746, 881
BfaI CTAG 2 cut(s) 255, 399
BfmI CTRYAG 2 cut(s) 782, 819
BfuAI ACCTGC 1 cut(s) 640
BisI GCNGC 6 cut(s) 78, 164, 171, 467, 647, 829
BlpI GCTNAGC 2 cut(s) 387, 470
BlsI GCNGC 6 cut(s) 79, 165, 172, 468, 648, 830
Bme1390I CCNGG 2 cut(s) 596, 726
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 596, 726
BmrI ACTGGG 2 cut(s) 230, 555
BmsI GCATC 2 cut(s) 14, 824
BmuI ACTGGG 2 cut(s) 230, 555
BpmI CTGGAG 1 cut(s) 666
Bpu1102I GCTNAGC 2 cut(s) 387, 470
Bpu14I TTCGAA 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 131
BsaI GGTCTC 2 cut(s) 746, 881
BsaJI CCNNGG 1 cut(s) 891
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 349, 511, 512
Bse1I ACTGG 4 cut(s) 225, 550, 683, 742
Bse3DI GCAATG 3 cut(s) 42, 249, 738
BseBI CCWGG 2 cut(s) 596, 726
BseDI CCNNGG 1 cut(s) 891
BseGI GGATG 1 cut(s) 138
BseLI CCNNNNNNNGG 4 cut(s) 255, 349, 511, 512
BseMI GCAATG 3 cut(s) 42, 249, 738
BseMII CTCAG 2 cut(s) 93, 461
BseNI ACTGG 4 cut(s) 225, 550, 683, 742
BseRI GAGGAG 1 cut(s) 768
BseXI GCAGC 4 cut(s) 64, 453, 658, 840
BseYI CCCAGC 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 6 cut(s) 69, 200, 260, 711, 773, 856
BsiHKAI GWGCWC 2 cut(s) 189, 664
BsiHKCI CYCGRG 1 cut(s) 309
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 4 cut(s) 255, 349, 511, 512
BsmAI GTCTC 4 cut(s) 596, 684, 746, 881
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 6 cut(s) 69, 200, 260, 711, 773, 856
Bso31I GGTCTC 2 cut(s) 746, 881
BsoBI CYCGRG 1 cut(s) 309
Bsp119I TTCGAA 1 cut(s) 580
Bsp1286I GDGCHC 2 cut(s) 189, 664
Bsp143I GATC 1 cut(s) 327
Bsp1720I GCTNAGC 2 cut(s) 387, 470
BspACI CCGC 6 cut(s) 24, 164, 170, 194, 512, 629
BspANI GGCC 6 cut(s) 69, 200, 260, 711, 773, 856
BspCNI CTCAG 2 cut(s) 94, 462
BspFNI CGCG 1 cut(s) 631
BspMAI CTGCAG 1 cut(s) 786
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 1 cut(s) 335
BspT104I TTCGAA 1 cut(s) 580
BspTNI GGTCTC 2 cut(s) 746, 881
BsrDI GCAATG 3 cut(s) 42, 249, 738
BsrI ACTGG 4 cut(s) 225, 550, 683, 742
BssECI CCNNGG 1 cut(s) 891
BssMI GATC 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 596, 726
Bst4CI ACNGT 6 cut(s) 51, 178, 358, 541, 706, 823
Bst6I CTCTTC 1 cut(s) 650
BstBI TTCGAA 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 168, 858
BstDEI CTNAG 3 cut(s) 102, 387, 470
BstF5I GGATG 1 cut(s) 138
BstFNI CGCG 1 cut(s) 631
BstHHI GCGC 2 cut(s) 633, 731
BstKTI GATC 1 cut(s) 330
BstMAI GTCTC 4 cut(s) 596, 684, 746, 881
BstMBI GATC 1 cut(s) 327
BstMWI GCNNNNNNNGC 2 cut(s) 463, 620
BstNI CCWGG 2 cut(s) 596, 726
BstNSI RCATGY 2 cut(s) 214, 395
BstSCI CCNGG 2 cut(s) 594, 724
BstSFI CTRYAG 2 cut(s) 782, 819
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 4 cut(s) 64, 453, 658, 840
BsuRI GGCC 6 cut(s) 69, 200, 260, 711, 773, 856
BtsCI GGATG 1 cut(s) 138
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 2 cut(s) 168, 858
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 2 cut(s) 633, 731
Cfr13I GGNCC 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 1 cut(s) 208
CviAII CATG 7 cut(s) 118, 129, 154, 190, 211, 349, 392
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 3 cut(s) 102, 387, 470
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
Ecl136II GAGCTC 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 891
Eco147I AGGCCT 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 664
Eco31I GGTCTC 2 cut(s) 746, 881
Eco53kI GAGCTC 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 278, 426
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 662
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 2 cut(s) 594, 724
EcoT14I CCWWGG 1 cut(s) 891
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 7 cut(s) 121, 132, 157, 193, 214, 352, 395
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 326
FatI CATG 7 cut(s) 117, 128, 153, 189, 210, 348, 391
FauI CCCGC 1 cut(s) 505
Fnu4HI GCNGC 6 cut(s) 78, 164, 171, 467, 647, 829
FokI GGATG 1 cut(s) 145
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 6 cut(s) 78, 164, 171, 467, 647, 829
FspBI CTAG 2 cut(s) 255, 399
GlaI GCGC 2 cut(s) 632, 730
GluI GCNGC 6 cut(s) 78, 164, 171, 467, 647, 829
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 6 cut(s) 69, 200, 260, 711, 773, 856
HhaI GCGC 2 cut(s) 633, 731
Hin1II CATG 7 cut(s) 121, 132, 157, 193, 214, 352, 395
Hin6I GCGC 2 cut(s) 631, 729
HinP1I GCGC 2 cut(s) 631, 729
HphI GGTGA 4 cut(s) 169, 364, 664, 679
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 3 cut(s) 527, 807, 907
Hpy188III TCNNGA 4 cut(s) 274, 311, 569, 689
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 2 cut(s) 178, 710
HpyAV CCTTC 2 cut(s) 271, 493
HpyCH4III ACNGT 6 cut(s) 51, 178, 358, 541, 706, 823
HpyCH4V TGCA 6 cut(s) 47, 214, 623, 646, 784, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 620
HpyF3I CTNAG 3 cut(s) 102, 387, 470
Hsp92II CATG 7 cut(s) 121, 132, 157, 193, 214, 352, 395
HspAI GCGC 2 cut(s) 631, 729
Kzo9I GATC 1 cut(s) 327
LmnI GCTCC 2 cut(s) 15, 755
Lsp1109I GCAGC 4 cut(s) 64, 453, 658, 840
LweI GCATC 2 cut(s) 14, 824
MabI ACCWGGT 1 cut(s) 594
MaeI CTAG 2 cut(s) 255, 399
MaeIII GTNAC 4 cut(s) 352, 445, 541, 550
MalI GATC 1 cut(s) 329
MboI GATC 1 cut(s) 327
MboII GAAGA 4 cut(s) 83, 284, 295, 667
MhlI GDGCHC 2 cut(s) 189, 664
MluCI AATT 2 cut(s) 55, 529
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 5 cut(s) 23, 395, 722, 746, 763
MslI CAYNNNNRTG 2 cut(s) 194, 847
MspA1I CMGCKG 1 cut(s) 166
MspR9I CCNGG 2 cut(s) 596, 726
MvaI CCWGG 2 cut(s) 596, 726
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 2 cut(s) 463, 620
NdeII GATC 1 cut(s) 327
NlaIII CATG 7 cut(s) 121, 132, 157, 193, 214, 352, 395
NmuCI GTSAC 2 cut(s) 352, 445
NspI RCATGY 2 cut(s) 214, 395
NspV TTCGAA 1 cut(s) 580
PaqCI CACCTGC 1 cut(s) 640
PceI AGGCCT 1 cut(s) 69
PkrI GCNGC 6 cut(s) 79, 165, 172, 468, 648, 830
Psp124BI GAGCTC 1 cut(s) 664
Psp6I CCWGG 2 cut(s) 594, 724
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 2 cut(s) 594, 724
PspPI GGNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 220
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 2 cut(s) 194, 847
SacI GAGCTC 1 cut(s) 664
SatI GCNGC 6 cut(s) 78, 164, 171, 467, 647, 829
Sau3AI GATC 1 cut(s) 327
Sau96I GGNCC 1 cut(s) 258
ScrFI CCNGG 2 cut(s) 596, 726
SduI GDGCHC 2 cut(s) 189, 664
SetI ASST 8 cut(s) 103, 273, 345, 597, 654, 664, 760, 897
SexAI ACCWGGT 1 cut(s) 594
SfaNI GCATC 2 cut(s) 14, 824
SfcI CTRYAG 2 cut(s) 782, 819
SfuI TTCGAA 1 cut(s) 580
SmiMI CAYNNNNRTG 2 cut(s) 194, 847
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 2 cut(s) 55, 529
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 6 cut(s) 24, 164, 170, 194, 512, 629
SspMI CTAG 2 cut(s) 255, 399
SstI GAGCTC 1 cut(s) 664
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 594, 724
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 6 cut(s) 51, 178, 358, 541, 706, 823
TaqI TCGA 3 cut(s) 275, 568, 580
TasI AATT 2 cut(s) 55, 529
TauI GCSGC 2 cut(s) 166, 173
TseFI GTSAC 2 cut(s) 352, 445
TseI GCWGC 4 cut(s) 77, 466, 646, 828
Tsp45I GTSAC 2 cut(s) 352, 445
TspDTI ATGAA 8 cut(s) 49, 253, 289, 337, 572, 605, 767, 812
XapI RAATTY 1 cut(s) 529
XceI RCATGY 2 cut(s) 214, 395
XspI CTAG 2 cut(s) 255, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.