MD12G1131100.v1.1

Protein ALUMINUM SENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013110
Physical Location & Seq
Forward (+)
20642798 .. 20645178
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1131100.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCGTCACTCATCTCCGCCACCGCCCAAAACCACACAGACATCGCACAGTCCAATTTCATACAAGGCCTATTTGCCGACCAGTTTCTTGTTTCCTTGCCTGAATTTCTGGCCAACATGGACTTGTCATGGATGGTGGACTTTCTCAAGGGCATGGTGAAGCCGCTGGCGGCGACGGTGGTGGTGTTCCTTGCGGTGGCCTTGTCATACATGCAGAAGCTGGGTTTGGGTAGAGAGATGATTTACTCCATTTTTAGAGCCTTCGTTCAGCTTTCGATTATCGGGTTTGTTCTTCAGTTCATCTTCACTCGGGACAATGCCTTTTGGATCGTTCTAGCTTACCTTTTCATGGTGACTGTTGCTGGTTATACAGCAGGGCAAAGGGCTAAGCATGTCCCAAGAGGGAAGTATGTTGCTGGGGCTTCCATATTGGCTGGAACTTCAATCACAATGTTTCTGCTTCTTGTGATGAGAGTGTTCCCATTCACTCCAAGATATATTATCCCCGTTGCAGGAATGATGGTCGGAAATTCAATGACGGTAACTGGGGTTACGATGAAAAGGCTTCGAGATGATATTCGAACTCAAATGAACCTGGTAGAGACAGCGTTGGCTCTTGGTGCAACGCCGCGCCAAGCAACAATGCAGCAGGTGAAGAGAGCTCTGGTGATTTCTCTGTCTCCAGTTCTGGACAATGCCAAAACCGTTGGCCTCATCTCGCTTCCCGGTGCAATGACTGGCCTCATAATGGGAGGAGCCTCCCCTCTGGAGGCCATTCAATTGCAGATTGTTGTGATGAACTTTATGATCGGTGCAACTACAGTTAGCAGCATTTCTTCAACGTATCTGTGTTGGCCTGCCTTCTTTACCAAAGCTTATCAGTTAGAGACCAAGGTTTTCACTTTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0014

Protein Analysis

304

Amino Acids

33.16

Weight (kDa)

9.71

Isoelectric Point (pI)

19.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0014 PF03649 56 - 289 8.1e-68 Uncharacterised protein family (UPF0014)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37330
fragaria_vesca FvH4_6g14820 FvH4_6g14820
malus_domestica MD04G1110300.v1.1 MD12G1131100.v1.1
prunus_persica Prupe.6G245600_v2.0.a1
pyrus_communis pycom04g10580 pycom12g12690
rosa_chinensis RchiOBHm_Chr3g0468051
rosa_laevigata RLG00000024456
rosa_multiflora Rmu_sc0012651.1_g000001
rosa_roxburghii Rroxscaffold_6G00413150
rosa_rugosa Rorug03G0091900
rosa_samantha Rh3AG140400 Rh3BG161700 Rh3CG161700 Rh3DG162200
rosa_wichuraiana Rw3G013260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 640
Acc36I ACCTGC 1 cut(s) 640
AccII CGCG 1 cut(s) 631
AciI CCGC 6 cut(s) 18, 24, 164, 170, 194, 629
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 2 cut(s) 104, 529
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 5 cut(s) 196, 349, 512, 748, 769
AgsI TTSAA 4 cut(s) 444, 534, 779, 840
AjnI CCWGG 1 cut(s) 594
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 5 cut(s) 220, 271, 338, 662, 875
AluI AGCT 5 cut(s) 220, 271, 338, 662, 875
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 3 cut(s) 596, 684, 881
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 7 cut(s) 67, 111, 198, 709, 739, 771, 854
ApeKI GCWGC 2 cut(s) 646, 828
ApoI RAATTY 2 cut(s) 104, 529
AspLEI GCGC 1 cut(s) 633
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 4 cut(s) 169, 364, 664, 679
AsuII TTCGAA 1 cut(s) 580
AvaI CYCGRG 1 cut(s) 309
BalI TGGCCA 1 cut(s) 113
BanII GRGCYC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 2 cut(s) 658, 840
BccI CCATC 2 cut(s) 127, 514
BciT130I CCWGG 1 cut(s) 596
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 3 cut(s) 596, 684, 881
BfaI CTAG 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 640
BisI GCNGC 5 cut(s) 164, 171, 629, 647, 829
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 5 cut(s) 165, 172, 630, 648, 830
Bme1390I CCNGG 2 cut(s) 596, 726
BmeT110I CYCGRG 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 757
BmrFI CCNGG 2 cut(s) 596, 726
BmrI ACTGGG 1 cut(s) 555
BmuI ACTGGG 1 cut(s) 555
BpmI CTGGAG 2 cut(s) 666, 788
Bpu1102I GCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 881
BsaJI CCNNGG 1 cut(s) 891
Bsc4I CCNNNNNNNGG 5 cut(s) 196, 349, 512, 748, 769
Bse1I ACTGG 4 cut(s) 82, 550, 683, 742
Bse3DI GCAATG 1 cut(s) 738
BseBI CCWGG 1 cut(s) 596
BseDI CCNNGG 1 cut(s) 891
BseGI GGATG 1 cut(s) 138
BseLI CCNNNNNNNGG 5 cut(s) 196, 349, 512, 748, 769
BseMI GCAATG 1 cut(s) 738
BseNI ACTGG 4 cut(s) 82, 550, 683, 742
BseRI GAGGAG 1 cut(s) 768
BseXI GCAGC 2 cut(s) 658, 840
BseYI CCCAGC 2 cut(s) 220, 416
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 7 cut(s) 69, 113, 200, 711, 741, 773, 856
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 1 cut(s) 726
BslFI GGGAC 2 cut(s) 326, 380
BslI CCNNNNNNNGG 5 cut(s) 196, 349, 512, 748, 769
BsmAI GTCTC 3 cut(s) 596, 684, 881
BsmFI GGGAC 2 cut(s) 326, 380
BsnI GGCC 7 cut(s) 69, 113, 200, 711, 741, 773, 856
Bso31I GGTCTC 1 cut(s) 881
BsoBI CYCGRG 1 cut(s) 309
Bsp119I TTCGAA 1 cut(s) 580
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 2 cut(s) 327, 807
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 6 cut(s) 18, 24, 164, 170, 194, 629
BspANI GGCC 7 cut(s) 69, 113, 200, 711, 741, 773, 856
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 757
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 1 cut(s) 335
BspT104I TTCGAA 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 881
BsrDI GCAATG 1 cut(s) 738
BsrI ACTGG 4 cut(s) 82, 550, 683, 742
BssECI CCNNGG 1 cut(s) 891
BssMI GATC 2 cut(s) 327, 807
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 891
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 6 cut(s) 51, 178, 358, 541, 706, 823
Bst6I CTCTTC 1 cut(s) 650
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 168, 858
BstDEI CTNAG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 138
BstFNI CGCG 1 cut(s) 631
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 2 cut(s) 330, 810
BstMAI GTCTC 3 cut(s) 596, 684, 881
BstMBI GATC 2 cut(s) 327, 807
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 2 cut(s) 214, 395
BstSCI CCNGG 2 cut(s) 594, 724
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 2 cut(s) 658, 840
BsuRI GGCC 7 cut(s) 69, 113, 200, 711, 741, 773, 856
BtgZI GCGATG 1 cut(s) 28
BtsCI GGATG 1 cut(s) 138
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 2 cut(s) 168, 858
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 1 cut(s) 633
CsiI ACCWGGT 1 cut(s) 594
CviAII CATG 6 cut(s) 118, 129, 154, 211, 349, 392
DdeI CTNAG 1 cut(s) 387
DpnI GATC 2 cut(s) 329, 809
DpnII GATC 2 cut(s) 327, 807
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 650
EarI CTCTTC 1 cut(s) 650
EciI GGCGGA 1 cut(s) 7
Ecl136II GAGCTC 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 891
Eco147I AGGCCT 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 664
Eco31I GGTCTC 1 cut(s) 881
Eco53kI GAGCTC 1 cut(s) 662
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 594
EcoT14I CCWWGG 1 cut(s) 891
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 6 cut(s) 121, 132, 157, 214, 352, 395
FaqI GGGAC 2 cut(s) 326, 380
FatI CATG 6 cut(s) 117, 128, 153, 210, 348, 391
Fnu4HI GCNGC 5 cut(s) 164, 171, 629, 647, 829
FokI GGATG 1 cut(s) 145
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 5 cut(s) 164, 171, 629, 647, 829
FspBI CTAG 1 cut(s) 335
GlaI GCGC 1 cut(s) 632
GluI GCNGC 5 cut(s) 164, 171, 629, 647, 829
GsaI CCCAGC 2 cut(s) 224, 420
GsuI CTGGAG 2 cut(s) 666, 788
HaeIII GGCC 7 cut(s) 69, 113, 200, 711, 741, 773, 856
HapII CCGG 1 cut(s) 726
HhaI GCGC 1 cut(s) 633
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 121, 132, 157, 214, 352, 395
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 873
HpaII CCGG 1 cut(s) 726
HphI GGTGA 4 cut(s) 169, 364, 664, 679
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 527
Hpy188III TCNNGA 4 cut(s) 311, 569, 689, 767
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 271, 871
HpyCH4III ACNGT 6 cut(s) 51, 178, 358, 541, 706, 823
HpyCH4IV ACGT 1 cut(s) 842
HpyCH4V TGCA 7 cut(s) 214, 512, 623, 646, 731, 784, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 1 cut(s) 842
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 121, 132, 157, 214, 352, 395
HspAI GCGC 1 cut(s) 631
Kzo9I GATC 2 cut(s) 327, 807
LmnI GCTCC 1 cut(s) 755
Lsp1109I GCAGC 2 cut(s) 658, 840
MabI ACCWGGT 1 cut(s) 594
MaeI CTAG 1 cut(s) 335
MaeII ACGT 1 cut(s) 842
MaeIII GTNAC 4 cut(s) 6, 352, 541, 550
MalI GATC 2 cut(s) 329, 809
MboI GATC 2 cut(s) 327, 807
MboII GAAGA 4 cut(s) 284, 295, 667, 828
MfeI CAATTG 1 cut(s) 779
MhlI GDGCHC 1 cut(s) 664
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 4 cut(s) 55, 104, 529, 779
MluNI TGGCCA 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 7 cut(s) 395, 722, 746, 752, 763, 769, 774
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 113
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 2 cut(s) 596, 726
MunI CAATTG 1 cut(s) 779
MvaI CCWGG 1 cut(s) 596
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 1 cut(s) 620
NciI CCSGG 1 cut(s) 726
NdeII GATC 2 cut(s) 327, 807
NlaIII CATG 6 cut(s) 121, 132, 157, 214, 352, 395
NlaIV GGNNCC 1 cut(s) 757
NmuCI GTSAC 2 cut(s) 6, 352
NspI RCATGY 2 cut(s) 214, 395
NspV TTCGAA 1 cut(s) 580
PaqCI CACCTGC 1 cut(s) 640
PceI AGGCCT 1 cut(s) 69
PkrI GCNGC 5 cut(s) 165, 172, 630, 648, 830
Psp124BI GAGCTC 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 594
PspFI CCCAGC 2 cut(s) 220, 416
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 757
PstNI CAGNNNCTG 1 cut(s) 220
SacI GAGCTC 1 cut(s) 664
SatI GCNGC 5 cut(s) 164, 171, 629, 647, 829
Sau3AI GATC 2 cut(s) 327, 807
ScrFI CCNGG 2 cut(s) 596, 726
SduI GDGCHC 1 cut(s) 664
SexAI ACCWGGT 1 cut(s) 594
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 580
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 4 cut(s) 55, 104, 529, 779
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 6 cut(s) 18, 24, 164, 170, 194, 629
SspMI CTAG 1 cut(s) 335
SstI GAGCTC 1 cut(s) 664
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 594, 724
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 6 cut(s) 51, 178, 358, 541, 706, 823
TaiI ACGT 1 cut(s) 845
TaqI TCGA 3 cut(s) 275, 568, 580
TasI AATT 4 cut(s) 55, 104, 529, 779
TauI GCSGC 3 cut(s) 166, 173, 631
TseFI GTSAC 2 cut(s) 6, 352
TseI GCWGC 2 cut(s) 646, 828
Tsp45I GTSAC 2 cut(s) 6, 352
TspDTI ATGAA 6 cut(s) 49, 289, 337, 572, 605, 812
XapI RAATTY 2 cut(s) 104, 529
XceI RCATGY 2 cut(s) 214, 395
XspI CTAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.