pycom12g12690

Protein ALUMINUM SENSITIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013110
Physical Location & Seq
Forward (+)
15149197 .. 15151253
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12690.2

Sequence Viewer

Length: 1074 bp
ATGCGAAAATATTCTCTTAAAAAATCTTGGAGTTGTCAACTCCACCACATCATTCGAACTTCAGGGGGAGGTCGTTTCCTCACTCTATCTCTCTCCCTCTCTCCAATCCACATATTACTAATTTCTTCACGCAATATCCGAATTTCGTATCAACTATCACAAATGGCGTCACTCATCTCCGCCACCGCGCAAAACCACACAGACATCGCACAGTCCAATTTCATACAAGGCCTATTTGCCGACCAGGTTCTTGTTTCCTTGCCTGAATTTCTGGCCAACATGGACTTCTCATGGATGGTGGACTTTCTCAAGGGCATGGTGAAGCCGCTGGCGGCGACGGTGGTGGTGTTCCTGGCGGTTGCCTTGTCATACGTGGAGAAGCTGGGTTTGGGTAGAGAGATGATTTACTCCATTTGTAGAGCCTTCGTTCAGCTTTCGATTATCGGGTTTGTTCTTCAGTTCATCTTCACTCGGGACAATGCCGTTTGGATCGTTCTGGCTTACCTTTTCATGGTGACTGTTGCTGGTTATACAGCAGGGCAAAGGGCTAAGCATGTCCCAAGAGGGAAGTATGTTGCTGGGGCTTCCATATTGGCTGGAACTTCAATCACAATGTTTCTGCTTCTTGTGATGAGAGTGTTCCCCTTCAGTCCAAGATATATTATCCCCGTTGCAGGAATGATGGTCGGAAATTCAATGACGGTAACTGGGGTTACGATGAAGAGGCTTCGAGATGATATTCGAACTCAAATGAACCTGGTGGAGACAGCGTTGGCTCTTGGCGCAACGCCGCGCCAAGCAACAATGCAGCAGGTGAAGAGAGCTCTGGTGATTTCTCTGTCTCCGGTTCTGGACAATGCCAAAACCGTCGGCCTCATCTCGCTTCCCGGTGCAATGACTGGCCTCATAATGGGAGGAGCCTCCCCTCTGGAGGCCATTCAATTGCAGATTGTTGTGATGAACTTTATGATTGGTGCAACTACAGTTAGCAGCATTTCTTCAACATATCTGTGTTGGCCTGCCTTCTTTACCAAAGCTTATCAGTTAGAGACCAAGGTTTTCACTTCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0014

Protein Analysis

358

Amino Acids

39.19

Weight (kDa)

9.97

Isoelectric Point (pI)

27.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37330
fragaria_vesca FvH4_6g14820 FvH4_6g14820
malus_domestica MD04G1110300.v1.1 MD12G1131100.v1.1
prunus_persica Prupe.6G245600_v2.0.a1
pyrus_communis pycom04g10580 pycom12g12690
rosa_chinensis RchiOBHm_Chr3g0468051
rosa_laevigata RLG00000024456
rosa_multiflora Rmu_sc0012651.1_g000001
rosa_roxburghii Rroxscaffold_6G00413150
rosa_rugosa Rorug03G0091900
rosa_samantha Rh3AG140400 Rh3BG161700 Rh3CG161700 Rh3DG162200
rosa_wichuraiana Rw3G013260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 802
Acc36I ACCTGC 1 cut(s) 802
AccII CGCG 2 cut(s) 188, 793
AciI CCGC 6 cut(s) 180, 186, 326, 332, 356, 791
AclWI GGATC 1 cut(s) 497
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 3 cut(s) 141, 266, 691
AcuI CTGAAG 4 cut(s) 45, 440, 631, 1050
AcyI GRCGYC 1 cut(s) 167
AfiI CCNNNNNNNGG 4 cut(s) 511, 674, 910, 931
AgsI TTSAA 4 cut(s) 606, 696, 941, 1002
AjnI CCWGG 3 cut(s) 243, 351, 756
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 371, 403
AluBI AGCT 4 cut(s) 382, 433, 824, 1037
AluI AGCT 4 cut(s) 382, 433, 824, 1037
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 3 cut(s) 758, 846, 1043
AlwI GGATC 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 471
AoxI GGCC 6 cut(s) 229, 273, 871, 901, 933, 1016
ApeKI GCWGC 2 cut(s) 808, 990
ApoI RAATTY 3 cut(s) 141, 266, 691
Asp700I GAANNNNTTC 1 cut(s) 10
AspLEI GCGC 3 cut(s) 190, 785, 795
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 4 cut(s) 331, 526, 826, 841
AsuII TTCGAA 2 cut(s) 55, 742
AvaI CYCGRG 1 cut(s) 471
BalI TGGCCA 1 cut(s) 275
BanII GRGCYC 1 cut(s) 826
Bbv12I GWGCWC 1 cut(s) 826
BbvI GCAGC 2 cut(s) 820, 1002
BccI CCATC 2 cut(s) 289, 676
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 3 cut(s) 245, 353, 758
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 3 cut(s) 758, 846, 1043
BfmI CTRYAG 1 cut(s) 981
BfuAI ACCTGC 1 cut(s) 802
BisI GCNGC 5 cut(s) 326, 333, 791, 809, 991
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 327, 334, 792, 810, 992
Bme1390I CCNGG 4 cut(s) 245, 353, 758, 888
BmeT110I CYCGRG 1 cut(s) 471
BmiI GGNNCC 1 cut(s) 919
BmrFI CCNGG 4 cut(s) 245, 353, 758, 888
BmrI ACTGGG 1 cut(s) 717
BmuI ACTGGG 1 cut(s) 717
BpmI CTGGAG 1 cut(s) 950
Bpu1102I GCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 2 cut(s) 55, 742
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 888
BsaAI YACGTR 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 1043
BsaJI CCNNGG 1 cut(s) 1053
BsaWI WCCGGW 1 cut(s) 844
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 674, 910, 931
Bse1I ACTGG 2 cut(s) 712, 904
Bse3DI GCAATG 1 cut(s) 900
BseBI CCWGG 3 cut(s) 245, 353, 758
BseDI CCNNGG 1 cut(s) 1053
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 4 cut(s) 511, 674, 910, 931
BseMI GCAATG 1 cut(s) 900
BseNI ACTGG 2 cut(s) 712, 904
BseRI GAGGAG 1 cut(s) 930
BseXI GCAGC 2 cut(s) 820, 1002
BseYI CCCAGC 2 cut(s) 382, 578
Bsh1236I CGCG 2 cut(s) 188, 793
BshFI GGCC 6 cut(s) 231, 275, 873, 903, 935, 1018
BsiHKAI GWGCWC 1 cut(s) 826
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 2 cut(s) 845, 888
BslFI GGGAC 2 cut(s) 488, 542
BslI CCNNNNNNNGG 4 cut(s) 511, 674, 910, 931
BsmAI GTCTC 3 cut(s) 758, 846, 1043
BsmFI GGGAC 2 cut(s) 488, 542
BsnI GGCC 6 cut(s) 231, 275, 873, 903, 935, 1018
Bso31I GGTCTC 1 cut(s) 1043
BsoBI CYCGRG 1 cut(s) 471
Bsp119I TTCGAA 2 cut(s) 55, 742
Bsp1286I GDGCHC 1 cut(s) 826
Bsp143I GATC 1 cut(s) 489
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 6 cut(s) 180, 186, 326, 332, 356, 791
BspANI GGCC 6 cut(s) 231, 275, 873, 903, 935, 1018
BspFNI CGCG 2 cut(s) 188, 793
BspLI GGNNCC 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 1 cut(s) 497
BspT104I TTCGAA 2 cut(s) 55, 742
BspTNI GGTCTC 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 900
BsrI ACTGG 2 cut(s) 712, 904
BssECI CCNNGG 1 cut(s) 1053
BssMI GATC 1 cut(s) 489
BssNI GRCGYC 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 1053
Bst2UI CCWGG 3 cut(s) 245, 353, 758
Bst4CI ACNGT 6 cut(s) 213, 340, 520, 703, 868, 985
Bst6I CTCTTC 2 cut(s) 716, 812
BstACI GRCGYC 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 373
BstBI TTCGAA 2 cut(s) 55, 742
BstC8I GCNNGC 2 cut(s) 330, 1020
BstDEI CTNAG 1 cut(s) 549
BstF5I GGATG 1 cut(s) 300
BstFNI CGCG 2 cut(s) 188, 793
BstHHI GCGC 3 cut(s) 190, 785, 795
BstKTI GATC 1 cut(s) 492
BstMAI GTCTC 3 cut(s) 758, 846, 1043
BstMBI GATC 1 cut(s) 489
BstMWI GCNNNNNNNGC 1 cut(s) 782
BstNI CCWGG 3 cut(s) 245, 353, 758
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 4 cut(s) 243, 351, 756, 886
BstSFI CTRYAG 1 cut(s) 981
BstUI CGCG 2 cut(s) 188, 793
BstV1I GCAGC 2 cut(s) 820, 1002
BsuRI GGCC 6 cut(s) 231, 275, 873, 903, 935, 1018
BtgZI GCGATG 1 cut(s) 190
BtsCI GGATG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 330, 1020
CfoI GCGC 3 cut(s) 190, 785, 795
CseI GACGC 1 cut(s) 156
CsiI ACCWGGT 2 cut(s) 243, 756
CviAII CATG 5 cut(s) 280, 291, 316, 511, 554
DdeI CTNAG 1 cut(s) 549
DpnI GATC 1 cut(s) 491
DpnII GATC 1 cut(s) 489
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 716, 812
EarI CTCTTC 2 cut(s) 716, 812
EciI GGCGGA 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 1053
Eco147I AGGCCT 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 826
Eco31I GGTCTC 1 cut(s) 1043
Eco53kI GAGCTC 1 cut(s) 824
Eco57I CTGAAG 4 cut(s) 45, 440, 631, 1050
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 824
EcoRII CCWGG 3 cut(s) 243, 351, 756
EcoT14I CCWWGG 1 cut(s) 1053
EcoT38I GRGCYC 1 cut(s) 826
ErhI CCWWGG 1 cut(s) 1053
FaeI CATG 5 cut(s) 283, 294, 319, 514, 557
FaqI GGGAC 2 cut(s) 488, 542
FatI CATG 5 cut(s) 279, 290, 315, 510, 553
Fnu4HI GCNGC 5 cut(s) 326, 333, 791, 809, 991
FokI GGATG 1 cut(s) 307
FriOI GRGCYC 1 cut(s) 826
Fsp4HI GCNGC 5 cut(s) 326, 333, 791, 809, 991
GlaI GCGC 3 cut(s) 189, 784, 794
GluI GCNGC 5 cut(s) 326, 333, 791, 809, 991
GsaI CCCAGC 2 cut(s) 386, 582
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 6 cut(s) 231, 275, 873, 903, 935, 1018
HapII CCGG 2 cut(s) 845, 888
HgaI GACGC 1 cut(s) 156
HhaI GCGC 3 cut(s) 190, 785, 795
Hin1I GRCGYC 1 cut(s) 167
Hin1II CATG 5 cut(s) 283, 294, 319, 514, 557
Hin6I GCGC 3 cut(s) 188, 783, 793
HinP1I GCGC 3 cut(s) 188, 783, 793
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 1035
HpaII CCGG 2 cut(s) 845, 888
HphI GGTGA 4 cut(s) 331, 526, 826, 841
Hpy166II GTNNAC 2 cut(s) 38, 301
Hpy188I TCNGA 3 cut(s) 140, 689, 1069
Hpy188III TCNNGA 4 cut(s) 473, 731, 851, 929
Hpy8I GTNNAC 2 cut(s) 38, 301
Hpy99I CGWCG 2 cut(s) 340, 872
HpyAV CCTTC 3 cut(s) 433, 655, 1033
HpyCH4III ACNGT 6 cut(s) 213, 340, 520, 703, 868, 985
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 5 cut(s) 674, 808, 893, 946, 977
HpyF10VI GCNNNNNNNGC 1 cut(s) 782
HpyF3I CTNAG 1 cut(s) 549
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 167
Hsp92II CATG 5 cut(s) 283, 294, 319, 514, 557
HspAI GCGC 3 cut(s) 188, 783, 793
Kzo9I GATC 1 cut(s) 489
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 2 cut(s) 820, 1002
MabI ACCWGGT 2 cut(s) 243, 756
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 4 cut(s) 168, 514, 703, 712
MalI GATC 1 cut(s) 491
MboI GATC 1 cut(s) 489
MboII GAAGA 6 cut(s) 117, 446, 457, 733, 829, 990
MfeI CAATTG 1 cut(s) 941
MhlI GDGCHC 1 cut(s) 826
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 6 cut(s) 120, 141, 217, 266, 691, 941
MluNI TGGCCA 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 667
Mox20I TGGCCA 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 10
MscI TGGCCA 1 cut(s) 275
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 1 cut(s) 1009
Msp20I TGGCCA 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 328
MspI CCGG 2 cut(s) 845, 888
MspR9I CCNGG 4 cut(s) 245, 353, 758, 888
MunI CAATTG 1 cut(s) 941
MvaI CCWGG 3 cut(s) 245, 353, 758
MvnI CGCG 2 cut(s) 188, 793
MwoI GCNNNNNNNGC 1 cut(s) 782
NciI CCSGG 1 cut(s) 888
NdeII GATC 1 cut(s) 489
NlaIII CATG 5 cut(s) 283, 294, 319, 514, 557
NlaIV GGNNCC 1 cut(s) 919
NmuCI GTSAC 2 cut(s) 168, 514
NspI RCATGY 1 cut(s) 557
NspV TTCGAA 2 cut(s) 55, 742
PaqCI CACCTGC 1 cut(s) 802
PceI AGGCCT 1 cut(s) 231
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 10
PkrI GCNGC 5 cut(s) 327, 334, 792, 810, 992
Ppu21I YACGTR 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 826
Psp6I CCWGG 3 cut(s) 243, 351, 756
PspFI CCCAGC 2 cut(s) 382, 578
PspGI CCWGG 3 cut(s) 243, 351, 756
PspN4I GGNNCC 1 cut(s) 919
RseI CAYNNNNRTG 1 cut(s) 1009
SacI GAGCTC 1 cut(s) 826
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 5 cut(s) 326, 333, 791, 809, 991
Sau3AI GATC 1 cut(s) 489
ScrFI CCNGG 4 cut(s) 245, 353, 758, 888
SduI GDGCHC 1 cut(s) 826
SexAI ACCWGGT 2 cut(s) 243, 756
SfcI CTRYAG 1 cut(s) 981
SfuI TTCGAA 2 cut(s) 55, 742
SmiMI CAYNNNNRTG 1 cut(s) 1009
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 6 cut(s) 120, 141, 217, 266, 691, 941
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 6 cut(s) 180, 186, 326, 332, 356, 791
SspI AATATT 1 cut(s) 11
SstI GAGCTC 1 cut(s) 826
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 4 cut(s) 243, 351, 756, 886
StyI CCWWGG 1 cut(s) 1053
TaaI ACNGT 6 cut(s) 213, 340, 520, 703, 868, 985
TaiI ACGT 1 cut(s) 375
TaqI TCGA 4 cut(s) 55, 437, 730, 742
TasI AATT 6 cut(s) 120, 141, 217, 266, 691, 941
TauI GCSGC 3 cut(s) 328, 335, 793
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TseFI GTSAC 2 cut(s) 168, 514
TseI GCWGC 2 cut(s) 808, 990
Tsp45I GTSAC 2 cut(s) 168, 514
TspDTI ATGAA 6 cut(s) 211, 451, 499, 734, 767, 974
XapI RAATTY 3 cut(s) 141, 266, 691
XceI RCATGY 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.