MD04G1110400.v1.1

Ribosomal protein S24e family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012360
Physical Location & Seq
Forward (+)
19506135 .. 19508458
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1110400.v1.1.491

Sequence Viewer

Length: 918 bp
ATGAAAGAAAAGCAACAGCACTACATAAATGGGTCAGGGGCAGAATCTATAATTTCGCTACAGCTCCGGGGCATTTCTCGAATTCTACTTTCTCGAAACGACAATCACCGTCCTCACCCCTTAGTACTCAGTAGAGCAGAGACACTAAAGCCGCGCCGCCGCCGTGAATCTCCAAAAGCTCCGGAAAAAGCAAAAGAGAGAGCAAAAATGTCGAAGCTCGGTCTTCTCCGCGCCGCAATCACGTCAAGCATCGCCGTACGTACGGCCGTGCCGCAGAACCGCGGCCTCCCTATGCTCCTCGGCGAACCGGCGAGACGGTTCTCGACAGAAGCCGAAAACCCGCCTCAGAACTCCCGGCCGTCTGATCAGCCGCAGGATGCTTCACCGATCGAACCATTTCTCCAAACCCCAACCACAGGTTTGGTGTATGGGAAGCTGCTTGGAATCTCGAGGTATACGCTGAAGACCGATGTGGTGAATATGCTGGAGGGCTGTAATTTGAGCCTGGAGGATGTCAAAGTTGATTACAATCGGGCCTTCACGCCGATGGGAATGGTCGTGCAATTCCGGTCATGGAAAGCTTTTGATAATGCAGCTAGGGTGATTTCCAGGAAGGGCCGCCTGTTTAAATTGGAGAGGGCTAACCGATCGCAGTGGGACAATCTTACTCCCCATGATGCAAAAACTGTTTTATTGCAAGGAATTCCTCAAACTGCGGCTCCAGAAGATGTAGAACGGTTTTTATCTGGTTGTGAATACGATTCATCTTCCCTCCAGATAATTTTCAGGTCTTTCCCAGAACCCTCCAAATTTGCAACTGTTCGCTTTCCGTCTCAAACCGAGGCAATGAATGCATTCCTTGCGAAGAACAAAGGCTACTGTCAGAATGGTCAAATTTTGATGCGCGTTCTCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

34.32

Weight (kDa)

10.07

Isoelectric Point (pI)

59.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02740 AT5G02740
fragaria_vesca FvH4_6g14830
malus_domestica MD04G1110400.v1.1
prunus_persica Prupe.6G245500_v2.0.a1
pyrus_communis pycom04g10570 pycom13g21250
rosa_chinensis RchiOBHm_Chr3g0468061
rosa_laevigata RLG00000024455
rosa_multiflora Rmu_sc0005326.1_g000003 Rmu_sc0012651.1_g000003
rosa_roxburghii Rroxscaffold_6G00413110
rosa_rugosa Rorug03G0091900 Rorug03G0092000
rosa_samantha Rh3AG140500 Rh3BG161800 Rh3CG161800 Rh3DG162300
rosa_wichuraiana Rw3G013270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 455
AccII CGCG 4 cut(s) 154, 231, 282, 906
AccIII TCCGGA 1 cut(s) 181
AcoI YGGCCR 2 cut(s) 264, 356
AcsI RAATTY 4 cut(s) 81, 702, 809, 894
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 3 cut(s) 126, 258, 262
AfiI CCNNNNNNNGG 1 cut(s) 416
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 2 cut(s) 504, 608
AloI GAACNNNNNNTCC 2 cut(s) 384, 416
AluBI AGCT 6 cut(s) 64, 179, 217, 436, 581, 596
AluI AGCT 6 cut(s) 64, 179, 217, 436, 581, 596
Alw26I GTCTC 3 cut(s) 134, 307, 837
Ama87I CYCGRG 1 cut(s) 448
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 5 cut(s) 264, 283, 356, 534, 616
ApeKI GCWGC 2 cut(s) 436, 593
ApoI RAATTY 4 cut(s) 81, 702, 809, 894
Asp700I GAANNNNTTC 2 cut(s) 396, 854
AspLEI GCGC 3 cut(s) 156, 233, 906
AspS9I GGNCC 2 cut(s) 534, 616
AsuC2I CCSGG 2 cut(s) 68, 355
AsuHPI GGTGA 5 cut(s) 98, 107, 375, 487, 613
AvaI CYCGRG 1 cut(s) 448
BbsI GAAGAC 2 cut(s) 215, 470
BbvI GCAGC 2 cut(s) 423, 605
BccI CCATC 1 cut(s) 541
BceAI ACGGC 5 cut(s) 147, 239, 251, 279, 343
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BciT130I CCWGG 2 cut(s) 506, 610
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 2 cut(s) 68, 355
BcoDI GTCTC 3 cut(s) 134, 307, 837
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 59
BmcAI AGTACT 1 cut(s) 126
Bme1390I CCNGG 4 cut(s) 68, 355, 506, 610
BmeT110I CYCGRG 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 243
BmgT120I GGNCC 2 cut(s) 534, 616
BmiI GGNNCC 1 cut(s) 720
BmrFI CCNGG 4 cut(s) 68, 355, 506, 610
BmsI GCATC 4 cut(s) 258, 367, 667, 891
BpiI GAAGAC 2 cut(s) 215, 470
BpmI CTGGAG 5 cut(s) 506, 527, 705, 758, 896
BpuMI CCSGG 2 cut(s) 68, 355
BsaAI YACGTR 1 cut(s) 260
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 4 cut(s) 67, 280, 298, 840
BsaWI WCCGGW 2 cut(s) 181, 567
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 703, 733
Bsc4I CCNNNNNNNGG 1 cut(s) 416
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 913
Bse3DI GCAATG 1 cut(s) 852
Bse8I GATNNNNATC 1 cut(s) 528
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 2 cut(s) 506, 610
BseDI CCNNGG 4 cut(s) 67, 280, 298, 840
BseGI GGATG 2 cut(s) 382, 517
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 142, 359
BseNI ACTGG 1 cut(s) 913
BseRI GAGGAG 1 cut(s) 287
BseX3I CGGCCG 2 cut(s) 264, 356
BseXI GCAGC 2 cut(s) 423, 605
Bsh1236I CGCG 4 cut(s) 154, 231, 282, 906
Bsh1285I CGRYCG 4 cut(s) 267, 359, 390, 650
BshFI GGCC 5 cut(s) 266, 285, 358, 536, 618
BsiEI CGRYCG 4 cut(s) 267, 359, 390, 650
BsiHKCI CYCGRG 1 cut(s) 448
BsiSI CCGG 5 cut(s) 67, 182, 308, 355, 568
BsiWI CGTACG 2 cut(s) 256, 260
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 1 cut(s) 416
BsmAI GTCTC 3 cut(s) 134, 307, 837
BsmBI CGTCTC 2 cut(s) 307, 837
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 2 cut(s) 854, 856
BsnI GGCC 5 cut(s) 266, 285, 358, 536, 618
BsoBI CYCGRG 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 3 cut(s) 364, 387, 647
BspANI GGCC 5 cut(s) 266, 285, 358, 536, 618
BspCNI CTCAG 2 cut(s) 141, 358
BspEI TCCGGA 1 cut(s) 181
BspFNI CGCG 4 cut(s) 154, 231, 282, 906
BspLI GGNNCC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 913
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 4 cut(s) 67, 280, 298, 840
BssMI GATC 3 cut(s) 364, 387, 647
BssNAI GTATAC 1 cut(s) 456
Bst1107I GTATAC 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 506, 610
Bst4CI ACNGT 6 cut(s) 110, 318, 688, 738, 820, 881
BstAPI GCANNNNNTGC 2 cut(s) 851, 860
BstBAI YACGTR 1 cut(s) 260
BstDEI CTNAG 3 cut(s) 121, 128, 345
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 382, 517
BstFNI CGCG 4 cut(s) 154, 231, 282, 906
BstHHI GCGC 3 cut(s) 156, 233, 906
BstKTI GATC 3 cut(s) 367, 390, 650
BstMAI GTCTC 3 cut(s) 134, 307, 837
BstMBI GATC 3 cut(s) 364, 387, 647
BstMCI CGRYCG 4 cut(s) 267, 359, 390, 650
BstMWI GCNNNNNNNGC 2 cut(s) 851, 860
BstNI CCWGG 2 cut(s) 506, 610
BstSCI CCNGG 4 cut(s) 66, 353, 504, 608
BstSFI CTRYAG 1 cut(s) 59
BstSNI TACGTA 1 cut(s) 260
BstUI CGCG 4 cut(s) 154, 231, 282, 906
BstV1I GCAGC 2 cut(s) 423, 605
BstV2I GAAGAC 2 cut(s) 215, 470
BstXI CCANNNNNNTGG 1 cut(s) 421
BstZ17I GTATAC 1 cut(s) 456
BstZI CGGCCG 2 cut(s) 264, 356
BsuRI GGCC 5 cut(s) 266, 285, 358, 536, 618
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 2 cut(s) 382, 517
BtsI GCAGTG 1 cut(s) 659
BtsIMutI CAGTG 1 cut(s) 659
CfoI GCGC 3 cut(s) 156, 233, 906
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 2 cut(s) 534, 616
Cfr42I CCGCGG 1 cut(s) 283
Csp6I GTAC 3 cut(s) 125, 257, 261
CviAII CATG 2 cut(s) 573, 674
CviQI GTAC 3 cut(s) 125, 257, 261
DdeI CTNAG 3 cut(s) 121, 128, 345
DpnI GATC 3 cut(s) 366, 389, 649
DpnII GATC 3 cut(s) 364, 387, 647
DraI TTTAAA 1 cut(s) 628
EaeI YGGCCR 2 cut(s) 264, 356
EagI CGGCCG 2 cut(s) 264, 356
EclXI CGGCCG 2 cut(s) 264, 356
Eco105I TACGTA 1 cut(s) 260
Eco52I CGGCCG 2 cut(s) 264, 356
Eco57I CTGAAG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 448
EcoRI GAATTC 2 cut(s) 81, 702
EcoRII CCWGG 2 cut(s) 504, 608
EcoT22I ATGCAT 1 cut(s) 856
Esp3I CGTCTC 2 cut(s) 307, 837
FaeI CATG 2 cut(s) 576, 677
FaiI YATR 8 cut(s) 26, 50, 293, 429, 456, 482, 574, 675
FaqI GGGAC 1 cut(s) 671
FatI CATG 2 cut(s) 572, 673
FauI CCCGC 1 cut(s) 348
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 1 cut(s) 455
FokI GGATG 2 cut(s) 389, 524
FspBI CTAG 1 cut(s) 597
GlaI GCGC 3 cut(s) 155, 232, 905
GsuI CTGGAG 5 cut(s) 506, 527, 705, 758, 896
HaeIII GGCC 5 cut(s) 266, 285, 358, 536, 618
HapII CCGG 5 cut(s) 67, 182, 308, 355, 568
HhaI GCGC 3 cut(s) 156, 233, 906
Hin1II CATG 2 cut(s) 576, 677
Hin6I GCGC 3 cut(s) 154, 231, 904
HinP1I GCGC 3 cut(s) 154, 231, 904
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 4 cut(s) 44, 167, 444, 761
HpaII CCGG 5 cut(s) 67, 182, 308, 355, 568
HphI GGTGA 5 cut(s) 98, 107, 375, 487, 613
Hpy166II GTNNAC 1 cut(s) 456
Hpy188I TCNGA 3 cut(s) 348, 364, 885
Hpy188III TCNNGA 7 cut(s) 78, 93, 182, 322, 448, 722, 775
Hpy8I GTNNAC 1 cut(s) 456
HpyAV CCTTC 2 cut(s) 547, 607
HpyCH4III ACNGT 6 cut(s) 110, 318, 688, 738, 820, 881
HpyCH4IV ACGT 2 cut(s) 242, 259
HpyCH4V TGCA 6 cut(s) 562, 593, 680, 697, 815, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 851, 860
HpyF3I CTNAG 3 cut(s) 121, 128, 345
HpySE526I ACGT 2 cut(s) 242, 259
Hsp92II CATG 2 cut(s) 576, 677
HspAI GCGC 3 cut(s) 154, 231, 904
Kpn2I TCCGGA 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 364
KspI CCGCGG 1 cut(s) 283
Kzo9I GATC 3 cut(s) 364, 387, 647
LmnI GCTCC 4 cut(s) 69, 184, 300, 724
Lsp1109I GCAGC 2 cut(s) 423, 605
LweI GCATC 4 cut(s) 258, 367, 667, 891
MaeI CTAG 1 cut(s) 597
MaeII ACGT 2 cut(s) 242, 259
MalI GATC 3 cut(s) 366, 389, 649
MboI GATC 3 cut(s) 364, 387, 647
MboII GAAGA 5 cut(s) 215, 475, 737, 759, 877
MluCI AATT 9 cut(s) 51, 81, 496, 563, 629, 702, 780, 809, 894
Mph1103I ATGCAT 1 cut(s) 856
MroI TCCGGA 1 cut(s) 181
MroXI GAANNNNTTC 2 cut(s) 396, 854
MseI TTAA 1 cut(s) 627
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 5 cut(s) 67, 182, 308, 355, 568
MspR9I CCNGG 4 cut(s) 68, 355, 506, 610
Mva1269I GAATGC 2 cut(s) 854, 856
MvaI CCWGG 2 cut(s) 506, 610
MvnI CGCG 4 cut(s) 154, 231, 282, 906
MwoI GCNNNNNNNGC 2 cut(s) 851, 860
NciI CCSGG 2 cut(s) 68, 355
NdeII GATC 3 cut(s) 364, 387, 647
NlaIII CATG 2 cut(s) 576, 677
NlaIV GGNNCC 1 cut(s) 720
NmeAIII GCCGAG 1 cut(s) 279
NsiI ATGCAT 1 cut(s) 856
PaeR7I CTCGAG 1 cut(s) 448
PctI GAATGC 2 cut(s) 854, 856
PdmI GAANNNNTTC 2 cut(s) 396, 854
PfeI GAWTC 4 cut(s) 44, 167, 444, 761
Pfl23II CGTACG 2 cut(s) 256, 260
PfoI TCCNGGA 1 cut(s) 608
Ple19I CGATCG 2 cut(s) 390, 650
Ppu21I YACGTR 1 cut(s) 260
Psp6I CCWGG 2 cut(s) 504, 608
PspGI CCWGG 2 cut(s) 504, 608
PspLI CGTACG 2 cut(s) 256, 260
PspN4I GGNNCC 1 cut(s) 720
PspPI GGNCC 2 cut(s) 534, 616
PsrI GAACNNNNNNTAC 2 cut(s) 860, 892
PvuI CGATCG 2 cut(s) 390, 650
RsaI GTAC 3 cut(s) 126, 258, 262
RsaNI GTAC 3 cut(s) 125, 257, 261
RseI CAYNNNNRTG 1 cut(s) 545
SacII CCGCGG 1 cut(s) 283
SaqAI TTAA 1 cut(s) 627
Sau3AI GATC 3 cut(s) 364, 387, 647
Sau96I GGNCC 2 cut(s) 534, 616
ScaI AGTACT 1 cut(s) 126
ScrFI CCNGG 4 cut(s) 68, 355, 506, 610
SfaNI GCATC 4 cut(s) 258, 367, 667, 891
SfcI CTRYAG 1 cut(s) 59
Sfr274I CTCGAG 1 cut(s) 448
Sfr303I CCGCGG 1 cut(s) 283
SgrBI CCGCGG 1 cut(s) 283
SlaI CTCGAG 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
SnaBI TACGTA 1 cut(s) 260
Sse9I AATT 9 cut(s) 51, 81, 496, 563, 629, 702, 780, 809, 894
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 4 cut(s) 66, 353, 504, 608
TaaI ACNGT 6 cut(s) 110, 318, 688, 738, 820, 881
TaiI ACGT 2 cut(s) 245, 262
TaqI TCGA 6 cut(s) 79, 94, 212, 323, 390, 449
TaqII GACCGA 2 cut(s) 209, 482
TasI AATT 9 cut(s) 51, 81, 496, 563, 629, 702, 780, 809, 894
TatI WGTACW 1 cut(s) 124
TauI GCSGC 9 cut(s) 154, 159, 162, 236, 274, 285, 373, 621, 719
TfiI GAWTC 4 cut(s) 44, 167, 444, 761
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TscAI CASTG 1 cut(s) 659
TseI GCWGC 2 cut(s) 436, 593
TspDTI ATGAA 3 cut(s) 17, 753, 863
TspGWI ACGGA 1 cut(s) 819
TspRI CASTG 1 cut(s) 659
XapI RAATTY 4 cut(s) 81, 702, 809, 894
XhoI CTCGAG 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 455
XmnI GAANNNNTTC 2 cut(s) 396, 854
XspI CTAG 1 cut(s) 597
ZrmI AGTACT 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.