Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0016879

Physical Location & Seq

Genomic Coordinates Forward (+)

19587324 .. 19590054

Gene Structure 2731 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1111100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGTTGGGTCATGTTGGAGGCCTTCTGCAGTGGATGATGGATCTAGCCGTGGTGGGGAAGCCAGCGGCCGGGTTGATGGGTTGTTATGGTACAAGGACTTTGGGCAGCATGCTTGGGGAGAATTCTCAATGGCTGCCATTCAGGCCAATTCTGTCATCGAGGATCAAAGCCAGGTCGAATCAGGTCCTCTGTGTACAACGAAAGCCGGTCCTCTGGGGACTTTTGTTGGAGTTTATGATGGACATGGAGGGCCTGAGGCTTCAAGATACGTCAATGAAAATCTCTTCTGCAATCTAAAGAGAATTGCAGCTGAGCATCGATGCATGTCAGAAAACGTGATCAAGAAGGCGTACTTGGCGACGGAAGAAAGTTTTCTCTCCGTGGTGAAGAAGCAGTGGCAAAGCAAGCCACAAATTGCATCTGCAGGAACATGTTGTTTGGTGGGGATAATATGCGATGGACTGCTTTACACAGCAAATGTTGGGGACTCTAGGGTGGTTCTAGGAAAATTAGAAAGGGCAACTAAAGAAGTCATGGCTATTCAGTTATCTGCAGAGCACAATGCAAGCGTAGAATCCGTGAGAGAGGAACTCAAGTCGTTGCATCCACACGATTCAAAGATTGTGGTTTTAAGGCACCGAGTTTGGCGCGTTAAAGGCCTCATTCAGGTTTCGAGATCAATTGGTGACGCGTACTTGAAGAATGCAGAGTTCAACAGAGAGCCTCTACCCCTAAAATATAGGTTACCTGAACCTTTTCCCAACCCAATTCTAATCCCGGAACCAGAAGTATCAGTAATTAAACTCCGACCGGAAGATCAGTTTCTCATATTCGCTTCCGATGGTCTGTGGGAGCATCTTAGCAACCAGGAGGCTGTCGACATTGTCAACGATTACCCCCGCAAGGGAATAGCAAGGAAGCTGATTGAAGCTGCACTACAAGAAGCAGCTAAGAAAAGGGAAGTGAGGTACGCAGATTTGAGGAAAATCGAGCGCGGAGTAAGGCGACATTTTCACGACGACATCACGGTGGTGGTGGTGTTTCTGAATCAAAGCCTGTTGAAAAATGGAAGCTCCCCACGCAACTCTCATTTTTCATTAAAACCCGACTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0042578 GO:0043900 GO:0043901 GO:0044237 GO:0044424 GO:0044464 GO:0048519 GO:0048583 GO:0048585 GO:0048831 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0065007 GO:0071944 GO:0080134 GO:1900055 GO:1900056 GO:1900424 GO:1900425 GO:1905622 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

372

Amino Acids

41.37

Weight (kDa)

8.09

Isoelectric Point (pI)

43.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	73 - 318	2.8e-38	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016879)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g14920
malus_domestica	MD04G1111100.v1.1 MD12G1129700.v1.1
prunus_persica	Prupe.6G245000_v2.0.a1
pyrus_communis	pycom04g10370 pycom12g12590
rosa_chinensis	RchiOBHm_Chr3g0468121
rosa_laevigata	RLG00000024447
rosa_multiflora	Rmu_sc0003659.1_g000011
rosa_roxburghii	Rroxscaffold_6G00413010
rosa_rugosa	Rorug03G0093200
rosa_samantha	Rh3CG162700 Rh3DG163300
rosa_wichuraiana	Rw3G013350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	636
AccI	GTMKAC	1 cut(s)	879
AccII	CGCG	3 cut(s)	651, 692, 996
AciI	CCGC	3 cut(s)	66, 901, 996
AclWI	GGATC	2 cut(s)	49, 171
AcoI	YGGCCR	1 cut(s)	67
AcsI	RAATTY	1 cut(s)	122
AfaI	GTAC	5 cut(s)	92, 196, 353, 695, 971
AfiI	CCNNNNNNNGG	5 cut(s)	55, 69, 667, 904, 905
AflIII	ACRYGT	2 cut(s)	431, 690
AgsI	TTSAA	6 cut(s)	264, 618, 700, 715, 929, 1063
AjnI	CCWGG	2 cut(s)	171, 867
AjuI	GAANNNNNNNTTGG	2 cut(s)	338, 370
AleI	CACNNNNGTG	1 cut(s)	1031
AluBI	AGCT	5 cut(s)	311, 922, 932, 950, 1074
AluI	AGCT	5 cut(s)	311, 922, 932, 950, 1074
Alw21I	GWGCWC	1 cut(s)	561
AlwI	GGATC	2 cut(s)	49, 171
AoxI	GGCC	5 cut(s)	20, 67, 144, 251, 658
ApeKI	GCWGC	5 cut(s)	106, 134, 308, 932, 947
ApoI	RAATTY	1 cut(s)	122
Asp700I	GAANNNNTTC	2 cut(s)	372, 756
AspLEI	GCGC	2 cut(s)	651, 996
AspS9I	GGNCC	3 cut(s)	185, 209, 251
AsuC2I	CCSGG	2 cut(s)	71, 779
AsuHPI	GGTGA	2 cut(s)	397, 698
AvaII	GGWCC	2 cut(s)	185, 209
AxyI	CCTNAGG	1 cut(s)	255
BanI	GGYRCC	1 cut(s)	636
BarI	GAAGNNNNNNTAC	2 cut(s)	921, 953
Bbv12I	GWGCWC	1 cut(s)	561
BbvI	GCAGC	5 cut(s)	118, 121, 320, 919, 959
BccI	CCATC	5 cut(s)	32, 71, 233, 452, 836
BceAI	ACGGC	1 cut(s)	33
BciT130I	CCWGG	2 cut(s)	173, 869
BclI	TGATCA	1 cut(s)	339
BcnI	CCSGG	2 cut(s)	71, 779
BfaI	CTAG	3 cut(s)	45, 492, 503
BfmI	CTRYAG	3 cut(s)	27, 423, 552
BglI	GCCNNNNNGGC	1 cut(s)	143
BisI	GCNGC	6 cut(s)	67, 107, 135, 309, 933, 948
BlpI	GCTNAGC	1 cut(s)	312
BlsI	GCNGC	6 cut(s)	68, 108, 136, 310, 934, 949
Bme1390I	CCNGG	4 cut(s)	71, 173, 779, 869
Bme18I	GGWCC	2 cut(s)	185, 209
BmgT120I	GGNCC	3 cut(s)	185, 209, 251
BmiI	GGNNCC	2 cut(s)	638, 783
BmrFI	CCNGG	4 cut(s)	71, 173, 779, 869
BmsI	GCATC	5 cut(s)	311, 325, 428, 613, 865
BplI	GAGNNNNNCTC	2 cut(s)	576, 608
Bpu1102I	GCTNAGC	1 cut(s)	312
BpuEI	CTTGAG	1 cut(s)	578
BpuMI	CCSGG	2 cut(s)	71, 779
Bsa29I	ATCGAT	1 cut(s)	319
BsaJI	CCNNGG	2 cut(s)	49, 381
BsaWI	WCCGGW	1 cut(s)	811
Bsc4I	CCNNNNNNNGG	5 cut(s)	55, 69, 667, 904, 905
Bse118I	RCCGGY	1 cut(s)	206
Bse21I	CCTNAGG	1 cut(s)	255
BseBI	CCWGG	2 cut(s)	173, 869
BseCI	ATCGAT	1 cut(s)	319
BseDI	CCNNGG	2 cut(s)	49, 381
BseGI	GGATG	2 cut(s)	40, 604
BseLI	CCNNNNNNNGG	5 cut(s)	55, 69, 667, 904, 905
BseMII	CTCAG	2 cut(s)	246, 303
BseX3I	CGGCCG	1 cut(s)	67
BseXI	GCAGC	5 cut(s)	118, 121, 320, 919, 959
BsgI	GTGCAG	1 cut(s)	918
Bsh1236I	CGCG	3 cut(s)	651, 692, 996
Bsh1285I	CGRYCG	2 cut(s)	70, 812
BshFI	GGCC	5 cut(s)	22, 69, 146, 253, 660
BshNI	GGYRCC	1 cut(s)	636
BshVI	ATCGAT	1 cut(s)	319
BsiEI	CGRYCG	2 cut(s)	70, 812
BsiHKAI	GWGCWC	1 cut(s)	561
BsiSI	CCGG	4 cut(s)	70, 207, 779, 812
BslFI	GGGAC	2 cut(s)	232, 500
BslI	CCNNNNNNNGG	5 cut(s)	55, 69, 667, 904, 905
BsmFI	GGGAC	2 cut(s)	232, 500
BsmI	GAATGC	1 cut(s)	709
BsnI	GGCC	5 cut(s)	22, 69, 146, 253, 660
Bsp1286I	GDGCHC	1 cut(s)	561
Bsp1407I	TGTACA	1 cut(s)	194
Bsp143I	GATC	5 cut(s)	41, 163, 339, 677, 817
Bsp1720I	GCTNAGC	1 cut(s)	312
BspACI	CCGC	3 cut(s)	66, 901, 996
BspANI	GGCC	5 cut(s)	22, 69, 146, 253, 660
BspCNI	CTCAG	2 cut(s)	247, 304
BspDI	ATCGAT	1 cut(s)	319
BspFNI	CGCG	3 cut(s)	651, 692, 996
BspLI	GGNNCC	2 cut(s)	638, 783
BspMAI	CTGCAG	3 cut(s)	31, 427, 556
BspPI	GGATC	2 cut(s)	49, 171
BspT107I	GGYRCC	1 cut(s)	636
BsrFI	RCCGGY	1 cut(s)	206
BsrGI	TGTACA	1 cut(s)	194
BssAI	RCCGGY	1 cut(s)	206
BssECI	CCNNGG	2 cut(s)	49, 381
BssMI	GATC	5 cut(s)	41, 163, 339, 677, 817
Bst2UI	CCWGG	2 cut(s)	173, 869
Bst4CI	ACNGT	1 cut(s)	1030
Bst6I	CTCTTC	1 cut(s)	290
BstAUI	TGTACA	1 cut(s)	194
BstC8I	GCNNGC	4 cut(s)	64, 111, 407, 568
BstDEI	CTNAG	4 cut(s)	255, 312, 860, 951
BstDSI	CCRYGG	2 cut(s)	49, 381
BstEII	GGTNACC	1 cut(s)	744
BstENI	CCTNNNNNAGG	1 cut(s)	665
BstF5I	GGATG	2 cut(s)	40, 604
BstFNI	CGCG	3 cut(s)	651, 692, 996
BstHHI	GCGC	2 cut(s)	651, 996
BstKTI	GATC	5 cut(s)	44, 166, 342, 680, 820
BstMBI	GATC	5 cut(s)	41, 163, 339, 677, 817
BstMCI	CGRYCG	2 cut(s)	70, 812
BstMWI	GCNNNNNNNGC	5 cut(s)	143, 356, 406, 657, 1080
BstNI	CCWGG	2 cut(s)	173, 869
BstNSI	RCATGY	3 cut(s)	113, 328, 435
BstPI	GGTNACC	1 cut(s)	744
BstSCI	CCNGG	4 cut(s)	69, 171, 777, 867
BstSFI	CTRYAG	3 cut(s)	27, 423, 552
BstUI	CGCG	3 cut(s)	651, 692, 996
BstV1I	GCAGC	5 cut(s)	118, 121, 320, 919, 959
BstX2I	RGATCY	1 cut(s)	41
BstYI	RGATCY	1 cut(s)	41
BstZI	CGGCCG	1 cut(s)	67
Bsu15I	ATCGAT	1 cut(s)	319
Bsu36I	CCTNAGG	1 cut(s)	255
BsuRI	GGCC	5 cut(s)	22, 69, 146, 253, 660
BsuTUI	ATCGAT	1 cut(s)	319
BtgI	CCRYGG	2 cut(s)	49, 381
BtgZI	GCGATG	1 cut(s)	471
BtsCI	GGATG	2 cut(s)	40, 604
BtsI	GCAGTG	2 cut(s)	36, 401
BtsIMutI	CAGTG	2 cut(s)	36, 401
Cac8I	GCNNGC	4 cut(s)	64, 111, 407, 568
CfoI	GCGC	2 cut(s)	651, 996
Cfr10I	RCCGGY	1 cut(s)	206
Cfr13I	GGNCC	3 cut(s)	185, 209, 251
ClaI	ATCGAT	1 cut(s)	319
CseI	GACGC	1 cut(s)	698
Csp6I	GTAC	5 cut(s)	91, 195, 352, 694, 970
CspCI	CAANNNNNGTGG	2 cut(s)	606, 641
CviAII	CATG	6 cut(s)	12, 110, 245, 325, 432, 535
CviQI	GTAC	5 cut(s)	91, 195, 352, 694, 970
DdeI	CTNAG	4 cut(s)	255, 312, 860, 951
DpnI	GATC	5 cut(s)	43, 165, 341, 679, 819
DpnII	GATC	5 cut(s)	41, 163, 339, 677, 817
EaeI	YGGCCR	1 cut(s)	67
EagI	CGGCCG	1 cut(s)	67
Eam1104I	CTCTTC	1 cut(s)	290
EarI	CTCTTC	1 cut(s)	290
EclXI	CGGCCG	1 cut(s)	67
Eco147I	AGGCCT	2 cut(s)	22, 660
Eco47I	GGWCC	2 cut(s)	185, 209
Eco52I	CGGCCG	1 cut(s)	67
Eco81I	CCTNAGG	1 cut(s)	255
Eco91I	GGTNACC	1 cut(s)	744
EcoNI	CCTNNNNNAGG	1 cut(s)	665
EcoO109I	RGGNCCY	2 cut(s)	185, 251
EcoO65I	GGTNACC	1 cut(s)	744
EcoRI	GAATTC	1 cut(s)	122
EcoRII	CCWGG	2 cut(s)	171, 867
EcoT22I	ATGCAT	1 cut(s)	326
FaeI	CATG	6 cut(s)	15, 113, 248, 328, 435, 538
FalI	AAGNNNNNCTT	2 cut(s)	338, 370
FaqI	GGGAC	2 cut(s)	232, 500
FatI	CATG	6 cut(s)	11, 109, 244, 324, 431, 534
FauI	CCCGC	1 cut(s)	908
FbaI	TGATCA	1 cut(s)	339
FblI	GTMKAC	1 cut(s)	879
Fnu4HI	GCNGC	6 cut(s)	67, 107, 135, 309, 933, 948
FokI	GGATG	2 cut(s)	47, 591
Fsp4HI	GCNGC	6 cut(s)	67, 107, 135, 309, 933, 948
FspBI	CTAG	3 cut(s)	45, 492, 503
GlaI	GCGC	2 cut(s)	650, 995
GluI	GCNGC	6 cut(s)	67, 107, 135, 309, 933, 948
HaeIII	GGCC	5 cut(s)	22, 69, 146, 253, 660
HapII	CCGG	4 cut(s)	70, 207, 779, 812
HgaI	GACGC	1 cut(s)	698
HhaI	GCGC	2 cut(s)	651, 996
Hin1II	CATG	6 cut(s)	15, 113, 248, 328, 435, 538
Hin6I	GCGC	2 cut(s)	649, 994
HinP1I	GCGC	2 cut(s)	649, 994
HincII	GTYRAC	2 cut(s)	880, 889
HindII	GTYRAC	2 cut(s)	880, 889
HinfI	GANTC	5 cut(s)	179, 488, 575, 614, 1048
HpaII	CCGG	4 cut(s)	70, 207, 779, 812
HphI	GGTGA	2 cut(s)	397, 698
Hpy166II	GTNNAC	3 cut(s)	195, 880, 889
Hpy188I	TCNGA	4 cut(s)	331, 809, 841, 1047
Hpy188III	TCNNGA	4 cut(s)	264, 343, 675, 1016
Hpy8I	GTNNAC	3 cut(s)	195, 880, 889
Hpy99I	CGWCG	2 cut(s)	364, 1022
HpyAV	CCTTC	2 cut(s)	33, 340
HpyCH4III	ACNGT	1 cut(s)	1030
HpyCH4IV	ACGT	2 cut(s)	270, 336
HpyF10VI	GCNNNNNNNGC	5 cut(s)	143, 356, 406, 657, 1080
HpyF3I	CTNAG	4 cut(s)	255, 312, 860, 951
HpySE526I	ACGT	2 cut(s)	270, 336
Hsp92II	CATG	6 cut(s)	15, 113, 248, 328, 435, 538
HspAI	GCGC	2 cut(s)	649, 994
Ksp22I	TGATCA	1 cut(s)	339
Kzo9I	GATC	5 cut(s)	41, 163, 339, 677, 817
LmnI	GCTCC	2 cut(s)	853, 1079
Lsp1109I	GCAGC	5 cut(s)	118, 121, 320, 919, 959
LweI	GCATC	5 cut(s)	311, 325, 428, 613, 865
MaeI	CTAG	3 cut(s)	45, 492, 503
MaeII	ACGT	2 cut(s)	270, 336
MaeIII	GTNAC	2 cut(s)	686, 744
MalI	GATC	5 cut(s)	43, 165, 341, 679, 819
MboI	GATC	5 cut(s)	41, 163, 339, 677, 817
MboII	GAAGA	5 cut(s)	277, 377, 400, 712, 827
MfeI	CAATTG	1 cut(s)	681
MflI	RGATCY	1 cut(s)	41
MhlI	GDGCHC	1 cut(s)	561
MluCI	AATT	8 cut(s)	122, 148, 303, 414, 509, 681, 768, 798
MluI	ACGCGT	1 cut(s)	690
MlyI	GAGTC	1 cut(s)	482
MmeI	TCCRAC	2 cut(s)	208, 832
Mph1103I	ATGCAT	1 cut(s)	326
MroXI	GAANNNNTTC	2 cut(s)	372, 756
MseI	TTAA	4 cut(s)	632, 654, 801, 1100
MslI	CAYNNNNRTG	2 cut(s)	1028, 1031
MspA1I	CMGCKG	2 cut(s)	66, 311
MspI	CCGG	4 cut(s)	70, 207, 779, 812
MspR9I	CCNGG	4 cut(s)	71, 173, 779, 869
MunI	CAATTG	1 cut(s)	681
Mva1269I	GAATGC	1 cut(s)	709
MvaI	CCWGG	2 cut(s)	173, 869
MvnI	CGCG	3 cut(s)	651, 692, 996
MwoI	GCNNNNNNNGC	5 cut(s)	143, 356, 406, 657, 1080
NciI	CCSGG	2 cut(s)	71, 779
NdeII	GATC	5 cut(s)	41, 163, 339, 677, 817
NlaIII	CATG	6 cut(s)	15, 113, 248, 328, 435, 538
NlaIV	GGNNCC	2 cut(s)	638, 783
NmuCI	GTSAC	1 cut(s)	686
NsiI	ATGCAT	1 cut(s)	326
NspI	RCATGY	3 cut(s)	113, 328, 435
OliI	CACNNNNGTG	1 cut(s)	1031
PaeI	GCATGC	1 cut(s)	113
PceI	AGGCCT	2 cut(s)	22, 660
PciI	ACATGT	1 cut(s)	431
PctI	GAATGC	1 cut(s)	709
PdmI	GAANNNNTTC	2 cut(s)	372, 756
PfeI	GAWTC	4 cut(s)	179, 575, 614, 1048
PflFI	GACNNNGTC	1 cut(s)	884
PfoI	TCCNGGA	1 cut(s)	777
PkrI	GCNGC	6 cut(s)	68, 108, 136, 310, 934, 949
PleI	GAGTC	1 cut(s)	482
PpsI	GAGTC	1 cut(s)	482
PpuMI	RGGWCCY	1 cut(s)	185
PscI	ACATGT	1 cut(s)	431
Psp5II	RGGWCCY	1 cut(s)	185
Psp6I	CCWGG	2 cut(s)	171, 867
PspEI	GGTNACC	1 cut(s)	744
PspGI	CCWGG	2 cut(s)	171, 867
PspN4I	GGNNCC	2 cut(s)	638, 783
PspPI	GGNCC	3 cut(s)	185, 209, 251
PspPPI	RGGWCCY	1 cut(s)	185
PstI	CTGCAG	3 cut(s)	31, 427, 556
PsuI	RGATCY	1 cut(s)	41
PsyI	GACNNNGTC	1 cut(s)	884
PvuII	CAGCTG	1 cut(s)	311
RsaI	GTAC	5 cut(s)	92, 196, 353, 695, 971
RsaNI	GTAC	5 cut(s)	91, 195, 352, 694, 970
RseI	CAYNNNNRTG	2 cut(s)	1028, 1031
SalI	GTCGAC	1 cut(s)	878
SaqAI	TTAA	4 cut(s)	632, 654, 801, 1100
SatI	GCNGC	6 cut(s)	67, 107, 135, 309, 933, 948
Sau3AI	GATC	5 cut(s)	41, 163, 339, 677, 817
Sau96I	GGNCC	3 cut(s)	185, 209, 251
SchI	GAGTC	1 cut(s)	482
ScrFI	CCNGG	4 cut(s)	71, 173, 779, 869
SduI	GDGCHC	1 cut(s)	561
SfaNI	GCATC	5 cut(s)	311, 325, 428, 613, 865
SfcI	CTRYAG	3 cut(s)	27, 423, 552
SinI	GGWCC	2 cut(s)	185, 209
SmiMI	CAYNNNNRTG	2 cut(s)	1028, 1031
SmlI	CTYRAG	1 cut(s)	593
SmoI	CTYRAG	1 cut(s)	593
SphI	GCATGC	1 cut(s)	113
Sse9I	AATT	8 cut(s)	122, 148, 303, 414, 509, 681, 768, 798
SseBI	AGGCCT	2 cut(s)	22, 660
SsiI	CCGC	3 cut(s)	66, 901, 996
SspMI	CTAG	3 cut(s)	45, 492, 503
StuI	AGGCCT	2 cut(s)	22, 660
StyD4I	CCNGG	4 cut(s)	69, 171, 777, 867
TaaI	ACNGT	1 cut(s)	1030
TaiI	ACGT	2 cut(s)	273, 339
TaqI	TCGA	6 cut(s)	159, 177, 319, 674, 879, 990
TasI	AATT	8 cut(s)	122, 148, 303, 414, 509, 681, 768, 798
TatI	WGTACW	1 cut(s)	194
TauI	GCSGC	1 cut(s)	69
TfiI	GAWTC	4 cut(s)	179, 575, 614, 1048
Tru1I	TTAA	4 cut(s)	632, 654, 801, 1100
Tru9I	TTAA	4 cut(s)	632, 654, 801, 1100
TscAI	CASTG	2 cut(s)	36, 401
TseFI	GTSAC	1 cut(s)	686
TseI	GCWGC	5 cut(s)	106, 134, 308, 932, 947
Tsp45I	GTSAC	1 cut(s)	686
TspDTI	ATGAA	2 cut(s)	291, 1086
TspGWI	ACGGA	3 cut(s)	370, 377, 568
TspRI	CASTG	2 cut(s)	36, 401
Tth111I	GACNNNGTC	1 cut(s)	884
VpaK11BI	GGWCC	2 cut(s)	185, 209
XagI	CCTNNNNNAGG	1 cut(s)	665
XapI	RAATTY	1 cut(s)	122
XceI	RCATGY	3 cut(s)	113, 328, 435
XmiI	GTMKAC	1 cut(s)	879
XmnI	GAANNNNTTC	2 cut(s)	372, 756
XspI	CTAG	3 cut(s)	45, 492, 503
Zsp2I	ATGCAT	1 cut(s)	326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.