Rh3DG163300

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0016879
Physical Location & Seq
Forward (+)
14140428 .. 14143160
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG163300.1

Sequence Viewer

Length: 1143 bp
ATGATACACAAAGGTTTGCTTAAAATGGTTAAACCCTGTTGGAGGCCATCTGTAGAGGAAGATGGTGCTCGACGGGGTGGTGAAGCTAGCGGACGTGTTGAAGGGTTGTTGTGGTACAAGGACCTGGGCTACCATGCTTGGGGGCAATTTTCAATGGCTGTGATCCAAGCCAATAGTACTTTGGAGGATCAAAGCCAGCTCGAATCCGGGTTTCTGAGTGCAAACAGATTCGGTCCTCAGGGGACCTTCATTGGAGTTTACGATGGACATGGAGGCCCCGAGGCTTCAAGATATGTAAACGACAATCTTTTCAGGAATCTCCAGAGAATTGCATCAGAGCATCGAAGCATATCAGAAAATGTTATTCGAAAGGCGTTTTTGGCCACGGAAGAAAACTTCCTCTCCTTTGTGAGGAAGCAATGGCTAAGTAAGCCAGAGATTGCTTCTGCAGGAACATGTTGTTTGGTGGGAGTAGTGTGTAATGGACTTCTATACACGGCAAATGTTGGGGACTCAAGGCTGGTTCTAGGGAAAGTAGAAAGGGCCACTAGGGAAGTCATAGCTATTCAGTTGTCTTCAGAGCACAATGCAAGTATGGAATCAGTAAGACACGAACTTAAGACTACTCATCCCCATGATCCACAGATTGTGGTGTTGAGACACAAAGTTTGGCGCGTCAAAGGCCTCATACAGGTTTCAAGATCCATTGGTGATGCATATCTAAAGAATGCAGAGTTCAACAGAGAACCTCTACTACCCAAGTTTAGGTTACCAGATTCTGAACCTTTCGTCAAGCCAATTCTAAGTCCAGATCCATCAATATCGGTACTCAAACTCCGACCAGAAGATCAGTTTCTCATATTTGCTACTGATGGTCTGTGGGAGCATCTTAGCAACCAGGAGGCTGTCGACATTGTCAACAATAACCCACGTAAAGGAATTGCCAGGAAGCTTATTGAAGCTGCACTACAAGCAGCAGCAAAGAAAAGGGAAGTGAGGTACTCAGACTTGAAAAGAATCGAGCGAGGAGTGAGACGACATTTTCACGACGACATCAGTGTAGTGGTAGTGTTTCTAGATCACAATCAGTTGAAAAATGGAACCTCTCGCAAATCTAATATTTCAATAACACCCAACTTCTAG

Protein Analysis

380

Amino Acids

42.79

Weight (kDa)

9.34

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 328 2.1e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016879)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g14920
malus_domestica MD04G1111100.v1.1 MD12G1129700.v1.1
prunus_persica Prupe.6G245000_v2.0.a1
pyrus_communis pycom04g10370 pycom12g12590
rosa_chinensis RchiOBHm_Chr3g0468121
rosa_laevigata RLG00000024447
rosa_multiflora Rmu_sc0003659.1_g000011
rosa_roxburghii Rroxscaffold_6G00413010
rosa_rugosa Rorug03G0093200
rosa_samantha Rh3CG162700 Rh3DG163300
rosa_wichuraiana Rw3G013350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 909
AccII CGCG 1 cut(s) 675
AciI CCGC 1 cut(s) 90
AclWI GGATC 5 cut(s) 157, 195, 632, 696, 806
AcoI YGGCCR 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 4 cut(s) 116, 178, 828, 1001
AfiI CCNNNNNNNGG 6 cut(s) 42, 139, 411, 691, 765, 935
AflII CTTAAG 1 cut(s) 617
AflIII ACRYGT 2 cut(s) 94, 455
AgsI TTSAA 9 cut(s) 101, 153, 288, 699, 739, 959, 1012, 1093, 1125
AjiI CACGTC 1 cut(s) 95
AjnI CCWGG 3 cut(s) 123, 897, 944
AluBI AGCT 5 cut(s) 86, 199, 563, 952, 962
AluI AGCT 5 cut(s) 86, 199, 563, 952, 962
Alw21I GWGCWC 2 cut(s) 70, 585
Alw26I GTCTC 2 cut(s) 652, 1027
AlwI GGATC 5 cut(s) 157, 195, 632, 696, 806
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 5 cut(s) 44, 274, 381, 543, 682
ApeKI GCWGC 3 cut(s) 962, 974, 977
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 5 cut(s) 121, 233, 243, 275, 543
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 92, 722
AsuII TTCGAA 1 cut(s) 367
AsuNHI GCTAGC 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 3 cut(s) 121, 233, 243
AxyI CCTNAGG 1 cut(s) 237
BalI TGGCCA 1 cut(s) 383
BarI GAAGNNNNNNTAC 2 cut(s) 951, 983
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 2 cut(s) 70, 585
BbvI GCAGC 3 cut(s) 949, 986, 989
BccI CCATC 5 cut(s) 55, 56, 257, 823, 866
BceAI ACGGC 1 cut(s) 513
BciT130I CCWGG 3 cut(s) 125, 899, 946
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 652, 1027
BfaI CTAG 5 cut(s) 87, 527, 549, 1076, 1141
BfmI CTRYAG 2 cut(s) 51, 447
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 3 cut(s) 963, 975, 978
BlsI GCNGC 3 cut(s) 964, 976, 979
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 4 cut(s) 125, 208, 899, 946
Bme18I GGWCC 3 cut(s) 121, 233, 243
BmeT110I CYCGRG 1 cut(s) 278
BmgBI CACGTC 1 cut(s) 95
BmgT120I GGNCC 5 cut(s) 121, 233, 243, 275, 543
BmiI GGNNCC 3 cut(s) 244, 277, 1102
BmrFI CCNGG 4 cut(s) 125, 208, 899, 946
BmsI GCATC 4 cut(s) 341, 349, 703, 895
BmtI GCTAGC 1 cut(s) 90
BpiI GAAGAC 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 499
BpuMI CCSGG 1 cut(s) 208
BsaAI YACGTR 1 cut(s) 932
BsaBI GATNNNNATC 2 cut(s) 717, 1083
BsaJI CCNNGG 3 cut(s) 124, 279, 384
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 819, 849
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 139, 411, 691, 765, 935
Bse21I CCTNAGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 425
Bse8I GATNNNNATC 2 cut(s) 717, 1083
BseBI CCWGG 3 cut(s) 125, 899, 946
BseDI CCNNGG 3 cut(s) 124, 279, 384
BseGI GGATG 1 cut(s) 628
BseJI GATNNNNATC 2 cut(s) 717, 1083
BseLI CCNNNNNNNGG 6 cut(s) 42, 139, 411, 691, 765, 935
BseMI GCAATG 1 cut(s) 425
BseMII CTCAG 3 cut(s) 206, 251, 1017
BseRI GAGGAG 1 cut(s) 1041
BseXI GCAGC 3 cut(s) 949, 986, 989
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 5 cut(s) 46, 276, 383, 545, 684
BsiHKAI GWGCWC 2 cut(s) 70, 585
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 256, 524
BslI CCNNNNNNNGG 6 cut(s) 42, 139, 411, 691, 765, 935
BsmAI GTCTC 2 cut(s) 652, 1027
BsmBI CGTCTC 1 cut(s) 1027
BsmFI GGGAC 2 cut(s) 256, 524
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 5 cut(s) 46, 276, 383, 545, 684
BsoBI CYCGRG 1 cut(s) 278
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 70, 585
Bsp143I GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
BspACI CCGC 1 cut(s) 90
BspANI GGCC 5 cut(s) 46, 276, 383, 545, 684
BspCNI CTCAG 3 cut(s) 207, 250, 1016
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 3 cut(s) 244, 277, 1102
BspMAI CTGCAG 1 cut(s) 451
BspOI GCTAGC 1 cut(s) 90
BspPI GGATC 5 cut(s) 157, 195, 632, 696, 806
BspT104I TTCGAA 1 cut(s) 367
BspTI CTTAAG 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 425
BssECI CCNNGG 3 cut(s) 124, 279, 384
BssMI GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
Bst2UI CCWGG 3 cut(s) 125, 899, 946
BstAFI CTTAAG 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 932
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 88, 197
BstDEI CTNAG 6 cut(s) 215, 237, 425, 803, 890, 1003
BstDSI CCRYGG 1 cut(s) 384
BstEII GGTNACC 1 cut(s) 768
BstENI CCTNNNNNAGG 3 cut(s) 40, 409, 689
BstF5I GGATG 1 cut(s) 628
BstFNI CGCG 1 cut(s) 675
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 7 cut(s) 165, 190, 640, 704, 814, 850, 1081
BstMAI GTCTC 2 cut(s) 652, 1027
BstMBI GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
BstMWI GCNNNNNNNGC 4 cut(s) 380, 430, 681, 971
BstNI CCWGG 3 cut(s) 125, 899, 946
BstNSI RCATGY 1 cut(s) 459
BstPI GGTNACC 1 cut(s) 768
BstSCI CCNGG 4 cut(s) 123, 206, 897, 944
BstSFI CTRYAG 2 cut(s) 51, 447
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 949, 986, 989
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 2 cut(s) 701, 811
BstYI RGATCY 2 cut(s) 701, 811
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 5 cut(s) 46, 276, 383, 545, 684
BtgI CCRYGG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 95
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 1063
Cac8I GCNNGC 2 cut(s) 88, 197
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 1 cut(s) 675
Cfr13I GGNCC 5 cut(s) 121, 233, 243, 275, 543
CseI GACGC 1 cut(s) 664
Csp6I GTAC 4 cut(s) 115, 177, 827, 1000
CviAII CATG 4 cut(s) 134, 269, 456, 635
CviQI GTAC 4 cut(s) 115, 177, 827, 1000
DdeI CTNAG 6 cut(s) 215, 237, 425, 803, 890, 1003
DpnI GATC 7 cut(s) 164, 189, 639, 703, 813, 849, 1080
DpnII GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
EaeI YGGCCR 1 cut(s) 381
Eco147I AGGCCT 1 cut(s) 684
Eco47I GGWCC 3 cut(s) 121, 233, 243
Eco57I CTGAAG 1 cut(s) 561
Eco81I CCTNAGG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 278
Eco91I GGTNACC 1 cut(s) 768
EcoNI CCTNNNNNAGG 3 cut(s) 40, 409, 689
EcoO109I RGGNCCY 3 cut(s) 121, 243, 275
EcoO65I GGTNACC 1 cut(s) 768
EcoRII CCWGG 3 cut(s) 123, 897, 944
EcoT22I ATGCAT 1 cut(s) 718
Esp3I CGTCTC 1 cut(s) 1027
FaeI CATG 4 cut(s) 137, 272, 459, 638
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 2 cut(s) 256, 524
FatI CATG 4 cut(s) 133, 268, 455, 634
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 3 cut(s) 963, 975, 978
FokI GGATG 1 cut(s) 615
Fsp4HI GCNGC 3 cut(s) 963, 975, 978
FspBI CTAG 5 cut(s) 87, 527, 549, 1076, 1141
GlaI GCGC 1 cut(s) 674
GluI GCNGC 3 cut(s) 963, 975, 978
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 5 cut(s) 46, 276, 383, 545, 684
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 664
HhaI GCGC 1 cut(s) 675
Hin1II CATG 4 cut(s) 137, 272, 459, 638
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HincII GTYRAC 2 cut(s) 910, 919
HindII GTYRAC 2 cut(s) 910, 919
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 7 cut(s) 203, 228, 316, 512, 599, 776, 1017
HpaII CCGG 1 cut(s) 207
HphI GGTGA 2 cut(s) 92, 722
Hpy166II GTNNAC 4 cut(s) 259, 298, 910, 919
Hpy188I TCNGA 7 cut(s) 216, 337, 355, 580, 781, 839, 1006
Hpy188III TCNNGA 7 cut(s) 288, 313, 322, 699, 809, 1046, 1076
Hpy8I GTNNAC 4 cut(s) 259, 298, 910, 919
Hpy99I CGWCG 2 cut(s) 75, 1052
HpyAV CCTTC 2 cut(s) 95, 256
HpyCH4IV ACGT 2 cut(s) 94, 931
HpyCH4V TGCA 7 cut(s) 221, 332, 449, 590, 716, 731, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 430, 681, 971
HpyF3I CTNAG 6 cut(s) 215, 237, 425, 803, 890, 1003
HpySE526I ACGT 2 cut(s) 94, 931
Hsp92II CATG 4 cut(s) 137, 272, 459, 638
HspAI GCGC 1 cut(s) 673
Kzo9I GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
LmnI GCTCC 1 cut(s) 883
Lsp1109I GCAGC 3 cut(s) 949, 986, 989
LweI GCATC 4 cut(s) 341, 349, 703, 895
MaeI CTAG 5 cut(s) 87, 527, 549, 1076, 1141
MaeII ACGT 2 cut(s) 94, 931
MaeIII GTNAC 1 cut(s) 768
MalI GATC 7 cut(s) 164, 189, 639, 703, 813, 849, 1080
MboI GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
MboII GAAGA 4 cut(s) 71, 401, 567, 857
MflI RGATCY 2 cut(s) 701, 811
MhlI GDGCHC 2 cut(s) 70, 585
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 4 cut(s) 146, 327, 798, 939
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 2 cut(s) 20, 862
Mox20I TGGCCA 1 cut(s) 383
Mph1103I ATGCAT 1 cut(s) 718
MscI TGGCCA 1 cut(s) 383
MseI TTAA 3 cut(s) 21, 30, 618
MslI CAYNNNNRTG 1 cut(s) 633
Msp20I TGGCCA 1 cut(s) 383
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 4 cut(s) 125, 208, 899, 946
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 3 cut(s) 125, 899, 946
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 4 cut(s) 380, 430, 681, 971
NciI CCSGG 1 cut(s) 208
NdeII GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
NheI GCTAGC 1 cut(s) 86
NlaIII CATG 4 cut(s) 137, 272, 459, 638
NlaIV GGNNCC 3 cut(s) 244, 277, 1102
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 459
NspV TTCGAA 1 cut(s) 367
PceI AGGCCT 1 cut(s) 684
PciI ACATGT 1 cut(s) 455
PctI GAATGC 1 cut(s) 733
PfeI GAWTC 6 cut(s) 203, 228, 316, 599, 776, 1017
PflFI GACNNNGTC 1 cut(s) 914
PkrI GCNGC 3 cut(s) 964, 976, 979
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Ppu21I YACGTR 1 cut(s) 932
PpuMI RGGWCCY 2 cut(s) 121, 243
PscI ACATGT 1 cut(s) 455
Psp5II RGGWCCY 2 cut(s) 121, 243
Psp6I CCWGG 3 cut(s) 123, 897, 944
PspEI GGTNACC 1 cut(s) 768
PspGI CCWGG 3 cut(s) 123, 897, 944
PspN4I GGNNCC 3 cut(s) 244, 277, 1102
PspPI GGNCC 5 cut(s) 121, 233, 243, 275, 543
PspPPI RGGWCCY 2 cut(s) 121, 243
PsrI GAACNNNNNNTAC 2 cut(s) 738, 770
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 2 cut(s) 701, 811
PsyI GACNNNGTC 1 cut(s) 914
RsaI GTAC 4 cut(s) 116, 178, 828, 1001
RsaNI GTAC 4 cut(s) 115, 177, 827, 1000
RseI CAYNNNNRTG 1 cut(s) 633
SalI GTCGAC 1 cut(s) 908
SaqAI TTAA 3 cut(s) 21, 30, 618
SatI GCNGC 3 cut(s) 963, 975, 978
Sau3AI GATC 7 cut(s) 162, 187, 637, 701, 811, 847, 1078
Sau96I GGNCC 5 cut(s) 121, 233, 243, 275, 543
ScaI AGTACT 1 cut(s) 178
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 4 cut(s) 125, 208, 899, 946
SduI GDGCHC 2 cut(s) 70, 585
SfaNI GCATC 4 cut(s) 341, 349, 703, 895
SfcI CTRYAG 2 cut(s) 51, 447
SfuI TTCGAA 1 cut(s) 367
SinI GGWCC 3 cut(s) 121, 233, 243
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 2 cut(s) 514, 617
SmoI CTYRAG 2 cut(s) 514, 617
Sse9I AATT 4 cut(s) 146, 327, 798, 939
SseBI AGGCCT 1 cut(s) 684
SsiI CCGC 1 cut(s) 90
SspI AATATT 1 cut(s) 1120
SspMI CTAG 5 cut(s) 87, 527, 549, 1076, 1141
StuI AGGCCT 1 cut(s) 684
StyD4I CCNGG 4 cut(s) 123, 206, 897, 944
TaiI ACGT 2 cut(s) 97, 934
TaqI TCGA 6 cut(s) 70, 201, 343, 367, 909, 1020
TaqII GACCGA 1 cut(s) 221
TasI AATT 4 cut(s) 146, 327, 798, 939
TatI WGTACW 1 cut(s) 176
TfiI GAWTC 6 cut(s) 203, 228, 316, 599, 776, 1017
Tru1I TTAA 3 cut(s) 21, 30, 618
Tru9I TTAA 3 cut(s) 21, 30, 618
TscAI CASTG 1 cut(s) 1063
TseI GCWGC 3 cut(s) 962, 974, 977
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 1063
Tth111I GACNNNGTC 1 cut(s) 914
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 3 cut(s) 121, 233, 243
XagI CCTNNNNNAGG 3 cut(s) 40, 409, 689
XbaI TCTAGA 1 cut(s) 1075
XceI RCATGY 1 cut(s) 459
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 909
XspI CTAG 5 cut(s) 87, 527, 549, 1076, 1141
ZrmI AGTACT 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.