Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008420

Physical Location & Seq

Genomic Coordinates Forward (+)

20210779 .. 20217045

Gene Structure 6267 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1116800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2763 bp

ATGAATGTGGTTGGCAAAGTTGGGAGCCTAATTTCCCAAGGTGTTTACTCTGTTGCCACCCCTTTTCATCCGTTTGGCGGGGCTGTTGATGTCATTGTGGTTCAGCAGCAGGATGGGACGTTTAGGAGCACGCCTTGGTATGTCCGGTTTGGTAAATTTCAGGGTGTTCTCAAAGGTGCGGAGAAGATGGTCCACATCAATGTAAATGGCACAGATGCCAATTTCCATATGTATCTTGATAACTCGGGTGAGGCTTATTTTATAAAGGAGGTTGAATCGGGTAAAGTAAGTGAGATAAATGGGGTTGACAAGGATTCTGAAAATTCAGAAGCTACGCAAGGGGATAATACTTTCGATGGTGATGGCCAAAGTCATGACATTCGTAGACTTGAGCACAGTGTCTCTGATTCGGTGGTGGTTGAAATGAGAGAGGAACACAATTCATTTGGCGCAAAACGGATGGAGAGGGCAGAATCTGATGTTGATCGGAGGTTCTATGAATTTCAAGATGAGCAATCTTCTCTAGAGGGCTCTGTTGAGTTATCGGGGTATGGGTCTAGTCGATACGATAATCTAGACAGTGAGCGTATTGTGGAATCACAGAATTTAAACTCGGAAGTTATATTGGTGAGTGTAGATGGTCATGTATTGACAGCTCCCATCTCAGCATCAGAACAGACCGCAGAGAATGTGGAATTAGACACACCTCGATTCAATCTGGGTCCAGGTGAAGAGACGGACTTCTGCGAAGGCACCGAGGAGTTTAGTTCAGGTGAAACTGCTTGGGCTGCTGATTATATCAGTAAGCTGAATGCATCTACAGCTAGTGTTGCTTCTGGGAATGTCAGCGGCGTCCAGAAGGATGATAATGTTATTGGACATCAGTTGGAAGTTGATGAAAGAGAAGGGGAGTGCGTATGTCAAGCTCAAGAAACACAAAAGACTTCAACACAGGAAAGAGATCTTCAGATGCATAGCAATTCAGAAGACACATCTGCTAACAAGGAAGATGCTTTCAAGTGCTGTTTGGAGCTAGAAGAAAAGGCCAAACATGAAGTGAAAGATGTTGAAAATATGGGGTCCTCATTGGAGGTTCAAAGTTCACCAGAAACATCTCATGAGACTCTTCTGGTGGTTGATAGAACTGAAGATGCTACTGTTGACTTGAGAAATAATGATGAGATATCTGCATCTCCTGGTTTTGTTTCACCCGACAACAATATGCATCCACATTTGGAAGTTGGAAATGAATCAATTGAGAAGATTGTGTGTTCTGCAGAACAAATGAGTATTGACAGCATTTCTGTACATTCTGTCAGTAATGATCCAGACTGGAAGGATGGACAGTGTGTTACATCAGCAGTGGTTGATGGGACAGAAATTAATCAACAAAGACCTGAAAGTGTAGATGAATGCAGTAAAAGTGAGCTAATAGAACCCCCAACAGAATCTTCAAGCGAAGAAACACAAAATGAGCTAATAGAACCCCCACCAGAATCGTCAAGTGAAGAAATACAAGTCCATTCAAGCATAAGGTTTGAGATTTCTCTCTGTGGGAATGGACTTCGTCCGGGTATGGGGGTGAAAGCTGCTGCTGAAACCTTTGCTGCACATCGTATATCTGCAGAGGATTTTATAACTTCTGCAACATCAATTTTGAAAAATGAAAATCTGATTGTCAGATATAGGGAAAGATACTTTTCGTGGGAAAAAGCAGCTCCTGCTGTTCTTGGAATGGCTGCTTTTAATCTAGATTTATCTGTTGAGCCAGAGGAGGCAATTCCTGTCGAACAAGATGGCTCAGAAAAACCAAGGAATGAGGATCCTGGAACGCCCTCGACTCCTTCTGGACGCAGATGGAGGCTCTGGCCCATTCCATTTAGGAGGGTCAAAACACTTGAGCACACCGACAGTAATTCATCAAATGAGGATGAATTTGTTGATTCTGAATCTGGTCTGCAGAACTCACAAGTTGAAACGGTTCCTGAGTCCCCTCAGAAGCAATTTGTAAGGACAAATGTTCCCACCACCGAGCAGATAGCATCTTTAAATCTCAAAGAAGGTCAAAATATGATAACTTTCAGTTTCTCTACAAGGGTTTGGGGGACACAACAGGTTGATGCACATATATACTTGTGGAAATGGAATGCCAGAATTGTAATTTCTGATGTTGACGGAACTATTACCAAGTCGGATGTGTTAGGTCAGTTCATGCCATTAGTTGGAAAGGATTGGACGCATACTGGTGTGTCTAGGCTTTTCTCTGCGATTAAGGAAAATGGATATCAGCTACTGTTTTTGAGTGCACGTGCAATCGTACAGGCATATCTTACGAGAAGCTTTTTACTCAACCTAAAACAGGATGGAAAAGCTTTACCCAATGGACCTGTTGTTATCTCACCTGATGGATTATTTCCTTCATTGTTTCGTGAAGTTGTAAGAAGAGCACCACATGAATTCAAAATTGCCTGTTTAGAGGACATTAAAAAACTCTTCCCCGCCGATTACAATCCATTCTATGCAGGATTTGGAAATAGAGACACGGATGAGCTCAGCTACAGGAAAATTGGGATTCCAAAGGGCAAAATATTCATAATCAATCCCAAGGGTGAGGTGGCCATCAGCCATCATCCCGTTGACGTGAAGACGTACACATCTTTGCACACTCTTGTCAACGACATGTTTCCTCCAACTTCACTGGTTGAGCAGGAAGACTTCAACTCGTGGAACTACTGGAAAGTGCCATTGCCGGAAATTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000322 GO:0000325 GO:0000326 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0006810 GO:0006886 GO:0006950 GO:0007154 GO:0008104 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009247 GO:0009267 GO:0009605 GO:0009705 GO:0009987 GO:0009991 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016036 GO:0019374 GO:0019375 GO:0019637 GO:0031090 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032586 GO:0033036 GO:0033554 GO:0034613 GO:0042594 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046467 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0070727 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0090407 GO:0098588 GO:0098791 GO:0098805 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

LNS2 Lipin_N Lipin_mid

Protein Analysis

921

Amino Acids

102.15

Weight (kDa)

4.63

Isoelectric Point (pI)

44.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipin_N	PF04571	1 - 94	6.5e-31	lipin, N-terminal conserved region
Lipin_mid	PF16876	513 - 605	2.2e-17	Lipin/Ned1/Smp2 multi-domain protein middle domain
LNS2	PF08235	673 - 896	1.2e-96	LNS2 (Lipin/Ned1/Smp2)
LNS2_PITM1-3	PF24694	720 - 866	1.9e-06	PITM 1-3, LNS2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09560 AT3G09560 AT3G09560 AT3G09560 AT5G42870
fragaria_vesca	FvH4_6g14720
malus_domestica	MD04G1116800.v1.1 MD12G1132500.v1.1
prunus_persica	Prupe.6G246600_v2.0.a1 Prupe.6G246600_v2.0.a1
pyrus_communis	pycom04g10670 pycom12g12830
rosa_chinensis	RchiOBHm_Chr3g0467941
rosa_laevigata	RLG00000024465
rosa_multiflora	Rmu_sc0001793.1_g000056 Rmu_sc0006017.1_g000005
rosa_roxburghii	Rroxscaffold_6G00413270
rosa_rugosa	Rorug03G0090600
rosa_samantha	Rh3AG139100 Rh3BG160700 Rh3CG160600 Rh3DG161200
rosa_wichuraiana	Rw3G013170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	263, 1637
AccB1I	GGYRCC	1 cut(s)	752
AccB7I	CCANNNNNTGG	1 cut(s)	2222
AccI	GTMKAC	1 cut(s)	385
AciI	CCGC	5 cut(s)	78, 179, 681, 849, 2499
AclWI	GGATC	3 cut(s)	1319, 1817, 1830
AcoI	YGGCCR	2 cut(s)	364, 2616
AcsI	RAATTY	6 cut(s)	155, 322, 500, 604, 1934, 2456
AcuI	CTGAAG	2 cut(s)	950, 1167
AcvI	CACGTG	1 cut(s)	2309
AcyI	GRCGYC	1 cut(s)	852
AfaI	GTAC	3 cut(s)	1308, 2319, 2651
AfiI	CCNNNNNNNGG	4 cut(s)	77, 1577, 2222, 2359
AflIII	ACRYGT	1 cut(s)	2679
AjiI	CACGTC	1 cut(s)	2641
AjnI	CCWGG	3 cut(s)	724, 1195, 1825
AjuI	GAANNNNNNNTTGG	2 cut(s)	1435, 1467
Alw21I	GWGCWC	6 cut(s)	131, 396, 1905, 2308, 2449, 2553
Alw26I	GTCTC	4 cut(s)	406, 728, 1115, 2532
Alw44I	GTGCAC	1 cut(s)	2304
AlwI	GGATC	3 cut(s)	1319, 1817, 1830
AlwNI	CAGNNNCTG	3 cut(s)	476, 1721, 2293
Ama87I	CYCGRG	1 cut(s)	244
AoxI	GGCC	4 cut(s)	364, 1044, 1868, 2616
ApaLI	GTGCAC	1 cut(s)	2304
ApeKI	GCWGC	7 cut(s)	106, 788, 1589, 1592, 1607, 1715, 1739
ApoI	RAATTY	6 cut(s)	155, 322, 500, 604, 1934, 2456
AseI	ATTAAT	1 cut(s)	1383
Asp700I	GAANNNNTTC	1 cut(s)	1980
AspLEI	GCGC	1 cut(s)	452
AspS9I	GGNCC	5 cut(s)	190, 722, 1080, 1869, 2384
AsuC2I	CCSGG	1 cut(s)	1572
AvaI	CYCGRG	1 cut(s)	244
AvaII	GGWCC	4 cut(s)	190, 722, 1080, 2384
BaeGI	GKGCMC	1 cut(s)	2308
BalI	TGGCCA	2 cut(s)	366, 2618
BamHI	GGATCC	1 cut(s)	1822
BanI	GGYRCC	1 cut(s)	752
BanII	GRGCYC	2 cut(s)	533, 2553
BauI	CACGAG	1 cut(s)	2722
BbrPI	CACGTG	1 cut(s)	2309
BbsI	GAAGAC	3 cut(s)	993, 2651, 2718
Bbv12I	GWGCWC	6 cut(s)	131, 396, 1905, 2308, 2449, 2553
BbvI	GCAGC	7 cut(s)	118, 775, 1576, 1579, 1594, 1726, 1727
BciT130I	CCWGG	3 cut(s)	726, 1197, 1827
BcnI	CCSGG	1 cut(s)	1572
BcoDI	GTCTC	4 cut(s)	406, 728, 1115, 2532
BfaI	CTAG	7 cut(s)	524, 558, 575, 825, 1034, 1751, 2253
BfmI	CTRYAG	5 cut(s)	819, 1275, 1623, 1958, 2557
BglII	AGATCT	1 cut(s)	961
BisI	GCNGC	8 cut(s)	107, 789, 850, 1590, 1593, 1608, 1716, 1740
BlpI	GCTNAGC	1 cut(s)	2552
BlsI	GCNGC	8 cut(s)	108, 790, 851, 1591, 1594, 1609, 1717, 1741
Bme1390I	CCNGG	4 cut(s)	726, 1197, 1572, 1827
Bme18I	GGWCC	4 cut(s)	190, 722, 1080, 2384
BmeT110I	CYCGRG	1 cut(s)	244
BmgBI	CACGTC	1 cut(s)	2641
BmgT120I	GGNCC	5 cut(s)	190, 722, 1080, 1869, 2384
BmiI	GGNNCC	6 cut(s)	26, 723, 754, 1081, 1824, 1983
BmrFI	CCNGG	4 cut(s)	726, 1197, 1572, 1827
BpiI	GAAGAC	3 cut(s)	993, 2651, 2718
Bpu1102I	GCTNAGC	1 cut(s)	2552
BpuEI	CTTGAG	4 cut(s)	410, 912, 1186, 1919
BpuMI	CCSGG	1 cut(s)	1572
BsaAI	YACGTR	1 cut(s)	2309
BsaBI	GATNNNNATC	2 cut(s)	483, 2508
BsaHI	GRCGYC	1 cut(s)	852
BsaJI	CCNNGG	5 cut(s)	37, 134, 756, 1811, 2604
BsaWI	WCCGGW	1 cut(s)	144
Bsc4I	CCNNNNNNNGG	4 cut(s)	77, 1577, 2222, 2359
Bse1I	ACTGG	4 cut(s)	1337, 2248, 2703, 2738
Bse3DI	GCAATG	1 cut(s)	2744
Bse8I	GATNNNNATC	2 cut(s)	483, 2508
BseBI	CCWGG	3 cut(s)	726, 1197, 1827
BseDI	CCNNGG	5 cut(s)	37, 134, 756, 1811, 2604
BseJI	GATNNNNATC	2 cut(s)	483, 2508
BseLI	CCNNNNNNNGG	4 cut(s)	77, 1577, 2222, 2359
BseMI	GCAATG	1 cut(s)	2744
BseMII	CTCAG	5 cut(s)	678, 1815, 1977, 2009, 2566
BseNI	ACTGG	4 cut(s)	1337, 2248, 2703, 2738
BseRI	GAGGAG	2 cut(s)	773, 1787
BseSI	GKGCMC	1 cut(s)	2308
BseXI	GCAGC	7 cut(s)	118, 775, 1576, 1579, 1594, 1726, 1727
BsgI	GTGCAG	1 cut(s)	1593
BshFI	GGCC	4 cut(s)	366, 1046, 1870, 2618
BshNI	GGYRCC	1 cut(s)	752
BsiHKAI	GWGCWC	6 cut(s)	131, 396, 1905, 2308, 2449, 2553
BsiHKCI	CYCGRG	1 cut(s)	244
BsiSI	CCGG	3 cut(s)	145, 1571, 2750
BslFI	GGGAC	4 cut(s)	130, 1387, 1975, 2119
BslI	CCNNNNNNNGG	4 cut(s)	77, 1577, 2222, 2359
BsmAI	GTCTC	4 cut(s)	406, 728, 1115, 2532
BsmBI	CGTCTC	1 cut(s)	728
BsmFI	GGGAC	4 cut(s)	130, 1387, 1975, 2119
BsmI	GAATGC	3 cut(s)	817, 1418, 2152
BsnI	GGCC	4 cut(s)	366, 1046, 1870, 2618
BsoBI	CYCGRG	1 cut(s)	244
Bsp1286I	GDGCHC	7 cut(s)	131, 396, 533, 1905, 2308, 2449, 2553
Bsp1407I	TGTACA	1 cut(s)	1306
Bsp143I	GATC	4 cut(s)	484, 961, 1324, 1822
Bsp1720I	GCTNAGC	1 cut(s)	2552
BspACI	CCGC	5 cut(s)	78, 179, 681, 849, 2499
BspANI	GGCC	4 cut(s)	366, 1046, 1870, 2618
BspCNI	CTCAG	5 cut(s)	677, 1814, 1978, 2008, 2565
BspHI	TCATGA	2 cut(s)	373, 1117
BspLI	GGNNCC	6 cut(s)	26, 723, 754, 1081, 1824, 1983
BspMAI	CTGCAG	3 cut(s)	1279, 1627, 1962
BspPI	GGATC	3 cut(s)	1319, 1817, 1830
BspQI	GCTCTTC	1 cut(s)	2437
BspT107I	GGYRCC	1 cut(s)	752
BsrDI	GCAATG	1 cut(s)	2744
BsrGI	TGTACA	1 cut(s)	1306
BsrI	ACTGG	4 cut(s)	1337, 2248, 2703, 2738
BssECI	CCNNGG	5 cut(s)	37, 134, 756, 1811, 2604
BssMI	GATC	4 cut(s)	484, 961, 1324, 1822
BssNI	GRCGYC	1 cut(s)	852
BssSI	CACGAG	1 cut(s)	2722
BssT1I	CCWWGG	4 cut(s)	37, 134, 1811, 2604
Bst2BI	CACGAG	1 cut(s)	2722
Bst2UI	CCWGG	3 cut(s)	726, 1197, 1827
Bst4CI	ACNGT	7 cut(s)	398, 581, 1159, 1347, 1913, 1981, 2295
Bst6I	CTCTTC	4 cut(s)	726, 1131, 2437, 2498
BstACI	GRCGYC	1 cut(s)	852
BstAPI	GCANNNNNTGC	1 cut(s)	1721
BstAUI	TGTACA	1 cut(s)	1306
BstBAI	YACGTR	1 cut(s)	2309
BstC8I	GCNNGC	1 cut(s)	131
BstDEI	CTNAG	5 cut(s)	664, 1801, 1986, 1995, 2552
BstENI	CCTNNNNNAGG	1 cut(s)	2357
BstHHI	GCGC	1 cut(s)	452
BstKTI	GATC	4 cut(s)	487, 964, 1327, 1825
BstMAI	GTCTC	4 cut(s)	406, 728, 1115, 2532
BstMBI	GATC	4 cut(s)	484, 961, 1324, 1822
BstMWI	GCNNNNNNNGC	4 cut(s)	788, 821, 830, 1721
BstNI	CCWGG	3 cut(s)	726, 1197, 1827
BstNSI	RCATGY	1 cut(s)	2683
BstSCI	CCNGG	4 cut(s)	724, 1195, 1570, 1825
BstSFI	CTRYAG	5 cut(s)	819, 1275, 1623, 1958, 2557
BstSLI	GKGCMC	1 cut(s)	2308
BstV1I	GCAGC	7 cut(s)	118, 775, 1576, 1579, 1594, 1726, 1727
BstV2I	GAAGAC	3 cut(s)	993, 2651, 2718
BstX2I	RGATCY	2 cut(s)	961, 1822
BstYI	RGATCY	2 cut(s)	961, 1822
BsuRI	GGCC	4 cut(s)	366, 1046, 1870, 2618
BtrI	CACGTC	1 cut(s)	2641
BtsI	GCAGTG	1 cut(s)	1368
BtsIMutI	CAGTG	5 cut(s)	403, 586, 1352, 1368, 2696
Cac8I	GCNNGC	1 cut(s)	131
CaiI	CAGNNNCTG	3 cut(s)	476, 1721, 2293
CciI	TCATGA	2 cut(s)	373, 1117
CfoI	GCGC	1 cut(s)	452
Cfr13I	GGNCC	5 cut(s)	190, 722, 1080, 1869, 2384
CseI	GACGC	3 cut(s)	841, 1860, 2245
Csp6I	GTAC	3 cut(s)	1307, 2318, 2650
CviAII	CATG	7 cut(s)	374, 644, 1052, 1118, 2212, 2453, 2680
CviQI	GTAC	3 cut(s)	1307, 2318, 2650
DdeI	CTNAG	5 cut(s)	664, 1801, 1986, 1995, 2552
DpnI	GATC	4 cut(s)	486, 963, 1326, 1824
DpnII	GATC	4 cut(s)	484, 961, 1324, 1822
DraI	TTTAAA	2 cut(s)	609, 2049
EaeI	YGGCCR	2 cut(s)	364, 2616
Eam1104I	CTCTTC	4 cut(s)	726, 1131, 2437, 2498
EarI	CTCTTC	4 cut(s)	726, 1131, 2437, 2498
Ecl136II	GAGCTC	1 cut(s)	2551
Eco130I	CCWWGG	4 cut(s)	37, 134, 1811, 2604
Eco24I	GRGCYC	2 cut(s)	533, 2553
Eco32I	GATATC	2 cut(s)	1185, 2285
Eco47I	GGWCC	4 cut(s)	190, 722, 1080, 2384
Eco53kI	GAGCTC	1 cut(s)	2551
Eco57I	CTGAAG	2 cut(s)	950, 1167
Eco72I	CACGTG	1 cut(s)	2309
Eco88I	CYCGRG	1 cut(s)	244
EcoICRI	GAGCTC	1 cut(s)	2551
EcoNI	CCTNNNNNAGG	1 cut(s)	2357
EcoO109I	RGGNCCY	1 cut(s)	1080
EcoRI	GAATTC	1 cut(s)	2456
EcoRII	CCWGG	3 cut(s)	724, 1195, 1825
EcoRV	GATATC	2 cut(s)	1185, 2285
EcoT14I	CCWWGG	4 cut(s)	37, 134, 1811, 2604
EcoT22I	ATGCAT	3 cut(s)	817, 975, 1227
EcoT38I	GRGCYC	2 cut(s)	533, 2553
ErhI	CCWWGG	4 cut(s)	37, 134, 1811, 2604
Esp3I	CGTCTC	1 cut(s)	728
FaeI	CATG	7 cut(s)	377, 647, 1055, 1121, 2215, 2456, 2683
FaqI	GGGAC	4 cut(s)	130, 1387, 1975, 2119
FatI	CATG	7 cut(s)	373, 643, 1051, 1117, 2211, 2452, 2679
FauI	CCCGC	2 cut(s)	71, 2506
FauNDI	CATATG	1 cut(s)	228
FblI	GTMKAC	1 cut(s)	385
Fnu4HI	GCNGC	8 cut(s)	107, 789, 850, 1590, 1593, 1608, 1716, 1740
FriOI	GRGCYC	2 cut(s)	533, 2553
Fsp4HI	GCNGC	8 cut(s)	107, 789, 850, 1590, 1593, 1608, 1716, 1740
FspBI	CTAG	7 cut(s)	524, 558, 575, 825, 1034, 1751, 2253
GlaI	GCGC	1 cut(s)	451
GluI	GCNGC	8 cut(s)	107, 789, 850, 1590, 1593, 1608, 1716, 1740
HaeIII	GGCC	4 cut(s)	366, 1046, 1870, 2618
HapII	CCGG	3 cut(s)	145, 1571, 2750
HgaI	GACGC	3 cut(s)	841, 1860, 2245
HhaI	GCGC	1 cut(s)	452
Hin1I	GRCGYC	1 cut(s)	852
Hin1II	CATG	7 cut(s)	377, 647, 1055, 1121, 2215, 2456, 2683
Hin6I	GCGC	1 cut(s)	450
HinP1I	GCGC	1 cut(s)	450
HincII	GTYRAC	5 cut(s)	307, 1162, 2173, 2638, 2674
HindII	GTYRAC	5 cut(s)	307, 1162, 2173, 2638, 2674
HindIII	AAGCTT	2 cut(s)	2338, 2370
HpaII	CCGG	3 cut(s)	145, 1571, 2750
HpyAV	CCTTC	7 cut(s)	743, 853, 899, 1330, 1854, 2054, 2427
HpyCH4III	ACNGT	7 cut(s)	398, 581, 1159, 1347, 1913, 1981, 2295
HpyCH4IV	ACGT	4 cut(s)	119, 2308, 2640, 2648
HpyF10VI	GCNNNNNNNGC	4 cut(s)	788, 821, 830, 1721
HpyF3I	CTNAG	5 cut(s)	664, 1801, 1986, 1995, 2552
HpySE526I	ACGT	4 cut(s)	119, 2308, 2640, 2648
Hsp92I	GRCGYC	1 cut(s)	852
Hsp92II	CATG	7 cut(s)	377, 647, 1055, 1121, 2215, 2456, 2683
HspAI	GCGC	1 cut(s)	450
Kzo9I	GATC	4 cut(s)	484, 961, 1324, 1822
LguI	GCTCTTC	1 cut(s)	2437
LmnI	GCTCC	5 cut(s)	24, 126, 661, 1030, 1723
Lsp1109I	GCAGC	7 cut(s)	118, 775, 1576, 1579, 1594, 1726, 1727
MaeI	CTAG	7 cut(s)	524, 558, 575, 825, 1034, 1751, 2253
MaeII	ACGT	4 cut(s)	119, 2308, 2640, 2648
MaeIII	GTNAC	1 cut(s)	1351
MalI	GATC	4 cut(s)	486, 963, 1326, 1824
MboI	GATC	4 cut(s)	484, 961, 1324, 1822
MfeI	CAATTG	1 cut(s)	1254
MflI	RGATCY	2 cut(s)	961, 1822
MhlI	GDGCHC	7 cut(s)	131, 396, 533, 1905, 2308, 2449, 2553
MlsI	TGGCCA	2 cut(s)	366, 2618
MluNI	TGGCCA	2 cut(s)	366, 2618
MlyI	GAGTC	3 cut(s)	1117, 1834, 1997
MmeI	TCCRAC	5 cut(s)	867, 1222, 2172, 2203, 2714
Mox20I	TGGCCA	2 cut(s)	366, 2618
Mph1103I	ATGCAT	3 cut(s)	817, 975, 1227
MroXI	GAANNNNTTC	1 cut(s)	1980
MscI	TGGCCA	2 cut(s)	366, 2618
MseI	TTAA	6 cut(s)	608, 1383, 1746, 2048, 2271, 2484
MslI	CAYNNNNRTG	2 cut(s)	198, 2244
Msp20I	TGGCCA	2 cut(s)	366, 2618
MspA1I	CMGCKG	1 cut(s)	849
MspI	CCGG	3 cut(s)	145, 1571, 2750
MspR9I	CCNGG	4 cut(s)	726, 1197, 1572, 1827
MunI	CAATTG	1 cut(s)	1254
Mva1269I	GAATGC	3 cut(s)	817, 1418, 2152
MvaI	CCWGG	3 cut(s)	726, 1197, 1827
MwoI	GCNNNNNNNGC	4 cut(s)	788, 821, 830, 1721
NciI	CCSGG	1 cut(s)	1572
NdeI	CATATG	1 cut(s)	228
NdeII	GATC	4 cut(s)	484, 961, 1324, 1822
NlaIII	CATG	7 cut(s)	377, 647, 1055, 1121, 2215, 2456, 2683
NlaIV	GGNNCC	6 cut(s)	26, 723, 754, 1081, 1824, 1983
NsiI	ATGCAT	3 cut(s)	817, 975, 1227
NspI	RCATGY	1 cut(s)	2683
PagI	TCATGA	2 cut(s)	373, 1117
PciI	ACATGT	1 cut(s)	2679
PciSI	GCTCTTC	1 cut(s)	2437
PctI	GAATGC	3 cut(s)	817, 1418, 2152
PdmI	GAANNNNTTC	1 cut(s)	1980
PflFI	GACNNNGTC	1 cut(s)	1566
PflMI	CCANNNNNTGG	1 cut(s)	2222
PfoI	TCCNGGA	1 cut(s)	1825
PkrI	GCNGC	8 cut(s)	108, 790, 851, 1591, 1594, 1609, 1717, 1741
PleI	GAGTC	3 cut(s)	1117, 1834, 1996
PmaCI	CACGTG	1 cut(s)	2309
PmlI	CACGTG	1 cut(s)	2309
PpsI	GAGTC	3 cut(s)	1117, 1834, 1996
Ppu21I	YACGTR	1 cut(s)	2309
PpuMI	RGGWCCY	1 cut(s)	1080
PscI	ACATGT	1 cut(s)	2679
PshBI	ATTAAT	1 cut(s)	1383
PsiI	TTATAA	2 cut(s)	263, 1637
Psp124BI	GAGCTC	1 cut(s)	2553
Psp5II	RGGWCCY	1 cut(s)	1080
Psp6I	CCWGG	3 cut(s)	724, 1195, 1825
PspCI	CACGTG	1 cut(s)	2309
PspGI	CCWGG	3 cut(s)	724, 1195, 1825
PspN4I	GGNNCC	6 cut(s)	26, 723, 754, 1081, 1824, 1983
PspPI	GGNCC	5 cut(s)	190, 722, 1080, 1869, 2384
PspPPI	RGGWCCY	1 cut(s)	1080
PstI	CTGCAG	3 cut(s)	1279, 1627, 1962
PstNI	CAGNNNCTG	3 cut(s)	476, 1721, 2293
PsuI	RGATCY	2 cut(s)	961, 1822
PsyI	GACNNNGTC	1 cut(s)	1566
RsaI	GTAC	3 cut(s)	1308, 2319, 2651
RsaNI	GTAC	3 cut(s)	1307, 2318, 2650
RseI	CAYNNNNRTG	2 cut(s)	198, 2244
SacI	GAGCTC	1 cut(s)	2553
SapI	GCTCTTC	1 cut(s)	2437
SaqAI	TTAA	6 cut(s)	608, 1383, 1746, 2048, 2271, 2484
SatI	GCNGC	8 cut(s)	107, 789, 850, 1590, 1593, 1608, 1716, 1740
Sau3AI	GATC	4 cut(s)	484, 961, 1324, 1822
Sau96I	GGNCC	5 cut(s)	190, 722, 1080, 1869, 2384
SchI	GAGTC	3 cut(s)	1117, 1834, 1997
ScrFI	CCNGG	4 cut(s)	726, 1197, 1572, 1827
SduI	GDGCHC	7 cut(s)	131, 396, 533, 1905, 2308, 2449, 2553
SfcI	CTRYAG	5 cut(s)	819, 1275, 1623, 1958, 2557
SinI	GGWCC	4 cut(s)	190, 722, 1080, 2384
SmiMI	CAYNNNNRTG	2 cut(s)	198, 2244
SmlI	CTYRAG	4 cut(s)	389, 927, 1165, 1898
SmoI	CTYRAG	4 cut(s)	389, 927, 1165, 1898
SsiI	CCGC	5 cut(s)	78, 179, 681, 849, 2499
SspI	AATATT	1 cut(s)	2589
SspMI	CTAG	7 cut(s)	524, 558, 575, 825, 1034, 1751, 2253
SstI	GAGCTC	1 cut(s)	2553
StyD4I	CCNGG	4 cut(s)	724, 1195, 1570, 1825
StyI	CCWWGG	4 cut(s)	37, 134, 1811, 2604
TaaI	ACNGT	7 cut(s)	398, 581, 1159, 1347, 1913, 1981, 2295
TaiI	ACGT	4 cut(s)	122, 2311, 2643, 2651
TaqI	TCGA	5 cut(s)	354, 562, 709, 1788, 1838
TatI	WGTACW	1 cut(s)	1306
TauI	GCSGC	1 cut(s)	852
Tru1I	TTAA	6 cut(s)	608, 1383, 1746, 2048, 2271, 2484
Tru9I	TTAA	6 cut(s)	608, 1383, 1746, 2048, 2271, 2484
TscAI	CASTG	5 cut(s)	403, 586, 1352, 1368, 2703
TseI	GCWGC	7 cut(s)	106, 788, 1589, 1592, 1607, 1715, 1739
TspGWI	ACGGA	5 cut(s)	60, 472, 752, 2190, 2558
TspRI	CASTG	5 cut(s)	403, 586, 1352, 1368, 2703
Tth111I	GACNNNGTC	1 cut(s)	1566
Van91I	CCANNNNNTGG	1 cut(s)	2222
VneI	GTGCAC	1 cut(s)	2304
VpaK11BI	GGWCC	4 cut(s)	190, 722, 1080, 2384
VspI	ATTAAT	1 cut(s)	1383
XagI	CCTNNNNNAGG	1 cut(s)	2357
XapI	RAATTY	6 cut(s)	155, 322, 500, 604, 1934, 2456
XbaI	TCTAGA	3 cut(s)	523, 574, 1750
XceI	RCATGY	1 cut(s)	2683
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2611
XmiI	GTMKAC	1 cut(s)	385
XmnI	GAANNNNTTC	1 cut(s)	1980
XspI	CTAG	7 cut(s)	524, 558, 575, 825, 1034, 1751, 2253
Zsp2I	ATGCAT	3 cut(s)	817, 975, 1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.