Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008420

Physical Location & Seq

Genomic Coordinates Forward (+)

20769424 .. 20775010

Gene Structure 5587 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1132500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2757 bp

ATGAATGTGGTTGGCAAAGTTGGGAGCCTAATTTCCCAAGGTGTTTACTCAGTTGCCACCCCTTTTCATCCGTTCGGTGGGGCTGTTGATGTTATTGTGGTTCAGCAGCAGGATGGGACGTTTAGGAGCACGCCTTGGTATGTCCGGTTTGGTAAATTTCAGGGCGTTCTCAAAGGTGCGGAGAAGATGGTCCGCATAAATGTAAATGGCATTGATGCCAATTTCCACATGTATCTTGATAACTCGGGTGAGGCTTACTTTATAAAGGAGGTTGAGTCGGGTGAAGTAAGTGAGACAAATGGGGTTGTCAAGGATTCAGTTAATTTAGAAGCTACACAAGGGGATAATGCTTTGGATGGTGATGGCCAGAGTCATGACATTCGTAGACTTGAGCACAGTGTCTCTGATTCGGTGGTGGTTGAAATGAGAGAGGAACGCAATTCGTTTGGTGTAAACCGGATAGAGAGGGCAGAATCTGATGCTGATCGGAGGTACTATGAATTCCAAGATGAGCAATCTCCTCTAGAGGGTTCTGTTGAGTTATCGGAGTTTGGGTCGAGTCGATATGATAATTTAGACAGTGAGCATATTGTGGAATCACAGAATTTAAACTCGGGAGTTATATTGGTGAGTGTGGATGGTCATGTATTGAAAGCTCCCATCTCAGCATCAGAACAAACCTCAGAAAACGTGGAGTTAGACACACCTCGATTCAATCTGGGCCCAGGTGAAGAGGCGGACTTCTGTGAAGGCAATGAGGAGTTTTGTCCAGGTGAAACTGCTTGGGCTGATTATATCAGTAAGCTGAATGCATCTACAAGTAGTGTTGCTTCGGGTAGTATTAGTGGAGTTCGGAAGGATGATAGTGCTAATGGATATCAGTTAGAAGTTGATGAAAGAGAAGGGGAACATTCTTGTCAAACTCAAGAAACGCAGACTTCAACACAGGAAAGAGATCTTCAGATGCCTAGCTATTCAGAAGACACATCTACTAACAAGGCAGAGGTTTCCAAGAGCTGTTTGGAGCTAGAACAAATGGCCAAACATGAAGCAAAAGATGTTCAAAATACGGGGTCCTCATTGGAGGTTCAGAGTTCACCAGAAATATCTCATGAGACACTTCCGCTGGTTGATAGAACTGAAGATGCTACTGTTGACTTGAGAAATAATGTTGAGGCATCTTCAACTTTTGATTCTATTTCACCTGACAACAATATACCTCCACATTTAGAAGTTGGAAATGAATCAGTTGAAAAGATTGTGCCTTCTTCAGAACAAATAAGTATTGATAGCATTTCTTTACATTCTGTCAGTAATGATACGAACTGGAAGGATGAACAGTGTGGTACATCAGCAGTGGTTGATGGGATTGAGAGACCTGAAAGTGGAGATGAATGCAGTAAAAGTGAGCTAATAGAACCCCCAACAGAATCTTCAAGCAAAGAAACACAAAATGAGCTAATAAAACCCCCAACAGAATCGTCAAGTGAAGAAACACAAGTCCATTCAAGCATAAGGTTTGAGATTTCTCTCTGTGGGAATGAACTTCGTCCGGGTATGGGTGTGAAAGCTGCTGCTGAAACCTTTGCTGCACATCGTGTATCTGCAGAGAATTTTATAACTTCTGCAACATCAATTTTGAAAGATGAAAATTTGATTGTCAAATACAGGGAAAGATACTTTTCGTGGGAAAAAGCAGCTCCTGCCATTCTTGGAATGGCAGTATTTAGTCTAGATTTATCTGTTGAGCCCGAGGATGCAATTCCTGTCGAACAAGATGGCTCAGAAAAACAAAGGCACGAGGATCCTGGAACACCCTCTGGAACGCCCTCCACTCCTACTGGACGCAGATGGAGGCTCTGGCCCATTCCATTTAGGAGGGTCAAAACACTTGAGCACACCGACAGTAATTCATCAAATGAGGAGGAATTTGTTGATTCTGAATCTAGTCTGCAGAACTCACAAGTTGAAACAACTCCTGAGTTACCTCAGAAGCAATTTGTAAGGACAAATGTTCCCACCACCGAGCAGATAGCATCTTTAAATCTAAAAGAAGGTCAAAATATGATAACTTTCAGTTTCTCTACAAGGGTTTGGGGGACACAACAGGTTGATGCACATATTTACTTGTGGAAATGGAATGCCAGAATTGTAATTTCTGACGTGGACGGAACTATTACCAAGTCGGATGTGTTAGGTCAGTTCATGCCATTAGTTGGAAAGGATTGGACGCATACTGGTGTGTCTAGACTTTTCTCTGCGATTAAGGAAAATGGATATCAGCTACTGTTTTTGAGTGCACGTGCAATCGTACAGGCATATCTAACGAGAAGCTTTTTACTCAACCTAAAACAGGATGGAAAAGCTTTACCTAATGGTCCTGTCGTTATCTCACCCGATGGATTATTTCCTTCATTGATTCGTGAAGTTGTAAGAAGAGCACCACATGAATTCAAAATTGCCTGTTTAGAGGACATTAAAAAACTCTTCCCCGCCGATTACAATCCATTCTACGCAGGATTTGGAAATAGAGACACGGATGAGCTCAGCTACAGGAAAATTGGGATTCCAAAGGGCAAAATATTCATAATCAATCCCAAGGGTGAGGTGGCCATCAGCCATCATCCCGCTGATGTGAAGACGTACACATCTTTACACACTCTCGTCAACGACATGTTTCCTCCAACGTCATTGGTTGAGCAGGAAGACTTCAACTCGTGGAACTATTGGAAAGTGCCACTGCCGGAAATTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000322 GO:0000325 GO:0000326 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0006810 GO:0006886 GO:0006950 GO:0007154 GO:0008104 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009247 GO:0009267 GO:0009605 GO:0009705 GO:0009987 GO:0009991 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016036 GO:0019374 GO:0019375 GO:0019637 GO:0031090 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032586 GO:0033036 GO:0033554 GO:0034613 GO:0042594 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046467 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0070727 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0090407 GO:0098588 GO:0098791 GO:0098805 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

LNS2 Lipin_N Lipin_mid

Protein Analysis

919

Amino Acids

101.8

Weight (kDa)

4.67

Isoelectric Point (pI)

42.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipin_N	PF04571	1 - 95	1.9e-30	lipin, N-terminal conserved region
Lipin_mid	PF16876	507 - 598	1.1e-17	Lipin/Ned1/Smp2 multi-domain protein middle domain
LNS2	PF08235	671 - 894	8.5e-97	LNS2 (Lipin/Ned1/Smp2)
LNS2_PITM1-3	PF24694	718 - 864	2.8e-06	PITM 1-3, LNS2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09560 AT3G09560 AT3G09560 AT3G09560 AT5G42870
fragaria_vesca	FvH4_6g14720
malus_domestica	MD04G1116800.v1.1 MD12G1132500.v1.1
prunus_persica	Prupe.6G246600_v2.0.a1 Prupe.6G246600_v2.0.a1
pyrus_communis	pycom04g10670 pycom12g12830
rosa_chinensis	RchiOBHm_Chr3g0467941
rosa_laevigata	RLG00000024465
rosa_multiflora	Rmu_sc0001793.1_g000056 Rmu_sc0006017.1_g000005
rosa_roxburghii	Rroxscaffold_6G00413270
rosa_rugosa	Rorug03G0090600
rosa_samantha	Rh3AG139100 Rh3BG160700 Rh3CG160600 Rh3DG161200
rosa_wichuraiana	Rw3G013170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	263, 1619
AccB7I	CCANNNNNTGG	1 cut(s)	2216
AccI	GTMKAC	1 cut(s)	385
AciI	CCGC	6 cut(s)	179, 193, 737, 1124, 2493, 2628
AclWI	GGATC	2 cut(s)	1799, 1812
AcoI	YGGCCR	3 cut(s)	364, 1038, 2610
AcsI	RAATTY	7 cut(s)	155, 500, 604, 1612, 1651, 1928, 2450
AcuI	CTGAAG	3 cut(s)	944, 1161, 1254
AcvI	CACGTG	1 cut(s)	2303
AdeI	CACNNNGTG	1 cut(s)	1598
AfaI	GTAC	4 cut(s)	494, 1348, 2313, 2645
AfiI	CCNNNNNNNGG	6 cut(s)	77, 527, 1385, 1559, 2216, 2353
AflIII	ACRYGT	2 cut(s)	228, 2673
AjiI	CACGTC	1 cut(s)	2164
AjnI	CCWGG	3 cut(s)	724, 769, 1807
AjuI	GAANNNNNNNTTGG	2 cut(s)	1417, 1449
Alw21I	GWGCWC	6 cut(s)	131, 396, 1899, 2302, 2443, 2547
Alw26I	GTCTC	5 cut(s)	287, 406, 1109, 1369, 2526
Alw44I	GTGCAC	1 cut(s)	2298
AlwI	GGATC	2 cut(s)	1799, 1812
AlwNI	CAGNNNCTG	3 cut(s)	476, 1703, 2287
Ama87I	CYCGRG	3 cut(s)	244, 613, 1751
AoxI	GGCC	5 cut(s)	364, 721, 1038, 1862, 2610
ApaI	GGGCCC	1 cut(s)	725
ApaLI	GTGCAC	1 cut(s)	2298
ApeKI	GCWGC	5 cut(s)	106, 1571, 1574, 1589, 1697
ApoI	RAATTY	7 cut(s)	155, 500, 604, 1612, 1651, 1928, 2450
AspS9I	GGNCC	6 cut(s)	190, 721, 722, 1074, 1863, 2378
AsuC2I	CCSGG	1 cut(s)	1554
AvaI	CYCGRG	3 cut(s)	244, 613, 1751
AvaII	GGWCC	3 cut(s)	190, 1074, 2378
BaeGI	GKGCMC	2 cut(s)	725, 2302
BalI	TGGCCA	3 cut(s)	366, 1040, 2612
BamHI	GGATCC	1 cut(s)	1804
BanII	GRGCYC	3 cut(s)	725, 1752, 2547
BarI	GAAGNNNNNNTAC	2 cut(s)	814, 846
BauI	CACGAG	2 cut(s)	1799, 2716
BbrPI	CACGTG	1 cut(s)	2303
BbsI	GAAGAC	3 cut(s)	987, 2645, 2712
Bbv12I	GWGCWC	6 cut(s)	131, 396, 1899, 2302, 2443, 2547
BbvI	GCAGC	5 cut(s)	118, 1558, 1561, 1576, 1709
BciT130I	CCWGG	3 cut(s)	726, 771, 1809
BcnI	CCSGG	1 cut(s)	1554
BcoDI	GTCTC	5 cut(s)	287, 406, 1109, 1369, 2526
BfaI	CTAG	6 cut(s)	524, 969, 1028, 1733, 1947, 2247
BfmI	CTRYAG	3 cut(s)	1605, 1952, 2551
BglII	AGATCT	1 cut(s)	955
BisI	GCNGC	5 cut(s)	107, 1572, 1575, 1590, 1698
BlpI	GCTNAGC	1 cut(s)	2546
BlsI	GCNGC	5 cut(s)	108, 1573, 1576, 1591, 1699
Bme1390I	CCNGG	4 cut(s)	726, 771, 1554, 1809
Bme18I	GGWCC	3 cut(s)	190, 1074, 2378
BmeT110I	CYCGRG	3 cut(s)	244, 613, 1751
BmgBI	CACGTC	1 cut(s)	2164
BmgT120I	GGNCC	6 cut(s)	190, 721, 722, 1074, 1863, 2378
BmiI	GGNNCC	4 cut(s)	26, 723, 1075, 1806
BmrFI	CCNGG	4 cut(s)	726, 771, 1554, 1809
BpiI	GAAGAC	3 cut(s)	987, 2645, 2712
Bpu1102I	GCTNAGC	1 cut(s)	2546
BpuEI	CTTGAG	4 cut(s)	410, 909, 1180, 1913
BpuMI	CCSGG	1 cut(s)	1554
BsaAI	YACGTR	1 cut(s)	2303
BsaBI	GATNNNNATC	2 cut(s)	483, 2502
BsaI	GGTCTC	1 cut(s)	1369
BsaJI	CCNNGG	5 cut(s)	37, 134, 724, 1752, 2598
BsaWI	WCCGGW	2 cut(s)	144, 456
BsaXI	ACNNNNNCTCC	4 cut(s)	260, 290, 539, 569
Bsc4I	CCNNNNNNNGG	6 cut(s)	77, 527, 1385, 1559, 2216, 2353
Bse1I	ACTGG	3 cut(s)	1331, 1846, 2242
Bse3DI	GCAATG	1 cut(s)	760
Bse8I	GATNNNNATC	2 cut(s)	483, 2502
BseBI	CCWGG	3 cut(s)	726, 771, 1809
BseDI	CCNNGG	5 cut(s)	37, 134, 724, 1752, 2598
BseJI	GATNNNNATC	2 cut(s)	483, 2502
BseLI	CCNNNNNNNGG	6 cut(s)	77, 527, 1385, 1559, 2216, 2353
BseMI	GCAATG	1 cut(s)	760
BseMII	CTCAG	7 cut(s)	63, 678, 696, 1797, 1971, 2003, 2560
BseNI	ACTGG	3 cut(s)	1331, 1846, 2242
BseRI	GAGGAG	3 cut(s)	510, 773, 1937
BseSI	GKGCMC	2 cut(s)	725, 2302
BseXI	GCAGC	5 cut(s)	118, 1558, 1561, 1576, 1709
BsgI	GTGCAG	1 cut(s)	1575
BshFI	GGCC	5 cut(s)	366, 723, 1040, 1864, 2612
BsiHKAI	GWGCWC	6 cut(s)	131, 396, 1899, 2302, 2443, 2547
BsiHKCI	CYCGRG	3 cut(s)	244, 613, 1751
BsiSI	CCGG	4 cut(s)	145, 457, 1553, 2744
BslFI	GGGAC	2 cut(s)	130, 2113
BslI	CCNNNNNNNGG	6 cut(s)	77, 527, 1385, 1559, 2216, 2353
BsmAI	GTCTC	5 cut(s)	287, 406, 1109, 1369, 2526
BsmFI	GGGAC	2 cut(s)	130, 2113
BsmI	GAATGC	3 cut(s)	814, 1400, 2146
BsnI	GGCC	5 cut(s)	366, 723, 1040, 1864, 2612
Bso31I	GGTCTC	1 cut(s)	1369
BsoBI	CYCGRG	3 cut(s)	244, 613, 1751
Bsp120I	GGGCCC	1 cut(s)	721
Bsp1286I	GDGCHC	8 cut(s)	131, 396, 725, 1752, 1899, 2302, 2443, 2547
Bsp143I	GATC	3 cut(s)	484, 955, 1804
Bsp1720I	GCTNAGC	1 cut(s)	2546
BspACI	CCGC	6 cut(s)	179, 193, 737, 1124, 2493, 2628
BspANI	GGCC	5 cut(s)	366, 723, 1040, 1864, 2612
BspCNI	CTCAG	7 cut(s)	62, 677, 695, 1796, 1972, 2002, 2559
BspHI	TCATGA	2 cut(s)	373, 1111
BspLI	GGNNCC	4 cut(s)	26, 723, 1075, 1806
BspMAI	CTGCAG	2 cut(s)	1609, 1956
BspPI	GGATC	2 cut(s)	1799, 1812
BspQI	GCTCTTC	1 cut(s)	2431
BspTNI	GGTCTC	1 cut(s)	1369
BsrDI	GCAATG	1 cut(s)	760
BsrI	ACTGG	3 cut(s)	1331, 1846, 2242
BssECI	CCNNGG	5 cut(s)	37, 134, 724, 1752, 2598
BssMI	GATC	3 cut(s)	484, 955, 1804
BssSI	CACGAG	2 cut(s)	1799, 2716
BssT1I	CCWWGG	3 cut(s)	37, 134, 2598
Bst2BI	CACGAG	2 cut(s)	1799, 2716
Bst2UI	CCWGG	3 cut(s)	726, 771, 1809
Bst4CI	ACNGT	6 cut(s)	398, 581, 1153, 1341, 1907, 2289
Bst6I	CTCTTC	3 cut(s)	726, 2431, 2492
BstAPI	GCANNNNNTGC	1 cut(s)	1703
BstBAI	YACGTR	1 cut(s)	2303
BstC8I	GCNNGC	1 cut(s)	131
BstDEI	CTNAG	7 cut(s)	49, 664, 682, 1783, 1980, 1989, 2546
BstENI	CCTNNNNNAGG	1 cut(s)	2351
BstKTI	GATC	3 cut(s)	487, 958, 1807
BstMAI	GTCTC	5 cut(s)	287, 406, 1109, 1369, 2526
BstMBI	GATC	3 cut(s)	484, 955, 1804
BstMWI	GCNNNNNNNGC	1 cut(s)	1703
BstNI	CCWGG	3 cut(s)	726, 771, 1809
BstNSI	RCATGY	2 cut(s)	232, 2677
BstSCI	CCNGG	4 cut(s)	724, 769, 1552, 1807
BstSFI	CTRYAG	3 cut(s)	1605, 1952, 2551
BstSLI	GKGCMC	2 cut(s)	725, 2302
BstV1I	GCAGC	5 cut(s)	118, 1558, 1561, 1576, 1709
BstV2I	GAAGAC	3 cut(s)	987, 2645, 2712
BstX2I	RGATCY	2 cut(s)	955, 1804
BstYI	RGATCY	2 cut(s)	955, 1804
BsuRI	GGCC	5 cut(s)	366, 723, 1040, 1864, 2612
BtrI	CACGTC	1 cut(s)	2164
BtsI	GCAGTG	2 cut(s)	1362, 2738
BtsIMutI	CAGTG	5 cut(s)	403, 586, 1346, 1362, 2738
Cac8I	GCNNGC	1 cut(s)	131
CaiI	CAGNNNCTG	3 cut(s)	476, 1703, 2287
CciI	TCATGA	2 cut(s)	373, 1111
Cfr13I	GGNCC	6 cut(s)	190, 721, 722, 1074, 1863, 2378
CseI	GACGC	2 cut(s)	1854, 2239
Csp6I	GTAC	4 cut(s)	493, 1347, 2312, 2644
CviAII	CATG	8 cut(s)	229, 374, 644, 1046, 1112, 2206, 2447, 2674
CviQI	GTAC	4 cut(s)	493, 1347, 2312, 2644
DdeI	CTNAG	7 cut(s)	49, 664, 682, 1783, 1980, 1989, 2546
DpnI	GATC	3 cut(s)	486, 957, 1806
DpnII	GATC	3 cut(s)	484, 955, 1804
DraI	TTTAAA	2 cut(s)	609, 2043
DraIII	CACNNNGTG	1 cut(s)	1598
EaeI	YGGCCR	3 cut(s)	364, 1038, 2610
Eam1104I	CTCTTC	3 cut(s)	726, 2431, 2492
EarI	CTCTTC	3 cut(s)	726, 2431, 2492
EciI	GGCGGA	1 cut(s)	752
Ecl136II	GAGCTC	1 cut(s)	2545
Eco130I	CCWWGG	3 cut(s)	37, 134, 2598
Eco24I	GRGCYC	3 cut(s)	725, 1752, 2547
Eco31I	GGTCTC	1 cut(s)	1369
Eco32I	GATATC	2 cut(s)	878, 2279
Eco47I	GGWCC	3 cut(s)	190, 1074, 2378
Eco53kI	GAGCTC	1 cut(s)	2545
Eco57I	CTGAAG	3 cut(s)	944, 1161, 1254
Eco72I	CACGTG	1 cut(s)	2303
Eco88I	CYCGRG	3 cut(s)	244, 613, 1751
EcoICRI	GAGCTC	1 cut(s)	2545
EcoNI	CCTNNNNNAGG	1 cut(s)	2351
EcoO109I	RGGNCCY	1 cut(s)	1074
EcoRI	GAATTC	2 cut(s)	500, 2450
EcoRII	CCWGG	3 cut(s)	724, 769, 1807
EcoRV	GATATC	2 cut(s)	878, 2279
EcoT14I	CCWWGG	3 cut(s)	37, 134, 2598
EcoT22I	ATGCAT	1 cut(s)	814
EcoT38I	GRGCYC	3 cut(s)	725, 1752, 2547
ErhI	CCWWGG	3 cut(s)	37, 134, 2598
FaeI	CATG	8 cut(s)	232, 377, 647, 1049, 1115, 2209, 2450, 2677
FaqI	GGGAC	2 cut(s)	130, 2113
FatI	CATG	8 cut(s)	228, 373, 643, 1045, 1111, 2205, 2446, 2673
FauI	CCCGC	2 cut(s)	2500, 2635
FblI	GTMKAC	1 cut(s)	385
Fnu4HI	GCNGC	5 cut(s)	107, 1572, 1575, 1590, 1698
FriOI	GRGCYC	3 cut(s)	725, 1752, 2547
Fsp4HI	GCNGC	5 cut(s)	107, 1572, 1575, 1590, 1698
FspBI	CTAG	6 cut(s)	524, 969, 1028, 1733, 1947, 2247
GluI	GCNGC	5 cut(s)	107, 1572, 1575, 1590, 1698
HaeIII	GGCC	5 cut(s)	366, 723, 1040, 1864, 2612
HapII	CCGG	4 cut(s)	145, 457, 1553, 2744
HgaI	GACGC	2 cut(s)	1854, 2239
Hin1II	CATG	8 cut(s)	232, 377, 647, 1049, 1115, 2209, 2450, 2677
HincII	GTYRAC	2 cut(s)	1156, 2668
HindII	GTYRAC	2 cut(s)	1156, 2668
HindIII	AAGCTT	2 cut(s)	2332, 2364
HpaII	CCGG	4 cut(s)	145, 457, 1553, 2744
Hpy166II	GTNNAC	9 cut(s)	46, 386, 454, 1097, 1156, 2167, 2300, 2646, 2668
Hpy8I	GTNNAC	9 cut(s)	46, 386, 454, 1097, 1156, 2167, 2300, 2646, 2668
HpyAV	CCTTC	7 cut(s)	743, 850, 896, 1275, 1324, 2048, 2421
HpyCH4III	ACNGT	6 cut(s)	398, 581, 1153, 1341, 1907, 2289
HpyCH4IV	ACGT	6 cut(s)	119, 690, 2163, 2302, 2642, 2687
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1703
HpyF3I	CTNAG	7 cut(s)	49, 664, 682, 1783, 1980, 1989, 2546
HpySE526I	ACGT	6 cut(s)	119, 690, 2163, 2302, 2642, 2687
Hsp92II	CATG	8 cut(s)	232, 377, 647, 1049, 1115, 2209, 2450, 2677
Kzo9I	GATC	3 cut(s)	484, 955, 1804
LguI	GCTCTTC	1 cut(s)	2431
LmnI	GCTCC	5 cut(s)	24, 126, 661, 1024, 1705
Lsp1109I	GCAGC	5 cut(s)	118, 1558, 1561, 1576, 1709
MaeI	CTAG	6 cut(s)	524, 969, 1028, 1733, 1947, 2247
MaeII	ACGT	6 cut(s)	119, 690, 2163, 2302, 2642, 2687
MaeIII	GTNAC	1 cut(s)	1983
MalI	GATC	3 cut(s)	486, 957, 1806
MboI	GATC	3 cut(s)	484, 955, 1804
MflI	RGATCY	2 cut(s)	955, 1804
MhlI	GDGCHC	8 cut(s)	131, 396, 725, 1752, 1899, 2302, 2443, 2547
MlsI	TGGCCA	3 cut(s)	366, 1040, 2612
MluNI	TGGCCA	3 cut(s)	366, 1040, 2612
MlyI	GAGTC	3 cut(s)	284, 379, 568
MmeI	TCCRAC	4 cut(s)	1216, 2166, 2197, 2708
Mox20I	TGGCCA	3 cut(s)	366, 1040, 2612
Mph1103I	ATGCAT	1 cut(s)	814
MscI	TGGCCA	3 cut(s)	366, 1040, 2612
MseI	TTAA	5 cut(s)	321, 608, 2042, 2265, 2478
MslI	CAYNNNNRTG	1 cut(s)	2238
Msp20I	TGGCCA	3 cut(s)	366, 1040, 2612
MspA1I	CMGCKG	2 cut(s)	1126, 2630
MspI	CCGG	4 cut(s)	145, 457, 1553, 2744
MspR9I	CCNGG	4 cut(s)	726, 771, 1554, 1809
Mva1269I	GAATGC	3 cut(s)	814, 1400, 2146
MvaI	CCWGG	3 cut(s)	726, 771, 1809
MwoI	GCNNNNNNNGC	1 cut(s)	1703
NciI	CCSGG	1 cut(s)	1554
NdeII	GATC	3 cut(s)	484, 955, 1804
NlaIII	CATG	8 cut(s)	232, 377, 647, 1049, 1115, 2209, 2450, 2677
NlaIV	GGNNCC	4 cut(s)	26, 723, 1075, 1806
NsiI	ATGCAT	1 cut(s)	814
NspI	RCATGY	2 cut(s)	232, 2677
PagI	TCATGA	2 cut(s)	373, 1111
PciI	ACATGT	2 cut(s)	228, 2673
PciSI	GCTCTTC	1 cut(s)	2431
PctI	GAATGC	3 cut(s)	814, 1400, 2146
PflMI	CCANNNNNTGG	1 cut(s)	2216
PfoI	TCCNGGA	1 cut(s)	1807
PkrI	GCNGC	5 cut(s)	108, 1573, 1576, 1591, 1699
PleI	GAGTC	3 cut(s)	283, 378, 567
PmaCI	CACGTG	1 cut(s)	2303
PmlI	CACGTG	1 cut(s)	2303
PpsI	GAGTC	3 cut(s)	283, 378, 567
Ppu21I	YACGTR	1 cut(s)	2303
PpuMI	RGGWCCY	1 cut(s)	1074
PscI	ACATGT	2 cut(s)	228, 2673
PsiI	TTATAA	2 cut(s)	263, 1619
Psp124BI	GAGCTC	1 cut(s)	2547
Psp5II	RGGWCCY	1 cut(s)	1074
Psp6I	CCWGG	3 cut(s)	724, 769, 1807
PspCI	CACGTG	1 cut(s)	2303
PspGI	CCWGG	3 cut(s)	724, 769, 1807
PspN4I	GGNNCC	4 cut(s)	26, 723, 1075, 1806
PspOMI	GGGCCC	1 cut(s)	721
PspPI	GGNCC	6 cut(s)	190, 721, 722, 1074, 1863, 2378
PspPPI	RGGWCCY	1 cut(s)	1074
PstI	CTGCAG	2 cut(s)	1609, 1956
PstNI	CAGNNNCTG	3 cut(s)	476, 1703, 2287
PsuI	RGATCY	2 cut(s)	955, 1804
RsaI	GTAC	4 cut(s)	494, 1348, 2313, 2645
RsaNI	GTAC	4 cut(s)	493, 1347, 2312, 2644
RseI	CAYNNNNRTG	1 cut(s)	2238
SacI	GAGCTC	1 cut(s)	2547
SapI	GCTCTTC	1 cut(s)	2431
SaqAI	TTAA	5 cut(s)	321, 608, 2042, 2265, 2478
SatI	GCNGC	5 cut(s)	107, 1572, 1575, 1590, 1698
Sau3AI	GATC	3 cut(s)	484, 955, 1804
Sau96I	GGNCC	6 cut(s)	190, 721, 722, 1074, 1863, 2378
SchI	GAGTC	3 cut(s)	284, 379, 568
ScrFI	CCNGG	4 cut(s)	726, 771, 1554, 1809
SduI	GDGCHC	8 cut(s)	131, 396, 725, 1752, 1899, 2302, 2443, 2547
SfcI	CTRYAG	3 cut(s)	1605, 1952, 2551
SinI	GGWCC	3 cut(s)	190, 1074, 2378
SmiMI	CAYNNNNRTG	1 cut(s)	2238
SmlI	CTYRAG	4 cut(s)	389, 924, 1159, 1892
SmoI	CTYRAG	4 cut(s)	389, 924, 1159, 1892
SsiI	CCGC	6 cut(s)	179, 193, 737, 1124, 2493, 2628
SspI	AATATT	1 cut(s)	2583
SspMI	CTAG	6 cut(s)	524, 969, 1028, 1733, 1947, 2247
SstI	GAGCTC	1 cut(s)	2547
StyD4I	CCNGG	4 cut(s)	724, 769, 1552, 1807
StyI	CCWWGG	3 cut(s)	37, 134, 2598
TaaI	ACNGT	6 cut(s)	398, 581, 1153, 1341, 1907, 2289
TaiI	ACGT	6 cut(s)	122, 693, 2166, 2305, 2645, 2690
TaqI	TCGA	4 cut(s)	557, 562, 709, 1770
Tru1I	TTAA	5 cut(s)	321, 608, 2042, 2265, 2478
Tru9I	TTAA	5 cut(s)	321, 608, 2042, 2265, 2478
TscAI	CASTG	5 cut(s)	403, 586, 1346, 1362, 2745
TseI	GCWGC	5 cut(s)	106, 1571, 1574, 1589, 1697
TspGWI	ACGGA	3 cut(s)	60, 2184, 2552
TspRI	CASTG	5 cut(s)	403, 586, 1346, 1362, 2745
Van91I	CCANNNNNTGG	1 cut(s)	2216
VneI	GTGCAC	1 cut(s)	2298
VpaK11BI	GGWCC	3 cut(s)	190, 1074, 2378
XagI	CCTNNNNNAGG	1 cut(s)	2351
XapI	RAATTY	7 cut(s)	155, 500, 604, 1612, 1651, 1928, 2450
XbaI	TCTAGA	3 cut(s)	523, 1732, 2246
XceI	RCATGY	2 cut(s)	232, 2677
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1018, 1714, 2605
XmiI	GTMKAC	1 cut(s)	385
XspI	CTAG	6 cut(s)	524, 969, 1028, 1733, 1947, 2247
Zsp2I	ATGCAT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.