MD04G1117500.v1.1

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008293
Physical Location & Seq
Reverse (-)
20264923 .. 20269063
4141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1117500.v1.1.491

Sequence Viewer

Length: 1851 bp
ATGAGAATCCGTTTGGAACCGGGGCATGATTCAGGTGAAGGGGACCTGGAGAAGGGGCTGGCGCAGCCCTCGACCCCAGGAGCTCTGTCGGAGCCGCCGTCGTCGCCGCACCCGTCGAGGCCGGCCCTGGTGTTCTCCAATTCCAGCAAGGCCCTACTGGTTTCAAATTCCGGCAAGGCATTGCTGGTTTCAAATTCCGGCAAGGCATTGCTGGTTTCAAATTCCGGCAAGTCTCTGGTCGTGTCGAATTCCGGGAAGCCCCTTGATCAGTTGGGGAGGAAGAAGTATGTGAAGCAGGTGACCGGGCGCAACAATGACACGGAGCTTCACCTGGCTGCGCGCCGCGGGGATTTGGCAGCGCTGCAGCAGATTCTAGCGGAGATTGACGCTCAGATGGTGGGATCCGAGTTTGATGAAGAGGTGGCGGAGATTAGGTCTGCCATTGTGAATGAGGTGAATGAGTTGGGAGAGACGGCGCTTTTCATAGCGGCGGAGCAGGGGCATCTTGCTATGGTGGAGGAGCTTTTGCCTTACATGACGGAGGAGGGCATTTTGTTGAAGAATCAAATTGGGTTTGATGCATTGCATATTGCTGCAAATCAAGGGCACCAAGCCATTGTCCAGGTGCTCTTAGACCATGACCCGGGGCTTAGCAAAACAGTTGGGCAGGCAAATGCGACCCCTCTTGTGTCTGCAGCTACAAGAGGGCATACAGCAGTAGTTAAAGAATTACTATCCAAAGATTCAAGTTTGCTGGAATTAGCGAAATCCAATGGGAAGAATGCATTGCATCTTGCTGCACGGCAGGGGCATGTAGAAATTGTGAAAACTTTGCTTGGAAGGGATCCACAACTAGCAAGAAGAACTGATAAGAAAGGACAAACTGCTTTGCATATGGCAGTAAAAGGTGTTAGCAGTGAAGTTGTGAGGTTGATTGTCGATGCGGATCCAGCTATTGTCATGCTTCCAGACAAGTTCGGCAATTCAGCATTGCATGTGGCCACCAGGAAAAAGCGAGTAGATATAGTCAATGTGCTATTGCTCGTTCATGACACAAATGTGAATGTTCTAACAAGAGACCAGAAAACACCCCTTGATATAGCCGAAGGACTTCCATACTCTGAAGAAATCACAACAATTAAGGAAACCTTGACTCAATATGGTGCTGTCCGAGCAAATGAACTTAACCAGCCACGGGATGAACTTAGAAACACCGTGACAGAGATTAAAAAAAATGTCCATAATCAGCTTGAACAAGCCCGCAAAACCAACCAGAGTGTAACTGGAATTGCCACAGAGCTCCGGAAGCTACAGAGGGAAGGAATCAATAATGCCACTAACTCAGTCACAGTGGTTGCTTCACTCTTTGCAACAGTAGCTTTTGCAGCTCTATTTACACTTCCTGGTGGTGATCTTGACTCCGGGGCAGCTGTTATGTCAATCCAAGTGTCATTTAAAATTTTCATCCTTTTCAATGCTGTTTCACTGTTCGCATCATTGGCTGTTGTTTTGGTACAAATCACCCTCGTCAGGGGGGAGACAAAGGCGGAGAGGAGGGTTGTGGAAGTGATCAATAAGTTGATGTGGCTTGCCTCTGTCTGCACCACAGTAGCATTTATTTCTTCATCATATATAGTGGTTGGGCGGCATAATGTGTGGGCTGCAATCCTTGTCACAGTTATAGGGGGACTTATAATGGGGGGAGTTATTGGTACCATGACATATTATGTGGTGAAGACCAAAAGGATTCGTAGAGTGAGGAAGAGGGAGAAGATGTCGAGGATTGGAACCAACCATTCCGTGCATCACTCAGAGTCCGATTCAGAAGTGAACCGTGTTTATGCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

617

Amino Acids

66.57

Weight (kDa)

9.16

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 109 - 214 1.6e-13 Ankyrin repeats (3 copies)
Ank_2 PF12796 195 - 282 5.6e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 230 - 279 2.4e-07 Ankyrin repeats (many copies)
Ank PF00023 258 - 283 7.3e-06 Ankyrin repeat
Ank_4 PF13637 263 - 310 2.4e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 289 - 355 1.2e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 313 - 368 1.6e-06 Ankyrin repeats (many copies)
PGG PF13962 440 - 546 1.3e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1694
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
Acc65I GGTACC 1 cut(s) 1712
AccB1I GGYRCC 2 cut(s) 606, 1712
AccII CGCG 2 cut(s) 340, 345
AccIII TCCGGA 1 cut(s) 1302
AclWI GGATC 6 cut(s) 396, 409, 839, 852, 941, 954
AcoI YGGCCR 1 cut(s) 999
AcsI RAATTY 5 cut(s) 166, 193, 220, 247, 1458
AcuI CTGAAG 1 cut(s) 1143
AfaI GTAC 2 cut(s) 1515, 1714
AfeI AGCGCT 1 cut(s) 360
AfiI CCNNNNNNNGG 5 cut(s) 52, 643, 1195, 1530, 1531
AgsI TTSAA 7 cut(s) 165, 192, 219, 559, 747, 1253, 1474
AjnI CCWGG 7 cut(s) 45, 76, 126, 330, 621, 1004, 1402
AleI CACNNNNGTG 1 cut(s) 1058
Alw21I GWGCWC 3 cut(s) 85, 630, 1302
Alw26I GTCTC 4 cut(s) 237, 464, 1071, 1532
AlwI GGATC 6 cut(s) 396, 409, 839, 852, 941, 954
Ama87I CYCGRG 1 cut(s) 643
Aor13HI TCCGGA 1 cut(s) 1302
Aor51HI AGCGCT 1 cut(s) 360
AoxI GGCC 4 cut(s) 119, 123, 150, 999
ApoI RAATTY 5 cut(s) 166, 193, 220, 247, 1458
Asp700I GAANNNNTTC 1 cut(s) 1110
Asp718I GGTACC 1 cut(s) 1712
AspLEI GCGC 6 cut(s) 64, 309, 340, 342, 361, 478
AspS9I GGNCC 3 cut(s) 43, 124, 151
AsuC2I CCSGG 6 cut(s) 21, 253, 304, 644, 645, 1423
AsuHPI GGTGA 7 cut(s) 47, 310, 320, 466, 1421, 1513, 1744
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 609
BalI TGGCCA 1 cut(s) 1001
BamHI GGATCC 3 cut(s) 401, 844, 946
BanI GGYRCC 2 cut(s) 606, 1712
BanII GRGCYC 2 cut(s) 85, 1302
BbsI GAAGAC 1 cut(s) 1742
Bbv12I GWGCWC 3 cut(s) 85, 630, 1302
BccI CCATC 1 cut(s) 388
BceAI ACGGC 3 cut(s) 82, 489, 818
BciT130I CCWGG 7 cut(s) 47, 78, 128, 332, 623, 1006, 1404
BclI TGATCA 2 cut(s) 265, 1569
BcnI CCSGG 6 cut(s) 21, 253, 304, 644, 645, 1423
BcoDI GTCTC 4 cut(s) 237, 464, 1071, 1532
BfaI CTAG 2 cut(s) 374, 854
BfmI CTRYAG 3 cut(s) 362, 693, 1310
BfoI RGCGCY 2 cut(s) 362, 479
BfuAI ACCTGC 1 cut(s) 286
BlpI GCTNAGC 1 cut(s) 650
Bme18I GGWCC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 3 cut(s) 43, 124, 151
BmiI GGNNCC 9 cut(s) 18, 44, 93, 403, 608, 846, 948, 1714, 1789
BmsI GCATC 6 cut(s) 511, 568, 799, 931, 1502, 1813
BpiI GAAGAC 1 cut(s) 1742
BpmI CTGGAG 1 cut(s) 68
Bpu1102I GCTNAGC 1 cut(s) 650
BpuMI CCSGG 6 cut(s) 21, 253, 304, 644, 645, 1423
BsaBI GATNNNNATC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 1071
BsaJI CCNNGG 8 cut(s) 20, 76, 126, 343, 643, 644, 1193, 1422
BsaWI WCCGGW 1 cut(s) 1302
BsaXI ACNNNNNCTCC 6 cut(s) 83, 113, 419, 449, 1760, 1790
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 643, 1195, 1530, 1531
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 2 cut(s) 162, 1288
Bse3DI GCAATG 5 cut(s) 179, 206, 581, 785, 989
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 1302
BseBI CCWGG 7 cut(s) 47, 78, 128, 332, 623, 1006, 1404
BseDI CCNNGG 8 cut(s) 20, 76, 126, 343, 643, 644, 1193, 1422
BseGI GGATG 2 cut(s) 1204, 1464
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 5 cut(s) 52, 643, 1195, 1530, 1531
BseMI GCAATG 5 cut(s) 179, 206, 581, 785, 989
BseMII CTCAG 3 cut(s) 404, 1356, 1824
BseNI ACTGG 2 cut(s) 162, 1288
BsePI GCGCGC 1 cut(s) 338
BseRI GAGGAG 3 cut(s) 533, 557, 1567
BseSI GKGCMC 1 cut(s) 609
BsgI GTGCAG 2 cut(s) 783, 1585
Bsh1236I CGCG 2 cut(s) 340, 345
BshFI GGCC 4 cut(s) 121, 125, 152, 1001
BshNI GGYRCC 2 cut(s) 606, 1712
BsiHKAI GWGCWC 3 cut(s) 85, 630, 1302
BsiHKCI CYCGRG 1 cut(s) 643
BslFI GGGAC 2 cut(s) 56, 1701
BslI CCNNNNNNNGG 5 cut(s) 52, 643, 1195, 1530, 1531
BsmAI GTCTC 4 cut(s) 237, 464, 1071, 1532
BsmBI CGTCTC 1 cut(s) 464
BsmFI GGGAC 2 cut(s) 56, 1701
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 4 cut(s) 121, 125, 152, 1001
Bso31I GGTCTC 1 cut(s) 1071
BsoBI CYCGRG 1 cut(s) 643
Bsp1286I GDGCHC 4 cut(s) 85, 609, 630, 1302
Bsp13I TCCGGA 1 cut(s) 1302
Bsp143I GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
Bsp1720I GCTNAGC 1 cut(s) 650
BspANI GGCC 4 cut(s) 121, 125, 152, 1001
BspCNI CTCAG 3 cut(s) 403, 1355, 1823
BspEI TCCGGA 1 cut(s) 1302
BspFNI CGCG 2 cut(s) 340, 345
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 9 cut(s) 18, 44, 93, 403, 608, 846, 948, 1714, 1789
BspMAI CTGCAG 2 cut(s) 366, 697
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 6 cut(s) 396, 409, 839, 852, 941, 954
BspT107I GGYRCC 2 cut(s) 606, 1712
BspTNI GGTCTC 1 cut(s) 1071
BsrDI GCAATG 5 cut(s) 179, 206, 581, 785, 989
BsrFI RCCGGY 1 cut(s) 121
BsrI ACTGG 2 cut(s) 162, 1288
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 8 cut(s) 20, 76, 126, 343, 643, 644, 1193, 1422
BssHII GCGCGC 1 cut(s) 338
BssMI GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
Bst2UI CCWGG 7 cut(s) 47, 78, 128, 332, 623, 1006, 1404
Bst4CI ACNGT 8 cut(s) 661, 1216, 1351, 1375, 1488, 1609, 1678, 1835
Bst6I CTCTTC 2 cut(s) 411, 1757
BstC8I GCNNGC 6 cut(s) 60, 123, 340, 669, 1261, 1590
BstDEI CTNAG 6 cut(s) 390, 631, 650, 1205, 1342, 1810
BstDSI CCRYGG 2 cut(s) 343, 1193
BstEII GGTNACC 1 cut(s) 298
BstENI CCTNNNNNAGG 1 cut(s) 50
BstF5I GGATG 2 cut(s) 1204, 1464
BstFNI CGCG 2 cut(s) 340, 345
BstH2I RGCGCY 2 cut(s) 362, 479
BstHHI GCGC 6 cut(s) 64, 309, 340, 342, 361, 478
BstKTI GATC 6 cut(s) 268, 404, 847, 949, 1414, 1572
BstMAI GTCTC 4 cut(s) 237, 464, 1071, 1532
BstMBI GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
BstMWI GCNNNNNNNGC 8 cut(s) 64, 103, 950, 1172, 1306, 1376, 1385, 1499
BstNI CCWGG 7 cut(s) 47, 78, 128, 332, 623, 1006, 1404
BstNSI RCATGY 2 cut(s) 815, 998
BstPI GGTNACC 1 cut(s) 298
BstSFI CTRYAG 3 cut(s) 362, 693, 1310
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 2 cut(s) 340, 345
BstV2I GAAGAC 1 cut(s) 1742
BstX2I RGATCY 3 cut(s) 401, 844, 946
BstYI RGATCY 3 cut(s) 401, 844, 946
BsuRI GGCC 4 cut(s) 121, 125, 152, 1001
BtgI CCRYGG 2 cut(s) 343, 1193
BtsCI GGATG 2 cut(s) 1204, 1464
BtsI GCAGTG 1 cut(s) 922
BtsIMutI CAGTG 3 cut(s) 922, 1356, 1484
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 6 cut(s) 60, 123, 340, 669, 1261, 1590
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 6 cut(s) 64, 309, 340, 342, 361, 478
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 3 cut(s) 43, 124, 151
Cfr42I CCGCGG 1 cut(s) 346
Cfr9I CCCGGG 1 cut(s) 643
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 1514, 1713
CviAII CATG 8 cut(s) 26, 535, 638, 812, 961, 995, 1049, 1717
CviQI GTAC 2 cut(s) 1514, 1713
DdeI CTNAG 6 cut(s) 390, 631, 650, 1205, 1342, 1810
DpnI GATC 6 cut(s) 267, 403, 846, 948, 1413, 1571
DpnII GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
DraI TTTAAA 1 cut(s) 1456
EaeI YGGCCR 1 cut(s) 999
Eam1104I CTCTTC 2 cut(s) 411, 1757
EarI CTCTTC 2 cut(s) 411, 1757
EciI GGCGGA 3 cut(s) 440, 506, 1562
Ecl136II GAGCTC 2 cut(s) 83, 1300
Eco24I GRGCYC 2 cut(s) 85, 1302
Eco31I GGTCTC 1 cut(s) 1071
Eco47I GGWCC 1 cut(s) 43
Eco47III AGCGCT 1 cut(s) 360
Eco53kI GAGCTC 2 cut(s) 83, 1300
Eco57I CTGAAG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 643
Eco91I GGTNACC 1 cut(s) 298
EcoICRI GAGCTC 2 cut(s) 83, 1300
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoO109I RGGNCCY 2 cut(s) 43, 151
EcoO65I GGTNACC 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 7 cut(s) 45, 76, 126, 330, 621, 1004, 1402
EcoT22I ATGCAT 2 cut(s) 583, 787
EcoT38I GRGCYC 2 cut(s) 85, 1302
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 8 cut(s) 29, 538, 641, 815, 964, 998, 1052, 1720
FalI AAGNNNNNCTT 2 cut(s) 1133, 1165
FaqI GGGAC 2 cut(s) 56, 1701
FatI CATG 8 cut(s) 25, 534, 637, 811, 960, 994, 1048, 1716
FauI CCCGC 2 cut(s) 338, 1268
FauNDI CATATG 1 cut(s) 894
FbaI TGATCA 2 cut(s) 265, 1569
FokI GGATG 2 cut(s) 1211, 1451
FriOI GRGCYC 2 cut(s) 85, 1302
FseI GGCCGGCC 1 cut(s) 125
FspBI CTAG 2 cut(s) 374, 854
GlaI GCGC 6 cut(s) 63, 308, 339, 341, 360, 477
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 2 cut(s) 362, 479
HaeIII GGCC 4 cut(s) 121, 125, 152, 1001
HgaI GACGC 1 cut(s) 395
HhaI GCGC 6 cut(s) 64, 309, 340, 342, 361, 478
Hin1II CATG 8 cut(s) 29, 538, 641, 815, 964, 998, 1052, 1720
Hin6I GCGC 6 cut(s) 62, 307, 338, 340, 359, 476
HinP1I GCGC 6 cut(s) 62, 307, 338, 340, 359, 476
HphI GGTGA 7 cut(s) 47, 310, 320, 466, 1421, 1513, 1744
Hpy166II GTNNAC 1 cut(s) 1831
Hpy188I TCNGA 8 cut(s) 91, 393, 406, 1123, 1172, 1813, 1819, 1825
Hpy188III TCNNGA 4 cut(s) 968, 1049, 1303, 1415
Hpy8I GTNNAC 1 cut(s) 1831
Hpy99I CGWCG 3 cut(s) 103, 106, 118
HpyAV CCTTC 5 cut(s) 32, 46, 834, 1100, 1313
HpyCH4III ACNGT 8 cut(s) 661, 1216, 1351, 1375, 1488, 1609, 1678, 1835
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 103, 950, 1172, 1306, 1376, 1385, 1499
HpyF3I CTNAG 6 cut(s) 390, 631, 650, 1205, 1342, 1810
Hsp92II CATG 8 cut(s) 29, 538, 641, 815, 964, 998, 1052, 1720
HspAI GCGC 6 cut(s) 62, 307, 338, 340, 359, 476
Kpn2I TCCGGA 1 cut(s) 1302
KpnI GGTACC 1 cut(s) 1716
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Ksp22I TGATCA 2 cut(s) 265, 1569
KspI CCGCGG 1 cut(s) 346
Kzo9I GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
LmnI GCTCC 6 cut(s) 80, 91, 322, 493, 520, 1305
LweI GCATC 6 cut(s) 511, 568, 799, 931, 1502, 1813
MaeI CTAG 2 cut(s) 374, 854
MaeIII GTNAC 5 cut(s) 298, 1216, 1279, 1345, 1672
MalI GATC 6 cut(s) 267, 403, 846, 948, 1413, 1571
MboI GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
MflI RGATCY 3 cut(s) 401, 844, 946
MhlI GDGCHC 4 cut(s) 85, 609, 630, 1302
MlsI TGGCCA 1 cut(s) 1001
MluNI TGGCCA 1 cut(s) 1001
MlyI GAGTC 3 cut(s) 1147, 1412, 1823
MmeI TCCRAC 1 cut(s) 69
Mox20I TGGCCA 1 cut(s) 1001
Mph1103I ATGCAT 2 cut(s) 583, 787
MroI TCCGGA 1 cut(s) 1302
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 1110
MscI TGGCCA 1 cut(s) 1001
MseI TTAA 5 cut(s) 723, 1140, 1185, 1227, 1455
MslI CAYNNNNRTG 1 cut(s) 1058
Msp20I TGGCCA 1 cut(s) 1001
MspA1I CMGCKG 2 cut(s) 345, 1430
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 7 cut(s) 47, 78, 128, 332, 623, 1006, 1404
MvnI CGCG 2 cut(s) 340, 345
MwoI GCNNNNNNNGC 8 cut(s) 64, 103, 950, 1172, 1306, 1376, 1385, 1499
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 6 cut(s) 21, 253, 304, 644, 645, 1423
NdeI CATATG 1 cut(s) 894
NdeII GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 8 cut(s) 29, 538, 641, 815, 964, 998, 1052, 1720
NlaIV GGNNCC 9 cut(s) 18, 44, 93, 403, 608, 846, 948, 1714, 1789
NmuCI GTSAC 4 cut(s) 298, 1216, 1345, 1672
NsiI ATGCAT 2 cut(s) 583, 787
NspI RCATGY 2 cut(s) 815, 998
OliI CACNNNNGTG 1 cut(s) 1058
PagI TCATGA 1 cut(s) 1048
PaqCI CACCTGC 1 cut(s) 286
PauI GCGCGC 1 cut(s) 338
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 110
PctI GAATGC 1 cut(s) 787
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 8 cut(s) 6, 29, 370, 562, 743, 1323, 1747, 1820
PfoI TCCNGGA 1 cut(s) 251
PleI GAGTC 3 cut(s) 1147, 1412, 1822
PpsI GAGTC 3 cut(s) 1147, 1412, 1822
PpuMI RGGWCCY 1 cut(s) 43
PsiI TTATAA 1 cut(s) 1694
Psp124BI GAGCTC 2 cut(s) 85, 1302
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 7 cut(s) 45, 76, 126, 330, 621, 1004, 1402
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 7 cut(s) 45, 76, 126, 330, 621, 1004, 1402
PspN4I GGNNCC 9 cut(s) 18, 44, 93, 403, 608, 846, 948, 1714, 1789
PspPI GGNCC 3 cut(s) 43, 124, 151
PspPPI RGGWCCY 1 cut(s) 43
PstI CTGCAG 2 cut(s) 366, 697
PsuI RGATCY 3 cut(s) 401, 844, 946
PteI GCGCGC 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 1430
RigI GGCCGGCC 1 cut(s) 125
RsaI GTAC 2 cut(s) 1515, 1714
RsaNI GTAC 2 cut(s) 1514, 1713
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 2 cut(s) 85, 1302
SacII CCGCGG 1 cut(s) 346
SaqAI TTAA 5 cut(s) 723, 1140, 1185, 1227, 1455
Sau3AI GATC 6 cut(s) 265, 401, 844, 946, 1411, 1569
Sau96I GGNCC 3 cut(s) 43, 124, 151
SchI GAGTC 3 cut(s) 1147, 1412, 1823
SduI GDGCHC 4 cut(s) 85, 609, 630, 1302
SfaNI GCATC 6 cut(s) 511, 568, 799, 931, 1502, 1813
SfcI CTRYAG 3 cut(s) 362, 693, 1310
Sfr303I CCGCGG 1 cut(s) 346
SgrBI CCGCGG 1 cut(s) 346
SinI GGWCC 1 cut(s) 43
SmaI CCCGGG 1 cut(s) 645
SmiMI CAYNNNNRTG 1 cut(s) 1058
SspMI CTAG 2 cut(s) 374, 854
SstI GAGCTC 2 cut(s) 85, 1302
TaaI ACNGT 8 cut(s) 661, 1216, 1351, 1375, 1488, 1609, 1678, 1835
TaqI TCGA 5 cut(s) 71, 116, 245, 939, 1778
TauI GCSGC 5 cut(s) 97, 109, 345, 491, 1648
TfiI GAWTC 8 cut(s) 6, 29, 370, 562, 743, 1323, 1747, 1820
Tru1I TTAA 5 cut(s) 723, 1140, 1185, 1227, 1455
Tru9I TTAA 5 cut(s) 723, 1140, 1185, 1227, 1455
TscAI CASTG 3 cut(s) 922, 1356, 1491
TseFI GTSAC 4 cut(s) 298, 1216, 1345, 1672
Tsp45I GTSAC 4 cut(s) 298, 1216, 1345, 1672
TspDTI ATGAA 7 cut(s) 429, 472, 1037, 1194, 1215, 1453, 1614
TspGWI ACGGA 3 cut(s) 335, 554, 1789
TspMI CCCGGG 1 cut(s) 643
TspRI CASTG 3 cut(s) 922, 1356, 1491
VpaK11BI GGWCC 1 cut(s) 43
XagI CCTNNNNNAGG 1 cut(s) 50
XapI RAATTY 5 cut(s) 166, 193, 220, 247, 1458
XceI RCATGY 2 cut(s) 815, 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 1280
XmaI CCCGGG 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 2 cut(s) 374, 854
Zsp2I ATGCAT 2 cut(s) 583, 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.