RLG00000024470

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008293
Physical Location & Seq
Reverse (-)
36129535 .. 36132146
2612 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024470

Sequence Viewer

Length: 1830 bp
ATGCTGAATGTCCGTTTGAGAACGGGACAAGATGCAGGAGGTGAGAGGGACCTGGAGAAGGGGCTACTGGAGCCCTCAACGCCTGGAACACGATCGGAGCCGCCGATGTCACCGCACACATCGAGGCTGGCGCCGTTTTTGTCCAATTCCAGCAAGTCTCTGCTGGTTTCGAATTCCGGCAAGTCTCTGATGGTTTCGAATTCCAGCAAGTCTCTGGTTATGTCGAATTCCGGCAAGCGCTTGGATCAGAAGAAGAAGTACGTGCGGCAGGTGACCGGCCGCTTCAATGACACGGAGCTCCACCTGGCGGCGAAGCGGGGCGACGTCGCCGCCGTGAAGCATATCATCGGCGAGATTGATGCACAGATGATGGGGACCACGAGTGGAGCTGAGTTTGATGCGGAGGTGGCTGAGATTAGGTCTGCGGTTGTGAATGAGGTCAATGAGTTGGGAGAGACGCCGCTTCTCGCCGCCGCCGAGAAGGGGCACCTTGGTGTGGTGAAGGAGCTCTTGCCTTTCACCACCGATGAGGGGATTTACATGAAGAATAGGATCGGCTACAATCCATTTCATATTGCTGCTAGTCAAGGGCACCATGCCATTATCCAGGTCCTGTTAGACCATGAACCAGGACTTAGCAAAACGCTTGGCCCGTCAAATGCAACAGCTCTTGTGGCTGCAGCTACAAGAGGGCATACAGATGTGGTTAAGGAGTTAATGAATCATGATCCTAGCTTGGTAGAAATTTCTAAATCCAATGGTAAGAATGCATTGCATTTTGCGGCACGGCAGGGCCATGTAGATATTGTGAAAGCTTTGCTTGACAAGGATCCACAACTAGTAAGAAGAATTGACAAGAAAGGACAGACTGCTTTGCATATGGCCGTAAAAGGTCATAGCTGTGAGGTGGTGAAGTTGCTACTTGACACAGATTCAGCTCTTGTCATGCTCCCAGACAAGTTCGGCAATTTAGCATTACATGTGGCCACGAGGAAGAAGAGAACAGAGATAGTAAATGAGTTGTTAGCCCTTCGTGACACAAATGTGAATGCTTTAACAAGAGAACAGAAAACACCCCTTGATATAGTTGAAGCACTTCCTCTCTCTGAAGAATCCACGGCAATTAAGGAAAGCTTAACTGAGTATGGTGGTCTCCGAGCCCAGGACCTTAACCGGCCAAGAGATGAGCTTAGAAACACAGTGTCAGCTATAAAAAAAGATGTCCACACTCAGCTGGATCAAGCCCGCAAAACCAGTCGGAGTGTTACTGGAATTGCCAAGGAGATCCGTAGGCTACAAAGGGAAGGAGTCAATATTGCAACTAACTCTGTCACAGTGGTTGCATCGCTCATCGCAACAATAGCATTTGCAGCTATATTTACTGTACCTGGTGGTGATTTAGATTCTGGGAAGTCTGTGGCGGTTTCAACTACATCGTTCAAGATGTTTTATTTTTTTAATGCTATTTCACTCTTCGTAGCATTGGCTGTTGTCGTGGTACAGATCACCCTTATTCGGGGGGAGACGAAGACTGAGAGGAGGGTTATAACAGTGATTAATAAGGCGATGTGGTTGGCCTCTATGACCACTACAGTAGCATTTATTTCTGCAGCATATATAGTGGTTGGTCGACATAATCGGTGGGCTGCAATCTTTATCACAGTTATAGGAGGGATTATAATTTCAGCCGTTCTTTGTATCATGACATATTTTGTGATAAAGTCCAAAAAGATTCGTAGACTGAGAAAGAGAGAGAAGCTTCTTACAAAGACTGGATCCTCCTTGCACTCAGACTCTGGTTCAGAAGTGAATCGTGTTATGGCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

610

Amino Acids

66.29

Weight (kDa)

9.69

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 100 - 209 1.1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 155 - 206 4.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 219 - 278 2.4e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 225 - 274 4.7e-08 Ankyrin repeats (many copies)
Ank_4 PF13637 258 - 308 2e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 273 - 308 9.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 284 - 350 6.9e-10 Ankyrin repeats (3 copies)
PGG PF13962 437 - 541 1.2e-21 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1547, 1679
AarI CACCTGC 1 cut(s) 259
AatII GACGTC 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 3 cut(s) 130, 486, 591
AccI GTMKAC 2 cut(s) 1630, 1738
AclWI GGATC 9 cut(s) 252, 560, 722, 824, 837, 1245, 1279, 1770, 1783
AcoI YGGCCR 4 cut(s) 277, 882, 984, 1175
AcsI RAATTY 4 cut(s) 172, 199, 226, 744
AcuI CTGAAG 1 cut(s) 1128
AcyI GRCGYC 3 cut(s) 131, 324, 458
AfaI GTAC 3 cut(s) 260, 1386, 1500
AfeI AGCGCT 1 cut(s) 239
AfiI CCNNNNNNNGG 8 cut(s) 58, 307, 483, 496, 531, 1162, 1515, 1516
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 4 cut(s) 286, 1091, 1428, 1441
AhlI ACTAGT 1 cut(s) 838
AjnI CCWGG 7 cut(s) 51, 82, 303, 606, 628, 1161, 1387
AleI CACNNNNGTG 2 cut(s) 492, 1043
Alw21I GWGCWC 2 cut(s) 300, 510
Alw26I GTCTC 6 cut(s) 162, 189, 216, 449, 1157, 1517
AlwI GGATC 9 cut(s) 252, 560, 722, 824, 837, 1245, 1279, 1770, 1783
AlwNI CAGNNNCTG 2 cut(s) 613, 1796
Aor51HI AGCGCT 1 cut(s) 239
AoxI GGCC 8 cut(s) 277, 649, 793, 882, 984, 1175, 1575, 1821
ApeKI GCWGC 6 cut(s) 578, 677, 680, 1370, 1610, 1646
ApoI RAATTY 4 cut(s) 172, 199, 226, 744
AseI ATTAAT 1 cut(s) 1557
Asp700I GAANNNNTTC 1 cut(s) 1095
AspLEI GCGC 2 cut(s) 133, 240
AspS9I GGNCC 7 cut(s) 49, 375, 610, 650, 793, 1165, 1822
AsuHPI GGTGA 8 cut(s) 53, 102, 283, 511, 511, 922, 1406, 1498
AsuII TTCGAA 2 cut(s) 170, 197
AvaII GGWCC 4 cut(s) 49, 375, 610, 1165
BaeGI GKGCMC 2 cut(s) 489, 594
BalI TGGCCA 1 cut(s) 986
BamHI GGATCC 2 cut(s) 829, 1775
BanI GGYRCC 3 cut(s) 130, 486, 591
BanII GRGCYC 4 cut(s) 75, 300, 510, 1162
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BauI CACGAG 2 cut(s) 379, 988
BbsI GAAGAC 1 cut(s) 1535
Bbv12I GWGCWC 2 cut(s) 300, 510
BbvI GCAGC 6 cut(s) 565, 664, 692, 1382, 1622, 1633
BccI CCATC 2 cut(s) 184, 364
BceAI ACGGC 6 cut(s) 118, 317, 803, 869, 1134, 1673
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
BcoDI GTCTC 6 cut(s) 162, 189, 216, 449, 1157, 1517
BcuI ACTAGT 1 cut(s) 838
BfaI CTAG 3 cut(s) 582, 732, 839
BfmI CTRYAG 3 cut(s) 678, 1590, 1608
BfoI RGCGCY 2 cut(s) 134, 241
BfuAI ACCTGC 1 cut(s) 259
Bme1390I CCNGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
Bme18I GGWCC 4 cut(s) 49, 375, 610, 1165
BmgT120I GGNCC 7 cut(s) 49, 375, 610, 650, 793, 1165, 1822
BmiI GGNNCC 9 cut(s) 50, 72, 99, 132, 376, 488, 593, 831, 1777
BmrFI CCNGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
BmsI GCATC 4 cut(s) 22, 349, 388, 1352
BpiI GAAGAC 1 cut(s) 1535
BpmI CTGGAG 2 cut(s) 74, 89
Bpu14I TTCGAA 2 cut(s) 170, 197
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 3 cut(s) 131, 324, 458
BsaI GGTCTC 1 cut(s) 1157
BsaJI CCNNGG 4 cut(s) 490, 1116, 1161, 1278
Bsc4I CCNNNNNNNGG 8 cut(s) 58, 307, 483, 496, 531, 1162, 1515, 1516
Bse118I RCCGGY 2 cut(s) 275, 1173
Bse1I ACTGG 4 cut(s) 72, 1254, 1273, 1777
Bse3DI GCAATG 1 cut(s) 770
BseBI CCWGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
BseDI CCNNGG 4 cut(s) 490, 1116, 1161, 1278
BseLI CCNNNNNNNGG 8 cut(s) 58, 307, 483, 496, 531, 1162, 1515, 1516
BseMI GCAATG 1 cut(s) 770
BseMII CTCAG 7 cut(s) 381, 402, 1131, 1244, 1524, 1733, 1803
BseNI ACTGG 4 cut(s) 72, 1254, 1273, 1777
BseRI GAGGAG 1 cut(s) 1552
BseSI GKGCMC 2 cut(s) 489, 594
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 6 cut(s) 565, 664, 692, 1382, 1622, 1633
Bsh1285I CGRYCG 2 cut(s) 95, 280
BshFI GGCC 8 cut(s) 279, 651, 795, 884, 986, 1177, 1577, 1823
BshNI GGYRCC 3 cut(s) 130, 486, 591
BsiEI CGRYCG 2 cut(s) 95, 280
BsiHKAI GWGCWC 2 cut(s) 300, 510
BsiSI CCGG 4 cut(s) 177, 231, 276, 1174
BslFI GGGAC 3 cut(s) 39, 62, 388
BslI CCNNNNNNNGG 8 cut(s) 58, 307, 483, 496, 531, 1162, 1515, 1516
BsmAI GTCTC 6 cut(s) 162, 189, 216, 449, 1157, 1517
BsmBI CGTCTC 2 cut(s) 449, 1517
BsmFI GGGAC 3 cut(s) 39, 62, 388
BsmI GAATGC 2 cut(s) 772, 1054
BsnI GGCC 8 cut(s) 279, 651, 795, 884, 986, 1177, 1577, 1823
Bso31I GGTCTC 1 cut(s) 1157
Bsp119I TTCGAA 2 cut(s) 170, 197
Bsp1286I GDGCHC 6 cut(s) 75, 300, 489, 510, 594, 1162
Bsp143I GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
BspANI GGCC 8 cut(s) 279, 651, 795, 884, 986, 1177, 1577, 1823
BspCNI CTCAG 7 cut(s) 382, 403, 1132, 1243, 1525, 1734, 1802
BspHI TCATGA 2 cut(s) 724, 1701
BspLI GGNNCC 9 cut(s) 50, 72, 99, 132, 376, 488, 593, 831, 1777
BspMAI CTGCAG 2 cut(s) 682, 1612
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 9 cut(s) 252, 560, 722, 824, 837, 1245, 1279, 1770, 1783
BspT104I TTCGAA 2 cut(s) 170, 197
BspT107I GGYRCC 3 cut(s) 130, 486, 591
BspTNI GGTCTC 1 cut(s) 1157
BsrDI GCAATG 1 cut(s) 770
BsrFI RCCGGY 2 cut(s) 275, 1173
BsrI ACTGG 4 cut(s) 72, 1254, 1273, 1777
BssAI RCCGGY 2 cut(s) 275, 1173
BssECI CCNNGG 4 cut(s) 490, 1116, 1161, 1278
BssMI GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
BssNI GRCGYC 3 cut(s) 131, 324, 458
BssSI CACGAG 2 cut(s) 379, 988
BssT1I CCWWGG 2 cut(s) 490, 1278
Bst2BI CACGAG 2 cut(s) 379, 988
Bst2UI CCWGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
Bst4CI ACNGT 6 cut(s) 1201, 1336, 1384, 1552, 1594, 1663
Bst6I CTCTTC 2 cut(s) 992, 1478
BstACI GRCGYC 3 cut(s) 131, 324, 458
BstBAI YACGTR 1 cut(s) 262
BstBI TTCGAA 2 cut(s) 170, 197
BstC8I GCNNGC 3 cut(s) 129, 236, 1246
BstDEI CTNAG 9 cut(s) 390, 411, 635, 1140, 1190, 1230, 1533, 1742, 1789
BstDSI CCRYGG 1 cut(s) 1116
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 56
BstH2I RGCGCY 2 cut(s) 134, 241
BstHHI GCGC 2 cut(s) 133, 240
BstKTI GATC 9 cut(s) 95, 247, 555, 730, 832, 1240, 1287, 1506, 1778
BstMAI GTCTC 6 cut(s) 162, 189, 216, 449, 1157, 1517
BstMBI GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
BstMCI CGRYCG 2 cut(s) 95, 280
BstMWI GCNNNNNNNGC 6 cut(s) 70, 79, 407, 674, 1361, 1370
BstNI CCWGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
BstNSI RCATGY 1 cut(s) 983
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 7 cut(s) 51, 82, 303, 606, 628, 1161, 1387
BstSFI CTRYAG 3 cut(s) 678, 1590, 1608
BstSLI GKGCMC 2 cut(s) 489, 594
BstV1I GCAGC 6 cut(s) 565, 664, 692, 1382, 1622, 1633
BstV2I GAAGAC 1 cut(s) 1535
BstX2I RGATCY 3 cut(s) 829, 1284, 1775
BstYI RGATCY 3 cut(s) 829, 1284, 1775
BstZI CGGCCG 1 cut(s) 277
BsuRI GGCC 8 cut(s) 279, 651, 795, 884, 986, 1177, 1577, 1823
BtgI CCRYGG 1 cut(s) 1116
BtgZI GCGATG 3 cut(s) 1329, 1336, 1580
BtsIMutI CAGTG 3 cut(s) 1206, 1341, 1557
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 3 cut(s) 129, 236, 1246
CaiI CAGNNNCTG 2 cut(s) 613, 1796
CciI TCATGA 2 cut(s) 724, 1701
CfoI GCGC 2 cut(s) 133, 240
Cfr10I RCCGGY 2 cut(s) 275, 1173
Cfr13I GGNCC 7 cut(s) 49, 375, 610, 650, 793, 1165, 1822
CseI GACGC 1 cut(s) 466
CsiI ACCWGGT 1 cut(s) 1387
Csp6I GTAC 3 cut(s) 259, 1385, 1499
CviAII CATG 8 cut(s) 541, 596, 623, 725, 797, 946, 980, 1702
CviQI GTAC 3 cut(s) 259, 1385, 1499
DdeI CTNAG 9 cut(s) 390, 411, 635, 1140, 1190, 1230, 1533, 1742, 1789
DinI GGCGCC 1 cut(s) 132
DpnI GATC 9 cut(s) 94, 246, 554, 729, 831, 1239, 1286, 1505, 1777
DpnII GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
EaeI YGGCCR 4 cut(s) 277, 882, 984, 1175
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 992, 1478
EarI CTCTTC 2 cut(s) 992, 1478
Ecl136II GAGCTC 2 cut(s) 298, 508
EclXI CGGCCG 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 490, 1278
Eco24I GRGCYC 4 cut(s) 75, 300, 510, 1162
Eco31I GGTCTC 1 cut(s) 1157
Eco47I GGWCC 4 cut(s) 49, 375, 610, 1165
Eco47III AGCGCT 1 cut(s) 239
Eco52I CGGCCG 1 cut(s) 277
Eco53kI GAGCTC 2 cut(s) 298, 508
Eco57I CTGAAG 1 cut(s) 1128
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 2 cut(s) 298, 508
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoO109I RGGNCCY 3 cut(s) 49, 610, 1165
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 3 cut(s) 172, 199, 226
EcoRII CCWGG 7 cut(s) 51, 82, 303, 606, 628, 1161, 1387
EcoT14I CCWWGG 2 cut(s) 490, 1278
EcoT22I ATGCAT 1 cut(s) 772
EcoT38I GRGCYC 4 cut(s) 75, 300, 510, 1162
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 490, 1278
Esp3I CGTCTC 2 cut(s) 449, 1517
FaeI CATG 8 cut(s) 544, 599, 626, 728, 800, 949, 983, 1705
FalI AAGNNNNNCTT 6 cut(s) 494, 526, 804, 836, 1118, 1150
FaqI GGGAC 3 cut(s) 39, 62, 388
FatI CATG 8 cut(s) 540, 595, 622, 724, 796, 945, 979, 1701
FauI CCCGC 2 cut(s) 309, 1253
FauNDI CATATG 1 cut(s) 879
FblI GTMKAC 2 cut(s) 1630, 1738
FriOI GRGCYC 4 cut(s) 75, 300, 510, 1162
FspBI CTAG 3 cut(s) 582, 732, 839
GlaI GCGC 2 cut(s) 132, 239
GsuI CTGGAG 2 cut(s) 74, 89
HaeII RGCGCY 2 cut(s) 134, 241
HaeIII GGCC 8 cut(s) 279, 651, 795, 884, 986, 1177, 1577, 1823
HapII CCGG 4 cut(s) 177, 231, 276, 1174
HgaI GACGC 1 cut(s) 466
HhaI GCGC 2 cut(s) 133, 240
Hin1I GRCGYC 3 cut(s) 131, 324, 458
Hin1II CATG 8 cut(s) 544, 599, 626, 728, 800, 949, 983, 1705
Hin6I GCGC 2 cut(s) 131, 238
HinP1I GCGC 2 cut(s) 131, 238
HincII GTYRAC 1 cut(s) 1631
HindII GTYRAC 1 cut(s) 1631
HindIII AAGCTT 3 cut(s) 813, 1132, 1757
HinfI GANTC 8 cut(s) 721, 932, 1112, 1308, 1403, 1732, 1793, 1810
HpaII CCGG 4 cut(s) 177, 231, 276, 1174
HphI GGTGA 8 cut(s) 53, 102, 283, 511, 511, 922, 1406, 1498
Hpy166II GTNNAC 3 cut(s) 1225, 1631, 1739
Hpy188I TCNGA 8 cut(s) 97, 189, 249, 1108, 1157, 1260, 1792, 1804
Hpy188III TCNNGA 4 cut(s) 725, 1034, 1441, 1702
Hpy8I GTNNAC 3 cut(s) 1225, 1631, 1739
Hpy99I CGWCG 2 cut(s) 326, 329
HpyAV CCTTC 5 cut(s) 52, 475, 496, 1040, 1298
HpyCH4III ACNGT 6 cut(s) 1201, 1336, 1384, 1552, 1594, 1663
HpyCH4IV ACGT 2 cut(s) 261, 324
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 79, 407, 674, 1361, 1370
HpyF3I CTNAG 9 cut(s) 390, 411, 635, 1140, 1190, 1230, 1533, 1742, 1789
HpySE526I ACGT 2 cut(s) 261, 324
Hsp92I GRCGYC 3 cut(s) 131, 324, 458
Hsp92II CATG 8 cut(s) 544, 599, 626, 728, 800, 949, 983, 1705
HspAI GCGC 2 cut(s) 131, 238
KasI GGCGCC 1 cut(s) 130
Kzo9I GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
LmnI GCTCC 7 cut(s) 70, 97, 295, 303, 386, 505, 954
Lsp1109I GCAGC 6 cut(s) 565, 664, 692, 1382, 1622, 1633
LweI GCATC 4 cut(s) 22, 349, 388, 1352
MabI ACCWGGT 1 cut(s) 1387
MaeI CTAG 3 cut(s) 582, 732, 839
MaeII ACGT 2 cut(s) 261, 324
MaeIII GTNAC 5 cut(s) 108, 271, 1034, 1264, 1330
MalI GATC 9 cut(s) 94, 246, 554, 729, 831, 1239, 1286, 1505, 1777
MboI GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
MboII GAAGA 9 cut(s) 262, 265, 556, 858, 1006, 1009, 1121, 1465, 1540
MflI RGATCY 3 cut(s) 829, 1284, 1775
MhlI GDGCHC 6 cut(s) 75, 300, 489, 510, 594, 1162
MlsI TGGCCA 1 cut(s) 986
MluNI TGGCCA 1 cut(s) 986
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 2 cut(s) 1317, 1787
MmeI TCCRAC 1 cut(s) 1238
Mox20I TGGCCA 1 cut(s) 986
Mph1103I ATGCAT 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 1095
MscI TGGCCA 1 cut(s) 986
MseI TTAA 9 cut(s) 708, 716, 1055, 1125, 1136, 1170, 1458, 1557, 1828
MslI CAYNNNNRTG 4 cut(s) 492, 699, 900, 1043
Msp20I TGGCCA 1 cut(s) 986
MspA1I CMGCKG 1 cut(s) 1234
MspI CCGG 4 cut(s) 177, 231, 276, 1174
MspR9I CCNGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
Mva1269I GAATGC 2 cut(s) 772, 1054
MvaI CCWGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
MwoI GCNNNNNNNGC 6 cut(s) 70, 79, 407, 674, 1361, 1370
NarI GGCGCC 1 cut(s) 131
NdeI CATATG 1 cut(s) 879
NdeII GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
NlaIII CATG 8 cut(s) 544, 599, 626, 728, 800, 949, 983, 1705
NlaIV GGNNCC 9 cut(s) 50, 72, 99, 132, 376, 488, 593, 831, 1777
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 4 cut(s) 108, 271, 1034, 1330
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 1 cut(s) 983
NspV TTCGAA 2 cut(s) 170, 197
OliI CACNNNNGTG 2 cut(s) 492, 1043
PagI TCATGA 2 cut(s) 724, 1701
PaqCI CACCTGC 1 cut(s) 259
PciI ACATGT 1 cut(s) 979
PcsI WCGNNNNNNNCGW 4 cut(s) 101, 330, 474, 650
PctI GAATGC 2 cut(s) 772, 1054
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 6 cut(s) 721, 932, 1112, 1403, 1732, 1810
Ple19I CGATCG 1 cut(s) 95
PleI GAGTC 2 cut(s) 1316, 1787
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 2 cut(s) 1316, 1787
Ppu21I YACGTR 1 cut(s) 262
PpuMI RGGWCCY 3 cut(s) 49, 610, 1165
PscI ACATGT 1 cut(s) 979
PshBI ATTAAT 1 cut(s) 1557
PsiI TTATAA 2 cut(s) 1547, 1679
Psp124BI GAGCTC 2 cut(s) 300, 510
Psp5II RGGWCCY 3 cut(s) 49, 610, 1165
Psp6I CCWGG 7 cut(s) 51, 82, 303, 606, 628, 1161, 1387
PspEI GGTNACC 1 cut(s) 271
PspGI CCWGG 7 cut(s) 51, 82, 303, 606, 628, 1161, 1387
PspN4I GGNNCC 9 cut(s) 50, 72, 99, 132, 376, 488, 593, 831, 1777
PspPI GGNCC 7 cut(s) 49, 375, 610, 650, 793, 1165, 1822
PspPPI RGGWCCY 3 cut(s) 49, 610, 1165
PstI CTGCAG 2 cut(s) 682, 1612
PstNI CAGNNNCTG 2 cut(s) 613, 1796
PsuI RGATCY 3 cut(s) 829, 1284, 1775
PvuI CGATCG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 1234
RsaI GTAC 3 cut(s) 260, 1386, 1500
RsaNI GTAC 3 cut(s) 259, 1385, 1499
RseI CAYNNNNRTG 4 cut(s) 492, 699, 900, 1043
SacI GAGCTC 2 cut(s) 300, 510
SalI GTCGAC 1 cut(s) 1629
SaqAI TTAA 9 cut(s) 708, 716, 1055, 1125, 1136, 1170, 1458, 1557, 1828
Sau3AI GATC 9 cut(s) 92, 244, 552, 727, 829, 1237, 1284, 1503, 1775
Sau96I GGNCC 7 cut(s) 49, 375, 610, 650, 793, 1165, 1822
SchI GAGTC 2 cut(s) 1317, 1787
ScrFI CCNGG 7 cut(s) 53, 84, 305, 608, 630, 1163, 1389
SduI GDGCHC 6 cut(s) 75, 300, 489, 510, 594, 1162
SexAI ACCWGGT 1 cut(s) 1387
SfaNI GCATC 4 cut(s) 22, 349, 388, 1352
SfcI CTRYAG 3 cut(s) 678, 1590, 1608
SfoI GGCGCC 1 cut(s) 132
SfuI TTCGAA 2 cut(s) 170, 197
SinI GGWCC 4 cut(s) 49, 375, 610, 1165
SmiMI CAYNNNNRTG 4 cut(s) 492, 699, 900, 1043
SpeI ACTAGT 1 cut(s) 838
SspDI GGCGCC 1 cut(s) 130
SspI AATATT 1 cut(s) 1315
SspMI CTAG 3 cut(s) 582, 732, 839
SstI GAGCTC 2 cut(s) 300, 510
StyD4I CCNGG 7 cut(s) 51, 82, 303, 606, 628, 1161, 1387
StyI CCWWGG 2 cut(s) 490, 1278
TaaI ACNGT 6 cut(s) 1201, 1336, 1384, 1552, 1594, 1663
TaiI ACGT 2 cut(s) 264, 327
TaqI TCGA 5 cut(s) 122, 170, 197, 224, 1630
TauI GCSGC 9 cut(s) 103, 268, 282, 311, 332, 463, 473, 476, 785
TfiI GAWTC 6 cut(s) 721, 932, 1112, 1403, 1732, 1810
Tru1I TTAA 9 cut(s) 708, 716, 1055, 1125, 1136, 1170, 1458, 1557, 1828
Tru9I TTAA 9 cut(s) 708, 716, 1055, 1125, 1136, 1170, 1458, 1557, 1828
TscAI CASTG 3 cut(s) 1206, 1341, 1557
TseFI GTSAC 4 cut(s) 108, 271, 1034, 1330
TseI GCWGC 6 cut(s) 578, 677, 680, 1370, 1610, 1646
Tsp45I GTSAC 4 cut(s) 108, 271, 1034, 1330
TspDTI ATGAA 4 cut(s) 557, 560, 639, 734
TspGWI ACGGA 2 cut(s) 308, 1277
TspRI CASTG 3 cut(s) 1206, 1341, 1557
VpaK11BI GGWCC 4 cut(s) 49, 375, 610, 1165
VspI ATTAAT 1 cut(s) 1557
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 4 cut(s) 172, 199, 226, 744
XceI RCATGY 1 cut(s) 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmiI GTMKAC 2 cut(s) 1630, 1738
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 3 cut(s) 582, 732, 839
ZraI GACGTC 1 cut(s) 325
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.