Rroxscaffold_6G00413350

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008293
Physical Location & Seq
Reverse (-)
35725191 .. 35727834
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00413350.1

Sequence Viewer

Length: 1800 bp
ATGCTGAATGTCCGTTTGAGAACGGGACAAGATGCAGACGATGATGATGTGTTAGCAGGAGGTGAGAGGGACCTGGAGAAGGGGCTACTGGAGCCCTCAACGCCGGGAACTCGATCGGAGCCGCCGATGTCACCGCACACATCAAGGCTGGTGCCGTTTTTGTCCAATTCCAGCAAGTCTCTGCTGGTTTCGAATTCTAGCAAGTCTCTGCTGGTTTCGAATTCCGGCAAGTCTCTGATGGTTTCGAATTCCAGCAAGTCTCTGGTATTGTCGAATTCCGGCAACCGCTTGGATCAGAAGAAGATGTACGTGCGGCAGGTGACCGGCCGCTTCCATGACACGGAGCTCCACCTGGCGGCGAAGCGGGGCGACGTCGCAGCCGTGAAGCATATCATCGGCGAGATTGATGCACAGATGATGGGGACCACGAGTGGGGCTGACTTTGATGCGGAGGTGGCTGAGATTAGGTCTGCGGTTGTGAATGAGGTCAATGAGTTGGGAGAGACGCCGCTTCTCGCCGCCGCCGAGAAGGGGCACCTTGGTGTGGTGAAGGAGCTCTTGCCTTTCACCACCGATGAGGGGATTTACATGAAGAATAGGATCGGCTACAATCCATTGCATATTGCTGCTAGTCAAGGGCACCATGCCATTATCCAGGTCCTGTTAGACCATGAACCAGGACTTAGCAAAACACTTGGCCCGTCAAATGCAACAGCTCTTGTGGCTGCAGCTACAAGAGGGCATACAGATGTGGTTAAGGAGTTAATGAATCATGATCCTAACTTGGTAGAAATTTCTAAATCCAATGGTAAGAATGCATTGCATTTTGCGGCACGGCAGGGCCATGTAGATATTGTGAAAGCTTTGCTTGACAAGGATCCACAACTAGTAAGAAGAATTGACAAGAAAGGACAGACTGCTTTGCATATGGCCGTAAAAGGTCATAGCTGTGAGGTGGTGAAGTTGCTACTTGACACAGATTCAGCTCTTGTCATGCTCCCAGACAAGTTCGGCAATTTACCATTACATGTGGCCACGAGGAAGAAGAGAACAGAGATAGTCAATGAGCTGTTAGCCCTTCGTGACACAAATGTGAATGCTTTAACAAGAGAACAGAAAACACCCCTTGATATAGACCTTAACCGGCCAAGAGATGAGCTTAGAAACACTGTGTCAGCTATAAAAAAAGATGTCCACACTCAGCTGGATCAAGCCCGCAAAACCAGTCGGAGTGTTACTGGAATTGCCAAGGAGATCCGTAGGCTACAAAGGGAAGGAGTCAATATTGCAACTAACTCTGTCACAGTGGTTGCATCGCTCATCGCAACAATAGCATTTGCAGCTATATTTACTGTACCTGGTGGTGATTTAGATTCTGGGAAGTCTGTGGCGGTTTCAACTACATCGTTCAAGATGTTTTATTTTTTTAATGCTATTTCACTCTTCGTAGCATTGGCTGTTGTCGTGGTACAGATCACCCTTATTCGGGGGGAAACGAAGACTGAGAGGAGGGTTATAACAGTGATTAATAAGGCGATGTGGTTGGCCTCTATGACCACTACAGTAGCATTTATTTCTGCAGCATATATAGTGGTTGGTCGACATAATCGGTGGGCTGCAATCTTTATCACAGTTATAGGAGGGATTATAATTTCAGCCGTTCTTTGTATCATGACATATTTTGTGATAAAGTCCAAAAAGATTCGTAGACTGAGAAAGAGAGAGAAGCTTCTTACAAGGACTGGATCCTCCTTGCACTCAGACTCTGGTTCAGAAGTGAATCGTGTTATGGCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

599

Amino Acids

65.23

Weight (kDa)

9.66

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 116 - 225 8.9e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 171 - 222 1.7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 235 - 294 1.5e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 241 - 290 2.1e-08 Ankyrin repeats (many copies)
Ank_4 PF13637 274 - 324 2e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 289 - 324 9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 301 - 366 5.2e-10 Ankyrin repeats (3 copies)
PGG PF13962 427 - 531 1.1e-21 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1517, 1649
AarI CACCTGC 1 cut(s) 307
AatII GACGTC 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 3 cut(s) 151, 534, 639
AccI GTMKAC 2 cut(s) 1600, 1708
AclWI GGATC 9 cut(s) 300, 608, 770, 872, 885, 1215, 1249, 1740, 1753
AcoI YGGCCR 4 cut(s) 325, 930, 1032, 1145
AcsI RAATTY 5 cut(s) 193, 220, 247, 274, 792
AcyI GRCGYC 2 cut(s) 372, 506
AfaI GTAC 3 cut(s) 308, 1356, 1470
AfiI CCNNNNNNNGG 9 cut(s) 79, 340, 355, 432, 531, 544, 579, 1485, 1486
AflIII ACRYGT 1 cut(s) 1027
AgsI TTSAA 2 cut(s) 1398, 1411
AhlI ACTAGT 1 cut(s) 886
AjnI CCWGG 5 cut(s) 72, 351, 654, 676, 1357
AleI CACNNNNGTG 2 cut(s) 540, 1091
Alw21I GWGCWC 2 cut(s) 348, 558
Alw26I GTCTC 5 cut(s) 183, 210, 237, 264, 497
AlwI GGATC 9 cut(s) 300, 608, 770, 872, 885, 1215, 1249, 1740, 1753
AlwNI CAGNNNCTG 2 cut(s) 661, 1766
AoxI GGCC 8 cut(s) 325, 697, 841, 930, 1032, 1145, 1545, 1791
ApeKI GCWGC 7 cut(s) 377, 626, 725, 728, 1340, 1580, 1616
ApoI RAATTY 5 cut(s) 193, 220, 247, 274, 792
AseI ATTAAT 1 cut(s) 1527
AspS9I GGNCC 6 cut(s) 70, 423, 658, 698, 841, 1792
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 8 cut(s) 74, 123, 331, 559, 559, 970, 1376, 1468
AsuII TTCGAA 3 cut(s) 191, 218, 245
AvaII GGWCC 3 cut(s) 70, 423, 658
BaeGI GKGCMC 2 cut(s) 537, 642
BalI TGGCCA 1 cut(s) 1034
BamHI GGATCC 2 cut(s) 877, 1745
BanI GGYRCC 3 cut(s) 151, 534, 639
BanII GRGCYC 3 cut(s) 96, 348, 558
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
BauI CACGAG 2 cut(s) 427, 1036
BbsI GAAGAC 1 cut(s) 1505
Bbv12I GWGCWC 2 cut(s) 348, 558
BbvI GCAGC 7 cut(s) 389, 613, 712, 740, 1352, 1592, 1603
BccI CCATC 2 cut(s) 232, 412
BceAI ACGGC 5 cut(s) 139, 365, 851, 917, 1643
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 5 cut(s) 74, 353, 656, 678, 1359
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 5 cut(s) 183, 210, 237, 264, 497
BcuI ACTAGT 1 cut(s) 886
BfaI CTAG 3 cut(s) 198, 630, 887
BfmI CTRYAG 3 cut(s) 726, 1560, 1578
BfuAI ACCTGC 1 cut(s) 307
Bme1390I CCNGG 6 cut(s) 74, 105, 353, 656, 678, 1359
Bme18I GGWCC 3 cut(s) 70, 423, 658
BmgT120I GGNCC 6 cut(s) 70, 423, 658, 698, 841, 1792
BmiI GGNNCC 9 cut(s) 71, 93, 120, 153, 424, 536, 641, 879, 1747
BmrFI CCNGG 6 cut(s) 74, 105, 353, 656, 678, 1359
BmsI GCATC 4 cut(s) 22, 397, 436, 1322
BpiI GAAGAC 1 cut(s) 1505
BpmI CTGGAG 2 cut(s) 95, 110
Bpu14I TTCGAA 3 cut(s) 191, 218, 245
BpuMI CCSGG 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 310
BsaHI GRCGYC 2 cut(s) 372, 506
BsaJI CCNNGG 2 cut(s) 538, 1248
Bsc4I CCNNNNNNNGG 9 cut(s) 79, 340, 355, 432, 531, 544, 579, 1485, 1486
Bse118I RCCGGY 2 cut(s) 323, 1143
Bse1I ACTGG 4 cut(s) 93, 1224, 1243, 1747
Bse3DI GCAATG 2 cut(s) 614, 818
BseBI CCWGG 5 cut(s) 74, 353, 656, 678, 1359
BseDI CCNNGG 2 cut(s) 538, 1248
BseLI CCNNNNNNNGG 9 cut(s) 79, 340, 355, 432, 531, 544, 579, 1485, 1486
BseMI GCAATG 2 cut(s) 614, 818
BseMII CTCAG 5 cut(s) 450, 1214, 1494, 1703, 1773
BseNI ACTGG 4 cut(s) 93, 1224, 1243, 1747
BseRI GAGGAG 1 cut(s) 1522
BseSI GKGCMC 2 cut(s) 537, 642
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 7 cut(s) 389, 613, 712, 740, 1352, 1592, 1603
Bsh1285I CGRYCG 2 cut(s) 116, 328
BshFI GGCC 8 cut(s) 327, 699, 843, 932, 1034, 1147, 1547, 1793
BshNI GGYRCC 3 cut(s) 151, 534, 639
BsiEI CGRYCG 2 cut(s) 116, 328
BsiHKAI GWGCWC 2 cut(s) 348, 558
BsiSI CCGG 5 cut(s) 104, 225, 279, 324, 1144
BslFI GGGAC 3 cut(s) 39, 83, 436
BslI CCNNNNNNNGG 9 cut(s) 79, 340, 355, 432, 531, 544, 579, 1485, 1486
BsmAI GTCTC 5 cut(s) 183, 210, 237, 264, 497
BsmBI CGTCTC 1 cut(s) 497
BsmFI GGGAC 3 cut(s) 39, 83, 436
BsmI GAATGC 2 cut(s) 820, 1102
BsnI GGCC 8 cut(s) 327, 699, 843, 932, 1034, 1147, 1547, 1793
Bsp119I TTCGAA 3 cut(s) 191, 218, 245
Bsp1286I GDGCHC 5 cut(s) 96, 348, 537, 558, 642
Bsp143I GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
BspANI GGCC 8 cut(s) 327, 699, 843, 932, 1034, 1147, 1547, 1793
BspCNI CTCAG 5 cut(s) 451, 1213, 1495, 1704, 1772
BspHI TCATGA 2 cut(s) 772, 1671
BspLI GGNNCC 9 cut(s) 71, 93, 120, 153, 424, 536, 641, 879, 1747
BspMAI CTGCAG 2 cut(s) 730, 1582
BspMI ACCTGC 1 cut(s) 307
BspPI GGATC 9 cut(s) 300, 608, 770, 872, 885, 1215, 1249, 1740, 1753
BspT104I TTCGAA 3 cut(s) 191, 218, 245
BspT107I GGYRCC 3 cut(s) 151, 534, 639
BsrDI GCAATG 2 cut(s) 614, 818
BsrFI RCCGGY 2 cut(s) 323, 1143
BsrI ACTGG 4 cut(s) 93, 1224, 1243, 1747
BssAI RCCGGY 2 cut(s) 323, 1143
BssECI CCNNGG 2 cut(s) 538, 1248
BssMI GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
BssNI GRCGYC 2 cut(s) 372, 506
BssSI CACGAG 2 cut(s) 427, 1036
BssT1I CCWWGG 2 cut(s) 538, 1248
Bst2BI CACGAG 2 cut(s) 427, 1036
Bst2UI CCWGG 5 cut(s) 74, 353, 656, 678, 1359
Bst4CI ACNGT 6 cut(s) 1171, 1306, 1354, 1522, 1564, 1633
Bst6I CTCTTC 2 cut(s) 1040, 1448
BstACI GRCGYC 2 cut(s) 372, 506
BstBAI YACGTR 1 cut(s) 310
BstBI TTCGAA 3 cut(s) 191, 218, 245
BstC8I GCNNGC 1 cut(s) 1216
BstDEI CTNAG 7 cut(s) 459, 683, 1160, 1200, 1503, 1712, 1759
BstEII GGTNACC 1 cut(s) 319
BstENI CCTNNNNNAGG 1 cut(s) 77
BstKTI GATC 9 cut(s) 116, 295, 603, 778, 880, 1210, 1257, 1476, 1748
BstMAI GTCTC 5 cut(s) 183, 210, 237, 264, 497
BstMBI GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
BstMCI CGRYCG 2 cut(s) 116, 328
BstMWI GCNNNNNNNGC 6 cut(s) 91, 100, 455, 722, 1331, 1340
BstNI CCWGG 5 cut(s) 74, 353, 656, 678, 1359
BstNSI RCATGY 1 cut(s) 1031
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 6 cut(s) 72, 103, 351, 654, 676, 1357
BstSFI CTRYAG 3 cut(s) 726, 1560, 1578
BstSLI GKGCMC 2 cut(s) 537, 642
BstV1I GCAGC 7 cut(s) 389, 613, 712, 740, 1352, 1592, 1603
BstV2I GAAGAC 1 cut(s) 1505
BstX2I RGATCY 3 cut(s) 877, 1254, 1745
BstYI RGATCY 3 cut(s) 877, 1254, 1745
BstZI CGGCCG 1 cut(s) 325
BsuRI GGCC 8 cut(s) 327, 699, 843, 932, 1034, 1147, 1547, 1793
BtgZI GCGATG 3 cut(s) 1299, 1306, 1550
BtsIMutI CAGTG 3 cut(s) 1167, 1311, 1527
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 1216
CaiI CAGNNNCTG 2 cut(s) 661, 1766
CciI TCATGA 2 cut(s) 772, 1671
Cfr10I RCCGGY 2 cut(s) 323, 1143
Cfr13I GGNCC 6 cut(s) 70, 423, 658, 698, 841, 1792
CseI GACGC 1 cut(s) 514
CsiI ACCWGGT 1 cut(s) 1357
Csp6I GTAC 3 cut(s) 307, 1355, 1469
CviAII CATG 9 cut(s) 335, 589, 644, 671, 773, 845, 994, 1028, 1672
CviQI GTAC 3 cut(s) 307, 1355, 1469
DdeI CTNAG 7 cut(s) 459, 683, 1160, 1200, 1503, 1712, 1759
DpnI GATC 9 cut(s) 115, 294, 602, 777, 879, 1209, 1256, 1475, 1747
DpnII GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
EaeI YGGCCR 4 cut(s) 325, 930, 1032, 1145
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 1040, 1448
EarI CTCTTC 2 cut(s) 1040, 1448
Ecl136II GAGCTC 2 cut(s) 346, 556
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 538, 1248
Eco24I GRGCYC 3 cut(s) 96, 348, 558
Eco47I GGWCC 3 cut(s) 70, 423, 658
Eco52I CGGCCG 1 cut(s) 325
Eco53kI GAGCTC 2 cut(s) 346, 556
Eco91I GGTNACC 1 cut(s) 319
EcoICRI GAGCTC 2 cut(s) 346, 556
EcoNI CCTNNNNNAGG 1 cut(s) 77
EcoO109I RGGNCCY 2 cut(s) 70, 658
EcoO65I GGTNACC 1 cut(s) 319
EcoRI GAATTC 4 cut(s) 193, 220, 247, 274
EcoRII CCWGG 5 cut(s) 72, 351, 654, 676, 1357
EcoT14I CCWWGG 2 cut(s) 538, 1248
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 3 cut(s) 96, 348, 558
ErhI CCWWGG 2 cut(s) 538, 1248
Esp3I CGTCTC 1 cut(s) 497
FaeI CATG 9 cut(s) 338, 592, 647, 674, 776, 848, 997, 1031, 1675
FalI AAGNNNNNCTT 4 cut(s) 542, 574, 852, 884
FaqI GGGAC 3 cut(s) 39, 83, 436
FatI CATG 9 cut(s) 334, 588, 643, 670, 772, 844, 993, 1027, 1671
FauI CCCGC 2 cut(s) 357, 1223
FauNDI CATATG 1 cut(s) 927
FblI GTMKAC 2 cut(s) 1600, 1708
FriOI GRGCYC 3 cut(s) 96, 348, 558
FspBI CTAG 3 cut(s) 198, 630, 887
GsuI CTGGAG 2 cut(s) 95, 110
HaeIII GGCC 8 cut(s) 327, 699, 843, 932, 1034, 1147, 1547, 1793
HapII CCGG 5 cut(s) 104, 225, 279, 324, 1144
HgaI GACGC 1 cut(s) 514
Hin1I GRCGYC 2 cut(s) 372, 506
Hin1II CATG 9 cut(s) 338, 592, 647, 674, 776, 848, 997, 1031, 1675
HincII GTYRAC 1 cut(s) 1601
HindII GTYRAC 1 cut(s) 1601
HindIII AAGCTT 2 cut(s) 861, 1727
HinfI GANTC 7 cut(s) 769, 980, 1278, 1373, 1702, 1763, 1780
HpaII CCGG 5 cut(s) 104, 225, 279, 324, 1144
HphI GGTGA 8 cut(s) 74, 123, 331, 559, 559, 970, 1376, 1468
Hpy166II GTNNAC 3 cut(s) 1195, 1601, 1709
Hpy188I TCNGA 6 cut(s) 118, 237, 297, 1230, 1762, 1774
Hpy188III TCNNGA 4 cut(s) 773, 1082, 1411, 1672
Hpy8I GTNNAC 3 cut(s) 1195, 1601, 1709
Hpy99I CGWCG 2 cut(s) 374, 377
HpyAV CCTTC 5 cut(s) 73, 523, 544, 1088, 1268
HpyCH4III ACNGT 6 cut(s) 1171, 1306, 1354, 1522, 1564, 1633
HpyCH4IV ACGT 2 cut(s) 309, 372
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 100, 455, 722, 1331, 1340
HpyF3I CTNAG 7 cut(s) 459, 683, 1160, 1200, 1503, 1712, 1759
HpySE526I ACGT 2 cut(s) 309, 372
Hsp92I GRCGYC 2 cut(s) 372, 506
Hsp92II CATG 9 cut(s) 338, 592, 647, 674, 776, 848, 997, 1031, 1675
Kzo9I GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
LmnI GCTCC 6 cut(s) 91, 118, 343, 351, 553, 1002
Lsp1109I GCAGC 7 cut(s) 389, 613, 712, 740, 1352, 1592, 1603
LweI GCATC 4 cut(s) 22, 397, 436, 1322
MabI ACCWGGT 1 cut(s) 1357
MaeI CTAG 3 cut(s) 198, 630, 887
MaeII ACGT 2 cut(s) 309, 372
MaeIII GTNAC 5 cut(s) 129, 319, 1082, 1234, 1300
MalI GATC 9 cut(s) 115, 294, 602, 777, 879, 1209, 1256, 1475, 1747
MboI GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
MboII GAAGA 8 cut(s) 310, 313, 604, 906, 1054, 1057, 1435, 1510
MflI RGATCY 3 cut(s) 877, 1254, 1745
MhlI GDGCHC 5 cut(s) 96, 348, 537, 558, 642
MlsI TGGCCA 1 cut(s) 1034
MluNI TGGCCA 1 cut(s) 1034
MlyI GAGTC 2 cut(s) 1287, 1757
MmeI TCCRAC 1 cut(s) 1208
Mox20I TGGCCA 1 cut(s) 1034
Mph1103I ATGCAT 1 cut(s) 820
MscI TGGCCA 1 cut(s) 1034
MseI TTAA 7 cut(s) 756, 764, 1103, 1140, 1428, 1527, 1798
MslI CAYNNNNRTG 4 cut(s) 540, 747, 948, 1091
Msp20I TGGCCA 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 1204
MspI CCGG 5 cut(s) 104, 225, 279, 324, 1144
MspR9I CCNGG 6 cut(s) 74, 105, 353, 656, 678, 1359
Mva1269I GAATGC 2 cut(s) 820, 1102
MvaI CCWGG 5 cut(s) 74, 353, 656, 678, 1359
MwoI GCNNNNNNNGC 6 cut(s) 91, 100, 455, 722, 1331, 1340
NciI CCSGG 1 cut(s) 105
NdeI CATATG 1 cut(s) 927
NdeII GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
NlaIII CATG 9 cut(s) 338, 592, 647, 674, 776, 848, 997, 1031, 1675
NlaIV GGNNCC 9 cut(s) 71, 93, 120, 153, 424, 536, 641, 879, 1747
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 4 cut(s) 129, 319, 1082, 1300
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 1031
NspV TTCGAA 3 cut(s) 191, 218, 245
OliI CACNNNNGTG 2 cut(s) 540, 1091
PagI TCATGA 2 cut(s) 772, 1671
PaqCI CACCTGC 1 cut(s) 307
PciI ACATGT 1 cut(s) 1027
PcsI WCGNNNNNNNCGW 3 cut(s) 122, 378, 522
PctI GAATGC 2 cut(s) 820, 1102
PfeI GAWTC 5 cut(s) 769, 980, 1373, 1702, 1780
Ple19I CGATCG 1 cut(s) 116
PleI GAGTC 2 cut(s) 1286, 1757
PpsI GAGTC 2 cut(s) 1286, 1757
Ppu21I YACGTR 1 cut(s) 310
PpuMI RGGWCCY 2 cut(s) 70, 658
PscI ACATGT 1 cut(s) 1027
PshBI ATTAAT 1 cut(s) 1527
PsiI TTATAA 2 cut(s) 1517, 1649
Psp124BI GAGCTC 2 cut(s) 348, 558
Psp5II RGGWCCY 2 cut(s) 70, 658
Psp6I CCWGG 5 cut(s) 72, 351, 654, 676, 1357
PspEI GGTNACC 1 cut(s) 319
PspGI CCWGG 5 cut(s) 72, 351, 654, 676, 1357
PspN4I GGNNCC 9 cut(s) 71, 93, 120, 153, 424, 536, 641, 879, 1747
PspPI GGNCC 6 cut(s) 70, 423, 658, 698, 841, 1792
PspPPI RGGWCCY 2 cut(s) 70, 658
PstI CTGCAG 2 cut(s) 730, 1582
PstNI CAGNNNCTG 2 cut(s) 661, 1766
PsuI RGATCY 3 cut(s) 877, 1254, 1745
PvuI CGATCG 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 1204
RsaI GTAC 3 cut(s) 308, 1356, 1470
RsaNI GTAC 3 cut(s) 307, 1355, 1469
RseI CAYNNNNRTG 4 cut(s) 540, 747, 948, 1091
SacI GAGCTC 2 cut(s) 348, 558
SalI GTCGAC 1 cut(s) 1599
SaqAI TTAA 7 cut(s) 756, 764, 1103, 1140, 1428, 1527, 1798
Sau3AI GATC 9 cut(s) 113, 292, 600, 775, 877, 1207, 1254, 1473, 1745
Sau96I GGNCC 6 cut(s) 70, 423, 658, 698, 841, 1792
SchI GAGTC 2 cut(s) 1287, 1757
ScrFI CCNGG 6 cut(s) 74, 105, 353, 656, 678, 1359
SduI GDGCHC 5 cut(s) 96, 348, 537, 558, 642
SexAI ACCWGGT 1 cut(s) 1357
SfaNI GCATC 4 cut(s) 22, 397, 436, 1322
SfcI CTRYAG 3 cut(s) 726, 1560, 1578
SfuI TTCGAA 3 cut(s) 191, 218, 245
SinI GGWCC 3 cut(s) 70, 423, 658
SmiMI CAYNNNNRTG 4 cut(s) 540, 747, 948, 1091
SpeI ACTAGT 1 cut(s) 886
SspI AATATT 1 cut(s) 1285
SspMI CTAG 3 cut(s) 198, 630, 887
SstI GAGCTC 2 cut(s) 348, 558
StyD4I CCNGG 6 cut(s) 72, 103, 351, 654, 676, 1357
StyI CCWWGG 2 cut(s) 538, 1248
TaaI ACNGT 6 cut(s) 1171, 1306, 1354, 1522, 1564, 1633
TaiI ACGT 2 cut(s) 312, 375
TaqI TCGA 6 cut(s) 112, 191, 218, 245, 272, 1600
TauI GCSGC 8 cut(s) 124, 316, 330, 359, 511, 521, 524, 833
TfiI GAWTC 5 cut(s) 769, 980, 1373, 1702, 1780
Tru1I TTAA 7 cut(s) 756, 764, 1103, 1140, 1428, 1527, 1798
Tru9I TTAA 7 cut(s) 756, 764, 1103, 1140, 1428, 1527, 1798
TscAI CASTG 3 cut(s) 1174, 1311, 1527
TseFI GTSAC 4 cut(s) 129, 319, 1082, 1300
TseI GCWGC 7 cut(s) 377, 626, 725, 728, 1340, 1580, 1616
Tsp45I GTSAC 4 cut(s) 129, 319, 1082, 1300
TspDTI ATGAA 3 cut(s) 605, 687, 782
TspGWI ACGGA 2 cut(s) 356, 1247
TspRI CASTG 3 cut(s) 1174, 1311, 1527
VpaK11BI GGWCC 3 cut(s) 70, 423, 658
VspI ATTAAT 1 cut(s) 1527
XagI CCTNNNNNAGG 1 cut(s) 77
XapI RAATTY 5 cut(s) 193, 220, 247, 274, 792
XceI RCATGY 1 cut(s) 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 1600, 1708
XspI CTAG 3 cut(s) 198, 630, 887
ZraI GACGTC 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.