MD12G1133000.v1.1

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008293
Physical Location & Seq
Reverse (-)
20858098 .. 20860665
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1133000.v1.1.491

Sequence Viewer

Length: 1827 bp
ATGAGAATCCGTTTGGGACCGGGGCATGATTCAGGTGAGAGGGACTTGGAGAAGGGGCTGGTGCAGCCCTCAACCCCAGGAGCTCTGTCGGAGCCGCCGTCGTCACCGCACCCGTCGAGGCCCGCCCTGGTGTTCTCGAATTCCAGCAAGGCCCTGCTGGTTTCAAATTCTGGCAAGGCATTGCTGGTTTCGAATTCCAGCAAGTCTCTGGTTGCGTCTAATTCCGGGAAGCGCATTGATCAGTTGGGGAGGAAGAAGTATGTGAAGCAGGTGACTGGCCGCCACAATGACACAGAGCTCCACCTGGCTGCGCGCCGCGGGGACTTGGCAGCGGTGCATCAGTTTCTGGGGGAGATTGATGCCCAGATGGTGGGATCCGAGTTTGATGAAGAGGTGGAGGAGATGAGGTCTGCTATTGTGAATGAGGTGAATGAGTTGGGAGAGACGGCACTTTTCATAGCAGCGGAGCAGGGCCATCTTGCTATAGTGGAGGAGCTTCTGCCTTACATGACGGAGAAGGGTATTTTGTTGAAGAATCAAATTGGGTTTGATGCATTGCATATTGCTGCAAATAAAGGGCACCAAGCCGTTGTCCAGGTGCTCTTAGACCATGACCCGGGGCTTAGCAAAACAGTGGGCCAGGCAAATGCTACCCCTCTTGTGTCTGCTGCTACAAGAGGGCATACAGCCGTCGTTAAAGAATTACTATCCAAAGATTCTACTTTGCTGGAAGTAGCGAAATCCAATGGGAAGAATGCGTTGCATCTTGCTGCGCGACAGGGGCATGTGGAAATTGTGAAAACTTTGCTTGGCAGGGATCCACAACTAGCAAGAAGAATTGATAAGAAAGGACAAACTGCTCTGCATATGGCCGTAAAAGGTCATAGCAGTGAAGTTGTGAGGTTGATTGTCAATGCGGATCCAGCTATTGTCATGCTTCCAGACAAGTTCGGCAATTCAGCATTGCATGTGGCCACCAGGAAAAAGCGAGTAGATATAGTCAATGTGCTGTTGCTCGTTCGTGATACAAATGTGAATGCTCTAACAAGAGACCAGAAAACACCCCTTGATATAGCCGAAGGACTTCCATACTCTGAAGAAATCACAACAATTAAGGAAACCTTGACTCAATATGGTGCTTTCCGAGCAAATGAACTTAACCAGCCACGGGATGAGCTTAGAAACACCGTGACAGAGATACAAAAAAATGTCCATAATCAGCTTGAACAAGCCCGCAAAACCAACCAGAGTGTTACTGGAATTGCCAAGGAGCTCCGCAAGCTACAGAGGGAAGGAATCAATAATGCCACTAACTCAGTCACAGTGGTTGCTTCACTCTTTGCAACAGTAGCTTTTGCAGCTCTATTTACACTTCCTGGCGGTGATTTTGACACCGGGGCAGCTGTTGTGTCAATCCGAGTATCATTTAAAATTTTCATCCTTTCCAATGCCGTTTCACTCTTCTCATCATTGGCTGTTGTTTTGGTACAAATCACCCTCGTCAGGGGGGAGACAAAGACGGAGAGGAGGGTTGTGGAAGTGATCAATAAGTTGATGTGGCTAGCCTCTGTCTGCACTACAGTAGCATTTATATCTTCATCGTATATAGTGGTTGGGCGGCATAATTTGTGGGCTGCAATCCTTGTCACAGTTATAGGGGGACTTACAATTGGGGGAGTTATTGGTACCATGACATATTACGTGGTGAAGACCAAAAGGCTTCGCAGACTGAGGAAGAGAGAGAAGATGTTGAGGAGGAATGGAACTAACCATTCCATGCGTCACTCAGAGTCTGATTCAGAAGTGAACCGTATTTATGCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

609

Amino Acids

66.29

Weight (kDa)

9.67

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 100 - 205 8.4e-14 Ankyrin repeats (3 copies)
Ank_2 PF12796 215 - 273 1.3e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 221 - 270 1e-06 Ankyrin repeats (many copies)
Ank PF00023 249 - 274 7.8e-06 Ankyrin repeat
Ank_4 PF13637 254 - 302 1.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 280 - 346 5e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 304 - 359 2.7e-06 Ankyrin repeats (many copies)
PGG PF13962 431 - 537 2.9e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 259
Acc36I ACCTGC 1 cut(s) 259
Acc65I GGTACC 1 cut(s) 1685
AccB1I GGYRCC 2 cut(s) 579, 1685
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 3 cut(s) 313, 318, 775
AclWI GGATC 6 cut(s) 369, 382, 812, 825, 914, 927
AcoI YGGCCR 3 cut(s) 277, 870, 972
AcsI RAATTY 4 cut(s) 139, 166, 193, 1431
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 2 cut(s) 1488, 1687
AfiI CCNNNNNNNGG 5 cut(s) 370, 616, 1168, 1503, 1504
AgsI TTSAA 3 cut(s) 165, 532, 1226
AjnI CCWGG 7 cut(s) 76, 126, 303, 594, 639, 977, 1375
Alw21I GWGCWC 4 cut(s) 85, 300, 603, 1275
Alw26I GTCTC 4 cut(s) 210, 437, 1044, 1505
AlwI GGATC 6 cut(s) 369, 382, 812, 825, 914, 927
AlwNI CAGNNNCTG 2 cut(s) 346, 1793
Ama87I CYCGRG 1 cut(s) 616
AoxI GGCC 7 cut(s) 119, 150, 277, 472, 637, 870, 972
ApoI RAATTY 4 cut(s) 139, 166, 193, 1431
Asp700I GAANNNNTTC 1 cut(s) 1083
Asp718I GGTACC 1 cut(s) 1685
AspLEI GCGC 4 cut(s) 234, 313, 315, 775
AspS9I GGNCC 5 cut(s) 17, 120, 151, 472, 637
AsuC2I CCSGG 5 cut(s) 21, 226, 617, 618, 1396
AsuHPI GGTGA 7 cut(s) 47, 96, 283, 439, 1394, 1486, 1717
AsuII TTCGAA 1 cut(s) 191
AsuNHI GCTAGC 1 cut(s) 1561
AvaI CYCGRG 1 cut(s) 616
AvaII GGWCC 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 582
BaeI ACNNNNGTAYC 2 cut(s) 1017, 1050
BalI TGGCCA 1 cut(s) 974
BamHI GGATCC 3 cut(s) 374, 817, 919
BanI GGYRCC 2 cut(s) 579, 1685
BanII GRGCYC 3 cut(s) 85, 300, 1275
BbsI GAAGAC 1 cut(s) 1715
Bbv12I GWGCWC 4 cut(s) 85, 300, 603, 1275
BccI CCATC 2 cut(s) 361, 483
BceAI ACGGC 6 cut(s) 82, 462, 572, 674, 857, 1436
BciT130I CCWGG 7 cut(s) 78, 128, 305, 596, 641, 979, 1377
BclI TGATCA 2 cut(s) 238, 1542
BcnI CCSGG 5 cut(s) 21, 226, 617, 618, 1396
BcoDI GTCTC 4 cut(s) 210, 437, 1044, 1505
BfaI CTAG 2 cut(s) 827, 1562
BfmI CTRYAG 3 cut(s) 483, 1283, 1578
BfuAI ACCTGC 1 cut(s) 259
BlpI GCTNAGC 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 616
BmgT120I GGNCC 5 cut(s) 17, 120, 151, 472, 637
BmiI GGNNCC 7 cut(s) 18, 93, 376, 581, 819, 921, 1687
BmsI GCATC 4 cut(s) 346, 349, 541, 772
BmtI GCTAGC 1 cut(s) 1565
BpiI GAAGAC 1 cut(s) 1715
Bpu1102I GCTNAGC 1 cut(s) 623
Bpu14I TTCGAA 1 cut(s) 191
BpuMI CCSGG 5 cut(s) 21, 226, 617, 618, 1396
BsaAI YACGTR 1 cut(s) 1702
BsaI GGTCTC 1 cut(s) 1044
BsaJI CCNNGG 9 cut(s) 20, 76, 126, 316, 616, 617, 1166, 1266, 1395
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 392, 422
Bsc4I CCNNNNNNNGG 5 cut(s) 370, 616, 1168, 1503, 1504
Bse1I ACTGG 2 cut(s) 280, 1261
Bse3DI GCAATG 3 cut(s) 179, 554, 962
BseBI CCWGG 7 cut(s) 78, 128, 305, 596, 641, 979, 1377
BseDI CCNNGG 9 cut(s) 20, 76, 126, 316, 616, 617, 1166, 1266, 1395
BseGI GGATG 2 cut(s) 1177, 1437
BseLI CCNNNNNNNGG 5 cut(s) 370, 616, 1168, 1503, 1504
BseMI GCAATG 3 cut(s) 179, 554, 962
BseMII CTCAG 3 cut(s) 1329, 1721, 1800
BseNI ACTGG 2 cut(s) 280, 1261
BsePI GCGCGC 1 cut(s) 311
BseRI GAGGAG 4 cut(s) 413, 506, 1540, 1768
BseSI GKGCMC 1 cut(s) 582
BsgI GTGCAG 2 cut(s) 83, 1558
Bsh1236I CGCG 3 cut(s) 313, 318, 775
BshFI GGCC 7 cut(s) 121, 152, 279, 474, 639, 872, 974
BshNI GGYRCC 2 cut(s) 579, 1685
BsiHKAI GWGCWC 4 cut(s) 85, 300, 603, 1275
BsiHKCI CYCGRG 1 cut(s) 616
BsiSI CCGG 4 cut(s) 20, 225, 617, 1395
BslFI GGGAC 4 cut(s) 30, 56, 335, 1674
BslI CCNNNNNNNGG 5 cut(s) 370, 616, 1168, 1503, 1504
BsmAI GTCTC 4 cut(s) 210, 437, 1044, 1505
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 4 cut(s) 30, 56, 335, 1674
BsmI GAATGC 2 cut(s) 760, 1042
BsnI GGCC 7 cut(s) 121, 152, 279, 474, 639, 872, 974
Bso31I GGTCTC 1 cut(s) 1044
BsoBI CYCGRG 1 cut(s) 616
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 5 cut(s) 85, 300, 582, 603, 1275
Bsp143I GATC 5 cut(s) 238, 374, 817, 919, 1542
Bsp1720I GCTNAGC 1 cut(s) 623
BspANI GGCC 7 cut(s) 121, 152, 279, 474, 639, 872, 974
BspCNI CTCAG 3 cut(s) 1328, 1722, 1799
BspFNI CGCG 3 cut(s) 313, 318, 775
BspLI GGNNCC 7 cut(s) 18, 93, 376, 581, 819, 921, 1687
BspMI ACCTGC 1 cut(s) 259
BspOI GCTAGC 1 cut(s) 1565
BspPI GGATC 6 cut(s) 369, 382, 812, 825, 914, 927
BspT104I TTCGAA 1 cut(s) 191
BspT107I GGYRCC 2 cut(s) 579, 1685
BspTNI GGTCTC 1 cut(s) 1044
BsrDI GCAATG 3 cut(s) 179, 554, 962
BsrI ACTGG 2 cut(s) 280, 1261
BssECI CCNNGG 9 cut(s) 20, 76, 126, 316, 616, 617, 1166, 1266, 1395
BssHII GCGCGC 1 cut(s) 311
BssMI GATC 5 cut(s) 238, 374, 817, 919, 1542
BssT1I CCWWGG 1 cut(s) 1266
Bst2UI CCWGG 7 cut(s) 78, 128, 305, 596, 641, 979, 1377
Bst4CI ACNGT 7 cut(s) 634, 1189, 1324, 1348, 1582, 1651, 1811
Bst6I CTCTTC 3 cut(s) 384, 1466, 1730
BstBAI YACGTR 1 cut(s) 1702
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 5 cut(s) 123, 313, 1234, 1280, 1563
BstDEI CTNAG 6 cut(s) 604, 623, 1178, 1315, 1730, 1786
BstDSI CCRYGG 2 cut(s) 316, 1166
BstF5I GGATG 2 cut(s) 1177, 1437
BstFNI CGCG 3 cut(s) 313, 318, 775
BstHHI GCGC 4 cut(s) 234, 313, 315, 775
BstKTI GATC 5 cut(s) 241, 377, 820, 922, 1545
BstMAI GTCTC 4 cut(s) 210, 437, 1044, 1505
BstMBI GATC 5 cut(s) 238, 374, 817, 919, 1542
BstMWI GCNNNNNNNGC 7 cut(s) 64, 781, 923, 1145, 1279, 1349, 1358
BstNI CCWGG 7 cut(s) 78, 128, 305, 596, 641, 979, 1377
BstNSI RCATGY 2 cut(s) 788, 971
BstSFI CTRYAG 3 cut(s) 483, 1283, 1578
BstSLI GKGCMC 1 cut(s) 582
BstUI CGCG 3 cut(s) 313, 318, 775
BstV2I GAAGAC 1 cut(s) 1715
BstX2I RGATCY 3 cut(s) 374, 817, 919
BstYI RGATCY 3 cut(s) 374, 817, 919
BsuRI GGCC 7 cut(s) 121, 152, 279, 474, 639, 872, 974
BtgI CCRYGG 2 cut(s) 316, 1166
BtsCI GGATG 2 cut(s) 1177, 1437
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 3 cut(s) 639, 895, 1329
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 5 cut(s) 123, 313, 1234, 1280, 1563
CaiI CAGNNNCTG 2 cut(s) 346, 1793
CfoI GCGC 4 cut(s) 234, 313, 315, 775
Cfr13I GGNCC 5 cut(s) 17, 120, 151, 472, 637
Cfr42I CCGCGG 1 cut(s) 319
Cfr9I CCCGGG 1 cut(s) 616
CseI GACGC 2 cut(s) 204, 1769
Csp6I GTAC 2 cut(s) 1487, 1686
CviAII CATG 8 cut(s) 26, 508, 611, 785, 934, 968, 1690, 1777
CviQI GTAC 2 cut(s) 1487, 1686
DdeI CTNAG 6 cut(s) 604, 623, 1178, 1315, 1730, 1786
DpnI GATC 5 cut(s) 240, 376, 819, 921, 1544
DpnII GATC 5 cut(s) 238, 374, 817, 919, 1542
DraI TTTAAA 1 cut(s) 1429
EaeI YGGCCR 3 cut(s) 277, 870, 972
Eam1104I CTCTTC 3 cut(s) 384, 1466, 1730
EarI CTCTTC 3 cut(s) 384, 1466, 1730
Ecl136II GAGCTC 3 cut(s) 83, 298, 1273
Eco130I CCWWGG 1 cut(s) 1266
Eco24I GRGCYC 3 cut(s) 85, 300, 1275
Eco31I GGTCTC 1 cut(s) 1044
Eco47I GGWCC 1 cut(s) 17
Eco53kI GAGCTC 3 cut(s) 83, 298, 1273
Eco57I CTGAAG 1 cut(s) 1116
Eco88I CYCGRG 1 cut(s) 616
EcoICRI GAGCTC 3 cut(s) 83, 298, 1273
EcoO109I RGGNCCY 1 cut(s) 151
EcoRI GAATTC 2 cut(s) 139, 193
EcoRII CCWGG 7 cut(s) 76, 126, 303, 594, 639, 977, 1375
EcoT14I CCWWGG 1 cut(s) 1266
EcoT22I ATGCAT 1 cut(s) 556
EcoT38I GRGCYC 3 cut(s) 85, 300, 1275
ErhI CCWWGG 1 cut(s) 1266
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 8 cut(s) 29, 511, 614, 788, 937, 971, 1693, 1780
FalI AAGNNNNNCTT 2 cut(s) 1106, 1138
FaqI GGGAC 4 cut(s) 30, 56, 335, 1674
FatI CATG 8 cut(s) 25, 507, 610, 784, 933, 967, 1689, 1776
FauI CCCGC 3 cut(s) 130, 311, 1241
FauNDI CATATG 1 cut(s) 867
FbaI TGATCA 2 cut(s) 238, 1542
FokI GGATG 2 cut(s) 1184, 1424
FriOI GRGCYC 3 cut(s) 85, 300, 1275
FspBI CTAG 2 cut(s) 827, 1562
GlaI GCGC 4 cut(s) 233, 312, 314, 774
HaeIII GGCC 7 cut(s) 121, 152, 279, 474, 639, 872, 974
HapII CCGG 4 cut(s) 20, 225, 617, 1395
HgaI GACGC 2 cut(s) 204, 1769
HhaI GCGC 4 cut(s) 234, 313, 315, 775
Hin1II CATG 8 cut(s) 29, 511, 614, 788, 937, 971, 1693, 1780
Hin6I GCGC 4 cut(s) 232, 311, 313, 773
HinP1I GCGC 4 cut(s) 232, 311, 313, 773
HinfI GANTC 8 cut(s) 6, 29, 535, 716, 1126, 1296, 1790, 1796
HpaII CCGG 4 cut(s) 20, 225, 617, 1395
HphI GGTGA 7 cut(s) 47, 96, 283, 439, 1394, 1486, 1717
Hpy166II GTNNAC 1 cut(s) 1807
Hpy188I TCNGA 8 cut(s) 91, 379, 1096, 1145, 1418, 1789, 1795, 1801
Hpy188III TCNNGA 3 cut(s) 136, 941, 1022
Hpy8I GTNNAC 1 cut(s) 1807
Hpy99I CGWCG 3 cut(s) 103, 118, 695
HpyAV CCTTC 4 cut(s) 46, 511, 1073, 1286
HpyCH4III ACNGT 7 cut(s) 634, 1189, 1324, 1348, 1582, 1651, 1811
HpyCH4IV ACGT 1 cut(s) 1701
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 781, 923, 1145, 1279, 1349, 1358
HpyF3I CTNAG 6 cut(s) 604, 623, 1178, 1315, 1730, 1786
HpySE526I ACGT 1 cut(s) 1701
Hsp92II CATG 8 cut(s) 29, 511, 614, 788, 937, 971, 1693, 1780
HspAI GCGC 4 cut(s) 232, 311, 313, 773
KpnI GGTACC 1 cut(s) 1689
Ksp22I TGATCA 2 cut(s) 238, 1542
KspI CCGCGG 1 cut(s) 319
Kzo9I GATC 5 cut(s) 238, 374, 817, 919, 1542
LmnI GCTCC 7 cut(s) 80, 91, 303, 466, 493, 1270, 1278
LweI GCATC 4 cut(s) 346, 349, 541, 772
MaeI CTAG 2 cut(s) 827, 1562
MaeII ACGT 1 cut(s) 1701
MaeIII GTNAC 7 cut(s) 102, 271, 1189, 1252, 1318, 1645, 1781
MalI GATC 5 cut(s) 240, 376, 819, 921, 1544
MboI GATC 5 cut(s) 238, 374, 817, 919, 1542
MfeI CAATTG 1 cut(s) 1668
MflI RGATCY 3 cut(s) 374, 817, 919
MhlI GDGCHC 5 cut(s) 85, 300, 582, 603, 1275
MlsI TGGCCA 1 cut(s) 974
MluNI TGGCCA 1 cut(s) 974
MlyI GAGTC 2 cut(s) 1120, 1799
MmeI TCCRAC 1 cut(s) 69
Mox20I TGGCCA 1 cut(s) 974
Mph1103I ATGCAT 1 cut(s) 556
MroXI GAANNNNTTC 1 cut(s) 1083
MscI TGGCCA 1 cut(s) 974
MseI TTAA 4 cut(s) 696, 1113, 1158, 1428
MslI CAYNNNNRTG 1 cut(s) 888
Msp20I TGGCCA 1 cut(s) 974
MspA1I CMGCKG 4 cut(s) 318, 332, 464, 1403
MspI CCGG 4 cut(s) 20, 225, 617, 1395
MunI CAATTG 1 cut(s) 1668
Mva1269I GAATGC 2 cut(s) 760, 1042
MvaI CCWGG 7 cut(s) 78, 128, 305, 596, 641, 979, 1377
MvnI CGCG 3 cut(s) 313, 318, 775
MwoI GCNNNNNNNGC 7 cut(s) 64, 781, 923, 1145, 1279, 1349, 1358
NciI CCSGG 5 cut(s) 21, 226, 617, 618, 1396
NdeI CATATG 1 cut(s) 867
NdeII GATC 5 cut(s) 238, 374, 817, 919, 1542
NheI GCTAGC 1 cut(s) 1561
NlaIII CATG 8 cut(s) 29, 511, 614, 788, 937, 971, 1693, 1780
NlaIV GGNNCC 7 cut(s) 18, 93, 376, 581, 819, 921, 1687
NmuCI GTSAC 6 cut(s) 102, 271, 1189, 1318, 1645, 1781
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 2 cut(s) 788, 971
NspV TTCGAA 1 cut(s) 191
PaqCI CACCTGC 1 cut(s) 259
PauI GCGCGC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PctI GAATGC 2 cut(s) 760, 1042
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 6 cut(s) 6, 29, 535, 716, 1296, 1796
PflMI CCANNNNNTGG 1 cut(s) 370
PfoI TCCNGGA 1 cut(s) 224
PleI GAGTC 2 cut(s) 1120, 1798
PpsI GAGTC 2 cut(s) 1120, 1798
Ppu21I YACGTR 1 cut(s) 1702
Psp124BI GAGCTC 3 cut(s) 85, 300, 1275
Psp6I CCWGG 7 cut(s) 76, 126, 303, 594, 639, 977, 1375
PspGI CCWGG 7 cut(s) 76, 126, 303, 594, 639, 977, 1375
PspN4I GGNNCC 7 cut(s) 18, 93, 376, 581, 819, 921, 1687
PspPI GGNCC 5 cut(s) 17, 120, 151, 472, 637
PstNI CAGNNNCTG 2 cut(s) 346, 1793
PsuI RGATCY 3 cut(s) 374, 817, 919
PteI GCGCGC 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 1403
RsaI GTAC 2 cut(s) 1488, 1687
RsaNI GTAC 2 cut(s) 1487, 1686
RseI CAYNNNNRTG 1 cut(s) 888
SacI GAGCTC 3 cut(s) 85, 300, 1275
SacII CCGCGG 1 cut(s) 319
SaqAI TTAA 4 cut(s) 696, 1113, 1158, 1428
Sau3AI GATC 5 cut(s) 238, 374, 817, 919, 1542
Sau96I GGNCC 5 cut(s) 17, 120, 151, 472, 637
SchI GAGTC 2 cut(s) 1120, 1799
SduI GDGCHC 5 cut(s) 85, 300, 582, 603, 1275
SfaNI GCATC 4 cut(s) 346, 349, 541, 772
SfcI CTRYAG 3 cut(s) 483, 1283, 1578
Sfr303I CCGCGG 1 cut(s) 319
SfuI TTCGAA 1 cut(s) 191
SgrBI CCGCGG 1 cut(s) 319
SinI GGWCC 1 cut(s) 17
SmaI CCCGGG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 888
SspMI CTAG 2 cut(s) 827, 1562
SstI GAGCTC 3 cut(s) 85, 300, 1275
StyI CCWWGG 1 cut(s) 1266
TaaI ACNGT 7 cut(s) 634, 1189, 1324, 1348, 1582, 1651, 1811
TaiI ACGT 1 cut(s) 1704
TaqI TCGA 3 cut(s) 116, 137, 191
TauI GCSGC 4 cut(s) 97, 282, 318, 1621
TfiI GAWTC 6 cut(s) 6, 29, 535, 716, 1296, 1796
Tru1I TTAA 4 cut(s) 696, 1113, 1158, 1428
Tru9I TTAA 4 cut(s) 696, 1113, 1158, 1428
TscAI CASTG 3 cut(s) 639, 895, 1329
TseFI GTSAC 6 cut(s) 102, 271, 1189, 1318, 1645, 1781
Tsp45I GTSAC 6 cut(s) 102, 271, 1189, 1318, 1645, 1781
TspDTI ATGAA 5 cut(s) 402, 445, 1167, 1426, 1587
TspGWI ACGGA 2 cut(s) 527, 1535
TspMI CCCGGG 1 cut(s) 616
TspRI CASTG 3 cut(s) 639, 895, 1329
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 4 cut(s) 139, 166, 193, 1431
XceI RCATGY 2 cut(s) 788, 971
XcmI CCANNNNNNNNNTGG 2 cut(s) 205, 1253
XmaI CCCGGG 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 2 cut(s) 827, 1562
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.