MD04G1120300.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009698
Physical Location & Seq
Forward (+)
20654918 .. 20657972
3055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1120300.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGGCAAAGGGTACTGGGTGTTTAAACAAAGGTGAGGCGCTGTATGATAATTCTTCTAGAATTGAGCCTAAGCGTTCTCATCAGTGGTTCATGGATGGTCCTGAAGTAGGGCTGTTCCCCAACAAGAAACAGGCGGTTGAAGTTCCGCACAACAATCTGTATTCGGGAATGTTAACTGCAAATGTTTCTCCGTGGGGAAATGTTCCTAGTTTTCAATCATTCTCTGGCCATTTCACTGAAAGGCTATTTGATTCTGAAACAGACCGTGCTGTGAATTTTGATGAAAGGAGCATTCCGTCTGTTGGAACGGAAAAGATGAATTTGGCTAGAAAAGTTAGTGAGGATTTATTTGGGAATGACTCCTCATTTGGTCTATCTATGTCCAATACACTAGAAGATCCTAGATCCAGTCCTAACTACGGCGGATTTAGAAAAGTCAAAGTTAGCGAGGTAAGGGACTCTGAGAATGTCATGCCTGTTTCAATCAGACAAGCTTATGATCAGGCAGATAATGATGCTGTGTTGGCGGCTCACGCTTACAAGGCTGACGACAACTCAGCATCCACGGGCCTCATTTATAAGAAGGGGGATGACAACTTCATATCCATGAGTGATACCTACAGCAGGGCAGATAACGGCTTCATTTCAATTGGGCAACCCTTTAACAAGGGGGATGAAAGCATGTCGATTGGTCAATCATACAAGGAGAGCAATAACACCCTATCAGTGCGTCAGACATTCAACAAAAGTGACAATAATATCATTTCTATCGGCCAAACCTACAACAACGCAGAGGAAAGTACCATGTCAACGGGTCATTTCTACAATAAAGGGGAAGAGAGTACTATATCCGTTGGTCATGCCTACAGCAAGGGTGACAACAATATGTTATCGATTGGTCACTCTTATGATAAGCGGGAGAGTACTATCATATCCTTTGGCAGATGTGATGATGATGATGCAAATACCTCTGCAATCTCTGGTTATGAGCTTCTGATGGGTCAACCTTTTTTTAAAACAGAAGCTGTGAATGAGAGAGAGTTGGGCAAATCAAAGTCCTGTGTACTTGTTAATATACCTCATATAACTGCTGGAAATGAAAATGTTTTGAAAAAGAAAGTTGAACATAAAACGTCTAAGAAGGTTCCCCCGAACAACTTCCCATCAAATGTTCGAAGTTTACTGTCAACAGGTATGCTGGATGGAGTTCCTGTAAAATATACGGCCTGGTCACGGGAGAAGGAGCTGCAGGGTGTGATCAAAGGTTCTGGGTATCTATGTGGCTGTCAGTCGTGCAACTTCTCAAAGGTAATCAATGCATATGAATTTGAGCGTCACGCTGATTGCAAAACAAAACACCCGAATAATCACATATATTTTGAGAATGGCAAAACTATATATGGGATTGTTCAAGAGCTCAGGAGCACACCGCAGAACATGCTGTTTGAAGTTATTCAGACTATTACTGGTTCGCCTATCAATCAGAAATCCTTCCACCTTTGGAAAGAATCCTTTTTGGCAGCAACACGTGAACTTCAGCGTATATATGGCAAGGAAGAGGGAAAGCAACTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

526

Amino Acids

58.4

Weight (kDa)

6.26

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 418 - 472 2.8e-14 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AciI CCGC 6 cut(s) 134, 146, 423, 527, 916, 1430
AclWI GGATC 2 cut(s) 392, 399
AcoI YGGCCR 2 cut(s) 226, 772
AcsI RAATTY 3 cut(s) 274, 319, 1325
AcuI CTGAAG 2 cut(s) 123, 1520
AcvI CACGTG 1 cut(s) 1529
AfaI GTAC 5 cut(s) 13, 802, 844, 925, 1065
AfiI CCNNNNNNNGG 5 cut(s) 107, 302, 419, 624, 1233
AflIII ACRYGT 1 cut(s) 1526
AgsI TTSAA 9 cut(s) 140, 215, 483, 648, 742, 1111, 1124, 1412, 1448
AjnI CCWGG 1 cut(s) 1226
AluBI AGCT 5 cut(s) 494, 991, 1025, 1246, 1417
AluI AGCT 5 cut(s) 494, 991, 1025, 1246, 1417
Alw21I GWGCWC 2 cut(s) 1419, 1427
AlwI GGATC 2 cut(s) 392, 399
AlwNI CAGNNNCTG 1 cut(s) 1025
AoxI GGCC 4 cut(s) 226, 568, 772, 1224
ApeKI GCWGC 2 cut(s) 1246, 1520
ApoI RAATTY 3 cut(s) 274, 319, 1325
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
Asp700I GAANNNNTTC 3 cut(s) 1157, 1452, 1490
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 98, 568
AsuHPI GGTGA 2 cut(s) 44, 887
AsuII TTCGAA 1 cut(s) 1174
AvaII GGWCC 1 cut(s) 98
BalI TGGCCA 1 cut(s) 228
BanII GRGCYC 1 cut(s) 1419
BbrPI CACGTG 1 cut(s) 1529
Bbv12I GWGCWC 2 cut(s) 1419, 1427
BbvI GCAGC 2 cut(s) 1233, 1532
BccI CCATC 4 cut(s) 89, 991, 1171, 1196
BceAI ACGGC 3 cut(s) 436, 652, 1239
BciT130I CCWGG 1 cut(s) 1228
BclI TGATCA 2 cut(s) 499, 1257
BfaI CTAG 5 cut(s) 57, 207, 327, 392, 402
BfmI CTRYAG 3 cut(s) 619, 865, 1247
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 3 cut(s) 528, 1247, 1521
BlsI GCNGC 3 cut(s) 529, 1248, 1522
BmcAI AGTACT 2 cut(s) 844, 925
Bme1390I CCNGG 1 cut(s) 1228
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 2 cut(s) 98, 568
BmiI GGNNCC 1 cut(s) 1146
BmrFI CCNGG 1 cut(s) 1228
BmrI ACTGGG 1 cut(s) 24
BmsI GCATC 3 cut(s) 505, 569, 949
BmuI ACTGGG 1 cut(s) 24
Bpu10I CCTNAGC 2 cut(s) 69, 1418
Bpu14I TTCGAA 1 cut(s) 1174
Bsa29I ATCGAT 1 cut(s) 893
BsaAI YACGTR 1 cut(s) 1529
BsaJI CCNNGG 2 cut(s) 191, 564
BsaXI ACNNNNNCTCC 2 cut(s) 1197, 1227
Bsc4I CCNNNNNNNGG 5 cut(s) 107, 302, 419, 624, 1233
Bse1I ACTGG 3 cut(s) 19, 408, 1471
BseBI CCWGG 1 cut(s) 1228
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 2 cut(s) 191, 564
BseGI GGATG 5 cut(s) 100, 560, 595, 679, 1207
BseLI CCNNNNNNNGG 5 cut(s) 107, 302, 419, 624, 1233
BseMII CTCAG 3 cut(s) 453, 570, 1432
BseNI ACTGG 3 cut(s) 19, 408, 1471
BseRI GAGGAG 1 cut(s) 352
BseXI GCAGC 2 cut(s) 1233, 1532
BshFI GGCC 4 cut(s) 228, 570, 774, 1226
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 1419, 1427
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 5 cut(s) 107, 302, 419, 624, 1233
BsmFI GGGAC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 4 cut(s) 228, 570, 774, 1226
Bsp119I TTCGAA 1 cut(s) 1174
Bsp1286I GDGCHC 2 cut(s) 1419, 1427
Bsp143I GATC 4 cut(s) 397, 404, 499, 1257
BspACI CCGC 6 cut(s) 134, 146, 423, 527, 916, 1430
BspANI GGCC 4 cut(s) 228, 570, 774, 1226
BspCNI CTCAG 3 cut(s) 454, 569, 1431
BspDI ATCGAT 1 cut(s) 893
BspHI TCATGA 1 cut(s) 1574
BspLI GGNNCC 1 cut(s) 1146
BspMAI CTGCAG 1 cut(s) 1251
BspPI GGATC 2 cut(s) 392, 399
BspT104I TTCGAA 1 cut(s) 1174
BsrI ACTGG 3 cut(s) 19, 408, 1471
BssECI CCNNGG 2 cut(s) 191, 564
BssMI GATC 4 cut(s) 397, 404, 499, 1257
Bst2UI CCWGG 1 cut(s) 1228
Bst4CI ACNGT 2 cut(s) 266, 1185
Bst6I CTCTTC 2 cut(s) 831, 1551
BstAPI GCANNNNNTGC 1 cut(s) 1438
BstBAI YACGTR 1 cut(s) 1529
BstBI TTCGAA 1 cut(s) 1174
BstDEI CTNAG 5 cut(s) 69, 462, 556, 1137, 1418
BstDSI CCRYGG 2 cut(s) 191, 564
BstENI CCTNNNNNAGG 2 cut(s) 105, 622
BstF5I GGATG 5 cut(s) 100, 560, 595, 679, 1207
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 400, 407, 502, 1260
BstMBI GATC 4 cut(s) 397, 404, 499, 1257
BstMWI GCNNNNNNNGC 4 cut(s) 524, 533, 542, 1438
BstNI CCWGG 1 cut(s) 1228
BstNSI RCATGY 2 cut(s) 685, 1441
BstSCI CCNGG 1 cut(s) 1226
BstSFI CTRYAG 3 cut(s) 619, 865, 1247
BstV1I GCAGC 2 cut(s) 1233, 1532
BstX2I RGATCY 2 cut(s) 397, 404
BstYI RGATCY 2 cut(s) 397, 404
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 4 cut(s) 228, 570, 774, 1226
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 2 cut(s) 191, 564
BtsCI GGATG 5 cut(s) 100, 560, 595, 679, 1207
BtsIMutI CAGTG 3 cut(s) 89, 234, 732
CaiI CAGNNNCTG 1 cut(s) 1025
CciI TCATGA 1 cut(s) 1574
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 98, 568
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 2 cut(s) 719, 1322
Csp6I GTAC 5 cut(s) 12, 801, 843, 924, 1064
CviAII CATG 8 cut(s) 91, 472, 607, 682, 805, 860, 1438, 1575
CviQI GTAC 5 cut(s) 12, 801, 843, 924, 1064
DdeI CTNAG 5 cut(s) 69, 462, 556, 1137, 1418
DpnI GATC 4 cut(s) 399, 406, 501, 1259
DpnII GATC 4 cut(s) 397, 404, 499, 1257
DraI TTTAAA 2 cut(s) 24, 1015
EaeI YGGCCR 2 cut(s) 226, 772
Eam1104I CTCTTC 2 cut(s) 831, 1551
EarI CTCTTC 2 cut(s) 831, 1551
EciI GGCGGA 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 1417
Eco24I GRGCYC 1 cut(s) 1419
Eco47I GGWCC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 1417
Eco57I CTGAAG 2 cut(s) 123, 1520
Eco72I CACGTG 1 cut(s) 1529
EcoICRI GAGCTC 1 cut(s) 1417
EcoNI CCTNNNNNAGG 2 cut(s) 105, 622
EcoRII CCWGG 1 cut(s) 1226
EcoT22I ATGCAT 1 cut(s) 1321
EcoT38I GRGCYC 1 cut(s) 1419
FaeI CATG 8 cut(s) 94, 475, 610, 685, 808, 863, 1441, 1578
FaqI GGGAC 1 cut(s) 470
FatI CATG 8 cut(s) 90, 471, 606, 681, 804, 859, 1437, 1574
FauI CCCGC 1 cut(s) 909
FauNDI CATATG 1 cut(s) 1321
FbaI TGATCA 2 cut(s) 499, 1257
Fnu4HI GCNGC 3 cut(s) 528, 1247, 1521
FokI GGATG 5 cut(s) 107, 547, 602, 686, 1214
FriOI GRGCYC 1 cut(s) 1419
Fsp4HI GCNGC 3 cut(s) 528, 1247, 1521
FspBI CTAG 5 cut(s) 57, 207, 327, 392, 402
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 528, 1247, 1521
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 4 cut(s) 228, 570, 774, 1226
HgaI GACGC 2 cut(s) 719, 1322
HhaI GCGC 1 cut(s) 40
Hin1II CATG 8 cut(s) 94, 475, 610, 685, 808, 863, 1441, 1578
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 4 cut(s) 174, 810, 1004, 1188
HindII GTYRAC 4 cut(s) 174, 810, 1004, 1188
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 4 cut(s) 251, 359, 458, 1508
HpaI GTTAAC 1 cut(s) 174
HphI GGTGA 2 cut(s) 44, 887
Hpy166II GTNNAC 7 cut(s) 174, 810, 1004, 1064, 1181, 1188, 1532
Hpy188I TCNGA 7 cut(s) 256, 463, 488, 735, 996, 1458, 1485
Hpy188III TCNNGA 6 cut(s) 57, 101, 165, 1412, 1420, 1575
Hpy8I GTNNAC 7 cut(s) 174, 810, 1004, 1064, 1181, 1188, 1532
HpyAV CCTTC 4 cut(s) 577, 1135, 1234, 1501
HpyCH4III ACNGT 2 cut(s) 266, 1185
HpyCH4IV ACGT 2 cut(s) 1133, 1528
HpyCH4V TGCA 7 cut(s) 179, 962, 974, 1249, 1296, 1319, 1347
HpyF10VI GCNNNNNNNGC 4 cut(s) 524, 533, 542, 1438
HpyF3I CTNAG 5 cut(s) 69, 462, 556, 1137, 1418
HpySE526I ACGT 2 cut(s) 1133, 1528
Hsp92II CATG 8 cut(s) 94, 475, 610, 685, 808, 863, 1441, 1578
HspAI GCGC 1 cut(s) 38
Ksp22I TGATCA 2 cut(s) 499, 1257
KspAI GTTAAC 1 cut(s) 174
Kzo9I GATC 4 cut(s) 397, 404, 499, 1257
LmnI GCTCC 3 cut(s) 288, 1243, 1422
Lsp1109I GCAGC 2 cut(s) 1233, 1532
LweI GCATC 3 cut(s) 505, 569, 949
MaeI CTAG 5 cut(s) 57, 207, 327, 392, 402
MaeII ACGT 2 cut(s) 1133, 1528
MaeIII GTNAC 5 cut(s) 749, 875, 899, 1230, 1334
MalI GATC 4 cut(s) 399, 406, 501, 1259
MboI GATC 4 cut(s) 397, 404, 499, 1257
MboII GAAGA 4 cut(s) 45, 407, 848, 1568
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 2 cut(s) 397, 404
MhlI GDGCHC 2 cut(s) 1419, 1427
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 6 cut(s) 49, 60, 274, 319, 648, 1325
MluNI TGGCCA 1 cut(s) 228
MlyI GAGTC 2 cut(s) 353, 452
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 9 cut(s) 28, 334, 373, 442, 581, 787, 979, 1089, 1552
Mox20I TGGCCA 1 cut(s) 228
Mph1103I ATGCAT 1 cut(s) 1321
MroXI GAANNNNTTC 3 cut(s) 1157, 1452, 1490
MscI TGGCCA 1 cut(s) 228
MseI TTAA 5 cut(s) 23, 173, 663, 1014, 1071
MslI CAYNNNNRTG 2 cut(s) 605, 906
Msp20I TGGCCA 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 1228
MssI GTTTAAAC 1 cut(s) 24
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 291
MvaI CCWGG 1 cut(s) 1228
MwoI GCNNNNNNNGC 4 cut(s) 524, 533, 542, 1438
NdeI CATATG 1 cut(s) 1321
NdeII GATC 4 cut(s) 397, 404, 499, 1257
NlaIII CATG 8 cut(s) 94, 475, 610, 685, 808, 863, 1441, 1578
NlaIV GGNNCC 1 cut(s) 1146
NmuCI GTSAC 5 cut(s) 749, 875, 899, 1230, 1334
NsiI ATGCAT 1 cut(s) 1321
NspI RCATGY 2 cut(s) 685, 1441
NspV TTCGAA 1 cut(s) 1174
PagI TCATGA 1 cut(s) 1574
PctI GAATGC 1 cut(s) 291
PdmI GAANNNNTTC 3 cut(s) 1157, 1452, 1490
PfeI GAWTC 2 cut(s) 251, 1508
PkrI GCNGC 3 cut(s) 529, 1248, 1522
PleI GAGTC 2 cut(s) 353, 452
PmaCI CACGTG 1 cut(s) 1529
PmeI GTTTAAAC 1 cut(s) 24
PmlI CACGTG 1 cut(s) 1529
PpsI GAGTC 2 cut(s) 353, 452
Ppu21I YACGTR 1 cut(s) 1529
PsiI TTATAA 1 cut(s) 579
Psp124BI GAGCTC 1 cut(s) 1419
Psp6I CCWGG 1 cut(s) 1226
PspCI CACGTG 1 cut(s) 1529
PspGI CCWGG 1 cut(s) 1226
PspN4I GGNNCC 1 cut(s) 1146
PspPI GGNCC 2 cut(s) 98, 568
PstI CTGCAG 1 cut(s) 1251
PstNI CAGNNNCTG 1 cut(s) 1025
PsuI RGATCY 2 cut(s) 397, 404
RsaI GTAC 5 cut(s) 13, 802, 844, 925, 1065
RsaNI GTAC 5 cut(s) 12, 801, 843, 924, 1064
RseI CAYNNNNRTG 2 cut(s) 605, 906
SacI GAGCTC 1 cut(s) 1419
SaqAI TTAA 5 cut(s) 23, 173, 663, 1014, 1071
SatI GCNGC 3 cut(s) 528, 1247, 1521
Sau3AI GATC 4 cut(s) 397, 404, 499, 1257
Sau96I GGNCC 2 cut(s) 98, 568
ScaI AGTACT 2 cut(s) 844, 925
SchI GAGTC 2 cut(s) 353, 452
ScrFI CCNGG 1 cut(s) 1228
SduI GDGCHC 2 cut(s) 1419, 1427
SfaNI GCATC 3 cut(s) 505, 569, 949
SfcI CTRYAG 3 cut(s) 619, 865, 1247
SfuI TTCGAA 1 cut(s) 1174
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 605, 906
Sse9I AATT 6 cut(s) 49, 60, 274, 319, 648, 1325
SsiI CCGC 6 cut(s) 134, 146, 423, 527, 916, 1430
SspMI CTAG 5 cut(s) 57, 207, 327, 392, 402
SstI GAGCTC 1 cut(s) 1419
StyD4I CCNGG 1 cut(s) 1226
TaaI ACNGT 2 cut(s) 266, 1185
TaiI ACGT 2 cut(s) 1136, 1531
TaqI TCGA 3 cut(s) 686, 893, 1174
TasI AATT 6 cut(s) 49, 60, 274, 319, 648, 1325
TatI WGTACW 3 cut(s) 842, 923, 1063
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 2 cut(s) 251, 1508
Tru1I TTAA 5 cut(s) 23, 173, 663, 1014, 1071
Tru9I TTAA 5 cut(s) 23, 173, 663, 1014, 1071
TscAI CASTG 3 cut(s) 89, 241, 732
TseFI GTSAC 5 cut(s) 749, 875, 899, 1230, 1334
TseI GCWGC 2 cut(s) 1246, 1520
Tsp45I GTSAC 5 cut(s) 749, 875, 899, 1230, 1334
TspDTI ATGAA 9 cut(s) 79, 297, 332, 589, 631, 690, 1113, 1338, 1563
TspGWI ACGGA 4 cut(s) 180, 285, 323, 841
TspRI CASTG 3 cut(s) 89, 241, 732
VpaK11BI GGWCC 1 cut(s) 98
XagI CCTNNNNNAGG 2 cut(s) 105, 622
XapI RAATTY 3 cut(s) 274, 319, 1325
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 2 cut(s) 685, 1441
XmnI GAANNNNTTC 3 cut(s) 1157, 1452, 1490
XspI CTAG 5 cut(s) 57, 207, 327, 392, 402
ZrmI AGTACT 2 cut(s) 844, 925
Zsp2I ATGCAT 1 cut(s) 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.