Rroxscaffold_6G00413670

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009698
Physical Location & Seq
Forward (+)
36042063 .. 36045246
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00413670.1

Sequence Viewer

Length: 1590 bp
ATGTCTTTTCAGAATCAAGGCTTTTGGATGGCAAAGGGTACCGGGGGTGTAAATGATGGTGAGAGGCTTTATGATAATTCTTCTGCAACTGAGCCTAAGCGTTCTCATCAATGGTTCATGGATGGTCCTGAGGTAGAGTTGTTCCCAAACAAGAAACAGGCTATTGAAGGTCCAGGCACCAATCTATTTTCAGGCTTGTTGAACGCAAATGTTTCTCCATGGGGAAATGTTCCCAGTTTTCAGTCATTCTCTGGCCAGTTCACTGAAAGGTTAGTTGATTCTGAAGCAGACAGAGCCGTGAATTTTGAGGACAGAAACGTTTCATCAGTTGGCTCAGAAAAAATGAGAAAAGTTAATGAGGATCTATTTGGGAATGATTCCTCTTTTGGTTTATCTATGTCCCATTCACTGGAAGATCCTAGGTTGAGTCCTAACTATGGTGGTTTTAGAAAAGTGAAAGTCAGTGAGGTAAAGGACTCTGAGAATGTCATGCGTGTTTCAGTTGGACATGCTTATAATCCGGGAGATAATGATGCCATGTTGGCGCCTCATGTTTACAAGGTGGATGACAACTCAGGATCAATGGGTCTCACTTATAAGAAAGAGGATGAGAACTTCATAACCATGAGTGATTCCTATAATAGGGCAGATAACAATTTTAGATCAATGGGACAACCCTTTACTAAAGGGGATGAGTACATAGGAATTGGTCAAACATACAAGGAGAATAGTAATACCCTATTGAGGAGTCGAACATTCAACAAGGGTGACAGTACTATCATCTCGGTTGGTGAAACCTACAACAAGGCAGATGAAAGTACCATGTCAACTGGTCATATTTATAATAAAGGAGAAGAGATATCAATGGGTCATGACTATAGCAAGGTCGACAACAATATGTTGTCAATTGGTCGCTCTTACAATAAGGGAGAGAGTACTATCATATCCTTTGGTGGGTGTGATGATGATGATGTAAATACCTCAACAATCTCTGGCTATGAACTTCTTATGGGTCAATCTTTTCCAAAAACAGAAGCTATGAATGAGAAAGACTTGGGTAAATTAAATGCTGATGCATTTGTAAATCAACCTCATATAATTGCTGGAGCTGAAACTGTTTCCAGAAAGAAGGATGACCAGAAAATGTCTAAGAAGTCTCCTTCAAACAACTTCCCTTCGAATGTGCGAAGTTTGCTATCAACTGGTATGCTGGATGGAGTTCCTGTAAAGTATATTGCCTGGTCACGGGAGAAGGAGCTGCAGGCTGTAATTAAGGGTTCTGGGTATCTATGTGGCTGTCAGTCATGTAACTTCTCTAAGGTAATCAATGCATATGAATTTGAGCGTCATGCTAGTTGCAAAACAAAACACCCAAATAACCACATATATTTTGAGAATGGGAAGACCGTCTATGGGATTGTGCAAGAGCTCAGGAGCACACCTCAGAATATGTTGTTTGACGTTATTCAGACTATTACTGGTTCACAAATCAATCAGAAATCCTTCCGTCTTTGGAAAGAATCATTTTTGGCAGCAACACGTGAACTTCAACGTATATATGGCAAGGATGAAGTGAAACAATTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

529

Amino Acids

58.97

Weight (kDa)

5.8

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 423 - 476 4.6e-13 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 516, 597, 843
Acc65I GGTACC 1 cut(s) 38
AccB1I GGYRCC 3 cut(s) 38, 176, 544
AccB7I CCANNNNNTGG 1 cut(s) 409
AccI GTMKAC 1 cut(s) 888
AclI AACGTT 1 cut(s) 318
AclWI GGATC 3 cut(s) 369, 410, 586
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 301, 1337
AcuI CTGAAG 1 cut(s) 303
AcvI CACGTG 1 cut(s) 1541
AcyI GRCGYC 1 cut(s) 545
AfaI GTAC 5 cut(s) 40, 698, 775, 820, 937
AfiI CCNNNNNNNGG 7 cut(s) 409, 437, 642, 744, 954, 1245, 1413
AflIII ACRYGT 1 cut(s) 1538
AgsI TTSAA 5 cut(s) 167, 202, 760, 1164, 1550
AjnI CCWGG 2 cut(s) 172, 1238
AluBI AGCT 4 cut(s) 1037, 1109, 1258, 1429
AluI AGCT 4 cut(s) 1037, 1109, 1258, 1429
Alw21I GWGCWC 2 cut(s) 1431, 1439
Alw26I GTCTC 2 cut(s) 593, 1161
AlwI GGATC 3 cut(s) 369, 410, 586
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 1258, 1532
ApoI RAATTY 2 cut(s) 301, 1337
Asp700I GAANNNNTTC 2 cut(s) 319, 1502
Asp718I GGTACC 1 cut(s) 38
AspA2I CCTAGG 1 cut(s) 419
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 125, 170
AsuC2I CCSGG 2 cut(s) 43, 522
AsuHPI GGTGA 3 cut(s) 71, 779, 803
AsuII TTCGAA 1 cut(s) 1178
AvaII GGWCC 2 cut(s) 125, 170
AvrII CCTAGG 1 cut(s) 419
AxyI CCTNAGG 1 cut(s) 129
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 3 cut(s) 38, 176, 544
BanII GRGCYC 1 cut(s) 1431
BbrPI CACGTG 1 cut(s) 1541
BbsI GAAGAC 1 cut(s) 1409
Bbv12I GWGCWC 2 cut(s) 1431, 1439
BbvI GCAGC 2 cut(s) 1245, 1544
BccI CCATC 4 cut(s) 22, 50, 116, 1208
BceAI ACGGC 1 cut(s) 281
BciT130I CCWGG 2 cut(s) 174, 1240
BcnI CCSGG 2 cut(s) 43, 522
BcoDI GTCTC 2 cut(s) 593, 1161
BfaI CTAG 2 cut(s) 420, 1353
BfmI CTRYAG 2 cut(s) 877, 1259
BfoI RGCGCY 1 cut(s) 548
BglI GCCNNNNNGGC 1 cut(s) 542
BisI GCNGC 2 cut(s) 1259, 1533
BlnI CCTAGG 1 cut(s) 419
BlsI GCNGC 2 cut(s) 1260, 1534
BmcAI AGTACT 2 cut(s) 775, 937
Bme1390I CCNGG 4 cut(s) 43, 174, 522, 1240
Bme18I GGWCC 2 cut(s) 125, 170
BmgT120I GGNCC 2 cut(s) 125, 170
BmiI GGNNCC 3 cut(s) 40, 178, 546
BmrFI CCNGG 4 cut(s) 43, 174, 522, 1240
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 2 cut(s) 523, 1063
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 1409
BplI GAGNNNNNCTC 2 cut(s) 1426, 1458
BpmI CTGGAG 1 cut(s) 1125
Bpu10I CCTNAGC 2 cut(s) 96, 1430
Bpu14I TTCGAA 1 cut(s) 1178
BpuMI CCSGG 2 cut(s) 43, 522
BsaAI YACGTR 1 cut(s) 1541
BsaHI GRCGYC 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 42, 218, 419
BsaXI ACNNNNNCTCC 2 cut(s) 1209, 1239
Bsc4I CCNNNNNNNGG 7 cut(s) 409, 437, 642, 744, 954, 1245, 1413
Bse1I ACTGG 6 cut(s) 234, 256, 414, 835, 1207, 1483
Bse21I CCTNAGG 1 cut(s) 129
BseBI CCWGG 2 cut(s) 174, 1240
BseDI CCNNGG 3 cut(s) 42, 218, 419
BseGI GGATG 8 cut(s) 33, 127, 571, 613, 697, 1138, 1219, 1573
BseLI CCNNNNNNNGG 7 cut(s) 409, 437, 642, 744, 954, 1245, 1413
BseMII CTCAG 7 cut(s) 81, 120, 348, 471, 588, 1444, 1457
BseNI ACTGG 6 cut(s) 234, 256, 414, 835, 1207, 1483
BseRI GAGGAG 1 cut(s) 760
BseXI GCAGC 2 cut(s) 1245, 1544
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 3 cut(s) 38, 176, 544
BsiHKAI GWGCWC 2 cut(s) 1431, 1439
BsiSI CCGG 2 cut(s) 42, 521
BslFI GGGAC 2 cut(s) 385, 684
BslI CCNNNNNNNGG 7 cut(s) 409, 437, 642, 744, 954, 1245, 1413
BsmAI GTCTC 2 cut(s) 593, 1161
BsmFI GGGAC 2 cut(s) 385, 684
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 1178
Bsp1286I GDGCHC 2 cut(s) 1431, 1439
Bsp143I GATC 4 cut(s) 361, 415, 578, 662
Bsp19I CCATGG 1 cut(s) 218
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 7 cut(s) 82, 121, 347, 472, 587, 1443, 1456
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 3 cut(s) 40, 178, 546
BspMAI CTGCAG 1 cut(s) 1263
BspPI GGATC 3 cut(s) 369, 410, 586
BspT104I TTCGAA 1 cut(s) 1178
BspT107I GGYRCC 3 cut(s) 38, 176, 544
BspTNI GGTCTC 1 cut(s) 593
BsrI ACTGG 6 cut(s) 234, 256, 414, 835, 1207, 1483
BssECI CCNNGG 3 cut(s) 42, 218, 419
BssMI GATC 4 cut(s) 361, 415, 578, 662
BssNI GRCGYC 1 cut(s) 545
BssT1I CCWWGG 2 cut(s) 218, 419
Bst2UI CCWGG 2 cut(s) 174, 1240
Bst4CI ACNGT 3 cut(s) 773, 1117, 1408
Bst6I CTCTTC 1 cut(s) 849
BstACI GRCGYC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 1541
BstBI TTCGAA 1 cut(s) 1178
BstC8I GCNNGC 1 cut(s) 1263
BstDSI CCRYGG 1 cut(s) 218
BstENI CCTNNNNNAGG 1 cut(s) 640
BstF5I GGATG 8 cut(s) 33, 127, 571, 613, 697, 1138, 1219, 1573
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 4 cut(s) 364, 418, 581, 665
BstMAI GTCTC 2 cut(s) 593, 1161
BstMBI GATC 4 cut(s) 361, 415, 578, 662
BstMWI GCNNNNNNNGC 3 cut(s) 293, 542, 1192
BstNI CCWGG 2 cut(s) 174, 1240
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 4 cut(s) 41, 172, 520, 1238
BstSFI CTRYAG 2 cut(s) 877, 1259
BstV1I GCAGC 2 cut(s) 1245, 1544
BstV2I GAAGAC 1 cut(s) 1409
BstX2I RGATCY 2 cut(s) 361, 415
BstYI RGATCY 2 cut(s) 361, 415
Bsu36I CCTNAGG 1 cut(s) 129
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 218
BtsCI GGATG 8 cut(s) 33, 127, 571, 613, 697, 1138, 1219, 1573
BtsIMutI CAGTG 3 cut(s) 261, 407, 469
Cac8I GCNNGC 1 cut(s) 1263
CciI TCATGA 1 cut(s) 871
CfoI GCGC 1 cut(s) 547
Cfr13I GGNCC 2 cut(s) 125, 170
CseI GACGC 1 cut(s) 1334
Csp6I GTAC 5 cut(s) 39, 697, 774, 819, 936
CviQI GTAC 5 cut(s) 39, 697, 774, 819, 936
DinI GGCGCC 1 cut(s) 546
DpnI GATC 4 cut(s) 363, 417, 580, 664
DpnII GATC 4 cut(s) 361, 415, 578, 662
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
Ecl136II GAGCTC 1 cut(s) 1429
Eco130I CCWWGG 2 cut(s) 218, 419
Eco24I GRGCYC 1 cut(s) 1431
Eco31I GGTCTC 1 cut(s) 593
Eco32I GATATC 1 cut(s) 861
Eco47I GGWCC 2 cut(s) 125, 170
Eco53kI GAGCTC 1 cut(s) 1429
Eco57I CTGAAG 1 cut(s) 303
Eco72I CACGTG 1 cut(s) 1541
Eco81I CCTNAGG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 1429
EcoNI CCTNNNNNAGG 1 cut(s) 640
EcoRII CCWGG 2 cut(s) 172, 1238
EcoRV GATATC 1 cut(s) 861
EcoT14I CCWWGG 2 cut(s) 218, 419
EcoT22I ATGCAT 2 cut(s) 1078, 1333
EcoT38I GRGCYC 1 cut(s) 1431
EgeI GGCGCC 1 cut(s) 546
EheI GGCGCC 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 218, 419
FaqI GGGAC 2 cut(s) 385, 684
FauNDI CATATG 1 cut(s) 1333
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 2 cut(s) 1259, 1533
FokI GGATG 8 cut(s) 40, 134, 578, 620, 704, 1145, 1226, 1580
FriOI GRGCYC 1 cut(s) 1431
Fsp4HI GCNGC 2 cut(s) 1259, 1533
FspBI CTAG 2 cut(s) 420, 1353
GlaI GCGC 1 cut(s) 546
GluI GCNGC 2 cut(s) 1259, 1533
GsuI CTGGAG 1 cut(s) 1125
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 1 cut(s) 255
HapII CCGG 2 cut(s) 42, 521
HgaI GACGC 1 cut(s) 1334
HhaI GCGC 1 cut(s) 547
Hin1I GRCGYC 1 cut(s) 545
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 2 cut(s) 828, 889
HindII GTYRAC 2 cut(s) 828, 889
HinfI GANTC 8 cut(s) 13, 278, 377, 427, 476, 632, 748, 1520
HpaII CCGG 2 cut(s) 42, 521
HphI GGTGA 3 cut(s) 71, 779, 803
Hpy166II GTNNAC 6 cut(s) 261, 556, 828, 889, 1484, 1544
Hpy188I TCNGA 7 cut(s) 12, 283, 337, 481, 1446, 1470, 1497
Hpy188III TCNNGA 5 cut(s) 128, 576, 872, 1122, 1432
Hpy8I GTNNAC 6 cut(s) 261, 556, 828, 889, 1484, 1544
HpyAV CCTTC 6 cut(s) 161, 1123, 1170, 1185, 1246, 1513
HpyCH4III ACNGT 3 cut(s) 773, 1117, 1408
HpyCH4IV ACGT 4 cut(s) 318, 1461, 1540, 1552
HpyCH4V TGCA 6 cut(s) 86, 1076, 1261, 1331, 1359, 1423
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 542, 1192
HpySE526I ACGT 4 cut(s) 318, 1461, 1540, 1552
Hsp92I GRCGYC 1 cut(s) 545
HspAI GCGC 1 cut(s) 545
KasI GGCGCC 1 cut(s) 544
KpnI GGTACC 1 cut(s) 42
Kzo9I GATC 4 cut(s) 361, 415, 578, 662
LmnI GCTCC 3 cut(s) 1106, 1255, 1434
Lsp1109I GCAGC 2 cut(s) 1245, 1544
LweI GCATC 2 cut(s) 523, 1063
MaeI CTAG 2 cut(s) 420, 1353
MaeII ACGT 4 cut(s) 318, 1461, 1540, 1552
MaeIII GTNAC 3 cut(s) 767, 1242, 1307
MalI GATC 4 cut(s) 363, 417, 580, 664
MboI GATC 4 cut(s) 361, 415, 578, 662
MboII GAAGA 4 cut(s) 72, 425, 866, 1414
MfeI CAATTG 2 cut(s) 906, 1580
MflI RGATCY 2 cut(s) 361, 415
MhlI GDGCHC 2 cut(s) 1431, 1439
MlsI TGGCCA 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 255
Mly113I GGCGCC 1 cut(s) 545
MlyI GAGTC 3 cut(s) 436, 470, 757
MmeI TCCRAC 1 cut(s) 484
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 2 cut(s) 1078, 1333
MroXI GAANNNNTTC 2 cut(s) 319, 1502
MscI TGGCCA 1 cut(s) 255
MseI TTAA 3 cut(s) 354, 1064, 1272
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 255
MspI CCGG 2 cut(s) 42, 521
MspR9I CCNGG 4 cut(s) 43, 174, 522, 1240
MunI CAATTG 2 cut(s) 906, 1580
MvaI CCWGG 2 cut(s) 174, 1240
MwoI GCNNNNNNNGC 3 cut(s) 293, 542, 1192
NarI GGCGCC 1 cut(s) 545
NciI CCSGG 2 cut(s) 43, 522
NcoI CCATGG 1 cut(s) 218
NdeI CATATG 1 cut(s) 1333
NdeII GATC 4 cut(s) 361, 415, 578, 662
NlaIV GGNNCC 3 cut(s) 40, 178, 546
NmuCI GTSAC 2 cut(s) 767, 1242
NsiI ATGCAT 2 cut(s) 1078, 1333
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 1 cut(s) 1178
PagI TCATGA 1 cut(s) 871
PdmI GAANNNNTTC 2 cut(s) 319, 1502
PfeI GAWTC 5 cut(s) 13, 278, 377, 632, 1520
PflMI CCANNNNNTGG 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 2 cut(s) 1260, 1534
PleI GAGTC 3 cut(s) 435, 470, 756
PluTI GGCGCC 1 cut(s) 548
PmaCI CACGTG 1 cut(s) 1541
PmlI CACGTG 1 cut(s) 1541
PpsI GAGTC 3 cut(s) 435, 470, 756
Ppu21I YACGTR 1 cut(s) 1541
PsiI TTATAA 3 cut(s) 516, 597, 843
Psp124BI GAGCTC 1 cut(s) 1431
Psp1406I AACGTT 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 172, 1238
PspCI CACGTG 1 cut(s) 1541
PspGI CCWGG 2 cut(s) 172, 1238
PspN4I GGNNCC 3 cut(s) 40, 178, 546
PspPI GGNCC 2 cut(s) 125, 170
PstI CTGCAG 1 cut(s) 1263
PsuI RGATCY 2 cut(s) 361, 415
RsaI GTAC 5 cut(s) 40, 698, 775, 820, 937
RsaNI GTAC 5 cut(s) 39, 697, 774, 819, 936
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 1431
SalI GTCGAC 1 cut(s) 887
SaqAI TTAA 3 cut(s) 354, 1064, 1272
SatI GCNGC 2 cut(s) 1259, 1533
Sau3AI GATC 4 cut(s) 361, 415, 578, 662
Sau96I GGNCC 2 cut(s) 125, 170
ScaI AGTACT 2 cut(s) 775, 937
SchI GAGTC 3 cut(s) 436, 470, 757
ScrFI CCNGG 4 cut(s) 43, 174, 522, 1240
SduI GDGCHC 2 cut(s) 1431, 1439
SfaNI GCATC 2 cut(s) 523, 1063
SfcI CTRYAG 2 cut(s) 877, 1259
SfoI GGCGCC 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 1178
SinI GGWCC 2 cut(s) 125, 170
SmiMI CAYNNNNRTG 1 cut(s) 623
SspDI GGCGCC 1 cut(s) 544
SspMI CTAG 2 cut(s) 420, 1353
SstI GAGCTC 1 cut(s) 1431
StyD4I CCNGG 4 cut(s) 41, 172, 520, 1238
StyI CCWWGG 2 cut(s) 218, 419
TaaI ACNGT 3 cut(s) 773, 1117, 1408
TaiI ACGT 4 cut(s) 321, 1464, 1543, 1555
TaqI TCGA 3 cut(s) 751, 888, 1178
TatI WGTACW 3 cut(s) 696, 773, 935
TfiI GAWTC 5 cut(s) 13, 278, 377, 632, 1520
Tru1I TTAA 3 cut(s) 354, 1064, 1272
Tru9I TTAA 3 cut(s) 354, 1064, 1272
TscAI CASTG 3 cut(s) 268, 414, 469
TseFI GTSAC 2 cut(s) 767, 1242
TseI GCWGC 2 cut(s) 1258, 1532
Tsp45I GTSAC 2 cut(s) 767, 1242
TspDTI ATGAA 8 cut(s) 106, 312, 607, 828, 1014, 1055, 1350, 1584
TspGWI ACGGA 1 cut(s) 1496
TspRI CASTG 3 cut(s) 268, 414, 469
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 125, 170
XagI CCTNNNNNAGG 1 cut(s) 640
XapI RAATTY 2 cut(s) 301, 1337
XceI RCATGY 1 cut(s) 512
XmaJI CCTAGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 2 cut(s) 319, 1502
XspI CTAG 2 cut(s) 420, 1353
ZrmI AGTACT 2 cut(s) 775, 937
Zsp2I ATGCAT 2 cut(s) 1078, 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.