pycom04g10940

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009698
Physical Location & Seq
Forward (+)
13742244 .. 13745063
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10940.1

Sequence Viewer

Length: 1647 bp
ATGCAATTCCCACGCATAGTGATCGTGCAGGGCGCTCACATCATCTTGTATTCTTTTTTTCCCTCGACTGCAAAATCCAAGTCCCTCTCACTGTCAGATCTGACCAAGATCTCCGTCCTTACATTGTCCGGCGTGTTCTCCGGCCACTTAGGCGCCGGGAAGTTCATCGTTCACCGTCTATGGATCCCACTGTCAGATAAATTAAGTGCAGAAAGTTTGATCTTGGAATTGGAATGTCAATGTGCATTGTTGGTGGTAGGAGATTTGTATATAGGATCTTTTCAGAATCAGGGACTTTGGATGGCAAAGGGTACTGGGTGTTTAAACAAAGACCGTGCTGTGAATTTTGATGAAAGGAGCATTCCGTCTGTTGGACTGGAAAAGATGAATTTGGCTAGTAAAGTTGGTGAGGATTTATTTGGGAATGACTCCTCATTTGGTCTATCTATGTCCAATACACTAGAAGATCCTAGATCCAGTCCTAACTACGGTGGATTTAGAAAAGTCAAAGTTAGCGAGGTAAAGGACTCTGAGAATGTCATGCCTGTTTCAATCGGACAAGCTTATGTTCAGGCAGATAATGATGCTGTGTTGGCAGCTCACGCTTACAAGTCTGACAACAACTCAGCATCCACCGGCCTCATTTATAAGAAGGAGGATGACCACTTCATATCCATGAGTGATACCTACCGCAGGGCAGATAACAGCTTCATTTCAATTGGGCAACCTTTTAACGAGGGGGATGAAAGCATATCGATTGGTCAATCATACAAGGAGAGCAATAACACCCTATCAGTGCGTCAGACATTCAACAAAAGTGACAATAATATCATTTCTATCGGCCAAACCTACAATAAGGCAGAGGAAAGTACCATGTCAACGGGTCATTTCTACTATAAAGGGGAAGAGAGCACTATATCCGTCGGTCATGCCTACAGCAAGGGTGACAACAATATGTTATCGATTGGTCACTCTGATGATAAGCGTGAGAGTACTATCATATCCTTTGGCAGATGTGATGACGATGATGCAAATACCTCTGCAATCTCTGGTTATGAGCTTCTGATGGGTCAACCTTTTTTTAAAACAGAAGCTGTGAATGAGAGAGAGTTGGGTAAATCAAAGTCCTGTGTACTTGTCAATATACCTCATATAACTGCTGGAAATGAAAATGTTTTGAAAAAGAAAGTTGAACAGAAAACGTCTAAGAAGGTTCCCCCGAACAACTTCCCATCAAATGTTCGAAGTTTATTGTCAACGGGTATGCTGGATGGAGTTCCTGTAAAATATACAGCCTGGTCACGGGAGAAGGAGCTGCAGGGTGTGATCAAAGGTTCTGGGTATCTATGTGGCTGTCAGTCGTGCAACTTCTCAAAGGTAATCAATGCATATGAATTTGAGCGTCACGCTGATTGCAAAACAAAACACCCGAATAATCACATATATTTTGAGAATGGCAAAACTATATATGGGATTGTTCAAGAGCTCAGGAGCACACCGCAGAATATGCTGTTTGAAGTTATTCAGACTATTATTGGTTCGCCTATCAATCAGAAATCCTTCCACCTTTGGAAAGAATCCTTTTTGGCAGCAACACGTGAACTTCAGCGTATATATGGCAAGGAAGAGGGAAAGCAACTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

549

Amino Acids

60.62

Weight (kDa)

6.52

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 441 - 495 3e-14 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 152
AciI CCGC 2 cut(s) 691, 1499
AclWI GGATC 5 cut(s) 178, 191, 283, 461, 468
AcoI YGGCCR 2 cut(s) 142, 841
AcsI RAATTY 3 cut(s) 343, 388, 1394
AcuI CTGAAG 1 cut(s) 1589
AcvI CACGTG 1 cut(s) 1598
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 4 cut(s) 313, 871, 994, 1134
AfiI CCNNNNNNNGG 4 cut(s) 371, 488, 693, 1302
AflIII ACRYGT 1 cut(s) 1595
AgsI TTSAA 7 cut(s) 552, 717, 811, 1180, 1193, 1481, 1517
AjnI CCWGG 1 cut(s) 1295
AluBI AGCT 7 cut(s) 563, 599, 708, 1060, 1094, 1315, 1486
AluI AGCT 7 cut(s) 563, 599, 708, 1060, 1094, 1315, 1486
Alw21I GWGCWC 3 cut(s) 914, 1488, 1496
AlwI GGATC 5 cut(s) 178, 191, 283, 461, 468
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 3 cut(s) 142, 637, 841
ApeKI GCWGC 3 cut(s) 596, 1315, 1589
ApoI RAATTY 3 cut(s) 343, 388, 1394
ArsI GACNNNNNNTTYG 4 cut(s) 65, 97, 419, 451
Asp700I GAANNNNTTC 3 cut(s) 1226, 1521, 1559
AspLEI GCGC 2 cut(s) 35, 155
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 3 cut(s) 164, 419, 956
AsuII TTCGAA 1 cut(s) 1243
BamHI GGATCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 1488
BbrPI CACGTG 1 cut(s) 1598
Bbv12I GWGCWC 3 cut(s) 914, 1488, 1496
BbvI GCAGC 3 cut(s) 608, 1302, 1601
BccI CCATC 4 cut(s) 295, 1060, 1240, 1265
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 1 cut(s) 1297
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 1 cut(s) 157
BfaI CTAG 3 cut(s) 396, 461, 471
BfmI CTRYAG 2 cut(s) 934, 1316
BfoI RGCGCY 2 cut(s) 36, 156
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 2 cut(s) 97, 108
BisI GCNGC 3 cut(s) 597, 1316, 1590
BlsI GCNGC 3 cut(s) 598, 1317, 1591
BmcAI AGTACT 1 cut(s) 994
Bme1390I CCNGG 2 cut(s) 157, 1297
BmiI GGNNCC 3 cut(s) 154, 185, 1215
BmrFI CCNGG 2 cut(s) 157, 1297
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 3 cut(s) 574, 638, 1018
BmuI ACTGGG 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 1487
Bpu14I TTCGAA 1 cut(s) 1243
BpuMI CCSGG 1 cut(s) 157
Bsa29I ATCGAT 2 cut(s) 755, 962
BsaAI YACGTR 1 cut(s) 1598
BsaHI GRCGYC 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 1266, 1296
Bsc4I CCNNNNNNNGG 4 cut(s) 371, 488, 693, 1302
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 319, 381, 477
BseBI CCWGG 1 cut(s) 1297
BseCI ATCGAT 2 cut(s) 755, 962
BseGI GGATG 5 cut(s) 306, 629, 664, 748, 1276
BseLI CCNNNNNNNGG 4 cut(s) 371, 488, 693, 1302
BseMII CTCAG 3 cut(s) 522, 639, 1501
BseNI ACTGG 3 cut(s) 319, 381, 477
BseRI GAGGAG 1 cut(s) 421
BseXI GCAGC 3 cut(s) 608, 1302, 1601
BsgI GTGCAG 2 cut(s) 47, 228
BshFI GGCC 3 cut(s) 144, 639, 843
BshNI GGYRCC 1 cut(s) 152
BshVI ATCGAT 2 cut(s) 755, 962
BsiHKAI GWGCWC 3 cut(s) 914, 1488, 1496
BsiSI CCGG 4 cut(s) 129, 141, 156, 636
BslFI GGGAC 2 cut(s) 67, 306
BslI CCNNNNNNNGG 4 cut(s) 371, 488, 693, 1302
BsmFI GGGAC 2 cut(s) 67, 306
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 3 cut(s) 144, 639, 843
Bsp119I TTCGAA 1 cut(s) 1243
Bsp1286I GDGCHC 3 cut(s) 914, 1488, 1496
Bsp143I GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
BspACI CCGC 2 cut(s) 691, 1499
BspANI GGCC 3 cut(s) 144, 639, 843
BspCNI CTCAG 3 cut(s) 523, 638, 1500
BspDI ATCGAT 2 cut(s) 755, 962
BspHI TCATGA 1 cut(s) 1643
BspLI GGNNCC 3 cut(s) 154, 185, 1215
BspMAI CTGCAG 1 cut(s) 1320
BspPI GGATC 5 cut(s) 178, 191, 283, 461, 468
BspT104I TTCGAA 1 cut(s) 1243
BspT107I GGYRCC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 3 cut(s) 319, 381, 477
BssAI RCCGGY 1 cut(s) 635
BssMI GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
BssNI GRCGYC 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 1297
Bst4CI ACNGT 5 cut(s) 93, 176, 192, 335, 491
Bst6I CTCTTC 2 cut(s) 900, 1620
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 1507
BstBAI YACGTR 1 cut(s) 1598
BstBI TTCGAA 1 cut(s) 1243
BstDEI CTNAG 5 cut(s) 148, 531, 625, 1206, 1487
BstENI CCTNNNNNAGG 1 cut(s) 691
BstF5I GGATG 5 cut(s) 306, 629, 664, 748, 1276
BstH2I RGCGCY 2 cut(s) 36, 156
BstHHI GCGC 2 cut(s) 35, 155
BstKTI GATC 9 cut(s) 24, 100, 111, 186, 222, 278, 469, 476, 1329
BstMBI GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
BstMWI GCNNNNNNNGC 4 cut(s) 150, 593, 602, 1507
BstNI CCWGG 1 cut(s) 1297
BstSCI CCNGG 2 cut(s) 155, 1295
BstSFI CTRYAG 2 cut(s) 934, 1316
BstV1I GCAGC 3 cut(s) 608, 1302, 1601
BstX2I RGATCY 6 cut(s) 97, 108, 183, 275, 466, 473
BstYI RGATCY 6 cut(s) 97, 108, 183, 275, 466, 473
Bsu15I ATCGAT 2 cut(s) 755, 962
BsuRI GGCC 3 cut(s) 144, 639, 843
BsuTUI ATCGAT 2 cut(s) 755, 962
BtsCI GGATG 5 cut(s) 306, 629, 664, 748, 1276
BtsIMutI CAGTG 3 cut(s) 89, 188, 801
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 1 cut(s) 1643
CfoI GCGC 2 cut(s) 35, 155
Cfr10I RCCGGY 1 cut(s) 635
ClaI ATCGAT 2 cut(s) 755, 962
CseI GACGC 2 cut(s) 788, 1391
Csp6I GTAC 4 cut(s) 312, 870, 993, 1133
CviAII CATG 5 cut(s) 541, 676, 874, 929, 1644
CviQI GTAC 4 cut(s) 312, 870, 993, 1133
DdeI CTNAG 5 cut(s) 148, 531, 625, 1206, 1487
DinI GGCGCC 1 cut(s) 154
DpnI GATC 9 cut(s) 23, 99, 110, 185, 221, 277, 468, 475, 1328
DpnII GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
DraI TTTAAA 2 cut(s) 324, 1084
EaeI YGGCCR 2 cut(s) 142, 841
Eam1104I CTCTTC 2 cut(s) 900, 1620
EarI CTCTTC 2 cut(s) 900, 1620
Ecl136II GAGCTC 1 cut(s) 1486
Eco24I GRGCYC 1 cut(s) 1488
Eco53kI GAGCTC 1 cut(s) 1486
Eco57I CTGAAG 1 cut(s) 1589
Eco72I CACGTG 1 cut(s) 1598
EcoICRI GAGCTC 1 cut(s) 1486
EcoNI CCTNNNNNAGG 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 1295
EcoT22I ATGCAT 1 cut(s) 1390
EcoT38I GRGCYC 1 cut(s) 1488
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaeI CATG 5 cut(s) 544, 679, 877, 932, 1647
FaqI GGGAC 2 cut(s) 67, 306
FatI CATG 5 cut(s) 540, 675, 873, 928, 1643
FauNDI CATATG 1 cut(s) 1390
FbaI TGATCA 1 cut(s) 1326
Fnu4HI GCNGC 3 cut(s) 597, 1316, 1590
FokI GGATG 5 cut(s) 313, 616, 671, 755, 1283
FriOI GRGCYC 1 cut(s) 1488
Fsp4HI GCNGC 3 cut(s) 597, 1316, 1590
FspBI CTAG 3 cut(s) 396, 461, 471
GlaI GCGC 2 cut(s) 34, 154
GluI GCNGC 3 cut(s) 597, 1316, 1590
HaeII RGCGCY 2 cut(s) 36, 156
HaeIII GGCC 3 cut(s) 144, 639, 843
HapII CCGG 4 cut(s) 129, 141, 156, 636
HgaI GACGC 2 cut(s) 788, 1391
HhaI GCGC 2 cut(s) 35, 155
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 5 cut(s) 544, 679, 877, 932, 1647
Hin6I GCGC 2 cut(s) 33, 153
HinP1I GCGC 2 cut(s) 33, 153
HincII GTYRAC 3 cut(s) 879, 1073, 1257
HindII GTYRAC 3 cut(s) 879, 1073, 1257
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 4 cut(s) 286, 428, 527, 1577
HpaII CCGG 4 cut(s) 129, 141, 156, 636
HphI GGTGA 3 cut(s) 164, 419, 956
Hpy166II GTNNAC 6 cut(s) 172, 879, 1073, 1133, 1257, 1601
Hpy188III TCNNGA 3 cut(s) 1481, 1489, 1644
Hpy8I GTNNAC 6 cut(s) 172, 879, 1073, 1133, 1257, 1601
Hpy99I CGWCG 1 cut(s) 926
HpyAV CCTTC 4 cut(s) 646, 1204, 1303, 1570
HpyCH4III ACNGT 5 cut(s) 93, 176, 192, 335, 491
HpyCH4IV ACGT 2 cut(s) 1202, 1597
HpyF10VI GCNNNNNNNGC 4 cut(s) 150, 593, 602, 1507
HpyF3I CTNAG 5 cut(s) 148, 531, 625, 1206, 1487
HpySE526I ACGT 2 cut(s) 1202, 1597
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 5 cut(s) 544, 679, 877, 932, 1647
HspAI GCGC 2 cut(s) 33, 153
KasI GGCGCC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
LmnI GCTCC 3 cut(s) 357, 1312, 1491
Lsp1109I GCAGC 3 cut(s) 608, 1302, 1601
LweI GCATC 3 cut(s) 574, 638, 1018
MaeI CTAG 3 cut(s) 396, 461, 471
MaeII ACGT 2 cut(s) 1202, 1597
MaeIII GTNAC 5 cut(s) 818, 944, 968, 1299, 1403
MalI GATC 9 cut(s) 23, 99, 110, 185, 221, 277, 468, 475, 1328
MboI GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
MboII GAAGA 3 cut(s) 476, 917, 1637
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 6 cut(s) 97, 108, 183, 275, 466, 473
MhlI GDGCHC 3 cut(s) 914, 1488, 1496
MluCI AATT 7 cut(s) 5, 200, 227, 343, 388, 717, 1394
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 2 cut(s) 422, 521
MmeI TCCRAC 1 cut(s) 352
Mph1103I ATGCAT 1 cut(s) 1390
MroXI GAANNNNTTC 3 cut(s) 1226, 1521, 1559
MseI TTAA 4 cut(s) 203, 323, 732, 1083
MslI CAYNNNNRTG 2 cut(s) 674, 975
MspI CCGG 4 cut(s) 129, 141, 156, 636
MspR9I CCNGG 2 cut(s) 157, 1297
MssI GTTTAAAC 1 cut(s) 324
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 360
MvaI CCWGG 1 cut(s) 1297
MwoI GCNNNNNNNGC 4 cut(s) 150, 593, 602, 1507
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 157
NdeI CATATG 1 cut(s) 1390
NdeII GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
NlaIII CATG 5 cut(s) 544, 679, 877, 932, 1647
NlaIV GGNNCC 3 cut(s) 154, 185, 1215
NmuCI GTSAC 5 cut(s) 818, 944, 968, 1299, 1403
NsiI ATGCAT 1 cut(s) 1390
NspV TTCGAA 1 cut(s) 1243
PagI TCATGA 1 cut(s) 1643
PctI GAATGC 1 cut(s) 360
PdmI GAANNNNTTC 3 cut(s) 1226, 1521, 1559
PfeI GAWTC 2 cut(s) 286, 1577
PkrI GCNGC 3 cut(s) 598, 1317, 1591
PleI GAGTC 2 cut(s) 422, 521
PluTI GGCGCC 1 cut(s) 156
PmaCI CACGTG 1 cut(s) 1598
PmeI GTTTAAAC 1 cut(s) 324
PmlI CACGTG 1 cut(s) 1598
PpsI GAGTC 2 cut(s) 422, 521
Ppu21I YACGTR 1 cut(s) 1598
PsiI TTATAA 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 1488
Psp6I CCWGG 1 cut(s) 1295
PspCI CACGTG 1 cut(s) 1598
PspGI CCWGG 1 cut(s) 1295
PspN4I GGNNCC 3 cut(s) 154, 185, 1215
PstI CTGCAG 1 cut(s) 1320
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 6 cut(s) 97, 108, 183, 275, 466, 473
RsaI GTAC 4 cut(s) 313, 871, 994, 1134
RsaNI GTAC 4 cut(s) 312, 870, 993, 1133
RseI CAYNNNNRTG 2 cut(s) 674, 975
SacI GAGCTC 1 cut(s) 1488
SaqAI TTAA 4 cut(s) 203, 323, 732, 1083
SatI GCNGC 3 cut(s) 597, 1316, 1590
Sau3AI GATC 9 cut(s) 21, 97, 108, 183, 219, 275, 466, 473, 1326
ScaI AGTACT 1 cut(s) 994
SchI GAGTC 2 cut(s) 422, 521
ScrFI CCNGG 2 cut(s) 157, 1297
SduI GDGCHC 3 cut(s) 914, 1488, 1496
SfaNI GCATC 3 cut(s) 574, 638, 1018
SfcI CTRYAG 2 cut(s) 934, 1316
SfoI GGCGCC 1 cut(s) 154
SfuI TTCGAA 1 cut(s) 1243
SmiMI CAYNNNNRTG 2 cut(s) 674, 975
Sse9I AATT 7 cut(s) 5, 200, 227, 343, 388, 717, 1394
SsiI CCGC 2 cut(s) 691, 1499
SspDI GGCGCC 1 cut(s) 152
SspMI CTAG 3 cut(s) 396, 461, 471
SstI GAGCTC 1 cut(s) 1488
StyD4I CCNGG 2 cut(s) 155, 1295
TaaI ACNGT 5 cut(s) 93, 176, 192, 335, 491
TaiI ACGT 2 cut(s) 1205, 1600
TaqI TCGA 4 cut(s) 65, 755, 962, 1243
TaqII GACCGA 1 cut(s) 914
TasI AATT 7 cut(s) 5, 200, 227, 343, 388, 717, 1394
TatI WGTACW 2 cut(s) 992, 1132
TfiI GAWTC 2 cut(s) 286, 1577
Tru1I TTAA 4 cut(s) 203, 323, 732, 1083
Tru9I TTAA 4 cut(s) 203, 323, 732, 1083
TscAI CASTG 3 cut(s) 96, 195, 801
TseFI GTSAC 5 cut(s) 818, 944, 968, 1299, 1403
TseI GCWGC 3 cut(s) 596, 1315, 1589
Tsp45I GTSAC 5 cut(s) 818, 944, 968, 1299, 1403
TspDTI ATGAA 9 cut(s) 154, 366, 401, 658, 700, 759, 1182, 1407, 1632
TspGWI ACGGA 3 cut(s) 103, 354, 910
TspRI CASTG 3 cut(s) 96, 195, 801
XagI CCTNNNNNAGG 1 cut(s) 691
XapI RAATTY 3 cut(s) 343, 388, 1394
XmnI GAANNNNTTC 3 cut(s) 1226, 1521, 1559
XspI CTAG 3 cut(s) 396, 461, 471
ZrmI AGTACT 1 cut(s) 994
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.