Rw3G012860

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009698
Physical Location & Seq
Reverse (-)
12614646 .. 12616912
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G012860.1

Sequence Viewer

Length: 1587 bp
ATGTCTTTTCAGAATCAGGGCTTTTGGATGGCAAAGGGTACCGGGGGTGTAAATGATGGTGAGAGGCTTTATGATAATTCTTCTGCAACTGAGCCTAAGCGTTCTCATCAATGGTTCATGGATGGTCCTGAGGTAGAGTTATTCCCAAACAAGAAACAGGCTATTGAAGTTCCAGGCACCAATCTATTTTCAGGCTTGTTGAACGCAAATGTTTCTCCATGGGGAAATGTTCCCAGTTTTCAGTCATTCTCTGGCCAGTTCACTGAAAGGTTATTTGATTCTGAAGCAGACAGAGCTGTGAATTTTGAGGACAGAAACATTTCATCTGTTGGCTCAGAAAAAATGAGAAAAATTAATGAGGATCTATTTGGGAATGATTCTTCTTTTGGTTTATCTATGTCCCATTCACTGGAAGATCCTAGGTTGAGTCCTAACTATGGTGGTTTTAGAAAAGTGAAAGTCAGTGAGGTAAAGGACTCTGAGAATGTCATGCGTGTTTCGGTTGGACATGCTTATAATCCGGGAGATAACGATGCCATGTTGGCACCTCATGTTTACAAGGCGGATGACAACTCAGGATCAATGGGTCTCACTTATAAGAAAGAGGATGAGAACTTCACAACCATGAGTGATTCCTATAATAGGGCAGATAACAATTTTAGATCAATGGGACACTTTACTAAAGGGGATGAGTACATAGGAATTGGTCAAACATACAAGGAAAATAGTAATACCCTTTTGAGGAGTCGAACATTCAACAAGGGTGACAGCACTATCATCTCGGTTGGTGAAACCTCCAACAAGGCAGATGAAAGTACTATGCCAACTGGTCATATTTATAGTAAAGGGGAAGAGATATCAATGGGTCATGACTATAGCAAGGTCGACAATAATATGTTGTCAATTGGTCACTCTTACAATAAGGGAGAGAGTACCATCATATCCTTTGGCGGGTGTGATGATGATGATGTAAATACCTCAACAATCTCTGGCTATGAACTTCTTATGGGTCAATCTTTTCCAAAAACAGAAGCTATGAATGAGAAAGACTTGGGTAAATTAAATGCTGATGCATTTGTAAATCAACCTCATATAATTGCTGGAGCTGAAACTGTTTCCAGAAAGAAGGATGACCAGAAAATGTCTAAGAAGTCGCCTTCAAACAACTTCCCTTCGAATGTGCGAAGTTTGCTATCAACTGGTATGCTGGATGGAGTTCCTGTAAAGTATATTGCCTGGTCACGGGAGAAGGAGCTGCAGGCTGTAATTAAAGGTTCTGGGTATCTATGTGGCTGTCAGTCATGTAACTTCTCTAAGGTAATCAATGCATATGAATTTGAGCGTCATGCTAGTTGCAAAACAAAACACCCAAACAACCACATATATTTTGAGAATGGGAAGACCATCTATGGGATTGTGCAAGAGCTCAGGAGCACACCTCAGAATATGCTGTTTGACGTTATTCAGACTATTACTGGTTCACAAATCAATCAGAAATCCTTCCGTCTTTGGAAAGAATCGTTTTTGGCAGCAACACGTGAACTTCAACGTATATACGGCAAGGATGAAGTGAAACAATTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

528

Amino Acids

58.87

Weight (kDa)

5.82

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 422 - 475 2.7e-13 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 516, 597
Acc65I GGTACC 1 cut(s) 38
AccB1I GGYRCC 3 cut(s) 38, 176, 544
AccB7I CCANNNNNTGG 1 cut(s) 409
AccI GTMKAC 1 cut(s) 885
AciI CCGC 2 cut(s) 563, 951
AclWI GGATC 3 cut(s) 369, 410, 586
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 301, 1334
AcuI CTGAAG 1 cut(s) 303
AcvI CACGTG 1 cut(s) 1538
AfaI GTAC 4 cut(s) 40, 695, 817, 934
AfiI CCNNNNNNNGG 7 cut(s) 409, 437, 642, 741, 951, 1242, 1410
AflIII ACRYGT 1 cut(s) 1535
AgsI TTSAA 5 cut(s) 167, 202, 757, 1161, 1547
AjnI CCWGG 2 cut(s) 172, 1235
AluBI AGCT 5 cut(s) 296, 1034, 1106, 1255, 1426
AluI AGCT 5 cut(s) 296, 1034, 1106, 1255, 1426
Alw21I GWGCWC 2 cut(s) 1428, 1436
Alw26I GTCTC 1 cut(s) 593
AlwI GGATC 3 cut(s) 369, 410, 586
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 1255, 1529
ApoI RAATTY 2 cut(s) 301, 1334
AseI ATTAAT 1 cut(s) 354
Asp700I GAANNNNTTC 2 cut(s) 319, 1499
Asp718I GGTACC 1 cut(s) 38
AspA2I CCTAGG 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 125
AsuC2I CCSGG 2 cut(s) 43, 522
AsuHPI GGTGA 3 cut(s) 71, 776, 800
AsuII TTCGAA 1 cut(s) 1175
AvaII GGWCC 1 cut(s) 125
AvrII CCTAGG 1 cut(s) 419
AxyI CCTNAGG 1 cut(s) 129
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 3 cut(s) 38, 176, 544
BanII GRGCYC 1 cut(s) 1428
BbrPI CACGTG 1 cut(s) 1538
BbsI GAAGAC 1 cut(s) 1406
Bbv12I GWGCWC 2 cut(s) 1428, 1436
BbvI GCAGC 2 cut(s) 1242, 1541
BccI CCATC 6 cut(s) 22, 50, 116, 944, 1205, 1412
BceAI ACGGC 1 cut(s) 1573
BciT130I CCWGG 2 cut(s) 174, 1237
BcnI CCSGG 2 cut(s) 43, 522
BcoDI GTCTC 1 cut(s) 593
BfaI CTAG 2 cut(s) 420, 1350
BfmI CTRYAG 2 cut(s) 874, 1256
BglI GCCNNNNNGGC 1 cut(s) 542
BisI GCNGC 2 cut(s) 1256, 1530
BlnI CCTAGG 1 cut(s) 419
BlsI GCNGC 2 cut(s) 1257, 1531
BmcAI AGTACT 1 cut(s) 817
Bme1390I CCNGG 4 cut(s) 43, 174, 522, 1237
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 3 cut(s) 40, 178, 546
BmrFI CCNGG 4 cut(s) 43, 174, 522, 1237
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 2 cut(s) 523, 1060
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 1406
BplI GAGNNNNNCTC 2 cut(s) 1423, 1455
BpmI CTGGAG 1 cut(s) 1122
Bpu10I CCTNAGC 2 cut(s) 96, 1427
Bpu14I TTCGAA 1 cut(s) 1175
BpuMI CCSGG 2 cut(s) 43, 522
BsaAI YACGTR 1 cut(s) 1538
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 42, 218, 419
BsaXI ACNNNNNCTCC 2 cut(s) 1206, 1236
Bsc4I CCNNNNNNNGG 7 cut(s) 409, 437, 642, 741, 951, 1242, 1410
Bse1I ACTGG 6 cut(s) 234, 256, 414, 832, 1204, 1480
Bse21I CCTNAGG 1 cut(s) 129
BseBI CCWGG 2 cut(s) 174, 1237
BseDI CCNNGG 3 cut(s) 42, 218, 419
BseGI GGATG 8 cut(s) 33, 127, 571, 613, 694, 1135, 1216, 1570
BseLI CCNNNNNNNGG 7 cut(s) 409, 437, 642, 741, 951, 1242, 1410
BseMII CTCAG 7 cut(s) 81, 120, 348, 471, 588, 1441, 1454
BseNI ACTGG 6 cut(s) 234, 256, 414, 832, 1204, 1480
BseRI GAGGAG 1 cut(s) 757
BseXI GCAGC 2 cut(s) 1242, 1541
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 3 cut(s) 38, 176, 544
BsiHKAI GWGCWC 2 cut(s) 1428, 1436
BsiSI CCGG 2 cut(s) 42, 521
BslFI GGGAC 2 cut(s) 385, 684
BslI CCNNNNNNNGG 7 cut(s) 409, 437, 642, 741, 951, 1242, 1410
BsmAI GTCTC 1 cut(s) 593
BsmFI GGGAC 2 cut(s) 385, 684
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 1175
Bsp1286I GDGCHC 2 cut(s) 1428, 1436
Bsp143I GATC 4 cut(s) 361, 415, 578, 662
Bsp19I CCATGG 1 cut(s) 218
BspACI CCGC 2 cut(s) 563, 951
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 7 cut(s) 82, 121, 347, 472, 587, 1440, 1453
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 3 cut(s) 40, 178, 546
BspMAI CTGCAG 1 cut(s) 1260
BspPI GGATC 3 cut(s) 369, 410, 586
BspT104I TTCGAA 1 cut(s) 1175
BspT107I GGYRCC 3 cut(s) 38, 176, 544
BspTNI GGTCTC 1 cut(s) 593
BsrI ACTGG 6 cut(s) 234, 256, 414, 832, 1204, 1480
BssECI CCNNGG 3 cut(s) 42, 218, 419
BssMI GATC 4 cut(s) 361, 415, 578, 662
BssT1I CCWWGG 2 cut(s) 218, 419
Bst2UI CCWGG 2 cut(s) 174, 1237
Bst4CI ACNGT 1 cut(s) 1114
Bst6I CTCTTC 1 cut(s) 846
BstBAI YACGTR 1 cut(s) 1538
BstBI TTCGAA 1 cut(s) 1175
BstC8I GCNNGC 1 cut(s) 1260
BstDSI CCRYGG 1 cut(s) 218
BstENI CCTNNNNNAGG 1 cut(s) 640
BstF5I GGATG 8 cut(s) 33, 127, 571, 613, 694, 1135, 1216, 1570
BstKTI GATC 4 cut(s) 364, 418, 581, 665
BstMAI GTCTC 1 cut(s) 593
BstMBI GATC 4 cut(s) 361, 415, 578, 662
BstMWI GCNNNNNNNGC 3 cut(s) 293, 542, 1189
BstNI CCWGG 2 cut(s) 174, 1237
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 4 cut(s) 41, 172, 520, 1235
BstSFI CTRYAG 2 cut(s) 874, 1256
BstV1I GCAGC 2 cut(s) 1242, 1541
BstV2I GAAGAC 1 cut(s) 1406
BstX2I RGATCY 2 cut(s) 361, 415
BstYI RGATCY 2 cut(s) 361, 415
Bsu36I CCTNAGG 1 cut(s) 129
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 218
BtsCI GGATG 8 cut(s) 33, 127, 571, 613, 694, 1135, 1216, 1570
BtsIMutI CAGTG 3 cut(s) 261, 407, 469
Cac8I GCNNGC 1 cut(s) 1260
CciI TCATGA 1 cut(s) 868
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 1 cut(s) 1331
Csp6I GTAC 4 cut(s) 39, 694, 816, 933
CviQI GTAC 4 cut(s) 39, 694, 816, 933
DpnI GATC 4 cut(s) 363, 417, 580, 664
DpnII GATC 4 cut(s) 361, 415, 578, 662
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
EciI GGCGGA 1 cut(s) 578
Ecl136II GAGCTC 1 cut(s) 1426
Eco130I CCWWGG 2 cut(s) 218, 419
Eco24I GRGCYC 1 cut(s) 1428
Eco31I GGTCTC 1 cut(s) 593
Eco32I GATATC 1 cut(s) 858
Eco47I GGWCC 1 cut(s) 125
Eco53kI GAGCTC 1 cut(s) 1426
Eco57I CTGAAG 1 cut(s) 303
Eco72I CACGTG 1 cut(s) 1538
Eco81I CCTNAGG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 1426
EcoNI CCTNNNNNAGG 1 cut(s) 640
EcoRII CCWGG 2 cut(s) 172, 1235
EcoRV GATATC 1 cut(s) 858
EcoT14I CCWWGG 2 cut(s) 218, 419
EcoT22I ATGCAT 2 cut(s) 1075, 1330
EcoT38I GRGCYC 1 cut(s) 1428
ErhI CCWWGG 2 cut(s) 218, 419
FaqI GGGAC 2 cut(s) 385, 684
FauI CCCGC 1 cut(s) 944
FauNDI CATATG 1 cut(s) 1330
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 2 cut(s) 1256, 1530
FokI GGATG 8 cut(s) 40, 134, 578, 620, 701, 1142, 1223, 1577
FriOI GRGCYC 1 cut(s) 1428
Fsp4HI GCNGC 2 cut(s) 1256, 1530
FspBI CTAG 2 cut(s) 420, 1350
GluI GCNGC 2 cut(s) 1256, 1530
GsuI CTGGAG 1 cut(s) 1122
HaeIII GGCC 1 cut(s) 255
HapII CCGG 2 cut(s) 42, 521
HgaI GACGC 1 cut(s) 1331
HincII GTYRAC 1 cut(s) 886
HindII GTYRAC 1 cut(s) 886
HinfI GANTC 8 cut(s) 13, 278, 377, 427, 476, 632, 745, 1517
HpaII CCGG 2 cut(s) 42, 521
HphI GGTGA 3 cut(s) 71, 776, 800
Hpy166II GTNNAC 5 cut(s) 261, 556, 886, 1481, 1541
Hpy188I TCNGA 7 cut(s) 12, 283, 337, 481, 1443, 1467, 1494
Hpy188III TCNNGA 5 cut(s) 128, 576, 869, 1119, 1429
Hpy8I GTNNAC 5 cut(s) 261, 556, 886, 1481, 1541
HpyAV CCTTC 5 cut(s) 1120, 1167, 1182, 1243, 1510
HpyCH4III ACNGT 1 cut(s) 1114
HpyCH4IV ACGT 3 cut(s) 1458, 1537, 1549
HpyCH4V TGCA 6 cut(s) 86, 1073, 1258, 1328, 1356, 1420
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 542, 1189
HpySE526I ACGT 3 cut(s) 1458, 1537, 1549
KpnI GGTACC 1 cut(s) 42
Kzo9I GATC 4 cut(s) 361, 415, 578, 662
LmnI GCTCC 3 cut(s) 1103, 1252, 1431
Lsp1109I GCAGC 2 cut(s) 1242, 1541
LweI GCATC 2 cut(s) 523, 1060
MaeI CTAG 2 cut(s) 420, 1350
MaeII ACGT 3 cut(s) 1458, 1537, 1549
MaeIII GTNAC 4 cut(s) 764, 908, 1239, 1304
MalI GATC 4 cut(s) 363, 417, 580, 664
MboI GATC 4 cut(s) 361, 415, 578, 662
MboII GAAGA 5 cut(s) 72, 372, 425, 863, 1411
MfeI CAATTG 2 cut(s) 903, 1577
MflI RGATCY 2 cut(s) 361, 415
MhlI GDGCHC 2 cut(s) 1428, 1436
MlsI TGGCCA 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 3 cut(s) 436, 470, 754
MmeI TCCRAC 2 cut(s) 484, 822
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 2 cut(s) 1075, 1330
MroXI GAANNNNTTC 2 cut(s) 319, 1499
MscI TGGCCA 1 cut(s) 255
MseI TTAA 3 cut(s) 354, 1061, 1269
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 255
MspI CCGG 2 cut(s) 42, 521
MspR9I CCNGG 4 cut(s) 43, 174, 522, 1237
MunI CAATTG 2 cut(s) 903, 1577
MvaI CCWGG 2 cut(s) 174, 1237
MwoI GCNNNNNNNGC 3 cut(s) 293, 542, 1189
NciI CCSGG 2 cut(s) 43, 522
NcoI CCATGG 1 cut(s) 218
NdeI CATATG 1 cut(s) 1330
NdeII GATC 4 cut(s) 361, 415, 578, 662
NlaIV GGNNCC 3 cut(s) 40, 178, 546
NmuCI GTSAC 3 cut(s) 764, 908, 1239
NsiI ATGCAT 2 cut(s) 1075, 1330
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 1 cut(s) 1175
PagI TCATGA 1 cut(s) 868
PdmI GAANNNNTTC 2 cut(s) 319, 1499
PfeI GAWTC 5 cut(s) 13, 278, 377, 632, 1517
PflMI CCANNNNNTGG 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 2 cut(s) 1257, 1531
PleI GAGTC 3 cut(s) 435, 470, 753
PmaCI CACGTG 1 cut(s) 1538
PmlI CACGTG 1 cut(s) 1538
PpsI GAGTC 3 cut(s) 435, 470, 753
Ppu21I YACGTR 1 cut(s) 1538
PshBI ATTAAT 1 cut(s) 354
PsiI TTATAA 2 cut(s) 516, 597
Psp124BI GAGCTC 1 cut(s) 1428
Psp6I CCWGG 2 cut(s) 172, 1235
PspCI CACGTG 1 cut(s) 1538
PspGI CCWGG 2 cut(s) 172, 1235
PspN4I GGNNCC 3 cut(s) 40, 178, 546
PspPI GGNCC 1 cut(s) 125
PstI CTGCAG 1 cut(s) 1260
PsuI RGATCY 2 cut(s) 361, 415
RsaI GTAC 4 cut(s) 40, 695, 817, 934
RsaNI GTAC 4 cut(s) 39, 694, 816, 933
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 1428
SalI GTCGAC 1 cut(s) 884
SaqAI TTAA 3 cut(s) 354, 1061, 1269
SatI GCNGC 2 cut(s) 1256, 1530
Sau3AI GATC 4 cut(s) 361, 415, 578, 662
Sau96I GGNCC 1 cut(s) 125
ScaI AGTACT 1 cut(s) 817
SchI GAGTC 3 cut(s) 436, 470, 754
ScrFI CCNGG 4 cut(s) 43, 174, 522, 1237
SduI GDGCHC 2 cut(s) 1428, 1436
SfaNI GCATC 2 cut(s) 523, 1060
SfcI CTRYAG 2 cut(s) 874, 1256
SfuI TTCGAA 1 cut(s) 1175
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 623
SsiI CCGC 2 cut(s) 563, 951
SspMI CTAG 2 cut(s) 420, 1350
SstI GAGCTC 1 cut(s) 1428
StyD4I CCNGG 4 cut(s) 41, 172, 520, 1235
StyI CCWWGG 2 cut(s) 218, 419
TaaI ACNGT 1 cut(s) 1114
TaiI ACGT 3 cut(s) 1461, 1540, 1552
TaqI TCGA 3 cut(s) 748, 885, 1175
TatI WGTACW 2 cut(s) 693, 815
TfiI GAWTC 5 cut(s) 13, 278, 377, 632, 1517
Tru1I TTAA 3 cut(s) 354, 1061, 1269
Tru9I TTAA 3 cut(s) 354, 1061, 1269
TscAI CASTG 3 cut(s) 268, 414, 469
TseFI GTSAC 3 cut(s) 764, 908, 1239
TseI GCWGC 2 cut(s) 1255, 1529
Tsp45I GTSAC 3 cut(s) 764, 908, 1239
TspDTI ATGAA 7 cut(s) 106, 312, 825, 1011, 1052, 1347, 1581
TspGWI ACGGA 1 cut(s) 1493
TspRI CASTG 3 cut(s) 268, 414, 469
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 125
VspI ATTAAT 1 cut(s) 354
XagI CCTNNNNNAGG 1 cut(s) 640
XapI RAATTY 2 cut(s) 301, 1334
XceI RCATGY 1 cut(s) 512
XmaJI CCTAGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 319, 1499
XspI CTAG 2 cut(s) 420, 1350
ZrmI AGTACT 1 cut(s) 817
Zsp2I ATGCAT 2 cut(s) 1075, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.