Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0016878

Physical Location & Seq

Genomic Coordinates Forward (+)

21232704 .. 21233288

Gene Structure 585 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1125800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 585 bp

ATGGACGACGGCGGACAGCACCATGGATTTAAACTCACCCCTGAACTCATCGGCCTAATGGGCGTTGCAGCCGGAGCCATAATCGTCGCGACGTACCAATGCCTTGTCAACCACATTTGTTGCAGCCAAATGCGCGTAGAATACAACATACAACAAGAACAAACACAAAATGAACATGAACAGCGTCTTCAAGGGCATCACGTCAACCAAAACCTCAGATCAACAACTGCACACAGCGTTAACTCGCCCTCCTACGATCAGTTGATCCCGGTTTTCAAGTACACCAAGGAATGCAGAGAAGGAACCTGCGCCGTTTGTTTGTGTGAATTTACCGACGGCGAGAACATCCGTTTACTGCCGGAATGTGTGCACTTGTTTCATGTGGGGTGCATTGACATGTGGCTCAGCTCACACTCTAACTGCCCTCTTTGTCGAACTGATATTATTCCTAGACGAGCACCGCAGCACGTTGTTCCTCCTCCGCAAGATGTTGTGACTCCTCCTCCGCAGAATACTTTGCCTTCACCACAACATGTTGTGATATTGATGCCAAATTTCGGCGCGGTTCATAATATGAGTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

195

Amino Acids

21.67

Weight (kDa)

6.07

Isoelectric Point (pI)

58.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	98 - 145	2.2e-07	RING-H2 zinc finger domain
zf-RING_2	PF13639	102 - 145	3.8e-13	Ring finger domain
zf-RING_11	PF17123	103 - 131	2.6e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	103 - 144	4.8e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016878)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g14040
malus_domestica	MD04G1125800.v1.1 MD12G1078800.v1.1
prunus_persica	Prupe.6G252700_v2.0.a1 Prupe.6G252800_v2.0.a1 Prupe.6G252900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0466921
rosa_laevigata	RLG00000024545
rosa_multiflora	Rmu_sc0017982.1_g000001 Rmu_sc0023775.1_g000001 Rmu_sc0023776.1_g000002
rosa_roxburghii	Rroxscaffold_6G00414060
rosa_rugosa	Rorug03G0084200
rosa_wichuraiana	Rw3G012480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	314
AccII	CGCG	3 cut(s)	89, 135, 563
AciI	CCGC	5 cut(s)	12, 461, 482, 506, 563
AclWI	GGATC	1 cut(s)	259
AcsI	RAATTY	2 cut(s)	326, 553
AfaI	GTAC	3 cut(s)	95, 281, 580
AfiI	CCNNNNNNNGG	1 cut(s)	557
AflIII	ACRYGT	2 cut(s)	396, 532
AgsI	TTSAA	2 cut(s)	191, 277
AjiI	CACGTC	1 cut(s)	202
AluBI	AGCT	1 cut(s)	408
AluI	AGCT	1 cut(s)	408
Alw21I	GWGCWC	2 cut(s)	372, 460
Alw44I	GTGCAC	1 cut(s)	368
AlwI	GGATC	1 cut(s)	259
AoxI	GGCC	1 cut(s)	52
ApaLI	GTGCAC	1 cut(s)	368
ApeKI	GCWGC	3 cut(s)	68, 123, 463
ApoI	RAATTY	2 cut(s)	326, 553
AspLEI	GCGC	3 cut(s)	135, 311, 563
AsuC2I	CCSGG	1 cut(s)	269
AsuHPI	GGTGA	2 cut(s)	28, 516
BaeGI	GKGCMC	1 cut(s)	372
BbsI	GAAGAC	1 cut(s)	179
Bbv12I	GWGCWC	2 cut(s)	372, 460
BbvI	GCAGC	3 cut(s)	80, 135, 475
BceAI	ACGGC	3 cut(s)	25, 296, 352
BcnI	CCSGG	1 cut(s)	269
BfaI	CTAG	1 cut(s)	450
BfuAI	ACCTGC	1 cut(s)	314
BglI	GCCNNNNNGGC	1 cut(s)	60
BisI	GCNGC	3 cut(s)	69, 124, 464
BlpI	GCTNAGC	1 cut(s)	404
BlsI	GCNGC	3 cut(s)	70, 125, 465
BmcAI	AGTACT	1 cut(s)	580
Bme1390I	CCNGG	1 cut(s)	269
BmgBI	CACGTC	1 cut(s)	202
BmiI	GGNNCC	2 cut(s)	76, 304
BmrFI	CCNGG	1 cut(s)	269
BmsI	GCATC	2 cut(s)	205, 537
BpiI	GAAGAC	1 cut(s)	179
Bpu1102I	GCTNAGC	1 cut(s)	404
BpuMI	CCSGG	1 cut(s)	269
BsaJI	CCNNGG	2 cut(s)	22, 285
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 487, 517
Bsc4I	CCNNNNNNNGG	1 cut(s)	557
BseDI	CCNNGG	2 cut(s)	22, 285
BseGI	GGATG	1 cut(s)	345
BseLI	CCNNNNNNNGG	1 cut(s)	557
BseMII	CTCAG	2 cut(s)	229, 418
BseRI	GAGGAG	3 cut(s)	468, 489, 492
BseSI	GKGCMC	1 cut(s)	372
BseXI	GCAGC	3 cut(s)	80, 135, 475
BsgI	GTGCAG	1 cut(s)	213
Bsh1236I	CGCG	3 cut(s)	89, 135, 563
BshFI	GGCC	1 cut(s)	54
BsiHKAI	GWGCWC	2 cut(s)	372, 460
BsiSI	CCGG	3 cut(s)	72, 269, 359
BslI	CCNNNNNNNGG	1 cut(s)	557
BsmI	GAATGC	1 cut(s)	296
BsnI	GGCC	1 cut(s)	54
Bsp1286I	GDGCHC	2 cut(s)	372, 460
Bsp143I	GATC	3 cut(s)	218, 256, 264
Bsp1720I	GCTNAGC	1 cut(s)	404
Bsp19I	CCATGG	1 cut(s)	22
Bsp68I	TCGCGA	1 cut(s)	89
BspACI	CCGC	5 cut(s)	12, 461, 482, 506, 563
BspANI	GGCC	1 cut(s)	54
BspCNI	CTCAG	2 cut(s)	228, 417
BspFNI	CGCG	3 cut(s)	89, 135, 563
BspLI	GGNNCC	2 cut(s)	76, 304
BspMI	ACCTGC	1 cut(s)	314
BspPI	GGATC	1 cut(s)	259
BssECI	CCNNGG	2 cut(s)	22, 285
BssMI	GATC	3 cut(s)	218, 256, 264
BssT1I	CCWWGG	2 cut(s)	22, 285
BstDEI	CTNAG	2 cut(s)	215, 404
BstDSI	CCRYGG	1 cut(s)	22
BstF5I	GGATG	1 cut(s)	345
BstFNI	CGCG	3 cut(s)	89, 135, 563
BstHHI	GCGC	3 cut(s)	135, 311, 563
BstKTI	GATC	3 cut(s)	221, 259, 267
BstMBI	GATC	3 cut(s)	218, 256, 264
BstMWI	GCNNNNNNNGC	3 cut(s)	60, 74, 132
BstNSI	RCATGY	2 cut(s)	400, 536
BstSCI	CCNGG	1 cut(s)	267
BstSLI	GKGCMC	1 cut(s)	372
BstUI	CGCG	3 cut(s)	89, 135, 563
BstV1I	GCAGC	3 cut(s)	80, 135, 475
BstV2I	GAAGAC	1 cut(s)	179
BsuRI	GGCC	1 cut(s)	54
BtgI	CCRYGG	1 cut(s)	22
BtrI	CACGTC	1 cut(s)	202
BtsCI	GGATG	1 cut(s)	345
BtuMI	TCGCGA	1 cut(s)	89
BveI	ACCTGC	1 cut(s)	314
CfoI	GCGC	3 cut(s)	135, 311, 563
CseI	GACGC	1 cut(s)	173
Csp6I	GTAC	3 cut(s)	94, 280, 579
CspCI	CAANNNNNGTGG	2 cut(s)	101, 136
CviAII	CATG	5 cut(s)	23, 176, 380, 397, 533
CviJI	RGCY	6 cut(s)	54, 71, 77, 126, 403, 408
CviKI_1	RGCY	6 cut(s)	54, 71, 77, 126, 403, 408
CviQI	GTAC	3 cut(s)	94, 280, 579
DdeI	CTNAG	2 cut(s)	215, 404
DpnI	GATC	3 cut(s)	220, 258, 266
DpnII	GATC	3 cut(s)	218, 256, 264
DraI	TTTAAA	1 cut(s)	31
EciI	GGCGGA	1 cut(s)	27
Eco130I	CCWWGG	2 cut(s)	22, 285
EcoT14I	CCWWGG	2 cut(s)	22, 285
ErhI	CCWWGG	2 cut(s)	22, 285
FaeI	CATG	5 cut(s)	26, 179, 383, 400, 536
FaiI	YATR	9 cut(s)	24, 80, 149, 177, 381, 398, 534, 570, 575
FatI	CATG	5 cut(s)	22, 175, 379, 396, 532
Fnu4HI	GCNGC	3 cut(s)	69, 124, 464
FokI	GGATG	1 cut(s)	332
Fsp4HI	GCNGC	3 cut(s)	69, 124, 464
FspBI	CTAG	1 cut(s)	450
GlaI	GCGC	3 cut(s)	134, 310, 562
GluI	GCNGC	3 cut(s)	69, 124, 464
HaeIII	GGCC	1 cut(s)	54
HapII	CCGG	3 cut(s)	72, 269, 359
HgaI	GACGC	1 cut(s)	173
HhaI	GCGC	3 cut(s)	135, 311, 563
Hin1II	CATG	5 cut(s)	26, 179, 383, 400, 536
Hin6I	GCGC	3 cut(s)	133, 309, 561
HinP1I	GCGC	3 cut(s)	133, 309, 561
HincII	GTYRAC	3 cut(s)	109, 205, 241
HindII	GTYRAC	3 cut(s)	109, 205, 241
HinfI	GANTC	1 cut(s)	496
HpaI	GTTAAC	1 cut(s)	241
HpaII	CCGG	3 cut(s)	72, 269, 359
HphI	GGTGA	2 cut(s)	28, 516
Hpy166II	GTNNAC	6 cut(s)	109, 205, 241, 282, 353, 370
Hpy188I	TCNGA	1 cut(s)	218
Hpy188III	TCNNGA	1 cut(s)	88
Hpy8I	GTNNAC	6 cut(s)	109, 205, 241, 282, 353, 370
Hpy99I	CGWCG	4 cut(s)	11, 89, 94, 338
HpyAV	CCTTC	2 cut(s)	293, 531
HpyCH4IV	ACGT	3 cut(s)	92, 201, 468
HpyCH4V	TGCA	6 cut(s)	68, 123, 230, 294, 370, 390
HpyF10VI	GCNNNNNNNGC	3 cut(s)	60, 74, 132
HpyF3I	CTNAG	2 cut(s)	215, 404
HpySE526I	ACGT	3 cut(s)	92, 201, 468
Hsp92II	CATG	5 cut(s)	26, 179, 383, 400, 536
HspAI	GCGC	3 cut(s)	133, 309, 561
KspAI	GTTAAC	1 cut(s)	241
Kzo9I	GATC	3 cut(s)	218, 256, 264
LmnI	GCTCC	1 cut(s)	74
LpnPI	CCDG	5 cut(s)	54, 85, 282, 319, 372
Lsp1109I	GCAGC	3 cut(s)	80, 135, 475
LweI	GCATC	2 cut(s)	205, 537
MaeI	CTAG	1 cut(s)	450
MaeII	ACGT	3 cut(s)	92, 201, 468
MaeIII	GTNAC	1 cut(s)	493
MalI	GATC	3 cut(s)	220, 258, 266
MboI	GATC	3 cut(s)	218, 256, 264
MboII	GAAGA	1 cut(s)	179
MhlI	GDGCHC	2 cut(s)	372, 460
MluCI	AATT	2 cut(s)	326, 553
MlyI	GAGTC	1 cut(s)	490
MnlI	CCTC	7 cut(s)	224, 259, 435, 486, 489, 510, 513
MseI	TTAA	2 cut(s)	30, 240
MslI	CAYNNNNRTG	1 cut(s)	395
MspI	CCGG	3 cut(s)	72, 269, 359
MspR9I	CCNGG	1 cut(s)	269
Mva1269I	GAATGC	1 cut(s)	296
MvnI	CGCG	3 cut(s)	89, 135, 563
MwoI	GCNNNNNNNGC	3 cut(s)	60, 74, 132
NciI	CCSGG	1 cut(s)	269
NcoI	CCATGG	1 cut(s)	22
NdeII	GATC	3 cut(s)	218, 256, 264
NlaIII	CATG	5 cut(s)	26, 179, 383, 400, 536
NlaIV	GGNNCC	2 cut(s)	76, 304
NmuCI	GTSAC	1 cut(s)	493
NruI	TCGCGA	1 cut(s)	89
NspI	RCATGY	2 cut(s)	400, 536
PciI	ACATGT	2 cut(s)	396, 532
PctI	GAATGC	1 cut(s)	296
PkrI	GCNGC	3 cut(s)	70, 125, 465
PleI	GAGTC	1 cut(s)	490
PpsI	GAGTC	1 cut(s)	490
PscI	ACATGT	2 cut(s)	396, 532
PspN4I	GGNNCC	2 cut(s)	76, 304
RruI	TCGCGA	1 cut(s)	89
RsaI	GTAC	3 cut(s)	95, 281, 580
RsaNI	GTAC	3 cut(s)	94, 280, 579
RseI	CAYNNNNRTG	1 cut(s)	395
SaqAI	TTAA	2 cut(s)	30, 240
SatI	GCNGC	3 cut(s)	69, 124, 464
Sau3AI	GATC	3 cut(s)	218, 256, 264
ScaI	AGTACT	1 cut(s)	580
SchI	GAGTC	1 cut(s)	490
ScrFI	CCNGG	1 cut(s)	269
SduI	GDGCHC	2 cut(s)	372, 460
SetI	ASST	6 cut(s)	95, 204, 216, 308, 410, 471
SfaNI	GCATC	2 cut(s)	205, 537
SmiMI	CAYNNNNRTG	1 cut(s)	395
Sse9I	AATT	2 cut(s)	326, 553
SsiI	CCGC	5 cut(s)	12, 461, 482, 506, 563
SspMI	CTAG	1 cut(s)	450
StyD4I	CCNGG	1 cut(s)	267
StyI	CCWWGG	2 cut(s)	22, 285
TaiI	ACGT	3 cut(s)	95, 204, 471
TaqI	TCGA	1 cut(s)	433
TasI	AATT	2 cut(s)	326, 553
TatI	WGTACW	2 cut(s)	279, 578
Tru1I	TTAA	2 cut(s)	30, 240
Tru9I	TTAA	2 cut(s)	30, 240
TseFI	GTSAC	1 cut(s)	493
TseI	GCWGC	3 cut(s)	68, 123, 463
Tsp45I	GTSAC	1 cut(s)	493
TspDTI	ATGAA	4 cut(s)	186, 192, 368, 557
TspGWI	ACGGA	1 cut(s)	338
VneI	GTGCAC	1 cut(s)	368
XapI	RAATTY	2 cut(s)	326, 553
XceI	RCATGY	2 cut(s)	400, 536
XspI	CTAG	1 cut(s)	450
ZrmI	AGTACT	1 cut(s)	580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.