Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016878

Physical Location & Seq

Genomic Coordinates Reverse (-)

9606481 .. 9607038

Gene Structure 558 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1078800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 558 bp

ATGGCCGACGGAGAACCGCACCACGGATACCAACTCACCCCCGAACTCATCGGCCTACTTGGCGTTGCAGCCGGAGCCATAATTGTCACGACGTACCAACTCCTTGTCATCCACTTTTGTTGCCGCCAAATGCGCGAAGAAAACAACATACAACAAGAACAAACACAACATGAACAACGTCGTCAATGGCATGACGTCAACCGAAACTTCAGATCAACAACTGCACGCAGCGTTAATTCGCCCTCCTACGATAAGCTGATCCCGGTTCTGAAGTACACCAAGGAATGCAGTGAAGGAACCTGCGCCGTTTGTTTGTGTGAATTTACCGACGGCGAGGAAATCCGTGTGCTGCCGGAATGTGCACACTTGTTTCATGTGGGGTGCATTGACATGTGGCTCAGCTCACACTCTAACTGCCCACTTTGTCGAACTGATATTATTGCTAAACGAGCACCGCAACACGTTGTTCCTCCTCCGCAAGATGTTGAGGCTCCTCCTCCGCAGAATGCTTTGCCTGCACCACATGTTGTGATACTGATGCCAAGTGTTGGAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

186

Amino Acids

20.7

Weight (kDa)

5.89

Isoelectric Point (pI)

64.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	100 - 143	8.8e-14	Ring finger domain
zf-RING_11	PF17123	101 - 129	1.8e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	101 - 142	3.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	105 - 143	1.2e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016878)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g14040
malus_domestica	MD04G1125800.v1.1 MD12G1078800.v1.1
prunus_persica	Prupe.6G252700_v2.0.a1 Prupe.6G252800_v2.0.a1 Prupe.6G252900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0466921
rosa_laevigata	RLG00000024545
rosa_multiflora	Rmu_sc0017982.1_g000001 Rmu_sc0023775.1_g000001 Rmu_sc0023776.1_g000002
rosa_roxburghii	Rroxscaffold_6G00414060
rosa_rugosa	Rorug03G0084200
rosa_wichuraiana	Rw3G012480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	198
Acc36I	ACCTGC	1 cut(s)	308
AccB7I	CCANNNNNTGG	1 cut(s)	548
AccII	CGCG	1 cut(s)	135
AciI	CCGC	5 cut(s)	17, 124, 455, 476, 500
AclWI	GGATC	1 cut(s)	253
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	320
AcuI	CTGAAG	2 cut(s)	193, 290
AcyI	GRCGYC	1 cut(s)	195
AfaI	GTAC	2 cut(s)	95, 275
AfiI	CCNNNNNNNGG	2 cut(s)	23, 548
AflIII	ACRYGT	3 cut(s)	390, 460, 523
AluBI	AGCT	2 cut(s)	256, 402
AluI	AGCT	2 cut(s)	256, 402
Alw21I	GWGCWC	2 cut(s)	364, 454
Alw44I	GTGCAC	1 cut(s)	360
AlwI	GGATC	1 cut(s)	253
AoxI	GGCC	2 cut(s)	3, 52
ApaLI	GTGCAC	1 cut(s)	360
ApeKI	GCWGC	3 cut(s)	68, 228, 349
ApoI	RAATTY	1 cut(s)	320
AspLEI	GCGC	2 cut(s)	135, 305
AsuC2I	CCSGG	1 cut(s)	263
AsuHPI	GGTGA	1 cut(s)	28
BaeGI	GKGCMC	1 cut(s)	364
Bbv12I	GWGCWC	2 cut(s)	364, 454
BbvI	GCAGC	3 cut(s)	80, 240, 336
BceAI	ACGGC	2 cut(s)	290, 346
BciVI	GTATCC	1 cut(s)	20
BcnI	CCSGG	1 cut(s)	263
BfuAI	ACCTGC	1 cut(s)	308
BfuI	GTATCC	1 cut(s)	20
BglI	GCCNNNNNGGC	1 cut(s)	60
BisI	GCNGC	4 cut(s)	69, 124, 229, 350
BlpI	GCTNAGC	1 cut(s)	398
BlsI	GCNGC	4 cut(s)	70, 125, 230, 351
Bme1390I	CCNGG	1 cut(s)	263
BmiI	GGNNCC	3 cut(s)	76, 298, 492
BmrFI	CCNGG	1 cut(s)	263
BmsI	GCATC	1 cut(s)	528
Bpu1102I	GCTNAGC	1 cut(s)	398
BpuMI	CCSGG	1 cut(s)	263
BsaHI	GRCGYC	1 cut(s)	195
BsaJI	CCNNGG	2 cut(s)	22, 279
Bsc4I	CCNNNNNNNGG	2 cut(s)	23, 548
BseDI	CCNNGG	2 cut(s)	22, 279
BseGI	GGATG	1 cut(s)	108
BseLI	CCNNNNNNNGG	2 cut(s)	23, 548
BseMII	CTCAG	1 cut(s)	412
BseRI	GAGGAG	3 cut(s)	462, 483, 486
BseSI	GKGCMC	1 cut(s)	364
BseXI	GCAGC	3 cut(s)	80, 240, 336
BsgI	GTGCAG	2 cut(s)	207, 501
Bsh1236I	CGCG	1 cut(s)	135
BshFI	GGCC	2 cut(s)	5, 54
BsiHKAI	GWGCWC	2 cut(s)	364, 454
BsiSI	CCGG	3 cut(s)	72, 263, 353
BslI	CCNNNNNNNGG	2 cut(s)	23, 548
BsmI	GAATGC	2 cut(s)	290, 511
BsnI	GGCC	2 cut(s)	5, 54
Bsp1286I	GDGCHC	2 cut(s)	364, 454
Bsp143I	GATC	2 cut(s)	212, 258
Bsp1720I	GCTNAGC	1 cut(s)	398
BspACI	CCGC	5 cut(s)	17, 124, 455, 476, 500
BspANI	GGCC	2 cut(s)	5, 54
BspCNI	CTCAG	1 cut(s)	411
BspFNI	CGCG	1 cut(s)	135
BspLI	GGNNCC	3 cut(s)	76, 298, 492
BspMI	ACCTGC	1 cut(s)	308
BspPI	GGATC	1 cut(s)	253
BssECI	CCNNGG	2 cut(s)	22, 279
BssMI	GATC	2 cut(s)	212, 258
BssNI	GRCGYC	1 cut(s)	195
BssT1I	CCWWGG	1 cut(s)	279
BstACI	GRCGYC	1 cut(s)	195
BstC8I	GCNNGC	2 cut(s)	226, 516
BstDEI	CTNAG	1 cut(s)	398
BstDSI	CCRYGG	1 cut(s)	22
BstF5I	GGATG	1 cut(s)	108
BstFNI	CGCG	1 cut(s)	135
BstHHI	GCGC	2 cut(s)	135, 305
BstKTI	GATC	2 cut(s)	215, 261
BstMBI	GATC	2 cut(s)	212, 258
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 74, 132, 449, 515
BstNSI	RCATGY	2 cut(s)	394, 527
BstSCI	CCNGG	1 cut(s)	261
BstSLI	GKGCMC	1 cut(s)	364
BstUI	CGCG	1 cut(s)	135
BstV1I	GCAGC	3 cut(s)	80, 240, 336
BsuI	GTATCC	1 cut(s)	20
BsuRI	GGCC	2 cut(s)	5, 54
BtgI	CCRYGG	1 cut(s)	22
BtsCI	GGATG	1 cut(s)	108
BtsI	GCAGTG	1 cut(s)	295
BtsIMutI	CAGTG	1 cut(s)	295
BveI	ACCTGC	1 cut(s)	308
Cac8I	GCNNGC	2 cut(s)	226, 516
CfoI	GCGC	2 cut(s)	135, 305
Csp6I	GTAC	2 cut(s)	94, 274
CspCI	CAANNNNNGTGG	2 cut(s)	101, 136
CviAII	CATG	5 cut(s)	170, 191, 374, 391, 524
CviJI	RGCY	8 cut(s)	5, 54, 71, 77, 256, 397, 402, 491
CviKI_1	RGCY	8 cut(s)	5, 54, 71, 77, 256, 397, 402, 491
CviQI	GTAC	2 cut(s)	94, 274
DdeI	CTNAG	1 cut(s)	398
DpnI	GATC	2 cut(s)	214, 260
DpnII	GATC	2 cut(s)	212, 258
EaeI	YGGCCR	1 cut(s)	3
Eco130I	CCWWGG	1 cut(s)	279
Eco57I	CTGAAG	2 cut(s)	193, 290
EcoT14I	CCWWGG	1 cut(s)	279
ErhI	CCWWGG	1 cut(s)	279
FaeI	CATG	5 cut(s)	173, 194, 377, 394, 527
FaiI	YATR	8 cut(s)	80, 149, 171, 192, 375, 392, 525, 556
FatI	CATG	5 cut(s)	169, 190, 373, 390, 523
Fnu4HI	GCNGC	4 cut(s)	69, 124, 229, 350
FokI	GGATG	1 cut(s)	95
Fsp4HI	GCNGC	4 cut(s)	69, 124, 229, 350
GlaI	GCGC	2 cut(s)	134, 304
GluI	GCNGC	4 cut(s)	69, 124, 229, 350
HaeIII	GGCC	2 cut(s)	5, 54
HapII	CCGG	3 cut(s)	72, 263, 353
HhaI	GCGC	2 cut(s)	135, 305
Hin1I	GRCGYC	1 cut(s)	195
Hin1II	CATG	5 cut(s)	173, 194, 377, 394, 527
Hin6I	GCGC	2 cut(s)	133, 303
HinP1I	GCGC	2 cut(s)	133, 303
HincII	GTYRAC	1 cut(s)	199
HindII	GTYRAC	1 cut(s)	199
HpaII	CCGG	3 cut(s)	72, 263, 353
HphI	GGTGA	1 cut(s)	28
Hpy166II	GTNNAC	3 cut(s)	199, 276, 362
Hpy188I	TCNGA	2 cut(s)	212, 270
Hpy188III	TCNNGA	1 cut(s)	88
Hpy8I	GTNNAC	3 cut(s)	199, 276, 362
Hpy99I	CGWCG	4 cut(s)	11, 94, 183, 332
HpyAV	CCTTC	1 cut(s)	287
HpyCH4IV	ACGT	4 cut(s)	92, 178, 195, 462
HpyCH4V	TGCA	6 cut(s)	68, 224, 288, 362, 384, 518
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 74, 132, 449, 515
HpyF3I	CTNAG	1 cut(s)	398
HpySE526I	ACGT	4 cut(s)	92, 178, 195, 462
Hsp92I	GRCGYC	1 cut(s)	195
Hsp92II	CATG	5 cut(s)	173, 194, 377, 394, 527
HspAI	GCGC	2 cut(s)	133, 303
Kzo9I	GATC	2 cut(s)	212, 258
LmnI	GCTCC	2 cut(s)	74, 496
LpnPI	CCDG	5 cut(s)	85, 276, 313, 366, 528
Lsp1109I	GCAGC	3 cut(s)	80, 240, 336
LweI	GCATC	1 cut(s)	528
MaeII	ACGT	4 cut(s)	92, 178, 195, 462
MaeIII	GTNAC	1 cut(s)	85
MalI	GATC	2 cut(s)	214, 260
MboI	GATC	2 cut(s)	212, 258
MboII	GAAGA	1 cut(s)	149
MhlI	GDGCHC	2 cut(s)	364, 454
MluCI	AATT	3 cut(s)	81, 235, 320
MmeI	TCCRAC	1 cut(s)	529
MnlI	CCTC	7 cut(s)	253, 328, 480, 481, 483, 504, 507
MseI	TTAA	1 cut(s)	234
MslI	CAYNNNNRTG	1 cut(s)	389
MspI	CCGG	3 cut(s)	72, 263, 353
MspR9I	CCNGG	1 cut(s)	263
Mva1269I	GAATGC	2 cut(s)	290, 511
MvnI	CGCG	1 cut(s)	135
MwoI	GCNNNNNNNGC	5 cut(s)	60, 74, 132, 449, 515
NciI	CCSGG	1 cut(s)	263
NdeII	GATC	2 cut(s)	212, 258
NlaIII	CATG	5 cut(s)	173, 194, 377, 394, 527
NlaIV	GGNNCC	3 cut(s)	76, 298, 492
NmuCI	GTSAC	1 cut(s)	85
NspI	RCATGY	2 cut(s)	394, 527
PciI	ACATGT	2 cut(s)	390, 523
PctI	GAATGC	2 cut(s)	290, 511
PflMI	CCANNNNNTGG	1 cut(s)	548
PkrI	GCNGC	4 cut(s)	70, 125, 230, 351
PscI	ACATGT	2 cut(s)	390, 523
PspN4I	GGNNCC	3 cut(s)	76, 298, 492
RsaI	GTAC	2 cut(s)	95, 275
RsaNI	GTAC	2 cut(s)	94, 274
RseI	CAYNNNNRTG	1 cut(s)	389
SaqAI	TTAA	1 cut(s)	234
SatI	GCNGC	4 cut(s)	69, 124, 229, 350
Sau3AI	GATC	2 cut(s)	212, 258
ScrFI	CCNGG	1 cut(s)	263
SduI	GDGCHC	2 cut(s)	364, 454
SetI	ASST	7 cut(s)	95, 181, 198, 258, 302, 404, 465
SfaNI	GCATC	1 cut(s)	528
SmiMI	CAYNNNNRTG	1 cut(s)	389
Sse9I	AATT	3 cut(s)	81, 235, 320
SsiI	CCGC	5 cut(s)	17, 124, 455, 476, 500
StyD4I	CCNGG	1 cut(s)	261
StyI	CCWWGG	1 cut(s)	279
TaiI	ACGT	4 cut(s)	95, 181, 198, 465
TaqI	TCGA	1 cut(s)	427
TasI	AATT	3 cut(s)	81, 235, 320
TatI	WGTACW	1 cut(s)	273
TauI	GCSGC	1 cut(s)	126
Tru1I	TTAA	1 cut(s)	234
Tru9I	TTAA	1 cut(s)	234
TscAI	CASTG	1 cut(s)	295
TseFI	GTSAC	1 cut(s)	85
TseI	GCWGC	3 cut(s)	68, 228, 349
Tsp45I	GTSAC	1 cut(s)	85
TspDTI	ATGAA	2 cut(s)	186, 362
TspGWI	ACGGA	3 cut(s)	24, 39, 332
TspRI	CASTG	1 cut(s)	295
Van91I	CCANNNNNTGG	1 cut(s)	548
VneI	GTGCAC	1 cut(s)	360
XapI	RAATTY	1 cut(s)	320
XceI	RCATGY	2 cut(s)	394, 527
ZraI	GACGTC	1 cut(s)	196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.