RchiOBHm_Chr3g0466921

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016878
Physical Location & Seq
Reverse (-)
13217760 .. 13218278
519 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43292

Sequence Viewer

Length: 519 bp
ATGGAAGAACCACACCGGCATTTCCTCTCCAGCCCGTTAATCATTGGCCTTCTCGGCGTCGCAGCCGGAGCCATCATCGTTGCAATATACCACTGCCTAGTCAACAACTGTTGCTTCATCCAAAGCCGCATATCAAGTGCACAACAAAGCGAACAAAGCCAAGTGCTTCCGACTACCAACCACAGCAGGACTAGGAGCATTCCCACAAACACTTTCCCTGAGCATTTGATCCCGACTTACAGATACACCAAGGAATGCAGAGAGGGAATGTGTGCCGTGTGCTTGTGTGACTTCGCCGACGGCGAGGCGATTAGGGTGCTGCCCCAATGCATGCACTTGTTTCATGTGGGCTGCATTGATATGTGGTTGAACTCGCACACAAATTGCCCCTTATGTCGCACTGACATTATCCCTCGGCGCCAACATGTTACAGTGGAGAGGATCGAACCTATAACCTATTCCGATCCTAACTCAATTTCTCAACCTCCGCTAATACCAAATTTGGGTGGGATTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

172

Amino Acids

19.26

Weight (kDa)

6.48

Isoelectric Point (pI)

55.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 86 - 133 4.6e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 90 - 133 7e-13 Ring finger domain
zf-RING_11 PF17123 91 - 119 2e-09 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 417
AciI CCGC 2 cut(s) 127, 488
AclWI GGATC 3 cut(s) 223, 449, 458
AcsI RAATTY 1 cut(s) 499
AcyI GRCGYC 2 cut(s) 57, 418
AfiI CCNNNNNNNGG 1 cut(s) 503
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 1 cut(s) 370
Alw21I GWGCWC 1 cut(s) 142
Alw44I GTGCAC 1 cut(s) 138
AlwI GGATC 3 cut(s) 223, 449, 458
AoxI GGCC 1 cut(s) 46
ApaLI GTGCAC 1 cut(s) 138
ApeKI GCWGC 3 cut(s) 62, 319, 351
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 142
BanI GGYRCC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 3 cut(s) 74, 306, 338
BccI CCATC 1 cut(s) 80
BceAI ACGGC 2 cut(s) 260, 316
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BfaI CTAG 3 cut(s) 98, 192, 517
BfoI RGCGCY 1 cut(s) 421
BglI GCCNNNNNGGC 1 cut(s) 54
BisI GCNGC 4 cut(s) 63, 127, 320, 352
BlsI GCNGC 4 cut(s) 64, 128, 321, 353
BmiI GGNNCC 2 cut(s) 70, 419
BpmI CTGGAG 1 cut(s) 13
Bpu10I CCTNAGC 1 cut(s) 219
BsaHI GRCGYC 2 cut(s) 57, 418
BsaJI CCNNGG 2 cut(s) 249, 413
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse118I RCCGGY 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 249, 413
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMII CTCAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 3 cut(s) 74, 306, 338
BshFI GGCC 1 cut(s) 48
BshNI GGYRCC 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 2 cut(s) 16, 66
BslI CCNNNNNNNGG 1 cut(s) 503
BsmI GAATGC 2 cut(s) 198, 260
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 3 cut(s) 228, 441, 463
BspACI CCGC 2 cut(s) 127, 488
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 70, 419
BspPI GGATC 3 cut(s) 223, 449, 458
BspT107I GGYRCC 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 249, 413
BssMI GATC 3 cut(s) 228, 441, 463
BssNI GRCGYC 2 cut(s) 57, 418
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 110, 433
BstACI GRCGYC 2 cut(s) 57, 418
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 117
BstH2I RGCGCY 1 cut(s) 421
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 3 cut(s) 231, 444, 466
BstMBI GATC 3 cut(s) 228, 441, 463
BstMWI GCNNNNNNNGC 3 cut(s) 54, 68, 156
BstNSI RCATGY 2 cut(s) 334, 428
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 3 cut(s) 74, 306, 338
BsuRI GGCC 1 cut(s) 48
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 3 cut(s) 91, 399, 438
Cac8I GCNNGC 1 cut(s) 332
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 15
CseI GACGC 1 cut(s) 46
CviAII CATG 3 cut(s) 331, 344, 425
CviJI RGCY 7 cut(s) 33, 48, 65, 71, 126, 159, 351
CviKI_1 RGCY 7 cut(s) 33, 48, 65, 71, 126, 159, 351
DdeI CTNAG 1 cut(s) 219
DinI GGCGCC 1 cut(s) 419
DpnI GATC 3 cut(s) 230, 443, 465
DpnII GATC 3 cut(s) 228, 441, 463
Eco130I CCWWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 332
EgeI GGCGCC 1 cut(s) 419
EheI GGCGCC 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 334, 347, 428
FaiI YATR 8 cut(s) 88, 131, 332, 345, 362, 394, 426, 452
FatI CATG 3 cut(s) 330, 343, 424
Fnu4HI GCNGC 4 cut(s) 63, 127, 320, 352
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 4 cut(s) 63, 127, 320, 352
FspBI CTAG 3 cut(s) 98, 192, 517
GlaI GCGC 1 cut(s) 419
GluI GCNGC 4 cut(s) 63, 127, 320, 352
GsuI CTGGAG 1 cut(s) 13
HaeII RGCGCY 1 cut(s) 421
HaeIII GGCC 1 cut(s) 48
HapII CCGG 2 cut(s) 16, 66
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 2 cut(s) 57, 418
Hin1II CATG 3 cut(s) 334, 347, 428
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HpaII CCGG 2 cut(s) 16, 66
Hpy166II GTNNAC 2 cut(s) 103, 140
Hpy188I TCNGA 2 cut(s) 171, 463
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 2 cut(s) 103, 140
Hpy99I CGWCG 2 cut(s) 62, 302
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 110, 433
HpyCH4V TGCA 6 cut(s) 83, 140, 258, 330, 334, 354
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 68, 156
HpyF3I CTNAG 1 cut(s) 219
Hsp92I GRCGYC 2 cut(s) 57, 418
Hsp92II CATG 3 cut(s) 334, 347, 428
HspAI GCGC 1 cut(s) 418
KasI GGCGCC 1 cut(s) 417
Kzo9I GATC 3 cut(s) 228, 441, 463
LmnI GCTCC 2 cut(s) 68, 195
LpnPI CCDG 5 cut(s) 29, 43, 79, 172, 231
Lsp1109I GCAGC 3 cut(s) 74, 306, 338
MaeI CTAG 3 cut(s) 98, 192, 517
MaeIII GTNAC 2 cut(s) 287, 427
MalI GATC 3 cut(s) 230, 443, 465
MboI GATC 3 cut(s) 228, 441, 463
MboII GAAGA 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 3 cut(s) 382, 474, 499
Mly113I GGCGCC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 6 cut(s) 35, 256, 298, 423, 432, 495
Mph1103I ATGCAT 1 cut(s) 332
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 359
MspI CCGG 2 cut(s) 16, 66
Mva1269I GAATGC 2 cut(s) 198, 260
MwoI GCNNNNNNNGC 3 cut(s) 54, 68, 156
NarI GGCGCC 1 cut(s) 418
NdeII GATC 3 cut(s) 228, 441, 463
NlaIII CATG 3 cut(s) 334, 347, 428
NlaIV GGNNCC 2 cut(s) 70, 419
NmeAIII GCCGAG 2 cut(s) 33, 394
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 2 cut(s) 334, 428
PaeI GCATGC 1 cut(s) 334
PciI ACATGT 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 2 cut(s) 198, 260
PkrI GCNGC 4 cut(s) 64, 128, 321, 353
PluTI GGCGCC 1 cut(s) 421
PscI ACATGT 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 70, 419
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 4 cut(s) 63, 127, 320, 352
Sau3AI GATC 3 cut(s) 228, 441, 463
SduI GDGCHC 1 cut(s) 142
SetI ASST 3 cut(s) 451, 458, 487
SfoI GGCGCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 359
SphI GCATGC 1 cut(s) 334
Sse9I AATT 3 cut(s) 382, 474, 499
SsiI CCGC 2 cut(s) 127, 488
SspDI GGCGCC 1 cut(s) 417
SspMI CTAG 3 cut(s) 98, 192, 517
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 110, 433
TaqI TCGA 1 cut(s) 444
TasI AATT 3 cut(s) 382, 474, 499
TauI GCSGC 1 cut(s) 129
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 3 cut(s) 98, 406, 438
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 3 cut(s) 62, 319, 351
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 106, 332
TspRI CASTG 3 cut(s) 98, 406, 438
VneI GTGCAC 1 cut(s) 138
XapI RAATTY 1 cut(s) 499
XceI RCATGY 2 cut(s) 334, 428
XspI CTAG 3 cut(s) 98, 192, 517
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.