MD04G1126500.v1.1

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013835
Physical Location & Seq
Forward (+)
21254031 .. 21255893
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1126500.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGTTTTCTCTTCCATTCCAGCTTATCTTGATCCAGCCAACTGGCAGCAGCAACCAAATCAGCACCACCACTTAACAGGAAGTAACACCAACAACAACATCGACAACCACAACTCTCACTTCCTTCCACCTCCTCCGCAGCCTCCGCCTTCAAACCCTCATGGAGGCAGTGGGGTAGGTGGCTCAGGCTCGATCAGGCCAGGCTCCATGGCAGATCGAGCCCGCATGGCCAACATCCCCCTCCCAGAGCAGGCTCTAAAATGCCCGCGGTGCGAGTCTACCAACACCAAGTTCTGCTACTTCAACAACTACAGCCTCACGCAGCCCCGCCACTTTTGCAAGACCTGCAGAAGGTACTGGACACGCGGTGGGGCCCTGCGTAACGTTCCAGTTGGCGGTGGCTGCAGGAGAAACAAAAGGAGCAAAGGAACCAGCAGCAGCGCCTCCAAGTCCCCAGCCAGCAGCTCGGACCAGCCAACCGGTTCGGCCAGTTCGTCAGGCCACGCAAATTTAGGGTTAAACAACAACAGTGCGGCCGGCTCGGCGGATAATGATAACATGATGATGAGCCCGCAAATCCCACCGCTCCGTTTTATGTCGTCTTTCCCCACCGACATGGCCTTAAATTTCGGCATGATGAACAATAATAATAGTAGTAATTATGGAATTGGAGACTTGAGTGGTTTTCATCAGATGGGGAGTGGTCTAGGAGCTAGCGGTGGATCGCTCTTTAATTTCCAGCAGCAGACGCCGTCGTTTCCTTTCATGGCGGGGTTGGATCCTACGTCAGCGGGGCTGATGTTCGAGAACAGTGGAGTTGAGCTAACGGGGTATAATCAGGTGCGGCCAAGAGCACCGTCTGCGGGGATGAGTATACCTCAAATGGCTTCGGTGAAGACGGAATTAGAAAGTCAGCAACAACAACAACAACAGAATCAGGAGTTGAATTTGTCGAGGCAGTTGTTGGGGATGAATAATAATTCAGGAAATGATCAGTTCTGGGGCAATGGCGGCCGCGGCGGTGGTGGTGCTAGTACTACTACTGCATGGACTGATCTTTCGGGGTTTAACTCTACTTCCACTACTAGCAATAATATTAACAATCCCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

371

Amino Acids

39.42

Weight (kDa)

9.13

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 84 - 140 9.8e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013835)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02460
fragaria_vesca FvH4_6g14000
malus_domestica MD04G1126500.v1.1 MD12G1078400.v1.1
prunus_persica Prupe.6G253300_v2.0.a1
pyrus_communis pycom04g11350 pycom12g13510
rosa_chinensis RchiOBHm_Chr3g0466831
rosa_laevigata RLG00000024548
rosa_multiflora Rmu_sc0017982.1_g000002
rosa_roxburghii Rroxscaffold_6G00414100
rosa_rugosa Rorug03G0083900
rosa_samantha Rh3AG131600 Rh3BG152700 Rh3CG152900 Rh3DG153800
rosa_wichuraiana Rw3G012440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AccBSI CCGCTC 1 cut(s) 586
AccI GTMKAC 2 cut(s) 278, 874
AccII CGCG 3 cut(s) 268, 366, 1017
AclI AACGTT 1 cut(s) 384
AclWI GGATC 4 cut(s) 26, 730, 773, 786
AcoI YGGCCR 5 cut(s) 228, 486, 534, 845, 1012
AcsI RAATTY 3 cut(s) 508, 625, 946
AcyI GRCGYC 1 cut(s) 749
AdeI CACNNNGTG 1 cut(s) 368
AfaI GTAC 2 cut(s) 356, 1036
AfiI CCNNNNNNNGG 5 cut(s) 164, 250, 351, 395, 863
AgeI ACCGGT 1 cut(s) 479
AgsI TTSAA 3 cut(s) 153, 304, 946
AjnI CCWGG 1 cut(s) 199
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 4 cut(s) 23, 465, 713, 823
AluI AGCT 4 cut(s) 23, 465, 713, 823
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 1 cut(s) 666
AlwI GGATC 4 cut(s) 26, 730, 773, 786
AoxI GGCC 9 cut(s) 197, 228, 372, 486, 499, 534, 618, 845, 1012
ApaI GGGCCC 1 cut(s) 376
ApeKI GCWGC 9 cut(s) 46, 49, 139, 322, 402, 435, 438, 462, 742
ApoI RAATTY 3 cut(s) 508, 625, 946
ArsI GACNNNNNNTTYG 2 cut(s) 1042, 1074
AsiGI ACCGGT 1 cut(s) 479
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 3 cut(s) 372, 373, 469
AsuHPI GGTGA 1 cut(s) 904
AsuNHI GCTAGC 1 cut(s) 713
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 1 cut(s) 778
BanII GRGCYC 3 cut(s) 223, 376, 572
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 9 cut(s) 58, 61, 151, 334, 389, 447, 450, 474, 754
BccI CCATC 1 cut(s) 688
BceAI ACGGC 1 cut(s) 736
BciT130I CCWGG 1 cut(s) 201
BclI TGATCA 1 cut(s) 991
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 4 cut(s) 707, 714, 1032, 1086
BfmI CTRYAG 3 cut(s) 310, 346, 403
BfoI RGCGCY 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 353
BglI GCCNNNNNGGC 2 cut(s) 227, 542
BmcAI AGTACT 1 cut(s) 1036
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 372, 373, 469
BmiI GGNNCC 5 cut(s) 205, 373, 374, 430, 780
BmrFI CCNGG 1 cut(s) 201
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 902
BplI GAGNNNNNCTC 2 cut(s) 862, 894
Bpu10I CCTNAGC 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 749
BsaJI CCNNGG 3 cut(s) 207, 266, 1015
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 250, 351, 395, 863
Bse118I RCCGGY 2 cut(s) 479, 536
Bse1I ACTGG 4 cut(s) 47, 362, 389, 489
Bse3DI GCAATG 1 cut(s) 1012
BseBI CCWGG 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 207, 266, 1015
BseGI GGATG 3 cut(s) 234, 873, 975
BseLI CCNNNNNNNGG 5 cut(s) 164, 250, 351, 395, 863
BseMI GCAATG 1 cut(s) 1012
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 4 cut(s) 47, 362, 389, 489
BseRI GAGGAG 1 cut(s) 123
BseSI GKGCMC 1 cut(s) 376
BseX3I CGGCCG 2 cut(s) 534, 1012
BseXI GCAGC 9 cut(s) 58, 61, 151, 334, 389, 447, 450, 474, 754
BseYI CCCAGC 1 cut(s) 454
Bsh1236I CGCG 3 cut(s) 268, 366, 1017
Bsh1285I CGRYCG 2 cut(s) 537, 1015
BshFI GGCC 9 cut(s) 199, 230, 374, 488, 501, 536, 620, 847, 1014
BshTI ACCGGT 1 cut(s) 479
BsiEI CGRYCG 2 cut(s) 537, 1015
BsiHKAI GWGCWC 1 cut(s) 856
BsiSI CCGG 2 cut(s) 480, 537
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 5 cut(s) 164, 250, 351, 395, 863
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 9 cut(s) 199, 230, 374, 488, 501, 536, 620, 847, 1014
Bsp120I GGGCCC 1 cut(s) 372
Bsp1286I GDGCHC 4 cut(s) 223, 376, 572, 856
Bsp143I GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
Bsp19I CCATGG 1 cut(s) 207
BspANI GGCC 9 cut(s) 199, 230, 374, 488, 501, 536, 620, 847, 1014
BspCNI CTCAG 1 cut(s) 197
BspFNI CGCG 3 cut(s) 268, 366, 1017
BspLI GGNNCC 5 cut(s) 205, 373, 374, 430, 780
BspMAI CTGCAG 2 cut(s) 350, 407
BspMI ACCTGC 1 cut(s) 353
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 4 cut(s) 26, 730, 773, 786
BsrBI CCGCTC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 1012
BsrFI RCCGGY 2 cut(s) 479, 536
BsrI ACTGG 4 cut(s) 47, 362, 389, 489
BssAI RCCGGY 2 cut(s) 479, 536
BssECI CCNNGG 3 cut(s) 207, 266, 1015
BssMI GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
BssNAI GTATAC 1 cut(s) 875
BssNI GRCGYC 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 207
Bst1107I GTATAC 1 cut(s) 875
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 3 cut(s) 530, 812, 858
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 749
BstAPI GCANNNNNTGC 2 cut(s) 345, 860
BstC8I GCNNGC 7 cut(s) 223, 252, 266, 460, 538, 572, 715
BstDEI CTNAG 1 cut(s) 184
BstDSI CCRYGG 3 cut(s) 207, 266, 1015
BstENI CCTNNNNNAGG 2 cut(s) 162, 349
BstF5I GGATG 3 cut(s) 234, 873, 975
BstFNI CGCG 3 cut(s) 268, 366, 1017
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 7 cut(s) 34, 195, 217, 725, 781, 994, 1057
BstMAI GTCTC 1 cut(s) 666
BstMBI GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
BstMCI CGRYCG 2 cut(s) 537, 1015
BstNI CCWGG 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 3 cut(s) 310, 346, 403
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 3 cut(s) 268, 366, 1017
BstV1I GCAGC 9 cut(s) 58, 61, 151, 334, 389, 447, 450, 474, 754
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 1 cut(s) 778
BstXI CCANNNNNNTGG 2 cut(s) 42, 616
BstYI RGATCY 1 cut(s) 778
BstZ17I GTATAC 1 cut(s) 875
BstZI CGGCCG 2 cut(s) 534, 1012
BsuRI GGCC 9 cut(s) 199, 230, 374, 488, 501, 536, 620, 847, 1014
BtgI CCRYGG 3 cut(s) 207, 266, 1015
BtsCI GGATG 3 cut(s) 234, 873, 975
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 3 cut(s) 175, 535, 817
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 7 cut(s) 223, 252, 266, 460, 538, 572, 715
CciNI GCGGCCGC 1 cut(s) 1012
CfoI GCGC 1 cut(s) 443
Cfr10I RCCGGY 2 cut(s) 479, 536
Cfr13I GGNCC 3 cut(s) 372, 373, 469
Cfr42I CCGCGG 2 cut(s) 269, 1018
CseI GACGC 1 cut(s) 757
Csp6I GTAC 2 cut(s) 355, 1035
CspAI ACCGGT 1 cut(s) 479
CviAII CATG 8 cut(s) 161, 208, 226, 559, 616, 634, 766, 1047
CviQI GTAC 2 cut(s) 355, 1035
DdeI CTNAG 1 cut(s) 184
DpnI GATC 7 cut(s) 33, 194, 216, 724, 780, 993, 1056
DpnII GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
DraIII CACNNNGTG 1 cut(s) 368
EaeI YGGCCR 5 cut(s) 228, 486, 534, 845, 1012
EagI CGGCCG 2 cut(s) 534, 1012
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 2 cut(s) 135, 560
EclXI CGGCCG 2 cut(s) 534, 1012
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 3 cut(s) 223, 376, 572
Eco47I GGWCC 1 cut(s) 469
Eco52I CGGCCG 2 cut(s) 534, 1012
EcoNI CCTNNNNNAGG 2 cut(s) 162, 349
EcoO109I RGGNCCY 2 cut(s) 372, 373
EcoRII CCWGG 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 3 cut(s) 223, 376, 572
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 8 cut(s) 164, 211, 229, 562, 619, 637, 769, 1050
FaqI GGGAC 1 cut(s) 436
FatI CATG 8 cut(s) 160, 207, 225, 558, 615, 633, 765, 1046
FauI CCCGC 7 cut(s) 230, 273, 335, 579, 763, 784, 856
FbaI TGATCA 1 cut(s) 991
FblI GTMKAC 2 cut(s) 278, 874
FokI GGATG 3 cut(s) 221, 880, 982
FriOI GRGCYC 3 cut(s) 223, 376, 572
FspBI CTAG 4 cut(s) 707, 714, 1032, 1086
GlaI GCGC 1 cut(s) 442
GsaI CCCAGC 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 9 cut(s) 199, 230, 374, 488, 501, 536, 620, 847, 1014
HapII CCGG 2 cut(s) 480, 537
HgaI GACGC 1 cut(s) 757
HhaI GCGC 1 cut(s) 443
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 8 cut(s) 164, 211, 229, 562, 619, 637, 769, 1050
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HinfI GANTC 2 cut(s) 275, 934
HpaII CCGG 2 cut(s) 480, 537
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 2 cut(s) 279, 875
Hpy188I TCNGA 2 cut(s) 469, 693
Hpy188III TCNNGA 4 cut(s) 29, 805, 938, 984
Hpy8I GTNNAC 2 cut(s) 279, 875
Hpy99I CGWCG 1 cut(s) 757
HpyAV CCTTC 3 cut(s) 134, 159, 345
HpyCH4III ACNGT 3 cut(s) 530, 812, 858
HpyCH4IV ACGT 2 cut(s) 384, 785
HpyCH4V TGCA 4 cut(s) 339, 348, 405, 1046
HpyF3I CTNAG 1 cut(s) 184
HpySE526I ACGT 2 cut(s) 384, 785
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 8 cut(s) 164, 211, 229, 562, 619, 637, 769, 1050
HspAI GCGC 1 cut(s) 441
KroI GCCGGC 1 cut(s) 536
KroNI GCCGGC 1 cut(s) 538
Ksp22I TGATCA 1 cut(s) 991
KspI CCGCGG 2 cut(s) 269, 1018
Kzo9I GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
LmnI GCTCC 4 cut(s) 209, 420, 591, 710
Lsp1109I GCAGC 9 cut(s) 58, 61, 151, 334, 389, 447, 450, 474, 754
MaeI CTAG 4 cut(s) 707, 714, 1032, 1086
MaeII ACGT 2 cut(s) 384, 785
MaeIII GTNAC 2 cut(s) 83, 380
MalI GATC 7 cut(s) 33, 194, 216, 724, 780, 993, 1056
MbiI CCGCTC 1 cut(s) 586
MboI GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
MboII GAAGA 2 cut(s) 3, 907
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 4 cut(s) 223, 376, 572, 856
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 8 cut(s) 508, 625, 658, 666, 733, 902, 946, 979
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 756
Mox20I TGGCCA 1 cut(s) 230
MroNI GCCGGC 1 cut(s) 536
MscI TGGCCA 1 cut(s) 230
MseI TTAA 6 cut(s) 74, 518, 623, 732, 1068, 1098
MslI CAYNNNNRTG 2 cut(s) 563, 614
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 3 cut(s) 268, 791, 1017
MspI CCGG 2 cut(s) 480, 537
MspR9I CCNGG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 201
MvnI CGCG 3 cut(s) 268, 366, 1017
NaeI GCCGGC 1 cut(s) 538
NcoI CCATGG 1 cut(s) 207
NdeII GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
NgoMIV GCCGGC 1 cut(s) 536
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 8 cut(s) 164, 211, 229, 562, 619, 637, 769, 1050
NlaIV GGNNCC 5 cut(s) 205, 373, 374, 430, 780
NmeAIII GCCGAG 1 cut(s) 521
NotI GCGGCCGC 1 cut(s) 1012
PcsI WCGNNNNNNNCGW 1 cut(s) 491
PdiI GCCGGC 1 cut(s) 538
PfeI GAWTC 1 cut(s) 934
PflFI GACNNNGTC 1 cut(s) 751
PinAI ACCGGT 1 cut(s) 479
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 5 cut(s) 205, 373, 374, 430, 780
PspOMI GGGCCC 1 cut(s) 372
PspPI GGNCC 3 cut(s) 372, 373, 469
PstI CTGCAG 2 cut(s) 350, 407
PsuI RGATCY 1 cut(s) 778
PsyI GACNNNGTC 1 cut(s) 751
RsaI GTAC 2 cut(s) 356, 1036
RsaNI GTAC 2 cut(s) 355, 1035
RseI CAYNNNNRTG 2 cut(s) 563, 614
SacII CCGCGG 2 cut(s) 269, 1018
SaqAI TTAA 6 cut(s) 74, 518, 623, 732, 1068, 1098
Sau3AI GATC 7 cut(s) 31, 192, 214, 722, 778, 991, 1054
Sau96I GGNCC 3 cut(s) 372, 373, 469
ScaI AGTACT 1 cut(s) 1036
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 4 cut(s) 223, 376, 572, 856
SfcI CTRYAG 3 cut(s) 310, 346, 403
Sfr303I CCGCGG 2 cut(s) 269, 1018
SgrBI CCGCGG 2 cut(s) 269, 1018
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 563, 614
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
Sse9I AATT 8 cut(s) 508, 625, 658, 666, 733, 902, 946, 979
SspI AATATT 1 cut(s) 1096
SspMI CTAG 4 cut(s) 707, 714, 1032, 1086
StyD4I CCNGG 1 cut(s) 199
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 530, 812, 858
TaiI ACGT 2 cut(s) 387, 788
TaqI TCGA 5 cut(s) 102, 191, 217, 804, 953
TasI AATT 8 cut(s) 508, 625, 658, 666, 733, 902, 946, 979
TatI WGTACW 1 cut(s) 1034
TauI GCSGC 5 cut(s) 536, 847, 1014, 1017, 1020
TfiI GAWTC 1 cut(s) 934
Tru1I TTAA 6 cut(s) 74, 518, 623, 732, 1068, 1098
Tru9I TTAA 6 cut(s) 74, 518, 623, 732, 1068, 1098
TscAI CASTG 3 cut(s) 175, 535, 817
TseI GCWGC 9 cut(s) 46, 49, 139, 322, 402, 435, 438, 462, 742
TspDTI ATGAA 4 cut(s) 653, 677, 754, 986
TspGWI ACGGA 2 cut(s) 578, 914
TspRI CASTG 3 cut(s) 175, 535, 817
Tth111I GACNNNGTC 1 cut(s) 751
VpaK11BI GGWCC 1 cut(s) 469
XagI CCTNNNNNAGG 2 cut(s) 162, 349
XapI RAATTY 3 cut(s) 508, 625, 946
XmiI GTMKAC 2 cut(s) 278, 874
XspI CTAG 4 cut(s) 707, 714, 1032, 1086
ZrmI AGTACT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.