pycom04g11350

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013835
Physical Location & Seq
Forward (+)
14237302 .. 14238606
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11350.1

Sequence Viewer

Length: 1110 bp
ATGGTTTTCTCTTCCATTCCAGCTTATCTTGATCCAGCCAACTGGCAGCAGCAACCAAATCAGCACCACCACTTAACAGGAAGCAACACCAACAACATTAGCAACAACAACATCGACAACAACAACTCTCACTTCCTTCCACCTCCTCCGCAGCCGCCGCCTTCAAACCCTCATGGAGGCAGTGGGGTAGGTGGCTCAGGCTCGATCAGGCCAGGCTCCATGGCAGATCGAGCCCGCATGGCCAATATCCCCCTCCCAGAGCAGGCACTAAAATGCCCGCGGTGCGAGTCCACCAACACCAAGTTCTGCTACTTCAACAACTACAGCCTCACGCAGCCCCGCCACTTTTGCAAGACCTGCAGAAGGTACTGGACACGCGGTGGGGCCCTGCGTAACGTTCCAGTTGGCGGTGGCTGCAGGAGAAACAAAAGGAGCAAGGGAACCAGCAGCAGCGCCTCCAAGTCCCCAGCCAGCAGCTCGGACCCGCCAACCGGCTCGGCCAGTTCGTCAGGCCACGCAAATTTAGGGTTAAACAACAACAGTACAGCCGGCTCGGCGGATAATGATAACATGATGATGAGCCCGCAAATCCCACCGCTCCGTTTTATGTCGCCTTTCGCCACCGACATGGCTTTAAATTTCGGCATGATGAACAATAATTATGGAATTGGAGACTTGAATGGTTTTCATCAGATGGGGAGTGGTCCAGGAGCTAGCGGTGGATCGATCTTTAATTTCCAGCAGCAGACGCCGTCGTTTCCTTTCATGGCGGGGTTGGATCCTACGTCAGCGGGGATGATGTTCGAGAGCAGCGGAGTTGAGCTAACGGGGTATAATCAGGTGCGGCCAAGAGCACCGTCTGCGGGGATGAGTATACCTCAAATGGCTTCGGTGAAGACGGAATTAGAAAGTCAGCAACAACAACAACAGAATCAGGAGTTGAATTTGTCGAGGCAGTTGTTGGGGATGAATAATAATTCAGGAAATGATCAGTTCTGGAGCAATGGCGGCCGCGGCGGTGGTGGTGCTAGTACTACTACTGCATGGACTGATCTTTCGGGGTTTAATTCTACTTCCACTACTAGCAATAATATTAACAATCCCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

370

Amino Acids

39.31

Weight (kDa)

9.13

Isoelectric Point (pI)

52.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 88 - 144 9.8e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013835)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02460
fragaria_vesca FvH4_6g14000
malus_domestica MD04G1126500.v1.1 MD12G1078400.v1.1
prunus_persica Prupe.6G253300_v2.0.a1
pyrus_communis pycom04g11350 pycom12g13510
rosa_chinensis RchiOBHm_Chr3g0466831
rosa_laevigata RLG00000024548
rosa_multiflora Rmu_sc0017982.1_g000002
rosa_roxburghii Rroxscaffold_6G00414100
rosa_rugosa Rorug03G0083900
rosa_samantha Rh3AG131600 Rh3BG152700 Rh3CG152900 Rh3DG153800
rosa_wichuraiana Rw3G012440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 365
AccBSI CCGCTC 1 cut(s) 598
AccI GTMKAC 1 cut(s) 874
AccII CGCG 3 cut(s) 280, 378, 1014
AclI AACGTT 1 cut(s) 396
AclWI GGATC 4 cut(s) 26, 730, 773, 786
AcoI YGGCCR 4 cut(s) 240, 498, 845, 1009
AcsI RAATTY 3 cut(s) 520, 637, 943
AcyI GRCGYC 1 cut(s) 749
AdeI CACNNNGTG 1 cut(s) 380
AfaI GTAC 3 cut(s) 368, 544, 1033
AfiI CCNNNNNNNGG 6 cut(s) 176, 262, 363, 407, 491, 863
AgsI TTSAA 4 cut(s) 165, 316, 679, 943
AjnI CCWGG 2 cut(s) 211, 706
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AluBI AGCT 4 cut(s) 23, 477, 713, 823
AluI AGCT 4 cut(s) 23, 477, 713, 823
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 1 cut(s) 666
AlwI GGATC 4 cut(s) 26, 730, 773, 786
AoxI GGCC 7 cut(s) 209, 240, 384, 498, 511, 845, 1009
ApaI GGGCCC 1 cut(s) 388
ApoI RAATTY 3 cut(s) 520, 637, 943
ArsI GACNNNNNNTTYG 2 cut(s) 1039, 1071
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 4 cut(s) 384, 385, 481, 704
AsuHPI GGTGA 1 cut(s) 904
AsuNHI GCTAGC 1 cut(s) 713
AvaII GGWCC 2 cut(s) 481, 704
BaeGI GKGCMC 1 cut(s) 388
BalI TGGCCA 1 cut(s) 242
BamHI GGATCC 1 cut(s) 778
BanII GRGCYC 3 cut(s) 235, 388, 584
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 856
BccI CCATC 1 cut(s) 688
BceAI ACGGC 1 cut(s) 736
BciT130I CCWGG 2 cut(s) 213, 708
BclI TGATCA 1 cut(s) 988
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 3 cut(s) 714, 1029, 1083
BfmI CTRYAG 3 cut(s) 322, 358, 415
BfoI RGCGCY 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 365
BglI GCCNNNNNGGC 2 cut(s) 239, 554
BmcAI AGTACT 1 cut(s) 1033
Bme1390I CCNGG 2 cut(s) 213, 708
Bme18I GGWCC 2 cut(s) 481, 704
BmgT120I GGNCC 4 cut(s) 384, 385, 481, 704
BmiI GGNNCC 6 cut(s) 217, 385, 386, 442, 483, 780
BmrFI CCNGG 2 cut(s) 213, 708
BmtI GCTAGC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 902
BplI GAGNNNNNCTC 2 cut(s) 862, 894
BpmI CTGGAG 1 cut(s) 1018
Bpu10I CCTNAGC 1 cut(s) 196
Bsa29I ATCGAT 1 cut(s) 725
BsaHI GRCGYC 1 cut(s) 749
BsaJI CCNNGG 3 cut(s) 219, 278, 1012
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 262, 363, 407, 491, 863
Bse118I RCCGGY 2 cut(s) 491, 548
Bse1I ACTGG 4 cut(s) 47, 374, 401, 501
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 2 cut(s) 213, 708
BseCI ATCGAT 1 cut(s) 725
BseDI CCNNGG 3 cut(s) 219, 278, 1012
BseGI GGATG 3 cut(s) 801, 873, 972
BseLI CCNNNNNNNGG 6 cut(s) 176, 262, 363, 407, 491, 863
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 4 cut(s) 47, 374, 401, 501
BseRI GAGGAG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 388
BseX3I CGGCCG 1 cut(s) 1009
BseYI CCCAGC 1 cut(s) 466
Bsh1236I CGCG 3 cut(s) 280, 378, 1014
Bsh1285I CGRYCG 1 cut(s) 1012
BshFI GGCC 7 cut(s) 211, 242, 386, 500, 513, 847, 1011
BshVI ATCGAT 1 cut(s) 725
BsiEI CGRYCG 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 856
BsiSI CCGG 2 cut(s) 492, 549
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 6 cut(s) 176, 262, 363, 407, 491, 863
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 7 cut(s) 211, 242, 386, 500, 513, 847, 1011
Bsp120I GGGCCC 1 cut(s) 384
Bsp1286I GDGCHC 4 cut(s) 235, 388, 584, 856
Bsp143I GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
Bsp19I CCATGG 1 cut(s) 219
BspANI GGCC 7 cut(s) 211, 242, 386, 500, 513, 847, 1011
BspCNI CTCAG 1 cut(s) 209
BspDI ATCGAT 1 cut(s) 725
BspFNI CGCG 3 cut(s) 280, 378, 1014
BspLI GGNNCC 6 cut(s) 217, 385, 386, 442, 483, 780
BspMAI CTGCAG 2 cut(s) 362, 419
BspMI ACCTGC 1 cut(s) 365
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 4 cut(s) 26, 730, 773, 786
BsrBI CCGCTC 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 2 cut(s) 491, 548
BsrI ACTGG 4 cut(s) 47, 374, 401, 501
BssAI RCCGGY 2 cut(s) 491, 548
BssECI CCNNGG 3 cut(s) 219, 278, 1012
BssMI GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
BssNAI GTATAC 1 cut(s) 875
BssNI GRCGYC 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 875
Bst2UI CCWGG 2 cut(s) 213, 708
Bst4CI ACNGT 2 cut(s) 542, 858
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 749
BstAPI GCANNNNNTGC 2 cut(s) 357, 860
BstC8I GCNNGC 7 cut(s) 235, 264, 278, 472, 550, 584, 715
BstDEI CTNAG 1 cut(s) 196
BstDSI CCRYGG 3 cut(s) 219, 278, 1012
BstENI CCTNNNNNAGG 2 cut(s) 174, 361
BstF5I GGATG 3 cut(s) 801, 873, 972
BstFNI CGCG 3 cut(s) 280, 378, 1014
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 8 cut(s) 34, 207, 229, 725, 729, 781, 991, 1054
BstMAI GTCTC 1 cut(s) 666
BstMBI GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
BstMCI CGRYCG 1 cut(s) 1012
BstNI CCWGG 2 cut(s) 213, 708
BstSCI CCNGG 2 cut(s) 211, 706
BstSFI CTRYAG 3 cut(s) 322, 358, 415
BstSLI GKGCMC 1 cut(s) 388
BstUI CGCG 3 cut(s) 280, 378, 1014
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 1 cut(s) 778
BstXI CCANNNNNNTGG 2 cut(s) 42, 628
BstYI RGATCY 1 cut(s) 778
BstZ17I GTATAC 1 cut(s) 875
BstZI CGGCCG 1 cut(s) 1009
Bsu15I ATCGAT 1 cut(s) 725
BsuRI GGCC 7 cut(s) 211, 242, 386, 500, 513, 847, 1011
BsuTUI ATCGAT 1 cut(s) 725
BtgI CCRYGG 3 cut(s) 219, 278, 1012
BtsCI GGATG 3 cut(s) 801, 873, 972
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 7 cut(s) 235, 264, 278, 472, 550, 584, 715
CciNI GCGGCCGC 1 cut(s) 1009
CfoI GCGC 1 cut(s) 455
Cfr10I RCCGGY 2 cut(s) 491, 548
Cfr13I GGNCC 4 cut(s) 384, 385, 481, 704
Cfr42I CCGCGG 2 cut(s) 281, 1015
ClaI ATCGAT 1 cut(s) 725
CseI GACGC 1 cut(s) 757
Csp6I GTAC 3 cut(s) 367, 543, 1032
CviAII CATG 8 cut(s) 173, 220, 238, 571, 628, 646, 766, 1044
CviQI GTAC 3 cut(s) 367, 543, 1032
DdeI CTNAG 1 cut(s) 196
DpnI GATC 8 cut(s) 33, 206, 228, 724, 728, 780, 990, 1053
DpnII GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
DraI TTTAAA 1 cut(s) 636
DraIII CACNNNGTG 1 cut(s) 380
EaeI YGGCCR 4 cut(s) 240, 498, 845, 1009
EagI CGGCCG 1 cut(s) 1009
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 572
EclXI CGGCCG 1 cut(s) 1009
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 3 cut(s) 235, 388, 584
Eco47I GGWCC 2 cut(s) 481, 704
Eco52I CGGCCG 1 cut(s) 1009
EcoNI CCTNNNNNAGG 2 cut(s) 174, 361
EcoO109I RGGNCCY 2 cut(s) 384, 385
EcoRII CCWGG 2 cut(s) 211, 706
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 3 cut(s) 235, 388, 584
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 8 cut(s) 176, 223, 241, 574, 631, 649, 769, 1047
FaqI GGGAC 1 cut(s) 448
FatI CATG 8 cut(s) 172, 219, 237, 570, 627, 645, 765, 1043
FauI CCCGC 8 cut(s) 242, 285, 347, 492, 591, 763, 784, 856
FbaI TGATCA 1 cut(s) 988
FblI GTMKAC 1 cut(s) 874
FokI GGATG 3 cut(s) 808, 880, 979
FriOI GRGCYC 3 cut(s) 235, 388, 584
FspBI CTAG 3 cut(s) 714, 1029, 1083
GlaI GCGC 1 cut(s) 454
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 1018
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 7 cut(s) 211, 242, 386, 500, 513, 847, 1011
HapII CCGG 2 cut(s) 492, 549
HgaI GACGC 1 cut(s) 757
HhaI GCGC 1 cut(s) 455
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 8 cut(s) 176, 223, 241, 574, 631, 649, 769, 1047
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HinfI GANTC 2 cut(s) 287, 931
HpaII CCGG 2 cut(s) 492, 549
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 2 cut(s) 291, 875
Hpy188I TCNGA 2 cut(s) 481, 693
Hpy188III TCNNGA 5 cut(s) 29, 805, 935, 981, 997
Hpy8I GTNNAC 2 cut(s) 291, 875
Hpy99I CGWCG 1 cut(s) 757
HpyAV CCTTC 3 cut(s) 146, 171, 357
HpyCH4III ACNGT 2 cut(s) 542, 858
HpyCH4IV ACGT 2 cut(s) 396, 785
HpyCH4V TGCA 4 cut(s) 351, 360, 417, 1043
HpyF3I CTNAG 1 cut(s) 196
HpySE526I ACGT 2 cut(s) 396, 785
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 8 cut(s) 176, 223, 241, 574, 631, 649, 769, 1047
HspAI GCGC 1 cut(s) 453
KroI GCCGGC 1 cut(s) 548
KroNI GCCGGC 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 988
KspI CCGCGG 2 cut(s) 281, 1015
Kzo9I GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
LmnI GCTCC 5 cut(s) 221, 432, 603, 710, 999
MaeI CTAG 3 cut(s) 714, 1029, 1083
MaeII ACGT 2 cut(s) 396, 785
MaeIII GTNAC 1 cut(s) 392
MalI GATC 8 cut(s) 33, 206, 228, 724, 728, 780, 990, 1053
MbiI CCGCTC 1 cut(s) 598
MboI GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
MboII GAAGA 2 cut(s) 3, 907
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 4 cut(s) 235, 388, 584, 856
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 9 cut(s) 520, 637, 658, 666, 733, 902, 943, 976, 1066
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 756
MnlI CCTC 9 cut(s) 153, 156, 170, 180, 263, 338, 466, 888, 945
Mox20I TGGCCA 1 cut(s) 242
MroNI GCCGGC 1 cut(s) 548
MscI TGGCCA 1 cut(s) 242
MseI TTAA 6 cut(s) 74, 530, 635, 732, 1065, 1095
MslI CAYNNNNRTG 3 cut(s) 271, 575, 626
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 4 cut(s) 280, 791, 813, 1014
MspI CCGG 2 cut(s) 492, 549
MspR9I CCNGG 2 cut(s) 213, 708
MvaI CCWGG 2 cut(s) 213, 708
MvnI CGCG 3 cut(s) 280, 378, 1014
NaeI GCCGGC 1 cut(s) 550
NcoI CCATGG 1 cut(s) 219
NdeII GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
NgoMIV GCCGGC 1 cut(s) 548
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 8 cut(s) 176, 223, 241, 574, 631, 649, 769, 1047
NlaIV GGNNCC 6 cut(s) 217, 385, 386, 442, 483, 780
NmeAIII GCCGAG 2 cut(s) 476, 533
NotI GCGGCCGC 1 cut(s) 1009
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 550
PfeI GAWTC 1 cut(s) 931
PflFI GACNNNGTC 1 cut(s) 751
PfoI TCCNGGA 1 cut(s) 706
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp1406I AACGTT 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 211, 706
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 2 cut(s) 211, 706
PspN4I GGNNCC 6 cut(s) 217, 385, 386, 442, 483, 780
PspOMI GGGCCC 1 cut(s) 384
PspPI GGNCC 4 cut(s) 384, 385, 481, 704
PstI CTGCAG 2 cut(s) 362, 419
PsuI RGATCY 1 cut(s) 778
PsyI GACNNNGTC 1 cut(s) 751
RsaI GTAC 3 cut(s) 368, 544, 1033
RsaNI GTAC 3 cut(s) 367, 543, 1032
RseI CAYNNNNRTG 3 cut(s) 271, 575, 626
SacII CCGCGG 2 cut(s) 281, 1015
SaqAI TTAA 6 cut(s) 74, 530, 635, 732, 1065, 1095
Sau3AI GATC 8 cut(s) 31, 204, 226, 722, 726, 778, 988, 1051
Sau96I GGNCC 4 cut(s) 384, 385, 481, 704
ScaI AGTACT 1 cut(s) 1033
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 213, 708
SduI GDGCHC 4 cut(s) 235, 388, 584, 856
SfcI CTRYAG 3 cut(s) 322, 358, 415
Sfr303I CCGCGG 2 cut(s) 281, 1015
SgrBI CCGCGG 2 cut(s) 281, 1015
SinI GGWCC 2 cut(s) 481, 704
SmiMI CAYNNNNRTG 3 cut(s) 271, 575, 626
Sse9I AATT 9 cut(s) 520, 637, 658, 666, 733, 902, 943, 976, 1066
SspI AATATT 1 cut(s) 1093
SspMI CTAG 3 cut(s) 714, 1029, 1083
StyD4I CCNGG 2 cut(s) 211, 706
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 542, 858
TaiI ACGT 2 cut(s) 399, 788
TaqI TCGA 6 cut(s) 114, 203, 229, 725, 804, 950
TasI AATT 9 cut(s) 520, 637, 658, 666, 733, 902, 943, 976, 1066
TatI WGTACW 2 cut(s) 542, 1031
TauI GCSGC 6 cut(s) 157, 160, 847, 1011, 1014, 1017
TfiI GAWTC 1 cut(s) 931
Tru1I TTAA 6 cut(s) 74, 530, 635, 732, 1065, 1095
Tru9I TTAA 6 cut(s) 74, 530, 635, 732, 1065, 1095
TscAI CASTG 1 cut(s) 187
TspDTI ATGAA 4 cut(s) 665, 677, 754, 983
TspGWI ACGGA 2 cut(s) 590, 914
TspRI CASTG 1 cut(s) 187
Tth111I GACNNNGTC 1 cut(s) 751
VpaK11BI GGWCC 2 cut(s) 481, 704
XagI CCTNNNNNAGG 2 cut(s) 174, 361
XapI RAATTY 3 cut(s) 520, 637, 943
XmiI GTMKAC 1 cut(s) 874
XspI CTAG 3 cut(s) 714, 1029, 1083
ZrmI AGTACT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.