MD12G1078400.v1.1

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013835
Physical Location & Seq
Reverse (-)
9561904 .. 9563780
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1078400.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGGTTTTCTCTTCCATTCCAGCTTATCTTGATCCGGCCAACTGGCAGCAGCAACCAAATCATCACCACCACTTAACAGAAAGCAACACCAATAACATTAGCAACAACAACATCAACAACAACTCTCACTTCCTTCCGCCTCCTCCTCCGCCGCTGCCTTCAAACACTCATGACGGCTGTGGGGTAGGTGGTGCGGGCTCGATCAGGCCAGGCTCTATGGCAGATCGAGCTCGCATGGCCAACATCCCCCTCCCTGAGCAAGCACTAAAATGTCCGCGGTGCGAATCCACCAACACTAAGTTCTGCTACTTCAACAACTACAGCCTCACACAGCCCCGCCACTTCTGCAAGACTTGTAGAAGGTACTGGACGAGAGGCGGGGCCCTGCGAAATGTTCCAGTTGGCGGCGGCTGCAGGAGAAACAAAAGGAGCAAAGGAACCAGTAGCAGCGCCTCCAAGTCCCCAGCCAGCAGCTCGGACAGGCCAACCGGTTCGGCCGGTTCGTCGGGCCACGCAAATTTAGGGTTAAACAACAGTAGCACGGCCGGCTCGGCGGATAATAATAACATGAGGATGAGCCCGCACATCCCACCGCTGCGTTTCATGTCTCCTTTCTCCACCGACATGGCCTTAAATTTCGGCATGATGAACAATAATAATAGCAGTAATTATGGAGTTGGAGACTTGAATGGTTTTCATCAGATGGGGAGTGGTCTAGGAGCTAGCGGTGGATCGCTCTTTACTTTCCAGCAGCAGACGCCGTCATTTCCGTTCATGGCCGGGTTGGATCCTACGCCGGCGGGGCTTATGTTCGAGAGCAGCGGAGTTGAGGTACCGGGGTATAATCAGGTGCGGCCGAGAGTACCGTTTGCTGGGATGAGTATATCTCAAATGGCTTCGGTGAAGATGGAATTAGAAAGTCAGCAGCAACAACAACAGAATCAGGAGTTGAATTTGTCGAGGCAGTTGTTGGGGATGAATAATAATTCAGGAAATGATCAGTTCTGGAGTAATGGCAGCGGCCGCGGCAGTGGTGGTTCTAGTACTACTACTGCATGGACTGATCATTTGGGGTTTAACCCTACTTCCACTACTAGCAATAATATTAACAATCCCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

374

Amino Acids

40.04

Weight (kDa)

9.32

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 87 - 143 1e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013835)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02460
fragaria_vesca FvH4_6g14000
malus_domestica MD04G1126500.v1.1 MD12G1078400.v1.1
prunus_persica Prupe.6G253300_v2.0.a1
pyrus_communis pycom04g11350 pycom12g13510
rosa_chinensis RchiOBHm_Chr3g0466831
rosa_laevigata RLG00000024548
rosa_multiflora Rmu_sc0017982.1_g000002
rosa_roxburghii Rroxscaffold_6G00414100
rosa_rugosa Rorug03G0083900
rosa_samantha Rh3AG131600 Rh3BG152700 Rh3CG152900 Rh3DG153800
rosa_wichuraiana Rw3G012440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 832
AccB1I GGYRCC 1 cut(s) 832
AccII CGCG 2 cut(s) 277, 1026
AclWI GGATC 4 cut(s) 26, 739, 782, 795
AcoI YGGCCR 7 cut(s) 36, 237, 495, 543, 777, 854, 1021
AcsI RAATTY 3 cut(s) 517, 634, 952
AcyI GRCGYC 1 cut(s) 758
AfaI GTAC 4 cut(s) 365, 834, 864, 1045
AfiI CCNNNNNNNGG 2 cut(s) 404, 872
AgeI ACCGGT 1 cut(s) 488
AgsI TTSAA 4 cut(s) 162, 313, 688, 952
AjnI CCWGG 1 cut(s) 208
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 4 cut(s) 23, 230, 474, 722
AluI AGCT 4 cut(s) 23, 230, 474, 722
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 2 cut(s) 612, 675
AlwI GGATC 4 cut(s) 26, 739, 782, 795
ApaI GGGCCC 1 cut(s) 385
ApoI RAATTY 3 cut(s) 517, 634, 952
ArsI GACNNNNNNTTYG 2 cut(s) 1051, 1083
AsiGI ACCGGT 1 cut(s) 488
Asp718I GGTACC 1 cut(s) 832
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 3 cut(s) 381, 382, 508
AsuC2I CCSGG 2 cut(s) 781, 837
AsuHPI GGTGA 2 cut(s) 56, 913
AsuNHI GCTAGC 1 cut(s) 722
BaeGI GKGCMC 1 cut(s) 385
BaeI ACNNNNGTAYC 2 cut(s) 824, 857
BalI TGGCCA 1 cut(s) 239
BamHI GGATCC 1 cut(s) 787
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 4 cut(s) 200, 232, 385, 581
Bbv12I GWGCWC 1 cut(s) 232
BccI CCATC 2 cut(s) 697, 901
BceAI ACGGC 3 cut(s) 190, 558, 745
BciT130I CCWGG 1 cut(s) 210
BclI TGATCA 2 cut(s) 997, 1063
BcnI CCSGG 2 cut(s) 781, 837
BcoDI GTCTC 2 cut(s) 612, 675
BfaI CTAG 4 cut(s) 716, 723, 1041, 1095
BfmI CTRYAG 2 cut(s) 319, 412
BfoI RGCGCY 1 cut(s) 453
BglI GCCNNNNNGGC 2 cut(s) 551, 802
BmcAI AGTACT 1 cut(s) 1045
Bme1390I CCNGG 3 cut(s) 210, 781, 837
BmgT120I GGNCC 3 cut(s) 381, 382, 508
BmiI GGNNCC 5 cut(s) 382, 383, 439, 789, 834
BmrFI CCNGG 3 cut(s) 210, 781, 837
BmtI GCTAGC 1 cut(s) 726
BplI GAGNNNNNCTC 2 cut(s) 871, 903
BpmI CTGGAG 1 cut(s) 1027
Bpu10I CCTNAGC 1 cut(s) 255
BpuMI CCSGG 2 cut(s) 781, 837
BsaHI GRCGYC 1 cut(s) 758
BsaJI CCNNGG 3 cut(s) 275, 836, 1024
BsaWI WCCGGW 1 cut(s) 488
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 872
Bse118I RCCGGY 4 cut(s) 488, 497, 545, 796
Bse1I ACTGG 4 cut(s) 47, 371, 398, 441
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 275, 836, 1024
BseGI GGATG 5 cut(s) 243, 579, 585, 882, 981
BseLI CCNNNNNNNGG 2 cut(s) 404, 872
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 4 cut(s) 47, 371, 398, 441
BseRI GAGGAG 2 cut(s) 132, 135
BseSI GKGCMC 1 cut(s) 385
BseX3I CGGCCG 4 cut(s) 495, 543, 854, 1021
BseYI CCCAGC 2 cut(s) 463, 872
Bsh1236I CGCG 2 cut(s) 277, 1026
Bsh1285I CGRYCG 4 cut(s) 498, 546, 857, 1024
BshNI GGYRCC 1 cut(s) 832
BshTI ACCGGT 1 cut(s) 488
BsiEI CGRYCG 4 cut(s) 498, 546, 857, 1024
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 7 cut(s) 35, 489, 498, 546, 780, 797, 836
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 2 cut(s) 404, 872
BsmAI GTCTC 2 cut(s) 612, 675
BsmFI GGGAC 1 cut(s) 445
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 4 cut(s) 200, 232, 385, 581
Bsp143I GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 2 cut(s) 277, 1026
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 5 cut(s) 382, 383, 439, 789, 834
BspMAI CTGCAG 1 cut(s) 416
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 4 cut(s) 26, 739, 782, 795
BspT107I GGYRCC 1 cut(s) 832
BsrFI RCCGGY 4 cut(s) 488, 497, 545, 796
BsrI ACTGG 4 cut(s) 47, 371, 398, 441
BssAI RCCGGY 4 cut(s) 488, 497, 545, 796
BssECI CCNNGG 3 cut(s) 275, 836, 1024
BssMI GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
BssNI GRCGYC 1 cut(s) 758
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 536, 867
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 758
BstC8I GCNNGC 8 cut(s) 196, 232, 261, 469, 547, 581, 724, 798
BstDEI CTNAG 2 cut(s) 255, 297
BstDSI CCRYGG 2 cut(s) 275, 1024
BstF5I GGATG 5 cut(s) 243, 579, 585, 882, 981
BstFNI CGCG 2 cut(s) 277, 1026
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 7 cut(s) 34, 204, 226, 734, 790, 1000, 1066
BstMAI GTCTC 2 cut(s) 612, 675
BstMBI GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
BstMCI CGRYCG 4 cut(s) 498, 546, 857, 1024
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 3 cut(s) 208, 779, 835
BstSFI CTRYAG 2 cut(s) 319, 412
BstSLI GKGCMC 1 cut(s) 385
BstUI CGCG 2 cut(s) 277, 1026
BstX2I RGATCY 1 cut(s) 787
BstXI CCANNNNNNTGG 1 cut(s) 625
BstYI RGATCY 1 cut(s) 787
BstZI CGGCCG 4 cut(s) 495, 543, 854, 1021
BtgI CCRYGG 2 cut(s) 275, 1024
BtsCI GGATG 5 cut(s) 243, 579, 585, 882, 981
BtsI GCAGTG 1 cut(s) 1036
BtsIMutI CAGTG 1 cut(s) 1036
Cac8I GCNNGC 8 cut(s) 196, 232, 261, 469, 547, 581, 724, 798
CciI TCATGA 1 cut(s) 169
CciNI GCGGCCGC 1 cut(s) 1021
CfoI GCGC 1 cut(s) 452
Cfr10I RCCGGY 4 cut(s) 488, 497, 545, 796
Cfr13I GGNCC 3 cut(s) 381, 382, 508
Cfr42I CCGCGG 2 cut(s) 278, 1027
CseI GACGC 1 cut(s) 766
Csp6I GTAC 4 cut(s) 364, 833, 863, 1044
CspAI ACCGGT 1 cut(s) 488
CviAII CATG 8 cut(s) 170, 235, 568, 604, 625, 643, 775, 1056
CviQI GTAC 4 cut(s) 364, 833, 863, 1044
DdeI CTNAG 2 cut(s) 255, 297
DpnI GATC 7 cut(s) 33, 203, 225, 733, 789, 999, 1065
DpnII GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
EaeI YGGCCR 7 cut(s) 36, 237, 495, 543, 777, 854, 1021
EagI CGGCCG 4 cut(s) 495, 543, 854, 1021
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 3 cut(s) 126, 138, 569
Ecl136II GAGCTC 1 cut(s) 230
EclXI CGGCCG 4 cut(s) 495, 543, 854, 1021
Eco24I GRGCYC 4 cut(s) 200, 232, 385, 581
Eco52I CGGCCG 4 cut(s) 495, 543, 854, 1021
Eco53kI GAGCTC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 230
EcoO109I RGGNCCY 2 cut(s) 381, 382
EcoRII CCWGG 1 cut(s) 208
EcoT38I GRGCYC 4 cut(s) 200, 232, 385, 581
FaeI CATG 8 cut(s) 173, 238, 571, 607, 628, 646, 778, 1059
FaqI GGGAC 1 cut(s) 445
FatI CATG 8 cut(s) 169, 234, 567, 603, 624, 642, 774, 1055
FauI CCCGC 5 cut(s) 187, 344, 371, 588, 793
FbaI TGATCA 2 cut(s) 997, 1063
FokI GGATG 5 cut(s) 230, 572, 586, 889, 988
FriOI GRGCYC 4 cut(s) 200, 232, 385, 581
FspBI CTAG 4 cut(s) 716, 723, 1041, 1095
GlaI GCGC 1 cut(s) 451
GsaI CCCAGC 2 cut(s) 467, 876
GsuI CTGGAG 1 cut(s) 1027
HaeII RGCGCY 1 cut(s) 453
HapII CCGG 7 cut(s) 35, 489, 498, 546, 780, 797, 836
HgaI GACGC 1 cut(s) 766
HhaI GCGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 758
Hin1II CATG 8 cut(s) 173, 238, 571, 607, 628, 646, 778, 1059
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 2 cut(s) 284, 940
HpaII CCGG 7 cut(s) 35, 489, 498, 546, 780, 797, 836
HphI GGTGA 2 cut(s) 56, 913
Hpy188I TCNGA 2 cut(s) 478, 702
Hpy188III TCNNGA 6 cut(s) 29, 170, 814, 944, 990, 1006
Hpy99I CGWCG 1 cut(s) 508
HpyAV CCTTC 3 cut(s) 143, 168, 354
HpyCH4III ACNGT 2 cut(s) 536, 867
HpyCH4V TGCA 3 cut(s) 348, 414, 1055
HpyF3I CTNAG 2 cut(s) 255, 297
Hsp92I GRCGYC 1 cut(s) 758
Hsp92II CATG 8 cut(s) 173, 238, 571, 607, 628, 646, 778, 1059
HspAI GCGC 1 cut(s) 450
KpnI GGTACC 1 cut(s) 836
KroI GCCGGC 2 cut(s) 545, 796
KroNI GCCGGC 2 cut(s) 547, 798
Ksp22I TGATCA 2 cut(s) 997, 1063
KspI CCGCGG 2 cut(s) 278, 1027
Kzo9I GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
LmnI GCTCC 2 cut(s) 429, 719
MaeI CTAG 4 cut(s) 716, 723, 1041, 1095
MalI GATC 7 cut(s) 33, 203, 225, 733, 789, 999, 1065
MboI GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
MboII GAAGA 2 cut(s) 3, 916
MflI RGATCY 1 cut(s) 787
MhlI GDGCHC 4 cut(s) 200, 232, 385, 581
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 6 cut(s) 517, 634, 667, 911, 952, 985
MluNI TGGCCA 1 cut(s) 239
MmeI TCCRAC 2 cut(s) 658, 765
Mox20I TGGCCA 1 cut(s) 239
MreI CGCCGGCG 1 cut(s) 796
MroNI GCCGGC 2 cut(s) 545, 796
MscI TGGCCA 1 cut(s) 239
MseI TTAA 5 cut(s) 74, 527, 632, 1077, 1107
MslI CAYNNNNRTG 3 cut(s) 268, 572, 623
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 6 cut(s) 154, 277, 595, 822, 1020, 1026
MspI CCGG 7 cut(s) 35, 489, 498, 546, 780, 797, 836
MspR9I CCNGG 3 cut(s) 210, 781, 837
MvaI CCWGG 1 cut(s) 210
MvnI CGCG 2 cut(s) 277, 1026
NaeI GCCGGC 2 cut(s) 547, 798
NciI CCSGG 2 cut(s) 781, 837
NdeII GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
NgoMIV GCCGGC 2 cut(s) 545, 796
NheI GCTAGC 1 cut(s) 722
NlaIII CATG 8 cut(s) 173, 238, 571, 607, 628, 646, 778, 1059
NlaIV GGNNCC 5 cut(s) 382, 383, 439, 789, 834
NmeAIII GCCGAG 2 cut(s) 530, 882
NotI GCGGCCGC 1 cut(s) 1021
PagI TCATGA 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PdiI GCCGGC 2 cut(s) 547, 798
PfeI GAWTC 2 cut(s) 284, 940
PflFI GACNNNGTC 1 cut(s) 760
PinAI ACCGGT 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 208
PspFI CCCAGC 2 cut(s) 463, 872
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 5 cut(s) 382, 383, 439, 789, 834
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 3 cut(s) 381, 382, 508
PstI CTGCAG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 787
PsyI GACNNNGTC 1 cut(s) 760
RsaI GTAC 4 cut(s) 365, 834, 864, 1045
RsaNI GTAC 4 cut(s) 364, 833, 863, 1044
RseI CAYNNNNRTG 3 cut(s) 268, 572, 623
SacI GAGCTC 1 cut(s) 232
SacII CCGCGG 2 cut(s) 278, 1027
SaqAI TTAA 5 cut(s) 74, 527, 632, 1077, 1107
Sau3AI GATC 7 cut(s) 31, 201, 223, 731, 787, 997, 1063
Sau96I GGNCC 3 cut(s) 381, 382, 508
ScaI AGTACT 1 cut(s) 1045
ScrFI CCNGG 3 cut(s) 210, 781, 837
SduI GDGCHC 4 cut(s) 200, 232, 385, 581
SetI ASST 8 cut(s) 25, 190, 232, 365, 476, 724, 834, 852
SfcI CTRYAG 2 cut(s) 319, 412
Sfr303I CCGCGG 2 cut(s) 278, 1027
SgrAI CRCCGGYG 1 cut(s) 796
SgrBI CCGCGG 2 cut(s) 278, 1027
SmiMI CAYNNNNRTG 3 cut(s) 268, 572, 623
Sse9I AATT 6 cut(s) 517, 634, 667, 911, 952, 985
SspI AATATT 1 cut(s) 1105
SspMI CTAG 4 cut(s) 716, 723, 1041, 1095
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 3 cut(s) 208, 779, 835
TaaI ACNGT 2 cut(s) 536, 867
TaqI TCGA 4 cut(s) 200, 226, 813, 959
TasI AATT 6 cut(s) 517, 634, 667, 911, 952, 985
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 7 cut(s) 154, 408, 411, 856, 1023, 1026, 1029
TfiI GAWTC 2 cut(s) 284, 940
Tru1I TTAA 5 cut(s) 74, 527, 632, 1077, 1107
Tru9I TTAA 5 cut(s) 74, 527, 632, 1077, 1107
TscAI CASTG 1 cut(s) 1036
TspDTI ATGAA 5 cut(s) 592, 662, 686, 763, 992
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 1 cut(s) 1036
Tth111I GACNNNGTC 1 cut(s) 760
XapI RAATTY 3 cut(s) 517, 634, 952
XspI CTAG 4 cut(s) 716, 723, 1041, 1095
ZrmI AGTACT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.