MD04G1130500.v1.1

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012954
Physical Location & Seq
Reverse (-)
21705185 .. 21708429
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1130500.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCAACAGTGGCAGTGGCCCCACGTGTCCCATTGGTTATCTCAAATAATAGCATCCGTCAAAGTCATAAATGTAGTTGGGTAGGAGCTCCACAAAATTCGACCCTTTCTTTTCAATCCACTTCATTTTCTGCTGCATCCTTGTTTATCCAGAGAAGGGCGAGAAGGGGTTTTGGGAGAGCAGGAGTTGTCACAAAGGCGATGGCGGATTCGGGTGCAAGCACAGTCCTTGTCACTGGAGCTGGTGGTAGAACTGGTAAACTGGTTTATGAGAAGTTAAAGGAGAGGTCAGACCAGTATGTGGCGAGGGGTCTGGTTAGGAGTGAAGAGAGCAAACAGAGCATTGGTGGAGCAGATGATCTTTATATTGGAGATATAAGGGATGCTGACAGCATTCTACCTGCAATCCAAGGGATCGATGCTCTCGTTATCCTTACAAGTGCTGTGCCTAAGATGAAACCTGGATTCGATCCAACTAAAGGTGGGAGGCCTGAGTTCTATTTTGAAGACGGGTCATATCCTGAACAGGTTGATTGGATTGGACAGAAAAACCAAATAGATGCTGCTAAGGCTGCGGGAGTGAAGCAGATTGTGTTGGTTGGGTCTATGGGTGGAACGGATCTTAACAATCCCCTGAACAAATTGGGTGATGGCAATATATTGGTTTGGAAGAGGAAGGCTGAGCAGTATTTGGCTGACTCCGGAATTCCATACACAATCATAAGGGCCGGAGGCTTGCAAGACAAAGAAGGCGGCATCCGTGAACTACTTATAGGGAAGGATGACGAGCTTCTTAAGACCGAAACAAGAACCATTGCTAGACCTGATGTTGCAGAAGTCTGCATTCAGGCACTGCAGTTCGAGGAGGCCAAGTTTAAGGCATTTGATTTGGCCTCAAAGCCGGAGGGAACTGGGACACCGACCAAGGATTTTAAGGCTTTGTTTTCCCAGATCACAACCCGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

323

Amino Acids

34.7

Weight (kDa)

9.04

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 74 - 241 1.9e-07 NmrA-like family
NAD_binding_10 PF13460 80 - 286 7.3e-33 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 301
AccIII TCCGGA 1 cut(s) 701
AciI CCGC 3 cut(s) 206, 575, 753
AclWI GGATC 3 cut(s) 422, 464, 627
AcsI RAATTY 2 cut(s) 97, 705
AcvI CACGTG 1 cut(s) 26
AfiI CCNNNNNNNGG 3 cut(s) 157, 301, 479
AflII CTTAAG 1 cut(s) 794
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 2 cut(s) 116, 506
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 3 cut(s) 89, 242, 790
AluI AGCT 3 cut(s) 89, 242, 790
Alw21I GWGCWC 1 cut(s) 91
AlwI GGATC 3 cut(s) 422, 464, 627
AlwNI CAGNNNCTG 1 cut(s) 853
Aor13HI TCCGGA 1 cut(s) 701
AoxI GGCC 5 cut(s) 18, 488, 726, 867, 891
ApeKI GCWGC 3 cut(s) 134, 563, 572
ApoI RAATTY 2 cut(s) 97, 705
AspS9I GGNCC 2 cut(s) 19, 726
AsuHPI GGTGA 1 cut(s) 659
BanII GRGCYC 1 cut(s) 91
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 91
BbvI GCAGC 3 cut(s) 121, 550, 559
BccI CCATC 2 cut(s) 196, 644
BciT130I CCWGG 1 cut(s) 462
BfaI CTAG 1 cut(s) 819
BfmI CTRYAG 1 cut(s) 854
BfrI CTTAAG 1 cut(s) 794
BfuAI ACCTGC 1 cut(s) 409
BisI GCNGC 4 cut(s) 135, 564, 573, 754
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 4 cut(s) 136, 565, 574, 755
Bme1390I CCNGG 1 cut(s) 462
BmgT120I GGNCC 2 cut(s) 19, 726
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 1 cut(s) 462
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 6 cut(s) 63, 146, 373, 409, 550, 765
BmuI ACTGGG 1 cut(s) 921
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 681
Bsa29I ATCGAT 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 409, 924
BsaWI WCCGGW 1 cut(s) 701
BsaXI ACNNNNNCTCC 2 cut(s) 857, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 301, 479
Bse1I ACTGG 5 cut(s) 241, 259, 267, 295, 916
Bse3DI GCAATG 1 cut(s) 813
BseAI TCCGGA 1 cut(s) 701
BseBI CCWGG 1 cut(s) 462
BseCI ATCGAT 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 409, 924
BseGI GGATG 5 cut(s) 54, 137, 388, 756, 787
BseLI CCNNNNNNNGG 3 cut(s) 157, 301, 479
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 2 cut(s) 483, 672
BseNI ACTGG 5 cut(s) 241, 259, 267, 295, 916
BseRI GAGGAG 1 cut(s) 878
BseXI GCAGC 3 cut(s) 121, 550, 559
BshFI GGCC 5 cut(s) 20, 490, 728, 869, 893
BshVI ATCGAT 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 3 cut(s) 702, 729, 902
BslFI GGGAC 2 cut(s) 14, 928
BslI CCNNNNNNNGG 3 cut(s) 157, 301, 479
BsmFI GGGAC 2 cut(s) 14, 928
BsmI GAATGC 2 cut(s) 393, 843
BsnI GGCC 5 cut(s) 20, 490, 728, 869, 893
Bsp1286I GDGCHC 1 cut(s) 91
Bsp13I TCCGGA 1 cut(s) 701
Bsp143I GATC 5 cut(s) 358, 414, 469, 619, 951
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 3 cut(s) 206, 575, 753
BspANI GGCC 5 cut(s) 20, 490, 728, 869, 893
BspCNI CTCAG 2 cut(s) 484, 673
BspDI ATCGAT 1 cut(s) 417
BspEI TCCGGA 1 cut(s) 701
BspLI GGNNCC 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 3 cut(s) 422, 464, 627
BspTI CTTAAG 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 813
BsrI ACTGG 5 cut(s) 241, 259, 267, 295, 916
BssECI CCNNGG 2 cut(s) 409, 924
BssMI GATC 5 cut(s) 358, 414, 469, 619, 951
BssT1I CCWWGG 2 cut(s) 409, 924
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 10, 226
Bst6I CTCTTC 2 cut(s) 321, 665
BstAFI CTTAAG 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 220, 737
BstDEI CTNAG 4 cut(s) 450, 492, 567, 681
BstF5I GGATG 5 cut(s) 54, 137, 388, 756, 787
BstKTI GATC 5 cut(s) 361, 417, 472, 622, 954
BstMBI GATC 5 cut(s) 358, 414, 469, 619, 951
BstMWI GCNNNNNNNGC 4 cut(s) 11, 339, 569, 572
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 1 cut(s) 854
BstV1I GCAGC 3 cut(s) 121, 550, 559
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu15I ATCGAT 1 cut(s) 417
BsuRI GGCC 5 cut(s) 20, 490, 728, 869, 893
BsuTUI ATCGAT 1 cut(s) 417
BtgZI GCGATG 1 cut(s) 215
BtsCI GGATG 5 cut(s) 54, 137, 388, 756, 787
BtsI GCAGTG 2 cut(s) 21, 851
BtsIMutI CAGTG 4 cut(s) 15, 21, 234, 851
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 220, 737
CaiI CAGNNNCTG 1 cut(s) 853
Cfr13I GGNCC 2 cut(s) 19, 726
ClaI ATCGAT 1 cut(s) 417
DdeI CTNAG 4 cut(s) 450, 492, 567, 681
DpnI GATC 5 cut(s) 360, 416, 471, 621, 953
DpnII GATC 5 cut(s) 358, 414, 469, 619, 951
Eam1104I CTCTTC 2 cut(s) 321, 665
EarI CTCTTC 2 cut(s) 321, 665
EciI GGCGGA 1 cut(s) 221
Ecl136II GAGCTC 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 409, 924
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 89
Eco72I CACGTG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 460
EcoT14I CCWWGG 2 cut(s) 409, 924
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 2 cut(s) 409, 924
FaqI GGGAC 2 cut(s) 14, 928
FauI CCCGC 1 cut(s) 568
Fnu4HI GCNGC 4 cut(s) 135, 564, 573, 754
FokI GGATG 5 cut(s) 41, 124, 395, 743, 794
FriOI GRGCYC 1 cut(s) 91
Fsp4HI GCNGC 4 cut(s) 135, 564, 573, 754
FspBI CTAG 1 cut(s) 819
GluI GCNGC 4 cut(s) 135, 564, 573, 754
GsuI CTGGAG 1 cut(s) 258
HaeIII GGCC 5 cut(s) 20, 490, 728, 869, 893
HapII CCGG 3 cut(s) 702, 729, 902
HinfI GANTC 3 cut(s) 209, 465, 698
HpaII CCGG 3 cut(s) 702, 729, 902
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 2 cut(s) 260, 764
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 3 cut(s) 151, 521, 702
Hpy8I GTNNAC 2 cut(s) 260, 764
HpyAV CCTTC 5 cut(s) 150, 159, 670, 743, 772
HpyCH4III ACNGT 2 cut(s) 10, 226
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 7 cut(s) 137, 218, 404, 739, 833, 843, 856
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 339, 569, 572
HpyF3I CTNAG 4 cut(s) 450, 492, 567, 681
HpySE526I ACGT 1 cut(s) 25
Kpn2I TCCGGA 1 cut(s) 701
Kzo9I GATC 5 cut(s) 358, 414, 469, 619, 951
LmnI GCTCC 4 cut(s) 86, 94, 239, 350
Lsp1109I GCAGC 3 cut(s) 121, 550, 559
LweI GCATC 6 cut(s) 63, 146, 373, 409, 550, 765
MaeI CTAG 1 cut(s) 819
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 2 cut(s) 190, 232
MalI GATC 5 cut(s) 360, 416, 471, 621, 953
MboI GATC 5 cut(s) 358, 414, 469, 619, 951
MboII GAAGA 3 cut(s) 338, 518, 682
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 3 cut(s) 97, 641, 705
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 497
MnlI CCTC 9 cut(s) 279, 300, 480, 666, 725, 856, 859, 898, 904
MroI TCCGGA 1 cut(s) 701
MseI TTAA 5 cut(s) 278, 624, 795, 876, 933
MspCI CTTAAG 1 cut(s) 794
MspI CCGG 3 cut(s) 702, 729, 902
MspR9I CCNGG 1 cut(s) 462
Mva1269I GAATGC 2 cut(s) 393, 843
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 4 cut(s) 11, 339, 569, 572
NdeII GATC 5 cut(s) 358, 414, 469, 619, 951
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 190, 232
PceI AGGCCT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PctI GAATGC 2 cut(s) 393, 843
PfeI GAWTC 2 cut(s) 209, 465
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 4 cut(s) 136, 565, 574, 755
PleI GAGTC 1 cut(s) 692
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 1 cut(s) 692
Ppu21I YACGTR 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 460
PspCI CACGTG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 2 cut(s) 19, 726
PstI CTGCAG 1 cut(s) 858
PstNI CAGNNNCTG 1 cut(s) 853
PsuI RGATCY 1 cut(s) 619
SacI GAGCTC 1 cut(s) 91
SaqAI TTAA 5 cut(s) 278, 624, 795, 876, 933
SatI GCNGC 4 cut(s) 135, 564, 573, 754
Sau3AI GATC 5 cut(s) 358, 414, 469, 619, 951
Sau96I GGNCC 2 cut(s) 19, 726
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 91
SfaNI GCATC 6 cut(s) 63, 146, 373, 409, 550, 765
SfcI CTRYAG 1 cut(s) 854
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
Sse9I AATT 3 cut(s) 97, 641, 705
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 3 cut(s) 206, 575, 753
SspMI CTAG 1 cut(s) 819
SstI GAGCTC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 2 cut(s) 409, 924
TaaI ACNGT 2 cut(s) 10, 226
TaiI ACGT 1 cut(s) 28
TaqI TCGA 4 cut(s) 101, 417, 468, 861
TaqII GACCGA 1 cut(s) 815
TasI AATT 3 cut(s) 97, 641, 705
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 2 cut(s) 209, 465
Tru1I TTAA 5 cut(s) 278, 624, 795, 876, 933
Tru9I TTAA 5 cut(s) 278, 624, 795, 876, 933
TscAI CASTG 4 cut(s) 15, 21, 241, 858
TseFI GTSAC 2 cut(s) 190, 232
TseI GCWGC 3 cut(s) 134, 563, 572
Tsp45I GTSAC 2 cut(s) 190, 232
TspDTI ATGAA 2 cut(s) 114, 470
TspGWI ACGGA 3 cut(s) 47, 632, 749
TspRI CASTG 4 cut(s) 15, 21, 241, 858
Van91I CCANNNNNTGG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 794
XapI RAATTY 2 cut(s) 97, 705
XspI CTAG 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.