Rroxscaffold_6G00414530

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012954
Physical Location & Seq
Reverse (-)
36840418 .. 36843190
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414530.1

Sequence Viewer

Length: 849 bp
ATGGCAACGGTGGCAGCTGGACCACGTGTCCAGTTGGTGATATCAGATCATTACAATAAGTTCCGTCAGTGTCATAAATGTAGTTGGGTCGCATTTCCACAAAGGTCGAGTCTTTCTTCTTATAAGTATTCCAGTTCATTGTTCTCTGCTTCATCTTTATCTTTGAGTCTTGAAAAGAGGGTGAGGAAGGGTTTTAGGAGAGGAGTGGCTATAACGGCGATGGCGGATTCGGGTACAAGCACAGTCCTTGTCACTGGAGCTGGTGGTAGAACTGGCCAGATAGTTTATAAGAAGTTGAAGGAGAGGTCTGATCAGTATGTTGCTAGAGGTCTGGTTAGAACAGAAGAGAGCAAAGCTAAAATTGGTGGAGCAGATGATCTTTATGTTGGAGATATAACAGATGCCGACAGCATTGTACCTGCGATCCAAGGTATTGATGCCCTCGTGATCCTTACAAGTGCTGTGCCAAAGATGAAACCTTATGATGCAGCCCAGGGTGGGAGGCCCGAGTTCTACTTTGAAGATGGGCAATATCCTGAACAGGTTTGGAAGAGGAAGGCAGAGCAGTACTTGGCTGACTCTGGTATTCCATACACAATCATAAGGGCTGGAGGCTTGCAAGACAAAGAAGGTGGTATCCGTGAACTTCTTATTGGGAAGGATGACGAGCTTCTGAAGACAGAGACAAGAACCATTGCTAGGGCTGACGTCGCAGAAGTCTGCGTTCAGGCATTGCAATTTGAGGAGGCCCAAAACAAGGCATTTGATTTAGCCTCAAAGCCTGAGGGAACCGGAACACCTACCAAAGATTTCAAGGCTCTCTTTTCCCAGATCACCACTCGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

282

Amino Acids

30.87

Weight (kDa)

8.95

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 86 - 154 1.8e-08 NAD(P)H-binding
NAD_binding_10 PF13460 178 - 246 1.1e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 123, 288
AatII GACGTC 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 427
AciI CCGC 1 cut(s) 224
AclWI GGATC 2 cut(s) 418, 442
AcoI YGGCCR 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 695
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 708
AfaI GTAC 3 cut(s) 235, 417, 569
AfiI CCNNNNNNNGG 3 cut(s) 498, 699, 757
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 4 cut(s) 173, 298, 521, 814
AhdI GACNNNNNGTC 1 cut(s) 26
AjnI CCWGG 1 cut(s) 492
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 4 cut(s) 17, 260, 356, 670
AluI AGCT 4 cut(s) 17, 260, 356, 670
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 2 cut(s) 418, 442
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 3 cut(s) 274, 503, 747
ApeKI GCWGC 2 cut(s) 14, 488
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
AspS9I GGNCC 3 cut(s) 20, 504, 748
AsuHPI GGTGA 3 cut(s) 49, 193, 826
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 783
BalI TGGCCA 1 cut(s) 276
BauI CACGAG 1 cut(s) 443
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 683
BbvI GCAGC 2 cut(s) 26, 500
BccI CCATC 2 cut(s) 214, 518
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 494
BciVI GTATCC 1 cut(s) 647
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 2 cut(s) 324, 699
BfuAI ACCTGC 1 cut(s) 427
BfuI GTATCC 1 cut(s) 647
BisI GCNGC 2 cut(s) 15, 489
BlsI GCNGC 2 cut(s) 16, 490
BmcAI AGTACT 1 cut(s) 569
Bme1390I CCNGG 1 cut(s) 494
Bme18I GGWCC 1 cut(s) 20
BmeRI GACNNNNNGTC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 3 cut(s) 20, 504, 748
BmiI GGNNCC 1 cut(s) 790
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 391, 427, 475
BpiI GAAGAC 1 cut(s) 683
BpmI CTGGAG 2 cut(s) 276, 630
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 708
BsaJI CCNNGG 3 cut(s) 427, 492, 493
BsaWI WCCGGW 1 cut(s) 791
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 699, 757
Bse1I ACTGG 4 cut(s) 31, 132, 259, 277
Bse21I CCTNAGG 1 cut(s) 783
Bse3DI GCAATG 2 cut(s) 693, 731
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 3 cut(s) 427, 492, 493
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 3 cut(s) 498, 699, 757
BseMI GCAATG 2 cut(s) 693, 731
BseMII CTCAG 1 cut(s) 774
BseNI ACTGG 4 cut(s) 31, 132, 259, 277
BseRI GAGGAG 2 cut(s) 216, 758
BseXI GCAGC 2 cut(s) 26, 500
BshFI GGCC 3 cut(s) 276, 505, 749
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 1 cut(s) 792
BslI CCNNNNNNNGG 3 cut(s) 498, 699, 757
BsmAI GTCTC 1 cut(s) 677
BsnI GGCC 3 cut(s) 276, 505, 749
BsoBI CYCGRG 1 cut(s) 506
Bsp143I GATC 6 cut(s) 46, 310, 376, 423, 447, 831
BspACI CCGC 1 cut(s) 224
BspANI GGCC 3 cut(s) 276, 505, 749
BspCNI CTCAG 1 cut(s) 775
BspLI GGNNCC 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 2 cut(s) 418, 442
BsrDI GCAATG 2 cut(s) 693, 731
BsrI ACTGG 4 cut(s) 31, 132, 259, 277
BssECI CCNNGG 3 cut(s) 427, 492, 493
BssMI GATC 6 cut(s) 46, 310, 376, 423, 447, 831
BssNI GRCGYC 1 cut(s) 708
BssSI CACGAG 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 2 cut(s) 10, 244
Bst6I CTCTTC 2 cut(s) 339, 545
BstACI GRCGYC 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 617
BstDEI CTNAG 1 cut(s) 783
BstF5I GGATG 1 cut(s) 667
BstKTI GATC 6 cut(s) 49, 313, 379, 426, 450, 834
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 6 cut(s) 46, 310, 376, 423, 447, 831
BstMWI GCNNNNNNNGC 3 cut(s) 11, 215, 710
BstNI CCWGG 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 492
BstV1I GCAGC 2 cut(s) 26, 500
BstV2I GAAGAC 1 cut(s) 683
Bsu36I CCTNAGG 1 cut(s) 783
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 3 cut(s) 276, 505, 749
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 2 cut(s) 74, 252
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 617
Cfr13I GGNCC 3 cut(s) 20, 504, 748
Csp6I GTAC 3 cut(s) 234, 416, 568
CspCI CAANNNNNGTGG 2 cut(s) 613, 648
CviQI GTAC 3 cut(s) 234, 416, 568
DdeI CTNAG 1 cut(s) 783
DpnI GATC 6 cut(s) 48, 312, 378, 425, 449, 833
DpnII GATC 6 cut(s) 46, 310, 376, 423, 447, 831
DriI GACNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 2 cut(s) 339, 545
Eam1105I GACNNNNNGTC 1 cut(s) 26
EarI CTCTTC 2 cut(s) 339, 545
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 427
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 695
Eco72I CACGTG 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 783
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 492
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 15, 489
FokI GGATG 1 cut(s) 674
Fsp4HI GCNGC 2 cut(s) 15, 489
FspBI CTAG 2 cut(s) 324, 699
GluI GCNGC 2 cut(s) 15, 489
GsuI CTGGAG 2 cut(s) 276, 630
HaeIII GGCC 3 cut(s) 276, 505, 749
HapII CCGG 1 cut(s) 792
Hin1I GRCGYC 1 cut(s) 708
HinfI GANTC 4 cut(s) 109, 166, 227, 578
HpaII CCGG 1 cut(s) 792
HphI GGTGA 3 cut(s) 49, 193, 826
Hpy166II GTNNAC 1 cut(s) 644
Hpy188I TCNGA 3 cut(s) 46, 310, 675
Hpy188III TCNNGA 3 cut(s) 170, 445, 536
Hpy8I GTNNAC 1 cut(s) 644
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 5 cut(s) 181, 292, 550, 623, 652
HpyCH4III ACNGT 2 cut(s) 10, 244
HpyCH4IV ACGT 2 cut(s) 25, 708
HpyCH4V TGCA 3 cut(s) 488, 619, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 215, 710
HpyF3I CTNAG 1 cut(s) 783
HpySE526I ACGT 2 cut(s) 25, 708
Hsp92I GRCGYC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 6 cut(s) 46, 310, 376, 423, 447, 831
LmnI GCTCC 2 cut(s) 257, 368
Lsp1109I GCAGC 2 cut(s) 26, 500
LweI GCATC 3 cut(s) 391, 427, 475
MaeI CTAG 2 cut(s) 324, 699
MaeII ACGT 2 cut(s) 25, 708
MaeIII GTNAC 1 cut(s) 250
MalI GATC 6 cut(s) 48, 312, 378, 425, 449, 833
MboI GATC 6 cut(s) 46, 310, 376, 423, 447, 831
MboII GAAGA 5 cut(s) 108, 356, 533, 562, 688
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 2 cut(s) 360, 737
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 3 cut(s) 118, 175, 572
MmeI TCCRAC 1 cut(s) 367
Mox20I TGGCCA 1 cut(s) 276
MscI TGGCCA 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 1 cut(s) 494
MvaI CCWGG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 11, 215, 710
NdeII GATC 6 cut(s) 46, 310, 376, 423, 447, 831
NlaIV GGNNCC 1 cut(s) 790
NmuCI GTSAC 1 cut(s) 250
PasI CCCWGGG 1 cut(s) 493
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 16, 490
PleI GAGTC 3 cut(s) 117, 174, 572
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 3 cut(s) 117, 174, 572
Ppu21I YACGTR 1 cut(s) 26
PsiI TTATAA 2 cut(s) 123, 288
Psp6I CCWGG 1 cut(s) 492
PspCI CACGTG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 790
PspPI GGNCC 3 cut(s) 20, 504, 748
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 3 cut(s) 235, 417, 569
RsaNI GTAC 3 cut(s) 234, 416, 568
SatI GCNGC 2 cut(s) 15, 489
Sau3AI GATC 6 cut(s) 46, 310, 376, 423, 447, 831
Sau96I GGNCC 3 cut(s) 20, 504, 748
ScaI AGTACT 1 cut(s) 569
SchI GAGTC 3 cut(s) 118, 175, 572
ScrFI CCNGG 1 cut(s) 494
SfaNI GCATC 3 cut(s) 391, 427, 475
SinI GGWCC 1 cut(s) 20
Sse9I AATT 2 cut(s) 360, 737
SsiI CCGC 1 cut(s) 224
SspMI CTAG 2 cut(s) 324, 699
StyD4I CCNGG 1 cut(s) 492
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 2 cut(s) 10, 244
TaiI ACGT 2 cut(s) 28, 711
TaqI TCGA 1 cut(s) 107
TasI AATT 2 cut(s) 360, 737
TatI WGTACW 1 cut(s) 567
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 2 cut(s) 74, 259
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 14, 488
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 3 cut(s) 126, 141, 488
TspGWI ACGGA 2 cut(s) 53, 629
TspRI CASTG 2 cut(s) 74, 259
VpaK11BI GGWCC 1 cut(s) 20
XspI CTAG 2 cut(s) 324, 699
ZraI GACGTC 1 cut(s) 709
ZrmI AGTACT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.