pycom04g11880

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012954
Physical Location & Seq
Reverse (-)
14837079 .. 14838503
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11880.1

Sequence Viewer

Length: 516 bp
ATGAAACCTGGATTCGATCCAACTAAAGGTGGGAGGCCTGAGTTCTATTTTGAAGATGGGTCATATCCTGAACAGGTTGATTGGATTGGACAGAAAAACCAAATAGATGCTGCTAAGGCTGCGGGAGTGAAGCAGATTGTGTTGGTTGGGTCCATGGGTGGAACGGATCTTAACAATCCCCTGAACAAATTGGGTGATGGCAATATATTGGTTTGGAAGAGGAAGGCTGAGCAGTATTTGGCTGACTCCGGAATTCCATACACAATCATAAGGGCTGGAGGCTTGCAAGACAAAGAAGGCGGCATCCGTGAACTACTTATAGGGAAGGATGACGAGCTTCTTAAGACCGAAACAAGAACCATTGCTAGACCTGATGTCGCAGAAGTCTGCATTCAGGCACTGCAGTTCGAGGAGGCCAAGTTTAAGGCATTTGATTTAGCCTCAAAGCCGGAGGGAACTGGGACACCGACCAAGGATTTTAAGGCTTTGTTTTCCCAGATCACAACCCGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

172

Amino Acids

18.78

Weight (kDa)

5.34

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 31 - 135 4.3e-21 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 248
AciI CCGC 2 cut(s) 122, 300
AclWI GGATC 2 cut(s) 11, 174
AcsI RAATTY 1 cut(s) 252
AfiI CCNNNNNNNGG 1 cut(s) 26
AflII CTTAAG 1 cut(s) 341
AgsI TTSAA 1 cut(s) 53
AhdI GACNNNNNGTC 1 cut(s) 374
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 1 cut(s) 337
AluI AGCT 1 cut(s) 337
AlwI GGATC 2 cut(s) 11, 174
AlwNI CAGNNNCTG 1 cut(s) 400
Aor13HI TCCGGA 1 cut(s) 248
AoxI GGCC 2 cut(s) 35, 414
ApeKI GCWGC 2 cut(s) 110, 119
ApoI RAATTY 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 1 cut(s) 206
AvaII GGWCC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 97, 106
BccI CCATC 2 cut(s) 50, 191
BciT130I CCWGG 1 cut(s) 9
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 341
BisI GCNGC 3 cut(s) 111, 120, 301
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 3 cut(s) 112, 121, 302
Bme1390I CCNGG 1 cut(s) 9
Bme18I GGWCC 1 cut(s) 150
BmeRI GACNNNNNGTC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 9
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 2 cut(s) 97, 312
BmuI ACTGGG 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 153, 471
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 1 cut(s) 360
BseAI TCCGGA 1 cut(s) 248
BseBI CCWGG 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 153, 471
BseGI GGATG 2 cut(s) 303, 334
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 2 cut(s) 30, 219
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 2 cut(s) 97, 106
BshFI GGCC 2 cut(s) 37, 416
BsiSI CCGG 2 cut(s) 249, 449
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 2 cut(s) 37, 416
Bsp13I TCCGGA 1 cut(s) 248
Bsp143I GATC 3 cut(s) 16, 166, 498
Bsp1720I GCTNAGC 1 cut(s) 228
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 2 cut(s) 122, 300
BspANI GGCC 2 cut(s) 37, 416
BspCNI CTCAG 2 cut(s) 31, 220
BspEI TCCGGA 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 405
BspPI GGATC 2 cut(s) 11, 174
BspTI CTTAAG 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 360
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 153, 471
BssMI GATC 3 cut(s) 16, 166, 498
BssT1I CCWWGG 2 cut(s) 153, 471
Bst2UI CCWGG 1 cut(s) 9
Bst6I CTCTTC 1 cut(s) 212
BstAFI CTTAAG 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 3 cut(s) 39, 114, 228
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 303, 334
BstKTI GATC 3 cut(s) 19, 169, 501
BstMBI GATC 3 cut(s) 16, 166, 498
BstMWI GCNNNNNNNGC 2 cut(s) 116, 119
BstNI CCWGG 1 cut(s) 9
BstSCI CCNGG 1 cut(s) 7
BstSFI CTRYAG 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 97, 106
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 2 cut(s) 37, 416
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 2 cut(s) 303, 334
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 150
CviAII CATG 1 cut(s) 154
DdeI CTNAG 3 cut(s) 39, 114, 228
DpnI GATC 3 cut(s) 18, 168, 500
DpnII GATC 3 cut(s) 16, 166, 498
DriI GACNNNNNGTC 1 cut(s) 374
Eam1104I CTCTTC 1 cut(s) 212
Eam1105I GACNNNNNGTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 212
Eco130I CCWWGG 2 cut(s) 153, 471
Eco147I AGGCCT 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 150
EcoRI GAATTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 7
EcoT14I CCWWGG 2 cut(s) 153, 471
ErhI CCWWGG 2 cut(s) 153, 471
FaeI CATG 1 cut(s) 157
FaiI YATR 6 cut(s) 64, 155, 206, 259, 269, 320
FaqI GGGAC 1 cut(s) 475
FatI CATG 1 cut(s) 153
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 111, 120, 301
FokI GGATG 2 cut(s) 290, 341
Fsp4HI GCNGC 3 cut(s) 111, 120, 301
FspBI CTAG 1 cut(s) 366
GluI GCNGC 3 cut(s) 111, 120, 301
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 2 cut(s) 37, 416
HapII CCGG 2 cut(s) 249, 449
Hin1II CATG 1 cut(s) 157
HinfI GANTC 2 cut(s) 12, 245
HpaII CCGG 2 cut(s) 249, 449
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 311
Hpy188III TCNNGA 2 cut(s) 68, 249
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 3 cut(s) 217, 290, 319
HpyCH4V TGCA 3 cut(s) 286, 390, 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 119
HpyF3I CTNAG 3 cut(s) 39, 114, 228
Hsp92II CATG 1 cut(s) 157
Kpn2I TCCGGA 1 cut(s) 248
Kzo9I GATC 3 cut(s) 16, 166, 498
Lsp1109I GCAGC 2 cut(s) 97, 106
LweI GCATC 2 cut(s) 97, 312
MaeI CTAG 1 cut(s) 366
MalI GATC 3 cut(s) 18, 168, 500
MboI GATC 3 cut(s) 16, 166, 498
MboII GAAGA 2 cut(s) 65, 229
MflI RGATCY 1 cut(s) 166
MluCI AATT 2 cut(s) 188, 252
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 7 cut(s) 27, 213, 272, 403, 406, 445, 451
MroI TCCGGA 1 cut(s) 248
MseI TTAA 4 cut(s) 171, 342, 423, 480
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 2 cut(s) 249, 449
MspR9I CCNGG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 2 cut(s) 116, 119
NcoI CCATGG 1 cut(s) 153
NdeII GATC 3 cut(s) 16, 166, 498
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 37
PctI GAATGC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 12
PkrI GCNGC 3 cut(s) 112, 121, 302
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 1 cut(s) 150
PstI CTGCAG 1 cut(s) 405
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 1 cut(s) 166
SaqAI TTAA 4 cut(s) 171, 342, 423, 480
SatI GCNGC 3 cut(s) 111, 120, 301
Sau3AI GATC 3 cut(s) 16, 166, 498
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 9
SetI ASST 5 cut(s) 10, 31, 78, 339, 373
SfaNI GCATC 2 cut(s) 97, 312
SfcI CTRYAG 1 cut(s) 401
SinI GGWCC 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 2 cut(s) 188, 252
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 2 cut(s) 122, 300
SspMI CTAG 1 cut(s) 366
StuI AGGCCT 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 7
StyI CCWWGG 2 cut(s) 153, 471
TaqI TCGA 2 cut(s) 15, 408
TaqII GACCGA 1 cut(s) 362
TasI AATT 2 cut(s) 188, 252
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 4 cut(s) 171, 342, 423, 480
Tru9I TTAA 4 cut(s) 171, 342, 423, 480
TscAI CASTG 1 cut(s) 405
TseI GCWGC 2 cut(s) 110, 119
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 179, 296
TspRI CASTG 1 cut(s) 405
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 150
XapI RAATTY 1 cut(s) 252
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.