MD12G1143700.v1.1

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012954
Physical Location & Seq
Reverse (-)
22273925 .. 22278145
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1143700.v1.1.491

Sequence Viewer

Length: 969 bp
ATGATGGCAACGGTGGCGGTGGCACCACGTGTCCCCTTGGTTATCTCTAATAATGGCATCCATAAATGTCAAAAATGTAGTTGGGTGGCAGTTCCAGAAAGATCGACCCTTTCTTTTCAATCCACTTCATTTTCTGCTGTATCCTTGTTTATCTATATAAGGGCGAGAAGGGGTTTTGGGAGAGGAGTTGTCACGAAGGCCATGGCGGATTCGGGTGCAAGCATCGTCCTTGTCACTGGAGCTGGTGGTAGAACTGGTAAACTAGTTTATAAGAAGTTAAAGGAGAGGTCAGACCAGTATGTTGCTAGGGGTCTGGTTAGGACTGAAGAGAGCAAACAGAGCATTGGCGGAGCAGATGATCTTTATGTTGGAGATATAAGGGATGCTGACAGCATTCTACCTGCGATCCAAGGCATTGATGCTCTCATAATCCTTACAAGCGCTGTGCCTAAGATGAAACCTGGCTTTGATCCAACCAAAGGTGGGAGGCCTGAGTTCTATTTTGAAGACGGGTCGTATCCTGAACAGGTTGATTGGATTGGACAGAAGAACCAAATAGATGCTGCTAAGGCTGCGGGAGCGAAGCAGATTGTGTTAGTTGGGTCTATGGGTGGAACAGATCTTAACAATCCCCTGAACAAATTGGGTAATGGCAACATATTGGTTTGGAAGAGGAAGGCTGAGCAGTATTTGGCTGACTCCGGAATTCCATACACAATCATAAGGGCTGGAGGCTTGCAAGACAAAGAAGGAGGCAACCGTGAACTACTTATAGGGAAGGATGACGAGCTTCTTAAGACCGAAACAAGAACCATTGCTAGACCTGATGTCGCAGAAGTCTGCATTCAGGCACTGCAGTTCGAGGAGGCCAAGTTCAAGGCTTTTGATTTGGCCTCAAAGCCGGAGGGAACTGGGACACCGACCAAGGATTTTAAGGCTTTATTTTCCCAGATCACTACCCGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

323

Amino Acids

34.82

Weight (kDa)

9.16

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 74 - 241 3.4e-06 NmrA-like family
NAD_binding_10 PF13460 80 - 286 4.4e-33 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 701
AciI CCGC 4 cut(s) 17, 206, 348, 575
AclWI GGATC 2 cut(s) 400, 464
AcsI RAATTY 1 cut(s) 705
AcuI CTGAAG 1 cut(s) 345
AcvI CACGTG 1 cut(s) 29
AdeI CACNNNGTG 1 cut(s) 29
AfeI AGCGCT 1 cut(s) 442
AfiI CCNNNNNNNGG 2 cut(s) 479, 483
AflII CTTAAG 1 cut(s) 794
AflIII ACRYGT 1 cut(s) 28
AgsI TTSAA 3 cut(s) 119, 506, 877
AhdI GACNNNNNGTC 1 cut(s) 827
AhlI ACTAGT 1 cut(s) 262
AjnI CCWGG 1 cut(s) 460
AloI GAACNNNNNNTCC 2 cut(s) 857, 889
AluBI AGCT 2 cut(s) 242, 790
AluI AGCT 2 cut(s) 242, 790
AlwI GGATC 2 cut(s) 400, 464
AlwNI CAGNNNCTG 1 cut(s) 853
Aor13HI TCCGGA 1 cut(s) 701
Aor51HI AGCGCT 1 cut(s) 442
AoxI GGCC 4 cut(s) 198, 488, 867, 891
ApeKI GCWGC 2 cut(s) 563, 572
ApoI RAATTY 1 cut(s) 705
AspLEI GCGC 1 cut(s) 443
BanI GGYRCC 1 cut(s) 22
BbrPI CACGTG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 513
BbvI GCAGC 2 cut(s) 550, 559
BciT130I CCWGG 1 cut(s) 462
BciVI GTATCC 2 cut(s) 151, 528
BcuI ACTAGT 1 cut(s) 262
BfaI CTAG 3 cut(s) 263, 306, 819
BfmI CTRYAG 1 cut(s) 854
BfoI RGCGCY 1 cut(s) 444
BfrI CTTAAG 1 cut(s) 794
BfuAI ACCTGC 1 cut(s) 409
BfuI GTATCC 2 cut(s) 151, 528
BglII AGATCT 1 cut(s) 619
BisI GCNGC 2 cut(s) 564, 573
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 2 cut(s) 565, 574
Bme1390I CCNGG 1 cut(s) 462
BmeRI GACNNNNNGTC 1 cut(s) 827
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 462
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 5 cut(s) 66, 231, 373, 409, 550
BmuI ACTGGG 1 cut(s) 921
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 2 cut(s) 258, 750
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 681
BsaAI YACGTR 1 cut(s) 29
BsaJI CCNNGG 4 cut(s) 36, 201, 409, 924
BsaWI WCCGGW 1 cut(s) 701
BsaXI ACNNNNNCTCC 2 cut(s) 857, 887
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 483
Bse1I ACTGG 4 cut(s) 241, 259, 295, 916
Bse3DI GCAATG 1 cut(s) 813
BseAI TCCGGA 1 cut(s) 701
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 36, 201, 409, 924
BseGI GGATG 3 cut(s) 57, 388, 787
BseLI CCNNNNNNNGG 2 cut(s) 479, 483
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 2 cut(s) 483, 672
BseNI ACTGG 4 cut(s) 241, 259, 295, 916
BseRI GAGGAG 2 cut(s) 198, 878
BseXI GCAGC 2 cut(s) 550, 559
BshFI GGCC 4 cut(s) 200, 490, 869, 893
BshNI GGYRCC 1 cut(s) 22
BsiSI CCGG 2 cut(s) 702, 902
BslFI GGGAC 2 cut(s) 17, 928
BslI CCNNNNNNNGG 2 cut(s) 479, 483
BsmFI GGGAC 2 cut(s) 17, 928
BsmI GAATGC 2 cut(s) 393, 843
BsnI GGCC 4 cut(s) 200, 490, 869, 893
Bsp13I TCCGGA 1 cut(s) 701
Bsp143I GATC 6 cut(s) 101, 358, 405, 469, 619, 951
Bsp1720I GCTNAGC 1 cut(s) 681
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 4 cut(s) 17, 206, 348, 575
BspANI GGCC 4 cut(s) 200, 490, 869, 893
BspCNI CTCAG 2 cut(s) 484, 673
BspEI TCCGGA 1 cut(s) 701
BspLI GGNNCC 1 cut(s) 24
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 2 cut(s) 400, 464
BspT107I GGYRCC 1 cut(s) 22
BspTI CTTAAG 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 813
BsrI ACTGG 4 cut(s) 241, 259, 295, 916
BssECI CCNNGG 4 cut(s) 36, 201, 409, 924
BssMI GATC 6 cut(s) 101, 358, 405, 469, 619, 951
BssT1I CCWWGG 4 cut(s) 36, 201, 409, 924
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 13, 761
Bst6I CTCTTC 2 cut(s) 321, 665
BstAFI CTTAAG 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 220, 737
BstDEI CTNAG 4 cut(s) 450, 492, 567, 681
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 57, 388, 787
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 6 cut(s) 104, 361, 408, 472, 622, 954
BstMBI GATC 6 cut(s) 101, 358, 405, 469, 619, 951
BstMWI GCNNNNNNNGC 5 cut(s) 14, 339, 569, 572, 578
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 1 cut(s) 854
BstV1I GCAGC 2 cut(s) 550, 559
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
BsuI GTATCC 2 cut(s) 151, 528
BsuRI GGCC 4 cut(s) 200, 490, 869, 893
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 3 cut(s) 57, 388, 787
BtsI GCAGTG 1 cut(s) 851
BtsIMutI CAGTG 2 cut(s) 234, 851
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 220, 737
CaiI CAGNNNCTG 1 cut(s) 853
CfoI GCGC 1 cut(s) 443
CviAII CATG 1 cut(s) 202
DdeI CTNAG 4 cut(s) 450, 492, 567, 681
DpnI GATC 6 cut(s) 103, 360, 407, 471, 621, 953
DpnII GATC 6 cut(s) 101, 358, 405, 469, 619, 951
DraIII CACNNNGTG 1 cut(s) 29
DriI GACNNNNNGTC 1 cut(s) 827
Eam1104I CTCTTC 2 cut(s) 321, 665
Eam1105I GACNNNNNGTC 1 cut(s) 827
EarI CTCTTC 2 cut(s) 321, 665
EciI GGCGGA 2 cut(s) 221, 363
Eco130I CCWWGG 4 cut(s) 36, 201, 409, 924
Eco147I AGGCCT 1 cut(s) 490
Eco47III AGCGCT 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 345
Eco72I CACGTG 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 460
EcoT14I CCWWGG 4 cut(s) 36, 201, 409, 924
ErhI CCWWGG 4 cut(s) 36, 201, 409, 924
FaeI CATG 1 cut(s) 205
FaqI GGGAC 2 cut(s) 17, 928
FatI CATG 1 cut(s) 201
FauI CCCGC 1 cut(s) 568
Fnu4HI GCNGC 2 cut(s) 564, 573
FokI GGATG 3 cut(s) 44, 395, 794
Fsp4HI GCNGC 2 cut(s) 564, 573
FspBI CTAG 3 cut(s) 263, 306, 819
GlaI GCGC 1 cut(s) 442
GluI GCNGC 2 cut(s) 564, 573
GsuI CTGGAG 2 cut(s) 258, 750
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 4 cut(s) 200, 490, 869, 893
HapII CCGG 2 cut(s) 702, 902
HhaI GCGC 1 cut(s) 443
Hin1II CATG 1 cut(s) 205
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HinfI GANTC 2 cut(s) 209, 698
HpaII CCGG 2 cut(s) 702, 902
Hpy166II GTNNAC 2 cut(s) 260, 764
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 4 cut(s) 95, 193, 521, 702
Hpy8I GTNNAC 2 cut(s) 260, 764
HpyAV CCTTC 5 cut(s) 162, 190, 670, 743, 772
HpyCH4III ACNGT 2 cut(s) 13, 761
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 4 cut(s) 218, 739, 843, 856
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 339, 569, 572, 578
HpyF3I CTNAG 4 cut(s) 450, 492, 567, 681
HpySE526I ACGT 1 cut(s) 28
Hsp92II CATG 1 cut(s) 205
HspAI GCGC 1 cut(s) 441
Kpn2I TCCGGA 1 cut(s) 701
Kzo9I GATC 6 cut(s) 101, 358, 405, 469, 619, 951
LmnI GCTCC 3 cut(s) 239, 350, 578
Lsp1109I GCAGC 2 cut(s) 550, 559
LweI GCATC 5 cut(s) 66, 231, 373, 409, 550
MaeI CTAG 3 cut(s) 263, 306, 819
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 2 cut(s) 190, 232
MalI GATC 6 cut(s) 103, 360, 407, 471, 621, 953
MboI GATC 6 cut(s) 101, 358, 405, 469, 619, 951
MboII GAAGA 4 cut(s) 338, 518, 559, 682
MflI RGATCY 1 cut(s) 619
MluCI AATT 2 cut(s) 641, 705
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 2 cut(s) 349, 497
MroI TCCGGA 1 cut(s) 701
MseI TTAA 4 cut(s) 278, 624, 795, 933
MspCI CTTAAG 1 cut(s) 794
MspI CCGG 2 cut(s) 702, 902
MspR9I CCNGG 1 cut(s) 462
Mva1269I GAATGC 2 cut(s) 393, 843
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 5 cut(s) 14, 339, 569, 572, 578
NcoI CCATGG 1 cut(s) 201
NdeII GATC 6 cut(s) 101, 358, 405, 469, 619, 951
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 2 cut(s) 190, 232
PceI AGGCCT 1 cut(s) 490
PctI GAATGC 2 cut(s) 393, 843
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 2 cut(s) 565, 574
PleI GAGTC 1 cut(s) 692
PmaCI CACGTG 1 cut(s) 29
PmlI CACGTG 1 cut(s) 29
PpsI GAGTC 1 cut(s) 692
Ppu21I YACGTR 1 cut(s) 29
PsiI TTATAA 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 460
PspCI CACGTG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 1 cut(s) 24
PstI CTGCAG 1 cut(s) 858
PstNI CAGNNNCTG 1 cut(s) 853
PsuI RGATCY 1 cut(s) 619
SaqAI TTAA 4 cut(s) 278, 624, 795, 933
SatI GCNGC 2 cut(s) 564, 573
Sau3AI GATC 6 cut(s) 101, 358, 405, 469, 619, 951
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 1 cut(s) 462
SetI ASST 9 cut(s) 31, 244, 290, 403, 463, 484, 531, 792, 826
SfaNI GCATC 5 cut(s) 66, 231, 373, 409, 550
SfcI CTRYAG 1 cut(s) 854
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SpeI ACTAGT 1 cut(s) 262
Sse9I AATT 2 cut(s) 641, 705
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 4 cut(s) 17, 206, 348, 575
SspMI CTAG 3 cut(s) 263, 306, 819
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 4 cut(s) 36, 201, 409, 924
TaaI ACNGT 2 cut(s) 13, 761
TaiI ACGT 1 cut(s) 31
TaqI TCGA 2 cut(s) 104, 861
TaqII GACCGA 1 cut(s) 815
TasI AATT 2 cut(s) 641, 705
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 4 cut(s) 278, 624, 795, 933
Tru9I TTAA 4 cut(s) 278, 624, 795, 933
TscAI CASTG 2 cut(s) 241, 858
TseFI GTSAC 2 cut(s) 190, 232
TseI GCWGC 2 cut(s) 563, 572
Tsp45I GTSAC 2 cut(s) 190, 232
TspDTI ATGAA 2 cut(s) 117, 470
TspRI CASTG 2 cut(s) 241, 858
Vha464I CTTAAG 1 cut(s) 794
XapI RAATTY 1 cut(s) 705
XspI CTAG 3 cut(s) 263, 306, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.