Rh3BG149100

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0012954
Physical Location & Seq
Forward (+)
12646914 .. 12649335
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG149100.1

Sequence Viewer

Length: 987 bp
ATGGCAACGGTGGCAGCTGGACCACGTGTCCAGTTGGTGATATCAGATCATTACAATAATTTCCGTCAGTGTCATAAATGTAGTTGGGTCGCATTTCCACAAAGGTCGAGTCTTTCTTCTTATAAGTATTCCAGTTCATTGTTCTCTGCTTCATCTTTATCTTTGAGTCTTGAAAAGAGGGTGAGGAAGGGTTTTAGGAGAGGAGTGGCTATAACGGCGATGGCGGATTCGGGTACAAGCACAGTCCTTGTCACTGGAGCTGGTGGCAGAACTGGCAACATAGTTTATAAGAAGTTGAAGGAGAGGTCTGATCAGTATGTTGCTAGAGGTCTGGTTAGAACAGAAGAGAGCAAAGCTAAAATTGGTGGATCAGATGATCTTTATGTTGGAGATATAACAGATGCCGACAGCATTGTACCTGCGATCCAAGGTATCGATGCCCTCGTGATCCTTACAAGTGCTGTGCCAAAGATGAAACCTGGTTATGATCCAAGCAAGGGTGAGAGGCCCGAGTTCTACTTTGAAGATGGGCAATATCCTGAACAGGTTGATTGGCTTGGACAGAAAAACCAAATTGATGCTGCTAAGGCTGCGGGAGTGAAGCAAATTGTATTGGTTGGGTCTATGGGTGGAACTGATTTGAGTAACCCCCTAAACAGCTTGGGTAATGGAAATATCTTGGTTTGGAAGAGGAAGGCAGAGCAGTACTTGGCTGACTCTGGTATTCCATACACAATCATAAGGGCTGGAGGCTTGCAAGACAAACAGGGTGGTGTTCGTGAACTTCTTATTGGGAAAGATGATGAGCTTCTGAAGACGGAGACAAGAACCATTACTAGGGCTGACGTCGCAGAAGTCTGCGTTCAGGCATTGCAATTTGAGGAGGCCAAAAACAAGGCATTTGATTTAGCCTCCAAGCCTGAGGGAACCGGAACACCTACCAAAGATTTCAAGGCTCTCTTTTCCCAGATCACCACTCGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

328

Amino Acids

35.6

Weight (kDa)

9.03

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 79 - 247 1.1e-08 NmrA-like family
NAD_binding_10 PF13460 86 - 292 1.8e-34 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 123, 288
AatII GACGTC 1 cut(s) 849
Acc36I ACCTGC 1 cut(s) 427
AciI CCGC 2 cut(s) 224, 593
AclWI GGATC 4 cut(s) 376, 418, 442, 482
AcuI CTGAAG 1 cut(s) 833
AcvI CACGTG 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 846
AfaI GTAC 3 cut(s) 235, 417, 707
AfiI CCNNNNNNNGG 2 cut(s) 497, 837
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 4 cut(s) 173, 298, 524, 952
AhdI GACNNNNNGTC 1 cut(s) 26
AjnI CCWGG 1 cut(s) 478
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AluBI AGCT 5 cut(s) 17, 260, 356, 660, 808
AluI AGCT 5 cut(s) 17, 260, 356, 660, 808
Alw26I GTCTC 1 cut(s) 815
AlwI GGATC 4 cut(s) 376, 418, 442, 482
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 2 cut(s) 506, 885
ApeKI GCWGC 3 cut(s) 14, 581, 590
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
AspS9I GGNCC 2 cut(s) 20, 507
AsuHPI GGTGA 4 cut(s) 49, 193, 512, 964
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 921
BauI CACGAG 1 cut(s) 443
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 821
BbvI GCAGC 3 cut(s) 26, 568, 577
BccI CCATC 2 cut(s) 214, 521
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 480
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 815
BfaI CTAG 2 cut(s) 324, 837
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 3 cut(s) 15, 582, 591
BlsI GCNGC 3 cut(s) 16, 583, 592
BmcAI AGTACT 1 cut(s) 707
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 1 cut(s) 20
BmeRI GACNNNNNGTC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 20, 507
BmiI GGNNCC 1 cut(s) 928
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 3 cut(s) 391, 427, 568
BpiI GAAGAC 1 cut(s) 821
BpmI CTGGAG 2 cut(s) 276, 768
Bpu10I CCTNAGC 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 846
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 1 cut(s) 929
Bsc4I CCNNNNNNNGG 2 cut(s) 497, 837
Bse1I ACTGG 4 cut(s) 31, 132, 259, 277
Bse21I CCTNAGG 1 cut(s) 921
Bse3DI GCAATG 1 cut(s) 869
BseBI CCWGG 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 497, 837
BseMI GCAATG 1 cut(s) 869
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 4 cut(s) 31, 132, 259, 277
BseRI GAGGAG 2 cut(s) 216, 896
BseXI GCAGC 3 cut(s) 26, 568, 577
BshFI GGCC 2 cut(s) 508, 887
BshVI ATCGAT 1 cut(s) 435
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 1 cut(s) 930
BslI CCNNNNNNNGG 2 cut(s) 497, 837
BsmAI GTCTC 1 cut(s) 815
BsnI GGCC 2 cut(s) 508, 887
BsoBI CYCGRG 1 cut(s) 509
Bsp143I GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
BspACI CCGC 2 cut(s) 224, 593
BspANI GGCC 2 cut(s) 508, 887
BspCNI CTCAG 1 cut(s) 913
BspDI ATCGAT 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 928
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 4 cut(s) 376, 418, 442, 482
BsrDI GCAATG 1 cut(s) 869
BsrI ACTGG 4 cut(s) 31, 132, 259, 277
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
BssNI GRCGYC 1 cut(s) 846
BssSI CACGAG 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 10, 244
Bst6I CTCTTC 2 cut(s) 339, 683
BstACI GRCGYC 1 cut(s) 846
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 755
BstDEI CTNAG 2 cut(s) 585, 921
BstKTI GATC 8 cut(s) 49, 313, 371, 379, 426, 450, 490, 972
BstMAI GTCTC 1 cut(s) 815
BstMBI GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
BstMWI GCNNNNNNNGC 6 cut(s) 11, 215, 273, 587, 590, 848
BstNI CCWGG 1 cut(s) 480
BstSCI CCNGG 1 cut(s) 478
BstV1I GCAGC 3 cut(s) 26, 568, 577
BstV2I GAAGAC 1 cut(s) 821
Bsu15I ATCGAT 1 cut(s) 435
Bsu36I CCTNAGG 1 cut(s) 921
BsuRI GGCC 2 cut(s) 508, 887
BsuTUI ATCGAT 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 233
BtsIMutI CAGTG 2 cut(s) 74, 252
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 755
Cfr13I GGNCC 2 cut(s) 20, 507
ClaI ATCGAT 1 cut(s) 435
CsiI ACCWGGT 1 cut(s) 478
Csp6I GTAC 3 cut(s) 234, 416, 706
CspCI CAANNNNNGTGG 2 cut(s) 751, 786
CviQI GTAC 3 cut(s) 234, 416, 706
DdeI CTNAG 2 cut(s) 585, 921
DpnI GATC 8 cut(s) 48, 312, 370, 378, 425, 449, 489, 971
DpnII GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
DriI GACNNNNNGTC 1 cut(s) 26
Eam1104I CTCTTC 2 cut(s) 339, 683
Eam1105I GACNNNNNGTC 1 cut(s) 26
EarI CTCTTC 2 cut(s) 339, 683
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 427
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 833
Eco72I CACGTG 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 921
Eco88I CYCGRG 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 478
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FalI AAGNNNNNCTT 2 cut(s) 944, 976
FauI CCCGC 1 cut(s) 586
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 15, 582, 591
Fsp4HI GCNGC 3 cut(s) 15, 582, 591
FspBI CTAG 2 cut(s) 324, 837
GluI GCNGC 3 cut(s) 15, 582, 591
GsuI CTGGAG 2 cut(s) 276, 768
HaeIII GGCC 2 cut(s) 508, 887
HapII CCGG 1 cut(s) 930
Hin1I GRCGYC 1 cut(s) 846
HinfI GANTC 4 cut(s) 109, 166, 227, 716
HpaII CCGG 1 cut(s) 930
HphI GGTGA 4 cut(s) 49, 193, 512, 964
Hpy166II GTNNAC 1 cut(s) 782
Hpy188I TCNGA 4 cut(s) 46, 310, 373, 813
Hpy188III TCNNGA 4 cut(s) 170, 445, 539, 779
Hpy8I GTNNAC 1 cut(s) 782
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 3 cut(s) 181, 292, 688
HpyCH4III ACNGT 2 cut(s) 10, 244
HpyCH4IV ACGT 2 cut(s) 25, 846
HpyCH4V TGCA 2 cut(s) 757, 874
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 215, 273, 587, 590, 848
HpyF3I CTNAG 2 cut(s) 585, 921
HpySE526I ACGT 2 cut(s) 25, 846
Hsp92I GRCGYC 1 cut(s) 846
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 3 cut(s) 26, 568, 577
LweI GCATC 3 cut(s) 391, 427, 568
MabI ACCWGGT 1 cut(s) 478
MaeI CTAG 2 cut(s) 324, 837
MaeII ACGT 2 cut(s) 25, 846
MaeIII GTNAC 2 cut(s) 250, 644
MalI GATC 8 cut(s) 48, 312, 370, 378, 425, 449, 489, 971
MboI GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
MboII GAAGA 5 cut(s) 108, 356, 536, 700, 826
MluCI AATT 5 cut(s) 58, 360, 573, 606, 875
MlyI GAGTC 3 cut(s) 118, 175, 710
MmeI TCCRAC 1 cut(s) 367
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 930
MspR9I CCNGG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 6 cut(s) 11, 215, 273, 587, 590, 848
NdeII GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
NlaIV GGNNCC 1 cut(s) 928
NmuCI GTSAC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 3 cut(s) 16, 583, 592
PleI GAGTC 3 cut(s) 117, 174, 710
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 3 cut(s) 117, 174, 710
Ppu21I YACGTR 1 cut(s) 26
PsiI TTATAA 2 cut(s) 123, 288
Psp6I CCWGG 1 cut(s) 478
PspCI CACGTG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 928
PspPI GGNCC 2 cut(s) 20, 507
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 3 cut(s) 235, 417, 707
RsaNI GTAC 3 cut(s) 234, 416, 706
SatI GCNGC 3 cut(s) 15, 582, 591
Sau3AI GATC 8 cut(s) 46, 310, 368, 376, 423, 447, 487, 969
Sau96I GGNCC 2 cut(s) 20, 507
ScaI AGTACT 1 cut(s) 707
SchI GAGTC 3 cut(s) 118, 175, 710
ScrFI CCNGG 1 cut(s) 480
SexAI ACCWGGT 1 cut(s) 478
SfaNI GCATC 3 cut(s) 391, 427, 568
SinI GGWCC 1 cut(s) 20
Sse9I AATT 5 cut(s) 58, 360, 573, 606, 875
SsiI CCGC 2 cut(s) 224, 593
SspMI CTAG 2 cut(s) 324, 837
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 2 cut(s) 10, 244
TaiI ACGT 2 cut(s) 28, 849
TaqI TCGA 2 cut(s) 107, 435
TasI AATT 5 cut(s) 58, 360, 573, 606, 875
TatI WGTACW 1 cut(s) 705
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 2 cut(s) 74, 259
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 3 cut(s) 14, 581, 590
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 3 cut(s) 126, 141, 488
TspGWI ACGGA 2 cut(s) 53, 833
TspRI CASTG 2 cut(s) 74, 259
VpaK11BI GGWCC 1 cut(s) 20
XspI CTAG 2 cut(s) 324, 837
ZraI GACGTC 1 cut(s) 847
ZrmI AGTACT 1 cut(s) 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.