RLG00000024580

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012954
Physical Location & Seq
Reverse (-)
37215997 .. 37218448
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024580

Sequence Viewer

Length: 984 bp
ATGGCAACGGTGGCAGCTGGACCACGTGTCCAGTTGGTGATATCAGGACATTACAATAAGTTCCGTCAATGTCATAAATGTAGTTGGGTCGCATTTCCTCAGAGGTCGAGTCTTTCTTCTTATAAGTATTCCAGTTCATTGTTCTCTGCTTCATCTTTATCTTTGAGTCTTGAAAAGAGGGTGAGGAAGGGTTTTAGGAGAGGAGTGGCTATAACGGCGATGGCGGATTCGGGTACAAGCACAGTCCTTGTCACTGGAGCTGGTGGTAGAACTGGCCAGATAGTTTATAAGAAGTTGAAGGAGAGGTCTGATCAGTATGTTGCTAGAGGTCTGGTTAGAACAGAAGAGAGCAAAGCTAAAATTGGTGGATCAGATGATCTTTATGTTGGAGATATAACAGATGCCGACAGCATTGTACCTGCGATCCAAGGTATCGATGCCCTCGTGATCCTTACAAGTGCTGTGCCAAAGATGAAAACCTATGATCCAACCAAGGGTGGGAGGCCTGAGTTCTACTTTGAAGATGGGCAATTTCCTGAACAGGTTGATTGGCTTGGACAGAAAAACCAAATTGATGCTGCTAAGGCCGCGGGAGTGAAGCAAATTGTATTGGTTGGGTCTATGGGTGGAACTGATTTGAGTAACCCCCTAAACAGCTTGGGTAATGGAAATATCTTGGTTTGGAAGAGGAAGGCAGAGCAGTACTTGGCTGACTCTGGTATTCCATACACAATCATAAGGGCTGGAGGCTTGCAAGACAAAGAAGGTGGTAACCGTGAACTTCTTATTGGGAAGGATGACGAGCTTCTGAAGACAGAGACAAGAACCATTCCCAGGGCTGATGTTGCAGAAGTCTGCGTTCAGGCATTGCAATTTGAGGAGGCCAAAAACAAGGCATTTGATTTAGCCTCAAAGCCTGAGGGAAAGGGAACACCTACCAAAGATTTCAAGGCTCTCTTTTCCCAGATCACCACTCGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

328

Amino Acids

35.48

Weight (kDa)

9.29

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 79 - 246 1.1e-08 NmrA-like family
NAD_binding_10 PF13460 86 - 291 1.6e-35 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 123, 288
Acc36I ACCTGC 1 cut(s) 427
AccII CGCG 1 cut(s) 590
AciI CCGC 3 cut(s) 224, 588, 590
AclWI GGATC 4 cut(s) 376, 418, 442, 479
AcoI YGGCCR 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 830
AcvI CACGTG 1 cut(s) 26
AfaI GTAC 3 cut(s) 235, 417, 704
AfiI CCNNNNNNNGG 3 cut(s) 494, 498, 834
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 4 cut(s) 173, 298, 521, 949
AhdI GACNNNNNGTC 1 cut(s) 26
AjnI CCWGG 1 cut(s) 833
AjuI GAANNNNNNNTTGG 2 cut(s) 771, 803
AluBI AGCT 5 cut(s) 17, 260, 356, 657, 805
AluI AGCT 5 cut(s) 17, 260, 356, 657, 805
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 4 cut(s) 376, 418, 442, 479
AoxI GGCC 4 cut(s) 274, 503, 585, 882
ApeKI GCWGC 2 cut(s) 14, 578
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 3 cut(s) 49, 193, 961
AvaII GGWCC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 918
BalI TGGCCA 1 cut(s) 276
BauI CACGAG 1 cut(s) 443
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 818
BbvI GCAGC 2 cut(s) 26, 565
BccI CCATC 2 cut(s) 214, 518
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 835
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 3 cut(s) 15, 579, 588
BlsI GCNGC 3 cut(s) 16, 580, 589
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 1 cut(s) 835
Bme18I GGWCC 1 cut(s) 20
BmeRI GACNNNNNGTC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 20
BmrFI CCNGG 1 cut(s) 835
BmsI GCATC 3 cut(s) 391, 427, 565
BpiI GAAGAC 1 cut(s) 818
BpmI CTGGAG 2 cut(s) 276, 765
Bpu10I CCTNAGC 1 cut(s) 582
Bsa29I ATCGAT 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 427, 492, 588, 833, 834
Bsc4I CCNNNNNNNGG 3 cut(s) 494, 498, 834
Bse1I ACTGG 4 cut(s) 31, 132, 259, 277
Bse21I CCTNAGG 1 cut(s) 918
Bse3DI GCAATG 1 cut(s) 866
BseBI CCWGG 1 cut(s) 835
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 5 cut(s) 427, 492, 588, 833, 834
BseGI GGATG 1 cut(s) 802
BseLI CCNNNNNNNGG 3 cut(s) 494, 498, 834
BseMI GCAATG 1 cut(s) 866
BseMII CTCAG 3 cut(s) 113, 498, 909
BseNI ACTGG 4 cut(s) 31, 132, 259, 277
BseRI GAGGAG 2 cut(s) 216, 893
BseXI GCAGC 2 cut(s) 26, 565
Bsh1236I CGCG 1 cut(s) 590
BshFI GGCC 4 cut(s) 276, 505, 587, 884
BshVI ATCGAT 1 cut(s) 435
BslI CCNNNNNNNGG 3 cut(s) 494, 498, 834
BsmAI GTCTC 1 cut(s) 812
BsnI GGCC 4 cut(s) 276, 505, 587, 884
Bsp143I GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
BspACI CCGC 3 cut(s) 224, 588, 590
BspANI GGCC 4 cut(s) 276, 505, 587, 884
BspCNI CTCAG 3 cut(s) 112, 499, 910
BspDI ATCGAT 1 cut(s) 435
BspFNI CGCG 1 cut(s) 590
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 4 cut(s) 376, 418, 442, 479
BsrDI GCAATG 1 cut(s) 866
BsrI ACTGG 4 cut(s) 31, 132, 259, 277
BssECI CCNNGG 5 cut(s) 427, 492, 588, 833, 834
BssMI GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
BssSI CACGAG 1 cut(s) 443
BssT1I CCWWGG 2 cut(s) 427, 492
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 835
Bst4CI ACNGT 3 cut(s) 10, 244, 776
Bst6I CTCTTC 2 cut(s) 339, 680
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 752
BstDEI CTNAG 4 cut(s) 99, 507, 582, 918
BstDSI CCRYGG 1 cut(s) 588
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 1 cut(s) 802
BstFNI CGCG 1 cut(s) 590
BstKTI GATC 7 cut(s) 313, 371, 379, 426, 450, 487, 969
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
BstMWI GCNNNNNNNGC 5 cut(s) 11, 215, 584, 587, 845
BstNI CCWGG 1 cut(s) 835
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 833
BstUI CGCG 1 cut(s) 590
BstV1I GCAGC 2 cut(s) 26, 565
BstV2I GAAGAC 1 cut(s) 818
Bsu15I ATCGAT 1 cut(s) 435
Bsu36I CCTNAGG 1 cut(s) 918
BsuRI GGCC 4 cut(s) 276, 505, 587, 884
BsuTUI ATCGAT 1 cut(s) 435
BtgI CCRYGG 1 cut(s) 588
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 1 cut(s) 802
BtsIMutI CAGTG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 752
Cfr13I GGNCC 1 cut(s) 20
Cfr42I CCGCGG 1 cut(s) 591
ClaI ATCGAT 1 cut(s) 435
Csp6I GTAC 3 cut(s) 234, 416, 703
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviQI GTAC 3 cut(s) 234, 416, 703
DdeI CTNAG 4 cut(s) 99, 507, 582, 918
DpnI GATC 7 cut(s) 312, 370, 378, 425, 449, 486, 968
DpnII GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
DriI GACNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 2 cut(s) 339, 680
Eam1105I GACNNNNNGTC 1 cut(s) 26
EarI CTCTTC 2 cut(s) 339, 680
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 2 cut(s) 427, 492
Eco147I AGGCCT 1 cut(s) 505
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 830
Eco72I CACGTG 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 918
Eco91I GGTNACC 1 cut(s) 770
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 833
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 2 cut(s) 427, 492
ErhI CCWWGG 2 cut(s) 427, 492
FalI AAGNNNNNCTT 2 cut(s) 941, 973
FauI CCCGC 1 cut(s) 583
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 15, 579, 588
FokI GGATG 1 cut(s) 809
Fsp4HI GCNGC 3 cut(s) 15, 579, 588
FspBI CTAG 1 cut(s) 324
GluI GCNGC 3 cut(s) 15, 579, 588
GsuI CTGGAG 2 cut(s) 276, 765
HaeIII GGCC 4 cut(s) 276, 505, 587, 884
HinfI GANTC 4 cut(s) 109, 166, 227, 713
HphI GGTGA 3 cut(s) 49, 193, 961
Hpy166II GTNNAC 1 cut(s) 779
Hpy188I TCNGA 4 cut(s) 102, 310, 373, 810
Hpy188III TCNNGA 4 cut(s) 45, 170, 445, 536
Hpy8I GTNNAC 1 cut(s) 779
HpyAV CCTTC 5 cut(s) 181, 292, 685, 758, 787
HpyCH4III ACNGT 3 cut(s) 10, 244, 776
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 3 cut(s) 754, 848, 871
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 215, 584, 587, 845
HpyF3I CTNAG 4 cut(s) 99, 507, 582, 918
HpySE526I ACGT 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 310
KspI CCGCGG 1 cut(s) 591
Kzo9I GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 26, 565
LweI GCATC 3 cut(s) 391, 427, 565
MaeI CTAG 1 cut(s) 324
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 3 cut(s) 250, 641, 770
MalI GATC 7 cut(s) 312, 370, 378, 425, 449, 486, 968
MboI GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
MboII GAAGA 5 cut(s) 108, 356, 533, 697, 823
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 5 cut(s) 360, 530, 570, 603, 872
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 3 cut(s) 118, 175, 707
MmeI TCCRAC 2 cut(s) 367, 512
Mox20I TGGCCA 1 cut(s) 276
MscI TGGCCA 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 2 cut(s) 17, 590
MspR9I CCNGG 1 cut(s) 835
MvaI CCWGG 1 cut(s) 835
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 5 cut(s) 11, 215, 584, 587, 845
NdeII GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
NmuCI GTSAC 1 cut(s) 250
PasI CCCWGGG 1 cut(s) 834
PceI AGGCCT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 3 cut(s) 16, 580, 589
PleI GAGTC 3 cut(s) 117, 174, 707
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 3 cut(s) 117, 174, 707
Ppu21I YACGTR 1 cut(s) 26
PsiI TTATAA 2 cut(s) 123, 288
Psp6I CCWGG 1 cut(s) 833
PspCI CACGTG 1 cut(s) 26
PspEI GGTNACC 1 cut(s) 770
PspGI CCWGG 1 cut(s) 833
PspPI GGNCC 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 3 cut(s) 235, 417, 704
RsaNI GTAC 3 cut(s) 234, 416, 703
SacII CCGCGG 1 cut(s) 591
SatI GCNGC 3 cut(s) 15, 579, 588
Sau3AI GATC 7 cut(s) 310, 368, 376, 423, 447, 484, 966
Sau96I GGNCC 1 cut(s) 20
ScaI AGTACT 1 cut(s) 704
SchI GAGTC 3 cut(s) 118, 175, 707
ScrFI CCNGG 1 cut(s) 835
SfaNI GCATC 3 cut(s) 391, 427, 565
Sfr303I CCGCGG 1 cut(s) 591
SgrBI CCGCGG 1 cut(s) 591
SinI GGWCC 1 cut(s) 20
Sse9I AATT 5 cut(s) 360, 530, 570, 603, 872
SseBI AGGCCT 1 cut(s) 505
SsiI CCGC 3 cut(s) 224, 588, 590
SspMI CTAG 1 cut(s) 324
StuI AGGCCT 1 cut(s) 505
StyD4I CCNGG 1 cut(s) 833
StyI CCWWGG 2 cut(s) 427, 492
TaaI ACNGT 3 cut(s) 10, 244, 776
TaiI ACGT 1 cut(s) 28
TaqI TCGA 2 cut(s) 107, 435
TasI AATT 5 cut(s) 360, 530, 570, 603, 872
TatI WGTACW 1 cut(s) 702
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 14, 578
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 3 cut(s) 126, 141, 488
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 20
XspI CTAG 1 cut(s) 324
ZrmI AGTACT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.